Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065917.t1 | XP_024638675.1 | 96.6 | 290 | 10 | 0 | 1 | 290 | 1 | 290 | 8.90E-147 | 529.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065917.t1 | O49460 | 83.9 | 286 | 46 | 0 | 4 | 289 | 2 | 287 | 1.2e-131 | 470.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065917.t1 | G7JSU6 | 96.6 | 290 | 10 | 0 | 1 | 290 | 4 | 293 | 6.4e-147 | 529.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene065917 | 0.806722 | 7.17E-50 | -1.69E-46 |
| MS.gene050791 | MS.gene065917 | 0.851756 | 7.21E-61 | -1.69E-46 |
| MS.gene050912 | MS.gene065917 | 0.836368 | 9.75E-57 | -1.69E-46 |
| MS.gene051984 | MS.gene065917 | 0.829182 | 5.94E-55 | -1.69E-46 |
| MS.gene056257 | MS.gene065917 | 0.807618 | 4.63E-50 | -1.69E-46 |
| MS.gene057272 | MS.gene065917 | 0.808057 | 3.73E-50 | -1.69E-46 |
| MS.gene060483 | MS.gene065917 | 0.80934 | 1.99E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065917.t1 | MTR_4g078200 | 96.552 | 290 | 10 | 0 | 1 | 290 | 4 | 293 | 0.0 | 569 |
| MS.gene065917.t1 | MTR_2g090760 | 86.063 | 287 | 40 | 0 | 3 | 289 | 1 | 287 | 0.0 | 501 |
| MS.gene065917.t1 | MTR_4g078200 | 96.970 | 231 | 7 | 0 | 60 | 290 | 2 | 232 | 1.83e-162 | 451 |
| MS.gene065917.t1 | MTR_8g046300 | 53.689 | 244 | 112 | 1 | 28 | 270 | 22 | 265 | 1.03e-89 | 268 |
| MS.gene065917.t1 | MTR_3g008250 | 50.943 | 265 | 128 | 2 | 17 | 279 | 10 | 274 | 3.87e-89 | 267 |
| MS.gene065917.t1 | MTR_5g093030 | 32.143 | 168 | 112 | 2 | 28 | 194 | 22 | 188 | 6.97e-25 | 99.0 |
| MS.gene065917.t1 | MTR_6g004320 | 34.483 | 145 | 57 | 1 | 98 | 242 | 1 | 107 | 4.69e-18 | 78.2 |
| MS.gene065917.t1 | MTR_5g093040 | 33.684 | 95 | 57 | 1 | 28 | 122 | 18 | 106 | 1.36e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065917.t1 | AT4G28510 | 83.624 | 287 | 47 | 0 | 3 | 289 | 1 | 287 | 5.34e-168 | 467 |
| MS.gene065917.t1 | AT1G03860 | 80.351 | 285 | 54 | 1 | 1 | 285 | 1 | 283 | 3.13e-167 | 465 |
| MS.gene065917.t1 | AT1G03860 | 80.351 | 285 | 54 | 1 | 1 | 285 | 1 | 283 | 3.13e-167 | 465 |
| MS.gene065917.t1 | AT2G20530 | 80.345 | 290 | 53 | 1 | 1 | 290 | 1 | 286 | 7.60e-167 | 464 |
| MS.gene065917.t1 | AT2G20530 | 80.345 | 290 | 53 | 1 | 1 | 290 | 1 | 286 | 7.60e-167 | 464 |
| MS.gene065917.t1 | AT5G44140 | 73.741 | 278 | 73 | 0 | 3 | 280 | 1 | 278 | 2.38e-151 | 424 |
| MS.gene065917.t1 | AT1G03860 | 85.780 | 218 | 31 | 0 | 68 | 285 | 1 | 218 | 1.29e-129 | 367 |
