AlfalfaGEDB | MS.gene064741

M. sativa cultivar XinJiangDaYe / MS.gene064741

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064741.t1	XP_013462204.1	100	106	0	0	1	106	1	106	9.40E-54	219.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064741.t1	O65695	73.1	104	28	0	3	106	4	107	1.6e-38	159.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064741.t1	A0A072V2H5	100.0	106	0	0	1	106	1	106	6.8e-54	219.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050128	MS.gene064741	0.824587	7.44E-54	-1.69E-46
MS.gene052078	MS.gene064741	0.822229	2.65E-53	-1.69E-46
MS.gene052178	MS.gene064741	0.854402	1.26E-61	-1.69E-46
MS.gene053557	MS.gene064741	0.804039	2.62E-49	-1.69E-46
MS.gene055277	MS.gene064741	0.837502	5.00E-57	-1.69E-46
MS.gene055497	MS.gene064741	-0.801035	1.09E-48	-1.69E-46
MS.gene055896	MS.gene064741	0.80063	1.32E-48	-1.69E-46
MS.gene058660	MS.gene064741	0.809726	1.64E-50	-1.69E-46
MS.gene059294	MS.gene064741	0.808095	3.66E-50	-1.69E-46
MS.gene059894	MS.gene064741	0.804656	1.95E-49	-1.69E-46
MS.gene060005	MS.gene064741	0.807234	5.58E-50	-1.69E-46
MS.gene061112	MS.gene064741	0.802606	5.18E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene064741	MS.gene003632	PPI
MS.gene062938	MS.gene064741	PPI
MS.gene23365	MS.gene064741	PPI
MS.gene50692	MS.gene064741	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064741.t1	MTR_3g113310	100.000	106	0	0	1	106	1	106	3.90e-77	222
MS.gene064741.t1	MTR_3g117610	72.222	108	27	2	1	105	1	108	3.25e-51	157
MS.gene064741.t1	MTR_5g021820	67.925	106	32	1	1	106	1	104	5.10e-51	156
MS.gene064741.t1	MTR_4g072190	72.222	108	20	2	1	106	1	100	2.12e-50	154
MS.gene064741.t1	MTR_8g076040	70.093	107	29	3	1	106	1	105	5.10e-48	149
MS.gene064741.t1	MTR_6g077850	58.947	95	38	1	12	105	4	98	6.52e-34	113
MS.gene064741.t1	MTR_4g072880	49.438	89	38	2	18	105	8	90	4.76e-28	98.2
MS.gene064741.t1	MTR_4g072500	63.636	66	23	1	41	105	24	89	6.11e-27	95.5
MS.gene064741.t1	MTR_4g072310	61.194	67	25	1	40	105	24	90	1.04e-26	94.7
MS.gene064741.t1	MTR_4g072640	62.121	66	24	1	42	106	25	90	1.04e-26	94.7
MS.gene064741.t1	MTR_4g072270	61.538	65	24	1	40	103	24	88	1.12e-26	94.7
MS.gene064741.t1	MTR_4g072530	62.121	66	24	1	41	105	24	89	8.14e-26	92.4
MS.gene064741.t1	MTR_4g072820	59.701	67	26	1	41	106	19	85	1.42e-25	91.7
MS.gene064741.t1	MTR_4g072220	58.209	67	27	1	40	105	24	90	2.17e-25	91.3
MS.gene064741.t1	MTR_4g072860	50.000	90	38	2	18	106	8	91	2.34e-25	91.3
MS.gene064741.t1	MTR_4g072250	59.701	67	25	2	40	105	24	89	2.99e-25	90.9
