AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065349

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 120 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAATCCAGCAACTATTTA+TGG 0.069649 2.4:-46834349 None:intergenic
AGAAAGCACTTGGTCTTATT+AGG 0.215397 2.4:-46834205 None:intergenic
AAGGAGTTCTATTCTGAAAT+TGG 0.237633 2.4:+46835091 MS.gene065349:CDS
TTGCAGTTAAGAGATTAAAA+AGG 0.259648 2.4:+46835050 MS.gene065349:CDS
AAGAGTGACGTTTATAGTTT+TGG 0.282002 2.4:+46836184 MS.gene065349:CDS
GTTTATGAGTTTGTAGCTAA+TGG 0.285632 2.4:+46835191 MS.gene065349:intron
TGAACAACGTGTGACATTCT+AGG 0.318462 2.4:-46836439 None:intergenic
GAACCGGACAATTCTGTCTT+AGG 0.336637 2.4:+46834481 MS.gene065349:CDS
CTAATGGGCCCTTAGATAAA+TGG 0.351205 2.4:+46835207 MS.gene065349:CDS
GTGATGTAGCCATTTATCTA+AGG 0.359817 2.4:-46835216 None:intergenic
GGCAAGCTTTAAGAGGAAAA+AGG 0.361076 2.4:+46835072 MS.gene065349:CDS
ATATAAGGATCCAAGAGTTC+AGG 0.364706 2.4:-46836319 None:intergenic
GGAGTTTGGAGCACACCTTA+TGG 0.365447 2.4:+46836045 MS.gene065349:CDS
AGAGACATGTTGTAATAATA+TGG 0.365925 2.4:+46834261 MS.gene065349:CDS
AACATTAGCCAAAGGGAATA+TGG 0.372680 2.4:-46834442 None:intergenic
TTTATGAGTTTGTAGCTAAT+GGG 0.377084 2.4:+46835192 MS.gene065349:intron
GACATTCTAGGGCGCATAGA+AGG 0.377991 2.4:-46836427 None:intergenic
ATGCCCAAATGGTTGGGTTA+TGG 0.380103 2.4:+46834180 MS.gene065349:CDS
CCACTTAATTGTACCGGAAC+CGG 0.382108 2.4:+46834465 MS.gene065349:CDS
CAACATTAGCAGTAGTTGTA+TGG 0.382483 2.4:+46834829 MS.gene065349:CDS
CGCTTCATATGGCACAAACT+TGG 0.386602 2.4:-46836080 None:intergenic
CCATCACAAACCACCAGGTT+TGG 0.391511 2.4:-46835136 None:intergenic
GAAAGCACTTGGTCTTATTA+GGG 0.398585 2.4:-46834204 None:intergenic
AAGACAGAATTGTCCGGTTC+CGG 0.400139 2.4:-46834478 None:intergenic
GGTCCATAACCCAACCATTT+GGG 0.401462 2.4:-46834183 None:intergenic
GAGTTTGGAGCACACCTTAT+GGG 0.406213 2.4:+46836046 MS.gene065349:CDS
GGGGAGGTCGCAGCTTAGAT+TGG 0.408202 2.4:+46835246 MS.gene065349:CDS
GTGCTGCTGGACGAGGAGTT+TGG 0.414569 2.4:+46836031 MS.gene065349:CDS
GTAGATGAGGAAGATATATA+AGG 0.419173 2.4:-46836334 None:intergenic
AGACAAGGTCAACCACCTTC+TGG 0.419954 2.4:-46836384 None:intergenic
GGGTCCATAACCCAACCATT+TGG 0.423562 2.4:-46834184 None:intergenic
CTCTCATGCATCGCTTCATA+TGG 0.423953 2.4:-46836091 None:intergenic
TCTTTGTTGTAAGCTCGTTT+CGG 0.431786 2.4:-46836165 None:intergenic
CCAAGTGCTTTCTCCATGTT+GGG 0.434270 2.4:+46834215 MS.gene065349:CDS
GAATAAGTCCATATTCCCTT+TGG 0.434737 2.4:+46834434 MS.gene065349:CDS
AGTTCTATTCTGAAATTGGC+AGG 0.439600 2.4:+46835095 MS.gene065349:CDS
AGTGAAGGAAACCGCCTTGT+TGG 0.452771 2.4:+46834975 MS.gene065349:CDS
CTACTACTCGAAATAGTTAG+TGG 0.456858 2.4:+46836211 MS.gene065349:CDS
GTTGGCAGAGTATATTTGAA+TGG 0.459965 2.4:+46836266 MS.gene065349:CDS
GGAGCACACCTTATGGGTGT+TGG 0.462484 2.4:+46836052 MS.gene065349:CDS
ACCAAGTGCTTTCTCCATGT+TGG 0.466801 2.4:+46834214 MS.gene065349:CDS
GTGAATCCATAAATAGTTGC+TGG 0.473692 2.4:+46834343 MS.gene065349:CDS
TTACCACTGCAAGGATGATA+AGG 0.474079 2.4:-46834788 None:intergenic
TCGAGCTAGTAATGTGCTGC+TGG 0.476115 2.4:+46836018 MS.gene065349:CDS
CTGAAATTGGCAGGGTTGCA+AGG 0.476585 2.4:+46835104 MS.gene065349:CDS
ATGGCTTCTTGTATATAAGC+AGG 0.478581 2.4:+46834848 MS.gene065349:CDS
TCATCGCTACCCTGAACTCT+TGG 0.480687 2.4:+46836309 MS.gene065349:CDS
