AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062808

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062808.t1 MTR_2g071700 90.033 301 30 0 1 301 38 338 0.0 548
MS.gene062808.t1 MTR_6g034190 57.407 486 180 5 2 472 38 511 0.0 540
MS.gene062808.t1 MTR_2g072240 49.894 473 180 5 2 472 31 448 4.33e-153 443
MS.gene062808.t1 MTR_2g070780 72.757 301 79 1 1 301 37 334 2.73e-149 430
MS.gene062808.t1 MTR_2g071720 82.873 181 15 1 308 472 1 181 1.05e-104 310
MS.gene062808.t1 MTR_2g070660 84.426 122 19 0 38 159 45 166 1.74e-69 219
MS.gene062808.t1 MTR_2g070820 74.775 111 28 0 242 352 1 111 9.13e-52 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062808.t1 AT1G80960 23.810 441 256 14 2 419 71 454 2.65e-14 75.5
MS.gene062808.t1 AT1G80960 23.810 441 256 14 2 419 71 454 2.65e-14 75.5
MS.gene062808.t1 AT1G80960 23.810 441 256 14 2 419 71 454 2.65e-14 75.5
MS.gene062808.t1 AT1G80960 23.810 441 256 14 2 419 32 415 2.75e-14 75.1
MS.gene062808.t1 AT1G80960 26.644 289 154 9 2 277 71 314 1.06e-13 73.2
MS.gene062808.t1 AT1G80960 26.644 289 154 9 2 277 71 314 1.45e-13 73.2

Find 84 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGAATGTGCTCAGGATTT+TGG 0.185156 2.3:-24868339 MS.gene062808:CDS
CTTACCCTGGTGGTTTGTTT+TGG 0.236904 2.3:-24867078 MS.gene062808:CDS
GTTAAGTACTTAATATTAAA+TGG 0.248591 2.3:-24866965 MS.gene062808:CDS
TTCAAATCAATTGACAATTT+AGG 0.254356 2.3:+24866845 None:intergenic
CAACTCAAGAACCTTAAAAT+TGG 0.260477 2.3:+24868574 None:intergenic
CCAAGCGATGGGAGTCATTA+TGG 0.279125 2.3:-24869244 MS.gene062808:CDS
CTAAAGTCTGTTTGTATAAA+GGG 0.291958 2.3:-24867019 MS.gene062808:CDS
GCACGAAGAACTTGAAGTTT+TGG 0.293746 2.3:+24868242 None:intergenic
CGGATATTGTGGGGTGGTTA+AGG 0.294599 2.3:-24868727 MS.gene062808:CDS
AAGACTTCAACATTTGTCTT+TGG 0.296399 2.3:+24869195 None:intergenic
TTTAAAGTCTGCTGGGAATC+AGG 0.299645 2.3:+24868521 None:intergenic
CCTAAAGTCTGTTTGTATAA+AGG 0.317402 2.3:-24867020 MS.gene062808:CDS
ATCTTCATGACTACAAATAA+TGG 0.327696 2.3:+24868280 None:intergenic
CCTTTATACAAACAGACTTT+AGG 0.331850 2.3:+24867020 None:intergenic
TTAAGGAAAATACTGGAAAA+AGG 0.338514 2.3:-24868710 MS.gene062808:CDS
TCAAAATACTCCTCATTTGT+AGG 0.358011 2.3:+24868854 None:intergenic
TCTTAGGGTCATCGAGGTTC+AGG 0.358731 2.3:-24867525 MS.gene062808:intron
GACAATTTAGGAGAGAGTTT+TGG 0.359150 2.3:+24866857 None:intergenic
TATTTGTAGTCATGAAGATT+TGG 0.359911 2.3:-24868276 MS.gene062808:CDS
TTTGTCTTTGGTCAAAACTA+AGG 0.395796 2.3:+24869207 None:intergenic
TCATGAAGATTTGGTCTTTG+AGG 0.413287 2.3:-24868267 MS.gene062808:CDS
TCAAATATTGACAATCATAT+TGG 0.420752 2.3:-24868800 MS.gene062808:CDS
ACCGAGTCTATACAGAAGTA+TGG 0.427830 2.3:-24866815 MS.gene062808:CDS
TTATGAATAATATTACCATT+TGG 0.432439 2.3:-24866937 MS.gene062808:CDS
TCGGATGGTACAACTCTTCA+AGG 0.444461 2.3:+24868775 None:intergenic