MS.gene064741.t1	MTR_4g072730	58.824	68	27	1	40	106	23	90	4.24e-25	90.5
MS.gene064741.t1	MTR_4g072480	58.209	67	27	1	40	105	23	89	5.06e-25	90.5
MS.gene064741.t1	MTR_8g461410	56.923	65	27	1	40	103	23	87	6.29e-25	90.1
MS.gene064741.t1	MTR_4g072910	62.121	66	24	1	41	105	34	99	6.56e-25	90.5
MS.gene064741.t1	MTR_4g072370	59.701	67	26	1	40	105	21	87	7.09e-25	90.1
MS.gene064741.t1	MTR_4g072260	58.209	67	27	1	40	105	24	90	7.21e-25	90.1
MS.gene064741.t1	MTR_4g072620	57.353	68	28	1	40	106	23	90	1.07e-24	89.7
MS.gene064741.t1	MTR_5g008360	55.882	68	29	1	40	106	14	81	1.26e-24	89.4
MS.gene064741.t1	MTR_4g072890	58.209	67	27	1	40	105	24	90	1.64e-24	89.0
MS.gene064741.t1	MTR_3g084150	56.923	65	27	1	40	103	23	87	1.82e-24	89.0
MS.gene064741.t1	MTR_3g084240	60.000	65	25	1	40	103	23	87	2.21e-24	89.0
MS.gene064741.t1	MTR_3g084180	56.923	65	27	1	40	103	23	87	2.41e-24	88.6
MS.gene064741.t1	MTR_3g084170	60.000	65	25	1	40	103	23	87	2.69e-24	88.6
MS.gene064741.t1	MTR_4g072870	48.913	92	40	3	15	105	5	90	3.08e-24	88.6
MS.gene064741.t1	MTR_4g072240	53.731	67	30	1	40	105	24	90	3.55e-24	88.2
MS.gene064741.t1	MTR_3g110038	58.462	65	26	1	40	103	23	87	4.70e-24	88.2
MS.gene064741.t1	MTR_3g084250	58.824	68	27	1	39	105	22	89	8.56e-24	87.4
MS.gene064741.t1	MTR_5g008370	58.209	67	27	1	41	106	24	90	8.71e-24	88.6
MS.gene064741.t1	MTR_4g072230	60.000	65	23	2	40	103	24	86	1.41e-23	86.7
MS.gene064741.t1	MTR_4g072570	55.224	67	29	1	40	105	23	89	1.51e-23	86.7
MS.gene064741.t1	MTR_4g072580	55.224	67	29	1	40	105	18	84	1.71e-23	86.3
MS.gene064741.t1	MTR_4g072660	56.923	65	27	1	40	103	18	82	1.87e-23	86.3
MS.gene064741.t1	MTR_4g072540	54.412	68	30	1	40	106	19	86	1.92e-23	86.3
MS.gene064741.t1	MTR_4g072330	56.061	66	28	1	41	105	22	87	2.07e-23	86.3
MS.gene064741.t1	MTR_4g072300	52.239	67	31	1	40	105	24	90	2.25e-23	86.3
MS.gene064741.t1	MTR_3g084210	58.824	68	27	1	39	105	22	89	2.91e-23	85.9
MS.gene064741.t1	MTR_4g072980	49.020	102	37	5	8	106	4	93	3.09e-23	85.9
MS.gene064741.t1	MTR_4g072560	56.716	67	27	2	41	105	31	97	3.30e-23	86.3
MS.gene064741.t1	MTR_4g072850	54.412	68	30	1	40	106	25	92	3.58e-23	85.9
MS.gene064741.t1	MTR_3g084220	60.000	65	25	1	40	103	23	87	4.46e-23	85.5
MS.gene064741.t1	MTR_3g117630	56.923	65	27	1	40	103	23	87	4.97e-23	85.5
MS.gene064741.t1	MTR_4g072610	56.716	67	28	1	40	105	17	83	6.83e-23	84.7
MS.gene064741.t1	MTR_4g072680	55.224	67	29	1	40	105	23	89	7.77e-23	85.1
MS.gene064741.t1	MTR_4g072550	57.812	64	26	1	41	103	35	98	9.61e-23	85.1