CATGAGAGGACTGTGATGGC+AGG 0.482521 2.4:+46836106 MS.gene065349:CDS
AGCACCATGCCCAAATGGTT+GGG 0.487078 2.4:+46834174 None:intergenic
CCGGTTCCGGTACAATTAAG+TGG 0.490246 2.4:-46834465 None:intergenic
AGACTCAACAACACTATGTC+GGG 0.496446 2.4:+46834378 MS.gene065349:CDS
TTGGCAGAGTATATTTGAAT+GGG 0.503697 2.4:+46836267 MS.gene065349:CDS
GGGAGGTCGCAGCTTAGATT+GGG 0.507395 2.4:+46835247 MS.gene065349:CDS
CCCAACATGGAGAAAGCACT+TGG 0.508122 2.4:-46834215 None:intergenic
ATCATCCTTGCAGTGGTAAG+TGG 0.508626 2.4:+46834792 MS.gene065349:CDS
GAACAACGTGTGACATTCTA+GGG 0.509502 2.4:-46836438 None:intergenic
TAGTAATGTGCTGCTGGACG+AGG 0.511455 2.4:+46836024 MS.gene065349:CDS
ATGGAAAATCTTTACAAAAG+AGG 0.512490 2.4:+46834926 MS.gene065349:CDS
TCTTTACAAAAGAGGAACTA+AGG 0.513394 2.4:+46834934 MS.gene065349:CDS
ATGTTGGGAGGCCTCAATCA+TGG 0.517825 2.4:+46834230 MS.gene065349:CDS
TCTCTATGTATACTTGACTT+AGG 0.520787 2.4:+46834585 MS.gene065349:CDS
TCCATAAATAGTTGCTGGAT+TGG 0.523670 2.4:+46834348 MS.gene065349:CDS
TATAAGGATCCAAGAGTTCA+GGG 0.526939 2.4:-46836318 None:intergenic
ATAAATAGTTGCTGGATTGG+TGG 0.527869 2.4:+46834351 MS.gene065349:CDS
AATGTTCCACTTAATTGTAC+CGG 0.527889 2.4:+46834459 MS.gene065349:CDS
CCTTGTTGGAGATGCAAGAC+TGG 0.531401 2.4:+46834989 MS.gene065349:CDS
TGTGCAGCACCATGCCCAAA+TGG 0.532948 2.4:+46834169 None:intergenic
TCTGAGTCATTCCATGATTG+AGG 0.533121 2.4:-46834241 None:intergenic
CACTAACTATTTCGAGTAGT+AGG 0.534554 2.4:-46836210 None:intergenic
TGATGTAGCCATTTATCTAA+GGG 0.539000 2.4:-46835215 None:intergenic
CTGCTGCTACACTTCCATCA+TGG 0.540207 2.4:+46834907 MS.gene065349:CDS
TTCTGGATAGTACTAGTCTC+TGG 0.544812 2.4:-46836367 None:intergenic
TAAGTGGAACATTAGCCAAA+GGG 0.545922 2.4:-46834449 None:intergenic
GTAATCATATGCACTGCCAT+AGG 0.545967 2.4:+46834760 MS.gene065349:CDS
TGGACAAGCCAACACCCATA+AGG 0.547153 2.4:-46836060 None:intergenic
GCAGCTTAGATTGGGCTATG+AGG 0.548458 2.4:+46835255 MS.gene065349:CDS
TCTAAGCTGCGACCTCCCCT+TGG 0.552914 2.4:-46835242 None:intergenic
CAGCACCATGCCCAAATGGT+TGG 0.556216 2.4:+46834173 None:intergenic
ACAGAATCAACTGCTTGTGC+TGG 0.557567 2.4:-46836241 None:intergenic
AAGCAGTTGATTCTGTGGGT+TGG 0.558849 2.4:+46836248 MS.gene065349:CDS
CATGATGGAAGTGTAGCAGC+AGG 0.561796 2.4:-46834906 None:intergenic
TAATCATATGCACTGCCATA+GGG 0.562277 2.4:+46834761 MS.gene065349:CDS
ATTGCAACGACGCTTGCTCA+AGG 0.562585 2.4:+46835284 MS.gene065349:CDS
GGATGATAAGGTATTCCCTA+TGG 0.566165 2.4:-46834776 None:intergenic
ACATTCTAGGGCGCATAGAA+GGG 0.566800 2.4:-46836426 None:intergenic
GTTCCTAAGACAGAATTGTC+CGG 0.569173 2.4:-46834484 None:intergenic
ATGATTGAGGCCTCCCAACA+TGG 0.578662 2.4:-46834228 None:intergenic
TCGTTTCGGTACACAAACTC+TGG 0.578686 2.4:-46836151 None:intergenic
AGCACAAGCAGTTGATTCTG+TGG 0.580618 2.4:+46836243 MS.gene065349:CDS
AAGACTCAACAACACTATGT+CGG 0.583057 2.4:+46834377 MS.gene065349:CDS
AGTGCTTTCTCCATGTTGGG+AGG 0.583986 2.4:+46834218 MS.gene065349:CDS
TAGCGATGAGCTTGAACAAG+AGG 0.587250 2.4:-46836295 None:intergenic
ATGAGAGGACTGTGATGGCA+GGG 0.589701 2.4:+46836107 MS.gene065349:CDS
ATATCTCGATGAACAACTTG+TGG 0.590367 2.4:-46835995 None:intergenic
CATATGAAGCGATGCATGAG+AGG 0.590509 2.4:+46836092 MS.gene065349:CDS
TTAAGTGGAACATTAGCCAA+AGG 0.596276 2.4:-46834450 None:intergenic
TGAGAGGACTGTGATGGCAG+GGG 0.596780 2.4:+46836108 MS.gene065349:CDS
