AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065200.t1 MTR_8g068890 98.418 316 4 1 1 316 1 315 0.0 632
MS.gene065200.t1 MTR_5g032420 81.366 322 54 4 1 316 1 322 1.14e-178 496
MS.gene065200.t1 MTR_3g100570 74.763 317 75 3 1 316 1 313 8.42e-173 481
MS.gene065200.t1 MTR_8g086070 38.941 321 150 6 3 316 18 299 1.71e-66 211
MS.gene065200.t1 MTR_7g066690 37.299 311 166 6 6 314 17 300 1.56e-61 198
MS.gene065200.t1 MTR_4g018750 36.422 313 170 6 6 316 15 300 7.80e-61 196
MS.gene065200.t1 MTR_1g090330 33.333 303 176 4 11 312 19 296 8.89e-54 178
MS.gene065200.t1 MTR_3g462140 33.013 312 183 3 4 314 13 299 2.97e-50 169
MS.gene065200.t1 MTR_4g018750 34.783 276 151 6 6 279 15 263 1.86e-44 153
MS.gene065200.t1 MTR_4g018750 28.324 173 119 3 144 311 31 203 2.29e-15 75.5
MS.gene065200.t1 MTR_8g086070 38.961 231 98 5 3 229 18 209 5.49e-43 147
MS.gene065200.t1 MTR_1g088995 31.270 307 178 5 6 312 33 306 1.00e-40 144
MS.gene065200.t1 MTR_1g088995 30.045 223 124 4 6 228 33 223 1.48e-20 89.0
MS.gene065200.t1 MTR_4g057850 25.723 311 189 9 7 309 23 299 1.88e-19 87.4
MS.gene065200.t1 MTR_4g057850 28.426 197 120 4 115 303 9 192 8.44e-13 68.2
MS.gene065200.t1 MTR_4g012850 22.684 313 201 6 6 313 16 292 2.22e-16 78.6
MS.gene065200.t1 MTR_4g012850 28.436 211 116 6 9 217 117 294 5.79e-16 77.4
MS.gene065200.t1 MTR_1g087840 24.746 295 193 7 22 313 35 303 3.20e-16 78.2
MS.gene065200.t1 MTR_8g027975 23.669 338 197 10 4 313 49 353 4.50e-16 78.2
MS.gene065200.t1 MTR_1g097890 26.990 289 185 8 33 313 34 304 7.62e-16 77.0
MS.gene065200.t1 MTR_5g009500 21.856 334 206 8 4 313 36 338 1.10e-15 77.0
MS.gene065200.t1 MTR_2g102010 27.517 298 172 12 4 294 15 275 8.14e-14 71.2
MS.gene065200.t1 MTR_2g102010 25.397 189 113 4 22 208 126 288 6.13e-11 62.8
MS.gene065200.t1 MTR_5g055410 27.063 303 176 12 22 313 37 305 9.20e-14 71.6
MS.gene065200.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 1.43e-13 69.7
MS.gene065200.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 1.88e-13 69.7
MS.gene065200.t1 MTR_3g104500 23.016 252 171 7 74 314 83 322 2.00e-13 70.1
MS.gene065200.t1 MTR_5g073040 25.159 314 191 7 11 316 40 317 2.38e-13 70.1
MS.gene065200.t1 MTR_4g119770 23.529 340 204 13 5 316 17 328 2.75e-13 69.7
MS.gene065200.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 2.78e-13 69.7
MS.gene065200.t1 MTR_4g088545 23.006 326 204 9 2 313 8 300 8.05e-12 65.5
MS.gene065200.t1 MTR_4g114320 24.490 245 168 6 76 315 164 396 9.06e-13 68.6
MS.gene065200.t1 MTR_5g030530 25.723 311 197 8 9 313 14 296 9.45e-13 67.8
MS.gene065200.t1 MTR_6g091800 25.943 212 133 6 115 311 5 207 1.32e-12 67.8
MS.gene065200.t1 MTR_7g109135 26.214 309 197 9 9 313 25 306 1.69e-12 67.4
MS.gene065200.t1 MTR_8g096050 24.923 325 178 11 6 313 227 502 2.82e-12 67.4
MS.gene065200.t1 MTR_8g076760 22.801 307 195 9 21 314 29 306 3.01e-12 66.6
MS.gene065200.t1 MTR_8g066975 26.498 317 187 12 9 313 14 296 3.97e-12 66.2
MS.gene065200.t1 MTR_8g092080 22.642 318 180 9 4 315 50 307 6.09e-12 65.9
MS.gene065200.t1 MTR_7g068340 30.337 178 105 6 144 315 103 267 8.09e-12 65.9
MS.gene065200.t1 MTR_6g034515 24.262 305 168 9 21 310 82 338 3.15e-11 63.9
MS.gene065200.t1 MTR_1g012030 23.457 243 170 6 76 313 179 410 4.82e-11 63.5
MS.gene065200.t1 MTR_7g117030 24.771 327 211 10 12 315 23 337 6.10e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065200.t1 AT4G24570 74.214 318 75 2 1 316 1 313 4.94e-169 472
MS.gene065200.t1 AT2G22500 75.316 316 73 1 1 316 1 311 1.27e-165 463
MS.gene065200.t1 AT5G09470 59.587 339 108 5 1 315 1 334 1.15e-124 360
