AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064509

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064509.t1 MTR_5g078030 98.347 242 4 0 1 242 37 278 9.24e-176 484
MS.gene064509.t1 MTR_3g065370 68.033 244 70 3 2 242 38 276 2.07e-112 324
MS.gene064509.t1 MTR_8g104990 51.220 246 113 2 2 240 35 280 1.54e-86 258
MS.gene064509.t1 MTR_1g080350 30.952 252 150 11 8 242 48 292 3.52e-23 95.5
MS.gene064509.t1 MTR_1g044580 31.020 245 160 5 4 241 48 290 6.12e-23 97.4
MS.gene064509.t1 MTR_7g098410 27.386 241 158 5 11 240 47 281 1.46e-22 93.6
MS.gene064509.t1 MTR_1g073320 28.627 255 161 6 4 240 46 297 6.79e-22 92.8
MS.gene064509.t1 MTR_2g088980 29.500 200 130 2 2 190 39 238 7.37e-22 90.9
MS.gene064509.t1 MTR_2g088930 28.358 201 133 4 2 191 39 239 6.59e-20 85.9
MS.gene064509.t1 MTR_5g005480 29.528 254 148 7 2 233 41 285 1.04e-18 85.1
MS.gene064509.t1 MTR_6g055960 26.389 216 119 3 3 190 34 237 8.68e-18 82.4
MS.gene064509.t1 MTR_1g105650 28.155 206 129 4 3 191 45 248 1.05e-16 79.3
MS.gene064509.t1 MTR_3g011890 25.389 193 133 2 8 190 47 238 1.24e-16 77.0
MS.gene064509.t1 MTR_1g105655 26.070 257 164 8 2 235 47 300 2.84e-16 78.2
MS.gene064509.t1 MTR_3g011910 25.490 204 133 4 4 192 55 254 1.48e-15 75.9
MS.gene064509.t1 MTR_1g104890 23.664 262 178 6 2 242 51 311 1.48e-15 75.1
MS.gene064509.t1 MTR_3g011910 25.490 204 133 4 4 192 29 228 1.80e-15 75.5
MS.gene064509.t1 MTR_8g041690 24.365 197 137 2 6 190 43 239 1.83e-15 75.5
MS.gene064509.t1 MTR_7g052020 26.768 198 122 5 10 191 42 232 2.42e-15 73.6
MS.gene064509.t1 MTR_1g104890 23.664 262 178 6 2 242 51 311 3.90e-15 74.3
MS.gene064509.t1 MTR_6g463400 26.733 202 133 3 4 190 42 243 6.04e-15 73.9
MS.gene064509.t1 MTR_1g104890 23.664 262 178 6 2 242 51 311 6.40e-15 73.9
MS.gene064509.t1 MTR_1g104890 23.664 262 178 6 2 242 51 311 7.80e-15 73.6
MS.gene064509.t1 MTR_5g068260 26.341 205 132 4 2 191 39 239 5.24e-14 71.2
MS.gene064509.t1 MTR_5g005450 28.042 189 107 3 2 169 41 221 1.01e-13 70.5
MS.gene064509.t1 MTR_1g021610 26.316 209 133 4 2 193 44 248 1.16e-13 70.5
MS.gene064509.t1 MTR_1g021635 27.619 210 131 7 1 192 41 247 1.16e-13 70.5
MS.gene064509.t1 MTR_6g463710 28.230 209 124 5 3 190 42 245 1.35e-13 70.1
MS.gene064509.t1 MTR_6g057770 28.230 209 124 5 3 190 42 245 1.35e-13 70.1
MS.gene064509.t1 MTR_6g053000 26.404 178 117 4 3 168 39 214 3.21e-13 68.9
MS.gene064509.t1 MTR_5g068250 26.214 206 133 4 2 192 44 245 4.17e-13 68.6
MS.gene064509.t1 MTR_7g033135 32.692 156 97 4 42 192 80 232 5.05e-13 68.6
MS.gene064509.t1 MTR_8g041710 22.925 253 178 5 5 241 43 294 1.07e-12 67.4
MS.gene064509.t1 MTR_8g041650 25.749 167 110 3 8 162 50 214 2.24e-12 66.6
MS.gene064509.t1 MTR_1g105615 25.837 209 133 5 2 191 42 247 2.85e-12 66.2
MS.gene064509.t1 MTR_8g041880 26.344 186 124 2 3 175 41 226 2.98e-12 66.2
MS.gene064509.t1 MTR_5g068190 26.667 210 132 5 2 192 37 243 7.17e-12 65.1
MS.gene064509.t1 MTR_0007s0020 26.316 209 131 5 3 190 39 245 1.52e-11 64.3
MS.gene064509.t1 MTR_8g028065 26.136 176 124 1 21 190 61 236 3.45e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064509.t1 AT5G37660 62.810 242 89 1 2 242 47 288 7.67e-101 295