| MS.gene065917.t1 | AT3G27280 | 50.189 | 265 | 130 | 2 | 17 | 279 | 11 | 275 | 2.25e-89 | 267 |
| MS.gene065917.t1 | AT3G27280 | 50.189 | 265 | 130 | 2 | 17 | 279 | 11 | 275 | 2.25e-89 | 267 |
| MS.gene065917.t1 | AT5G40770 | 51.765 | 255 | 122 | 1 | 17 | 270 | 11 | 265 | 3.55e-88 | 264 |
| MS.gene065917.t1 | AT5G14300 | 45.339 | 236 | 102 | 2 | 36 | 270 | 22 | 231 | 2.31e-69 | 215 |
Find 72 sgRNAs with CRISPR-Local
Find 221 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAATCAATTGAGCACTTT+TGG | 0.231448 | 4.1:+27237567 | None:intergenic |
| CTTGGTGGACTTGCTGTCTA+TGG | 0.237252 | 4.1:-27240791 | MS.gene065917:CDS |
| TTGATTAAAGTTAGCATTCT+TGG | 0.252178 | 4.1:-27240809 | MS.gene065917:CDS |
| GAATCTCCAGGATTTAAAAT+TGG | 0.279612 | 4.1:-27237422 | MS.gene065917:CDS |
| TTCGATAGACCGTTGGTAAT+TGG | 0.291628 | 4.1:+27238520 | None:intergenic |
| CATTTAGTGGAGAGCACTTC+AGG | 0.305741 | 4.1:-27239508 | MS.gene065917:CDS |
| CATCCAGTGCAATGTTGAAT+TGG | 0.307846 | 4.1:+27238155 | None:intergenic |
| CCACCACCACCGGGAACCTT+TGG | 0.317038 | 4.1:+27240842 | None:intergenic |
| TCTATCACAAGTTTGACTTT+TGG | 0.348882 | 4.1:-27238127 | MS.gene065917:CDS |
| ATCATAATATGTGTTCCTTC+AGG | 0.372217 | 4.1:+27239577 | None:intergenic |
| TGCATTCAAGAAAACCTTGT+TGG | 0.399906 | 4.1:+27237459 | None:intergenic |
| GTCTATAAATGCAGGGTTGT+TGG | 0.403645 | 4.1:+27237534 | None:intergenic |
| CACCACCACCGGGAACCTTT+GGG | 0.411013 | 4.1:+27240843 | None:intergenic |
| GTCAAGATCCCAAAGGTTCC+CGG | 0.419171 | 4.1:-27240851 | MS.gene065917:CDS |
| TTCCTCAAGTCTATAAATGC+AGG | 0.423117 | 4.1:+27237526 | None:intergenic |
| ATTGTCTTTAACCGTGTTGT+TGG | 0.431088 | 4.1:-27240725 | MS.gene065917:CDS |
| ATCCCAAAGGTTCCCGGTGG+TGG | 0.434393 | 4.1:-27240845 | MS.gene065917:CDS |
| GCCAAAAGTGCTCAATTGAT+TGG | 0.439483 | 4.1:-27237568 | MS.gene065917:CDS |
| AGGCTGTTAGTCGAGAAATA+AGG | 0.442888 | 4.1:-27238201 | MS.gene065917:CDS |
| TTCTTGAGCAGCAACCTGCT+TGG | 0.447199 | 4.1:+27238078 | None:intergenic |
| TCGTGCACGACCTCATTTAG+TGG | 0.454933 | 4.1:-27239521 | MS.gene065917:CDS |
| AGCGTTTCATGTATAATAGA+AGG | 0.462263 | 4.1:+27238465 | None:intergenic |
| ATCCAGTGCAATGTTGAATT+GGG | 0.464199 | 4.1:+27238156 | None:intergenic |
| CCAAAGGTTCCCGGTGGTGG+TGG | 0.465351 | 4.1:-27240842 | MS.gene065917:CDS |
| AACACTTTGTATAATGTTGA+GGG | 0.467807 | 4.1:-27240761 | MS.gene065917:CDS |
| GGATCTCTTGCTGAATCTCC+AGG | 0.469133 | 4.1:-27237434 | MS.gene065917:CDS |
