AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065049

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065049.t1 MTR_1g112210 95.833 360 15 0 19 378 23 382 0.0 734
MS.gene065049.t1 MTR_1g112210 92.500 360 14 1 19 378 23 369 0.0 699
MS.gene065049.t1 MTR_1g050448 73.351 379 96 3 1 378 1 375 0.0 601
MS.gene065049.t1 MTR_3g464570 42.216 334 175 5 55 374 43 372 6.48e-99 298
MS.gene065049.t1 MTR_5g071350 40.469 341 179 5 55 374 58 395 1.83e-98 298
MS.gene065049.t1 MTR_2g015720 42.296 331 170 6 55 378 42 358 1.70e-95 289
MS.gene065049.t1 MTR_4g016940 42.075 347 177 8 53 378 84 427 1.57e-92 284
MS.gene065049.t1 MTR_3g049330 42.216 334 169 7 55 378 31 350 3.60e-92 280
MS.gene065049.t1 MTR_2g091175 39.458 332 180 6 54 378 49 366 1.44e-91 280
MS.gene065049.t1 MTR_7g093450 38.947 380 208 8 19 378 212 587 1.88e-89 281
MS.gene065049.t1 MTR_2g073500 42.687 335 167 8 53 378 27 345 2.62e-89 273
MS.gene065049.t1 MTR_0136s0010 41.210 347 184 7 50 378 165 509 3.85e-88 276
MS.gene065049.t1 MTR_2g073510 39.339 333 181 6 51 374 29 349 2.04e-87 268
MS.gene065049.t1 MTR_5g034810 40.719 334 176 7 53 378 33 352 1.17e-86 266
MS.gene065049.t1 MTR_7g109550 37.015 335 188 5 55 374 137 463 7.46e-85 266
MS.gene065049.t1 MTR_4g079700 37.725 334 181 8 55 378 47 363 9.07e-85 262
MS.gene065049.t1 MTR_7g068920 40.058 347 184 8 53 378 78 421 1.04e-84 264
MS.gene065049.t1 MTR_5g033800 37.260 365 206 9 30 378 126 483 1.91e-84 265
MS.gene065049.t1 MTR_5g059400 37.662 385 215 8 17 378 133 515 3.90e-84 265
MS.gene065049.t1 MTR_1g116130 37.662 385 215 8 17 378 133 515 3.90e-84 265
MS.gene065049.t1 MTR_3g070900 40.517 348 171 10 55 374 106 445 5.40e-83 260
MS.gene065049.t1 MTR_8g463790 39.595 346 180 10 55 378 71 409 1.88e-82 258
MS.gene065049.t1 MTR_4g087730 37.234 376 206 10 12 378 8 362 2.97e-82 255
MS.gene065049.t1 MTR_4g088030 36.556 331 190 6 55 378 33 350 6.09e-82 254
MS.gene065049.t1 MTR_4g014440 37.037 351 199 9 50 378 57 407 3.18e-81 254
MS.gene065049.t1 MTR_4g126950 34.896 384 220 8 21 378 67 446 1.12e-80 254
MS.gene065049.t1 MTR_5g082880 40.227 353 172 11 53 374 163 507 7.40e-80 254
MS.gene065049.t1 MTR_3g021090 35.127 353 206 7 30 374 7 344 1.18e-78 248
MS.gene065049.t1 MTR_3g035830 35.211 355 207 7 30 376 7 346 1.50e-78 245
MS.gene065049.t1 MTR_3g071650 35.969 392 222 10 14 378 41 430 3.51e-78 247
MS.gene065049.t1 MTR_1g111010 40.361 332 172 12 53 378 30 341 4.65e-78 244
MS.gene065049.t1 MTR_4g121560 35.263 380 220 8 21 378 78 453 5.10e-78 248
MS.gene065049.t1 MTR_3g021060 34.473 351 197 7 34 376 36 361 5.05e-76 239
MS.gene065049.t1 MTR_5g083260 37.971 345 190 9 55 378 83 424 6.24e-76 241
MS.gene065049.t1 MTR_8g069790 35.988 339 200 7 52 374 175 512 3.22e-75 242
MS.gene065049.t1 MTR_3g464570 41.111 270 142 4 118 374 3 268 6.12e-74 231
MS.gene065049.t1 MTR_7g070060 34.870 347 204 9 54 378 63 409 8.65e-74 235
MS.gene065049.t1 MTR_4g014430 35.244 349 207 7 47 378 100 446 8.37e-73 234
MS.gene065049.t1 MTR_1g058380 34.243 403 231 10 1 378 1 394 3.21e-71 228
MS.gene065049.t1 MTR_7g094750 35.260 346 198 8 55 378 91 432 5.14e-67 219
MS.gene065049.t1 MTR_2g022560 34.054 370 208 9 32 376 77 435 9.09e-67 218
MS.gene065049.t1 MTR_3g090120 35.638 376 204 9 25 378 100 459 3.66e-63 209
MS.gene065049.t1 MTR_8g093530 33.724 341 199 6 53 378 88 416 1.01e-61 204
MS.gene065049.t1 MTR_5g033800 37.165 261 147 6 30 279 126 380 4.03e-57 191
MS.gene065049.t1 MTR_7g070060 37.021 235 139 5 54 279 63 297 6.15e-54 181
MS.gene065049.t1 MTR_7g070060 31.741 293 180 7 106 378 15 307 6.05e-51 173
MS.gene065049.t1 MTR_7g094750 34.454 238 143 3 55 279 91 328 3.26e-48 166
MS.gene065049.t1 MTR_6g061710 29.459 370 215 12 40 374 152 510 2.53e-44 160
MS.gene065049.t1 MTR_1g076690 30.110 362 193 15 55 374 223 566 2.41e-40 150
MS.gene065049.t1 MTR_5g069320 30.466 279 152 6 98 371 145 386 1.61e-39 145
