AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059830.t1 MTR_1g092430 85.437 103 10 1 1 103 1 98 4.36e-45 141
MS.gene059830.t1 MTR_0271s0050 74.038 104 11 2 1 103 1 89 5.58e-39 125
MS.gene059830.t1 MTR_0648s0010 74.038 104 11 2 1 103 1 89 5.58e-39 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059830.t1 AT3G15352 88.889 45 5 0 59 103 53 97 6.22e-27 95.5
MS.gene059830.t1 AT3G15352 88.889 45 5 0 59 103 31 75 6.59e-27 94.7
MS.gene059830.t1 AT3G15352 88.889 45 5 0 59 103 30 74 7.31e-27 94.4
MS.gene059830.t1 AT3G15352 88.889 45 5 0 59 103 37 81 1.08e-26 94.4
MS.gene059830.t1 AT1G53030 82.222 45 8 0 59 103 28 72 1.65e-24 88.6

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCGGCGTTGCTGCAGTTGC+TGG 0.322432 2.4:-18345584 None:intergenic
GCTGGCGTTGCTGCTGTTGA+TGG 0.363493 2.4:-18345611 None:intergenic
ACCAGCAACTGCAGCAACGC+CGG 0.403671 2.4:+18345583 MS.gene059830:CDS
AACGCCAGCGACGGCAGAAA+CGG 0.425755 2.4:+18345625 MS.gene059830:CDS
TCTCGCAGCCTCTTCGTGTC+AGG 0.437710 2.4:-18345713 None:intergenic
CAGTTGCTGGTGCTGTTGGT+TGG 0.439790 2.4:-18345571 None:intergenic
CGAATTTGTCTTCGTGCTGA+AGG 0.441593 2.4:+18345791 MS.gene059830:CDS
GCCGCCGTTTCTGCCGTCGC+TGG 0.445253 2.4:-18345629 None:intergenic
GCTGCTGTTGATGGCGCTGC+CGG 0.473729 2.4:-18345602 None:intergenic
GCTGCAGTTGCTGGTGCTGT+TGG 0.489129 2.4:-18345575 None:intergenic
AACAGCAGCAACGCCAGCGA+CGG 0.494709 2.4:+18345616 MS.gene059830:CDS
TTTGACACTTCACATGCTGC+TGG 0.518991 2.4:-18345656 None:intergenic
GCCAGCGACGGCAGAAACGG+CGG 0.552546 2.4:+18345628 MS.gene059830:CDS
TGGTTCGAAGCAGAGGAAGA+AGG 0.559341 2.4:-18345551 None:intergenic
TGTTGGTTGGTTCGAAGCAG+AGG 0.577421 2.4:-18345558 None:intergenic
GGTAACGCGCAAGTGCAAAA+CGG 0.577765 2.4:+18345518 MS.gene059830:CDS
ATGAGTGCATAGTGGAACAC+GGG 0.582328 2.4:+18345735 MS.gene059830:CDS
TCGATGAGCTTCAATCCACT+TGG 0.585020 2.4:-18345771 None:intergenic
GCGCTTGTCCTGACACGAAG+AGG 0.611666 2.4:+18345705 MS.gene059830:CDS
GCTGCGAGATGAGTGCATAG+TGG 0.621172 2.4:+18345727 MS.gene059830:CDS
GGGAGGATGCTTGTGCCAAG+TGG 0.626172 2.4:+18345756 MS.gene059830:CDS
GATGAGTGCATAGTGGAACA+CGG 0.674556 2.4:+18345734 MS.gene059830:CDS
GTGCATAGTGGAACACGGGG+AGG 0.723863 2.4:+18345739 MS.gene059830:CDS
TGAGTGCATAGTGGAACACG+GGG 0.769217 2.4:+18345736 MS.gene059830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAACAGATTTTCTTCTTCTT+CGG - chr2.4:18345686-18345705 None:intergenic 30.0%
CGAATTTGTCTTCGTGCTGA+AGG + chr2.4:18345791-18345810 MS.gene059830:CDS 45.0%
TCGATGAGCTTCAATCCACT+TGG - chr2.4:18345774-18345793 None:intergenic 45.0%
