Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064562.t1 | KEH19492.1 | 83.6 | 159 | 7 | 3 | 79 | 218 | 42 | 200 | 2.60E-66 | 261.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064562.t1 | A0A072TQ21 | 83.6 | 159 | 7 | 3 | 79 | 218 | 42 | 200 | 1.9e-66 | 261.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049756 | MS.gene064562 | 0.811689 | 6.18E-51 | -1.69E-46 |
| MS.gene054657 | MS.gene064562 | 0.804938 | 1.70E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene064562 | 0.812902 | 3.36E-51 | -1.69E-46 |
| MS.gene055603 | MS.gene064562 | 0.8071 | 5.96E-50 | -1.69E-46 |
| MS.gene056787 | MS.gene064562 | 0.80521 | 1.49E-49 | -1.69E-46 |
| MS.gene05795 | MS.gene064562 | 0.807802 | 4.23E-50 | -1.69E-46 |
| MS.gene058238 | MS.gene064562 | 0.801454 | 8.94E-49 | -1.69E-46 |
| MS.gene059830 | MS.gene064562 | 0.812596 | 3.92E-51 | -1.69E-46 |
| MS.gene060297 | MS.gene064562 | 0.813776 | 2.16E-51 | -1.69E-46 |
| MS.gene060586 | MS.gene064562 | 0.814159 | 1.78E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064562.t1 | MTR_8g461300 | 85.938 | 192 | 8 | 3 | 46 | 218 | 9 | 200 | 1.30e-115 | 328 |
| MS.gene064562.t1 | MTR_4g055800 | 51.497 | 167 | 67 | 6 | 60 | 216 | 130 | 292 | 1.17e-42 | 145 |
| MS.gene064562.t1 | MTR_3g080680 | 34.466 | 206 | 103 | 10 | 33 | 216 | 3 | 198 | 8.50e-20 | 84.0 |
| MS.gene064562.t1 | MTR_3g082420 | 33.182 | 220 | 112 | 9 | 28 | 216 | 6 | 221 | 1.59e-13 | 67.4 |
| MS.gene064562.t1 | MTR_3g083050 | 32.335 | 167 | 84 | 8 | 68 | 216 | 51 | 206 | 6.66e-12 | 62.8 |
| MS.gene064562.t1 | MTR_3g083080 | 34.286 | 175 | 82 | 10 | 60 | 216 | 46 | 205 | 6.64e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTTAAAAGTAATGTAATA+TGG | 0.262097 | 3.1:+41202673 | None:intergenic |
| GATTTCAGCCGTTTCGATTT+CGG | 0.272727 | 3.1:+41203392 | None:intergenic |
| TGGTTCTTTGTATACGAATA+AGG | 0.284069 | 3.1:-41203150 | MS.gene064562:CDS |
| GAATCTTCTGGATCCGGTTT+CGG | 0.312704 | 3.1:+41203251 | None:intergenic |
| TCACGACGCTTATACCAAAA+TGG | 0.333746 | 3.1:+41203173 | None:intergenic |
| TCACTATACTCAGAATCTTC+TGG | 0.339930 | 3.1:+41203239 | None:intergenic |
| GAAATCGAAACGGCTGAAAT+CGG | 0.340115 | 3.1:-41203390 | MS.gene064562:CDS |
| GTGTTTCAAAATGTCGAAAT+CGG | 0.387675 | 3.1:-41203423 | None:intergenic |
| GCTGAAATCGGAACCGAAAC+CGG | 0.414648 | 3.1:-41203264 | MS.gene064562:CDS |
| AAATCGAAACGGCTGAAATC+GGG | 0.418006 | 3.1:-41203389 | MS.gene064562:CDS |
| ATGAAACACTCTTTGACAAC+AGG | 0.423369 | 3.1:+41202574 | None:intergenic |
