Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063690.t1 | XP_003630882.1 | 93.1 | 290 | 17 | 3 | 1 | 290 | 188 | 474 | 1.20E-151 | 545.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063690.t1 | Q9C9T2 | 55.9 | 238 | 97 | 5 | 1 | 234 | 179 | 412 | 1.3e-69 | 264.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063690.t1 | G7L9S5 | 93.1 | 290 | 17 | 3 | 1 | 290 | 188 | 474 | 8.6e-152 | 545.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049409 | MS.gene063690 | 0.848276 | 6.80E-60 | -1.69E-46 |
| MS.gene050148 | MS.gene063690 | 0.81098 | 8.80E-51 | -1.69E-46 |
| MS.gene050149 | MS.gene063690 | 0.803845 | 2.87E-49 | -1.69E-46 |
| MS.gene050669 | MS.gene063690 | 0.80039 | 1.47E-48 | -1.69E-46 |
| MS.gene050961 | MS.gene063690 | 0.801184 | 1.01E-48 | -1.69E-46 |
| MS.gene05861 | MS.gene063690 | 0.933509 | 1.68E-95 | -1.69E-46 |
| MS.gene05862 | MS.gene063690 | 0.988125 | 8.33E-173 | -1.69E-46 |
| MS.gene05969 | MS.gene063690 | 0.936582 | 1.38E-97 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063690.t1 | MTR_8g104510 | 91.379 | 290 | 22 | 3 | 1 | 290 | 188 | 474 | 0.0 | 538 |
| MS.gene063690.t1 | MTR_8g104490 | 87.586 | 290 | 31 | 5 | 1 | 289 | 188 | 473 | 0.0 | 512 |
| MS.gene063690.t1 | MTR_8g104490 | 87.586 | 290 | 31 | 5 | 1 | 289 | 123 | 408 | 0.0 | 510 |
| MS.gene063690.t1 | MTR_6g083730 | 60.825 | 291 | 103 | 5 | 1 | 289 | 189 | 470 | 1.64e-121 | 356 |
| MS.gene063690.t1 | MTR_2g450080 | 44.622 | 251 | 122 | 5 | 1 | 235 | 189 | 438 | 1.05e-67 | 221 |
| MS.gene063690.t1 | MTR_2g011810 | 47.234 | 235 | 110 | 5 | 1 | 230 | 191 | 416 | 1.63e-65 | 216 |
| MS.gene063690.t1 | MTR_4g128600 | 52.239 | 201 | 92 | 2 | 1 | 198 | 188 | 387 | 6.21e-65 | 214 |
| MS.gene063690.t1 | MTR_4g090970 | 50.249 | 201 | 96 | 2 | 1 | 198 | 190 | 389 | 1.95e-64 | 213 |
| MS.gene063690.t1 | MTR_4g106810 | 60.000 | 140 | 55 | 1 | 1 | 140 | 176 | 314 | 2.72e-57 | 192 |
| MS.gene063690.t1 | MTR_6g083320 | 74.775 | 111 | 28 | 0 | 1 | 111 | 189 | 299 | 1.29e-55 | 185 |
| MS.gene063690.t1 | MTR_7g112000 | 34.109 | 258 | 157 | 7 | 1 | 255 | 176 | 423 | 2.10e-34 | 130 |
| MS.gene063690.t1 | MTR_7g111980 | 36.816 | 201 | 116 | 7 | 1 | 193 | 101 | 298 | 6.52e-27 | 109 |
| MS.gene063690.t1 | MTR_7g111980 | 36.318 | 201 | 117 | 7 | 1 | 193 | 184 | 381 | 9.49e-27 | 109 |