| TGTTGTTGGTGTCAAGGATA+AGG | 0.472048 | 4.1:-27240711 | MS.gene065917:intron |
| ATCACAGGCCTCTCAAACCA+TGG | 0.472479 | 4.1:+27239553 | None:intergenic |
| ACATAACTATTTCTTTCCAC+TGG | 0.472566 | 4.1:+27237396 | None:intergenic |
| AGCAGAAATTCCACCACCAC+CGG | 0.480850 | 4.1:+27240832 | None:intergenic |
| ACCAACGGTCTATCGAACTC+TGG | 0.482518 | 4.1:-27238514 | MS.gene065917:CDS |
| AGCTGCAATTGAAGCCAAGC+AGG | 0.488097 | 4.1:-27238092 | MS.gene065917:CDS |
| TAAGGAAGATATTGACTGAA+AGG | 0.495116 | 4.1:-27238183 | MS.gene065917:CDS |
| TAGACCGTTGGTAATTGGTC+TGG | 0.503898 | 4.1:+27238525 | None:intergenic |
| TAACCGTGTTGTTGGTGTCA+AGG | 0.506383 | 4.1:-27240717 | MS.gene065917:CDS |
| ATTAAAGTTAGCATTCTTGG+TGG | 0.509506 | 4.1:-27240806 | MS.gene065917:CDS |
| AAGGAAGATATTGACTGAAA+GGG | 0.510989 | 4.1:-27238182 | MS.gene065917:CDS |
| AAATTACCATTTGGAGATCA+CGG | 0.515095 | 4.1:+27239482 | None:intergenic |
| TGGGTGAGAATTATAATGAA+AGG | 0.516327 | 4.1:-27238494 | MS.gene065917:CDS |
| GAGGAAAATTGAAGCTGCTA+GGG | 0.523704 | 4.1:-27237509 | MS.gene065917:CDS |
| GGGTGAGAATTATAATGAAA+GGG | 0.533630 | 4.1:-27238493 | MS.gene065917:CDS |
| AAACACTTTGTATAATGTTG+AGG | 0.536366 | 4.1:-27240762 | MS.gene065917:CDS |
| ACCCTGCATTTATAGACTTG+AGG | 0.536732 | 4.1:-27237528 | MS.gene065917:CDS |
| AAGTGCTATTATTAGAGCAC+AGG | 0.538979 | 4.1:-27238008 | MS.gene065917:intron |
| GTTGGTAATTGGTCTGGCAC+GGG | 0.547210 | 4.1:+27238531 | None:intergenic |
| TCCTCAAGTCTATAAATGCA+GGG | 0.549653 | 4.1:+27237527 | None:intergenic |
| TGAGGAAAATTGAAGCTGCT+AGG | 0.555994 | 4.1:-27237510 | MS.gene065917:CDS |
| CGTTGGTAATTGGTCTGGCA+CGG | 0.557172 | 4.1:+27238530 | None:intergenic |
| CTCCCAATTCAACATTGCAC+TGG | 0.559449 | 4.1:-27238158 | MS.gene065917:CDS |
| TTATGATCCCATGGTTTGAG+AGG | 0.566462 | 4.1:-27239561 | MS.gene065917:CDS |
| AATTGCAAATTCTGCCAACA+AGG | 0.567716 | 4.1:-27237473 | MS.gene065917:CDS |
| AGGAAGCCGTGATCTCCAAA+TGG | 0.568285 | 4.1:-27239488 | MS.gene065917:intron |
| TATATGCAGGTCTATCCTGA+AGG | 0.571690 | 4.1:-27239592 | MS.gene065917:intron |
| CCAACGGTCTATCGAACTCT+GGG | 0.576164 | 4.1:-27238513 | MS.gene065917:CDS |
| GAAGTGCTCTCCACTAAATG+AGG | 0.579441 | 4.1:+27239511 | None:intergenic |
| AGCTGCTAGGGAAATCGCTA+AGG | 0.579747 | 4.1:-27237497 | MS.gene065917:CDS |
| GAACACATATTATGATCCCA+TGG | 0.591378 | 4.1:-27239570 | MS.gene065917:CDS |
| TTTCATGTATAATAGAAGGC+AGG | 0.592510 | 4.1:+27238469 | None:intergenic |