GTTTGTATAAAGGGATATAA+AGG 0.451854 2.3:-24867010 MS.gene062808:CDS
TCTTAAACTCTAGGTCAAGT+TGG 0.476404 2.3:-24867134 MS.gene062808:intron
TTGTCAATTGATTTGAAACA+TGG 0.480319 2.3:-24866839 MS.gene062808:CDS
TTTGAAGATTCATTAGTTGT+GGG 0.487958 2.3:+24867403 None:intergenic
TCCGCACTTGCACAACTCTC+AGG 0.492078 2.3:+24868746 None:intergenic
ATTTGAAGATTCATTAGTTG+TGG 0.494505 2.3:+24867402 None:intergenic
CAAAACAAACCACCAGGGTA+AGG 0.497256 2.3:+24867079 None:intergenic
CTTTGGTCAAAACTAAGGTC+AGG 0.499026 2.3:+24869212 None:intergenic
TTTGTAATATGAATGTGCTC+AGG 0.508079 2.3:-24868346 MS.gene062808:CDS
TTAAAGTCTGCTGGGAATCA+GGG 0.509876 2.3:+24868522 None:intergenic
TTGTGCAAGTGCGGATATTG+TGG 0.521452 2.3:-24868738 MS.gene062808:CDS
AATCACAATTCTCGACGGTA+AGG 0.523805 2.3:+24868420 None:intergenic
TGTGCAAGTGCGGATATTGT+GGG 0.527611 2.3:-24868737 MS.gene062808:CDS
CAAGTGTGCTTTCCAAGCGA+TGG 0.528071 2.3:-24869256 MS.gene062808:CDS
AACCTCAAAAGGTAAATCAA+TGG 0.528655 2.3:+24868601 None:intergenic
CCATAATGACTCCCATCGCT+TGG 0.530097 2.3:+24869244 None:intergenic
TGGTGGCAGCTTTGATGAAT+GGG 0.531188 2.3:-24866795 MS.gene062808:CDS
AAATTGGAGAAAACCTCAAA+AGG 0.535849 2.3:+24868590 None:intergenic
ATGGTGGCAGCTTTGATGAA+TGG 0.540826 2.3:-24866796 MS.gene062808:CDS
GGCTCAAAAGGGACCCCTGT+AGG 0.543884 2.3:-24867057 MS.gene062808:CDS
GCTACGGCTAACACACCTAC+AGG 0.546526 2.3:+24867042 None:intergenic
TACAAACAGACTTTAGGCTA+CGG 0.550195 2.3:+24867026 None:intergenic
GATAGTCTTCCTTACCCTGG+TGG 0.551228 2.3:-24867088 MS.gene062808:CDS
TGTCAATTGATTTGAAACAT+GGG 0.552653 2.3:-24866838 MS.gene062808:CDS
GGTCATCGAGGTTCAGGCAT+GGG 0.558503 2.3:-24867519 MS.gene062808:CDS
CACAACTCTCAGGCAAGTGT+CGG 0.563469 2.3:+24868756 None:intergenic
GGGGTGGTTAAGGAAAATAC+TGG 0.572493 2.3:-24868717 MS.gene062808:CDS
TACTGTTCAATTCAAAGTTG+AGG 0.576864 2.3:+24867466 None:intergenic
AAGTGTGCTTTCCAAGCGAT+GGG 0.578750 2.3:-24869255 MS.gene062808:CDS
TAACGAAGGAATATTAGACT+TGG 0.583947 2.3:-24868645 MS.gene062808:CDS
TTGAGCCAAAACAAACCACC+AGG 0.586250 2.3:+24867073 None:intergenic
GAGTGCAAAAGAAAGAGCTA+TGG 0.595456 2.3:+24867434 None:intergenic
AGTCTGCTGGGAATCAGGGG+AGG 0.596089 2.3:+24868526 None:intergenic
TGTATAAAGGGATATAAAGG+TGG 0.601016 2.3:-24867007 MS.gene062808:CDS
TCTTGTGATTATCTTAACGA+AGG 0.601820 2.3:-24868659 MS.gene062808:CDS
GAATCTTCAAATAAATAACC+AGG 0.602091 2.3:-24867391 MS.gene062808:intron
GTCTGCTGGGAATCAGGGGA+GGG 0.602271 2.3:+24868527 None:intergenic
TAAAGTCTGCTGGGAATCAG+GGG 0.602508 2.3:+24868523 None:intergenic
AATATTTGATGTAATCATCA+TGG 0.606995 2.3:+24868814 None:intergenic
ATTGTAAGACGGTCATCAAG+TGG 0.611036 2.3:+24869146 None:intergenic
ACAGGTAGCTCCTACAAATG+AGG 0.611347 2.3:-24868864 MS.gene062808:intron
TACAATGGCTATGCAACGCA+AGG 0.612851 2.3:-24869129 MS.gene062808:intron