MS.gene064741.t1	MTR_4g072460	54.412	68	30	1	40	106	23	90	1.19e-22	84.3
MS.gene064741.t1	MTR_3g084160	55.882	68	29	1	39	105	22	89	1.40e-22	84.3
MS.gene064741.t1	MTR_3g084230	57.353	68	28	1	39	105	22	89	1.46e-22	84.3
MS.gene064741.t1	MTR_4g072590	52.941	68	31	1	40	106	18	85	3.03e-22	83.2
MS.gene064741.t1	MTR_4g072510	52.941	68	31	1	40	106	19	86	6.76e-22	82.4
MS.gene064741.t1	MTR_4g072830	53.731	67	30	1	40	105	21	87	6.83e-22	82.4
MS.gene064741.t1	MTR_4g072490	52.239	67	31	1	40	105	18	84	7.03e-22	82.4
MS.gene064741.t1	MTR_3g117640	58.209	67	26	2	41	105	33	99	7.26e-22	82.8
MS.gene064741.t1	MTR_4g072940	51.471	68	32	1	40	106	55	122	8.15e-22	83.2
MS.gene064741.t1	MTR_4g072740	53.731	67	30	1	40	105	18	84	8.37e-22	82.0
MS.gene064741.t1	MTR_8g461420	55.882	68	29	1	39	105	22	89	8.49e-22	82.4
MS.gene064741.t1	MTR_4g072670	53.731	67	30	1	40	105	19	85	8.69e-22	82.0
MS.gene064741.t1	MTR_3g084190	54.412	68	30	1	39	105	22	89	9.57e-22	82.0
MS.gene064741.t1	MTR_4g072720	51.471	68	32	1	40	106	19	86	1.09e-21	81.6
MS.gene064741.t1	MTR_4g050700	56.250	64	27	1	41	103	25	88	1.24e-21	82.0
MS.gene064741.t1	MTR_2g012410	56.250	64	27	1	41	103	25	88	1.24e-21	82.0
MS.gene064741.t1	MTR_3g110045	58.209	67	27	1	40	105	82	148	1.94e-21	83.2
MS.gene064741.t1	MTR_4g072600	53.731	67	30	1	40	105	18	84	2.21e-21	81.3
MS.gene064741.t1	MTR_4g072470	53.731	67	30	1	40	105	18	84	2.87e-21	80.9
MS.gene064741.t1	MTR_3g110042	56.667	60	25	1	40	98	23	82	4.62e-21	80.5
MS.gene064741.t1	MTR_4g072630	50.746	67	32	1	40	105	19	85	8.99e-21	79.3
MS.gene064741.t1	MTR_4g072930	46.078	102	43	3	14	103	6	107	1.37e-20	79.7
MS.gene064741.t1	MTR_4g072770	57.143	63	26	1	42	103	15	77	1.45e-20	79.0
MS.gene064741.t1	MTR_4g072650	52.239	67	31	1	40	105	17	83	1.49e-20	79.0
MS.gene064741.t1	MTR_4g072690	53.030	66	30	1	42	106	20	85	1.84e-20	78.6
MS.gene064741.t1	MTR_3g084260	53.125	64	29	1	41	103	25	88	2.96e-20	78.6
MS.gene064741.t1	MTR_4g072465	50.769	65	31	1	40	103	19	83	4.77e-20	77.8
MS.gene064741.t1	MTR_4g072380	53.125	64	29	1	41	103	20	83	1.96e-19	76.3
MS.gene064741.t1	MTR_3g110032	51.562	64	30	1	41	103	25	88	1.97e-19	76.3
MS.gene064741.t1	MTR_4g072450	50.746	67	32	1	40	105	18	84	3.94e-19	75.5
MS.gene064741.t1	MTR_1g063950	55.738	61	27	0	41	101	47	107	5.93e-19	75.9
MS.gene064741.t1	MTR_7g101740	53.125	64	30	0	41	104	39	102	3.03e-18	73.9
MS.gene064741.t1	MTR_1g094750	51.724	58	28	0	41	98	49	106	5.81e-17	70.5
MS.gene064741.t1	MTR_5g008350	49.123	57	28	1	40	95	18	74	7.62e-17	69.3