| GATTTAAAATTGGAGTCCAG+TGG | 0.610027 | 4.1:-27237412 | MS.gene065917:CDS |
| CCCAGAGTTCGATAGACCGT+TGG | 0.613107 | 4.1:+27238513 | None:intergenic |
| TCACAGGCCTCTCAAACCAT+GGG | 0.623389 | 4.1:+27239554 | None:intergenic |
| CAGTGCAATGTTGAATTGGG+AGG | 0.624755 | 4.1:+27238159 | None:intergenic |
| GCACGAACATCATAGATCAC+AGG | 0.624896 | 4.1:+27239538 | None:intergenic |
| GCAGAAATTCCACCACCACC+GGG | 0.625221 | 4.1:+27240833 | None:intergenic |
| ACATGAAACGCTCAAATCTG+TGG | 0.625780 | 4.1:-27238454 | MS.gene065917:CDS |
| TCAGCTCATTACTCAGAGAG+AGG | 0.636764 | 4.1:-27238412 | MS.gene065917:intron |
| TATCCTTGACACCAACAACA+CGG | 0.647254 | 4.1:+27240714 | None:intergenic |
| CAACAACGTCAAGATCCCAA+AGG | 0.651733 | 4.1:-27240858 | MS.gene065917:CDS |
| ACTTTGTATAATGTTGAGGG+TGG | 0.664841 | 4.1:-27240758 | MS.gene065917:CDS |
| TCTCTGAGTAATGAGCTGAC+TGG | 0.669211 | 4.1:+27238416 | None:intergenic |
| CGTGCCAGACCAATTACCAA+CGG | 0.684962 | 4.1:-27238529 | MS.gene065917:CDS |
| AAGATCCCAAAGGTTCCCGG+TGG | 0.741647 | 4.1:-27240848 | MS.gene065917:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAATGAATATGAATTA+TGG | + | chr4.1:27239464-27239483 | None:intergenic | 10.0% |
| !! | TAATAATGAATATGAATTAT+GGG | + | chr4.1:27239463-27239482 | None:intergenic | 10.0% |
| !!! | ATTTTTTAATAAGTCAATTT+TGG | + | chr4.1:27237869-27237888 | None:intergenic | 10.0% |
| !!! | TAATTTTATTTTGTATGATT+TGG | + | chr4.1:27240342-27240361 | None:intergenic | 10.0% |
| !!! | TTTTTTAATAAGTCAATTTT+GGG | + | chr4.1:27237868-27237887 | None:intergenic | 10.0% |
| !! | CAATAATATGATAAAAATGA+CGG | + | chr4.1:27238169-27238188 | None:intergenic | 15.0% |
| !! | TATATTGATATACTACAATA+AGG | + | chr4.1:27238645-27238664 | None:intergenic | 15.0% |
| !!! | TTTTTAATAAGTCAATTTTG+GGG | + | chr4.1:27237867-27237886 | None:intergenic | 15.0% |
| !! | AAAAAGCTTCTATACTAAAA+AGG | + | chr4.1:27238218-27238237 | None:intergenic | 20.0% |
| !! | AAACAAAAGAAATTACCATT+TGG | + | chr4.1:27238798-27238817 | None:intergenic | 20.0% |
| !! | AACATAAATTTCGAATTTCA+TGG | + | chr4.1:27239522-27239541 | None:intergenic | 20.0% |
| !! | AGTGTACATAGTAATAATTA+GGG | - | chr4.1:27239255-27239274 | MS.gene065917:intron | 20.0% |
| !! | CATAAACTGAACTAAAAAAT+AGG | - | chr4.1:27238421-27238440 | MS.gene065917:CDS | 20.0% |
| !! | CTAACGAAACATATTAAAAT+AGG | + | chr4.1:27238598-27238617 | None:intergenic | 20.0% |