MS.gene065049.t1 MTR_8g027900 27.596 366 224 13 40 374 196 551 5.58e-36 138
MS.gene065049.t1 MTR_8g093870 28.451 355 208 15 53 376 131 470 8.76e-36 136
MS.gene065049.t1 MTR_7g019120 29.745 353 208 12 51 374 75 416 2.66e-35 134
MS.gene065049.t1 MTR_5g035090 27.596 366 212 13 46 374 74 423 4.40e-35 134
MS.gene065049.t1 MTR_7g033230 28.611 360 203 13 53 374 262 605 9.71e-35 135
MS.gene065049.t1 MTR_8g099300 27.020 396 246 14 11 374 31 415 1.68e-34 132
MS.gene065049.t1 MTR_7g033230 28.611 360 203 13 53 374 332 675 2.56e-34 134
MS.gene065049.t1 MTR_3g109840 28.169 355 204 13 55 374 73 411 1.47e-33 129
MS.gene065049.t1 MTR_8g104970 27.869 366 212 15 46 374 111 461 2.11e-33 130
MS.gene065049.t1 MTR_6g016880 26.161 409 251 14 7 374 17 415 4.68e-33 128
MS.gene065049.t1 MTR_4g092830 27.920 351 214 11 55 375 48 389 5.14e-33 128
MS.gene065049.t1 MTR_6g007777 27.901 362 207 15 53 378 114 457 7.15e-33 128
MS.gene065049.t1 MTR_5g035150 26.776 366 209 13 50 374 80 427 2.95e-32 126
MS.gene065049.t1 MTR_2g016060 28.652 356 196 13 59 374 59 396 9.52e-32 124
MS.gene065049.t1 MTR_5g035080 27.451 357 207 13 55 374 75 416 9.24e-31 122
MS.gene065049.t1 MTR_4g125060 27.933 358 212 15 52 378 168 510 9.95e-31 122
MS.gene065049.t1 MTR_4g125060 27.642 369 215 16 45 376 116 469 1.04e-30 122
MS.gene065049.t1 MTR_4g125060 27.933 358 212 15 52 378 197 539 1.29e-30 122
MS.gene065049.t1 MTR_5g035070 29.944 354 203 13 55 374 87 429 1.47e-30 121
MS.gene065049.t1 MTR_6g016875 27.042 355 214 10 53 374 62 404 2.20e-29 118
MS.gene065049.t1 MTR_6g016240 26.764 411 237 16 8 374 22 412 1.25e-25 107
MS.gene065049.t1 MTR_7g019140 46.875 96 50 1 51 145 75 170 1.54e-23 96.3
MS.gene065049.t1 MTR_5g035120 39.216 102 61 1 44 144 64 165 9.00e-20 86.3
MS.gene065049.t1 MTR_3g021150 33.645 107 64 2 240 339 1 107 2.34e-16 75.1
MS.gene065049.t1 MTR_2g022580 35.593 118 64 3 115 220 3 120 1.49e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065049.t1 AT3G54260 61.478 379 132 6 2 376 4 372 1.27e-172 486
MS.gene065049.t1 AT5G58600 40.237 338 185 6 55 378 66 400 8.57e-93 284
MS.gene065049.t1 AT2G31110 38.462 364 198 7 21 374 11 358 7.42e-92 280
MS.gene065049.t1 AT1G29050 41.088 331 175 5 55 378 61 378 1.43e-91 280
MS.gene065049.t1 AT2G34070 39.823 339 181 7 49 378 59 383 1.39e-89 275
MS.gene065049.t1 AT2G30900 40.841 333 174 8 53 374 41 361 1.62e-89 274
MS.gene065049.t1 AT5G01360 38.329 347 191 8 53 378 34 378 5.56e-86 265
MS.gene065049.t1 AT3G62390 38.576 337 187 6 53 374 136 467 2.03e-85 267
MS.gene065049.t1 AT5G01360 38.329 347 191 8 53 378 90 434 2.25e-85 266
MS.gene065049.t1 AT2G30010 40.058 347 179 8 55 378 56 396 2.84e-85 264
MS.gene065049.t1 AT5G06700 37.978 366 202 7 37 378 230 594 3.54e-85 270
MS.gene065049.t1 AT2G30010 40.058 347 179 8 55 378 99 439 6.37e-85 265
MS.gene065049.t1 AT2G42570 38.507 335 180 7 54 378 46 364 1.65e-83 259
MS.gene065049.t1 AT1G78710 38.298 329 182 7 54 374 37 352 1.67e-83 258
MS.gene065049.t1 AT3G12060 37.666 377 206 9 28 378 162 535 3.61e-83 264
MS.gene065049.t1 AT1G73140 38.728 346 187 8 53 378 65 405 3.93e-83 259
MS.gene065049.t1 AT3G55990 37.772 368 199 9 29 374 120 479 6.30e-82 258
MS.gene065049.t1 AT2G40150 38.636 352 180 9 54 374 70 416 2.07e-81 255
MS.gene065049.t1 AT5G20590 38.663 344 190 8 53 378 139 479 5.61e-81 256
MS.gene065049.t1 AT5G49340 37.571 354 199 8 45 378 90 441 3.88e-80 253
MS.gene065049.t1 AT1G60790 36.416 346 199 9 49 374 184 528 2.74e-79 253
MS.gene065049.t1 AT3G14850 37.273 330 184 7 55 374 34 350 5.43e-79 247
MS.gene065049.t1 AT5G01620 37.101 345 195 8 53 378 116 457 9.90e-79 249
MS.gene065049.t1 AT5G01620 37.101 345 195 8 53 378 108 449 1.02e-78 249
MS.gene065049.t1 AT5G01620 37.101 345 195 8 53 378 108 449 1.02e-78 249
MS.gene065049.t1 AT2G40160 37.176 347 195 9 55 378 77 423 3.35e-78 248