TAAGTCAAGTATACATAGAG+AGG 0.599321 2.4:-46834583 None:intergenic
GCACAAGCAGTTGATTCTGT+GGG 0.611876 2.4:+46836244 MS.gene065349:CDS
AAGGCTTCGACATCCAAACC+TGG 0.613833 2.4:+46835123 MS.gene065349:CDS
TGGCTTCTTGTATATAAGCA+GGG 0.613910 2.4:+46834849 MS.gene065349:CDS
AATGGCTACATCACATACCA+AGG 0.615578 2.4:+46835225 MS.gene065349:CDS
TGGTATGATACTAGTAGATG+AGG 0.621129 2.4:-46836347 None:intergenic
GAGAGGACTGTGATGGCAGG+GGG 0.623889 2.4:+46836109 MS.gene065349:CDS
CCAGTCTTGCATCTCCAACA+AGG 0.628420 2.4:-46834989 None:intergenic
TCACTCCACTTACCACTGCA+AGG 0.631760 2.4:-46834797 None:intergenic
ATACCTTATCATCCTTGCAG+TGG 0.635282 2.4:+46834785 MS.gene065349:CDS
GTTCTATTCTGAAATTGGCA+GGG 0.635807 2.4:+46835096 MS.gene065349:CDS
ATGGCTACATCACATACCAA+GGG 0.641272 2.4:+46835226 MS.gene065349:CDS
ATAACCCAACCATTTGGGCA+TGG 0.642347 2.4:-46834178 None:intergenic
GACTAGTACTATCCAGAAGG+TGG 0.651449 2.4:+46836372 MS.gene065349:CDS
AGAGACTAGTACTATCCAGA+AGG 0.665219 2.4:+46836369 MS.gene065349:CDS
CATGGTCTGATGATTCACAA+TGG 0.687957 2.4:+46834412 MS.gene065349:CDS
GATGCATGAGAGGACTGTGA+TGG 0.695690 2.4:+46836102 MS.gene065349:CDS
GCTGCGTGCATGAATAGACA+AGG 0.705355 2.4:-46836399 None:intergenic
GCTTCGACATCCAAACCTGG+TGG 0.706271 2.4:+46835126 MS.gene065349:CDS
AGTATACATAGAGAGGCATG+AGG 0.707905 2.4:-46834576 None:intergenic
CTACATCACATACCAAGGGG+AGG 0.714671 2.4:+46835230 MS.gene065349:CDS
GTCTTGCATCTCCAACAAGG+CGG 0.719523 2.4:-46834986 None:intergenic
TGGCTACATCACATACCAAG+GGG 0.729491 2.4:+46835227 MS.gene065349:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATATTTATTACATAAGA+CGG - chr2.4:46835625-46835644 None:intergenic 15.0%
!! ATTTAATTCATTCTTTCAAA+AGG + chr2.4:46834686-46834705 MS.gene065349:intron 15.0%
!! TATGAGAGAATAATTCTAAA+TGG - chr2.4:46835551-46835570 None:intergenic 20.0%
!! TGATTTACTAACAAGAAAAA+AGG + chr2.4:46835872-46835891 MS.gene065349:intron 20.0%
!!! AAGAAATACTTTTGTTTGAA+GGG - chr2.4:46835789-46835808 None:intergenic 20.0%
!!! ACATTTTGTATCTGAAAAAA+TGG - chr2.4:46834742-46834761 None:intergenic 20.0%
!!! TAAGAAATACTTTTGTTTGA+AGG - chr2.4:46835790-46835809 None:intergenic 20.0%
! AAAAAAAACAAACTTGCGTA+TGG - chr2.4:46835393-46835412 None:intergenic 25.0%
! ACATGTTGTAATAATATGGT+GGG + chr2.4:46834265-46834284 MS.gene065349:intron 25.0%
! AGAGACATGTTGTAATAATA+TGG + chr2.4:46834261-46834280 MS.gene065349:CDS 25.0%
! ATGGAAAATCTTTACAAAAG+AGG + chr2.4:46834926-46834945 MS.gene065349:CDS 25.0%
! ATTACGAAAAATTTCAGTGA+AGG + chr2.4:46834960-46834979 MS.gene065349:CDS 25.0%
! GAAAATCTTGAAAACATACA+AGG - chr2.4:46835680-46835699 None:intergenic 25.0%
! TGATGTGATTTATAATCCAA+TGG - chr2.4:46835416-46835435 None:intergenic 25.0%
! TGCAGTTAAGAGATTAAAAA+GGG + chr2.4:46835051-46835070 MS.gene065349:CDS 25.0%
! TTAGTAAATCAAACTCGTAA+AGG - chr2.4:46835863-46835882 None:intergenic 25.0%
! TTGCAGTTAAGAGATTAAAA+AGG + chr2.4:46835050-46835069 MS.gene065349:CDS 25.0%
! TTTATGAGTTTGTAGCTAAT+GGG + chr2.4:46835192-46835211 MS.gene065349:intron 25.0%
!! TTTGTAAAGATTTTCCATGA+TGG - chr2.4:46834924-46834943 None:intergenic 25.0%
!!! CAAGTTTGTTTTTTTTCCAT+TGG + chr2.4:46835397-46835416 MS.gene065349:intron 25.0%
AAATCAGAAAGTCTTAGCAT+TGG - chr2.4:46835452-46835471 None:intergenic 30.0%
AATGTAAAATGAGAATCCGT+GGG - chr2.4:46834630-46834649 None:intergenic 30.0%