MS.gene064509.t1 AT5G37660 62.656 241 89 1 2 241 47 287 5.62e-100 293
MS.gene064509.t1 AT2G01660 55.870 247 100 4 2 239 37 283 4.99e-91 270
MS.gene064509.t1 AT2G01660 55.870 247 100 4 2 239 37 283 4.99e-91 270
MS.gene064509.t1 AT2G01660 59.615 208 80 3 2 205 37 244 4.67e-83 249
MS.gene064509.t1 AT1G70690 45.418 251 124 4 2 239 41 291 2.44e-66 207
MS.gene064509.t1 AT3G60720 39.095 243 142 3 2 239 39 280 8.36e-53 172
MS.gene064509.t1 AT3G60720 39.669 242 136 4 2 238 39 275 9.99e-53 172
MS.gene064509.t1 AT3G60720 39.130 207 121 2 2 203 39 245 6.15e-46 154
MS.gene064509.t1 AT1G04520 27.481 262 159 4 4 240 48 303 1.19e-20 89.0
MS.gene064509.t1 AT2G33330 26.877 253 168 3 4 240 49 300 1.46e-20 88.6
MS.gene064509.t1 AT5G48540 27.000 200 131 3 5 190 37 235 1.55e-18 82.4
MS.gene064509.t1 AT5G43980 28.077 260 165 6 4 242 41 299 4.07e-18 82.0
MS.gene064509.t1 AT1G63550 26.570 207 135 4 2 193 42 246 2.29e-17 80.1
MS.gene064509.t1 AT1G63580 25.210 238 153 7 8 222 37 272 1.66e-15 74.3
MS.gene064509.t1 AT3G22060 30.542 203 122 6 2 190 46 243 1.20e-14 71.6
MS.gene064509.t1 AT5G41290 24.883 213 127 6 3 192 41 243 2.26e-14 71.2
MS.gene064509.t1 AT5G40380 29.353 201 118 7 42 231 84 271 9.81e-14 70.5
MS.gene064509.t1 AT5G40380 29.353 201 118 7 42 231 84 271 1.17e-13 70.5
MS.gene064509.t1 AT5G40380 29.353 201 118 7 42 231 84 271 1.20e-13 70.5
MS.gene064509.t1 AT5G41300 23.645 203 138 3 3 190 45 245 2.05e-13 68.6
MS.gene064509.t1 AT4G23310 28.440 218 138 5 1 203 38 252 3.54e-13 68.9
MS.gene064509.t1 AT4G23310 28.440 218 138 5 1 203 38 252 4.67e-13 68.6
MS.gene064509.t1 AT1G63570 26.794 209 130 5 2 192 38 241 6.98e-13 67.0
MS.gene064509.t1 AT1G63570 26.794 209 130 5 2 192 38 241 6.98e-13 67.0
MS.gene064509.t1 AT1G61750 20.755 265 174 5 2 231 40 303 7.20e-13 67.4
MS.gene064509.t1 AT1G63570 26.794 209 130 5 2 192 91 294 1.22e-12 66.6
MS.gene064509.t1 AT3G22010 25.581 215 126 9 2 191 51 256 3.66e-12 64.7
MS.gene064509.t1 AT3G21990 25.962 208 133 7 2 191 45 249 1.34e-11 63.2
MS.gene064509.t1 AT4G23280 26.432 227 137 8 2 204 39 259 1.42e-11 64.3
MS.gene064509.t1 AT4G23180 29.843 191 106 7 2 172 50 232 1.44e-11 64.3
MS.gene064509.t1 AT4G23280 26.432 227 137 8 2 204 39 259 1.49e-11 64.3
MS.gene064509.t1 AT4G23180 29.843 191 106 7 2 172 50 232 1.51e-11 63.9
MS.gene064509.t1 AT4G23280 26.432 227 137 8 2 204 39 259 1.80e-11 63.9
MS.gene064509.t1 AT4G23180 29.843 191 106 7 2 172 50 232 2.20e-11 63.5
MS.gene064509.t1 AT4G20640 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene064509.t1 AT4G20580 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene064509.t1 AT4G20610 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene064509.t1 AT4G20630 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene064509.t1 AT4G20620 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene064509.t1 AT4G20590 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene064509.t1 AT4G20600 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene064509.t1 AT4G20570 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene064509.t1 AT4G20560 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene064509.t1 AT4G20645 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene064509.t1 AT4G20550 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene064509.t1 AT4G20530 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene064509.t1 AT4G20540 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene064509.t1 AT4G20670 24.155 207 138 5 2 191 45 249 8.83e-11 60.8