MS.gene065049.t1 AT2G40160 36.888 347 196 9 55 378 77 423 4.08e-78 247
MS.gene065049.t1 AT3G11030 37.609 343 195 7 55 378 106 448 1.39e-76 244
MS.gene065049.t1 AT2G38320 32.161 398 243 10 3 378 12 404 8.54e-75 238
MS.gene065049.t1 AT2G40320 34.343 396 235 9 8 378 27 422 1.15e-73 235
MS.gene065049.t1 AT1G48880 35.345 348 180 8 54 378 114 439 1.13e-67 220
MS.gene065049.t1 AT2G31110 38.583 254 153 3 20 272 10 261 1.39e-66 212
MS.gene065049.t1 AT5G19160 34.133 375 209 9 33 376 75 442 1.81e-66 218
MS.gene065049.t1 AT5G58600 41.410 227 130 3 55 279 66 291 3.86e-65 209
MS.gene065049.t1 AT3G62390 41.176 238 129 3 53 279 136 373 4.39e-65 213
MS.gene065049.t1 AT3G06080 35.028 354 195 9 50 376 98 443 9.14e-65 213
MS.gene065049.t1 AT2G38320 33.645 321 193 7 78 378 1 321 9.19e-65 209
MS.gene065049.t1 AT2G30900 42.609 230 127 5 53 279 41 268 1.49e-64 207
MS.gene065049.t1 AT5G06230 34.561 353 197 8 53 378 19 364 1.27e-62 205
MS.gene065049.t1 AT5G06230 34.561 353 197 8 53 378 60 405 2.64e-62 206
MS.gene065049.t1 AT2G34070 38.783 263 139 6 124 378 1 249 2.77e-62 200
MS.gene065049.t1 AT3G11570 31.566 396 232 10 8 378 38 419 5.61e-61 202
MS.gene065049.t1 AT2G40160 39.407 236 132 6 55 279 77 312 1.20e-56 188
MS.gene065049.t1 AT1G78710 31.624 351 173 10 30 374 3 292 8.25e-56 185
MS.gene065049.t1 AT5G20590 38.750 240 134 5 53 279 139 378 7.52e-54 182
MS.gene065049.t1 AT5G19160 37.037 243 140 4 50 279 94 336 1.68e-50 173
MS.gene065049.t1 AT3G14850 35.000 260 147 6 124 374 1 247 9.48e-50 168
MS.gene065049.t1 AT3G06080 35.802 243 143 4 50 279 98 340 1.21e-48 168
MS.gene065049.t1 AT2G31110 39.908 218 106 6 166 374 9 210 2.37e-48 163
MS.gene065049.t1 AT5G06230 34.959 246 141 4 53 279 60 305 1.43e-45 159
MS.gene065049.t1 AT1G70230 30.447 358 190 14 55 378 79 411 1.31e-40 148
MS.gene065049.t1 AT4G25360 29.096 354 211 12 54 374 174 520 2.65e-39 147
MS.gene065049.t1 AT4G25360 29.096 354 211 12 54 374 174 520 2.65e-39 147
MS.gene065049.t1 AT5G51640 28.912 377 223 13 31 374 120 484 1.51e-38 144
MS.gene065049.t1 AT1G01430 26.216 370 237 12 35 374 80 443 3.54e-38 142
MS.gene065049.t1 AT5G64020 27.606 355 219 15 55 376 57 406 4.08e-37 139
MS.gene065049.t1 AT4G01080 28.693 352 211 12 51 374 87 426 4.13e-37 139
MS.gene065049.t1 AT3G28150 28.713 404 215 18 12 376 39 408 1.64e-34 132
MS.gene065049.t1 AT5G20680 28.312 385 217 16 31 378 95 457 5.08e-34 131
MS.gene065049.t1 AT5G20680 28.312 385 217 16 31 378 189 551 9.84e-34 132
MS.gene065049.t1 AT5G20680 28.312 385 217 16 31 378 189 551 9.84e-34 132
MS.gene065049.t1 AT5G20680 28.312 385 217 16 31 378 189 551 9.84e-34 132
MS.gene065049.t1 AT5G20680 28.312 385 217 16 31 378 189 551 9.84e-34 132
MS.gene065049.t1 AT2G37720 28.125 352 217 15 53 378 141 482 1.01e-33 130
MS.gene065049.t1 AT5G20680 28.312 385 217 16 31 378 171 533 1.27e-33 131
MS.gene065049.t1 AT5G15900 30.280 393 205 17 26 378 46 409 1.84e-33 129
MS.gene065049.t1 AT5G15890 27.841 352 214 10 49 374 181 518 2.08e-33 130
MS.gene065049.t1 AT5G15900 30.280 393 205 17 26 378 65 428 2.61e-33 129
MS.gene065049.t1 AT4G11090 28.261 414 244 18 1 374 21 421 7.89e-33 127
MS.gene065049.t1 AT5G64470 26.377 345 222 10 55 375 55 391 2.32e-31 123
MS.gene065049.t1 AT5G64470 26.211 351 221 11 55 375 55 397 5.67e-31 122
MS.gene065049.t1 AT2G14530 26.741 359 214 10 55 378 62 406 6.34e-31 122
MS.gene065049.t1 AT4G23790 27.424 361 209 14 53 374 77 423 1.89e-30 121
MS.gene065049.t1 AT3G02440 28.286 350 209 11 55 374 127 464 2.50e-29 118
MS.gene065049.t1 AT3G02440 28.286 350 209 11 55 374 135 472 2.77e-29 118
MS.gene065049.t1 AT5G64470 26.423 246 162 7 55 283 55 298 3.07e-21 93.6
MS.gene065049.t1 AT5G64470 26.423 246 162 7 55 283 55 298 4.35e-21 93.2

Find 94 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGGTGTGCCTGATATT+TGG 0.189016 1.2:-83025959 MS.gene065049:CDS