| CTACTTCTTTATATGCTCCT+AGG | 0.435411 | 3.1:-41202696 | MS.gene064562:CDS |
| ATCTGTGGAGGAGGAGGAGG+AGG | 0.436107 | 3.1:-41203294 | MS.gene064562:CDS |
| TACTCAGAATCTTCTGGATC+CGG | 0.439841 | 3.1:+41203245 | None:intergenic |
| TGCAGAGGGGTTTGAGAGTA+CGG | 0.440533 | 3.1:+41202899 | None:intergenic |
| GGAGGAGAAACGGCTGAAAT+CGG | 0.450703 | 3.1:-41203276 | MS.gene064562:CDS |
| TTCATCTGTGGAGGAGGAGG+AGG | 0.455235 | 3.1:-41203297 | MS.gene064562:CDS |
| CAAAATGTCGAAATCGGAAC+AGG | 0.457893 | 3.1:-41203417 | None:intergenic |
| CACTGTCCACCACGTTGTGC+TGG | 0.467693 | 3.1:-41202958 | MS.gene064562:CDS |
| TACGCTGCAATACATGAGTC+TGG | 0.467899 | 3.1:+41202608 | None:intergenic |
| AAAGTAATGTAATATGGCCT+AGG | 0.487724 | 3.1:+41202679 | None:intergenic |
| CAATCAAACAAATATTAAGC+AGG | 0.490074 | 3.1:-41202857 | MS.gene064562:intron |
| TAAAGCAAAAGCAGAAGCAA+AGG | 0.500294 | 3.1:-41202656 | MS.gene064562:CDS |
| TGAGGATGAGGAGGATAAGC+AGG | 0.508361 | 3.1:-41203336 | MS.gene064562:CDS |
| ACACGATGAAGATTCATATG+AGG | 0.514467 | 3.1:-41203354 | MS.gene064562:CDS |
| AGATTCATCTGTGGAGGAGG+AGG | 0.522683 | 3.1:-41203300 | MS.gene064562:CDS |
| GTCTGGAAAGTTGTTAGAGC+AGG | 0.523457 | 3.1:+41202625 | None:intergenic |
| GTTGTCAAGTGTACCCATCC+TGG | 0.532074 | 3.1:-41202721 | MS.gene064562:intron |
| AAAGCAAAAGCAGAAGCAAA+GGG | 0.538089 | 3.1:-41202655 | MS.gene064562:CDS |
| CTTTGTATACGAATAAGGCT+TGG | 0.542721 | 3.1:-41203145 | MS.gene064562:CDS |
| ACAAGATTCATCTGTGGAGG+AGG | 0.546974 | 3.1:-41203303 | MS.gene064562:CDS |
| TGAAGATTCATATGAGGATG+AGG | 0.548643 | 3.1:-41203348 | MS.gene064562:CDS |
| CACATCATCGCTTAAGTCGA+GGG | 0.551344 | 3.1:+41202924 | None:intergenic |
| AACAGGAACCGAAATCGAAA+CGG | 0.558710 | 3.1:-41203400 | MS.gene064562:CDS |
| CTTCAAAGCAGCATTGCAGA+GGG | 0.559702 | 3.1:+41202885 | None:intergenic |
| GTATACGAATAAGGCTTGGA+AGG | 0.564676 | 3.1:-41203141 | MS.gene064562:CDS |
| GCATATAAAGAAGTAGCACC+AGG | 0.567371 | 3.1:+41202703 | None:intergenic |
| TGTCCACCACGTTGTGCTGG+CGG | 0.572730 | 3.1:-41202955 | MS.gene064562:CDS |
| TAAGGCTTGGAAGGATCGTA+AGG | 0.585446 | 3.1:-41203132 | MS.gene064562:CDS |
| TAAAGAAGTAGCACCAGGAT+GGG | 0.590468 | 3.1:+41202708 | None:intergenic |
| GCACATCATCGCTTAAGTCG+AGG | 0.599300 | 3.1:+41202923 | None:intergenic |
| ATAAAGAAGTAGCACCAGGA+TGG | 0.610411 | 3.1:+41202707 | None:intergenic |
| CAGCACAACGTGGTGGACAG+TGG | 0.610706 | 3.1:+41202959 | None:intergenic |
