AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063458

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063458.t1 MTR_2g070780 93.255 341 23 0 6 346 1 341 0.0 650
MS.gene063458.t1 MTR_2g071700 72.485 338 89 2 6 339 1 338 1.39e-163 464
MS.gene063458.t1 MTR_6g034190 55.430 442 173 4 6 432 1 433 2.36e-162 467
MS.gene063458.t1 MTR_2g072240 46.897 435 158 8 6 432 1 370 1.06e-121 361
MS.gene063458.t1 MTR_2g070820 90.991 111 10 0 280 390 1 111 8.27e-66 206
MS.gene063458.t1 MTR_2g070660 57.360 197 50 3 6 198 1 167 7.84e-62 198
MS.gene063458.t1 MTR_2g071720 71.154 104 14 1 346 433 1 104 2.54e-43 150
MS.gene063458.t1 MTR_2g070810 95.745 47 2 0 6 52 49 95 2.86e-23 94.0
MS.gene063458.t1 MTR_8g014180 54.545 55 25 0 6 60 1 55 3.16e-12 61.6
MS.gene063458.t1 MTR_7g095350 27.554 323 196 12 15 328 14 307 6.18e-12 67.8
MS.gene063458.t1 MTR_7g095350 27.554 323 196 12 15 328 6 299 6.39e-12 67.8
MS.gene063458.t1 MTR_6g013240 24.092 303 197 10 10 302 60 339 6.69e-12 67.8
MS.gene063458.t1 MTR_7g095350 27.554 323 196 12 15 328 6 299 7.12e-12 67.4
MS.gene063458.t1 MTR_7g095350 27.554 323 196 12 15 328 14 307 7.20e-12 67.4
MS.gene063458.t1 MTR_7g095350 27.554 323 196 12 15 328 6 299 7.57e-12 67.4
MS.gene063458.t1 MTR_2g082860 56.000 50 22 0 21 70 26 75 7.94e-12 62.8
MS.gene063458.t1 MTR_4g024770 40.845 71 42 0 2 72 4 74 9.61e-12 60.8
MS.gene063458.t1 MTR_7g007370 50.820 61 30 0 6 66 1 61 9.67e-12 66.2
MS.gene063458.t1 MTR_6g013380 24.579 297 191 10 16 302 61 334 1.47e-11 66.6
MS.gene063458.t1 MTR_2g047858 27.636 275 152 10 18 286 111 344 5.01e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063458.t1 AT1G80960 31.310 313 154 12 22 318 11 278 1.29e-21 97.1
MS.gene063458.t1 AT1G80960 31.310 313 154 12 22 318 50 317 1.34e-21 96.7
MS.gene063458.t1 AT1G80960 31.310 313 154 12 22 318 50 317 1.59e-21 97.1
MS.gene063458.t1 AT1G80960 31.310 313 154 12 22 318 50 317 2.13e-21 96.7
MS.gene063458.t1 AT1G80960 31.310 313 154 12 22 318 50 317 2.13e-21 96.7
MS.gene063458.t1 AT1G80960 31.310 313 154 12 22 318 50 317 2.13e-21 96.7
MS.gene063458.t1 AT1G67390 26.333 300 183 9 7 302 27 292 3.52e-16 80.9
MS.gene063458.t1 AT3G26922 27.241 290 159 8 21 302 13 258 4.55e-15 76.3
MS.gene063458.t1 AT3G26922 27.241 290 159 8 21 302 13 258 4.55e-15 76.3
MS.gene063458.t1 AT3G59190 24.784 464 257 21 21 433 11 433 9.48e-15 76.6
MS.gene063458.t1 AT5G41830 27.083 240 137 7 11 243 2 210 2.91e-13 72.0
MS.gene063458.t1 AT3G59190 24.500 400 223 18 21 384 11 367 2.61e-12 68.6
MS.gene063458.t1 AT1G78760 23.202 431 239 16 11 410 5 374 4.45e-12 68.2
MS.gene063458.t1 AT3G60790 27.124 306 177 14 7 306 40 305 1.31e-11 66.6
MS.gene063458.t1 AT5G62970 22.779 439 247 17 29 432 10 391 2.86e-11 65.5
MS.gene063458.t1 AT5G41840 25.769 260 146 9 22 266 14 241 5.54e-11 65.1

Find 89 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAATCTGTTGAGGATTA+AGG 0.153196 2.3:+25092230 None:intergenic
CCTCACACTCTAGGTCAATT+TGG 0.235008 2.3:-25090817 MS.gene063458:intron
