AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062823

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062823.t1 MTR_2g071700 89.338 272 29 0 1 272 67 338 6.71e-174 490
MS.gene062823.t1 MTR_6g034190 55.180 444 178 4 1 432 66 500 6.07e-163 469
MS.gene062823.t1 MTR_2g070780 73.162 272 70 1 1 272 66 334 3.38e-132 385
MS.gene062823.t1 MTR_2g072240 48.380 432 166 5 1 430 59 435 1.32e-129 382
MS.gene062823.t1 MTR_2g071720 78.235 170 21 1 279 432 1 170 2.63e-91 274
MS.gene062823.t1 MTR_2g070660 89.516 124 13 0 8 131 44 167 4.51e-74 230
MS.gene062823.t1 MTR_2g070820 78.378 111 24 0 213 323 1 111 5.07e-54 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 80 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAATGAGCTCAGGATTT+TGG 0.213505 2.4:+24712428 MS.gene062823:CDS
CTTACCCCATAAGTTTGTTT+TGG 0.235274 2.4:+24714053 MS.gene062823:CDS
TTCAAATCAATTGACAATTT+AGG 0.238343 2.4:-24714286 None:intergenic
TAACTCAAGAACCTTAAAAT+TGG 0.249439 2.4:-24712193 None:intergenic
CCTAAAGTCTGTTTGTATAA+AGG 0.255416 2.4:+24714111 MS.gene062823:CDS
AGAAATTTGCGGTGGTATTC+TGG 0.260798 2.4:+24712623 MS.gene062823:CDS
GTTAAGTACTTAGTATTAAA+TGG 0.281525 2.4:+24714166 MS.gene062823:CDS
GGGAAATATATTTAAGCATT+TGG 0.282204 2.4:+24713627 MS.gene062823:CDS
TTTGAGCCAAAACAAACTTA+TGG 0.285224 2.4:-24714059 None:intergenic
GCATGAAGAACTTGAAGTTT+TGG 0.289235 2.4:-24712525 None:intergenic
TTTAAAGTCTGTTGGGAATC+AGG 0.294543 2.4:-24712246 None:intergenic
CTAAAGTCTGTTTGTATAAA+GGG 0.310052 2.4:+24714112 MS.gene062823:CDS
CCTTTATACAAACAGACTTT+AGG 0.311641 2.4:-24714111 None:intergenic
TACAATGAATTTCAAATGCT+TGG 0.324097 2.4:+24712549 MS.gene062823:CDS
TTGAGCCAAAACAAACTTAT+GGG 0.331764 2.4:-24714058 None:intergenic
TCTTAGGGTCATCGAGGTTC+AGG 0.358731 2.4:+24713601 MS.gene062823:intron
GACAATTTAGGAGAGAGTTT+TGG 0.359150 2.4:-24714274 None:intergenic
TTGTCAATTGATTTGAAACC+TGG 0.390272 2.4:+24714292 MS.gene062823:CDS
TATTTGTAGTCATGAAGATT+TGG 0.395241 2.4:+24712491 MS.gene062823:CDS
GGGGTGGTTGAGGAAATTAT+TGG 0.396243 2.4:+24712050 MS.gene062823:CDS
TCATGAAGATTTGGTCTTTG+AGG 0.413287 2.4:+24712500 MS.gene062823:CDS
AGTATTAAATGGCGGAGTTA+TGG 0.423482 2.4:+24714177 MS.gene062823:CDS
TTATGGATAATATTACCATT+TGG 0.432439 2.4:+24714194 MS.gene062823:CDS
CGGATGTTGTGGGGTGGTTG+AGG 0.432688 2.4:+24712040 MS.gene062823:CDS
TCGAATGGTACAACTCTTCA+AGG 0.460907 2.4:-24711992 None:intergenic
TCAAATATTGACAGTCATAT+CGG 0.467840 2.4:+24711967 MS.gene062823:CDS
TTAAAGTCTGTTGGGAATCA+GGG 0.478853 2.4:-24712245 None:intergenic
TCCGCACTTGCACAACTCTC+AGG 0.479520 2.4:-24712021 None:intergenic
CTGTACCAACTGCATGTTTG+CGG 0.483179 2.4:+24714245 MS.gene062823:CDS
TTTGAAGATTCATTAGTTGT+GGG 0.487958 2.4:-24713723 None:intergenic
TGTGCAAGTGCGGATGTTGT+GGG 0.492363 2.4:+24712030 MS.gene062823:CDS
TTGTGCAAGTGCGGATGTTG+TGG 0.492608 2.4:+24712029 MS.gene062823:CDS
GGTGTGGTAGCTTTGATGAA+TGG 0.494341 2.4:+24714337 MS.gene062823:CDS
ATTTGAAGATTCATTAGTTG+TGG 0.494505 2.4:-24713724 None:intergenic