| GAAACAAGATTCATCTGTGG+AGG | 0.613961 | 3.1:-41203306 | MS.gene064562:CDS |
| TCTTCAAAGCAGCATTGCAG+AGG | 0.630589 | 3.1:+41202884 | None:intergenic |
| AGATTCATATGAGGATGAGG+AGG | 0.633765 | 3.1:-41203345 | MS.gene064562:CDS |
| GAAACGGCTGAAATCGGGAT+CGG | 0.652067 | 3.1:-41203384 | MS.gene064562:CDS |
| AGAAGATTCTGAGTATAGTG+AGG | 0.652619 | 3.1:-41203237 | MS.gene064562:CDS |
| GCAAAAGCAGAAGCAAAGGG+AGG | 0.667232 | 3.1:-41202652 | MS.gene064562:CDS |
| TGAGTATAGTGAGGAAGACG+AGG | 0.671624 | 3.1:-41203228 | MS.gene064562:CDS |
| CATGAAACAAGATTCATCTG+TGG | 0.677002 | 3.1:-41203309 | MS.gene064562:CDS |
| ATACCGCCAGCACAACGTGG+TGG | 0.689012 | 3.1:+41202952 | None:intergenic |
| ACATCATCGCTTAAGTCGAG+GGG | 0.690101 | 3.1:+41202925 | None:intergenic |
| TTCAAAGCAGCATTGCAGAG+GGG | 0.703047 | 3.1:+41202886 | None:intergenic |
| ACAATACCGCCAGCACAACG+TGG | 0.758364 | 3.1:+41202949 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GCTTTAAAAGTAATGTAATA+TGG | + | chr3.1:41203304-41203323 | None:intergenic | 20.0% |
| ! | ACTTTATCAAACACAAAAGT+TGG | + | chr3.1:41203235-41203254 | None:intergenic | 25.0% |
| ! | CAATCAAACAAATATTAAGC+AGG | - | chr3.1:41203117-41203136 | MS.gene064562:CDS | 25.0% |
| !!! | CACTTAATTTTGATTGATTC+TGG | - | chr3.1:41202959-41202978 | MS.gene064562:CDS | 25.0% |
| AAAGTAATGTAATATGGCCT+AGG | + | chr3.1:41203298-41203317 | None:intergenic | 30.0% | |
| CAAACAAATATTAAGCAGGT+TGG | - | chr3.1:41203121-41203140 | MS.gene064562:CDS | 30.0% | |
| TGGTTCTTTGTATACGAATA+AGG | - | chr3.1:41202824-41202843 | MS.gene064562:intron | 30.0% | |
| ! | ACAATGACAGTTTTCCATTT+TGG | - | chr3.1:41202787-41202806 | MS.gene064562:intron | 30.0% |
| AAAGCAAAAGCAGAAGCAAA+GGG | - | chr3.1:41203319-41203338 | MS.gene064562:CDS | 35.0% | |
| ACACGATGAAGATTCATATG+AGG | - | chr3.1:41202620-41202639 | MS.gene064562:CDS | 35.0% | |
| AGAAGATTCTGAGTATAGTG+AGG | - | chr3.1:41202737-41202756 | MS.gene064562:intron | 35.0% | |
| AGCAAACTAAGTGTAAGTGT+AGG | - | chr3.1:41202894-41202913 | MS.gene064562:CDS | 35.0% | |
| ATGAAACACTCTTTGACAAC+AGG | + | chr3.1:41203403-41203422 | None:intergenic | 35.0% | |
| CATGAAACAAGATTCATCTG+TGG | - | chr3.1:41202665-41202684 | MS.gene064562:CDS | 35.0% | |
| CTACTTCTTTATATGCTCCT+AGG | - | chr3.1:41203278-41203297 | MS.gene064562:CDS | 35.0% | |
| CTTTGTATACGAATAAGGCT+TGG | - | chr3.1:41202829-41202848 | MS.gene064562:intron | 35.0% | |
| TAAAGCAAAAGCAGAAGCAA+AGG | - | chr3.1:41203318-41203337 | MS.gene064562:CDS | 35.0% | |