Find 78 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTGAGCCCATGGCTTC+TGG 0.211124 5.3:-63131959 None:intergenic
TGTACTGGGGATCTTAGTTT+TGG 0.219626 5.3:+63132061 MS.gene064509:CDS
CCCGAACCACCAACCCTAAA+AGG 0.258817 5.3:-63132016 None:intergenic
TGGATGGCTTGCAGGAAAAT+TGG 0.277770 5.3:+63132973 MS.gene064509:intron
CTTTCTTGAGCAAGATTTGT+AGG 0.299010 5.3:+63133071 MS.gene064509:CDS
CTTGGTGGGCTTGTGGGCTT+AGG 0.300238 5.3:+63131989 MS.gene064509:CDS
CGTGGTGTTGCCCAGTGTAC+TGG 0.317322 5.3:+63132046 MS.gene064509:CDS
GCTGTGCTTGGTGGGCTTGT+GGG 0.350767 5.3:+63131983 MS.gene064509:CDS
GATTGTGGTACTGCAGATTA+TGG 0.358387 5.3:+63132130 MS.gene064509:CDS
GCACGCGCTGTCACACGTGC+TGG 0.363096 5.3:+63131800 MS.gene064509:CDS
AAGAGATTTGCATTAATCAT+TGG 0.369856 5.3:+63133010 MS.gene064509:CDS
GATTATGGAGATTTGTTCTT+GGG 0.375248 5.3:+63132145 MS.gene064509:CDS
AGCAATGAGAATAACAAGTA+TGG 0.392517 5.3:-63133050 None:intergenic
TTTGACTCGTAGGGTGAGTT+TGG 0.395292 5.3:-63131626 None:intergenic
TGCTGTGCTTGGTGGGCTTG+TGG 0.401298 5.3:+63131982 MS.gene064509:CDS
GGTGGGCTTGTGGGCTTAGG+TGG 0.405467 5.3:+63131992 MS.gene064509:CDS
TTAATCATTGGTTTATTAGC+AGG 0.406034 5.3:+63133022 MS.gene064509:CDS
TCAAGAGATGCTGTGCTTGG+TGG 0.408146 5.3:+63131974 MS.gene064509:CDS
GGTCGCCACGGCATTGATAT+AGG 0.413623 5.3:-63131748 None:intergenic
GGTCCAGCTCGACGGTTGTT+TGG 0.415998 5.3:+63131853 MS.gene064509:CDS
GGCCATAGATTATGTCTTGT+TGG 0.417754 5.3:-63131727 None:intergenic
AGATTATGGAGATTTGTTCT+TGG 0.419653 5.3:+63132144 MS.gene064509:CDS
CAAGAGATGCTGTGCTTGGT+GGG 0.419763 5.3:+63131975 MS.gene064509:CDS
GTGGGCTTGTGGGCTTAGGT+GGG 0.426219 5.3:+63131993 MS.gene064509:CDS
GCCATAGATTATGTCTTGTT+GGG 0.429320 5.3:-63131726 None:intergenic
TAGGGTTGGTGGTTCGGGTA+TGG 0.430373 5.3:+63132021 MS.gene064509:CDS
TGGGGCCCATGATACGTCTA+AGG 0.432532 5.3:+63132195 MS.gene064509:CDS
CACGCGCTGTCACACGTGCT+GGG 0.445092 5.3:+63131801 MS.gene064509:CDS
ACCCAACAAGACATAATCTA+TGG 0.445749 5.3:+63131725 MS.gene064509:CDS
GTGGTGTTGCCCAGTGTACT+GGG 0.465182 5.3:+63132047 MS.gene064509:CDS
TACAACAACTTCACCGTCCT+AGG 0.469752 5.3:+63131698 MS.gene064509:CDS
GCAGGAAAATTGGGTATAGT+AGG 0.470565 5.3:+63132983 MS.gene064509:intron
CATGGGCCTTAGACGTATCA+TGG 0.471929 5.3:-63132201 None:intergenic
GGCTCAAGAGATGCTGTGCT+TGG 0.472019 5.3:+63131971 MS.gene064509:CDS
GTGAGGTGAGGAGTGAATTG+AGG 0.473079 5.3:-63131649 None:intergenic
GGATGGCTTGCAGGAAAATT+GGG 0.474228 5.3:+63132974 MS.gene064509:intron
TCGCGACTCGTGCGGAGGCA+CGG 0.481840 5.3:+63131832 MS.gene064509:CDS
TGTCTTGTTGGGTGGACCCT+AGG 0.483605 5.3:-63131715 None:intergenic
TGAATTGAGGTTTGACTCGT+AGG 0.499158 5.3:-63131636 None:intergenic
CCGTGGCGACCTCGCTATGC+CGG 0.500432 5.3:+63131760 MS.gene064509:CDS