| MS.gene063690.t1 | MTR_7g111960 | 35.122 | 205 | 121 | 7 | 1 | 197 | 192 | 392 | 2.84e-24 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063690.t1 | AT1G73805 | 55.882 | 238 | 97 | 5 | 1 | 234 | 179 | 412 | 6.16e-87 | 267 |
| MS.gene063690.t1 | AT5G57580 | 52.239 | 201 | 92 | 2 | 1 | 198 | 188 | 387 | 2.06e-65 | 216 |
| MS.gene063690.t1 | AT4G25800 | 50.746 | 201 | 95 | 2 | 1 | 198 | 177 | 376 | 1.64e-64 | 213 |
| MS.gene063690.t1 | AT4G25800 | 50.746 | 201 | 95 | 2 | 1 | 198 | 177 | 376 | 1.64e-64 | 213 |
| MS.gene063690.t1 | AT2G18750 | 50.000 | 200 | 96 | 3 | 1 | 198 | 192 | 389 | 3.69e-62 | 207 |
| MS.gene063690.t1 | AT2G18750 | 50.000 | 200 | 96 | 3 | 1 | 198 | 192 | 389 | 3.69e-62 | 207 |
| MS.gene063690.t1 | AT2G18750 | 50.000 | 200 | 96 | 3 | 1 | 198 | 192 | 389 | 3.69e-62 | 207 |
| MS.gene063690.t1 | AT5G62570 | 47.783 | 203 | 100 | 3 | 1 | 199 | 163 | 363 | 9.61e-60 | 198 |
| MS.gene063690.t1 | AT5G62570 | 47.783 | 203 | 100 | 3 | 1 | 199 | 178 | 378 | 1.90e-59 | 197 |
| MS.gene063690.t1 | AT2G24300 | 48.529 | 204 | 101 | 2 | 1 | 201 | 136 | 338 | 7.35e-59 | 197 |
| MS.gene063690.t1 | AT5G62570 | 48.404 | 188 | 92 | 2 | 1 | 184 | 178 | 364 | 1.47e-58 | 192 |
| MS.gene063690.t1 | AT2G24300 | 48.529 | 204 | 101 | 2 | 1 | 201 | 183 | 385 | 1.71e-58 | 197 |
| MS.gene063690.t1 | AT2G24300 | 48.529 | 204 | 101 | 2 | 1 | 201 | 187 | 389 | 2.01e-58 | 197 |
| MS.gene063690.t1 | AT4G31000 | 46.262 | 214 | 107 | 3 | 1 | 211 | 187 | 395 | 8.53e-57 | 192 |
| MS.gene063690.t1 | AT4G31000 | 40.654 | 214 | 103 | 4 | 1 | 211 | 182 | 374 | 1.85e-44 | 158 |
| MS.gene063690.t1 | AT5G26920 | 39.409 | 203 | 108 | 7 | 1 | 199 | 177 | 368 | 4.25e-40 | 144 |
| MS.gene063690.t1 | AT5G62570 | 56.911 | 123 | 52 | 1 | 1 | 123 | 178 | 299 | 6.66e-40 | 141 |
| MS.gene063690.t1 | AT5G26920 | 39.409 | 203 | 108 | 7 | 1 | 199 | 177 | 368 | 2.21e-39 | 145 |
| MS.gene063690.t1 | AT5G26920 | 38.916 | 203 | 101 | 8 | 1 | 199 | 177 | 360 | 6.26e-37 | 138 |
Find 0 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAATGAATATATAGTT+AGG | - | 57340:1136-1155 | None:intergenic | 10.0% |
| !! | TATAAATGATGTATATTTAT+AGG | + | 57340:1364-1383 | MS.gene063690:intron | 10.0% |
| !! | TATACATCATTTATAATTAT+TGG | - | 57340:1359-1378 | None:intergenic | 10.0% |
| !! | AAATAACATTCTCAATTATA+TGG | + | 57340:1412-1431 | MS.gene063690:intron | 15.0% |
| !! | CATTATTAATGTTCAATAAA+TGG | + | 57340:1183-1202 | MS.gene063690:intron | 15.0% |