CTTACCCTGGAAGTTTGTTT+TGG 0.254096 2.3:-25090761 MS.gene063458:CDS
CAAAATGTCTGAAGAAATAA+TGG 0.265099 2.3:-25093176 None:intergenic
TGTGAACGAGCGTAGGATTT+TGG 0.266869 2.3:-25092047 MS.gene063458:CDS
TGAAATTTGTGGTGGCATTT+TGG 0.276341 2.3:-25091852 MS.gene063458:intron
CCTTGATACAAACATTCTTT+AGG 0.277807 2.3:+25090703 None:intergenic
GATTCTTGTGATTATCGTTA+TGG 0.307940 2.3:-25092370 MS.gene063458:CDS
GCACGTAGAACCCGAAGTTT+TGG 0.308366 2.3:+25091950 None:intergenic
CAAAATCTGTTGAGGATTAA+GGG 0.329839 2.3:+25092231 None:intergenic
TTCAGCCAAAACAAACTTCC+AGG 0.338704 2.3:+25090756 None:intergenic
TTTGTCTTTCGTCAAAATTA+AGG 0.347473 2.3:+25092984 None:intergenic
TACAATAATGTTCAAATGCT+TGG 0.353231 2.3:-25091926 MS.gene063458:CDS
AACTTCAATCTTGCAGTGAC+TGG 0.362551 2.3:-25092917 MS.gene063458:CDS
CTTAGAGGCCCCAAAACTTC+GGG 0.365917 2.3:-25091960 MS.gene063458:CDS
TTGTGCAAGTGGGGATGTTG+TGG 0.396215 2.3:-25092446 MS.gene063458:CDS
AGGGATATTATTGAAATTTG+TGG 0.398913 2.3:-25091863 MS.gene063458:CDS
TGTGCAAGTGGGGATGTTGT+GGG 0.402090 2.3:-25092445 MS.gene063458:CDS
CAACTCAAGAACCTTAAAAC+TGG 0.403099 2.3:+25092282 None:intergenic
TTATTTGAAGATTCATTAGT+TGG 0.403722 2.3:+25090930 None:intergenic
TGCAAGATTGAAGTTTAGTC+TGG 0.413386 2.3:+25092925 None:intergenic
TCATGAAGATCTGATCTTAG+AGG 0.415542 2.3:-25091975 MS.gene063458:CDS
TATTTGAAGATTCATTAGTT+GGG 0.418765 2.3:+25090931 None:intergenic
TCAAATATTGACAATCATAT+TGG 0.420752 2.3:-25092508 MS.gene063458:CDS
CCTAAAGAATGTTTGTATCA+AGG 0.437189 2.3:-25090703 MS.gene063458:CDS
TACAAACATTCTTTAGGTTG+TGG 0.449660 2.3:+25090709 None:intergenic
CCACAATGATTCCCATCGCT+TGG 0.455748 2.3:+25093021 None:intergenic
GTTCTCACGACTTCCGTGTA+AGG 0.459091 2.3:+25093055 None:intergenic
GGGATGTTGTGGGGTGGATG+AGG 0.463671 2.3:-25092435 MS.gene063458:CDS
GCATTCCTTTCAATCATGTC+TGG 0.464346 2.3:+25092340 None:intergenic
CTAAAGAATGTTTGTATCAA+GGG 0.467863 2.3:-25090702 MS.gene063458:CDS
TCTAATGCTACAACTCTTCA+AGG 0.468328 2.3:+25092483 None:intergenic
ATATAAGCTAGAAGTGCATC+AGG 0.471803 2.3:+25093091 None:intergenic
TCCCCACTTGCACAACTCTC+AGG 0.473005 2.3:+25092454 None:intergenic
AGGGACACAAATCAAAGTTA+TGG 0.481343 2.3:-25093143 MS.gene063458:CDS
ACGACTTCCGTGTAAGGAAG+TGG 0.493916 2.3:+25093061 None:intergenic
AAACTGGAGAAAACTTCAAA+AGG 0.495741 2.3:+25092298 None:intergenic
CATTTCTCCACTTCCTTACA+CGG 0.495992 2.3:-25093068 MS.gene063458:CDS
GTGCAAGTGGGGATGTTGTG+GGG 0.497345 2.3:-25092444 MS.gene063458:CDS
TCTTAGAGGCCCCAAAACTT+CGG 0.498004 2.3:-25091961 MS.gene063458:CDS
ATCTTCATGATTACAAATAA+CGG 0.505209 2.3:+25091988 None:intergenic
CAAGTGGGGATGTTGTGGGG+TGG 0.508240 2.3:-25092441 MS.gene063458:CDS
GAAATAATGGGTGAAGGAGA+AGG 0.509757 2.3:-25093163 MS.gene063458:CDS
CAAAACAAACTTCCAGGGTA+AGG 0.518602 2.3:+25090762 None:intergenic
CTTTCGTCAAAATTAAGGCT+AGG 0.519973 2.3:+25092989 None:intergenic
TTAAGAAATCACATTTCTCA+AGG 0.522132 2.3:+25092122 None:intergenic
AAAATGTCTGAAGAAATAAT+GGG 0.529206 2.3:-25093175 MS.gene063458:CDS
TCAATTCAAAATCGAGGCAA+AGG 0.529641 2.3:+25091002 None:intergenic