CAAAACAAACTTATGGGGTA+AGG 0.495683 2.4:-24714052 None:intergenic
TGCGCTCCATAGCCATTGTA+AGG 0.504342 2.4:-24711634 None:intergenic
TGACCGCCTTACAATGGCTA+TGG 0.506948 2.4:+24711628 MS.gene062823:CDS
ATCTTCATGACTACAAATAA+CGG 0.514474 2.4:-24712487 None:intergenic
GTGTGGTAGCTTTGATGAAT+GGG 0.515371 2.4:+24714338 MS.gene062823:CDS
GGGAAGGTGTAGTGTGAAAA+CGG 0.525563 2.4:-24712224 None:intergenic
AACCTCAAAAGGTAAATCAA+TGG 0.528655 2.4:-24712166 None:intergenic
TTGCTAATGTGAATGAGCTC+AGG 0.530172 2.4:+24712421 MS.gene062823:CDS
AAATTGGAGAAAACCTCAAA+AGG 0.535849 2.4:-24712177 None:intergenic
AATCACAGTTCTCGACGGTA+AGG 0.541275 2.4:-24712347 None:intergenic
AGGGATATCATAGAAATTTG+CGG 0.543459 2.4:+24712612 MS.gene062823:CDS
GCCGAGTATATACAAAAGAA+TGG 0.545486 2.4:+24714316 MS.gene062823:CDS
AATATTTGATGTAATCATCA+AGG 0.552019 2.4:-24711953 None:intergenic
TACAAACAGACTTTAGGTTG+CGG 0.552706 2.4:-24714105 None:intergenic
GGTCATCGAGGTTCAGGCAT+GGG 0.558503 2.4:+24713607 MS.gene062823:CDS
TGTCAATTGATTTGAAACCT+GGG 0.558987 2.4:+24714293 MS.gene062823:CDS
ATTGTAAGGCGGTCAACAAG+TGG 0.579308 2.4:-24711620 None:intergenic
GTCAATTGATTTGAAACCTG+GGG 0.585160 2.4:+24714294 MS.gene062823:CDS
TACAATGGCTATGGAGCGCA+AGG 0.585834 2.4:+24711637 MS.gene062823:CDS
TGAGCCAAAACAAACTTATG+GGG 0.588293 2.4:-24714057 None:intergenic
TACTGTTTAATTCAAAGTCG+AGG 0.590219 2.4:-24713660 None:intergenic
ACTTGTTGACCGCCTTACAA+TGG 0.592987 2.4:+24711622 MS.gene062823:CDS
GAGTGCAAAAGAAAGAGCTA+TGG 0.595456 2.4:-24713692 None:intergenic
ACAGGTAGCTGCTAGAAATG+AGG 0.595849 2.4:+24711903 MS.gene062823:intron
GAATCTTCAAATAAATAACC+AGG 0.602091 2.4:+24713735 MS.gene062823:CDS
GAATCTTGTGATTATCGTAA+CGG 0.602581 2.4:+24712105 MS.gene062823:CDS
GTCTGTTGGGAATCAGGGGA+AGG 0.606465 2.4:-24712240 None:intergenic
GTTTGTATAAAGGGATACAA+TGG 0.615146 2.4:+24714121 MS.gene062823:CDS
GCCTGAGAGTTGTGCAAGTG+CGG 0.619755 2.4:+24712020 MS.gene062823:CDS
TAAAGTCTGTTGGGAATCAG+GGG 0.623672 2.4:-24712244 None:intergenic
GGGTCATCGAGGTTCAGGCA+TGG 0.625995 2.4:+24713606 MS.gene062823:intron
TGTATAAAGGGATACAATGG+TGG 0.627410 2.4:+24714124 MS.gene062823:CDS
TATGACCGCAAACATGCAGT+TGG 0.629676 2.4:-24714250 None:intergenic
TAAGAAATCACAGTTCTCGA+CGG 0.630854 2.4:-24712352 None:intergenic
ACTCTCAGGCAAGTGTCGAA+TGG 0.638228 2.4:-24712007 None:intergenic
CGAATGGTACAACTCTTCAA+GGG 0.641099 2.4:-24711991 None:intergenic
TAACGGAGGAATATCAGACA+TGG 0.656564 2.4:+24712122 MS.gene062823:CDS
GTGCAAGTGCGGATGTTGTG+GGG 0.661376 2.4:+24712031 MS.gene062823:CDS
TCTTGTGATTATCGTAACGG+AGG 0.669952 2.4:+24712108 MS.gene062823:CDS
GTATATACAAAAGAATGGTG+TGG 0.676742 2.4:+24714321 MS.gene062823:CDS
TTGCGGCTAACACACATACA+CGG 0.685148 2.4:-24714088 None:intergenic
TGATTGTCTTAGGGTCATCG+AGG 0.689894 2.4:+24713595 MS.gene062823:intron
AAGTACTTAGTATTAAATGG+CGG 0.690460 2.4:+24714169 MS.gene062823:CDS
GATATCATAGAAATTTGCGG+TGG 0.696039 2.4:+24712615 MS.gene062823:CDS
CAAGTGCGGATGTTGTGGGG+TGG 0.700014 2.4:+24712034 MS.gene062823:CDS