AATGTTCCACTTAATTGTAC+CGG + chr2.4:46834459-46834478 MS.gene065349:CDS 30.0%
CAAACTCATAAACAATGTAG+CGG - chr2.4:46835184-46835203 None:intergenic 30.0%
GACATGTTGTAATAATATGG+TGG + chr2.4:46834264-46834283 MS.gene065349:intron 30.0%
GCTAAGACTTTCTGATTTAA+CGG + chr2.4:46835454-46835473 MS.gene065349:intron 30.0%
GTAGATGAGGAAGATATATA+AGG - chr2.4:46836337-46836356 None:intergenic 30.0%
GTTTATGAGTTTGTAGCTAA+TGG + chr2.4:46835191-46835210 MS.gene065349:intron 30.0%
TAAGTCAAGTATACATAGAG+AGG - chr2.4:46834586-46834605 None:intergenic 30.0%
TCTAAATGGACATGTAGAAA+GGG - chr2.4:46835537-46835556 None:intergenic 30.0%
TCTCTATGTATACTTGACTT+AGG + chr2.4:46834585-46834604 MS.gene065349:CDS 30.0%
TCTTTACAAAAGAGGAACTA+AGG + chr2.4:46834934-46834953 MS.gene065349:CDS 30.0%
TGATGTAGCCATTTATCTAA+GGG - chr2.4:46835218-46835237 None:intergenic 30.0%
TTCTAAATGGACATGTAGAA+AGG - chr2.4:46835538-46835557 None:intergenic 30.0%
TTGGCAGAGTATATTTGAAT+GGG + chr2.4:46836267-46836286 MS.gene065349:CDS 30.0%
! AAGAGTGACGTTTATAGTTT+TGG + chr2.4:46836184-46836203 MS.gene065349:CDS 30.0%
!! AAGGAGTTCTATTCTGAAAT+TGG + chr2.4:46835091-46835110 MS.gene065349:CDS 30.0%
AAACTTGATACCCATTTCCA+GGG + chr2.4:46835706-46835725 MS.gene065349:intron 35.0%
AACATTAGCCAAAGGGAATA+TGG - chr2.4:46834445-46834464 None:intergenic 35.0%
AAGACTCAACAACACTATGT+CGG + chr2.4:46834377-46834396 MS.gene065349:CDS 35.0%
AAGACTTTCTGATTTAACGG+TGG + chr2.4:46835457-46835476 MS.gene065349:intron 35.0%
ACCAATCCAGCAACTATTTA+TGG - chr2.4:46834352-46834371 None:intergenic 35.0%
ATATAAGGATCCAAGAGTTC+AGG - chr2.4:46836322-46836341 None:intergenic 35.0%
ATATCTCGATGAACAACTTG+TGG - chr2.4:46835998-46836017 None:intergenic 35.0%
ATGGCTTCTTGTATATAAGC+AGG + chr2.4:46834848-46834867 MS.gene065349:CDS 35.0%
CAACATTAGCAGTAGTTGTA+TGG + chr2.4:46834829-46834848 MS.gene065349:CDS 35.0%
CTACTACTCGAAATAGTTAG+TGG + chr2.4:46836211-46836230 MS.gene065349:CDS 35.0%
GAATAAGTCCATATTCCCTT+TGG + chr2.4:46834434-46834453 MS.gene065349:CDS 35.0%
GAATGTAAAATGAGAATCCG+TGG - chr2.4:46834631-46834650 None:intergenic 35.0%
GTGAATCCATAAATAGTTGC+TGG + chr2.4:46834343-46834362 MS.gene065349:CDS 35.0%
GTGATGTAGCCATTTATCTA+AGG - chr2.4:46835219-46835238 None:intergenic 35.0%
GTTGGCAGAGTATATTTGAA+TGG + chr2.4:46836266-46836285 MS.gene065349:CDS 35.0%
TAAGTGGAACATTAGCCAAA+GGG - chr2.4:46834452-46834471 None:intergenic 35.0%
TAATCATATGCACTGCCATA+GGG + chr2.4:46834761-46834780 MS.gene065349:CDS 35.0%
TATAAGGATCCAAGAGTTCA+GGG - chr2.4:46836321-46836340 None:intergenic 35.0%
TCCATAAATAGTTGCTGGAT+TGG + chr2.4:46834348-46834367 MS.gene065349:CDS 35.0%
TGAAACCCAACAAACATATG+TGG - chr2.4:46835734-46835753 None:intergenic 35.0%
TGGCTTCTTGTATATAAGCA+GGG + chr2.4:46834849-46834868 MS.gene065349:CDS 35.0%
TGGTATGATACTAGTAGATG+AGG - chr2.4:46836350-46836369 None:intergenic 35.0%
TGTTGGGTTTCACTTAGTTA+TGG + chr2.4:46835742-46835761 MS.gene065349:intron 35.0%
TTAAGTGGAACATTAGCCAA+AGG - chr2.4:46834453-46834472 None:intergenic 35.0%
! AGCCCATAGCATTTTAAACT+CGG + chr2.4:46835902-46835921 MS.gene065349:intron 35.0%
! ATAAATAGTTGCTGGATTGG+TGG + chr2.4:46834351-46834370 MS.gene065349:CDS 35.0%
! CACCGAGTTTAAAATGCTAT+GGG - chr2.4:46835907-46835926 None:intergenic 35.0%
! CACTAACTATTTCGAGTAGT+AGG - chr2.4:46836213-46836232 None:intergenic 35.0%