ATTTGAAGATTCATTAGTTG+GGG 0.541423 2.3:+25090932 None:intergenic
AAATAATGGGTGAAGGAGAA+GGG 0.545422 2.3:-25093162 MS.gene063458:CDS
TGCAGTGACTGGGCAACGTA+AGG 0.547546 2.3:-25092906 MS.gene063458:intron
CAATTCAAAATCGAGGCAAA+GGG 0.555813 2.3:+25091003 None:intergenic
CAAGTGTGCTCTCCAAGCGA+TGG 0.562082 2.3:-25093033 MS.gene063458:CDS
AACTTCAAAAGGTAAATCAA+TGG 0.562125 2.3:+25092309 None:intergenic
GATATTATTGAAATTTGTGG+TGG 0.564526 2.3:-25091860 MS.gene063458:CDS
TACCGTTCAATTCAAAATCG+AGG 0.564943 2.3:+25090996 None:intergenic
ACTTCAATCTTGCAGTGACT+GGG 0.567133 2.3:-25092916 MS.gene063458:CDS
ATGTGATTTCTTAACAGATG+AGG 0.570169 2.3:-25092113 MS.gene063458:CDS
TCAGCCAAAACAAACTTCCA+GGG 0.571326 2.3:+25090757 None:intergenic
TCTTGTGATTATCGTTATGG+AGG 0.571564 2.3:-25092367 MS.gene063458:CDS
TTTGGTTTCTGCATTTAACA+TGG 0.582670 2.3:-25092029 MS.gene063458:CDS
GAATACCAGACATGATTGAA+AGG 0.585171 2.3:-25092345 MS.gene063458:CDS
TCTGAAGAAATAATGGGTGA+AGG 0.588283 2.3:-25093169 MS.gene063458:CDS
AAGTGTGCTCTCCAAGCGAT+GGG 0.588507 2.3:-25093032 MS.gene063458:CDS
TTTGTGATGTGAACGAGCGT+AGG 0.592653 2.3:-25092054 MS.gene063458:CDS
GGTCATCGAGGTTCTAGCAT+GGG 0.596942 2.3:-25091049 MS.gene063458:CDS
GACACAAATCAAAGTTATGG+AGG 0.600709 2.3:-25093140 MS.gene063458:CDS
GAATCTTCAAATAAATAACC+AGG 0.602091 2.3:-25090921 MS.gene063458:intron
TACTATGATCAAAATGACAA+TGG 0.603700 2.3:-25090792 MS.gene063458:CDS
CCAAGCGATGGGAATCATTG+TGG 0.604799 2.3:-25093021 MS.gene063458:CDS
GTTTGTATCAAGGGATACAA+AGG 0.605257 2.3:-25090693 None:intergenic
AATATTTGATGTAACCATCA+TGG 0.606995 2.3:+25092522 None:intergenic
ACAGGTAGATCTTGAAAGTG+AGG 0.613723 2.3:-25092563 MS.gene063458:intron
GTTTGCTGAAGCAGCCATGA+TGG 0.615425 2.3:-25092536 MS.gene063458:CDS
TTGTGGCTGACACACCTACA+CGG 0.616160 2.3:+25090726 None:intergenic
AATGGCTGTCTTCCTTACCC+TGG 0.616901 2.3:-25090774 MS.gene063458:CDS
ATCTGTTGAGGATTAAGGGA+GGG 0.619665 2.3:+25092235 None:intergenic
AGAGTATTCAAAATCTGTTG+AGG 0.620364 2.3:+25092223 None:intergenic
TGCCTGAGAGTTGTGCAAGT+GGG 0.620485 2.3:-25092456 MS.gene063458:CDS
GCCTGAGAGTTGTGCAAGTG+GGG 0.621594 2.3:-25092455 MS.gene063458:CDS
CAAGAGATGAACAATGAGAG+AGG 0.622832 2.3:+25092155 None:intergenic
GGGTCATCGAGGTTCTAGCA+TGG 0.629061 2.3:-25091050 MS.gene063458:CDS
TGTGGCTGACACACCTACAC+GGG 0.629776 2.3:+25090727 None:intergenic
CTAATGCTACAACTCTTCAA+GGG 0.636034 2.3:+25092484 None:intergenic
GGCTGAAAAGAGACCCGTGT+AGG 0.671792 2.3:-25090740 MS.gene063458:CDS
TTGCCTGAGAGTTGTGCAAG+TGG 0.675075 2.3:-25092457 MS.gene063458:CDS
TGATTGTCTTAGGGTCATCG+AGG 0.687412 2.3:-25091061 MS.gene063458:intron
AATCTGTTGAGGATTAAGGG+AGG 0.688199 2.3:+25092234 None:intergenic
TTTGAAGATTCATTAGTTGG+GGG 0.726871 2.3:+25090933 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCTTTATATAAATTCTT+TGG + chr2.3:25092703-25092722 None:intergenic 10.0%
!! GTGTATATATAATTTATATA+TGG - chr2.3:25092202-25092221 MS.gene063458:CDS 10.0%
!! TCAAATATGATATATATTAT+TGG - chr2.3:25091268-25091287 MS.gene063458:intron 10.0%