CACAGCATCTCTTGAGCCCA+TGG 0.506789 5.3:-63131966 None:intergenic
AACTAAGATCCCCAGTACAC+TGG 0.510980 5.3:-63132057 None:intergenic
TAGGACGGTGAAGTTGTTGT+AGG 0.515242 5.3:-63131696 None:intergenic
TCACCAAACAACCGTCGAGC+TGG 0.516521 5.3:-63131856 None:intergenic
ATAGATTATGTCTTGTTGGG+TGG 0.527972 5.3:-63131723 None:intergenic
GGGCTATAATCCAGAAGCCA+TGG 0.539733 5.3:+63131949 MS.gene064509:CDS
ACTAAGATCCCCAGTACACT+GGG 0.557663 5.3:-63132056 None:intergenic
GCGACACAGGAGGCACAGTC+CGG 0.560545 5.3:-63131779 None:intergenic
TGTGACAGCGCGTGCGACAC+AGG 0.562753 5.3:-63131792 None:intergenic
GGCTATAATCCAGAAGCCAT+GGG 0.566857 5.3:+63131950 MS.gene064509:CDS
AGACGTATCATGGGCCCCAC+CGG 0.577254 5.3:-63132191 None:intergenic
ATGCAAGATATTCTACCGGT+GGG 0.580075 5.3:+63132176 MS.gene064509:CDS
GTGTTGTAGAAGCGATCGGA+CGG 0.581568 5.3:+63132098 MS.gene064509:CDS
GAATTGAGGTTTGACTCGTA+GGG 0.593749 5.3:-63131635 None:intergenic
ACAACAACTTCACCGTCCTA+GGG 0.596633 5.3:+63131699 MS.gene064509:CDS
GGAGGCACGGTCCAGCTCGA+CGG 0.596985 5.3:+63131845 MS.gene064509:CDS
GGTGGTTCGGGTATGGTACG+TGG 0.597946 5.3:+63132028 MS.gene064509:CDS
AGCAAGATTTGTAGGAGACA+AGG 0.603122 5.3:+63133079 MS.gene064509:CDS
ACGCGCTGTCACACGTGCTG+GGG 0.608382 5.3:+63131802 MS.gene064509:CDS
CCGGCATAGCGAGGTCGCCA+CGG 0.614398 5.3:-63131760 None:intergenic
ATGTGTCGCGACTCGTGCGG+AGG 0.615055 5.3:+63131827 MS.gene064509:CDS
GGCTTCTGGATTATAGCCCA+CGG 0.615132 5.3:-63131945 None:intergenic
GGACGGTTGAAAAGTGATTG+TGG 0.615271 5.3:+63132115 MS.gene064509:CDS
TGTTATGCAAGATATTCTAC+CGG 0.616220 5.3:+63132172 MS.gene064509:CDS
AGGCACAGTCCGGCATAGCG+AGG 0.619370 5.3:-63131769 None:intergenic
GATTGTGTTGTAGAAGCGAT+CGG 0.619387 5.3:+63132094 MS.gene064509:CDS
TATGCAAGATATTCTACCGG+TGG 0.627330 5.3:+63132175 MS.gene064509:CDS
CATGATACGTCTAAGGCCCA+TGG 0.629745 5.3:+63132202 MS.gene064509:CDS
ATGGGCCTTAGACGTATCAT+GGG 0.630265 5.3:-63132200 None:intergenic
TCTGGATTATAGCCCACGGA+AGG 0.644583 5.3:-63131941 None:intergenic
TGCAAGATATTCTACCGGTG+GGG 0.653008 5.3:+63132177 MS.gene064509:CDS
TTGTTGGGTGGACCCTAGGA+CGG 0.669929 5.3:-63131711 None:intergenic
CTGAGTTGACTAGTGAGGTG+AGG 0.674733 5.3:-63131661 None:intergenic
GACATGTGTCGCGACTCGTG+CGG 0.678683 5.3:+63131824 MS.gene064509:CDS
TATGGCCTATATCAATGCCG+TGG 0.684424 5.3:+63131743 MS.gene064509:CDS
GACAGCGCGTGCGACACAGG+AGG 0.694407 5.3:-63131789 None:intergenic
TGGTGTTGCCCAGTGTACTG+GGG 0.727512 5.3:+63132048 MS.gene064509:CDS
TGTTGCTGAGTTGACTAGTG+AGG 0.747592 5.3:-63131666 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAAACATATAACCAT+TGG + chr5.3:63133181-63133200 MS.gene064509:intron 15.0%
!! AATGTTTACAAATTACAAAT+TGG - chr5.3:63132506-63132525 None:intergenic 15.0%
!! ATGTTGAAATAATAAAACAT+TGG - chr5.3:63132583-63132602 None:intergenic 15.0%
!! ATTTACTTGTGAAAATATTA+GGG + chr5.3:63132661-63132680 MS.gene064509:intron 15.0%
!! TATTTACTTGTGAAAATATT+AGG + chr5.3:63132660-63132679 MS.gene064509:intron 15.0%
!! TGAATTGATTATATTAGTTA+AGG - chr5.3:63133152-63133171 None:intergenic 15.0%
!! TGTTGAAATAATAAAACATT+GGG - chr5.3:63132582-63132601 None:intergenic 15.0%
!! AGAGATTTGTAAGAAAAAAA+AGG + chr5.3:63132610-63132629 MS.gene064509:intron 20.0%