| !! | TAGTGTACATAGTAATAATT+AGG | - | chr4.1:27239254-27239273 | MS.gene065917:intron | 20.0% |
| !! | TTTGAGAAAAAAGATCAATA+GGG | + | chr4.1:27240610-27240629 | None:intergenic | 20.0% |
| !!! | AACTAACAATTCAGTTTTTT+AGG | - | chr4.1:27238497-27238516 | MS.gene065917:CDS | 20.0% |
| !!! | AGAATATCGTTTTATATGAT+TGG | - | chr4.1:27237935-27237954 | MS.gene065917:intron | 20.0% |
| !!! | CATATTAAAATAGGTTGAAT+AGG | + | chr4.1:27238589-27238608 | None:intergenic | 20.0% |
| !!! | CTACTTTAATTTATAACGAT+GGG | + | chr4.1:27239216-27239235 | None:intergenic | 20.0% |
| !!! | CTATTTTAATATGTTTCGTT+AGG | - | chr4.1:27238596-27238615 | MS.gene065917:intron | 20.0% |
| !!! | GAATATCGTTTTATATGATT+GGG | - | chr4.1:27237936-27237955 | MS.gene065917:intron | 20.0% |
| !!! | TCTAAAATGATCCAAATTAT+AGG | - | chr4.1:27240047-27240066 | MS.gene065917:intron | 20.0% |
| !!! | TCTACTTTAATTTATAACGA+TGG | + | chr4.1:27239217-27239236 | None:intergenic | 20.0% |
| !!! | TTTAATCTTCTTCTTTGTTT+AGG | - | chr4.1:27238007-27238026 | MS.gene065917:intron | 20.0% |
| !!! | TTTAGTATAGAAGCTTTTTA+GGG | - | chr4.1:27238219-27238238 | MS.gene065917:intron | 20.0% |
| !!! | TTTTAGTATAGAAGCTTTTT+AGG | - | chr4.1:27238218-27238237 | MS.gene065917:intron | 20.0% |
| !!! | TTTTGAGAAAAAAGATCAAT+AGG | + | chr4.1:27240611-27240630 | None:intergenic | 20.0% |
| ! | AAAAAGTAACTCCTATGTAT+TGG | + | chr4.1:27240635-27240654 | None:intergenic | 25.0% |
| ! | AAAAGTAACTCCTATGTATT+GGG | + | chr4.1:27240634-27240653 | None:intergenic | 25.0% |
| ! | AAACACTTTGTATAATGTTG+AGG | - | chr4.1:27237506-27237525 | MS.gene065917:CDS | 25.0% |
| ! | AACACTTTGTATAATGTTGA+GGG | - | chr4.1:27237507-27237526 | MS.gene065917:CDS | 25.0% |
| ! | AAGATCCATAATAGATTACA+TGG | + | chr4.1:27239675-27239694 | None:intergenic | 25.0% |
| ! | AATGTGATACAATTGAGAAT+TGG | + | chr4.1:27239419-27239438 | None:intergenic | 25.0% |
| ! | ACACAGTAATAAAGTTAGAA+TGG | + | chr4.1:27239581-27239600 | None:intergenic | 25.0% |
| ! | ACTTTAGTACACTTGATTAT+AGG | - | chr4.1:27238857-27238876 | MS.gene065917:intron | 25.0% |
| ! | ATTGTGCATAAGAAAAATGT+GGG | + | chr4.1:27239620-27239639 | None:intergenic | 25.0% |
| ! | CAACATTAAAATGACTTCAA+TGG | + | chr4.1:27238951-27238970 | None:intergenic | 25.0% |
| ! | CAACTAATACTGGATTAATT+GGG | - | chr4.1:27239913-27239932 | MS.gene065917:intron | 25.0% |
| ! | CATTTCCATGTAATCTATTA+TGG | - | chr4.1:27239667-27239686 | MS.gene065917:intron | 25.0% |