ACCATACTTCTGTATAGACT+CGG 0.615500 2.3:+24866814 None:intergenic
CTACGGCTAACACACCTACA+GGG 0.616738 2.3:+24867043 None:intergenic
GGGTCATCGAGGTTCAGGCA+TGG 0.625995 2.3:-24867520 MS.gene062808:CDS
GTTGCATAGCCATTGTAAGA+CGG 0.627198 2.3:+24869135 None:intergenic
CGGATGGTACAACTCTTCAA+GGG 0.643395 2.3:+24868776 None:intergenic
GCCTGAGAGTTGTGCAAGTG+CGG 0.646523 2.3:-24868747 MS.gene062808:CDS
ACTTGATGACCGTCTTACAA+TGG 0.648825 2.3:-24869144 MS.gene062808:CDS
ACTCTCAGGCAAGTGTCGGA+TGG 0.653394 2.3:+24868760 None:intergenic
TAAGAAATCACAATTCTCGA+CGG 0.656792 2.3:+24868415 None:intergenic
AATGATAGTCTTCCTTACCC+TGG 0.661903 2.3:-24867091 MS.gene062808:CDS
AAGTACTTAATATTAAATGG+CGG 0.681402 2.3:-24866962 MS.gene062808:CDS
TGATTGTCTTAGGGTCATCG+AGG 0.687412 2.3:-24867531 MS.gene062808:intron
GAGTCTATACAGAAGTATGG+TGG 0.708284 2.3:-24866812 MS.gene062808:CDS
GTGCAAGTGCGGATATTGTG+GGG 0.710817 2.3:-24868736 MS.gene062808:CDS
TACGGCTAACACACCTACAG+GGG 0.715804 2.3:+24867044 None:intergenic
CAAGTGCGGATATTGTGGGG+TGG 0.717451 2.3:-24868733 MS.gene062808:CDS
TGAGCCAAAACAAACCACCA+GGG 0.739963 2.3:+24867074 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATAATTAGATTATTCAAA+TGG - chr2.3:24867020-24867039 MS.gene062808:CDS 15.0%
!! GTTAAGTACTTAATATTAAA+TGG - chr2.3:24869085-24869104 MS.gene062808:intron 15.0%
!! TTATGAATAATATTACCATT+TGG - chr2.3:24869113-24869132 MS.gene062808:intron 15.0%
!!! AGAGATATTATAGAAATTTT+CGG - chr2.3:24867895-24867914 MS.gene062808:intron 15.0%
!!! ATTTTAATATATTTCAGACT+TGG + chr2.3:24867685-24867704 None:intergenic 15.0%
!!! CAATTATTCAAGTTTTAAAA+TGG - chr2.3:24868742-24868761 MS.gene062808:CDS 15.0%
!! AAGTACTTAATATTAAATGG+CGG - chr2.3:24869088-24869107 MS.gene062808:intron 20.0%
!! AATATTTGATGTAATCATCA+TGG + chr2.3:24867239-24867258 None:intergenic 20.0%
!! ATATCATAGCTTATTATATG+TGG - chr2.3:24868235-24868254 MS.gene062808:CDS 20.0%
!! CATTCATTTCATTTAGAAAA+TGG + chr2.3:24867110-24867129 None:intergenic 20.0%
!! TATATGTACAACTTAACATA+TGG - chr2.3:24868057-24868076 MS.gene062808:intron 20.0%
!! TCAAATATTGACAATCATAT+TGG - chr2.3:24867250-24867269 MS.gene062808:intron 20.0%
!! TGAGATATGATATATATTGA+CGG - chr2.3:24867151-24867170 MS.gene062808:intron 20.0%
!! TTCAAATCAATTGACAATTT+AGG + chr2.3:24869208-24869227 None:intergenic 20.0%
!!! ATAAGCTATGATATTTTTCT+AGG + chr2.3:24868229-24868248 None:intergenic 20.0%
!!! CAAAAATTTAGAACATTAGA+TGG + chr2.3:24868022-24868041 None:intergenic 20.0%
!!! GGAGAAATTTTATTTATTGA+TGG - chr2.3:24867853-24867872 MS.gene062808:intron 20.0%
!!! TAATATTACCATTTGGTTTT+TGG - chr2.3:24869120-24869139 MS.gene062808:intron 20.0%
!!! TACAATGATTTTCAAATTCT+TGG - chr2.3:24867832-24867851 MS.gene062808:intron 20.0%
!!! TTTTAGATGACATTTTTCTA+AGG + chr2.3:24868388-24868407 None:intergenic 20.0%
! AATAAATAACCAGGTATGTT+TGG - chr2.3:24868668-24868687 MS.gene062808:CDS 25.0%