!! ATGATATCTATTTAATACTT+AGG - chr2.3:25092459-25092478 MS.gene063458:CDS 15.0%
!! TAGATGAACTTTAATTATAA+AGG - chr2.3:25091241-25091260 MS.gene063458:intron 15.0%
!!! CACAAATTTTAATATATTTG+AGG + chr2.3:25091798-25091817 None:intergenic 15.0%
!!! CATCTATTTTATTAGAAAAA+TGG + chr2.3:25091227-25091246 None:intergenic 15.0%
!!! TATATAAAGAATTGAAGTTT+TGG - chr2.3:25092710-25092729 MS.gene063458:intron 15.0%
!!! TGTTTTTTACTATAAACTTT+AGG - chr2.3:25091017-25091036 MS.gene063458:CDS 15.0%
!! AAATAATAGATACTCATGAT+AGG + chr2.3:25092644-25092663 None:intergenic 20.0%
!! AGTTCATAGCTTATTATATA+TGG - chr2.3:25092394-25092413 MS.gene063458:CDS 20.0%
!! ATCAAACAGTTCATATTTAA+TGG - chr2.3:25091152-25091171 MS.gene063458:intron 20.0%
!! ATCTTCATGATTACAAATAA+CGG + chr2.3:25091881-25091900 None:intergenic 20.0%
!! CATTTCTTTATAGTTAATAG+AGG - chr2.3:25092099-25092118 MS.gene063458:CDS 20.0%
!! TAATCAATATTTGTAGCATA+AGG + chr2.3:25092546-25092565 None:intergenic 20.0%
!! TATTTGAAGATTCATTAGTT+GGG + chr2.3:25092938-25092957 None:intergenic 20.0%
!! TCAAATATTGACAATCATAT+TGG - chr2.3:25091358-25091377 MS.gene063458:intron 20.0%
!! TTATTTGAAGATTCATTAGT+TGG + chr2.3:25092939-25092958 None:intergenic 20.0%
!!! AGGTTTTATTACTATTCAAT+AGG + chr2.3:25092522-25092541 None:intergenic 20.0%
!!! ATTTTAATATATTTGAGGCT+TGG + chr2.3:25091793-25091812 None:intergenic 20.0%
!!! GGTTTTATTACTATTCAATA+GGG + chr2.3:25092521-25092540 None:intergenic 20.0%
!!! TATAGTAATACTACATTTTG+AGG + chr2.3:25092742-25092761 None:intergenic 20.0%
!!! TATATTTTCTGATTGTCTTA+GGG - chr2.3:25092796-25092815 MS.gene063458:intron 20.0%
!!! TTATATTTTCTGATTGTCTT+AGG - chr2.3:25092795-25092814 MS.gene063458:intron 20.0%
! AACTTCAAAAGGTAAATCAA+TGG + chr2.3:25091560-25091579 None:intergenic 25.0%
! AATATTTGATGTAACCATCA+TGG + chr2.3:25091347-25091366 None:intergenic 25.0%
! ACTAATTGAGTATTTGTGAT+AGG - chr2.3:25092417-25092436 MS.gene063458:CDS 25.0%
! AGGGATATTATTGAAATTTG+TGG - chr2.3:25092003-25092022 MS.gene063458:CDS 25.0%
! ATTTGAAGATTCATTAGTTG+GGG + chr2.3:25092937-25092956 None:intergenic 25.0%
! CTAAAGAATGTTTGTATCAA+GGG - chr2.3:25093164-25093183 MS.gene063458:CDS 25.0%
! GAATCTTCAAATAAATAACC+AGG - chr2.3:25092945-25092964 MS.gene063458:CDS 25.0%
! GATATTATTGAAATTTGTGG+TGG - chr2.3:25092006-25092025 MS.gene063458:CDS 25.0%
! TACAATAATGTTCAAATGCT+TGG - chr2.3:25091940-25091959 MS.gene063458:CDS 25.0%
! TACTATGATCAAAATGACAA+TGG - chr2.3:25093074-25093093 MS.gene063458:CDS 25.0%
! TATTTAATGGAGAATGAACT+AGG - chr2.3:25091165-25091184 MS.gene063458:intron 25.0%
! TTAAGAAATCACATTTCTCA+AGG + chr2.3:25091747-25091766 None:intergenic 25.0%
! TTTGTCTTTCGTCAAAATTA+AGG + chr2.3:25090885-25090904 None:intergenic 25.0%
!! TAAGCTATGAACTTTTTGTA+TGG + chr2.3:25092387-25092406 None:intergenic 25.0%
!!! GGACAAAATGTTTTTATAGA+TGG - chr2.3:25091961-25091980 MS.gene063458:CDS 25.0%
!!! GGGAAGTATCTTTAAAAATT+TGG - chr2.3:25092837-25092856 MS.gene063458:intron 25.0%
!!! TTTTTTGTGAAGATTAACCA+TGG + chr2.3:25091106-25091125 None:intergenic 25.0%