MS.gene065200.t1 AT5G19760 39.498 319 147 5 3 314 15 294 1.57e-69 218
MS.gene065200.t1 AT5G58970 38.535 314 160 6 6 313 12 298 3.56e-65 207
MS.gene065200.t1 AT3G54110 37.898 314 168 6 4 316 13 300 1.39e-63 203
MS.gene065200.t1 AT1G14140 33.660 306 172 4 11 312 21 299 1.30e-51 172
MS.gene065200.t1 AT1G14140 26.601 203 123 2 9 211 121 297 3.88e-13 69.3
MS.gene065200.t1 AT4G03115 32.476 311 172 5 3 312 35 308 1.58e-46 159
MS.gene065200.t1 AT4G03115 32.476 311 172 5 3 312 67 340 3.22e-46 160
MS.gene065200.t1 AT5G58970 37.751 249 129 5 6 248 12 240 4.66e-44 152
MS.gene065200.t1 AT5G58970 26.590 173 122 3 144 311 32 204 3.05e-18 83.2
MS.gene065200.t1 AT4G03115 34.137 249 151 4 65 312 9 245 4.07e-42 146
MS.gene065200.t1 AT4G03115 34.583 240 144 4 74 312 10 237 9.79e-41 142
MS.gene065200.t1 AT4G03115 30.396 227 121 4 3 228 35 225 2.18e-24 99.4
MS.gene065200.t1 AT4G03115 30.396 227 121 4 3 228 35 225 3.00e-24 99.0
MS.gene065200.t1 AT5G01340 26.433 314 194 8 7 314 18 300 3.81e-22 94.7
MS.gene065200.t1 AT5G01340 27.869 183 125 3 122 303 11 187 2.13e-11 64.3
MS.gene065200.t1 AT2G33820 25.152 330 190 11 5 313 13 306 1.70e-16 79.0
MS.gene065200.t1 AT1G25380 26.821 302 183 9 22 315 37 308 2.48e-16 79.0
MS.gene065200.t1 AT1G25380 26.821 302 183 9 22 315 37 308 2.48e-16 79.0
MS.gene065200.t1 AT5G56450 25.100 251 165 7 75 314 88 326 7.84e-16 77.4
MS.gene065200.t1 AT4G01100 25.074 339 205 13 4 313 40 358 8.30e-16 77.8
MS.gene065200.t1 AT4G01100 25.000 340 190 12 4 313 40 344 1.48e-15 76.6
MS.gene065200.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.84e-15 75.9
MS.gene065200.t1 AT5G46800 22.535 213 145 3 110 313 1 202 6.19e-11 62.8
MS.gene065200.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.84e-15 75.9
MS.gene065200.t1 AT5G46800 22.535 213 145 3 110 313 1 202 6.19e-11 62.8
MS.gene065200.t1 AT5G46800 25.705 319 190 10 9 313 14 299 1.84e-15 75.9
MS.gene065200.t1 AT5G46800 22.535 213 145 3 110 313 1 202 6.19e-11 62.8
MS.gene065200.t1 AT3G53940 24.159 327 196 10 3 313 70 360 2.40e-15 76.3
MS.gene065200.t1 AT1G34065 23.567 314 182 6 4 315 73 330 3.01e-15 75.9
MS.gene065200.t1 AT1G34065 24.204 314 180 7 4 315 80 337 3.38e-15 75.5
MS.gene065200.t1 AT2G33820 24.556 338 190 12 5 313 13 314 5.60e-14 72.0
MS.gene065200.t1 AT4G01100 26.792 265 157 9 78 313 38 294 6.45e-14 71.6
MS.gene065200.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.94e-13 69.7
MS.gene065200.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.94e-13 69.7
MS.gene065200.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.94e-13 69.7
MS.gene065200.t1 AT3G53940 26.425 193 125 4 132 312 73 260 7.71e-13 68.2
MS.gene065200.t1 AT2G37890 22.118 321 208 9 4 313 43 332 1.20e-12 68.2
MS.gene065200.t1 AT2G47490 27.213 305 172 12 22 313 33 300 1.34e-12 67.8
MS.gene065200.t1 AT2G33820 23.970 267 145 9 5 243 13 249 1.75e-12 67.0
MS.gene065200.t1 AT5G66380 28.718 195 121 8 130 316 12 196 2.17e-12 67.0
MS.gene065200.t1 AT2G35800 24.841 314 185 10 3 314 542 806 2.53e-12 68.2
MS.gene065200.t1 AT4G26180 24.537 216 143 7 109 311 1 209 3.78e-12 66.2
MS.gene065200.t1 AT3G55640 25.000 196 130 5 132 315 38 228 4.21e-12 65.5
MS.gene065200.t1 AT3G55640 25.000 196 130 5 132 315 38 228 4.21e-12 65.5
MS.gene065200.t1 AT1G07030 25.767 326 185 10 6 316 36 319 4.41e-12 66.2
MS.gene065200.t1 AT3G51870 28.977 176 116 4 141 315 104 271 6.16e-12 66.2
MS.gene065200.t1 AT3G51870 24.324 296 182 10 20 313 105 360 1.08e-11 65.5
MS.gene065200.t1 AT4G26180 24.537 216 143 7 109 311 1 209 7.16e-12 65.9