! AGAAAGATAGTTACAAAACA+AGG + chr2.3:24868499-24868518 None:intergenic 25.0%
! ATAAATAACCAGGTATGTTT+GGG - chr2.3:24868669-24868688 MS.gene062808:CDS 25.0%
! ATCTTCATGACTACAAATAA+TGG + chr2.3:24867773-24867792 None:intergenic 25.0%
! ATTTGAAGATTCATTAGTTG+TGG + chr2.3:24868651-24868670 None:intergenic 25.0%
! CATTTCATTTAGAAAATGGA+AGG + chr2.3:24867106-24867125 None:intergenic 25.0%
! CTAAAGTCTGTTTGTATAAA+GGG - chr2.3:24869031-24869050 MS.gene062808:intron 25.0%
! GAAAGATAGTTACAAAACAA+GGG + chr2.3:24868498-24868517 None:intergenic 25.0%
! GAATCTTCAAATAAATAACC+AGG - chr2.3:24868659-24868678 MS.gene062808:CDS 25.0%
! GTTTGTATAAAGGGATATAA+AGG - chr2.3:24869040-24869059 MS.gene062808:intron 25.0%
! TAAAGATAGTTGCAAACATA+TGG + chr2.3:24868705-24868724 None:intergenic 25.0%
! TAAGGAAAATACTGGAAAAA+GGG - chr2.3:24867341-24867360 MS.gene062808:intron 25.0%
! TATCTTTCTTGATTGTCTTA+GGG - chr2.3:24868510-24868529 MS.gene062808:CDS 25.0%
! TATTTGTAGTCATGAAGATT+TGG - chr2.3:24867774-24867793 MS.gene062808:intron 25.0%
! TTAAGGAAAATACTGGAAAA+AGG - chr2.3:24867340-24867359 MS.gene062808:intron 25.0%
! TTTGAAGATTCATTAGTTGT+GGG + chr2.3:24868650-24868669 None:intergenic 25.0%
!! ATTGATTTATCGTTACATCA+TGG + chr2.3:24868850-24868869 None:intergenic 25.0%
!! GATATTATAGAAATTTTCGG+TGG - chr2.3:24867898-24867917 MS.gene062808:intron 25.0%
!! TTAAGTTGTACATATATCCA+AGG + chr2.3:24868053-24868072 None:intergenic 25.0%
!!! ATGCTGAAAATTGTTGTTTA+TGG - chr2.3:24868281-24868300 MS.gene062808:CDS 25.0%
!!! TGTCAATTGATTTGAAACAT+GGG - chr2.3:24869212-24869231 MS.gene062808:CDS 25.0%
!!! TTGTCAATTGATTTGAAACA+TGG - chr2.3:24869211-24869230 MS.gene062808:CDS 25.0%
AAATTAATCCCAAACATACC+TGG + chr2.3:24868680-24868699 None:intergenic 30.0%
AAATTGGAGAAAACCTCAAA+AGG + chr2.3:24867463-24867482 None:intergenic 30.0%
AACCTCAAAAGGTAAATCAA+TGG + chr2.3:24867452-24867471 None:intergenic 30.0%
AAGACTTCAACATTTGTCTT+TGG + chr2.3:24866858-24866877 None:intergenic 30.0%
AAGGAAAATACTGGAAAAAG+GGG - chr2.3:24867342-24867361 MS.gene062808:intron 30.0%
AAGGAATCATACTTGCATTA+AGG + chr2.3:24868165-24868184 None:intergenic 30.0%
ACATTCATCCAAAAACCAAA+TGG + chr2.3:24869131-24869150 None:intergenic 30.0%
AGGAATCATACTTGCATTAA+GGG + chr2.3:24868164-24868183 None:intergenic 30.0%
ATATTTGTAGTAGGCATACT+TGG + chr2.3:24868331-24868350 None:intergenic 30.0%
CAACTCAAGAACCTTAAAAT+TGG + chr2.3:24867479-24867498 None:intergenic 30.0%
CACAAAAAATCACGCTTATT+GGG - chr2.3:24868195-24868214 MS.gene062808:CDS 30.0%
CCTAAAGTCTGTTTGTATAA+AGG - chr2.3:24869030-24869049 MS.gene062808:intron 30.0%
CCTTTATACAAACAGACTTT+AGG + chr2.3:24869033-24869052 None:intergenic 30.0%
CTATCTTTCTTGATTGTCTT+AGG - chr2.3:24868509-24868528 MS.gene062808:CDS 30.0%
CTCTAAGCAATATTTGTAGT+AGG + chr2.3:24868340-24868359 None:intergenic 30.0%