GTGATTTCTCAGTTGGAAAA+TGG 0.208372 1.2:-83027356 MS.gene065049:CDS
AGACATCAATTGAGTTCTTC+TGG 0.226253 1.2:-83026720 MS.gene065049:CDS
ATTTCTTCCATGAATCTCTT+TGG 0.226552 1.2:+83027379 None:intergenic
GCTCATTGGTGGACTCATTC+TGG 0.278727 1.2:-83026580 MS.gene065049:CDS
TGATTAAATTGTAGGCAAAA+TGG 0.281278 1.2:-83026224 MS.gene065049:intron
AAAGTGGGTGGATTTGAATT+TGG 0.291594 1.2:-83026357 MS.gene065049:CDS
TTTCTTCCATGAATCTCTTT+GGG 0.298282 1.2:+83027380 None:intergenic
AGCATTTCGTTCCAAATATC+AGG 0.327867 1.2:+83025948 None:intergenic
TTCAGCCACATGCATGTTCC+AGG 0.356693 1.2:-83026164 MS.gene065049:CDS
GTAATATCCTGTAGATATAC+TGG 0.367331 1.2:+83026098 None:intergenic
GGACCAGTAGTAGTGCTAAA+AGG 0.371991 1.2:-83026143 MS.gene065049:CDS
TAATAAGAGAATTCTACATT+TGG 0.381121 1.2:-83026662 MS.gene065049:CDS
TTGAAGAGAATGCAAGGTAT+TGG 0.381566 1.2:-83026633 MS.gene065049:CDS
TATTCCAACCGACAGGAAAA+AGG 0.389139 1.2:-83026865 MS.gene065049:CDS
ATAGCATGGAAAGACATTAA+AGG 0.392314 1.2:+83026828 None:intergenic
TTCTTCCAATGCAAGCCAAC+TGG 0.394214 1.2:+83027428 None:intergenic
TGCAGCCATTGGTGCCTCCC+TGG 0.399442 1.2:-83025975 MS.gene065049:CDS
TTTAATGTCTTTCCATGCTA+TGG 0.404785 1.2:-83026826 MS.gene065049:intron
TTGCATTTGATTCAGCTCAT+TGG 0.406976 1.2:-83026594 MS.gene065049:CDS
TTCTTCAGTTCTACCAATAG+TGG 0.407885 1.2:+83026694 None:intergenic
TGTATTGAGTCCTTTCTCAT+AGG 0.414458 1.2:+83026385 None:intergenic
GTTCTTCTGGGCACCACTAT+TGG 0.416633 1.2:-83026707 MS.gene065049:CDS
CATTTGATTCAGCTCATTGG+TGG 0.419213 1.2:-83026591 MS.gene065049:CDS
GACATCAATTGAGTTCTTCT+GGG 0.424420 1.2:-83026719 MS.gene065049:CDS
AAGAAATGTGATTTCTCAGT+TGG 0.430768 1.2:-83027363 MS.gene065049:CDS
TTTGATGCACTGAAGTATCT+TGG 0.440296 1.2:-83026975 MS.gene065049:CDS
AAATGGAAGTGGAAGCCATT+AGG 0.444002 1.2:-83027234 MS.gene065049:CDS
AGACATTAAAGGACCATCAT+AGG 0.444756 1.2:+83026839 None:intergenic
CATTTCTCATAATCTGAATC+CGG 0.445205 1.2:+83027252 None:intergenic
CTTGGGATGGAACAACCTAA+TGG 0.446475 1.2:+83027219 None:intergenic
AGTCCTCTGATTGCAGCCAT+TGG 0.458504 1.2:-83025986 MS.gene065049:CDS
CGGATTCAGATTATGAGAAA+TGG 0.459664 1.2:-83027251 MS.gene065049:CDS
TTCTATAATGAGAAACCAAT+GGG 0.468469 1.2:-83026916 MS.gene065049:CDS
GTATGGCACCTAGACATAAC+AGG 0.470822 1.2:-83026304 MS.gene065049:intron
TTCCAAATATCAGGCACACC+AGG 0.474779 1.2:+83025957 None:intergenic
TGCAAACCCAAAGAGATTCA+TGG 0.480389 1.2:-83027386 MS.gene065049:CDS
AGTGTCCAGTTGGCTTGCAT+TGG 0.481051 1.2:-83027433 MS.gene065049:CDS
TCTCTTGTATGCTTAGTTCA+AGG 0.493508 1.2:-83026891 MS.gene065049:CDS
CATCACAAACATGAATCAAA+TGG 0.494804 1.2:-83026411 MS.gene065049:CDS
CAAATGGTTGCCTATGAGAA+AGG 0.503172 1.2:-83026395 MS.gene065049:CDS
GAGATTTCCAGTATATCTAC+AGG 0.504762 1.2:-83026105 MS.gene065049:CDS
GCAGTAACTTGTCAAAAGAA+TGG 0.505516 1.2:-83027279 MS.gene065049:CDS
GTTGGAAATGTTACAATCAA+AGG 0.507595 1.2:-83026202 MS.gene065049:CDS
AGCCAACTGGACACTTCCGC+TGG 0.508129 1.2:+83027441 None:intergenic
TAAATTGTAGGCAAAATGGT+TGG 0.508760 1.2:-83026220 MS.gene065049:intron
ATGAGAAAGGACTCAATACA+TGG 0.509591 1.2:-83026382 MS.gene065049:CDS
TTTAGCACTACTACTGGTCC+TGG 0.509768 1.2:+83026146 None:intergenic
TATTGGTAGAACTGAAGAAA+GGG 0.514662 1.2:-83026690 MS.gene065049:CDS
TACCAGGTGGGATTATTACA+TGG 0.524110 1.2:-83026447 MS.gene065049:intron
GCACACCAGGGAGGCACCAA+TGG 0.532334 1.2:+83025970 None:intergenic
GTTACTGCAGTGGCGAGATA+AGG 0.535012 1.2:+83027294 None:intergenic
CAGATTATGAGAAATGGAAG+TGG 0.537613 1.2:-83027245 MS.gene065049:CDS
CTATTGGTAGAACTGAAGAA+AGG 0.537856 1.2:-83026691 MS.gene065049:CDS
ACATCCTTCAGTGTATAGTA+AGG 0.542161 1.2:-83026048 MS.gene065049:CDS
AGCATACAAGAGATTCCCAT+TGG 0.544133 1.2:+83026901 None:intergenic
GACTCAATACATGGGCAAAG+TGG 0.544827 1.2:-83026373 MS.gene065049:CDS
ATGACTGCATTTAGAAGAGA+TGG 0.549263 1.2:-83026071 MS.gene065049:CDS