GCTCCATAGCCATTGTAAGG+CGG 0.718204 2.4:-24711631 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGATAGTTATAAAAAA+AGG - chr2.4:24713575-24713594 None:intergenic 10.0%
!! AAAAGATAGTTATAAAAAAA+GGG - chr2.4:24713574-24713593 None:intergenic 10.0%
!! ATTAATTTATAGTTATATCA+TGG - chr2.4:24713929-24713948 None:intergenic 10.0%
!! AACCGATAAATATATATAAA+CGG + chr2.4:24713302-24713321 MS.gene062823:intron 15.0%
!! AACCGTTTATATATATTTAT+CGG - chr2.4:24713307-24713326 None:intergenic 15.0%
!! ATTCATTTCATTCAAAAAAA+TGG - chr2.4:24711826-24711845 None:intergenic 15.0%
!! GAATAATTAGATTAATCAAA+TGG + chr2.4:24711736-24711755 MS.gene062823:intron 15.0%
!!! AATTTTATTTCTCAAAAGAT+TGG + chr2.4:24713472-24713491 MS.gene062823:intron 15.0%
!!! ATTTTAATATATTTCAGACT+TGG - chr2.4:24712402-24712421 None:intergenic 15.0%
!!! CAATTATTCAAGTTTTAAAA+TGG + chr2.4:24713818-24713837 MS.gene062823:intron 15.0%
!!! TATTTTTTTGTAGTTAATAG+AGG + chr2.4:24712724-24712743 MS.gene062823:intron 15.0%
!!! TTTACATGACATTTTTTTTA+AGG - chr2.4:24713178-24713197 None:intergenic 15.0%
!! AATATTTGATGTAATCATCA+AGG - chr2.4:24711956-24711975 None:intergenic 20.0%
!! ACAAAAATTAGAACATTAGA+TGG - chr2.4:24712751-24712770 None:intergenic 20.0%
!! ACTAATTGAGTAATTATGAT+GGG + chr2.4:24712998-24713017 MS.gene062823:intron 20.0%
!! ATATCACAACTTATTATATG+TGG + chr2.4:24712975-24712994 MS.gene062823:intron 20.0%
!! GTTAAGTACTTAGTATTAAA+TGG + chr2.4:24714166-24714185 MS.gene062823:CDS 20.0%
!! TATATGTACAACTTAACATA+TGG + chr2.4:24712792-24712811 MS.gene062823:intron 20.0%
!! TTATGGATAATATTACCATT+TGG + chr2.4:24714194-24714213 MS.gene062823:CDS 20.0%
!! TTCAAATCAATTGACAATTT+AGG - chr2.4:24714289-24714308 None:intergenic 20.0%
!!! ATAAGTTGTGATATTTTTCT+AGG - chr2.4:24712969-24712988 None:intergenic 20.0%
!!! ATGATGAAACTTTTTGTTTA+TGG + chr2.4:24713021-24713040 MS.gene062823:intron 20.0%
!!! ATTTATGTCAATTTGTTGTA+TGG + chr2.4:24713376-24713395 MS.gene062823:intron 20.0%
!!! TAAGCTTTAATATCTCTTAA+TGG + chr2.4:24712887-24712906 MS.gene062823:intron 20.0%
!!! TAAGTTGTGATATTTTTCTA+GGG - chr2.4:24712968-24712987 None:intergenic 20.0%
!!! TAATATTACCATTTGGTTTT+TGG + chr2.4:24714201-24714220 MS.gene062823:CDS 20.0%
!!! TATCTTTTTTGATTGTCTTA+GGG + chr2.4:24713586-24713605 MS.gene062823:intron 20.0%
!!! TTTATGTCAATTTGTTGTAT+GGG + chr2.4:24713377-24713396 MS.gene062823:intron 20.0%
! AAGTACTTAGTATTAAATGG+CGG + chr2.4:24714169-24714188 MS.gene062823:CDS 25.0%
! ATAGCAATGTTACATTATGA+GGG - chr2.4:24713345-24713364 None:intergenic 25.0%
! ATCTTCATGACTACAAATAA+CGG - chr2.4:24712490-24712509 None:intergenic 25.0%
! ATTTGAAGATTCATTAGTTG+TGG - chr2.4:24713727-24713746 None:intergenic 25.0%
! CAAAAGTGGAAATTATGTAT+TGG + chr2.4:24713440-24713459 MS.gene062823:intron 25.0%
! CTAAAGTCTGTTTGTATAAA+GGG + chr2.4:24714112-24714131 MS.gene062823:CDS 25.0%
! GAAAGATAGTTGCAAATATA+TGG - chr2.4:24713781-24713800 None:intergenic 25.0%
! GAATCTTCAAATAAATAACC+AGG + chr2.4:24713735-24713754 MS.gene062823:CDS 25.0%