MS.gene065200.t1 AT3G55640 24.747 198 136 4 126 315 36 228 7.27e-12 65.9
MS.gene065200.t1 AT3G55640 24.747 198 136 4 126 315 36 228 7.27e-12 65.9
MS.gene065200.t1 AT4G27940 24.047 341 218 10 12 314 71 408 1.94e-11 64.7
MS.gene065200.t1 AT5G17400 23.793 290 185 7 6 287 14 275 4.40e-11 63.2
MS.gene065200.t1 AT5G01500 29.121 182 110 8 140 315 131 299 4.73e-11 63.5
MS.gene065200.t1 AT5G51050 27.513 189 126 5 132 316 223 404 9.40e-11 62.8

Find 128 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATCCTATGATGAGTTTA+AGG 0.221358 8.1:-36212769 MS.gene065200:CDS
GGGTCCTATGGCTCTTTATA+AGG 0.268391 8.1:-36212544 MS.gene065200:CDS
CGGCCCAACACGGGGCGGTT+GGG 0.300952 8.1:+36213196 None:intergenic
GGGCTGCTCGCCGGTGGGAT+TGG 0.301666 8.1:-36212990 MS.gene065200:CDS
GTGGGATTGGCGCGGCTGTC+GGG 0.356937 8.1:-36212977 MS.gene065200:CDS
CGGACTTATAGTTTCTTCTC+TGG 0.360466 8.1:+36212898 None:intergenic
TGCTAATGCTGGTCGGATAT+TGG 0.367208 8.1:+36213259 None:intergenic
CGGCCCAACCGCCCCGTGTT+GGG 0.379107 8.1:-36213199 MS.gene065200:CDS
ACAGGTTCGTAAGTTGCTTA+AGG 0.385790 8.1:-36212460 MS.gene065200:CDS
GGTGGGATTGGCGCGGCTGT+CGG 0.392305 8.1:-36212978 MS.gene065200:CDS
TGGCGCGGCTGTCGGGAATC+CGG 0.394055 8.1:-36212970 MS.gene065200:CDS
GGTCCTATGGCTCTTTATAA+GGG 0.398934 8.1:-36212543 MS.gene065200:CDS
TCGGCCCAACACGGGGCGGT+TGG 0.400715 8.1:+36213195 None:intergenic
ACGGCCCAACCGCCCCGTGT+TGG 0.410440 8.1:-36213200 MS.gene065200:CDS
CATGGCGCGGTTCACTGTTA+AGG 0.413689 8.1:+36212812 None:intergenic
ACAAACAACACAACGGTAAA+CGG 0.423325 8.1:+36212492 None:intergenic
GCTAGTTACGGCGTCACAAC+TGG 0.426493 8.1:-36212790 MS.gene065200:CDS
GTTGTTTGTTACATTGGAAC+AGG 0.435604 8.1:-36212478 MS.gene065200:CDS
GGCATAGCTTCCATCATTGC+AGG 0.435640 8.1:-36213353 MS.gene065200:CDS
GGTTGGATGAAGGATGGGCT+TGG 0.436837 8.1:-36212729 MS.gene065200:CDS
AAGTTGCTTAAGGATTTCTA+AGG 0.437271 8.1:-36212450 None:intergenic
GGTCCCTGCCTTGTAATTGT+AGG 0.442748 8.1:+36212513 None:intergenic
ATTGGAGCGGGGCTGCTCGC+CGG 0.444367 8.1:-36212999 MS.gene065200:CDS
TTGCCGTTGACAAGTAAGAT+TGG 0.444465 8.1:-36213017 MS.gene065200:CDS
CGTTGTGTTGTTTGTTACAT+TGG 0.449852 8.1:-36212484 MS.gene065200:CDS
TCCAACCCGGTTCGGTTCAT+CGG 0.451801 8.1:-36213235 MS.gene065200:CDS
CTCTATTCCACCACCCGTAT+GGG 0.452624 8.1:-36213089 MS.gene065200:CDS
GGCGGTTGGGCCGTCACAGC+CGG 0.453495 8.1:+36213209 None:intergenic
CAATCAATGGCGCCGGAGTA+AGG 0.454657 8.1:+36212591 None:intergenic
TCCCTCGTCTTTCGCCATCC+TGG 0.455818 8.1:+36212860 None:intergenic
GGAGCGGGGCTGCTCGCCGG+TGG 0.465110 8.1:-36212996 MS.gene065200:CDS
TGGTGTTAAGCTAGTCCAAC+AGG 0.465340 8.1:-36213165 MS.gene065200:CDS
GTTGGATGAAGGATGGGCTT+GGG 0.467370 8.1:-36212728 MS.gene065200:CDS
AACGGCGATCGGCCCAACAC+GGG 0.470119 8.1:+36213187 None:intergenic
CATTCCTACAATTACAAGGC+AGG 0.471330 8.1:-36212517 MS.gene065200:CDS
GGCGGCGGTTGCGTCGAATC+CGG 0.471512 8.1:-36212670 MS.gene065200:CDS
CGGTGACCGATGAACCGAAC+CGG 0.473065 8.1:+36213229 None:intergenic
ATTAGCATTCCAACCCGGTT+CGG 0.477760 8.1:-36213243 MS.gene065200:CDS
CGGTGGATGTGATTAAGACT+AGG 0.482921 8.1:-36212650 MS.gene065200:CDS
CAACGGCGATCGGCCCAACA+CGG 0.483085 8.1:+36213186 None:intergenic
TTGAAGACTGTTCGTGCTGA+GGG 0.486561 8.1:-36212564 MS.gene065200:CDS
GGACTTATAGTTTCTTCTCT+GGG 0.489330 8.1:+36212899 None:intergenic
GGAGTAAGGTGGAGGCGATC+CGG 0.492548 8.1:+36212605 None:intergenic
TGTTCGTGCTGAGGGTCCTA+TGG 0.492843 8.1:-36212556 MS.gene065200:CDS
TGTTGAACACCCTGCAATGA+TGG 0.493211 8.1:+36213343 None:intergenic
AAGGTTCGAATGCAGCTTCA+AGG 0.496054 8.1:-36213302 MS.gene065200:CDS
TTGACAAGTAAGATTGGAGC+GGG 0.502593 8.1:-36213011 MS.gene065200:CDS