AAACTGGAGAAAACTTCAAA+AGG + chr2.3:25091571-25091590 None:intergenic 30.0%
ATGTGATTTCTTAACAGATG+AGG - chr2.3:25091753-25091772 MS.gene063458:intron 30.0%
CAAAATCTGTTGAGGATTAA+GGG + chr2.3:25091638-25091657 None:intergenic 30.0%
CAATATTTGTAGCATAAGGA+AGG + chr2.3:25092542-25092561 None:intergenic 30.0%
CCTAAAGAATGTTTGTATCA+AGG - chr2.3:25093163-25093182 MS.gene063458:CDS 30.0%
CCTTGATACAAACATTCTTT+AGG + chr2.3:25093166-25093185 None:intergenic 30.0%
GATTCTTGTGATTATCGTTA+TGG - chr2.3:25091496-25091515 MS.gene063458:intron 30.0%
TATTGGACTTTATGCATAAC+AGG - chr2.3:25091285-25091304 MS.gene063458:intron 30.0%
TCAAAATCTGTTGAGGATTA+AGG + chr2.3:25091639-25091658 None:intergenic 30.0%
TTAGAAAACTCTCTATGCAA+TGG - chr2.3:25092569-25092588 MS.gene063458:intron 30.0%
TTTGAAGATTCATTAGTTGG+GGG + chr2.3:25092936-25092955 None:intergenic 30.0%
! AGAGTATTCAAAATCTGTTG+AGG + chr2.3:25091646-25091665 None:intergenic 30.0%
! TGAAGTTTTCTCCAGTTTTA+AGG - chr2.3:25091573-25091592 MS.gene063458:intron 30.0%
! TTTGGTTTCTGCATTTAACA+TGG - chr2.3:25091837-25091856 MS.gene063458:intron 30.0%
!! TACAAACATTCTTTAGGTTG+TGG + chr2.3:25093160-25093179 None:intergenic 30.0%
!!! TTTTTTTTGTTGATCGTGTC+TGG - chr2.3:25091058-25091077 MS.gene063458:intron 30.0%
ACTCGAAAAAGAGGTGATTA+AGG - chr2.3:25091459-25091478 MS.gene063458:intron 35.0%
AGGGAATCATACATGCATTA+AGG + chr2.3:25092324-25092343 None:intergenic 35.0%
AGGGACACAAATCAAAGTTA+TGG - chr2.3:25090723-25090742 MS.gene063458:CDS 35.0%
CAACTCAAGAACCTTAAAAC+TGG + chr2.3:25091587-25091606 None:intergenic 35.0%
CAATTCAAAATCGAGGCAAA+GGG + chr2.3:25092866-25092885 None:intergenic 35.0%
CACAGAAAATCACGCTTATT+GGG - chr2.3:25092354-25092373 MS.gene063458:CDS 35.0%
CTAATGCTACAACTCTTCAA+GGG + chr2.3:25091385-25091404 None:intergenic 35.0%
CTTTCGTCAAAATTAAGGCT+AGG + chr2.3:25090880-25090899 None:intergenic 35.0%
GAATACCAGACATGATTGAA+AGG - chr2.3:25091521-25091540 MS.gene063458:intron 35.0%
GACACAAATCAAAGTTATGG+AGG - chr2.3:25090726-25090745 MS.gene063458:CDS 35.0%
GAGGAAATTACTCGAAAAAG+AGG - chr2.3:25091450-25091469 MS.gene063458:intron 35.0%
TACCGTTCAATTCAAAATCG+AGG + chr2.3:25092873-25092892 None:intergenic 35.0%
TACTTAGGATGAACACAGAT+TGG - chr2.3:25092474-25092493 MS.gene063458:CDS 35.0%
TCAATTCAAAATCGAGGCAA+AGG + chr2.3:25092867-25092886 None:intergenic 35.0%
TCACAGAAAATCACGCTTAT+TGG - chr2.3:25092353-25092372 MS.gene063458:CDS 35.0%
TCATGAAGATCTGATCTTAG+AGG - chr2.3:25091891-25091910 MS.gene063458:CDS 35.0%
TCTAATGCTACAACTCTTCA+AGG + chr2.3:25091386-25091405 None:intergenic 35.0%
TCTTGTGATTATCGTTATGG+AGG - chr2.3:25091499-25091518 MS.gene063458:intron 35.0%
TGCAAGATTGAAGTTTAGTC+TGG + chr2.3:25090944-25090963 None:intergenic 35.0%
TGCTGCATTACAATTCAACT+AGG - chr2.3:25092149-25092168 MS.gene063458:CDS 35.0%
TTCCAAGTGAAGTATAGTAG+AGG + chr2.3:25092049-25092068 None:intergenic 35.0%
TTCCTCTACTATACTTCACT+TGG - chr2.3:25092044-25092063 MS.gene063458:CDS 35.0%
! ATAAGCGTGATTTTCTGTGA+TGG + chr2.3:25092353-25092372 None:intergenic 35.0%