! GACTAATTGAGTAATTATGA+TGG + chr2.4:24712997-24713016 MS.gene062823:intron 25.0%
! GAGGAAATTATTGGAAAAAT+GGG + chr2.4:24712059-24712078 MS.gene062823:CDS 25.0%
! GGGAAATATATTTAAGCATT+TGG + chr2.4:24713627-24713646 MS.gene062823:CDS 25.0%
! TAACTCAAGAACCTTAAAAT+TGG - chr2.4:24712196-24712215 None:intergenic 25.0%
! TACAATGAATTTCAAATGCT+TGG + chr2.4:24712549-24712568 MS.gene062823:CDS 25.0%
! TATAGCAATGTTACATTATG+AGG - chr2.4:24713346-24713365 None:intergenic 25.0%
! TATTTGTAGTCATGAAGATT+TGG + chr2.4:24712491-24712510 MS.gene062823:CDS 25.0%
! TCAAATATTGACAGTCATAT+CGG + chr2.4:24711967-24711986 MS.gene062823:CDS 25.0%
! TGAGATATGATACATATTGA+TGG + chr2.4:24711868-24711887 MS.gene062823:intron 25.0%
! TGAGGAAATTATTGGAAAAA+TGG + chr2.4:24712058-24712077 MS.gene062823:CDS 25.0%
! TTTAAGGAGCAAAATCAAAT+TGG - chr2.4:24713162-24713181 None:intergenic 25.0%
! TTTGAAGATTCATTAGTTGT+GGG - chr2.4:24713726-24713745 None:intergenic 25.0%
!! ACCATTCTTTTGTATATACT+CGG - chr2.4:24714320-24714339 None:intergenic 25.0%
!! ATGGTATCTATTTTATGCTT+AGG + chr2.4:24713040-24713059 MS.gene062823:intron 25.0%
!! CAATACATAATTTCCACTTT+TGG - chr2.4:24713442-24713461 None:intergenic 25.0%
!! TCAAATGGTTCATATTTTCA+TGG + chr2.4:24711751-24711770 MS.gene062823:intron 25.0%
!! TGGTATCTATTTTATGCTTA+GGG + chr2.4:24713041-24713060 MS.gene062823:intron 25.0%
!!! AAGTGTTTTTAAAGTCTGTT+GGG - chr2.4:24712256-24712275 None:intergenic 25.0%
!!! ATTTTTTGTGATGTCTCAAA+AGG - chr2.4:24712924-24712943 None:intergenic 25.0%
!!! CTATCTTTTTTGATTGTCTT+AGG + chr2.4:24713585-24713604 MS.gene062823:intron 25.0%
!!! TAAGTGTTTTTAAAGTCTGT+TGG - chr2.4:24712257-24712276 None:intergenic 25.0%
!!! TGAATGAATTTTAATCCCAA+AGG + chr2.4:24711841-24711860 MS.gene062823:intron 25.0%
!!! TTTTTTGTGATGTCTCAAAA+GGG - chr2.4:24712923-24712942 None:intergenic 25.0%
AAATTGGAGAAAACCTCAAA+AGG - chr2.4:24712180-24712199 None:intergenic 30.0%
AACCTCAAAAGGTAAATCAA+TGG - chr2.4:24712169-24712188 None:intergenic 30.0%
AATAAATAACCAGGTATGCT+TGG + chr2.4:24713744-24713763 MS.gene062823:intron 30.0%
AATTGAACACATGTAACTGA+AGG - chr2.4:24713803-24713822 None:intergenic 30.0%
ACAAAAAATTCACGCGTATT+GGG + chr2.4:24712935-24712954 MS.gene062823:intron 30.0%
AGGGATATCATAGAAATTTG+CGG + chr2.4:24712612-24712631 MS.gene062823:CDS 30.0%
ATAAATAACCAGGTATGCTT+GGG + chr2.4:24713745-24713764 MS.gene062823:intron 30.0%
ATCATATCTCAAATGCCTTT+GGG - chr2.4:24711859-24711878 None:intergenic 30.0%
ATCCTTAACATATGTCCTAA+GGG - chr2.4:24713418-24713437 None:intergenic 30.0%
ATTGGAAAAATGGGTGATTA+AGG + chr2.4:24712068-24712087 MS.gene062823:CDS 30.0%
CCTAAAGTCTGTTTGTATAA+AGG + chr2.4:24714111-24714130 MS.gene062823:CDS 30.0%
CCTTTATACAAACAGACTTT+AGG - chr2.4:24714114-24714133 None:intergenic 30.0%
GAATCTTGTGATTATCGTAA+CGG + chr2.4:24712105-24712124 MS.gene062823:CDS 30.0%
GCTTTCATTACTATTCAGTA+AGG - chr2.4:24713102-24713121 None:intergenic 30.0%
GTATATACAAAAGAATGGTG+TGG + chr2.4:24714321-24714340 MS.gene062823:CDS 30.0%