! GCTCTTGTTTATATTACCCA+CGG + chr2.4:46834611-46834630 MS.gene065349:intron 35.0%
! TCTTTGTTGTAAGCTCGTTT+CGG - chr2.4:46836168-46836187 None:intergenic 35.0%
!! AGAAAGCACTTGGTCTTATT+AGG - chr2.4:46834208-46834227 None:intergenic 35.0%
!! AGTTCTATTCTGAAATTGGC+AGG + chr2.4:46835095-46835114 MS.gene065349:CDS 35.0%
!! GAAAGCACTTGGTCTTATTA+GGG - chr2.4:46834207-46834226 None:intergenic 35.0%
!! GTTCTATTCTGAAATTGGCA+GGG + chr2.4:46835096-46835115 MS.gene065349:CDS 35.0%
!! TAAAAAGGGCAAGCTTTAAG+AGG + chr2.4:46835065-46835084 MS.gene065349:CDS 35.0%
!!! TTTTTTTCTCCCGTTTCAAC+AGG + chr2.4:46835951-46835970 MS.gene065349:intron 35.0%
AATGGCTACATCACATACCA+AGG + chr2.4:46835225-46835244 MS.gene065349:CDS 40.0%
AGACTCAACAACACTATGTC+GGG + chr2.4:46834378-46834397 MS.gene065349:CDS 40.0%
AGAGACTAGTACTATCCAGA+AGG + chr2.4:46836369-46836388 MS.gene065349:CDS 40.0%
ATACCTTATCATCCTTGCAG+TGG + chr2.4:46834785-46834804 MS.gene065349:CDS 40.0%
ATGCAGAAACCTGTTGAAAC+GGG - chr2.4:46835963-46835982 None:intergenic 40.0%
ATGGCTACATCACATACCAA+GGG + chr2.4:46835226-46835245 MS.gene065349:CDS 40.0%
CATGCAGAAACCTGTTGAAA+CGG - chr2.4:46835964-46835983 None:intergenic 40.0%
CATGGTCTGATGATTCACAA+TGG + chr2.4:46834412-46834431 MS.gene065349:CDS 40.0%
GAAACTTGATACCCATTTCC+AGG + chr2.4:46835705-46835724 MS.gene065349:intron 40.0%
GAACAACGTGTGACATTCTA+GGG - chr2.4:46836441-46836460 None:intergenic 40.0%
GTAATCATATGCACTGCCAT+AGG + chr2.4:46834760-46834779 MS.gene065349:CDS 40.0%
GTTCCTAAGACAGAATTGTC+CGG - chr2.4:46834487-46834506 None:intergenic 40.0%
TCTGAGTCATTCCATGATTG+AGG - chr2.4:46834244-46834263 None:intergenic 40.0%
TGAACAACGTGTGACATTCT+AGG - chr2.4:46836442-46836461 None:intergenic 40.0%
TGTAGAAAGGGCCAAATCTA+CGG - chr2.4:46835525-46835544 None:intergenic 40.0%
TTACCACTGCAAGGATGATA+AGG - chr2.4:46834791-46834810 None:intergenic 40.0%
! AGTATACATAGAGAGGCATG+AGG - chr2.4:46834579-46834598 None:intergenic 40.0%
! CATTTTGCTCAAAGTCTGTG+TGG + chr2.4:46834321-46834340 MS.gene065349:CDS 40.0%
! CCACCGAGTTTAAAATGCTA+TGG - chr2.4:46835908-46835927 None:intergenic 40.0%
! CCATAGCATTTTAAACTCGG+TGG + chr2.4:46835905-46835924 MS.gene065349:intron 40.0%
! CTAATGGGCCCTTAGATAAA+TGG + chr2.4:46835207-46835226 MS.gene065349:CDS 40.0%
! GGATGATAAGGTATTCCCTA+TGG - chr2.4:46834779-46834798 None:intergenic 40.0%
! TTCTGGATAGTACTAGTCTC+TGG - chr2.4:46836370-46836389 None:intergenic 40.0%
!! GGCAAGCTTTAAGAGGAAAA+AGG + chr2.4:46835072-46835091 MS.gene065349:CDS 40.0%
AACAAACATATGTGGTGCCC+TGG - chr2.4:46835726-46835745 None:intergenic 45.0%
AAGACAGAATTGTCCGGTTC+CGG - chr2.4:46834481-46834500 None:intergenic 45.0%
ACAGAATCAACTGCTTGTGC+TGG - chr2.4:46836244-46836263 None:intergenic 45.0%
ACCAAGTGCTTTCTCCATGT+TGG + chr2.4:46834214-46834233 MS.gene065349:CDS 45.0%
AGCATCATTCGCATCACTTC+AGG + chr2.4:46834293-46834312 MS.gene065349:intron 45.0%
ATAACCCAACCATTTGGGCA+TGG - chr2.4:46834181-46834200 None:intergenic 45.0%
ATATGTGGTGCCCTGGAAAT+GGG - chr2.4:46835719-46835738 None:intergenic 45.0%
ATCATCCTTGCAGTGGTAAG+TGG + chr2.4:46834792-46834811 MS.gene065349:CDS 45.0%
ATGCCCAAATGGTTGGGTTA+TGG + chr2.4:46834180-46834199 MS.gene065349:CDS 45.0%
ATGGGATGCTGCTATGATCA+TGG + chr2.4:46835155-46835174 MS.gene065349:intron 45.0%
CATATGAAGCGATGCATGAG+AGG + chr2.4:46836092-46836111 MS.gene065349:CDS 45.0%