GAGAGGCAGAAACAAGGTAA+TGG 0.549704 1.2:-83026011 MS.gene065049:CDS
ATTCTATAATGAGAAACCAA+TGG 0.550938 1.2:-83026917 MS.gene065049:CDS
AAGGAGTTATTCCAACCGAC+AGG 0.552744 1.2:-83026872 MS.gene065049:CDS
ATTAATATCCTACCATAGCA+TGG 0.553143 1.2:+83026814 None:intergenic
CATTAGGTTGTTCCATCCCA+AGG 0.558505 1.2:-83027218 MS.gene065049:intron
AGGTAATAAGTGAAGAAGAG+AGG 0.559968 1.2:-83026028 MS.gene065049:CDS
GACAGTTGGAATCATAGAGA+GGG 0.562013 1.2:+83027316 None:intergenic
AGTACGTACCTGTTATGTCT+AGG 0.567486 1.2:+83026296 None:intergenic
GCACCAATGGCTGCAATCAG+AGG 0.571338 1.2:+83025983 None:intergenic
GTTTCATCATCACCTTGGGA+TGG 0.572029 1.2:+83027206 None:intergenic
TTGTCAAAAGAATGGACGGC+CGG 0.574038 1.2:-83027271 MS.gene065049:CDS
ACTCAATACATGGGCAAAGT+GGG 0.582566 1.2:-83026372 MS.gene065049:CDS
GAGAGGAAAGAGAATGATGT+TGG 0.587409 1.2:-83026946 MS.gene065049:CDS
AATGGCTGCAATCAGAGGAC+TGG 0.587580 1.2:+83025988 None:intergenic
GAGAATGCAAGGTATTGGAA+AGG 0.589108 1.2:-83026628 MS.gene065049:CDS
ATTTGATTGAAGAGAATGCA+AGG 0.595081 1.2:-83026639 MS.gene065049:CDS
AAGTATCTTGGTAAAATGAG+AGG 0.596417 1.2:-83026963 MS.gene065049:CDS
AGGTGGGATTATTACATGGA+AGG 0.598017 1.2:-83026443 MS.gene065049:CDS
CAATACATGGGCAAAGTGGG+TGG 0.598037 1.2:-83026369 MS.gene065049:CDS
ATTACCTTACTATACACTGA+AGG 0.598619 1.2:+83026044 None:intergenic
ATCCAGCGGAAGTGTCCAGT+TGG 0.606027 1.2:-83027443 MS.gene065049:CDS
GTTGGAATCATAGAGAGGGT+AGG 0.612157 1.2:+83027320 None:intergenic
TAACTTGTCAAAAGAATGGA+CGG 0.614689 1.2:-83027275 MS.gene065049:CDS
GGAACGAAATGCTGAGTGCA+TGG 0.615044 1.2:-83025938 MS.gene065049:CDS
GAAGAAGAGAGGCAGAAACA+AGG 0.633633 1.2:-83026017 MS.gene065049:CDS
GGACAGTTGGAATCATAGAG+AGG 0.634353 1.2:+83027315 None:intergenic
TCCAAATATCAGGCACACCA+GGG 0.635948 1.2:+83025958 None:intergenic
AGTGGCGAGATAAGGACAGT+TGG 0.638580 1.2:+83027302 None:intergenic
GGAAAGAGAATGATGTTGGT+GGG 0.640717 1.2:-83026942 MS.gene065049:CDS
AGGAAAGAGAATGATGTTGG+TGG 0.644337 1.2:-83026943 MS.gene065049:CDS
AGTAGTAGTGCTAAAAGGAG+TGG 0.649261 1.2:-83026138 MS.gene065049:CDS
TGAGAAAGGACTCAATACAT+GGG 0.677756 1.2:-83026381 MS.gene065049:CDS
CTGGTCCTGGAACATGCATG+TGG 0.678164 1.2:+83026159 None:intergenic
ATTATCACTGATCAATCCAG+CGG 0.715885 1.2:-83027457 MS.gene065049:CDS
AAATATCAGGCACACCAGGG+AGG 0.749580 1.2:+83025961 None:intergenic
AGAAACTGAAGATGTCAACA+TGG 0.749769 1.2:-83027409 MS.gene065049:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAACATTTGATTAAATTGT+AGG - chr1.2:83027219-83027238 MS.gene065049:CDS 20.0%
!! ATAAAAATGACAGTAAATGA+AGG + chr1.2:83025949-83025968 None:intergenic 20.0%
!! TAATAAGAGAATTCTACATT+TGG - chr1.2:83026789-83026808 MS.gene065049:intron 20.0%
!!! CATTTTTAGAAGGAAAATTA+AGG + chr1.2:83026674-83026693 None:intergenic 20.0%
!!! GTAAGTAAATCATTTTTAGA+AGG + chr1.2:83026684-83026703 None:intergenic 20.0%
!!! TAATCATTTTTACATCCTAA+TGG - chr1.2:83026965-83026984 MS.gene065049:CDS 20.0%
! AAGCATGTAAGAAAACAAAA+AGG + chr1.2:83026291-83026310 None:intergenic 25.0%
! AATCAATCTTGATTAATACC+AGG - chr1.2:83026988-83027007 MS.gene065049:intron 25.0%
! ATTCTATAATGAGAAACCAA+TGG - chr1.2:83026534-83026553 MS.gene065049:intron 25.0%
! TGATTAAATTGTAGGCAAAA+TGG - chr1.2:83027227-83027246 MS.gene065049:CDS 25.0%
! TTCTATAATGAGAAACCAAT+GGG - chr1.2:83026535-83026554 MS.gene065049:intron 25.0%
!! TAATCAAGATTGATTCCATT+AGG + chr1.2:83026983-83027002 None:intergenic 25.0%
!!! CTGTCATTTTTATTCTTCTT+AGG - chr1.2:83025956-83025975 MS.gene065049:CDS 25.0%
AAGAAATGTGATTTCTCAGT+TGG - chr1.2:83026088-83026107 MS.gene065049:CDS 30.0%
AAGTATCTTGGTAAAATGAG+AGG - chr1.2:83026488-83026507 MS.gene065049:intron 30.0%
AATCTTGATTAATACCAGGT+GGG - chr1.2:83026992-83027011 MS.gene065049:intron 30.0%
ACATGTTGTTCACTTATTAC+AGG - chr1.2:83026702-83026721 MS.gene065049:CDS 30.0%