| TCACTATACTCAGAATCTTC+TGG | + | chr3.1:41202738-41202757 | None:intergenic | 35.0% | |
| TGAAGATTCATATGAGGATG+AGG | - | chr3.1:41202626-41202645 | MS.gene064562:CDS | 35.0% | |
| TGGACAATACAATACAAAGC+AGG | - | chr3.1:41202979-41202998 | MS.gene064562:intron | 35.0% | |
| AAATCGAAACGGCTGAAATC+GGG | - | chr3.1:41202585-41202604 | MS.gene064562:CDS | 40.0% | |
| AACAGGAACCGAAATCGAAA+CGG | - | chr3.1:41202574-41202593 | MS.gene064562:CDS | 40.0% | |
| AGATTCATATGAGGATGAGG+AGG | - | chr3.1:41202629-41202648 | MS.gene064562:CDS | 40.0% | |
| ATAAAGAAGTAGCACCAGGA+TGG | + | chr3.1:41203270-41203289 | None:intergenic | 40.0% | |
| GAAACAAGATTCATCTGTGG+AGG | - | chr3.1:41202668-41202687 | MS.gene064562:CDS | 40.0% | |
| GAAATCGAAACGGCTGAAAT+CGG | - | chr3.1:41202584-41202603 | MS.gene064562:CDS | 40.0% | |
| GATTTCAGCCGTTTCGATTT+CGG | + | chr3.1:41202585-41202604 | None:intergenic | 40.0% | |
| GCATATAAAGAAGTAGCACC+AGG | + | chr3.1:41203274-41203293 | None:intergenic | 40.0% | |
| GTATACGAATAAGGCTTGGA+AGG | - | chr3.1:41202833-41202852 | MS.gene064562:intron | 40.0% | |
| TAAAGAAGTAGCACCAGGAT+GGG | + | chr3.1:41203269-41203288 | None:intergenic | 40.0% | |
| TACTCAGAATCTTCTGGATC+CGG | + | chr3.1:41202732-41202751 | None:intergenic | 40.0% | |
| TCACGACGCTTATACCAAAA+TGG | + | chr3.1:41202804-41202823 | None:intergenic | 40.0% | |
| !! | TTTTGGTATAAGCGTCGTGA+TGG | - | chr3.1:41202804-41202823 | MS.gene064562:intron | 40.0% |
| ACAAGATTCATCTGTGGAGG+AGG | - | chr3.1:41202671-41202690 | MS.gene064562:CDS | 45.0% | |
| ACATCATCGCTTAAGTCGAG+GGG | + | chr3.1:41203052-41203071 | None:intergenic | 45.0% | |
| CACATCATCGCTTAAGTCGA+GGG | + | chr3.1:41203053-41203072 | None:intergenic | 45.0% | |
| CTTCAAAGCAGCATTGCAGA+GGG | + | chr3.1:41203092-41203111 | None:intergenic | 45.0% | |
| GAATCTTCTGGATCCGGTTT+CGG | + | chr3.1:41202726-41202745 | None:intergenic | 45.0% | |
| GTCTGGAAAGTTGTTAGAGC+AGG | + | chr3.1:41203352-41203371 | None:intergenic | 45.0% | |
| TAAGGCTTGGAAGGATCGTA+AGG | - | chr3.1:41202842-41202861 | MS.gene064562:intron | 45.0% | |
| TACGCTGCAATACATGAGTC+TGG | + | chr3.1:41203369-41203388 | None:intergenic | 45.0% | |
| TCTTCAAAGCAGCATTGCAG+AGG | + | chr3.1:41203093-41203112 | None:intergenic | 45.0% | |
| TGAGTATAGTGAGGAAGACG+AGG | - | chr3.1:41202746-41202765 | MS.gene064562:intron | 45.0% | |
| TTCAAAGCAGCATTGCAGAG+GGG | + | chr3.1:41203091-41203110 | None:intergenic | 45.0% | |
| !! | TACTTATTTATTATTAATTA+GGG | - | chr3.1:41203208-41203227 | MS.gene064562:CDS | 5.0% |