! ATATAAGCTAGAAGTGCATC+AGG + chr2.3:25090778-25090797 None:intergenic 35.0%
! ATGCAAGCTTTTGACAACAA+GGG - chr2.3:25091984-25092003 MS.gene063458:CDS 35.0%
! ATTTTCTGTGATGGCTCAAA+AGG + chr2.3:25092344-25092363 None:intergenic 35.0%
! TGAAATTTGTGGTGGCATTT+TGG - chr2.3:25092014-25092033 MS.gene063458:CDS 35.0%
! TGCCTCGATTTTGAATTGAA+CGG - chr2.3:25092868-25092887 MS.gene063458:intron 35.0%
! TTTTCTGTGATGGCTCAAAA+GGG + chr2.3:25092343-25092362 None:intergenic 35.0%
!! AAATAATGGGTGAAGGAGAA+GGG - chr2.3:25090704-25090723 MS.gene063458:CDS 35.0%
!! ACTATTCAATAGGGTGTTGA+TGG + chr2.3:25092512-25092531 None:intergenic 35.0%
!! TCTGAAGAAATAATGGGTGA+AGG - chr2.3:25090697-25090716 MS.gene063458:CDS 35.0%
AACTTCAATCTTGCAGTGAC+TGG - chr2.3:25090949-25090968 MS.gene063458:CDS 40.0%
AATCTGTTGAGGATTAAGGG+AGG + chr2.3:25091635-25091654 None:intergenic 40.0%
ACAGGTAGATCTTGAAAGTG+AGG - chr2.3:25091303-25091322 MS.gene063458:intron 40.0%
ACATTTAACCCTCACACTCT+AGG - chr2.3:25093040-25093059 MS.gene063458:CDS 40.0%
ACTTCAATCTTGCAGTGACT+GGG - chr2.3:25090950-25090969 MS.gene063458:CDS 40.0%
ATAACCAGGTATGCTTGCTT+AGG - chr2.3:25092959-25092978 MS.gene063458:CDS 40.0%
ATCTGTTGAGGATTAAGGGA+GGG + chr2.3:25091634-25091653 None:intergenic 40.0%
CAAAACAAACTTCCAGGGTA+AGG + chr2.3:25093107-25093126 None:intergenic 40.0%
CAAATTGACCTAGAGTGTGA+GGG + chr2.3:25093051-25093070 None:intergenic 40.0%
CAAGAGATGAACAATGAGAG+AGG + chr2.3:25091714-25091733 None:intergenic 40.0%
CATTTCTCCACTTCCTTACA+CGG - chr2.3:25090798-25090817 MS.gene063458:CDS 40.0%
CTATATCTCTAGACAAGCCA+TGG - chr2.3:25091086-25091105 MS.gene063458:intron 40.0%
GCATTCCTTTCAATCATGTC+TGG + chr2.3:25091529-25091548 None:intergenic 40.0%
GGCTTGTCTAGAGATATAGA+GGG + chr2.3:25091085-25091104 None:intergenic 40.0%
TAATCCTAAGCAAGCATACC+TGG + chr2.3:25092966-25092985 None:intergenic 40.0%
TCAGCCAAAACAAACTTCCA+GGG + chr2.3:25093112-25093131 None:intergenic 40.0%
TTCAGCCAAAACAAACTTCC+AGG + chr2.3:25093113-25093132 None:intergenic 40.0%
! GATGCAAGCTTTTGACAACA+AGG - chr2.3:25091983-25092002 MS.gene063458:CDS 40.0%
! TGGCTTGTCTAGAGATATAG+AGG + chr2.3:25091086-25091105 None:intergenic 40.0%
!! CTTACCCTGGAAGTTTGTTT+TGG - chr2.3:25093105-25093124 MS.gene063458:CDS 40.0%
!! GAAATAATGGGTGAAGGAGA+AGG - chr2.3:25090703-25090722 MS.gene063458:CDS 40.0%
AAGATTCATTAGTTGGGGGC+AGG + chr2.3:25092932-25092951 None:intergenic 45.0%
CCAAATTGACCTAGAGTGTG+AGG + chr2.3:25093052-25093071 None:intergenic 45.0%
CCTCACACTCTAGGTCAATT+TGG - chr2.3:25093049-25093068 MS.gene063458:CDS 45.0%
TCTTAGAGGCCCCAAAACTT+CGG - chr2.3:25091905-25091924 MS.gene063458:CDS 45.0%
TGATTGTCTTAGGGTCATCG+AGG - chr2.3:25092805-25092824 MS.gene063458:intron 45.0%
TTTGTGATGTGAACGAGCGT+AGG - chr2.3:25091812-25091831 MS.gene063458:intron 45.0%
! ACGTAGAACCCGAAGTTTTG+GGG + chr2.3:25091917-25091936 None:intergenic 45.0%
! CACGTAGAACCCGAAGTTTT+GGG + chr2.3:25091918-25091937 None:intergenic 45.0%