TAACGAAGGAATATTAGACT+TGG - chr2.3:24867405-24867424 MS.gene062808:CDS 30.0%
TAAGAAATCACAATTCTCGA+CGG + chr2.3:24867638-24867657 None:intergenic 30.0%
TACTGTTCAATTCAAAGTTG+AGG + chr2.3:24868587-24868606 None:intergenic 30.0%
TCAAAATACTCCTCATTTGT+AGG + chr2.3:24867199-24867218 None:intergenic 30.0%
TCACAAAAAATCACGCTTAT+TGG - chr2.3:24868194-24868213 MS.gene062808:CDS 30.0%
TCTAAGGAGCAAAATCAAAT+TGG + chr2.3:24868372-24868391 None:intergenic 30.0%
TCTTGTGATTATCTTAACGA+AGG - chr2.3:24867391-24867410 MS.gene062808:intron 30.0%
TGTATAAAGGGATATAAAGG+TGG - chr2.3:24869043-24869062 MS.gene062808:intron 30.0%
TTTCTTAACATGTGTCATGA+AGG - chr2.3:24868457-24868476 MS.gene062808:CDS 30.0%
TTTGTAATATGAATGTGCTC+AGG - chr2.3:24867704-24867723 MS.gene062808:intron 30.0%
TTTGTCTTTGGTCAAAACTA+AGG + chr2.3:24866846-24866865 None:intergenic 30.0%
! AAATTTTTGTTGCATCTCCT+TGG - chr2.3:24868033-24868052 MS.gene062808:intron 30.0%
! CATTTTTCCTCTTAAACTCT+AGG - chr2.3:24868907-24868926 MS.gene062808:intron 30.0%
! TGAGGTTTTCTCCAATTTTA+AGG - chr2.3:24867465-24867484 MS.gene062808:CDS 30.0%
!! GTTTTTGGATGAATGTTCAT+GGG - chr2.3:24869135-24869154 MS.gene062808:CDS 30.0%
!!! AAGTGTTTTTAAAGTCTGCT+GGG + chr2.3:24867539-24867558 None:intergenic 30.0%
!!! ATAAGCGTGATTTTTTGTGA+TGG + chr2.3:24868194-24868213 None:intergenic 30.0%
!!! GTTTTAAAATGGCGTGTAAT+AGG - chr2.3:24868753-24868772 MS.gene062808:CDS 30.0%
!!! TAAGTGTTTTTAAAGTCTGC+TGG + chr2.3:24867540-24867559 None:intergenic 30.0%
!!! TTTTTTGTGATGGCTCAAAA+AGG + chr2.3:24868184-24868203 None:intergenic 30.0%
AACTTGACCTAGAGTTTAAG+AGG + chr2.3:24868917-24868936 None:intergenic 35.0%
ACCATACTTCTGTATAGACT+CGG + chr2.3:24869239-24869258 None:intergenic 35.0%
AGATGCAAAAAAGTGAGTCA+AGG - chr2.3:24868117-24868136 MS.gene062808:intron 35.0%
TACAAACAGACTTTAGGCTA+CGG + chr2.3:24869027-24869046 None:intergenic 35.0%
TACTTGGTTACTGTTCAGTA+AGG + chr2.3:24868315-24868334 None:intergenic 35.0%
TCTTAAACTCTAGGTCAAGT+TGG - chr2.3:24868916-24868935 MS.gene062808:intron 35.0%
! ACGAAGAACTTGAAGTTTTG+GGG + chr2.3:24867809-24867828 None:intergenic 35.0%
! AGAAATTTTCGGTGGTATTC+TGG - chr2.3:24867906-24867925 MS.gene062808:intron 35.0%
! ATTTTCATGAAGAGTCAACC+AGG - chr2.3:24867048-24867067 MS.gene062808:CDS 35.0%
! CACGAAGAACTTGAAGTTTT+GGG + chr2.3:24867810-24867829 None:intergenic 35.0%
! GACAATTTAGGAGAGAGTTT+TGG + chr2.3:24869196-24869215 None:intergenic 35.0%
! TATGAATGTGCTCAGGATTT+TGG - chr2.3:24867711-24867730 MS.gene062808:intron 35.0%
! TCATGAAGATTTGGTCTTTG+AGG - chr2.3:24867783-24867802 MS.gene062808:intron 35.0%
!! GGTTTTTGGATGAATGTTCA+TGG - chr2.3:24869134-24869153 MS.gene062808:CDS 35.0%
!!! GACCATTGATTTACCTTTTG+AGG - chr2.3:24867447-24867466 MS.gene062808:CDS 35.0%