MS.gene064741.t1	MTR_1g024085	37.895	95	55	1	5	99	4	94	1.18e-16	70.5
MS.gene064741.t1	MTR_2g043960	52.459	61	29	0	41	101	75	135	2.52e-16	70.1
MS.gene064741.t1	MTR_4g072750	56.250	48	21	0	41	88	26	73	3.53e-16	67.8
MS.gene064741.t1	MTR_1g009150	41.573	89	51	1	17	105	47	134	4.57e-16	70.1
MS.gene064741.t1	MTR_3g084200	53.061	49	23	0	40	88	23	71	7.24e-16	67.4
MS.gene064741.t1	MTR_4g072290	53.846	52	23	1	55	105	25	76	8.81e-16	66.6
MS.gene064741.t1	MTR_4g124750	46.970	66	35	0	41	106	68	133	2.10e-15	67.8
MS.gene064741.t1	MTR_3g110035	55.357	56	24	1	51	105	80	135	2.80e-15	67.0
MS.gene064741.t1	MTR_3g109160	39.326	89	49	1	17	105	49	132	3.49e-15	67.4
MS.gene064741.t1	MTR_0388s0020	42.857	70	37	1	39	105	72	141	1.19e-14	65.5
MS.gene064741.t1	MTR_4g025830	38.298	94	46	3	22	105	54	145	1.47e-14	65.5
MS.gene064741.t1	MTR_8g461210	43.939	66	37	0	41	106	34	99	1.55e-14	64.3
MS.gene064741.t1	MTR_7g104950	44.262	61	34	0	45	105	53	113	4.29e-14	63.5
MS.gene064741.t1	MTR_7g118010	40.909	66	37	1	42	105	23	88	7.42e-14	62.4
MS.gene064741.t1	MTR_3g092220	38.356	73	43	1	35	105	26	98	7.46e-14	62.8
MS.gene064741.t1	MTR_7g104940	41.935	62	36	0	45	106	53	114	1.00e-13	62.8
MS.gene064741.t1	MTR_4g072280	52.273	44	21	0	40	83	24	67	1.33e-13	61.2
MS.gene064741.t1	MTR_1g059770	38.462	65	40	0	42	106	49	113	2.73e-13	61.6
MS.gene064741.t1	MTR_1g061570	42.623	61	35	0	41	101	18	78	3.27e-13	61.2
MS.gene064741.t1	MTR_3g098970	40.000	65	39	0	34	98	45	109	6.45e-13	61.2
MS.gene064741.t1	MTR_4g005310	32.000	100	57	2	4	101	33	123	6.74e-13	61.2
MS.gene064741.t1	MTR_4g072160	43.077	65	37	0	41	105	36	100	6.83e-13	60.8
MS.gene064741.t1	MTR_4g005320	42.188	64	37	0	42	105	70	133	1.29e-12	60.5
MS.gene064741.t1	MTR_2g095430	45.614	57	27	1	41	93	44	100	2.51e-12	59.3
MS.gene064741.t1	MTR_8g026730	39.394	66	38	1	42	105	27	92	3.80e-12	58.2
MS.gene064741.t1	MTR_4g057800	50.877	57	24	1	41	93	37	93	4.50e-12	58.5
MS.gene064741.t1	MTR_4g057810	41.270	63	35	1	41	101	37	99	8.75e-12	57.4
MS.gene064741.t1	MTR_8g101520	30.000	110	71	1	2	105	4	113	1.15e-11	58.2
MS.gene064741.t1	MTR_8g096470	41.071	56	33	0	43	98	47	102	9.81e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064741.t1	AT1G75580	75.000	108	25	1	1	106	1	108	2.89e-55	167
MS.gene064741.t1	AT4G34760	73.832	107	27	1	1	106	1	107	1.16e-53	163
MS.gene064741.t1	AT2G21220	69.524	105	30	1	1	105	1	103	8.12e-50	153
MS.gene064741.t1	AT4G38860	71.963	107	27	2	1	106	1	105	3.68e-49	152