! TGTGAACGAGCGTAGGATTT+TGG - chr2.3:25091819-25091838 MS.gene063458:intron 45.0%
AAGTGTGCTCTCCAAGCGAT+GGG - chr2.3:25090834-25090853 MS.gene063458:intron 50.0%
AATGGCTGTCTTCCTTACCC+TGG - chr2.3:25093092-25093111 MS.gene063458:CDS 50.0%
ACGACTTCCGTGTAAGGAAG+TGG + chr2.3:25090808-25090827 None:intergenic 50.0%
CCAAGCGATGGGAATCATTG+TGG - chr2.3:25090845-25090864 MS.gene063458:intron 50.0%
CCACAATGATTCCCATCGCT+TGG + chr2.3:25090848-25090867 None:intergenic 50.0%
CTTAGAGGCCCCAAAACTTC+GGG - chr2.3:25091906-25091925 MS.gene063458:CDS 50.0%
GTTCTCACGACTTCCGTGTA+AGG + chr2.3:25090814-25090833 None:intergenic 50.0%
TGCCTGAGAGTTGTGCAAGT+GGG - chr2.3:25091410-25091429 MS.gene063458:intron 50.0%
TGTGCAAGTGGGGATGTTGT+GGG - chr2.3:25091421-25091440 MS.gene063458:intron 50.0%
TTGCCTGAGAGTTGTGCAAG+TGG - chr2.3:25091409-25091428 MS.gene063458:intron 50.0%
TTGTGCAAGTGGGGATGTTG+TGG - chr2.3:25091420-25091439 MS.gene063458:intron 50.0%
TTGTGGCTGACACACCTACA+CGG + chr2.3:25093143-25093162 None:intergenic 50.0%
! GCACGTAGAACCCGAAGTTT+TGG + chr2.3:25091919-25091938 None:intergenic 50.0%
! GGTCATCGAGGTTCTAGCAT+GGG - chr2.3:25092817-25092836 MS.gene063458:intron 50.0%
! GTTTGCTGAAGCAGCCATGA+TGG - chr2.3:25091330-25091349 MS.gene063458:intron 50.0%
!! TAGGGTGTTGATGGCGTACT+AGG + chr2.3:25092503-25092522 None:intergenic 50.0%
CAAGTGTGCTCTCCAAGCGA+TGG - chr2.3:25090833-25090852 MS.gene063458:intron 55.0%
GCCTGAGAGTTGTGCAAGTG+GGG - chr2.3:25091411-25091430 MS.gene063458:intron 55.0%
GGCTGAAAAGAGACCCGTGT+AGG - chr2.3:25093126-25093145 MS.gene063458:CDS 55.0%
GTGCAAGTGGGGATGTTGTG+GGG - chr2.3:25091422-25091441 MS.gene063458:intron 55.0%
TCCCCACTTGCACAACTCTC+AGG + chr2.3:25091415-25091434 None:intergenic 55.0%
TGCAGTGACTGGGCAACGTA+AGG - chr2.3:25090960-25090979 MS.gene063458:CDS 55.0%
TGTGGCTGACACACCTACAC+GGG + chr2.3:25093142-25093161 None:intergenic 55.0%
! GGGTCATCGAGGTTCTAGCA+TGG - chr2.3:25092816-25092835 MS.gene063458:intron 55.0%
CAAGTGGGGATGTTGTGGGG+TGG - chr2.3:25091425-25091444 MS.gene063458:intron 60.0%
GGGATGTTGTGGGGTGGATG+AGG - chr2.3:25091431-25091450 MS.gene063458:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 25090694 25093194 25090694 ID=MS.gene063458
chr2.3 mRNA 25090694 25093194 25090694 ID=MS.gene063458.t1;Parent=MS.gene063458
chr2.3 exon 25092907 25093194 25092907 ID=MS.gene063458.t1.exon1;Parent=MS.gene063458.t1
chr2.3 CDS 25092907 25093194 25092907 ID=cds.MS.gene063458.t1;Parent=MS.gene063458.t1
chr2.3 exon 25091853 25092581 25091853 ID=MS.gene063458.t1.exon2;Parent=MS.gene063458.t1
chr2.3 CDS 25091853 25092581 25091853 ID=cds.MS.gene063458.t1;Parent=MS.gene063458.t1
chr2.3 exon 25090922 25091071 25090922 ID=MS.gene063458.t1.exon3;Parent=MS.gene063458.t1
chr2.3 CDS 25090922 25091071 25090922 ID=cds.MS.gene063458.t1;Parent=MS.gene063458.t1
chr2.3 exon 25090694 25090826 25090694 ID=MS.gene063458.t1.exon4;Parent=MS.gene063458.t1
chr2.3 CDS 25090694 25090826 25090694 ID=cds.MS.gene063458.t1;Parent=MS.gene063458.t1
Gene Sequence

>MS.gene063458