CCAAATCTACGGTACACAAG+TGG - chr2.4:46835514-46835533 None:intergenic 45.0%
CCAAGTGCTTTCTCCATGTT+GGG + chr2.4:46834215-46834234 MS.gene065349:CDS 45.0%
CCACTTAATTGTACCGGAAC+CGG + chr2.4:46834465-46834484 MS.gene065349:CDS 45.0%
CCACTTGTGTACCGTAGATT+TGG + chr2.4:46835511-46835530 MS.gene065349:intron 45.0%
CGCTTCATATGGCACAAACT+TGG - chr2.4:46836083-46836102 None:intergenic 45.0%
CTCTCATGCATCGCTTCATA+TGG - chr2.4:46836094-46836113 None:intergenic 45.0%
GAACCGGACAATTCTGTCTT+AGG + chr2.4:46834481-46834500 MS.gene065349:CDS 45.0%
GACTAGTACTATCCAGAAGG+TGG + chr2.4:46836372-46836391 MS.gene065349:CDS 45.0%
GGGCACCACATATGTTTGTT+GGG + chr2.4:46835726-46835745 MS.gene065349:intron 45.0%
GGTCCATAACCCAACCATTT+GGG - chr2.4:46834186-46834205 None:intergenic 45.0%
TAGCGATGAGCTTGAACAAG+AGG - chr2.4:46836298-46836317 None:intergenic 45.0%
TCGTTTCGGTACACAAACTC+TGG - chr2.4:46836154-46836173 None:intergenic 45.0%
TGGCTACATCACATACCAAG+GGG + chr2.4:46835227-46835246 MS.gene065349:CDS 45.0%
! AAGCAGTTGATTCTGTGGGT+TGG + chr2.4:46836248-46836267 MS.gene065349:CDS 45.0%
! ACACTATGTCGGGTTTTCAG+TGG + chr2.4:46834388-46834407 MS.gene065349:CDS 45.0%
! AGCACAAGCAGTTGATTCTG+TGG + chr2.4:46836243-46836262 MS.gene065349:CDS 45.0%
! AGGGCACCACATATGTTTGT+TGG + chr2.4:46835725-46835744 MS.gene065349:intron 45.0%
! CAAACCTGGTGGTTTGTGAT+GGG + chr2.4:46835137-46835156 MS.gene065349:intron 45.0%
! GAGTTTGGAGCACACCTTAT+GGG + chr2.4:46836046-46836065 MS.gene065349:CDS 45.0%
! GCACAAGCAGTTGATTCTGT+GGG + chr2.4:46836244-46836263 MS.gene065349:CDS 45.0%
!! ACATTCTAGGGCGCATAGAA+GGG - chr2.4:46836429-46836448 None:intergenic 45.0%
!!! ATCTTTTTTATTTTATTTTT+GGG + chr2.4:46834712-46834731 MS.gene065349:intron 5.0%
!!! TATCTTTTTTATTTTATTTT+TGG + chr2.4:46834711-46834730 MS.gene065349:intron 5.0%
!!! TCTTTTTTTTTTTTTTTTTT+TGG + chr2.4:46835833-46835852 MS.gene065349:intron 5.0%
AAGACTGGTGGTACGTACAG+TGG + chr2.4:46835004-46835023 MS.gene065349:intron 50.0%
AAGGCTTCGACATCCAAACC+TGG + chr2.4:46835123-46835142 MS.gene065349:CDS 50.0%
AGACAAGGTCAACCACCTTC+TGG - chr2.4:46836387-46836406 None:intergenic 50.0%
AGACTGGTGGTACGTACAGT+GGG + chr2.4:46835005-46835024 MS.gene065349:intron 50.0%
AGTGAAGGAAACCGCCTTGT+TGG + chr2.4:46834975-46834994 MS.gene065349:CDS 50.0%
AGTGCTTTCTCCATGTTGGG+AGG + chr2.4:46834218-46834237 MS.gene065349:CDS 50.0%
ATGAGAGGACTGTGATGGCA+GGG + chr2.4:46836107-46836126 MS.gene065349:CDS 50.0%
ATGATTGAGGCCTCCCAACA+TGG - chr2.4:46834231-46834250 None:intergenic 50.0%
ATGTTGGGAGGCCTCAATCA+TGG + chr2.4:46834230-46834249 MS.gene065349:CDS 50.0%
ATTGCAACGACGCTTGCTCA+AGG + chr2.4:46835284-46835303 MS.gene065349:CDS 50.0%
CAACCTTTGAGCCATCAGCT+TGG - chr2.4:46835033-46835052 None:intergenic 50.0%
CATATGTGGTGCCCTGGAAA+TGG - chr2.4:46835720-46835739 None:intergenic 50.0%
CCAGTCTTGCATCTCCAACA+AGG - chr2.4:46834992-46835011 None:intergenic 50.0%
CCATCACAAACCACCAGGTT+TGG - chr2.4:46835139-46835158 None:intergenic 50.0%
CCGGTTCCGGTACAATTAAG+TGG - chr2.4:46834468-46834487 None:intergenic 50.0%
CCTTGTTGGAGATGCAAGAC+TGG + chr2.4:46834989-46835008 MS.gene065349:CDS 50.0%
CTACATCACATACCAAGGGG+AGG + chr2.4:46835230-46835249 MS.gene065349:CDS 50.0%
CTGAAATTGGCAGGGTTGCA+AGG + chr2.4:46835104-46835123 MS.gene065349:CDS 50.0%