MS.gene064741.t1	AT1G19830	69.027	113	28	3	1	106	1	113	8.62e-48	149
MS.gene064741.t1	AT2G16580	68.571	105	32	1	3	106	4	108	1.17e-46	145
MS.gene064741.t1	AT4G36110	64.815	108	32	2	1	106	1	104	6.88e-43	135
MS.gene064741.t1	AT2G18010	61.607	112	37	2	1	106	1	112	9.29e-40	128
MS.gene064741.t1	AT5G66260	59.341	91	33	1	15	105	11	97	1.66e-32	109
MS.gene064741.t1	AT4G34770	60.294	68	24	2	41	105	34	101	3.36e-24	89.0
MS.gene064741.t1	AT2G21210	47.222	108	40	4	1	105	1	94	1.48e-23	87.0
MS.gene064741.t1	AT4G38840	60.000	65	24	2	41	103	32	96	1.94e-23	86.7
MS.gene064741.t1	AT2G21210	47.222	108	40	4	1	105	68	161	5.69e-23	87.4
MS.gene064741.t1	AT5G18030	58.209	67	26	2	42	106	22	88	1.19e-22	84.3
MS.gene064741.t1	AT2G21200	58.209	67	26	2	41	105	20	86	2.25e-22	83.6
MS.gene064741.t1	AT5G18080	46.237	93	35	3	16	106	11	90	3.37e-22	83.2
MS.gene064741.t1	AT3G51200	41.121	107	49	3	1	101	1	99	5.91e-22	83.2
MS.gene064741.t1	AT5G18010	46.237	93	35	3	16	106	11	90	6.01e-22	82.8
MS.gene064741.t1	AT5G18020	59.375	64	24	2	42	103	24	87	7.19e-22	82.4
MS.gene064741.t1	AT4G13790	52.857	70	30	2	40	106	23	92	8.51e-22	82.4
MS.gene064741.t1	AT5G18060	58.462	65	25	2	43	105	25	89	1.01e-21	82.0
MS.gene064741.t1	AT5G18050	55.224	67	28	2	42	106	24	90	1.06e-21	82.0
MS.gene064741.t1	AT4G38825	54.286	70	30	2	38	105	19	88	3.86e-21	80.5
MS.gene064741.t1	AT4G38850	43.158	95	34	3	16	106	11	89	1.26e-20	79.3
MS.gene064741.t1	AT3G03840	53.623	69	29	2	41	106	26	94	1.87e-20	79.0
MS.gene064741.t1	AT4G34810	47.917	96	35	5	16	103	26	114	2.34e-20	79.3
MS.gene064741.t1	AT3G03850	56.716	67	26	2	43	106	26	92	2.66e-20	78.6
MS.gene064741.t1	AT4G34800	56.250	64	24	2	42	101	21	84	5.67e-20	77.8
MS.gene064741.t1	AT3G03820	51.429	70	30	2	41	106	26	95	9.65e-20	77.4
MS.gene064741.t1	AT4G34780	53.521	71	29	2	40	106	26	96	1.33e-19	77.0
MS.gene064741.t1	AT3G03830	53.623	69	28	2	42	106	24	92	3.18e-19	75.9
MS.gene064741.t1	AT3G20220	47.541	61	31	1	42	101	47	107	5.12e-18	73.6
MS.gene064741.t1	AT4G34750	38.614	101	59	1	5	105	3	100	2.21e-17	72.8
MS.gene064741.t1	AT4G34750	38.614	101	59	1	5	105	3	100	2.21e-17	72.8
MS.gene064741.t1	AT3G43120	54.098	61	28	0	41	101	78	138	2.75e-17	72.8
MS.gene064741.t1	AT2G37030	53.448	58	27	0	41	98	48	105	3.29e-17	71.6
MS.gene064741.t1	AT4G34790	52.308	65	28	2	42	103	39	103	7.38e-17	70.1
MS.gene064741.t1	AT1G75590	37.895	95	57	1	5	99	7	99	1.12e-16	70.9
MS.gene064741.t1	AT5G20810	52.459	61	29	0	41	101	78	138	1.70e-16	70.9
MS.gene064741.t1	AT5G20810	52.459	61	29	0	41	101	78	138	3.27e-16	70.5