GTTTGTATAAAGGGATACAA+TGG + chr2.4:24714121-24714140 MS.gene062823:CDS 30.0%
TAAGCCATATTAGCAGTATA+GGG - chr2.4:24713127-24713146 None:intergenic 30.0%
TACTGTTTAATTCAAAGTCG+AGG - chr2.4:24713663-24713682 None:intergenic 30.0%
TATCATATCTCAAATGCCTT+TGG - chr2.4:24711860-24711879 None:intergenic 30.0%
TTCATTACTATTCAGTAAGG+TGG - chr2.4:24713099-24713118 None:intergenic 30.0%
TTGAGCCAAAACAAACTTAT+GGG - chr2.4:24714061-24714080 None:intergenic 30.0%
TTTGAGCCAAAACAAACTTA+TGG - chr2.4:24714062-24714081 None:intergenic 30.0%
! AAAGAAAGCTTTTGACAACA+AGG + chr2.4:24712592-24712611 MS.gene062823:CDS 30.0%
! AAGAAAGCTTTTGACAACAA+GGG + chr2.4:24712593-24712612 MS.gene062823:CDS 30.0%
! ATGAAGAACTTGAAGTTTTG+GGG - chr2.4:24712526-24712545 None:intergenic 30.0%
! CATGAAGAACTTGAAGTTTT+GGG - chr2.4:24712527-24712546 None:intergenic 30.0%
! TGAGGTTTTCTCCAATTTTA+AGG + chr2.4:24712182-24712201 MS.gene062823:CDS 30.0%
!! TGTCAATTGATTTGAAACCT+GGG + chr2.4:24714293-24714312 MS.gene062823:CDS 30.0%
!! TTGTCAATTGATTTGAAACC+TGG + chr2.4:24714292-24714311 MS.gene062823:CDS 30.0%
!!! AACCATTGATTTACCTTTTG+AGG + chr2.4:24712164-24712183 MS.gene062823:CDS 30.0%
!!! AAGTTTGTTTTGGCTCAAAA+GGG + chr2.4:24714063-24714082 MS.gene062823:CDS 30.0%
!!! CATTTTTTCCCTTATACTCT+AGG + chr2.4:24713988-24714007 MS.gene062823:intron 30.0%
!!! TAAGTTTGTTTTGGCTCAAA+AGG + chr2.4:24714062-24714081 MS.gene062823:CDS 30.0%
AAATTAATCCCAAGCATACC+TGG - chr2.4:24713756-24713775 None:intergenic 35.0%
AACCTTGACCTAGAGTATAA+GGG - chr2.4:24713999-24714018 None:intergenic 35.0%
ATGGTGTGTAAGATGAATTG+TGG + chr2.4:24713837-24713856 MS.gene062823:intron 35.0%
ATTCTTAACATGTGCCATGA+AGG + chr2.4:24713533-24713552 MS.gene062823:intron 35.0%
CAAAACAAACTTATGGGGTA+AGG - chr2.4:24714055-24714074 None:intergenic 35.0%
CACAAAAAATTCACGCGTAT+TGG + chr2.4:24712934-24712953 MS.gene062823:intron 35.0%
CATCCTTAACATATGTCCTA+AGG - chr2.4:24713419-24713438 None:intergenic 35.0%
CTAAGCCATATTAGCAGTAT+AGG - chr2.4:24713128-24713147 None:intergenic 35.0%
GATATCATAGAAATTTGCGG+TGG + chr2.4:24712615-24712634 MS.gene062823:CDS 35.0%
GCATTCATCCAAAAACCAAA+TGG - chr2.4:24714212-24714231 None:intergenic 35.0%
GCCGAGTATATACAAAAGAA+TGG + chr2.4:24714316-24714335 MS.gene062823:CDS 35.0%
TAAGAAATCACAGTTCTCGA+CGG - chr2.4:24712355-24712374 None:intergenic 35.0%
TAATGTTAACATGTGCCCTT+AGG + chr2.4:24713400-24713419 MS.gene062823:intron 35.0%
TATGTTAAGGATGCCAAAAG+TGG + chr2.4:24713426-24713445 MS.gene062823:intron 35.0%
TGAGCCAAAACAAACTTATG+GGG - chr2.4:24714060-24714079 None:intergenic 35.0%
TGTATAAAGGGATACAATGG+TGG + chr2.4:24714124-24714143 MS.gene062823:CDS 35.0%
TTAAAGTCTGTTGGGAATCA+GGG - chr2.4:24712248-24712267 None:intergenic 35.0%
! AGTATTAAATGGCGGAGTTA+TGG + chr2.4:24714177-24714196 MS.gene062823:CDS 35.0%
! GACAATTTAGGAGAGAGTTT+TGG - chr2.4:24714277-24714296 None:intergenic 35.0%
! GCATGAAGAACTTGAAGTTT+TGG - chr2.4:24712528-24712547 None:intergenic 35.0%
! TCATGAAGATTTGGTCTTTG+AGG + chr2.4:24712500-24712519 MS.gene062823:CDS 35.0%