| !! | TATAAATTAGAATATCTTGA+AGG | - | 57340:513-532 | None:intergenic | 15.0% |
| !!! | TACTTTTGGTTAAATAATTT+TGG | - | 57340:1879-1898 | None:intergenic | 15.0% |
| !!! | TCTAAATTTCATTTTTCAAT+TGG | - | 57340:1907-1926 | None:intergenic | 15.0% |
| !!! | TTCTCTATGATTTTTTTTTT+TGG | + | 57340:591-610 | MS.gene063690:intron | 15.0% |
| !! | ACTAAAATAAAGGATCATAT+AGG | + | 57340:818-837 | MS.gene063690:intron | 20.0% |
| !! | ACTGTTAATTAATGATTAGT+TGG | + | 57340:746-765 | MS.gene063690:intron | 20.0% |
| !! | CTAAAATAAAGGATCATATA+GGG | + | 57340:819-838 | MS.gene063690:intron | 20.0% |
| !! | CTATTACTATAAGACTAAAA+AGG | - | 57340:849-868 | None:intergenic | 20.0% |
| !! | GCAAATTAAATGAACTATAT+AGG | - | 57340:1711-1730 | None:intergenic | 20.0% |
| !! | TTGGGTTATAACTAAAATAA+AGG | + | 57340:808-827 | MS.gene063690:intron | 20.0% |
| !!! | GCACAATGTATTTTTATATA+CGG | + | 57340:1282-1301 | MS.gene063690:intron | 20.0% |
| !!! | TAATTTATTTTACGGTTGTT+GGG | + | 57340:790-809 | MS.gene063690:intron | 20.0% |
| !!! | TATGATCAAAACAACTATTT+TGG | + | 57340:2038-2057 | MS.gene063690:CDS | 20.0% |
| !!! | TTAATTTATTTTACGGTTGT+TGG | + | 57340:789-808 | MS.gene063690:intron | 20.0% |
| !!! | TTCAATTGGAAATTTACTTT+TGG | - | 57340:1893-1912 | None:intergenic | 20.0% |
| !!! | TTTTGATCATATGTTATAAC+TGG | - | 57340:2029-2048 | None:intergenic | 20.0% |
| ! | AAAAACCAATGCATAGAAAA+AGG | - | 57340:1686-1705 | None:intergenic | 25.0% |
| ! | AACAACAATAATGCACAAAT+AGG | + | 57340:2098-2117 | MS.gene063690:CDS | 25.0% |
| ! | AATGAATATATAGTTAGGAG+AGG | - | 57340:1131-1150 | None:intergenic | 25.0% |
| ! | AATGAGAATCCAATATGTTA+GGG | + | 57340:422-441 | MS.gene063690:intron | 25.0% |
| ! | AGGGTTTATATAGATTAAGA+TGG | - | 57340:1111-1130 | None:intergenic | 25.0% |
| ! | ATGAATATATAGTTAGGAGA+GGG | - | 57340:1130-1149 | None:intergenic | 25.0% |
| ! | TAAAACTAACCCTAACATAT+TGG | - | 57340:434-453 | None:intergenic | 25.0% |
| ! | TAATGAGAATCCAATATGTT+AGG | + | 57340:421-440 | MS.gene063690:intron | 25.0% |
| ! | TTTATTGAATCTCACTATAC+AGG | + | 57340:1963-1982 | MS.gene063690:intron | 25.0% |
| ATCCTTAACAACAAAAGCTT+CGG | - | 57340:347-366 | None:intergenic | 30.0% | |
| TGTGATGCATTTAAATGAAG+AGG | - | 57340:643-662 | None:intergenic | 30.0% | |
| TTAGAGAAGATTGGTAAAGA+CGG | + | 57340:986-1005 | MS.gene063690:CDS | 30.0% | |
| TTCAAATGGATGGTAAATTG+AGG | - | 57340:1546-1565 | None:intergenic | 30.0% | |