GCTGGTCGGATATTGGTTGT+AGG 0.505194 8.1:+36213266 None:intergenic
TCTCTATTCCACCACCCGTA+TGG 0.506394 8.1:-36213090 MS.gene065200:CDS
ATGAATATGAAGGTGGAGGC+CGG 0.509995 8.1:-36212624 MS.gene065200:CDS
AGTCTTCAAAGCACAATCAA+TGG 0.510639 8.1:+36212578 None:intergenic
CGTGTTGGGCCGATCGCCGT+TGG 0.512777 8.1:-36213185 MS.gene065200:CDS
GTGTCTCTGCCACCGTTCTC+CGG 0.514808 8.1:-36213118 MS.gene065200:CDS
AACGGCAAAGTACCGGAGAT+AGG 0.516962 8.1:+36213032 None:intergenic
AGCTTAACACCAACGGCGAT+CGG 0.517276 8.1:+36213176 None:intergenic
AAAATGGTTGGATGAAGGAT+GGG 0.517458 8.1:-36212734 MS.gene065200:CDS
TGTCGGGAATCCGGCTGATG+TGG 0.521033 8.1:-36212961 MS.gene065200:CDS
CGGCTGATGTGGCAATGGTT+AGG 0.521248 8.1:-36212950 MS.gene065200:CDS
GAACCGCGCCATGCTAGTTA+CGG 0.521850 8.1:-36212802 MS.gene065200:CDS
TCATCGGTCACCGGCTGTGA+CGG 0.522088 8.1:-36213219 MS.gene065200:CDS
ATAGAGTCCCATACGGGTGG+TGG 0.523945 8.1:+36213082 None:intergenic
GAGCGGGGCTGCTCGCCGGT+GGG 0.525418 8.1:-36212995 MS.gene065200:CDS
AATAATCGCAATGGGTGTGA+AGG 0.526131 8.1:-36213390 None:intergenic
CTAACCATTGCCACATCAGC+CGG 0.528201 8.1:+36212951 None:intergenic
TAGTTTCTTCTCTGGGCCGA+TGG 0.537125 8.1:+36212906 None:intergenic
AAATGGTCTGATCCTATCTC+CGG 0.538460 8.1:-36213044 MS.gene065200:CDS
ACCGATGAACCGAACCGGGT+TGG 0.539068 8.1:+36213234 None:intergenic
GACAGCCGCGCCAATCCCAC+CGG 0.544361 8.1:+36212980 None:intergenic
TTTGAAGACTGTTCGTGCTG+AGG 0.545035 8.1:-36212565 MS.gene065200:CDS
ACGAGGGAGTTACTAGCCTA+TGG 0.549664 8.1:-36212845 MS.gene065200:CDS
TGTGGCAATGGTTAGGATGC+AGG 0.550254 8.1:-36212943 MS.gene065200:CDS
ACTTGTCAACGGCAAAGTAC+CGG 0.558706 8.1:+36213025 None:intergenic
TTAAGGATGAACCGCGCCAT+AGG 0.560898 8.1:+36212829 None:intergenic
GTTTCATTCCTACAATTACA+AGG 0.563854 8.1:-36212521 MS.gene065200:CDS
GTGCTGCTACACCTTCCTGT+TGG 0.566711 8.1:+36213150 None:intergenic
CAAAGCACAATCAATGGCGC+CGG 0.568351 8.1:+36212584 None:intergenic
CGGTTCGGTTCATCGGTCAC+CGG 0.569766 8.1:-36213228 MS.gene065200:CDS
ATAATCGCAATGGGTGTGAA+GGG 0.570571 8.1:-36213389 None:intergenic
AAACCCTTATAAAGAGCCAT+AGG 0.570705 8.1:+36212540 None:intergenic
GAGAACGGTGGCAGAGACAC+CGG 0.571761 8.1:+36213121 None:intergenic
ATGGCGCCGGAGTAAGGTGG+AGG 0.572524 8.1:+36212597 None:intergenic
CCGGGTTGGAATGCTAATGC+TGG 0.573037 8.1:+36213248 None:intergenic
GGTGTGAAGGGTTTCGTCGA+AGG 0.574034 8.1:-36213377 MS.gene065200:CDS
CCGTCGTCGATGCTATCACC+AGG 0.578930 8.1:-36212878 MS.gene065200:CDS
GGCCGACGGAAGACTTCCAT+CGG 0.579243 8.1:-36212922 MS.gene065200:CDS
GGTGACCGATGAACCGAACC+GGG 0.580897 8.1:+36213230 None:intergenic
GGTGGATGTGATTAAGACTA+GGG 0.581075 8.1:-36212649 MS.gene065200:CDS
CACCAGGATGGCGAAAGACG+AGG 0.583614 8.1:-36212862 MS.gene065200:CDS
TCATATCATAGAGTCCCATA+CGG 0.584545 8.1:+36213075 None:intergenic
AACCGCCGCCACAAAACCCG+CGG 0.588953 8.1:+36212683 None:intergenic
GGTGATGAATATGAAGGTGG+AGG 0.589447 8.1:-36212628 MS.gene065200:CDS
ACGGCGATCGGCCCAACACG+GGG 0.592000 8.1:+36213188 None:intergenic
ATTCCTACAATTACAAGGCA+GGG 0.592283 8.1:-36212516 MS.gene065200:CDS
TTGTGACGCCGTAACTAGCA+TGG 0.595690 8.1:+36212794 None:intergenic
CATATCATAGAGTCCCATAC+GGG 0.597212 8.1:+36213076 None:intergenic
GACTAGGGTGATGAATATGA+AGG 0.600049 8.1:-36212634 MS.gene065200:CDS
ACCAGGATGGCGAAAGACGA+GGG 0.600800 8.1:-36212861 MS.gene065200:CDS
CTAGTCTTAATCACATCCAC+CGG 0.603477 8.1:+36212651 None:intergenic
GAATCCGGCTGATGTGGCAA+TGG 0.605065 8.1:-36212955 MS.gene065200:CDS
ACACCCTCTTGATCTCATCA+AGG 0.606884 8.1:-36213321 MS.gene065200:CDS
GTGAAGGGTTTCGTCGAAGG+AGG 0.607310 8.1:-36213374 MS.gene065200:CDS