AGAAGCTAAATTTATCAGCA+TGG + chr1.2:83026440-83026459 None:intergenic 30.0%
ATAGCATGGAAAGACATTAA+AGG + chr1.2:83026626-83026645 None:intergenic 30.0%
ATTAATATCCTACCATAGCA+TGG + chr1.2:83026640-83026659 None:intergenic 30.0%
ATTACCTTACTATACACTGA+AGG + chr1.2:83027410-83027429 None:intergenic 30.0%
ATTTCTTCCATGAATCTCTT+TGG + chr1.2:83026075-83026094 None:intergenic 30.0%
ATTTGATTGAAGAGAATGCA+AGG - chr1.2:83026812-83026831 MS.gene065049:intron 30.0%
CATCACAAACATGAATCAAA+TGG - chr1.2:83027040-83027059 MS.gene065049:intron 30.0%
CATTTCTCATAATCTGAATC+CGG + chr1.2:83026202-83026221 None:intergenic 30.0%
GTAATATCCTGTAGATATAC+TGG + chr1.2:83027356-83027375 None:intergenic 30.0%
GTTGGAAATGTTACAATCAA+AGG - chr1.2:83027249-83027268 MS.gene065049:CDS 30.0%
TAAATTGTAGGCAAAATGGT+TGG - chr1.2:83027231-83027250 MS.gene065049:CDS 30.0%
TAATGTTTCATCATCACCTT+GGG + chr1.2:83026252-83026271 None:intergenic 30.0%
TATTGGTAGAACTGAAGAAA+GGG - chr1.2:83026761-83026780 MS.gene065049:intron 30.0%
TTAATGTTTCATCATCACCT+TGG + chr1.2:83026253-83026272 None:intergenic 30.0%
TTTAATGTCTTTCCATGCTA+TGG - chr1.2:83026625-83026644 MS.gene065049:CDS 30.0%
TTTCTTCCATGAATCTCTTT+GGG + chr1.2:83026074-83026093 None:intergenic 30.0%
! ATGTGGCTGAAATATTTTAC+AGG + chr1.2:83027278-83027297 None:intergenic 30.0%
! TAACTTGTCAAAAGAATGGA+CGG - chr1.2:83026176-83026195 MS.gene065049:CDS 30.0%
! TGCAACATTTTTGACATGTA+TGG - chr1.2:83026315-83026334 MS.gene065049:CDS 30.0%
ACATCCTTCAGTGTATAGTA+AGG - chr1.2:83027403-83027422 MS.gene065049:CDS 35.0%
AGAAACTGAAGATGTCAACA+TGG - chr1.2:83026042-83026061 MS.gene065049:CDS 35.0%
AGACATCAATTGAGTTCTTC+TGG - chr1.2:83026731-83026750 MS.gene065049:intron 35.0%
AGACATTAAAGGACCATCAT+AGG + chr1.2:83026615-83026634 None:intergenic 35.0%
AGCATTTCGTTCCAAATATC+AGG + chr1.2:83027506-83027525 None:intergenic 35.0%
AGGTAATAAGTGAAGAAGAG+AGG - chr1.2:83027423-83027442 MS.gene065049:CDS 35.0%
ATGACTGCATTTAGAAGAGA+TGG - chr1.2:83027380-83027399 MS.gene065049:CDS 35.0%
ATGAGAAAGGACTCAATACA+TGG - chr1.2:83027069-83027088 MS.gene065049:intron 35.0%
ATTATCACTGATCAATCCAG+CGG - chr1.2:83025994-83026013 MS.gene065049:CDS 35.0%
CAATCTTGATTAATACCAGG+TGG - chr1.2:83026991-83027010 MS.gene065049:intron 35.0%
CAGATTATGAGAAATGGAAG+TGG - chr1.2:83026206-83026225 MS.gene065049:CDS 35.0%
CGGATTCAGATTATGAGAAA+TGG - chr1.2:83026200-83026219 MS.gene065049:CDS 35.0%
CTATTGGTAGAACTGAAGAA+AGG - chr1.2:83026760-83026779 MS.gene065049:intron 35.0%
GACATCAATTGAGTTCTTCT+GGG - chr1.2:83026732-83026751 MS.gene065049:intron 35.0%
GAGATTTCCAGTATATCTAC+AGG - chr1.2:83027346-83027365 MS.gene065049:CDS 35.0%
GTGATTTCTCAGTTGGAAAA+TGG - chr1.2:83026095-83026114 MS.gene065049:CDS 35.0%
TCTCTTGTATGCTTAGTTCA+AGG - chr1.2:83026560-83026579 MS.gene065049:intron 35.0%
TGAGAAAGGACTCAATACAT+GGG - chr1.2:83027070-83027089 MS.gene065049:intron 35.0%
TGTATTGAGTCCTTTCTCAT+AGG + chr1.2:83027069-83027088 None:intergenic 35.0%
TTCTTCAGTTCTACCAATAG+TGG + chr1.2:83026760-83026779 None:intergenic 35.0%
TTGAAGAGAATGCAAGGTAT+TGG - chr1.2:83026818-83026837 MS.gene065049:intron 35.0%
TTGCATTTGATTCAGCTCAT+TGG - chr1.2:83026857-83026876 MS.gene065049:CDS 35.0%
! AAAGTGGGTGGATTTGAATT+TGG - chr1.2:83027094-83027113 MS.gene065049:intron 35.0%
! CATACTTCTAAAAACGACTC+GGG + chr1.2:83027132-83027151 None:intergenic 35.0%
! CCATACTTCTAAAAACGACT+CGG + chr1.2:83027133-83027152 None:intergenic 35.0%
! GCAGTAACTTGTCAAAAGAA+TGG - chr1.2:83026172-83026191 MS.gene065049:CDS 35.0%
! TTTGATGCACTGAAGTATCT+TGG - chr1.2:83026476-83026495 MS.gene065049:intron 35.0%
AAATGGAAGTGGAAGCCATT+AGG - chr1.2:83026217-83026236 MS.gene065049:intron 40.0%
ACTCAATACATGGGCAAAGT+GGG - chr1.2:83027079-83027098 MS.gene065049:intron 40.0%
AGCATACAAGAGATTCCCAT+TGG + chr1.2:83026553-83026572 None:intergenic 40.0%
AGCATGGTTTACGTGTGTTT+GGG + chr1.2:83026424-83026443 None:intergenic 40.0%