| TTTGAATGCAATATGCCAAA+TGG | + | 57340:1560-1579 | MS.gene063690:CDS | 30.0% | |
| TTTGGCATATTGCATTCAAA+TGG | - | 57340:1560-1579 | None:intergenic | 30.0% | |
| ACAAAAAGTTGACCTCAGAA+GGG | + | 57340:1017-1036 | MS.gene063690:CDS | 35.0% | |
| ACGTATCCAGCTAGAATTAT+CGG | - | 57340:251-270 | None:intergenic | 35.0% | |
| AGCTAATTAAACTAGCTACC+TGG | + | 57340:667-686 | MS.gene063690:intron | 35.0% | |
| ATATGCTAGATGGAATGAGT+TGG | + | 57340:1762-1781 | MS.gene063690:CDS | 35.0% | |
| ATGGTTAGAGCTAACATCAA+TGG | + | 57340:1579-1598 | MS.gene063690:CDS | 35.0% | |
| ATTGTTTGCAAATTGCTCCA+TGG | - | 57340:1994-2013 | None:intergenic | 35.0% | |
| CAAACTTCATCCTTTAGACT+TGG | - | 57340:962-981 | None:intergenic | 35.0% | |
| CAAGTCTAAAGGATGAAGTT+TGG | + | 57340:960-979 | MS.gene063690:CDS | 35.0% | |
| CATGCAAAGACTTGTGTAAT+GGG | + | 57340:1495-1514 | MS.gene063690:CDS | 35.0% | |
| CGAGACATTAACAACACATT+GGG | + | 57340:1618-1637 | MS.gene063690:CDS | 35.0% | |
| GCATATTGCATTCAAATGGA+TGG | - | 57340:1556-1575 | None:intergenic | 35.0% | |
| TAGAAAAAGGTAGCAGTAGA+AGG | - | 57340:1673-1692 | None:intergenic | 35.0% | |
| ! | AACGTTGCTTTGTTAACTCA+AGG | + | 57340:1811-1830 | MS.gene063690:CDS | 35.0% |
| ! | CCAATGTGTTGTTAATGTCT+CGG | - | 57340:1620-1639 | None:intergenic | 35.0% |
| ! | TGCTACCTTTTTCTATGCAT+TGG | + | 57340:1678-1697 | MS.gene063690:intron | 35.0% |
| !! | TGATGTTAGCTCTAACCATT+TGG | - | 57340:1578-1597 | None:intergenic | 35.0% |
| !! | TGGTTTTGCTAGCAGATTTT+AGG | + | 57340:1432-1451 | MS.gene063690:intron | 35.0% |
| AACAAACTCTATGTCTACCG+CGG | + | 57340:1519-1538 | MS.gene063690:CDS | 40.0% | |
| AACTGTTGTAATCCCTTCTG+AGG | - | 57340:1032-1051 | None:intergenic | 40.0% | |
| AAGAAAACTCACATTCGCCA+CGG | - | 57340:372-391 | None:intergenic | 40.0% | |
| ACATCATCCACCAAGTCTAA+AGG | + | 57340:949-968 | MS.gene063690:CDS | 40.0% | |
| ACCACCAAAGCATATATACC+AGG | - | 57340:688-707 | None:intergenic | 40.0% | |
| ACCTGGTATATATGCTTTGG+TGG | + | 57340:684-703 | MS.gene063690:intron | 40.0% | |
| ACTTCATCCTTTAGACTTGG+TGG | - | 57340:959-978 | None:intergenic | 40.0% | |
| AGTTCACCGATAATTCTAGC+TGG | + | 57340:242-261 | MS.gene063690:CDS | 40.0% | |
| AGTTGTTCTCCTACTTGATG+AGG | + | 57340:867-886 | MS.gene063690:intron | 40.0% | |
| CACAAAAAGTTGACCTCAGA+AGG | + | 57340:1016-1035 | MS.gene063690:CDS | 40.0% | |