GCTCCAATCTTACTTGTCAA+CGG 0.608573 8.1:+36213014 None:intergenic
CGAACCTTGATGAGATCAAG+AGG 0.611493 8.1:+36213317 None:intergenic
GGCCGATGGAAGTCTTCCGT+CGG 0.612610 8.1:+36212920 None:intergenic
GGCGGTTGCGTCGAATCCGG+TGG 0.613152 8.1:-36212667 MS.gene065200:CDS
ATAGAGACACTGCCGGAGAA+CGG 0.615334 8.1:+36213106 None:intergenic
ATGGTTAGGATGCAGGCCGA+CGG 0.622979 8.1:-36212936 MS.gene065200:CDS
TAGGGTGATGAATATGAAGG+TGG 0.625045 8.1:-36212631 MS.gene065200:CDS
CCAGCATTAGCATTCCAACC+CGG 0.630776 8.1:-36213248 MS.gene065200:CDS
GCTCGCCGGTGGGATTGGCG+CGG 0.632649 8.1:-36212985 MS.gene065200:CDS
GGATCGCCTCCACCTTACTC+CGG 0.634705 8.1:-36212603 MS.gene065200:CDS
TGGTGGAATAGAGACACTGC+CGG 0.636597 8.1:+36213099 None:intergenic
TTGGACTAGCTTAACACCAA+CGG 0.639677 8.1:+36213169 None:intergenic
GTTAAGCTAGTCCAACAGGA+AGG 0.649653 8.1:-36213161 MS.gene065200:CDS
GCATAGCTTCCATCATTGCA+GGG 0.651032 8.1:-36213352 MS.gene065200:CDS
GTTGGAATGCTAATGCTGGT+CGG 0.653431 8.1:+36213252 None:intergenic
GTTGACAAGTAAGATTGGAG+CGG 0.654040 8.1:-36213012 MS.gene065200:CDS
CGTCGATGCTATCACCAGGA+TGG 0.654939 8.1:-36212874 MS.gene065200:CDS
TCAATGGCGCCGGAGTAAGG+TGG 0.657611 8.1:+36212594 None:intergenic
ATCATAGAGTCCCATACGGG+TGG 0.658486 8.1:+36213079 None:intergenic
GCGATCGGCCCAACACGGGG+CGG 0.664344 8.1:+36213191 None:intergenic
ACGCCGTAACTAGCATGGCG+CGG 0.673464 8.1:+36212799 None:intergenic
GAGACACTGCCGGAGAACGG+TGG 0.689533 8.1:+36213109 None:intergenic
CAATGTAACAAACAACACAA+CGG 0.699064 8.1:+36212485 None:intergenic
GAACCTTGATGAGATCAAGA+GGG 0.713345 8.1:+36213318 None:intergenic
GGAGTTACTAGCCTATGGCG+CGG 0.742480 8.1:-36212840 MS.gene065200:CDS
CCTGGTGATAGCATCGACGA+CGG 0.744553 8.1:+36212878 None:intergenic
TGACAAGTAAGATTGGAGCG+GGG 0.755189 8.1:-36213010 MS.gene065200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGATTTTAAAAAATGGT+TGG - chr8.1:36213086-36213105 MS.gene065200:CDS 15.0%
!!! AAGGAAAAGATTTTAAAAAA+TGG - chr8.1:36213082-36213101 MS.gene065200:CDS 15.0%
!! ATGATATGATGAAGAAAAAA+TGG - chr8.1:36212771-36212790 MS.gene065200:CDS 20.0%
! TTTAAAAAATGGTTGGATGA+AGG - chr8.1:36213093-36213112 MS.gene065200:CDS 25.0%
CAATGTAACAAACAACACAA+CGG + chr8.1:36213350-36213369 None:intergenic 30.0%
GTTTCATTCCTACAATTACA+AGG - chr8.1:36213311-36213330 MS.gene065200:CDS 30.0%
! CTTTTCCTTAAACTCATCAT+AGG + chr8.1:36213071-36213090 None:intergenic 30.0%
AAAAATGGTTGGATGAAGGA+TGG - chr8.1:36213097-36213116 MS.gene065200:CDS 35.0%
AAAATGGTTGGATGAAGGAT+GGG - chr8.1:36213098-36213117 MS.gene065200:CDS 35.0%
AAACCCTTATAAAGAGCCAT+AGG + chr8.1:36213295-36213314 None:intergenic 35.0%
ACAAACAACACAACGGTAAA+CGG + chr8.1:36213343-36213362 None:intergenic 35.0%
AGTCTTCAAAGCACAATCAA+TGG + chr8.1:36213257-36213276 None:intergenic 35.0%
ATTCCTACAATTACAAGGCA+GGG - chr8.1:36213316-36213335 MS.gene065200:CDS 35.0%
GGACTTATAGTTTCTTCTCT+GGG + chr8.1:36212936-36212955 None:intergenic 35.0%
TCATATCATAGAGTCCCATA+CGG + chr8.1:36212760-36212779 None:intergenic 35.0%
! GTTGTTTGTTACATTGGAAC+AGG - chr8.1:36213354-36213373 MS.gene065200:CDS 35.0%
!! CGTTGTGTTGTTTGTTACAT+TGG - chr8.1:36213348-36213367 MS.gene065200:CDS 35.0%
AAATGGTCTGATCCTATCTC+CGG - chr8.1:36212788-36212807 MS.gene065200:CDS 40.0%
ACAGGTTCGTAAGTTGCTTA+AGG - chr8.1:36213372-36213391 MS.gene065200:CDS 40.0%
CATATCATAGAGTCCCATAC+GGG + chr8.1:36212759-36212778 None:intergenic 40.0%
CATTCCTACAATTACAAGGC+AGG - chr8.1:36213315-36213334 MS.gene065200:CDS 40.0%
CGGACTTATAGTTTCTTCTC+TGG + chr8.1:36212937-36212956 None:intergenic 40.0%