AGGAAAAAGGTCACCTATGA+TGG - chr1.2:83026599-83026618 MS.gene065049:CDS 40.0%
AGGAAAGAGAATGATGTTGG+TGG - chr1.2:83026508-83026527 MS.gene065049:intron 40.0%
AGGTGGGATTATTACATGGA+AGG - chr1.2:83027008-83027027 MS.gene065049:intron 40.0%
AGTACGTACCTGTTATGTCT+AGG + chr1.2:83027158-83027177 None:intergenic 40.0%
AGTAGTAGTGCTAAAAGGAG+TGG - chr1.2:83027313-83027332 MS.gene065049:CDS 40.0%
ATGTCTTTCCATGCTATGGT+AGG - chr1.2:83026629-83026648 MS.gene065049:CDS 40.0%
CAAATGGTTGCCTATGAGAA+AGG - chr1.2:83027056-83027075 MS.gene065049:intron 40.0%
CATTTGATTCAGCTCATTGG+TGG - chr1.2:83026860-83026879 MS.gene065049:CDS 40.0%
GACAGTTGGAATCATAGAGA+GGG + chr1.2:83026138-83026157 None:intergenic 40.0%
GAGAGGAAAGAGAATGATGT+TGG - chr1.2:83026505-83026524 MS.gene065049:intron 40.0%
GGAAAGAGAATGATGTTGGT+GGG - chr1.2:83026509-83026528 MS.gene065049:intron 40.0%
TACCAGGTGGGATTATTACA+TGG - chr1.2:83027004-83027023 MS.gene065049:intron 40.0%
TATTCCAACCGACAGGAAAA+AGG - chr1.2:83026586-83026605 MS.gene065049:CDS 40.0%
TGCAAACCCAAAGAGATTCA+TGG - chr1.2:83026065-83026084 MS.gene065049:CDS 40.0%
TTCCATGTAATAATCCCACC+TGG + chr1.2:83027009-83027028 None:intergenic 40.0%
! ACTCCTTTTAGCACTACTAC+TGG + chr1.2:83027314-83027333 None:intergenic 40.0%
! AGCTTCTCGATGACATTTTC+AGG - chr1.2:83026453-83026472 MS.gene065049:intron 40.0%
! ATAGGTGACCTTTTTCCTGT+CGG + chr1.2:83026597-83026616 None:intergenic 40.0%
! CTTTTGACAAGTTACTGCAG+TGG + chr1.2:83026170-83026189 None:intergenic 40.0%
!! GAGAATGCAAGGTATTGGAA+AGG - chr1.2:83026823-83026842 MS.gene065049:intron 40.0%
!!! CCGAGTCGTTTTTAGAAGTA+TGG - chr1.2:83027130-83027149 MS.gene065049:intron 40.0%
AAGGAGTTATTCCAACCGAC+AGG - chr1.2:83026579-83026598 MS.gene065049:CDS 45.0%
CAGCATGGTTTACGTGTGTT+TGG + chr1.2:83026425-83026444 None:intergenic 45.0%
CTTGGGATGGAACAACCTAA+TGG + chr1.2:83026235-83026254 None:intergenic 45.0%
GAAGAAGAGAGGCAGAAACA+AGG - chr1.2:83027434-83027453 MS.gene065049:CDS 45.0%
GACTCAATACATGGGCAAAG+TGG - chr1.2:83027078-83027097 MS.gene065049:intron 45.0%
GAGAGGCAGAAACAAGGTAA+TGG - chr1.2:83027440-83027459 MS.gene065049:CDS 45.0%
GGACAGTTGGAATCATAGAG+AGG + chr1.2:83026139-83026158 None:intergenic 45.0%
GGACCAGTAGTAGTGCTAAA+AGG - chr1.2:83027308-83027327 MS.gene065049:CDS 45.0%
GTATGGCACCTAGACATAAC+AGG - chr1.2:83027147-83027166 MS.gene065049:intron 45.0%
GTTGGAATCATAGAGAGGGT+AGG + chr1.2:83026134-83026153 None:intergenic 45.0%
GTTTCATCATCACCTTGGGA+TGG + chr1.2:83026248-83026267 None:intergenic 45.0%
TCCAAATATCAGGCACACCA+GGG + chr1.2:83027496-83027515 None:intergenic 45.0%
TTCCAAATATCAGGCACACC+AGG + chr1.2:83027497-83027516 None:intergenic 45.0%
TTCTTCCAATGCAAGCCAAC+TGG + chr1.2:83026026-83026045 None:intergenic 45.0%
TTGTCAAAAGAATGGACGGC+CGG - chr1.2:83026180-83026199 MS.gene065049:CDS 45.0%
TTTAGCACTACTACTGGTCC+TGG + chr1.2:83027308-83027327 None:intergenic 45.0%
! CATTAGGTTGTTCCATCCCA+AGG - chr1.2:83026233-83026252 MS.gene065049:intron 45.0%
!! AAAACGACTCGGGTTTTTCG+AGG + chr1.2:83027122-83027141 None:intergenic 45.0%
AAATATCAGGCACACCAGGG+AGG + chr1.2:83027493-83027512 None:intergenic 50.0%
AATGGCTGCAATCAGAGGAC+TGG + chr1.2:83027466-83027485 None:intergenic 50.0%
AGTCCTCTGATTGCAGCCAT+TGG - chr1.2:83027465-83027484 MS.gene065049:CDS 50.0%
AGTGGCGAGATAAGGACAGT+TGG + chr1.2:83026152-83026171 None:intergenic 50.0%
CAATACATGGGCAAAGTGGG+TGG - chr1.2:83027082-83027101 MS.gene065049:intron 50.0%
GGAACGAAATGCTGAGTGCA+TGG - chr1.2:83027513-83027532 MS.gene065049:CDS 50.0%
GTTACTGCAGTGGCGAGATA+AGG + chr1.2:83026160-83026179 None:intergenic 50.0%
GTTCTTCTGGGCACCACTAT+TGG - chr1.2:83026744-83026763 MS.gene065049:intron 50.0%
TCCCTGGTGTGCCTGATATT+TGG - chr1.2:83027492-83027511 MS.gene065049:CDS 50.0%
TTCAGCCACATGCATGTTCC+AGG - chr1.2:83027287-83027306 MS.gene065049:CDS 50.0%