!!! ATGTTTTCTTTTACTTTGTT+AGG + chr5.3:63132441-63132460 MS.gene064509:intron 20.0%
!!! CTACTTCTTTTTTAATCAAA+AGG + chr5.3:63132268-63132287 MS.gene064509:intron 20.0%
!!! TGTTTTCTTTTACTTTGTTA+GGG + chr5.3:63132442-63132461 MS.gene064509:intron 20.0%
!!! TTTTTTCTTACAAATCTCTT+TGG - chr5.3:63132609-63132628 None:intergenic 20.0%
! AAGAGATTTGCATTAATCAT+TGG + chr5.3:63133010-63133029 MS.gene064509:CDS 25.0%
! ACATGTGATCAAAATTCAAA+GGG - chr5.3:63132927-63132946 None:intergenic 25.0%
! AGGAATTAATTGAAGAGTAA+TGG + chr5.3:63132288-63132307 MS.gene064509:intron 25.0%
! TTACAAATTACAAATTGGCA+TGG - chr5.3:63132501-63132520 None:intergenic 25.0%
!! GTAATTTGTAAACATTGACT+TGG + chr5.3:63132510-63132529 MS.gene064509:intron 25.0%
!!! AATGTTTTGTTGAAAAAGTG+TGG + chr5.3:63131917-63131936 MS.gene064509:CDS 25.0%
!!! AATTACCTGACTTATTTTGT+TGG + chr5.3:63132953-63132972 MS.gene064509:intron 25.0%
!!! ATGACTTTTTTTCTTTTGAC+CGG - chr5.3:63132423-63132442 None:intergenic 25.0%
!!! ATGTTTTGTTGAAAAAGTGT+GGG + chr5.3:63131918-63131937 MS.gene064509:CDS 25.0%
!!! TTAATCATTGGTTTATTAGC+AGG + chr5.3:63133022-63133041 MS.gene064509:CDS 25.0%
!!! TTTCAAGTATGTTTTTCTTC+CGG + chr5.3:63132401-63132420 MS.gene064509:intron 25.0%
AGCAATGAGAATAACAAGTA+TGG - chr5.3:63133053-63133072 None:intergenic 30.0%
CAAGATATTGCTTAATCCAA+TGG - chr5.3:63133200-63133219 None:intergenic 30.0%
CACATGTGATCAAAATTCAA+AGG - chr5.3:63132928-63132947 None:intergenic 30.0%
TGTTATGCAAGATATTCTAC+CGG + chr5.3:63132172-63132191 MS.gene064509:CDS 30.0%
! AATTGAAGAGTAATGGAGAT+TGG + chr5.3:63132295-63132314 MS.gene064509:intron 30.0%
! AGATTATGGAGATTTGTTCT+TGG + chr5.3:63132144-63132163 MS.gene064509:CDS 30.0%
! ATTGAAGAGTAATGGAGATT+GGG + chr5.3:63132296-63132315 MS.gene064509:intron 30.0%
! GATTATGGAGATTTGTTCTT+GGG + chr5.3:63132145-63132164 MS.gene064509:CDS 30.0%
!! CTTTGAATTTTGATCACATG+TGG + chr5.3:63132926-63132945 MS.gene064509:intron 30.0%
!!! TATGACAATGCTACTTTTCT+AGG + chr5.3:63131881-63131900 MS.gene064509:CDS 30.0%
AAAAGAAGTAGAAGCAGCAA+AGG - chr5.3:63132259-63132278 None:intergenic 35.0%
ACCCAACAAGACATAATCTA+TGG + chr5.3:63131725-63131744 MS.gene064509:CDS 35.0%
CTTTCTTGAGCAAGATTTGT+AGG + chr5.3:63133071-63133090 MS.gene064509:CDS 35.0%
GAGACAAGGTAAGTAGTATT+AGG + chr5.3:63133093-63133112 MS.gene064509:intron 35.0%
! ATAGATTATGTCTTGTTGGG+TGG - chr5.3:63131726-63131745 None:intergenic 35.0%
! GCCATAGATTATGTCTTGTT+GGG - chr5.3:63131729-63131748 None:intergenic 35.0%
!! AAACATTGACTTGGAGAATG+TGG + chr5.3:63132519-63132538 MS.gene064509:intron 35.0%
!!! ACCTGACTTATTTTGTTGGA+TGG + chr5.3:63132957-63132976 MS.gene064509:intron 35.0%
ACATGAGAAAGAACCCAAGA+TGG - chr5.3:63132234-63132253 None:intergenic 40.0%
AGCAAGATTTGTAGGAGACA+AGG + chr5.3:63133079-63133098 MS.gene064509:CDS 40.0%
ATGCAAGATATTCTACCGGT+GGG + chr5.3:63132176-63132195 MS.gene064509:CDS 40.0%
CATGAGAAAGAACCCAAGAT+GGG - chr5.3:63132233-63132252 None:intergenic 40.0%
GATTGTGGTACTGCAGATTA+TGG + chr5.3:63132130-63132149 MS.gene064509:CDS 40.0%
GATTGTGTTGTAGAAGCGAT+CGG + chr5.3:63132094-63132113 MS.gene064509:CDS 40.0%