! TTTAAAGTCTGTTGGGAATC+AGG - chr2.4:24712249-24712268 None:intergenic 35.0%
!! CTTACCCCATAAGTTTGTTT+TGG + chr2.4:24714053-24714072 MS.gene062823:CDS 35.0%
!! GTCAATTGATTTGAAACCTG+GGG + chr2.4:24714294-24714313 MS.gene062823:CDS 35.0%
!! GTTTTCAAGAGATGAACAGT+AGG - chr2.4:24712328-24712347 None:intergenic 35.0%
!! GTTTTTGGATGAATGCTCAT+GGG + chr2.4:24714216-24714235 MS.gene062823:CDS 35.0%
!! TACAAACAGACTTTAGGTTG+CGG - chr2.4:24714108-24714127 None:intergenic 35.0%
AGAAATTTGCGGTGGTATTC+TGG + chr2.4:24712623-24712642 MS.gene062823:CDS 40.0%
AGATGCAAACAAGTGAGTCA+AGG + chr2.4:24712856-24712875 MS.gene062823:intron 40.0%
CAACCTTGACCTAGAGTATA+AGG - chr2.4:24714000-24714019 None:intergenic 40.0%
CATGCTTATATGTGCCTTCA+TGG - chr2.4:24713550-24713569 None:intergenic 40.0%
CGAATGGTACAACTCTTCAA+GGG - chr2.4:24711994-24712013 None:intergenic 40.0%
GACTCACTTGTTTGCATCTA+CGG - chr2.4:24712855-24712874 None:intergenic 40.0%
GAGTGCAAAAGAAAGAGCTA+TGG - chr2.4:24713695-24713714 None:intergenic 40.0%
GCTTCCCTATACTGCTAATA+TGG + chr2.4:24713120-24713139 MS.gene062823:intron 40.0%
GTGTGTAAGATGAATTGTGG+TGG + chr2.4:24713840-24713859 MS.gene062823:intron 40.0%
TAAAGTCTGTTGGGAATCAG+GGG - chr2.4:24712247-24712266 None:intergenic 40.0%
TAACGGAGGAATATCAGACA+TGG + chr2.4:24712122-24712141 MS.gene062823:CDS 40.0%
TCGAATGGTACAACTCTTCA+AGG - chr2.4:24711995-24712014 None:intergenic 40.0%
TCTTGTGATTATCGTAACGG+AGG + chr2.4:24712108-24712127 MS.gene062823:CDS 40.0%
TGATGGAATTCATGCATGAC+AGG + chr2.4:24711885-24711904 MS.gene062823:intron 40.0%
TGCCCTTAGGACATATGTTA+AGG + chr2.4:24713413-24713432 MS.gene062823:intron 40.0%
TTCCCTTATACTCTAGGTCA+AGG + chr2.4:24713994-24714013 MS.gene062823:intron 40.0%
TTGCTAATGTGAATGAGCTC+AGG + chr2.4:24712421-24712440 MS.gene062823:CDS 40.0%
! TAAGGTGGTGATGACATACT+TGG - chr2.4:24713084-24713103 None:intergenic 40.0%
! TGTGAATGAGCTCAGGATTT+TGG + chr2.4:24712428-24712447 MS.gene062823:CDS 40.0%
!! GAGCGCAAGGTAATTTGTTA+TGG + chr2.4:24711650-24711669 MS.gene062823:intron 40.0%
!! GGTTTTTGGATGAATGCTCA+TGG + chr2.4:24714215-24714234 MS.gene062823:CDS 40.0%
!! GTGTGGTAGCTTTGATGAAT+GGG + chr2.4:24714338-24714357 MS.gene062823:CDS 40.0%
AATCACAGTTCTCGACGGTA+AGG - chr2.4:24712350-24712369 None:intergenic 45.0%
ACAGGTAGCTGCTAGAAATG+AGG + chr2.4:24711903-24711922 MS.gene062823:intron 45.0%
ATTGTAAGGCGGTCAACAAG+TGG - chr2.4:24711623-24711642 None:intergenic 45.0%
CTGTACCAACTGCATGTTTG+CGG + chr2.4:24714245-24714264 MS.gene062823:CDS 45.0%
GGGGTGGTTGAGGAAATTAT+TGG + chr2.4:24712050-24712069 MS.gene062823:CDS 45.0%
TATGACCGCAAACATGCAGT+TGG - chr2.4:24714253-24714272 None:intergenic 45.0%
TGATTGTCTTAGGGTCATCG+AGG + chr2.4:24713595-24713614 MS.gene062823:intron 45.0%
TTGCGGCTAACACACATACA+CGG - chr2.4:24714091-24714110 None:intergenic 45.0%
! ACTTGTTGACCGCCTTACAA+TGG + chr2.4:24711622-24711641 MS.gene062823:CDS 45.0%
! ATTTTCATGGAGAGTCAGCC+AGG + chr2.4:24711764-24711783 MS.gene062823:intron 45.0%