! GTGACCTTTTTCCTGTCGGT+TGG + chr1.2:83026593-83026612 None:intergenic 50.0%
!! AGTGTCCAGTTGGCTTGCAT+TGG - chr1.2:83026018-83026037 MS.gene065049:CDS 50.0%
!! GCTCATTGGTGGACTCATTC+TGG - chr1.2:83026871-83026890 MS.gene065049:CDS 50.0%
CTGGTCCTGGAACATGCATG+TGG + chr1.2:83027295-83027314 None:intergenic 55.0%
GCACCAATGGCTGCAATCAG+AGG + chr1.2:83027471-83027490 None:intergenic 55.0%
!! ATCCAGCGGAAGTGTCCAGT+TGG - chr1.2:83026008-83026027 MS.gene065049:CDS 55.0%
AGCCAACTGGACACTTCCGC+TGG + chr1.2:83026013-83026032 None:intergenic 60.0%
! TGCAGCCATTGGTGCCTCCC+TGG - chr1.2:83027476-83027495 MS.gene065049:CDS 65.0%
!! GCACACCAGGGAGGCACCAA+TGG + chr1.2:83027484-83027503 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 83025932 83027541 83025932 ID=MS.gene065049
chr1.2 mRNA 83025932 83027541 83025932 ID=MS.gene065049.t1;Parent=MS.gene065049
chr1.2 exon 83027219 83027541 83027219 ID=MS.gene065049.t1.exon1;Parent=MS.gene065049.t1
chr1.2 CDS 83027219 83027541 83027219 ID=cds.MS.gene065049.t1;Parent=MS.gene065049.t1
chr1.2 exon 83026827 83026998 83026827 ID=MS.gene065049.t1.exon2;Parent=MS.gene065049.t1
chr1.2 CDS 83026827 83026998 83026827 ID=cds.MS.gene065049.t1;Parent=MS.gene065049.t1
chr1.2 exon 83026568 83026749 83026568 ID=MS.gene065049.t1.exon3;Parent=MS.gene065049.t1
chr1.2 CDS 83026568 83026749 83026568 ID=cds.MS.gene065049.t1;Parent=MS.gene065049.t1
chr1.2 exon 83026305 83026463 83026305 ID=MS.gene065049.t1.exon4;Parent=MS.gene065049.t1
chr1.2 CDS 83026305 83026463 83026305 ID=cds.MS.gene065049.t1;Parent=MS.gene065049.t1
chr1.2 exon 83025932 83026232 83025932 ID=MS.gene065049.t1.exon5;Parent=MS.gene065049.t1
chr1.2 CDS 83025932 83026232 83025932 ID=cds.MS.gene065049.t1;Parent=MS.gene065049.t1
Gene Sequence

>MS.gene065049

ATGGCAAAAACAAACCTTCATTTACTGTCATTTTTATTCTTCTTAGGCTTATTCAACTGCACATTATCACTGATCAATCCAGCGGAAGTGTCCAGTTGGCTTGCATTGGAAGAAACTGAAGATGTCAACATGGTGCAAACCCAAAGAGATTCATGGAAGAAATGTGATTTCTCAGTTGGAAAATGGATATTTGATCAAACCTACCCTCTCTATGATTCCAACTGTCCTTATCTCGCCACTGCAGTAACTTGTCAAAAGAATGGACGGCCGGATTCAGATTATGAGAAATGGAAGTGGAAGCCATTAGGTTGTTCCATCCCAAGGTGATGATGAAACATTAATTGCTCACATTGCTACCTTTTTGTTTTCTTACATGCTTGCATTGCAACATTTTTGACATGTATGGATTTTCTCTACAATTGATTTTTAGTCGACTTTTATGTAAATGTATTCAAACATAAATCACTTTACATTTAACTCACTTTTAGTCCCAAACACACGTAAACCATGCTGATAAATTTAGCTTCTCGATGACATTTTCAGGTTTGATGCACTGAAGTATCTTGGTAAAATGAGAGGAAAGAGAATGATGTTGGTGGGTGATTCTATAATGAGAAACCAATGGGAATCTCTTGTATGCTTAGTTCAAGGAGTTATTCCAACCGACAGGAAAAAGGTCACCTATGATGGTCCTTTAATGTCTTTCCATGCTATGGTAGGATATTAATCAATCTTATATCCTTAATTTTCCTTCTAAAAATGATTTACTTACATGTTGTTCACTTATTACAGGATTTTGAGACATCAATTGAGTTCTTCTGGGCACCACTATTGGTAGAACTGAAGAAAGGGTCTAATAATAAGAGAATTCTACATTTGGATTTGATTGAAGAGAATGCAAGGTATTGGAAAGGAGTTGATATTCTTGCATTTGATTCAGCTCATTGGTGGACTCATTCTGGTCAATCTAGCTCGTAAGAAACATTTCTCATTTTTTAGTTTTGCACTTAATTGAAAGTGTTACATTTGAAGATAATCATTTTTACATCCTAATGGAATCAATCTTGATTAATACCAGGTGGGATTATTACATGGAAGGAAATAGTATCATCACAAACATGAATCAAATGGTTGCCTATGAGAAAGGACTCAATACATGGGCAAAGTGGGTGGATTTGAATTTGGATCCTCGAAAAACCCGAGTCGTTTTTAGAAGTATGGCACCTAGACATAACAGGTACGTACTATCAATATTTTATTTTTGCTTTAGTCTCAATAACATATTTAAGAACATTTGATTAAATTGTAGGCAAAATGGTTGGAAATGTTACAATCAAAGGCAACCTGTAAAATATTTCAGCCACATGCATGTTCCAGGACCAGTAGTAGTGCTAAAAGGAGTGGTGAAGAGAATGAGATTTCCAGTATATCTACAGGATATTACAACAATGACTGCATTTAGAAGAGATGGACATCCTTCAGTGTATAGTAAGGTAATAAGTGAAGAAGAGAGGCAGAAACAAGGTAATGGCCAGTCCTCTGATTGCAGCCATTGGTGCCTCCCTGGTGTGCCTGATATTTGGAACGAAATGCTGAGTGCATGGCTTTAA
Protein sequence

>MS.gene065049.t1

MAKTNLHLLSFLFFLGLFNCTLSLINPAEVSSWLALEETEDVNMVQTQRDSWKKCDFSVGKWIFDQTYPLYDSNCPYLATAVTCQKNGRPDSDYEKWKWKPLGCSIPRFDALKYLGKMRGKRMMLVGDSIMRNQWESLVCLVQGVIPTDRKKVTYDGPLMSFHAMDFETSIEFFWAPLLVELKKGSNNKRILHLDLIEENARYWKGVDILAFDSAHWWTHSGQSSSWDYYMEGNSIITNMNQMVAYEKGLNTWAKWVDLNLDPRKTRVVFRSMAPRHNRQNGWKCYNQRQPVKYFSHMHVPGPVVVLKGVVKRMRFPVYLQDITTMTAFRRDGHPSVYSKVISEEERQKQGNGQSSDCSHWCLPGVPDIWNEMLSAWL