GCAGGAAAATTGGGTATAGT+AGG + chr5.3:63132983-63133002 MS.gene064509:intron 40.0%
GCCATCCAACAAAATAAGTC+AGG - chr5.3:63132961-63132980 None:intergenic 40.0%
GGCCATAGATTATGTCTTGT+TGG - chr5.3:63131730-63131749 None:intergenic 40.0%
TATGCAAGATATTCTACCGG+TGG + chr5.3:63132175-63132194 MS.gene064509:CDS 40.0%
! GAATTGAGGTTTGACTCGTA+GGG - chr5.3:63131638-63131657 None:intergenic 40.0%
! TGAATTGAGGTTTGACTCGT+AGG - chr5.3:63131639-63131658 None:intergenic 40.0%
! TGTACTGGGGATCTTAGTTT+TGG + chr5.3:63132061-63132080 MS.gene064509:CDS 40.0%
!! GTACTGGGGATCTTAGTTTT+GGG + chr5.3:63132062-63132081 MS.gene064509:CDS 40.0%
!! TATTTTGTTGGATGGCTTGC+AGG + chr5.3:63132965-63132984 MS.gene064509:intron 40.0%
!!! ACAGCGTTTGTCGTTTTTTC+AGG + chr5.3:63132466-63132485 MS.gene064509:intron 40.0%
!!! CAATGCTACTTTTCTAGGAG+TGG + chr5.3:63131886-63131905 MS.gene064509:CDS 40.0%
!!! CTTGTATCTTTTTTCCGTGC+AGG + chr5.3:63133237-63133256 MS.gene064509:intron 40.0%
!!! TACTGGGGATCTTAGTTTTG+GGG + chr5.3:63132063-63132082 MS.gene064509:CDS 40.0%
AACTAAGATCCCCAGTACAC+TGG - chr5.3:63132060-63132079 None:intergenic 45.0%
ACAACAACTTCACCGTCCTA+GGG + chr5.3:63131699-63131718 MS.gene064509:CDS 45.0%
ACTAAGATCCCCAGTACACT+GGG - chr5.3:63132059-63132078 None:intergenic 45.0%
ATGGGCCTTAGACGTATCAT+GGG - chr5.3:63132203-63132222 None:intergenic 45.0%
GGACGGTTGAAAAGTGATTG+TGG + chr5.3:63132115-63132134 MS.gene064509:CDS 45.0%
GGCTATAATCCAGAAGCCAT+GGG + chr5.3:63131950-63131969 MS.gene064509:CDS 45.0%
TACAACAACTTCACCGTCCT+AGG + chr5.3:63131698-63131717 MS.gene064509:CDS 45.0%
TAGGACGGTGAAGTTGTTGT+AGG - chr5.3:63131699-63131718 None:intergenic 45.0%
TATGGCCTATATCAATGCCG+TGG + chr5.3:63131743-63131762 MS.gene064509:CDS 45.0%
TGCAAGATATTCTACCGGTG+GGG + chr5.3:63132177-63132196 MS.gene064509:CDS 45.0%
! GAAGAGTAATGGAGATTGGG+TGG + chr5.3:63132299-63132318 MS.gene064509:intron 45.0%
! GGATGGCTTGCAGGAAAATT+GGG + chr5.3:63132974-63132993 MS.gene064509:intron 45.0%
! TGGATGGCTTGCAGGAAAAT+TGG + chr5.3:63132973-63132992 MS.gene064509:intron 45.0%
! TGTTGCTGAGTTGACTAGTG+AGG - chr5.3:63131669-63131688 None:intergenic 45.0%
! TTTGACTCGTAGGGTGAGTT+TGG - chr5.3:63131629-63131648 None:intergenic 45.0%
!!! TGCTACTTTTCTAGGAGTGG+AGG + chr5.3:63131889-63131908 MS.gene064509:CDS 45.0%
CAAGAGATGCTGTGCTTGGT+GGG + chr5.3:63131975-63131994 MS.gene064509:CDS 50.0%
CATGGGCCTTAGACGTATCA+TGG - chr5.3:63132204-63132223 None:intergenic 50.0%
CCCATGGTAAAGCCCATCTT+GGG + chr5.3:63132218-63132237 MS.gene064509:intron 50.0%
GGCTTCTGGATTATAGCCCA+CGG - chr5.3:63131948-63131967 None:intergenic 50.0%
GGGCTATAATCCAGAAGCCA+TGG + chr5.3:63131949-63131968 MS.gene064509:CDS 50.0%
GTGAGGTGAGGAGTGAATTG+AGG - chr5.3:63131652-63131671 None:intergenic 50.0%
GTGTTGTAGAAGCGATCGGA+CGG + chr5.3:63132098-63132117 MS.gene064509:CDS 50.0%
TCAAGAGATGCTGTGCTTGG+TGG + chr5.3:63131974-63131993 MS.gene064509:CDS 50.0%
TCTGGATTATAGCCCACGGA+AGG - chr5.3:63131944-63131963 None:intergenic 50.0%
! AAAAAGTGTGGGCCTTCCGT+GGG + chr5.3:63131929-63131948 MS.gene064509:CDS 50.0%
! ACCCAAGATGGGCTTTACCA+TGG - chr5.3:63132222-63132241 None:intergenic 50.0%