! GGGAAGGTGTAGTGTGAAAA+CGG - chr2.4:24712227-24712246 None:intergenic 45.0%
! TTTTGTATATACTCGGCCCC+AGG - chr2.4:24714313-24714332 None:intergenic 45.0%
!! GGTGTGGTAGCTTTGATGAA+TGG + chr2.4:24714337-24714356 MS.gene062823:CDS 45.0%
AGATTCTCTACAGCCCTTCC+TGG - chr2.4:24711785-24711804 None:intergenic 50.0%
GCTCCATAGCCATTGTAAGG+CGG - chr2.4:24711634-24711653 None:intergenic 50.0%
TACAATGGCTATGGAGCGCA+AGG + chr2.4:24711637-24711656 MS.gene062823:CDS 50.0%
TCTTAGGGTCATCGAGGTTC+AGG + chr2.4:24713601-24713620 MS.gene062823:intron 50.0%
TGACCGCCTTACAATGGCTA+TGG + chr2.4:24711628-24711647 MS.gene062823:CDS 50.0%
TGCGCTCCATAGCCATTGTA+AGG - chr2.4:24711637-24711656 None:intergenic 50.0%
TGTGCAAGTGCGGATGTTGT+GGG + chr2.4:24712030-24712049 MS.gene062823:CDS 50.0%
TTGTGCAAGTGCGGATGTTG+TGG + chr2.4:24712029-24712048 MS.gene062823:CDS 50.0%
!! ACTCTCAGGCAAGTGTCGAA+TGG - chr2.4:24712010-24712029 None:intergenic 50.0%
CATGGAGAGTCAGCCAGGAA+GGG + chr2.4:24711769-24711788 MS.gene062823:intron 55.0%
GCCTGAGAGTTGTGCAAGTG+CGG + chr2.4:24712020-24712039 MS.gene062823:CDS 55.0%
GGTCATCGAGGTTCAGGCAT+GGG + chr2.4:24713607-24713626 MS.gene062823:CDS 55.0%
GTCTGTTGGGAATCAGGGGA+AGG - chr2.4:24712243-24712262 None:intergenic 55.0%
GTGCAAGTGCGGATGTTGTG+GGG + chr2.4:24712031-24712050 MS.gene062823:CDS 55.0%
TCATGGAGAGTCAGCCAGGA+AGG + chr2.4:24711768-24711787 MS.gene062823:intron 55.0%
TCCGCACTTGCACAACTCTC+AGG - chr2.4:24712024-24712043 None:intergenic 55.0%
! TGCTTAGGGTGAGCACTGTC+TGG + chr2.4:24713055-24713074 MS.gene062823:intron 55.0%
CAAGTGCGGATGTTGTGGGG+TGG + chr2.4:24712034-24712053 MS.gene062823:CDS 60.0%
CGGATGTTGTGGGGTGGTTG+AGG + chr2.4:24712040-24712059 MS.gene062823:CDS 60.0%
GGGTCATCGAGGTTCAGGCA+TGG + chr2.4:24713606-24713625 MS.gene062823:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 24711581 24714363 24711581 ID=MS.gene062823
chr2.4 mRNA 24711581 24714363 24711581 ID=MS.gene062823.t1;Parent=MS.gene062823
chr2.4 exon 24711581 24711658 24711581 ID=MS.gene062823.t1.exon1;Parent=MS.gene062823.t1
chr2.4 CDS 24711581 24711658 24711581 ID=cds.MS.gene062823.t1;Parent=MS.gene062823.t1
chr2.4 exon 24711907 24712644 24711907 ID=MS.gene062823.t1.exon2;Parent=MS.gene062823.t1
chr2.4 CDS 24711907 24712644 24711907 ID=cds.MS.gene062823.t1;Parent=MS.gene062823.t1
chr2.4 exon 24713607 24713756 24713607 ID=MS.gene062823.t1.exon3;Parent=MS.gene062823.t1
chr2.4 CDS 24713607 24713756 24713607 ID=cds.MS.gene062823.t1;Parent=MS.gene062823.t1
chr2.4 exon 24714010 24714363 24714010 ID=MS.gene062823.t1.exon4;Parent=MS.gene062823.t1
chr2.4 CDS 24714010 24714363 24714010 ID=cds.MS.gene062823.t1;Parent=MS.gene062823.t1
Gene Sequence

>MS.gene062823