MS.gene064741.t1	AT5G10990	50.847	59	29	0	41	99	40	98	3.80e-15	67.0
MS.gene064741.t1	AT1G19840	37.895	95	56	1	5	99	7	98	1.25e-14	65.5
MS.gene064741.t1	AT3G61900	41.096	73	39	2	36	105	25	96	2.31e-14	64.7
MS.gene064741.t1	AT4G09530	44.118	68	34	2	42	105	28	95	4.85e-14	62.8
MS.gene064741.t1	AT2G24400	46.875	64	34	0	42	105	64	127	7.40e-14	64.3
MS.gene064741.t1	AT5G53590	44.286	70	34	1	41	105	45	114	1.40e-13	62.8
MS.gene064741.t1	AT2G45210	41.791	67	36	1	42	105	79	145	1.44e-13	63.2
MS.gene064741.t1	AT4G31320	45.312	64	35	0	42	105	80	143	2.09e-13	63.2
MS.gene064741.t1	AT3G60690	43.284	67	35	1	42	105	88	154	2.15e-13	62.8
MS.gene064741.t1	AT2G46690	39.706	68	38	1	41	105	23	90	1.49e-12	59.7
MS.gene064741.t1	AT1G56150	32.353	102	59	1	15	106	1	102	2.23e-12	58.9
MS.gene064741.t1	AT2G28085	48.276	58	26	1	41	94	54	111	3.20e-12	58.9
MS.gene064741.t1	AT1G79130	41.538	65	38	0	42	106	48	112	1.00e-11	57.8
MS.gene064741.t1	AT3G12830	40.000	65	39	0	42	106	50	114	1.82e-11	57.0
MS.gene064741.t1	AT4G12410	44.444	63	32	1	42	101	76	138	2.90e-11	57.0
MS.gene064741.t1	AT3G53250	35.385	65	39	1	34	98	44	105	3.31e-11	56.2
MS.gene064741.t1	AT4G00880	39.394	66	38	1	42	105	28	93	7.03e-11	55.1
MS.gene064741.t1	AT1G76190	38.095	63	38	1	44	106	23	84	7.41e-11	55.1

Find 17 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAAGCAGAAGAAGAGTT+TGG	0.272639	3.4:-97726783	MS.gene064741:CDS
TATACAACAAAGTGACCTTT+TGG	0.315026	3.4:+97726879	None:intergenic
TGATCATCATGGTCTTCGTT+TGG	0.370411	3.4:-97726907	MS.gene064741:CDS
ATCAAGCAGAAGAAGAGTTT+GGG	0.391525	3.4:-97726782	MS.gene064741:CDS
CTTCTTCTGCTTGATGAAGA+AGG	0.410523	3.4:+97726790	None:intergenic
GGTCTACTTAGAAAAGCAAT+AGG	0.458823	3.4:+97726825	None:intergenic
CTTAAAAGATGTTCAAGTCT+AGG	0.465781	3.4:-97726951	MS.gene064741:CDS
CTTCGTTTGGATGTTCCAAA+AGG	0.478320	3.4:-97726894	MS.gene064741:CDS
AACATCTTGGTTGCATGGAA+TGG	0.497565	3.4:+97726737	None:intergenic
GGTCACTTTGTTGTATATGT+TGG	0.526002	3.4:-97726873	MS.gene064741:CDS
TGTTTGATGAGTGCTGTTTG+AGG	0.534488	3.4:+97726978	None:intergenic
CAAGGGTACGTTGATCATCA+TGG	0.559732	3.4:-97726918	MS.gene064741:CDS
GTTTGGGTTTGATCATGACA+TGG	0.586043	3.4:-97726766	MS.gene064741:CDS
TTTGGGTTTGATCATGACAT+GGG	0.603751	3.4:-97726765	MS.gene064741:CDS
ATGTTGGTGAGAACAGAAGC+AGG	0.613401	3.4:-97726857	MS.gene064741:CDS
AGTCTAGGCAAGAAACAACA+AGG	0.622463	3.4:-97726936	MS.gene064741:CDS
GTCTAGGCAAGAAACAACAA+GGG	0.721725	3.4:-97726935	MS.gene064741:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTTAAAAGATGTTCAAGTCT+AGG	-	chr3.4:97726761-97726780	MS.gene064741:CDS	30.0%