! CATGATACGTCTAAGGCCCA+TGG + chr5.3:63132202-63132221 MS.gene064509:CDS 50.0%
! CCCAAGATGGGCTTTACCAT+GGG - chr5.3:63132221-63132240 None:intergenic 50.0%
! CTGAGTTGACTAGTGAGGTG+AGG - chr5.3:63131664-63131683 None:intergenic 50.0%
! GAGTAATGGAGATTGGGTGG+TGG + chr5.3:63132302-63132321 MS.gene064509:intron 50.0%
!!! CCTTTTAGGGTTGGTGGTTC+GGG + chr5.3:63132016-63132035 MS.gene064509:CDS 50.0%
!!! GCCTTTTAGGGTTGGTGGTT+CGG + chr5.3:63132015-63132034 MS.gene064509:CDS 50.0%
!!! TTAGGTGGGCCTTTTAGGGT+TGG + chr5.3:63132007-63132026 MS.gene064509:CDS 50.0%
CACAGCATCTCTTGAGCCCA+TGG - chr5.3:63131969-63131988 None:intergenic 55.0%
CCCGAACCACCAACCCTAAA+AGG - chr5.3:63132019-63132038 None:intergenic 55.0%
GCCCATGGTAAAGCCCATCT+TGG + chr5.3:63132217-63132236 MS.gene064509:intron 55.0%
GGCTCAAGAGATGCTGTGCT+TGG + chr5.3:63131971-63131990 MS.gene064509:CDS 55.0%
TCACCAAACAACCGTCGAGC+TGG - chr5.3:63131859-63131878 None:intergenic 55.0%
TCTCTTGAGCCCATGGCTTC+TGG - chr5.3:63131962-63131981 None:intergenic 55.0%
TGGGGCCCATGATACGTCTA+AGG + chr5.3:63132195-63132214 MS.gene064509:CDS 55.0%
TTGTTGGGTGGACCCTAGGA+CGG - chr5.3:63131714-63131733 None:intergenic 55.0%
! GAAAAAGTGTGGGCCTTCCG+TGG + chr5.3:63131928-63131947 MS.gene064509:CDS 55.0%
! GGGCTTAGGTGGGCCTTTTA+GGG + chr5.3:63132003-63132022 MS.gene064509:CDS 55.0%
! TGGGCTTAGGTGGGCCTTTT+AGG + chr5.3:63132002-63132021 MS.gene064509:CDS 55.0%
! TGTCTTGTTGGGTGGACCCT+AGG - chr5.3:63131718-63131737 None:intergenic 55.0%
!! GGTCGCCACGGCATTGATAT+AGG - chr5.3:63131751-63131770 None:intergenic 55.0%
!! GTGGTGTTGCCCAGTGTACT+GGG + chr5.3:63132047-63132066 MS.gene064509:CDS 55.0%
!! TAGGGTTGGTGGTTCGGGTA+TGG + chr5.3:63132021-63132040 MS.gene064509:CDS 55.0%
!! TGGTGTTGCCCAGTGTACTG+GGG + chr5.3:63132048-63132067 MS.gene064509:CDS 55.0%
AGACGTATCATGGGCCCCAC+CGG - chr5.3:63132194-63132213 None:intergenic 60.0%
GACATGTGTCGCGACTCGTG+CGG + chr5.3:63131824-63131843 MS.gene064509:CDS 60.0%
GGTCCAGCTCGACGGTTGTT+TGG + chr5.3:63131853-63131872 MS.gene064509:CDS 60.0%
GTGGGCTTGTGGGCTTAGGT+GGG + chr5.3:63131993-63132012 MS.gene064509:CDS 60.0%
! GGTGGTTCGGGTATGGTACG+TGG + chr5.3:63132028-63132047 MS.gene064509:CDS 60.0%
!! CGTGGTGTTGCCCAGTGTAC+TGG + chr5.3:63132046-63132065 MS.gene064509:CDS 60.0%
!! CTTGGTGGGCTTGTGGGCTT+AGG + chr5.3:63131989-63132008 MS.gene064509:CDS 60.0%
!! GCTGTGCTTGGTGGGCTTGT+GGG + chr5.3:63131983-63132002 MS.gene064509:CDS 60.0%
!! TGCTGTGCTTGGTGGGCTTG+TGG + chr5.3:63131982-63132001 MS.gene064509:CDS 60.0%
!!! GGTGGGCCTTTTAGGGTTGG+TGG + chr5.3:63132010-63132029 MS.gene064509:CDS 60.0%
ACGCGCTGTCACACGTGCTG+GGG + chr5.3:63131802-63131821 MS.gene064509:CDS 65.0%
AGGCACAGTCCGGCATAGCG+AGG - chr5.3:63131772-63131791 None:intergenic 65.0%
ATGTGTCGCGACTCGTGCGG+AGG + chr5.3:63131827-63131846 MS.gene064509:CDS 65.0%
CACGCGCTGTCACACGTGCT+GGG + chr5.3:63131801-63131820 MS.gene064509:CDS 65.0%
TGTGACAGCGCGTGCGACAC+AGG - chr5.3:63131795-63131814 None:intergenic 65.0%
! GCGACACAGGAGGCACAGTC+CGG - chr5.3:63131782-63131801 None:intergenic 65.0%
! GGTGGGCTTGTGGGCTTAGG+TGG + chr5.3:63131992-63132011 MS.gene064509:CDS 65.0%