| CATTAACAACACATTGGGCA+AGG | + | 57340:1623-1642 | MS.gene063690:CDS | 40.0% | |
| CCGAGACATTAACAACACAT+TGG | + | 57340:1617-1636 | MS.gene063690:CDS | 40.0% | |
| GCATGCAAAGACTTGTGTAA+TGG | + | 57340:1494-1513 | MS.gene063690:CDS | 40.0% | |
| GCTACCTGGTATATATGCTT+TGG | + | 57340:681-700 | MS.gene063690:intron | 40.0% | |
| GGTGATAATTGTCTAGAGAC+CGG | + | 57340:2059-2078 | MS.gene063690:CDS | 40.0% | |
| GTGAACTCAATATCCCCAAT+TGG | - | 57340:229-248 | None:intergenic | 40.0% | |
| GTTTGGCGTTTAGAGAAGAT+TGG | + | 57340:977-996 | MS.gene063690:CDS | 40.0% | |
| TAAGGTAGGCCATTCATGAA+TGG | - | 57340:2158-2177 | None:intergenic | 40.0% | |
| TAGTGTCATCCTCATCAAGT+AGG | - | 57340:879-898 | None:intergenic | 40.0% | |
| TCACTATACAGGTGAAACCA+TGG | + | 57340:1974-1993 | MS.gene063690:intron | 40.0% | |
| TGGTATGTCCGAGAAAATGT+GGG | + | 57340:1458-1477 | MS.gene063690:CDS | 40.0% | |
| TTACCTTTCTGAGCCTAAGA+GGG | - | 57340:1084-1103 | None:intergenic | 40.0% | |
| TTCCCAGATTCACAATCAGA+CGG | + | 57340:2182-2201 | MS.gene063690:CDS | 40.0% | |
| TTGGTATGTCCGAGAAAATG+TGG | + | 57340:1457-1476 | MS.gene063690:CDS | 40.0% | |
| ! | GTGTTGTTAATGTCTCGGAT+GGG | - | 57340:1615-1634 | None:intergenic | 40.0% |
| ! | GTTGTTTGCTACATAGTTGC+CGG | - | 57340:2081-2100 | None:intergenic | 40.0% |
| ! | TGAGAGAAGCATATGCTAGA+TGG | + | 57340:1752-1771 | MS.gene063690:CDS | 40.0% |
| ! | TGTCACATCCCACATTTTCT+CGG | - | 57340:1469-1488 | None:intergenic | 40.0% |
| ! | TGTGTTGTTAATGTCTCGGA+TGG | - | 57340:1616-1635 | None:intergenic | 40.0% |
| ! | TTGCTGTTAGAATTGCTCCT+GGG | + | 57340:287-306 | MS.gene063690:CDS | 40.0% |
| !! | TTTGTTGTTAAGGATCACCG+TGG | + | 57340:352-371 | MS.gene063690:CDS | 40.0% |
| !!! | GACCGAAGCTTTTGTTGTTA+AGG | + | 57340:342-361 | MS.gene063690:CDS | 40.0% |
| AATCTGGGAAGCTGTAAGGT+AGG | - | 57340:2172-2191 | None:intergenic | 45.0% | |
| AGGGATGGAATTGCACCAAT+TGG | + | 57340:211-230 | MS.gene063690:CDS | 45.0% | |
| CACCGTCTGATTGTGAATCT+GGG | - | 57340:2187-2206 | None:intergenic | 45.0% | |
| GCCACATCACCATTCATGAA+TGG | + | 57340:2146-2165 | MS.gene063690:CDS | 45.0% | |
| GGATGGAATTGCACCAATTG+GGG | + | 57340:213-232 | MS.gene063690:CDS | 45.0% | |
| GGGATGGAATTGCACCAATT+GGG | + | 57340:212-231 | MS.gene063690:CDS | 45.0% | |
| GTTAATGTCTCGGATGGGAA+TGG | - | 57340:1610-1629 | None:intergenic | 45.0% | |
| GTTACCTTTCTGAGCCTAAG+AGG | - | 57340:1085-1104 | None:intergenic | 45.0% | |