GAACCTTGATGAGATCAAGA+GGG + chr8.1:36212517-36212536 None:intergenic 40.0%
GACTAGGGTGATGAATATGA+AGG - chr8.1:36213198-36213217 MS.gene065200:CDS 40.0%
GCTCCAATCTTACTTGTCAA+CGG + chr8.1:36212821-36212840 None:intergenic 40.0%
GGAAAAACTAGCTGTCACAT+GGG + chr8.1:36213131-36213150 None:intergenic 40.0%
GGCATCCTATGATGAGTTTA+AGG - chr8.1:36213063-36213082 MS.gene065200:CDS 40.0%
GGTCCTATGGCTCTTTATAA+GGG - chr8.1:36213289-36213308 MS.gene065200:CDS 40.0%
GGTGGATGTGATTAAGACTA+GGG - chr8.1:36213183-36213202 MS.gene065200:CDS 40.0%
GTTGACAAGTAAGATTGGAG+CGG - chr8.1:36212820-36212839 MS.gene065200:CDS 40.0%
TAGGGTGATGAATATGAAGG+TGG - chr8.1:36213201-36213220 MS.gene065200:CDS 40.0%
TTGACAAGTAAGATTGGAGC+GGG - chr8.1:36212821-36212840 MS.gene065200:CDS 40.0%
TTGCCGTTGACAAGTAAGAT+TGG - chr8.1:36212815-36212834 MS.gene065200:CDS 40.0%
!! CTAGTCTTAATCACATCCAC+CGG + chr8.1:36213184-36213203 None:intergenic 40.0%
!! TTGGACTAGCTTAACACCAA+CGG + chr8.1:36212666-36212685 None:intergenic 40.0%
AAGGTTCGAATGCAGCTTCA+AGG - chr8.1:36212530-36212549 MS.gene065200:CDS 45.0%
ACACCCTCTTGATCTCATCA+AGG - chr8.1:36212511-36212530 MS.gene065200:CDS 45.0%
ACTTGTCAACGGCAAAGTAC+CGG + chr8.1:36212810-36212829 None:intergenic 45.0%
ATGAATATGAAGGTGGAGGC+CGG - chr8.1:36213208-36213227 MS.gene065200:CDS 45.0%
ATTAGCATTCCAACCCGGTT+CGG - chr8.1:36212589-36212608 MS.gene065200:CDS 45.0%
CGAACCTTGATGAGATCAAG+AGG + chr8.1:36212518-36212537 None:intergenic 45.0%
CGGAAAAACTAGCTGTCACA+TGG + chr8.1:36213132-36213151 None:intergenic 45.0%
CGGTGGATGTGATTAAGACT+AGG - chr8.1:36213182-36213201 MS.gene065200:CDS 45.0%
GCATAGCTTCCATCATTGCA+GGG - chr8.1:36212480-36212499 MS.gene065200:CDS 45.0%
GGGTCCTATGGCTCTTTATA+AGG - chr8.1:36213288-36213307 MS.gene065200:CDS 45.0%
GGTGATGAATATGAAGGTGG+AGG - chr8.1:36213204-36213223 MS.gene065200:CDS 45.0%
GTTAAGCTAGTCCAACAGGA+AGG - chr8.1:36212671-36212690 MS.gene065200:CDS 45.0%
GTTGGAATGCTAATGCTGGT+CGG + chr8.1:36212583-36212602 None:intergenic 45.0%
TGACAAGTAAGATTGGAGCG+GGG - chr8.1:36212822-36212841 MS.gene065200:CDS 45.0%
TGCTAATGCTGGTCGGATAT+TGG + chr8.1:36212576-36212595 None:intergenic 45.0%
TGTTGAACACCCTGCAATGA+TGG + chr8.1:36212492-36212511 None:intergenic 45.0%
TTGAAGACTGTTCGTGCTGA+GGG - chr8.1:36213268-36213287 MS.gene065200:CDS 45.0%
TTTGAAGACTGTTCGTGCTG+AGG - chr8.1:36213267-36213286 MS.gene065200:CDS 45.0%
! TAGTTTTTCCGCGGGTTTTG+TGG - chr8.1:36213141-36213160 MS.gene065200:CDS 45.0%
! TGGTGTTAAGCTAGTCCAAC+AGG - chr8.1:36212667-36212686 MS.gene065200:CDS 45.0%
!!! GGTGTAGCAGCACTTTTTTC+CGG - chr8.1:36212692-36212711 MS.gene065200:CDS 45.0%
!!! TGTGACAGCTAGTTTTTCCG+CGG - chr8.1:36213132-36213151 MS.gene065200:CDS 45.0%
AGCTTAACACCAACGGCGAT+CGG + chr8.1:36212659-36212678 None:intergenic 50.0%
ATAGAGACACTGCCGGAGAA+CGG + chr8.1:36212729-36212748 None:intergenic 50.0%
ATCATAGAGTCCCATACGGG+TGG + chr8.1:36212756-36212775 None:intergenic 50.0%
CAAAGCACAATCAATGGCGC+CGG + chr8.1:36213251-36213270 None:intergenic 50.0%
CCAGCATTAGCATTCCAACC+CGG - chr8.1:36212584-36212603 MS.gene065200:CDS 50.0%
CTAACCATTGCCACATCAGC+CGG + chr8.1:36212884-36212903 None:intergenic 50.0%
CTCTATTCCACCACCCGTAT+GGG - chr8.1:36212743-36212762 MS.gene065200:CDS 50.0%
GGCATAGCTTCCATCATTGC+AGG - chr8.1:36212479-36212498 MS.gene065200:CDS 50.0%
GGTCCCTGCCTTGTAATTGT+AGG + chr8.1:36213322-36213341 None:intergenic 50.0%
TAGTTTCTTCTCTGGGCCGA+TGG + chr8.1:36212929-36212948 None:intergenic 50.0%
TCTCTATTCCACCACCCGTA+TGG - chr8.1:36212742-36212761 MS.gene065200:CDS 50.0%
TGGTGGAATAGAGACACTGC+CGG + chr8.1:36212736-36212755 None:intergenic 50.0%