!!! TGGTTTGTTTTGGCTCAAAA+GGG - chr2.3:24868982-24869001 MS.gene062808:intron 35.0%
AATCACAATTCTCGACGGTA+AGG + chr2.3:24867633-24867652 None:intergenic 40.0%
AATGATAGTCTTCCTTACCC+TGG - chr2.3:24868959-24868978 MS.gene062808:intron 40.0%
ACCGAGTCTATACAGAAGTA+TGG - chr2.3:24869235-24869254 MS.gene062808:CDS 40.0%
ACTGGAAAAAGGGGTGATTA+AGG - chr2.3:24867351-24867370 MS.gene062808:intron 40.0%
AGATTCTTTACAACCCTTCC+TGG + chr2.3:24867069-24867088 None:intergenic 40.0%
AGCATCGCATCACAATTACT+CGG + chr2.3:24868266-24868285 None:intergenic 40.0%
ATGGCGTGTAATAGGAATTG+TGG - chr2.3:24868761-24868780 MS.gene062808:CDS 40.0%
ATTGTAAGACGGTCATCAAG+TGG + chr2.3:24866907-24866926 None:intergenic 40.0%
CTTTGGTCAAAACTAAGGTC+AGG + chr2.3:24866841-24866860 None:intergenic 40.0%
GAGTGCAAAAGAAAGAGCTA+TGG + chr2.3:24868619-24868638 None:intergenic 40.0%
GTTGCATAGCCATTGTAAGA+CGG + chr2.3:24866918-24866937 None:intergenic 40.0%
TTAAAGTCTGCTGGGAATCA+GGG + chr2.3:24867531-24867550 None:intergenic 40.0%
TTGGTTACTGTTCAGTAAGG+TGG + chr2.3:24868312-24868331 None:intergenic 40.0%
! ACTTGATGACCGTCTTACAA+TGG - chr2.3:24866906-24866925 MS.gene062808:CDS 40.0%
! GAGTCTATACAGAAGTATGG+TGG - chr2.3:24869238-24869257 MS.gene062808:CDS 40.0%
! GCACGAAGAACTTGAAGTTT+TGG + chr2.3:24867811-24867830 None:intergenic 40.0%
! TTTAAAGTCTGCTGGGAATC+AGG + chr2.3:24867532-24867551 None:intergenic 40.0%
!!! GTGGTTTGTTTTGGCTCAAA+AGG - chr2.3:24868981-24869000 MS.gene062808:intron 40.0%
AAGTGTGCTTTCCAAGCGAT+GGG - chr2.3:24866795-24866814 MS.gene062808:CDS 45.0%
ACAGGTAGCTCCTACAAATG+AGG - chr2.3:24867186-24867205 MS.gene062808:intron 45.0%
CAAAACAAACCACCAGGGTA+AGG + chr2.3:24868974-24868993 None:intergenic 45.0%
CATGAAGAGTCAACCAGGAA+GGG - chr2.3:24867053-24867072 MS.gene062808:CDS 45.0%
CGGATGGTACAACTCTTCAA+GGG + chr2.3:24867277-24867296 None:intergenic 45.0%
GGGGTGGTTAAGGAAAATAC+TGG - chr2.3:24867333-24867352 MS.gene062808:intron 45.0%
TAAAGTCTGCTGGGAATCAG+GGG + chr2.3:24867530-24867549 None:intergenic 45.0%
TACAATGGCTATGCAACGCA+AGG - chr2.3:24866921-24866940 MS.gene062808:CDS 45.0%
TCATGAAGAGTCAACCAGGA+AGG - chr2.3:24867052-24867071 MS.gene062808:CDS 45.0%
TCGGATGGTACAACTCTTCA+AGG + chr2.3:24867278-24867297 None:intergenic 45.0%
TGACGGAATTCATGCATGAC+AGG - chr2.3:24867168-24867187 MS.gene062808:intron 45.0%
TGAGCCAAAACAAACCACCA+GGG + chr2.3:24868979-24868998 None:intergenic 45.0%
TGATTGTCTTAGGGTCATCG+AGG - chr2.3:24868519-24868538 MS.gene062808:CDS 45.0%
TGTGCAAGTGCGGATATTGT+GGG - chr2.3:24867313-24867332 MS.gene062808:intron 45.0%
TTGAGCCAAAACAAACCACC+AGG + chr2.3:24868980-24868999 None:intergenic 45.0%
TTGTGCAAGTGCGGATATTG+TGG - chr2.3:24867312-24867331 MS.gene062808:intron 45.0%
! ATGGTGGCAGCTTTGATGAA+TGG - chr2.3:24869254-24869273 MS.gene062808:CDS 45.0%
! TGGTGGCAGCTTTGATGAAT+GGG - chr2.3:24869255-24869274 MS.gene062808:CDS 45.0%