CTGCTGCTACACTTCCATCA+TGG + chr2.4:46834907-46834926 MS.gene065349:CDS 50.0%
GATGCATGAGAGGACTGTGA+TGG + chr2.4:46836102-46836121 MS.gene065349:CDS 50.0%
GCAGCTTAGATTGGGCTATG+AGG + chr2.4:46835255-46835274 MS.gene065349:CDS 50.0%
GCTGCGTGCATGAATAGACA+AGG - chr2.4:46836402-46836421 None:intergenic 50.0%
GGGTCCATAACCCAACCATT+TGG - chr2.4:46834187-46834206 None:intergenic 50.0%
GTCTTGCATCTCCAACAAGG+CGG - chr2.4:46834989-46835008 None:intergenic 50.0%
TCACTCCACTTACCACTGCA+AGG - chr2.4:46834800-46834819 None:intergenic 50.0%
TCATCGCTACCCTGAACTCT+TGG + chr2.4:46836309-46836328 MS.gene065349:CDS 50.0%
TCGAGCTAGTAATGTGCTGC+TGG + chr2.4:46836018-46836037 MS.gene065349:CDS 50.0%
TGGACAAGCCAACACCCATA+AGG - chr2.4:46836063-46836082 None:intergenic 50.0%
TGTTGGAGATGCAAGACTGG+TGG + chr2.4:46834992-46835011 MS.gene065349:intron 50.0%
! CATGATGGAAGTGTAGCAGC+AGG - chr2.4:46834909-46834928 None:intergenic 50.0%
! CCAAACCTGGTGGTTTGTGA+TGG + chr2.4:46835136-46835155 MS.gene065349:intron 50.0%
! GGAGTTTGGAGCACACCTTA+TGG + chr2.4:46836045-46836064 MS.gene065349:CDS 50.0%
! TAGTAATGTGCTGCTGGACG+AGG + chr2.4:46836024-46836043 MS.gene065349:CDS 50.0%
! TGTCGGGTTTTCAGTGGACA+TGG + chr2.4:46834394-46834413 MS.gene065349:CDS 50.0%
!! CCCAACATGGAGAAAGCACT+TGG - chr2.4:46834218-46834237 None:intergenic 50.0%
!! GACATTCTAGGGCGCATAGA+AGG - chr2.4:46836430-46836449 None:intergenic 50.0%
CATGAGAGGACTGTGATGGC+AGG + chr2.4:46836106-46836125 MS.gene065349:CDS 55.0%
GACTGGTGGTACGTACAGTG+GGG + chr2.4:46835006-46835025 MS.gene065349:intron 55.0%
GCATCCCATCACAAACCACC+AGG - chr2.4:46835144-46835163 None:intergenic 55.0%
GCTTCGACATCCAAACCTGG+TGG + chr2.4:46835126-46835145 MS.gene065349:CDS 55.0%
GGAGCACACCTTATGGGTGT+TGG + chr2.4:46836052-46836071 MS.gene065349:CDS 55.0%
GGGAGGTCGCAGCTTAGATT+GGG + chr2.4:46835247-46835266 MS.gene065349:CDS 55.0%
GGTCCAAGCTGATGGCTCAA+AGG + chr2.4:46835027-46835046 MS.gene065349:intron 55.0%
GTGATGGCAGGGGGAACTTA+TGG + chr2.4:46836118-46836137 MS.gene065349:CDS 55.0%
TACAGTGGGGTCCAAGCTGA+TGG + chr2.4:46835019-46835038 MS.gene065349:intron 55.0%
TGAGAGGACTGTGATGGCAG+GGG + chr2.4:46836108-46836127 MS.gene065349:CDS 55.0%
GAGAGGACTGTGATGGCAGG+GGG + chr2.4:46836109-46836128 MS.gene065349:CDS 60.0%
GGGGAGGTCGCAGCTTAGAT+TGG + chr2.4:46835246-46835265 MS.gene065349:CDS 60.0%
TCTAAGCTGCGACCTCCCCT+TGG - chr2.4:46835245-46835264 None:intergenic 60.0%
! GTGCTGCTGGACGAGGAGTT+TGG + chr2.4:46836031-46836050 MS.gene065349:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 46834176 46836498 46834176 ID=MS.gene065349
chr2.4 mRNA 46834176 46836498 46834176 ID=MS.gene065349.t1;Parent=MS.gene065349
chr2.4 exon 46834176 46834282 46834176 ID=MS.gene065349.t1.exon1;Parent=MS.gene065349.t1
chr2.4 CDS 46834176 46834282 46834176 ID=cds.MS.gene065349.t1;Parent=MS.gene065349.t1
chr2.4 exon 46834315 46834606 46834315 ID=MS.gene065349.t1.exon2;Parent=MS.gene065349.t1
chr2.4 CDS 46834315 46834606 46834315 ID=cds.MS.gene065349.t1;Parent=MS.gene065349.t1
chr2.4 exon 46834751 46835010 46834751 ID=MS.gene065349.t1.exon3;Parent=MS.gene065349.t1
chr2.4 CDS 46834751 46835010 46834751 ID=cds.MS.gene065349.t1;Parent=MS.gene065349.t1
chr2.4 exon 46835049 46835147 46835049 ID=MS.gene065349.t1.exon4;Parent=MS.gene065349.t1