TGTGGCAATGGTTAGGATGC+AGG - chr8.1:36212889-36212908 MS.gene065200:CDS 50.0%
TTAAGGATGAACCGCGCCAT+AGG + chr8.1:36213006-36213025 None:intergenic 50.0%
TTGTGACGCCGTAACTAGCA+TGG + chr8.1:36213041-36213060 None:intergenic 50.0%
! ACGAGGGAGTTACTAGCCTA+TGG - chr8.1:36212987-36213006 MS.gene065200:CDS 50.0%
! GCTGGTCGGATATTGGTTGT+AGG + chr8.1:36212569-36212588 None:intergenic 50.0%
! GTTGGATGAAGGATGGGCTT+GGG - chr8.1:36213104-36213123 MS.gene065200:CDS 50.0%
!! AACGGCAAAGTACCGGAGAT+AGG + chr8.1:36212803-36212822 None:intergenic 50.0%
!! TTTTTCCGCGGGTTTTGTGG+CGG - chr8.1:36213144-36213163 MS.gene065200:CDS 50.0%
!!! GTGACAGCTAGTTTTTCCGC+GGG - chr8.1:36213133-36213152 MS.gene065200:CDS 50.0%
ACCAGGATGGCGAAAGACGA+GGG - chr8.1:36212971-36212990 MS.gene065200:CDS 55.0%
ATAGAGTCCCATACGGGTGG+TGG + chr8.1:36212753-36212772 None:intergenic 55.0%
ATGGTTAGGATGCAGGCCGA+CGG - chr8.1:36212896-36212915 MS.gene065200:CDS 55.0%
CATGGCGCGGTTCACTGTTA+AGG + chr8.1:36213023-36213042 None:intergenic 55.0%
CGGCTGATGTGGCAATGGTT+AGG - chr8.1:36212882-36212901 MS.gene065200:CDS 55.0%
CGTCGATGCTATCACCAGGA+TGG - chr8.1:36212958-36212977 MS.gene065200:CDS 55.0%
GAACCGCGCCATGCTAGTTA+CGG - chr8.1:36213030-36213049 MS.gene065200:CDS 55.0%
GAATCCGGCTGATGTGGCAA+TGG - chr8.1:36212877-36212896 MS.gene065200:CDS 55.0%
GCTAGTTACGGCGTCACAAC+TGG - chr8.1:36213042-36213061 MS.gene065200:CDS 55.0%
GGTGTGAAGGGTTTCGTCGA+AGG - chr8.1:36212455-36212474 MS.gene065200:CDS 55.0%
GTGAAGGGTTTCGTCGAAGG+AGG - chr8.1:36212458-36212477 MS.gene065200:CDS 55.0%
TGTTCGTGCTGAGGGTCCTA+TGG - chr8.1:36213276-36213295 MS.gene065200:CDS 55.0%
! CAATCAATGGCGCCGGAGTA+AGG + chr8.1:36213244-36213263 None:intergenic 55.0%
! CCGGGTTGGAATGCTAATGC+TGG + chr8.1:36212587-36212606 None:intergenic 55.0%
! GGAGTTACTAGCCTATGGCG+CGG - chr8.1:36212992-36213011 MS.gene065200:CDS 55.0%
! GGTTGGATGAAGGATGGGCT+TGG - chr8.1:36213103-36213122 MS.gene065200:CDS 55.0%
! GTGCTGCTACACCTTCCTGT+TGG + chr8.1:36212685-36212704 None:intergenic 55.0%
!! CCTGGTGATAGCATCGACGA+CGG + chr8.1:36212957-36212976 None:intergenic 55.0%
!! TCCAACCCGGTTCGGTTCAT+CGG - chr8.1:36212597-36212616 MS.gene065200:CDS 55.0%
ACCGATGAACCGAACCGGGT+TGG + chr8.1:36212601-36212620 None:intergenic 60.0%
ACGCCGTAACTAGCATGGCG+CGG + chr8.1:36213036-36213055 None:intergenic 60.0%
CACCAGGATGGCGAAAGACG+AGG - chr8.1:36212970-36212989 MS.gene065200:CDS 60.0%
CCGTCGTCGATGCTATCACC+AGG - chr8.1:36212954-36212973 MS.gene065200:CDS 60.0%
CGGTGACCGATGAACCGAAC+CGG + chr8.1:36212606-36212625 None:intergenic 60.0%
GAGAACGGTGGCAGAGACAC+CGG + chr8.1:36212714-36212733 None:intergenic 60.0%
GGAGTAAGGTGGAGGCGATC+CGG + chr8.1:36213230-36213249 None:intergenic 60.0%
GGATCGCCTCCACCTTACTC+CGG - chr8.1:36213229-36213248 MS.gene065200:CDS 60.0%
GGCCGACGGAAGACTTCCAT+CGG - chr8.1:36212910-36212929 MS.gene065200:CDS 60.0%
GGCCGATGGAAGTCTTCCGT+CGG + chr8.1:36212915-36212934 None:intergenic 60.0%
GGTGACCGATGAACCGAACC+GGG + chr8.1:36212605-36212624 None:intergenic 60.0%
GTGTCTCTGCCACCGTTCTC+CGG - chr8.1:36212714-36212733 MS.gene065200:CDS 60.0%
TCCCTCGTCTTTCGCCATCC+TGG + chr8.1:36212975-36212994 None:intergenic 60.0%
TGTCGGGAATCCGGCTGATG+TGG - chr8.1:36212871-36212890 MS.gene065200:CDS 60.0%
! TCAATGGCGCCGGAGTAAGG+TGG + chr8.1:36213241-36213260 None:intergenic 60.0%
! TCATCGGTCACCGGCTGTGA+CGG - chr8.1:36212613-36212632 MS.gene065200:CDS 60.0%
!! CGGTTCGGTTCATCGGTCAC+CGG - chr8.1:36212604-36212623 MS.gene065200:CDS 60.0%
AACCGCCGCCACAAAACCCG+CGG + chr8.1:36213152-36213171 None:intergenic 65.0%