!!! CTTACCCTGGTGGTTTGTTT+TGG - chr2.3:24868972-24868991 MS.gene062808:intron 45.0%
CAAGTGTGCTTTCCAAGCGA+TGG - chr2.3:24866794-24866813 MS.gene062808:CDS 50.0%
CACAACTCTCAGGCAAGTGT+CGG + chr2.3:24867297-24867316 None:intergenic 50.0%
CCAAGCGATGGGAGTCATTA+TGG - chr2.3:24866806-24866825 MS.gene062808:CDS 50.0%
CCATAATGACTCCCATCGCT+TGG + chr2.3:24866809-24866828 None:intergenic 50.0%
CGGATATTGTGGGGTGGTTA+AGG - chr2.3:24867323-24867342 MS.gene062808:intron 50.0%
CTACGGCTAACACACCTACA+GGG + chr2.3:24869010-24869029 None:intergenic 50.0%
GATAGTCTTCCTTACCCTGG+TGG - chr2.3:24868962-24868981 MS.gene062808:intron 50.0%
GTGCAAGTGCGGATATTGTG+GGG - chr2.3:24867314-24867333 MS.gene062808:intron 50.0%
TACGGCTAACACACCTACAG+GGG + chr2.3:24869009-24869028 None:intergenic 50.0%
TCTTAGGGTCATCGAGGTTC+AGG - chr2.3:24868525-24868544 MS.gene062808:CDS 50.0%
CAAGTGCGGATATTGTGGGG+TGG - chr2.3:24867317-24867336 MS.gene062808:intron 55.0%
GCCTGAGAGTTGTGCAAGTG+CGG - chr2.3:24867303-24867322 MS.gene062808:intron 55.0%
GCTACGGCTAACACACCTAC+AGG + chr2.3:24869011-24869030 None:intergenic 55.0%
GGTCATCGAGGTTCAGGCAT+GGG - chr2.3:24868531-24868550 MS.gene062808:CDS 55.0%
TCCGCACTTGCACAACTCTC+AGG + chr2.3:24867307-24867326 None:intergenic 55.0%
!! ACTCTCAGGCAAGTGTCGGA+TGG + chr2.3:24867293-24867312 None:intergenic 55.0%
AGTCTGCTGGGAATCAGGGG+AGG + chr2.3:24867527-24867546 None:intergenic 60.0%
GGCTCAAAAGGGACCCCTGT+AGG - chr2.3:24868993-24869012 MS.gene062808:intron 60.0%
GGGTCATCGAGGTTCAGGCA+TGG - chr2.3:24868530-24868549 MS.gene062808:CDS 60.0%
GTCTGCTGGGAATCAGGGGA+GGG + chr2.3:24867526-24867545 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 24866778 24869294 24866778 ID=MS.gene062808
chr2.3 mRNA 24866778 24869294 24866778 ID=MS.gene062808.t1;Parent=MS.gene062808
chr2.3 exon 24869130 24869294 24869130 ID=MS.gene062808.t1.exon1;Parent=MS.gene062808.t1
chr2.3 CDS 24869130 24869294 24869130 ID=cds.MS.gene062808.t1;Parent=MS.gene062808.t1
chr2.3 exon 24868145 24868882 24868145 ID=MS.gene062808.t1.exon2;Parent=MS.gene062808.t1
chr2.3 CDS 24868145 24868882 24868145 ID=cds.MS.gene062808.t1;Parent=MS.gene062808.t1
chr2.3 exon 24867392 24867541 24867392 ID=MS.gene062808.t1.exon3;Parent=MS.gene062808.t1
chr2.3 CDS 24867392 24867541 24867392 ID=cds.MS.gene062808.t1;Parent=MS.gene062808.t1
chr2.3 exon 24866778 24867143 24866778 ID=MS.gene062808.t1.exon4;Parent=MS.gene062808.t1
chr2.3 CDS 24866778 24867143 24866778 ID=cds.MS.gene062808.t1;Parent=MS.gene062808.t1
Gene Sequence

>MS.gene062808