| ! | CTGATTCTAAATTGAAAGTT+CGG | - | chr4.1:27238458-27238477 | MS.gene065917:CDS | 25.0% |
| ! | GTGTACATAGTAATAATTAG+GGG | - | chr4.1:27239256-27239275 | MS.gene065917:intron | 25.0% |
| ! | TAAAGTAGAATATGATGCTT+TGG | - | chr4.1:27239228-27239247 | MS.gene065917:intron | 25.0% |
| ! | TAACTAACAGTGAATGAAAA+TGG | + | chr4.1:27237588-27237607 | None:intergenic | 25.0% |
| ! | TAACTTTATTACTGTGTAGA+CGG | - | chr4.1:27239584-27239603 | MS.gene065917:CDS | 25.0% |
| ! | TAAGAACAAGAAAAAATAGC+AGG | + | chr4.1:27238823-27238842 | None:intergenic | 25.0% |
| ! | TAGTAAAAATGTAATGTCCT+TGG | + | chr4.1:27239164-27239183 | None:intergenic | 25.0% |
| ! | TATGTGTGTAAAAACCTTAA+AGG | + | chr4.1:27239119-27239138 | None:intergenic | 25.0% |
| ! | TATTGTGCATAAGAAAAATG+TGG | + | chr4.1:27239621-27239640 | None:intergenic | 25.0% |
| ! | TCAACTAATACTGGATTAAT+TGG | - | chr4.1:27239912-27239931 | MS.gene065917:intron | 25.0% |
| ! | TGTACATAGTAATAATTAGG+GGG | - | chr4.1:27239257-27239276 | MS.gene065917:intron | 25.0% |
| ! | TTCAACTTCTCATTACTTAA+TGG | - | chr4.1:27240653-27240672 | MS.gene065917:intron | 25.0% |
| ! | TTGAGAAAAAAGATCAATAG+GGG | + | chr4.1:27240609-27240628 | None:intergenic | 25.0% |
| ! | TTGATTAAAGTTAGCATTCT+TGG | - | chr4.1:27237459-27237478 | MS.gene065917:CDS | 25.0% |
| !!! | ATGGTTTATTTTTGACATAC+AGG | - | chr4.1:27240672-27240691 | MS.gene065917:intron | 25.0% |
| !!! | TGGTTTATTTTTGACATACA+GGG | - | chr4.1:27240673-27240692 | MS.gene065917:intron | 25.0% |
| AAAATGAATCACAACCATAC+AGG | - | chr4.1:27240010-27240029 | MS.gene065917:intron | 30.0% | |
| AAATGCAATAATGTTGTCCA+AGG | - | chr4.1:27239144-27239163 | MS.gene065917:intron | 30.0% | |
| AAATGCATCAATAGGTAACA+AGG | + | chr4.1:27239193-27239212 | None:intergenic | 30.0% | |
| AAATTACCATTTGGAGATCA+CGG | + | chr4.1:27238789-27238808 | None:intergenic | 30.0% | |
| ACTAAATTGAAAAGCGAGAA+TGG | - | chr4.1:27239300-27239319 | MS.gene065917:intron | 30.0% | |
| AGCGTTTCATGTATAATAGA+AGG | + | chr4.1:27239806-27239825 | None:intergenic | 30.0% | |
| ATAAGGTTACTAGTCAACAT+AGG | + | chr4.1:27238628-27238647 | None:intergenic | 30.0% | |
| ATCAAATAGGTTCATTCAAG+AGG | + | chr4.1:27240529-27240548 | None:intergenic | 30.0% | |
| ATCAAGTGTACTAAAGTACA+TGG | + | chr4.1:27238854-27238873 | None:intergenic | 30.0% | |
| ATCATAATATGTGTTCCTTC+AGG | + | chr4.1:27238694-27238713 | None:intergenic | 30.0% | |
| ATCTTCTGTTCAACTAATAC+TGG | - | chr4.1:27239903-27239922 | MS.gene065917:intron | 30.0% | |