TTTGACACTTCACATGCTGC+TGG - chr2.4:18345659-18345678 None:intergenic 45.0%
! ATGAGTGCATAGTGGAACAC+GGG + chr2.4:18345735-18345754 MS.gene059830:CDS 45.0%
! GATGAGTGCATAGTGGAACA+CGG + chr2.4:18345734-18345753 MS.gene059830:CDS 45.0%
GGTAACGCGCAAGTGCAAAA+CGG + chr2.4:18345518-18345537 MS.gene059830:CDS 50.0%
TGGTTCGAAGCAGAGGAAGA+AGG - chr2.4:18345554-18345573 None:intergenic 50.0%
! TGAGTGCATAGTGGAACACG+GGG + chr2.4:18345736-18345755 MS.gene059830:CDS 50.0%
!! TGTTGGTTGGTTCGAAGCAG+AGG - chr2.4:18345561-18345580 None:intergenic 50.0%
GCTGCGAGATGAGTGCATAG+TGG + chr2.4:18345727-18345746 MS.gene059830:CDS 55.0%
!! CAGTTGCTGGTGCTGTTGGT+TGG - chr2.4:18345574-18345593 None:intergenic 55.0%
AACAGCAGCAACGCCAGCGA+CGG + chr2.4:18345616-18345635 MS.gene059830:CDS 60.0%
AACGCCAGCGACGGCAGAAA+CGG + chr2.4:18345625-18345644 MS.gene059830:CDS 60.0%
ACCAGCAACTGCAGCAACGC+CGG + chr2.4:18345583-18345602 MS.gene059830:CDS 60.0%
GCGCTTGTCCTGACACGAAG+AGG + chr2.4:18345705-18345724 MS.gene059830:CDS 60.0%
GCTGGCGTTGCTGCTGTTGA+TGG - chr2.4:18345614-18345633 None:intergenic 60.0%
GGGAGGATGCTTGTGCCAAG+TGG + chr2.4:18345756-18345775 MS.gene059830:CDS 60.0%
TCTCGCAGCCTCTTCGTGTC+AGG - chr2.4:18345716-18345735 None:intergenic 60.0%
! GTGCATAGTGGAACACGGGG+AGG + chr2.4:18345739-18345758 MS.gene059830:CDS 60.0%
!! GCTGCAGTTGCTGGTGCTGT+TGG - chr2.4:18345578-18345597 None:intergenic 60.0%
!! GCTGCTGTTGATGGCGCTGC+CGG - chr2.4:18345605-18345624 None:intergenic 65.0%
GCCAGCGACGGCAGAAACGG+CGG + chr2.4:18345628-18345647 MS.gene059830:CDS 70.0%
GCCGGCGTTGCTGCAGTTGC+TGG - chr2.4:18345587-18345606 None:intergenic 70.0%
GCCGCCGTTTCTGCCGTCGC+TGG - chr2.4:18345632-18345651 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 18345515 18345826 18345515 ID=MS.gene059830
chr2.4 mRNA 18345515 18345826 18345515 ID=MS.gene059830.t1;Parent=MS.gene059830
chr2.4 exon 18345515 18345826 18345515 ID=MS.gene059830.t1.exon1;Parent=MS.gene059830.t1
chr2.4 CDS 18345515 18345826 18345515 ID=cds.MS.gene059830.t1;Parent=MS.gene059830.t1
Gene Sequence

>MS.gene059830

ATGGGTAACGCGCAAGTGCAAAACGGTGTTGCTGTTCCTTCTTCCTCTGCTTCGAACCAACCAACAGCACCAGCAACTGCAGCAACGCCGGCAGCGCCATCAACAGCAGCAACGCCAGCGACGGCAGAAACGGCGGCAACGCCAGCAGCATGTGAAGTGTCAAATGCTCCGAAGAAGAAGAAAATCTGTTGCGCTTGTCCTGACACGAAGAGGCTGCGAGATGAGTGCATAGTGGAACACGGGGAGGATGCTTGTGCCAAGTGGATTGAAGCTCATCGAATTTGTCTTCGTGCTGAAGGATTCAATGTTTGA

Protein sequence

>MS.gene059830.t1

MGNAQVQNGVAVPSSSASNQPTAPATAATPAAPSTAATPATAETAATPAACEVSNAPKKKKICCACPDTKRLRDECIVEHGEDACAKWIEAHRICLRAEGFNV