ATGTCTGAAGAAATAATGGGTGAAGGAGAAGGGACACAAATCAAAGTTATGGAGGAAGAAGAAGATTTGATCAGCAATCTTCCTGATGCACTTCTAGCTTATATCATTTCTCCACTTCCTTACACGGAAGTCGTGAGAACAAGTGTGCTCTCCAAGCGATGGGAATCATTGTGGAAACACACTCCTAGCCTTAATTTTGACGAAAGACAAATGTTAAAGTCATTGATCAAAGAAAACAATCAAAATTCCAGACTAAACTTCAATCTTGCAGTGACTGGGCAACGTAAGGTACATTGTTATAAAAATTTATGTAAAATTTTATGTGTTTTTTACTATAAACTTTAGGTTTTAATTTATGATTGTTTTTTTTTTGTTGATCGTGTCTGGTCCCTCTATATCTCTAGACAAGCCATGGTTAATCTTCACAAAAAATTATAGATATCTTAATAATTAGATTAATCAAACAGTTCATATTTAATGGAGAATGAACTAGGAAAAATTAGAGAGAATATGATTACATATTTTAGCTTCCATTTTTCTAATAAAATAGATGAACTTTAATTATAAAGGCTTTTCAAATATGATATATATTATTGGACTTTATGCATAACAGGTAGATCTTGAAAGTGAGGAACTGTTTGCTGAAGCAGCCATGATGGTTACATCAAATATTGACAATCATATTGGTCCCTTGAAGAGTTGTAGCATTAGACACTTGCCTGAGAGTTGTGCAAGTGGGGATGTTGTGGGGTGGATGAGGAAATTACTCGAAAAAGAGGTGATTAAGGTTTCAATAGAACGTGATTCTTGTGATTATCGTTATGGAGGAATACCAGACATGATTGAAAGGAATGCTGCATCAACCATTGATTTACCTTTTGAAGTTTTCTCCAGTTTTAAGGTTCTTGAGTTGAAGAACTATCATTTTTTAACTACACCCTCCCTTAATCCTCAACAGATTTTGAATACTCTTACTCTTAACAAAGTGCATATTATATCTAACACTTTTCATGATATCCTCTCTCATTGTTCATCTCTTGAAAATCTTATCCTTGAGAAATGTGATTTCTTAACAGATGAGGTTAAGATTGTAAGTCCAAGCCTCAAATATATTAAAATTTGTGATGTGAACGAGCGTAGGATTTTGGTTTCTGCATTTAACATGGAAGTCATAGAGATTGACTCCGTTATTTGTAATCATGAAGATCTGATCTTAGAGGCCCCAAAACTTCGGGTTCTACGTGCTTACAATAATGTTCAAATGCTTGGACAAAATGTTTTTATAGATGGATGCAAGCTTTTGACAACAAGGGATATTATTGAAATTTGTGGTGGCATTTTGGTAAGTGTTTCCTCTACTATACTTCACTTGGAATGTCAGTTTTTTTGATTGATGCATATATTACATTTCTTTATAGTTAATAGAGGTGCATGTAATCTAATGTTTTAAATTTTTGCTGCATTACAATTCAACTAGGATATATATATATATATGTGTGTGTGTGTGTGTGTATATATAATTTATATATGGTTCATATTCAATTACAATTGAATATTACAATCATATGTACATATGCAATCATTAAGATTAATACGTGAGTCAATGATAATGATTAAACTTTAATATCCTTAATGCATGTATGATTCCCTTTTGAGCCATCACAGAAAATCACGCTTATTGGGCAATACTCCATACAAAAAGTTCATAGCTTATTATATATGGACTAATTGAGTATTTGTGATAGGATAATAAAACTTGTTGTTTATGATATCTATTTAATACTTAGGATGAACACAGATTGGTAACCTAGTACGCCATCAACACCCTATTGAATAGTAATAAAACCTTCCTTATGCTACAAATATTGATTAGAGTTAGAAAACTCTCTATGCAATGGCTTAGAAATATGTCATGTAAAAAATAGACAATTATATTTTAGTTTTTGACCTATCATGAGTATCTATTATTTAGATCGTTCAGTAATGCTAATTAAATTTTACAGCAACCAAAGAATTTATATAAAGAATTGAAGTTTTGGTTGTATCCTCAAAATGTAGTATTACTATAATTTATGAACATTTATGTCTATTTGTTTTATAATTATATTTTCTGATTGTCTTAGGGTCATCGAGGTTCTAGCATGGGAAGTATCTTTAAAAATTTGGTTACCCTTTGCCTCGATTTTGAATTGAACGGTAATAGAAATGTCATAGCTCTTTCTTTTGCACTCAAGTCCTGCCCCCAACTAATGAATCTTCAAATAAATAACCAGGTATGCTTGCTTAGGATTATTTTAGCTTGATCATGATATTGCAGTCATTATACTTAACTATATATTTTTTTTGACATTTAACCCTCACACTCTAGGTCAATTTGGATTACTATGATCAAAATGACAATGGCTGTCTTCCTTACCCTGGAAGTTTGTTTTGGCTGAAAAGAGACCCGTGTAGGTGTGTCAGCCACAACCTAAAGAATGTTTGTATCAAGGGATACAAAG
Protein sequence

>MS.gene063458.t1

MSEEIMGEGEGTQIKVMEEEEDLISNLPDALLAYIISPLPYTEVVRTSVLSKRWESLWKHTPSLNFDERQMLKSLIKENNQNSRLNFNLAVTGQRKVDLESEELFAEAAMMVTSNIDNHIGPLKSCSIRHLPESCASGDVVGWMRKLLEKEVIKVSIERDSCDYRYGGIPDMIERNAASTIDLPFEVFSSFKVLELKNYHFLTTPSLNPQQILNTLTLNKVHIISNTFHDILSHCSSLENLILEKCDFLTDEVKIVSPSLKYIKICDVNERRILVSAFNMEVIEIDSVICNHEDLILEAPKLRVLRAYNNVQMLGQNVFIDGCKLLTTRDIIEICGGILGHRGSSMGSIFKNLVTLCLDFELNGNRNVIALSFALKSCPQLMNLQINNQVNLDYYDQNDNGCLPYPGSLFWLKRDPCRCVSHNLKNVCIKGYK