AACGGCGATCGGCCCAACAC+GGG + chr8.1:36212648-36212667 None:intergenic 65.0%
CAACGGCGATCGGCCCAACA+CGG + chr8.1:36212649-36212668 None:intergenic 65.0%
GAGACACTGCCGGAGAACGG+TGG + chr8.1:36212726-36212745 None:intergenic 65.0%
! ATGGCGCCGGAGTAAGGTGG+AGG + chr8.1:36213238-36213257 None:intergenic 65.0%
!! TTCCGCGGGTTTTGTGGCGG+CGG - chr8.1:36213147-36213166 MS.gene065200:CDS 65.0%
ACGGCGATCGGCCCAACACG+GGG + chr8.1:36212647-36212666 None:intergenic 70.0%
ATTGGAGCGGGGCTGCTCGC+CGG - chr8.1:36212833-36212852 MS.gene065200:CDS 70.0%
CGTGTTGGGCCGATCGCCGT+TGG - chr8.1:36212647-36212666 MS.gene065200:CDS 70.0%
GACAGCCGCGCCAATCCCAC+CGG + chr8.1:36212855-36212874 None:intergenic 70.0%
GGCGGCGGTTGCGTCGAATC+CGG - chr8.1:36213162-36213181 MS.gene065200:CDS 70.0%
GGCGGTTGCGTCGAATCCGG+TGG - chr8.1:36213165-36213184 MS.gene065200:CDS 70.0%
TGGCGCGGCTGTCGGGAATC+CGG - chr8.1:36212862-36212881 MS.gene065200:CDS 70.0%
! GGTGGGATTGGCGCGGCTGT+CGG - chr8.1:36212854-36212873 MS.gene065200:CDS 70.0%
! GTGGGATTGGCGCGGCTGTC+GGG - chr8.1:36212855-36212874 MS.gene065200:CDS 70.0%
ACGGCCCAACCGCCCCGTGT+TGG - chr8.1:36212632-36212651 MS.gene065200:CDS 75.0%
CGGCCCAACACGGGGCGGTT+GGG + chr8.1:36212639-36212658 None:intergenic 75.0%
CGGCCCAACCGCCCCGTGTT+GGG - chr8.1:36212633-36212652 MS.gene065200:CDS 75.0%
GCGATCGGCCCAACACGGGG+CGG + chr8.1:36212644-36212663 None:intergenic 75.0%
GGCGGTTGGGCCGTCACAGC+CGG + chr8.1:36212626-36212645 None:intergenic 75.0%
GGGCTGCTCGCCGGTGGGAT+TGG - chr8.1:36212842-36212861 MS.gene065200:CDS 75.0%
TCGGCCCAACACGGGGCGGT+TGG + chr8.1:36212640-36212659 None:intergenic 75.0%
! GCTCGCCGGTGGGATTGGCG+CGG - chr8.1:36212847-36212866 MS.gene065200:CDS 75.0%
! GAGCGGGGCTGCTCGCCGGT+GGG - chr8.1:36212837-36212856 MS.gene065200:CDS 80.0%
! GGAGCGGGGCTGCTCGCCGG+TGG - chr8.1:36212836-36212855 MS.gene065200:CDS 85.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 36212452 36213402 36212452 ID=MS.gene065200
chr8.1 mRNA 36212452 36213402 36212452 ID=MS.gene065200.t1;Parent=MS.gene065200
chr8.1 exon 36212452 36213402 36212452 ID=MS.gene065200.t1.exon1;Parent=MS.gene065200.t1
chr8.1 CDS 36212452 36213402 36212452 ID=cds.MS.gene065200.t1;Parent=MS.gene065200.t1
Gene Sequence

>MS.gene065200

ATGGGTGTGAAGGGTTTCGTCGAAGGAGGCATAGCTTCCATCATTGCAGGGTGTTCAACACACCCTCTTGATCTCATCAAGGTTCGAATGCAGCTTCAAGGAGAGAATAATACTCCTACAACCAATATCCGACCAGCATTAGCATTCCAACCCGGTTCGGTTCATCGGTCACCGGCTGTGACGGCCCAACCGCCCCGTGTTGGGCCGATCGCCGTTGGTGTTAAGCTAGTCCAACAGGAAGGTGTAGCAGCACTTTTTTCCGGTGTCTCTGCCACCGTTCTCCGGCAGTGTCTCTATTCCACCACCCGTATGGGACTCTATGATATGATGAAGAAAAAATGGTCTGATCCTATCTCCGGTACTTTGCCGTTGACAAGTAAGATTGGAGCGGGGCTGCTCGCCGGTGGGATTGGCGCGGCTGTCGGGAATCCGGCTGATGTGGCAATGGTTAGGATGCAGGCCGACGGAAGACTTCCATCGGCCCAGAGAAGAAACTATAAGTCCGTCGTCGATGCTATCACCAGGATGGCGAAAGACGAGGGAGTTACTAGCCTATGGCGCGGTTCATCCTTAACAGTGAACCGCGCCATGCTAGTTACGGCGTCACAACTGGCATCCTATGATGAGTTTAAGGAAAAGATTTTAAAAAATGGTTGGATGAAGGATGGGCTTGGGACCCATGTGACAGCTAGTTTTTCCGCGGGTTTTGTGGCGGCGGTTGCGTCGAATCCGGTGGATGTGATTAAGACTAGGGTGATGAATATGAAGGTGGAGGCCGGATCGCCTCCACCTTACTCCGGCGCCATTGATTGTGCTTTGAAGACTGTTCGTGCTGAGGGTCCTATGGCTCTTTATAAGGGTTTCATTCCTACAATTACAAGGCAGGGACCGTTTACCGTTGTGTTGTTTGTTACATTGGAACAGGTTCGTAAGTTGCTTAAGGATTTCTAA
Protein sequence

>MS.gene065200.t1

MGVKGFVEGGIASIIAGCSTHPLDLIKVRMQLQGENNTPTTNIRPALAFQPGSVHRSPAVTAQPPRVGPIAVGVKLVQQEGVAALFSGVSATVLRQCLYSTTRMGLYDMMKKKWSDPISGTLPLTSKIGAGLLAGGIGAAVGNPADVAMVRMQADGRLPSAQRRNYKSVVDAITRMAKDEGVTSLWRGSSLTVNRAMLVTASQLASYDEFKEKILKNGWMKDGLGTHVTASFSAGFVAAVASNPVDVIKTRVMNMKVEAGSPPPYSGAIDCALKTVRAEGPMALYKGFIPTITRQGPFTVVLFVTLEQVRKLLKDF