CCGGCATAGCGAGGTCGCCA+CGG - chr5.3:63131763-63131782 None:intergenic 70.0%
CCGTGGCGACCTCGCTATGC+CGG + chr5.3:63131760-63131779 MS.gene064509:CDS 70.0%
GACAGCGCGTGCGACACAGG+AGG - chr5.3:63131792-63131811 None:intergenic 70.0%
GCACGCGCTGTCACACGTGC+TGG + chr5.3:63131800-63131819 MS.gene064509:CDS 70.0%
TCGCGACTCGTGCGGAGGCA+CGG + chr5.3:63131832-63131851 MS.gene064509:CDS 70.0%
! GGAGGCACGGTCCAGCTCGA+CGG + chr5.3:63131845-63131864 MS.gene064509:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 63131617 63133266 63131617 ID=MS.gene064509
chr5.3 mRNA 63131617 63133266 63131617 ID=MS.gene064509.t1;Parent=MS.gene064509
chr5.3 exon 63131617 63132223 63131617 ID=MS.gene064509.t1.exon1;Parent=MS.gene064509.t1
chr5.3 CDS 63131617 63132223 63131617 ID=cds.MS.gene064509.t1;Parent=MS.gene064509.t1
chr5.3 exon 63132987 63133100 63132987 ID=MS.gene064509.t1.exon2;Parent=MS.gene064509.t1
chr5.3 CDS 63132987 63133100 63132987 ID=cds.MS.gene064509.t1;Parent=MS.gene064509.t1
chr5.3 exon 63133259 63133266 63133259 ID=MS.gene064509.t1.exon3;Parent=MS.gene064509.t1
chr5.3 CDS 63133259 63133266 63133259 ID=cds.MS.gene064509.t1;Parent=MS.gene064509.t1
Gene Sequence

>MS.gene064509

ATGTACACACCAAACTCACCCTACGAGTCAAACCTCAATTCACTCCTCACCTCACTAGTCAACTCAGCAACATACTCATCCTACAACAACTTCACCGTCCTAGGGTCCACCCAACAAGACATAATCTATGGCCTATATCAATGCCGTGGCGACCTCGCTATGCCGGACTGTGCCTCCTGTGTCGCACGCGCTGTCACACGTGCTGGGGACATGTGTCGCGACTCGTGCGGAGGCACGGTCCAGCTCGACGGTTGTTTGGTGAAGTATGACAATGCTACTTTTCTAGGAGTGGAGGATAAGAATGTTTTGTTGAAAAAGTGTGGGCCTTCCGTGGGCTATAATCCAGAAGCCATGGGCTCAAGAGATGCTGTGCTTGGTGGGCTTGTGGGCTTAGGTGGGCCTTTTAGGGTTGGTGGTTCGGGTATGGTACGTGGTGTTGCCCAGTGTACTGGGGATCTTAGTTTTGGGGAGTGTCAAGATTGTGTTGTAGAAGCGATCGGACGGTTGAAAAGTGATTGTGGTACTGCAGATTATGGAGATTTGTTCTTGGGTAAATGTTATGCAAGATATTCTACCGGTGGGGCCCATGATACGTCTAAGGCCCATGGTAAAGCCCATCTTGGGTTCTTTCTCATGTTACCTTTGCTGCTTCTACTTCTTTTTTAATCAAAAGGAATTAATTGAAGAGTAATGGAGATTGGGTGGTGGTGTAACTTAATTTGAATTGTAAATTTGTTTTTGATCAACTTTTTTTTTTTTCATTTGCAAACTGTAATATGCAAAATTTCAAGTATGTTTTTCTTCCGGTCAAAAGAAAAAAAGTCATGTTTTCTTTTACTTTGTTAGGGCACAGCGTTTGTCGTTTTTTCAGGATGATTCTTCCATGCCAATTTGTAATTTGTAAACATTGACTTGGAGAATGTGGTAGATTTAACTAAAATAAGTAAAAATATATATATATACCCAATGTTTTATTATTTCAACATGTTCCAAAGAGATTTGTAAGAAAAAAAAGGAACACACTTATTTTTCTTCTAATAGTTTATTTACTTGTGAAAATATTAGGGTTTGTTCTTTTATTAAAATATAAAAACATATTATGTTTTGTTATGCATATATTTTATTTGTACATTTTTTACTTTAATTGATATTACTTTGTTTCAGTTTTATAACATTCATATTATGCATGTATATAAATTTTAGTTTCAATATTCTAGTTGTATGTTTTTAAATTCAATTTTTTTTTTTAAATTATGTTATCAAAATAAAATTTATAGTACAAAAATATAATCAAAGTTGATGTTATTCCCTTTGAATTTTGATCACATGTGGTATAAATTACCTGACTTATTTTGTTGGATGGCTTGCAGGAAAATTGGGTATAGTAGGTGAAAAGAGATTTGCATTAATCATTGGTTTATTAGCAGGAGCAGCCATACTTGTTATTCTCATTGCTTTCTTGAGCAAGATTTGTAGGAGACAAGGTAAGTAGTATTAGGCTATTTCTTTTCTTTTTTTGTCTCTTTGATTTTCCTTAACTAATATAATCAATTCAGATCATAAAAAATTAAAACATATAACCATTGGATTAAGCAATATCTTGACATGATTTTTTTTGTGCTTGTATCTTTTTTCCGTGCAGGTAAATAA
Protein sequence

>MS.gene064509.t1

MYTPNSPYESNLNSLLTSLVNSATYSSYNNFTVLGSTQQDIIYGLYQCRGDLAMPDCASCVARAVTRAGDMCRDSCGGTVQLDGCLVKYDNATFLGVEDKNVLLKKCGPSVGYNPEAMGSRDAVLGGLVGLGGPFRVGGSGMVRGVAQCTGDLSFGECQDCVVEAIGRLKSDCGTADYGDLFLGKCYARYSTGGAHDTSKAHGKLGIVGEKRFALIIGLLAGAAILVILIAFLSKICRRQGK