| !! | TTACTTATTTATTATTAATT+AGG | - | chr3.1:41203207-41203226 | MS.gene064562:CDS | 5.0% |
| AGATTCATCTGTGGAGGAGG+AGG | - | chr3.1:41202674-41202693 | MS.gene064562:CDS | 50.0% | |
| GAAACGGCTGAAATCGGGAT+CGG | - | chr3.1:41202590-41202609 | MS.gene064562:CDS | 50.0% | |
| GCAAAAGCAGAAGCAAAGGG+AGG | - | chr3.1:41203322-41203341 | MS.gene064562:CDS | 50.0% | |
| GCACATCATCGCTTAAGTCG+AGG | + | chr3.1:41203054-41203073 | None:intergenic | 50.0% | |
| GCTGAAATCGGAACCGAAAC+CGG | - | chr3.1:41202710-41202729 | MS.gene064562:CDS | 50.0% | |
| GGAGGAGAAACGGCTGAAAT+CGG | - | chr3.1:41202698-41202717 | MS.gene064562:CDS | 50.0% | |
| GTTGTCAAGTGTACCCATCC+TGG | - | chr3.1:41203253-41203272 | MS.gene064562:CDS | 50.0% | |
| TGAGGATGAGGAGGATAAGC+AGG | - | chr3.1:41202638-41202657 | MS.gene064562:CDS | 50.0% | |
| TGCAGAGGGGTTTGAGAGTA+CGG | + | chr3.1:41203078-41203097 | None:intergenic | 50.0% | |
| ACAATACCGCCAGCACAACG+TGG | + | chr3.1:41203028-41203047 | None:intergenic | 55.0% | |
| AGGAGGAGGAGGAGGAGAAA+CGG | - | chr3.1:41202688-41202707 | MS.gene064562:CDS | 55.0% | |
| TTCATCTGTGGAGGAGGAGG+AGG | - | chr3.1:41202677-41202696 | MS.gene064562:CDS | 55.0% | |
| ATACCGCCAGCACAACGTGG+TGG | + | chr3.1:41203025-41203044 | None:intergenic | 60.0% | |
| ATCTGTGGAGGAGGAGGAGG+AGG | - | chr3.1:41202680-41202699 | MS.gene064562:CDS | 60.0% | |
| CACTGTCCACCACGTTGTGC+TGG | - | chr3.1:41203016-41203035 | MS.gene064562:intron | 60.0% | |
| TGTCCACCACGTTGTGCTGG+CGG | - | chr3.1:41203019-41203038 | MS.gene064562:intron | 60.0% | |
| !! | CAGCACAACGTGGTGGACAG+TGG | + | chr3.1:41203018-41203037 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 41202561 | 41203435 | 41202561 | ID=MS.gene064562 |
| chr3.1 | mRNA | 41202561 | 41203435 | 41202561 | ID=MS.gene064562.t1;Parent=MS.gene064562 |
| chr3.1 | exon | 41203091 | 41203435 | 41203091 | ID=MS.gene064562.t1.exon1;Parent=MS.gene064562.t1 |
| chr3.1 | CDS | 41203091 | 41203435 | 41203091 | ID=cds.MS.gene064562.t1;Parent=MS.gene064562.t1 |
| chr3.1 | exon | 41202858 | 41202995 | 41202858 | ID=MS.gene064562.t1.exon2;Parent=MS.gene064562.t1 |
| chr3.1 | CDS | 41202858 | 41202995 | 41202858 | ID=cds.MS.gene064562.t1;Parent=MS.gene064562.t1 |
| chr3.1 | exon | 41202561 | 41202734 | 41202561 | ID=MS.gene064562.t1.exon3;Parent=MS.gene064562.t1 |
| chr3.1 | CDS | 41202561 | 41202734 | 41202561 | ID=cds.MS.gene064562.t1;Parent=MS.gene064562.t1 |
| Gene Sequence |
| Protein sequence |