ATGGAAGTTGTGAGAACAAGTGTGCTTTCCAAGCGATGGGAGTCATTATGGAAGAACACTCCTGACCTTAGTTTTGACCAAAGACAAATGTTGAAGTCTTTGATTGAAGAGTACATTCAAAATTCTCCACTTGATGACCGTCTTACAATGGCTATGCAACGCAAGGTAATTTTTATTGAAATCATGTAAAATAAGTTATGTGTTTTTTTACTATAAGATTTGTGTTTTAATTTATGCTTTATGAATAATTAGATTATTCAAATGGTTCACATTTTCATGAAGAGTCAACCAGGAAGGGTTGTAAAGAATCTAATTACATATTTTACCTTCCATTTTCTAAATGAAATGAATGAACTTTAATTCTAAACGTGTTTGAGATATGATATATATTGACGGAATTCATGCATGACAGGTAGCTCCTACAAATGAGGAGTATTTTGATGTAATTTCTGAAGCAGCCATGATGATTACATCAAATATTGACAATCATATTGGTCCCTTGAAGAGTTGTACCATCCGACACTTGCCTGAGAGTTGTGCAAGTGCGGATATTGTGGGGTGGTTAAGGAAAATACTGGAAAAAGGGGTGATTAAGGTTTCAATAGAACGTGAATCTTGTGATTATCTTAACGAAGGAATATTAGACTTGGATGCAATGCTTGCTGCATCGACCATTGATTTACCTTTTGAGGTTTTCTCCAATTTTAAGGTTCTTGAGTTGAAGAACTATCGTTTTAACACTACACCCTCCCCTGATTCCCAGCAGACTTTAAAAACACTTACTCTTAAGAAAGTGTGTATTATATCGAACACTTTTCATGATATTCTCTCATACTGTTCATCTCTTGAAAACCTTACCGTCGAGAATTGTGATTTCTTAACAGATGAGCTTAAGATTGTAAGTCCAAGTCTGAAATATATTAAAATTTGTAATATGAATGTGCTCAGGATTTTGGTTTCTGCATTTAATGTTGAAGTCATAGAGATTGACTCCATTATTTGTAGTCATGAAGATTTGGTCTTTGAGGCCCCAAAACTTCAAGTTCTTCGTGCTTACAATGATTTTCAAATTCTTGGAGAAATTTTATTTATTGATGGAAGAAAGCTTTTGACAGCAAGAGATATTATAGAAATTTTCGGTGGTATTCTGGTATGTCTTTCTTCACTTAGAAAATTGATTAACTTCAAGTTTCTGTTTTTTATTCAAGCATATAATACATTTCTTTTTAATTAGTACAGAACCATCTAATGTTCTAAATTTTTGTTGCATCTCCTTGGATATATGTACAACTTAACATATGGTCTATATTCAATTACATTGAATGTTGCAATGAACTGTAGATGCAAAAAAGTGAGTCAAGGATAAAGATTAAAGTTTAATATCCCTTAATGCAAGTATGATTCCTTTTTGAGCCATCACAAAAAATCACGCTTATTGGGAAATACTTCCTAGAAAAATATCATAGCTTATTATATGTGGACTAACCGAGTAATTGTGATGCGATGCTGAAAATTGTTGTTTATGGTATCACCACCTTACTGAACAGTAACCAAGTATGCCTACTACAAATATTGCTTAGAGTGAAATAATCCAATTTGATTTTGCTCCTTAGAAAAATGTCATCTAAAAAATAGACAAGTATATAGTAGTTTCTCACTTAACATTAATATCTTTATTATTTCTTAACATGTGTCATGAAGGCACGTGTTAGCACGACCCTTGTTTTGTAACTATCTTTCTTGATTGTCTTAGGGTCATCGAGGTTCAGGCATGGGAAATATATTTAAACATTTAGTTACTCTTTGCCTCAACTTTGAATTGAACAGTAGCAGAAATACCATAGCTCTTTCTTTTGCACTCAAGTCTTGCCCACAACTAATGAATCTTCAAATAAATAACCAGGTATGTTTGGGATTAATTTAGCCATATGTTTGCAACTATCTTTACTTCAGTTACATGTGTTCAATTATTCAAGTTTTAAAATGGCGTGTAATAGGAATTGTGGTTACAACAATAATATTTTTTATTTCATTGTGATCATGATTGTAGTCAAATTAGTCATATTTGACCATGATGTAACGATAAATCAATCATGATTTAAATTATGCTTAACTATATTTTTTTTTGACATTTTTCCTCTTAAACTCTAGGTCAAGTTGGATTATTATGATCAGAATGACAATGATAGTCTTCCTTACCCTGGTGGTTTGTTTTGGCTCAAAAGGGACCCCTGTAGGTGTGTTAGCCGTAGCCTAAAGTCTGTTTGTATAAAGGGATATAAAGGTGGAGAGTTTGAATTTGAGTTCGTTAAGTACTTAATATTAAATGGCGGAGTTATGAATAATATTACCATTTGGTTTTTGGATGAATGTTCATGGGATGAAGTTGTAGCAACTGCATGTTTGCGATCATATCCAAAACTCTCTCCTAAATTGTCAATTGATTTGAAACATGGGACCGAGTCTATACAGAAGTATGGTGGCAGCTTTGATGAATGGGTGAGAACCCTAAAATAA
Protein sequence

>MS.gene062808.t1

MEVVRTSVLSKRWESLWKNTPDLSFDQRQMLKSLIEEYIQNSPLDDRLTMAMQRKVAPTNEEYFDVISEAAMMITSNIDNHIGPLKSCTIRHLPESCASADIVGWLRKILEKGVIKVSIERESCDYLNEGILDLDAMLAASTIDLPFEVFSNFKVLELKNYRFNTTPSPDSQQTLKTLTLKKVCIISNTFHDILSYCSSLENLTVENCDFLTDELKIVSPSLKYIKICNMNVLRILVSAFNVEVIEIDSIICSHEDLVFEAPKLQVLRAYNDFQILGEILFIDGRKLLTARDIIEIFGGILGHRGSGMGNIFKHLVTLCLNFELNSSRNTIALSFALKSCPQLMNLQINNQVKLDYYDQNDNDSLPYPGGLFWLKRDPCRCVSRSLKSVCIKGYKGGEFEFEFVKYLILNGGVMNNITIWFLDECSWDEVVATACLRSYPKLSPKLSIDLKHGTESIQKYGGSFDEWVRTLK