chr2.4 CDS 46835049 46835147 46835049 ID=cds.MS.gene065349.t1;Parent=MS.gene065349.t1
chr2.4 exon 46835200 46835312 46835200 ID=MS.gene065349.t1.exon5;Parent=MS.gene065349.t1
chr2.4 CDS 46835200 46835312 46835200 ID=cds.MS.gene065349.t1;Parent=MS.gene065349.t1
chr2.4 exon 46835973 46836498 46835973 ID=MS.gene065349.t1.exon6;Parent=MS.gene065349.t1
chr2.4 CDS 46835973 46836498 46835973 ID=cds.MS.gene065349.t1;Parent=MS.gene065349.t1
Gene Sequence

>MS.gene065349

CACCATGCCCAAATGGTTGGGTTATGGACCCTAATAAGACCAAGTGCTTTCTCCATGTTGGGAGGCCTCAATCATGGAATGACTCAGAGACATGTTGTAATAATATGGTGGGCATTTAGCATCATTCGCATCACTTCAGGAGTTACATTTTGCTCAAAGTCTGTGTGGTGAATCCATAAATAGTTGCTGGATTGGTGGAAGAAGACTCAACAACACTATGTCGGGTTTTCAGTGGACATGGTCTGATGATTCACAATGGAATAAGTCCATATTCCCTTTGGCTAATGTTCCACTTAATTGTACCGGAACCGGACAATTCTGTCTTAGGAACATCACAGAGTATTTATGTGCAGTAATGTCTAATAATTCAAAATCTCTCTTAAGTGAGAGATGTGATAATCCTCATGCCTCTCTATGTATACTTGACTTAGGTATGCTCTTGTTTATATTACCCACGGATTCTCATTTTACATTCATTAGAAATTCAGTATGTATATTTATCAATTATTTATTTAATTCATTCTTTCAAAAGGTTTATCTTTTTTATTTTATTTTTGGGTGTGCCATTTTTTCAGATACAAAATGTAATCATATGCACTGCCATAGGGAATACCTTATCATCCTTGCAGTGGTAAGTGGAGTGATACTCTTGACAACATTAGCAGTAGTTGTATGGCTTCTTGTATATAAGCAGGGCAAGAAGAGAAGACGATCTAGAAAACTAACTGATCCTGCTGCTACACTTCCATCATGGAAAATCTTTACAAAAGAGGAACTAAGGTCAATTACGAAAAATTTCAGTGAAGGAAACCGCCTTGTTGGAGATGCAAGACTGGTGGTACGTACAGTGGGGTCCAAGCTGATGGCTCAAAGGTTGCAGTTAAGAGATTAAAAAGGGCAAGCTTTAAGAGGAAAAAGGAGTTCTATTCTGAAATTGGCAGGGTTGCAAGGCTTCGACATCCAAACCTGGTGGTTTGTGATGGGATGCTGCTATGATCATGGTGACCGCTACATTGTTTATGAGTTTGTAGCTAATGGGCCCTTAGATAAATGGCTACATCACATACCAAGGGGAGGTCGCAGCTTAGATTGGGCTATGAGGATGAAAATTGCAACGACGCTTGCTCAAGGAATAGCGTAAGTTCAAAATATACTTATTCTTTGATAACTTTCTACAAGCTTTGTCTAATGTGCACAAGTAAATAAGTCTTGCACCATACGCAAGTTTGTTTTTTTTCCATTGGATTATAAATCACATCATATCACATTTGATCCAATGCTAAGACTTTCTGATTTAACGGTGGAAACAAGCTTGTGCATGATGCAAGACTTGTTCCACTTGTGTACCGTAGATTTGGCCCTTTCTACATGTCCATTTAGAATTATTCTCTCATAATTATACATGTTTTCACTCTATTAATAGAAGTATTCTTATGAAATCTAGCACCGTCTTATGTAATAAATATGTTGATAGCACATCTCTTATACAAATCTAGCACTGCCTTGTATGTTTTCAAGATTTTCATGAAGAAACTTGATACCCATTTCCAGGGCACCACATATGTTTGTTGGGTTTCACTTAGTTATGGATTATAGTTTCATGACTTCATCCCTTCAAACAAAAGTATTTCTTATAACTTGTTGAAATCTTTACGAGTCTTTTTTTTTTTTTTTTTTTGGTCAACCTTTACGAGTTTGATTTACTAACAAGAAAAAAGGATTATATAGCCCATAGCATTTTAAACTCGGTGGATATATTAATCACAATTTGTTTATTTTTTTCTCCCGTTTCAACAGGTTTCTGCATGACAAAGTTAAGCCACAAGTTGTTCATCGAGATATTCGAGCTAGTAATGTGCTGCTGGACGAGGAGTTTGGAGCACACCTTATGGGTGTTGGCTTGTCCAAGTTTGTGCCATATGAAGCGATGCATGAGAGGACTGTGATGGCAGGGGGAACTTATGGATATCTTGCTCCAGAGTTTGTGTACCGAAACGAGCTTACAACAAAGAGTGACGTTTATAGTTTTGGTGTCCTACTACTCGAAATAGTTAGTGGACGTAGACCAGCACAAGCAGTTGATTCTGTGGGTTGGCAGAGTATATTTGAATGGGCTACACCTCTTGTTCAAGCTCATCGCTACCCTGAACTCTTGGATCCTTATATATCTTCCTCATCTACTAGTATCATACCAGAGACTAGTACTATCCAGAAGGTGGTTGACCTTGTCTATTCATGCACGCAGCATGTCCCTTCTATGCGCCCTAGAATGTCACACGTTGTTCATCAGCTGCAACAGTTTACCCAGCCACCTTCAAAATAA
Protein sequence

>MS.gene065349.t1

HHAQMVGLWTLIRPSAFSMLGGLNHGMTQRHVVIIWSYILLKVCVVNPUIVAGLVEEDSTTLCRVFSGHGLMIHNGISPYSLWLMFHLIVPEPDNSVLGTSQSIYVQUCLIIQNLSUVRDVIILMPLYVYLTUIQNVIICTAIGNTLSSLQWUVEUYSUQHUQULYGFLYISRARREDDLENULILLLHFHHGKSLQKRNUGQLRKISVKETALLEMQDWLQLRDUKGQALRGKRSSILKLAGLQGFDIQTWCLULMGPUINGYITYQGEVAAUIGLUGUKLQRRLLKEURFCMTKLSHKLFIEIFELVMCCWTRSLEHTLWVLACPSLCHMKRCMRGLUWQGELMDILLQSLCTETSLQQRVTFIVLVSYYSKULVDVDQHKQLILWVGRVYLNGLHLLFKLIATLNSWILIYLPHLLVSYQRLVLSRRWLTLSIHARSMSLLCALECHTLFISCNSLPSHLQN