	TAAGAGACTCAAAAACATCT+TGG	+	chr3.4:97726991-97727010	None:intergenic	30.0%
!	CTAGACTTGAACATCTTTTA+AGG	+	chr3.4:97726763-97726782	None:intergenic	30.0%
!	TATACAACAAAGTGACCTTT+TGG	+	chr3.4:97726836-97726855	None:intergenic	30.0%
	ATCAAGCAGAAGAAGAGTTT+GGG	-	chr3.4:97726930-97726949	MS.gene064741:CDS	35.0%
	GGTCTACTTAGAAAAGCAAT+AGG	+	chr3.4:97726890-97726909	None:intergenic	35.0%
	TCAAAAACATCTTGGTTGCA+TGG	+	chr3.4:97726983-97727002	None:intergenic	35.0%
!	GGTCACTTTGTTGTATATGT+TGG	-	chr3.4:97726839-97726858	MS.gene064741:CDS	35.0%
!	TTTGGGTTTGATCATGACAT+GGG	-	chr3.4:97726947-97726966	MS.gene064741:CDS	35.0%
!!!	TGAAGAAGGTTTTGAAACTC+TGG	+	chr3.4:97726911-97726930	None:intergenic	35.0%
	AACATCTTGGTTGCATGGAA+TGG	+	chr3.4:97726978-97726997	None:intergenic	40.0%
	AGTCTAGGCAAGAAACAACA+AGG	-	chr3.4:97726776-97726795	MS.gene064741:CDS	40.0%
	CATCAAGCAGAAGAAGAGTT+TGG	-	chr3.4:97726929-97726948	MS.gene064741:CDS	40.0%
	CTTCGTTTGGATGTTCCAAA+AGG	-	chr3.4:97726818-97726837	MS.gene064741:CDS	40.0%
	CTTCTTCTGCTTGATGAAGA+AGG	+	chr3.4:97726925-97726944	None:intergenic	40.0%
	GTCTAGGCAAGAAACAACAA+GGG	-	chr3.4:97726777-97726796	MS.gene064741:CDS	40.0%
	TGATCATCATGGTCTTCGTT+TGG	-	chr3.4:97726805-97726824	MS.gene064741:CDS	40.0%
!	GTTTGGGTTTGATCATGACA+TGG	-	chr3.4:97726946-97726965	MS.gene064741:CDS	40.0%
!!	TGTTTGATGAGTGCTGTTTG+AGG	+	chr3.4:97726737-97726756	None:intergenic	40.0%
!	ATGTTGGTGAGAACAGAAGC+AGG	-	chr3.4:97726855-97726874	MS.gene064741:CDS	45.0%
!	CAAGGGTACGTTGATCATCA+TGG	-	chr3.4:97726794-97726813	MS.gene064741:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	97726707	97727027	97726707	ID=MS.gene064741
chr3.4	mRNA	97726707	97727027	97726707	ID=MS.gene064741.t1;Parent=MS.gene064741
chr3.4	exon	97726707	97727027	97726707	ID=MS.gene064741.t1.exon1;Parent=MS.gene064741.t1
chr3.4	CDS	97726707	97727027	97726707	ID=cds.MS.gene064741.t1;Parent=MS.gene064741.t1

Gene Sequence	>MS.gene064741 ATGGCTATAAGAAAATCAACAAAACTTCCTCAAACAGCACTCATCAAACAAATCCTTAAAAGATGTTCAAGTCTAGGCAAGAAACAACAAGGGTACGTTGATCATCATGGTCTTCGTTTGGATGTTCCAAAAGGTCACTTTGTTGTATATGTTGGTGAGAACAGAAGCAGGTACATTGTTCCTATTGCTTTTCTAAGTAGACCAGAGTTTCAAAACCTTCTTCATCAAGCAGAAGAAGAGTTTGGGTTTGATCATGACATGGGTCTTACCATTCCATGCAACCAAGATGTTTTTGAGTCTCTTACTTCAATGCTAAGATAG
Protein sequence	>MS.gene064741.t1 MAIRKSTKLPQTALIKQILKRCSSLGKKQQGYVDHHGLRLDVPKGHFVVYVGENRSRYIVPIAFLSRPEFQNLLHQAEEEFGFDHDMGLTIPCNQDVFESLTSMLR