| TGTCTGTAGTTGACCCTCTT+AGG | + | 57340:1068-1087 | MS.gene063690:CDS | 45.0% | |
| TGTGAATCTGGGAAGCTGTA+AGG | - | 57340:2176-2195 | None:intergenic | 45.0% | |
| ! | CAAAGTGTCCGAATTCGTGA+AGG | + | 57340:316-335 | MS.gene063690:CDS | 45.0% |
| ! | CACAAATAGGTTGCATCGAC+TGG | + | 57340:2111-2130 | MS.gene063690:CDS | 45.0% |
| ! | GCCATTCATGAATGGTGATG+TGG | - | 57340:2150-2169 | None:intergenic | 45.0% |
| ! | GTTGCTGTTAGAATTGCTCC+TGG | + | 57340:286-305 | MS.gene063690:CDS | 45.0% |
| ! | TGCTAGATGGAATGAGTTGG+AGG | + | 57340:1765-1784 | MS.gene063690:CDS | 45.0% |
| ! | TGCTAGCAGATTTTAGGCGT+TGG | + | 57340:1438-1457 | MS.gene063690:intron | 45.0% |
| !!! | TTTTTGTTTAATTTATTTTA+CGG | + | 57340:782-801 | MS.gene063690:intron | 5.0% |
| CCACCGTCTGATTGTGAATC+TGG | - | 57340:2188-2207 | None:intergenic | 50.0% | |
| CCAGATTCACAATCAGACGG+TGG | + | 57340:2185-2204 | MS.gene063690:CDS | 50.0% | |
| CGGTCATGCCTTCACGAATT+CGG | - | 57340:327-346 | None:intergenic | 50.0% | |
| GGATGGTAAATTGAGGACCG+CGG | - | 57340:1539-1558 | None:intergenic | 50.0% | |
| TGACCCTCTTAGGCTCAGAA+AGG | + | 57340:1078-1097 | MS.gene063690:CDS | 50.0% | |
| TGTCTCGGATGGGAATGGTT+TGG | - | 57340:1605-1624 | None:intergenic | 50.0% | |
| ! | CGGACACTTTGATTAGTCCC+AGG | - | 57340:307-326 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57340 | gene | 208 | 2226 | 208 | ID=MS.gene063690 |
| 57340 | mRNA | 208 | 2226 | 208 | ID=MS.gene063690.t1;Parent=MS.gene063690 |
| 57340 | exon | 208 | 379 | 208 | ID=MS.gene063690.t1.exon1;Parent=MS.gene063690.t1 |
| 57340 | CDS | 208 | 379 | 208 | ID=cds.MS.gene063690.t1;Parent=MS.gene063690.t1 |
| 57340 | exon | 939 | 1099 | 939 | ID=MS.gene063690.t1.exon2;Parent=MS.gene063690.t1 |
| 57340 | CDS | 939 | 1099 | 939 | ID=cds.MS.gene063690.t1;Parent=MS.gene063690.t1 |
| 57340 | exon | 1447 | 1644 | 1447 | ID=MS.gene063690.t1.exon3;Parent=MS.gene063690.t1 |
| 57340 | CDS | 1447 | 1644 | 1447 | ID=cds.MS.gene063690.t1;Parent=MS.gene063690.t1 |
| 57340 | exon | 1733 | 1832 | 1733 | ID=MS.gene063690.t1.exon4;Parent=MS.gene063690.t1 |
| 57340 | CDS | 1733 | 1832 | 1733 | ID=cds.MS.gene063690.t1;Parent=MS.gene063690.t1 |
| 57340 | exon | 1985 | 2226 | 1985 | ID=MS.gene063690.t1.exon5;Parent=MS.gene063690.t1 |
| 57340 | CDS | 1985 | 2226 | 1985 | ID=cds.MS.gene063690.t1;Parent=MS.gene063690.t1 |
| Gene Sequence |
| Protein sequence |