ATGTTGAAGTCTTTGATTGAAGATTACATTCAAAATTCTCCACTTGTTGACCGCCTTACAATGGCTATGGAGCGCAAGGTAATTTGTTATGGAAATCATGTAAAATAATTTATGTGTTTTTTACTATATGATTTATGTTTTAATTTATGCTTTATGAATAATTAGATTAATCAAATGGTTCATATTTTCATGGAGAGTCAGCCAGGAAGGGCTGTAGAGAATCTAATTACATATTTTAGCTTCCATTTTTTTGAATGAAATGAATGAATTTTAATCCCAAAGGCATTTGAGATATGATACATATTGATGGAATTCATGCATGACAGGTAGCTGCTAGAAATGAGGAATATTTTGATGTAATTGCTGAAGCAGCCTTGATGATTACATCAAATATTGACAGTCATATCGGTCCCTTGAAGAGTTGTACCATTCGACACTTGCCTGAGAGTTGTGCAAGTGCGGATGTTGTGGGGTGGTTGAGGAAATTATTGGAAAAATGGGTGATTAAGGTTTCAATAGAACGTGAATCTTGTGATTATCGTAACGGAGGAATATCAGACATGGATGTAAAGCTTGCTGCATCAACCATTGATTTACCTTTTGAGGTTTTCTCCAATTTTAAGGTTCTTGAGTTAAAGAACTACCGTTTTCACACTACACCTTCCCCTGATTCCCAACAGACTTTAAAAACACTTACTCTTAACAAAGTGCGTATTATATCGAACACTTTTCACGATATTCTCTCCTACTGTTCATCTCTTGAAAACCTTACCGTCGAGAACTGTGATTTCTTAAGAGATGAGCTTAAGATTGTAAGTCCAAGTCTGAAATATATTAAAATTGCTAATGTGAATGAGCTCAGGATTTTGGTTTCTGCATTTAATGTTGAAGTCATAGAGATTGACTCCGTTATTTGTAGTCATGAAGATTTGGTCTTTGAGGCCCCAAAACTTCAAGTTCTTCATGCTTACAATGAATTTCAAATGCTTGGAGAAATTGTATTTATTGATGAAAGAAAGCTTTTGACAACAAGGGATATCATAGAAATTTGCGGTGGTATTCTGGTATGTCTCTCTTCAAGTCTTTCTTCACTTGAAAAATTGATTAACTTCAATTTTCTGTTTTTTATTCAAGCGTATAATATATTTTTTTGTAGTTAATAGAGGTCCATCTAATGTTCTAATTTTTGTTGCATCTAAATTATCTTTAGATATATGTACAACTTAACATATGGTCTATATATTCAATTACATTGAATGTTACAATGATATCCGTAGATGCAAACAAGTGAGTCAAGGATAATGATTAAGCTTTAATATCTCTTAATGGAAGTATGATTCCCTTTTGAGACATCACAAAAAATTCACGCGTATTGGGAAATATTCCCTAGAAAAATATCACAACTTATTATATGTGGACTAATTGAGTAATTATGATGGGATGATGAAACTTTTTGTTTATGGTATCTATTTTATGCTTAGGGTGAGCACTGTCTGGTAACCAAGTATGTCATCACCACCTTACTGAATAGTAATGAAAGCTTCCCTATACTGCTAATATGGCTTAGAGCGAAATAATCCAATTTGATTTTGCTCCTTAAAAAAAATGTCATGTAAAAAATATACAAGTATATATTATTTTCTTACTTGACATTAGTATATTTACTATTCATGTATATATATAATTTAGATTGCTAAGTATCGTTAATAAAATTTTACAACAACCGATAAATATATATAAACGGTTAAAGCTTTGACTGCACCCTCATAATGTAACATTGCTATATTTTATGAACATTTATGTCAATTTGTTGTATGGGTAATGTTAACATGTGCCCTTAGGACATATGTTAAGGATGCCAAAAGTGGAAATTATGTATTGGAAGTGATGCAATTTTATTTCTCAAAAGATTGGTATTTAATTTTTCTAATTAAATATTTTCTATTTGAAGAATTCTTAACATGTGCCATGAAGGCACATATAAGCATGACCCTTTTTTTATAACTATCTTTTTTGATTGTCTTAGGGTCATCGAGGTTCAGGCATGGGAAATATATTTAAGCATTTGGTTACTCTTTGCCTCGACTTTGAATTAAACAGTAGCAGAAATACCATAGCTCTTTCTTTTGCACTCAAGTCTTGCCCACAACTAATGAATCTTCAAATAAATAACCAGGTATGCTTGGGATTAATTTAGCCATATATTTGCAACTATCTTTCCTTCAGTTACATGTGTTCAATTATTCAAGTTTTAAAATGGTGTGTAAGATGAATTGTGGTGGCAGCGATAATATTTTTTTTTATTTAAGTGTGATCGTGATTGTAGTCAAATTAGTCATATATAACCATGATATAACTATAAATTAATCTTGATTTAAATTATGCTTCATTATATTATTTTTTTTGACATTTTTTCCCTTATACTCTAGGTCAAGGTTGATTACTATGATCAGAATGACAATGATTCTCTTCCTTACCCCATAAGTTTGTTTTGGCTCAAAAGGGATCCGTGTATGTGTGTTAGCCGCAACCTAAAGTCTGTTTGTATAAAGGGATACAATGGTGGAGAGTTTGAATTTGAGTTTGTTAAGTACTTAGTATTAAATGGCGGAGTTATGGATAATATTACCATTTGGTTTTTGGATGAATGCTCATGGGATGAAGCTGTACCAACTGCATGTTTGCGGTCATATCCAAAACTCTCTCCTAAATTGTCAATTGATTTGAAACCTGGGGCCGAGTATATACAAAAGAATGGTGTGGTAGCTTTGATGAATGGGTGA
Protein sequence

>MS.gene062823.t1

MLKSLIEDYIQNSPLVDRLTMAMERKVAARNEEYFDVIAEAALMITSNIDSHIGPLKSCTIRHLPESCASADVVGWLRKLLEKWVIKVSIERESCDYRNGGISDMDVKLAASTIDLPFEVFSNFKVLELKNYRFHTTPSPDSQQTLKTLTLNKVRIISNTFHDILSYCSSLENLTVENCDFLRDELKIVSPSLKYIKIANVNELRILVSAFNVEVIEIDSVICSHEDLVFEAPKLQVLHAYNEFQMLGEIVFIDERKLLTTRDIIEICGGILGHRGSGMGNIFKHLVTLCLDFELNSSRNTIALSFALKSCPQLMNLQINNQVKVDYYDQNDNDSLPYPISLFWLKRDPCMCVSRNLKSVCIKGYNGGEFEFEFVKYLVLNGGVMDNITIWFLDECSWDEAVPTACLRSYPKLSPKLSIDLKPGAEYIQKNGVVALMNG