| ATTAAAGTTAGCATTCTTGG+TGG | - | chr4.1:27237462-27237481 | MS.gene065917:CDS | 30.0% | |
| ATTCCAACTGATATGTAAAG+AGG | + | chr4.1:27239945-27239964 | None:intergenic | 30.0% | |
| ATTCTATCATCTCTACAGTA+GGG | + | chr4.1:27237920-27237939 | None:intergenic | 30.0% | |
| GATATAAACTCTGGATAATC+AGG | - | chr4.1:27240416-27240435 | MS.gene065917:intron | 30.0% | |
| GGGTGAGAATTATAATGAAA+GGG | - | chr4.1:27239775-27239794 | MS.gene065917:intron | 30.0% | |
| GTATTAGTTGAACAGAAGAT+AGG | + | chr4.1:27239903-27239922 | None:intergenic | 30.0% | |
| TATTCTATCATCTCTACAGT+AGG | + | chr4.1:27237921-27237940 | None:intergenic | 30.0% | |
| TCATGTTTCCATAACATAGA+CGG | + | chr4.1:27238110-27238129 | None:intergenic | 30.0% | |
| TGAAACTGTGATACAGATTA+TGG | + | chr4.1:27239649-27239668 | None:intergenic | 30.0% | |
| TGGGTGAGAATTATAATGAA+AGG | - | chr4.1:27239774-27239793 | MS.gene065917:intron | 30.0% | |
| TGTTTGAGTGATATAAACTC+TGG | - | chr4.1:27240407-27240426 | MS.gene065917:intron | 30.0% | |
| TTCAAAATGAGTGTCCTTTA+AGG | - | chr4.1:27239102-27239121 | MS.gene065917:intron | 30.0% | |
| TTGTGCATAAGAAAAATGTG+GGG | + | chr4.1:27239619-27239638 | None:intergenic | 30.0% | |
| TTTCATGTATAATAGAAGGC+AGG | + | chr4.1:27239802-27239821 | None:intergenic | 30.0% | |
| TTTCTCAAAACCCAATACAT+AGG | - | chr4.1:27240621-27240640 | MS.gene065917:intron | 30.0% | |
| ! | AAGGAAGATATTGACTGAAA+GGG | - | chr4.1:27240086-27240105 | MS.gene065917:intron | 30.0% |
| ! | GAATCTCCAGGATTTAAAAT+TGG | - | chr4.1:27240846-27240865 | MS.gene065917:CDS | 30.0% |
| ! | GATAATAGCAACTTGTCTTT+TGG | - | chr4.1:27238879-27238898 | MS.gene065917:intron | 30.0% |
| ! | TAAGGAAGATATTGACTGAA+AGG | - | chr4.1:27240085-27240104 | MS.gene065917:intron | 30.0% |
| ! | TAGTATGCCTTTTTATATGC+AGG | - | chr4.1:27238663-27238682 | MS.gene065917:intron | 30.0% |
| !! | AAACGACACTCATTTTGAAA+TGG | - | chr4.1:27239357-27239376 | MS.gene065917:intron | 30.0% |
| !! | AAAGGACACTCATTTTGAAA+CGG | + | chr4.1:27239101-27239120 | None:intergenic | 30.0% |
| !! | CTATCACAAGTTTGACTTTT+GGG | - | chr4.1:27240142-27240161 | MS.gene065917:intron | 30.0% |
| !! | TCTATCACAAGTTTGACTTT+TGG | - | chr4.1:27240141-27240160 | MS.gene065917:intron | 30.0% |
| !! | TTATTTTGCCAGGTGAAAAT+TGG | - | chr4.1:27239695-27239714 | MS.gene065917:intron | 30.0% |
| !! | TTCAATGGACTAAACTGAAA+GGG | + | chr4.1:27238936-27238955 | None:intergenic | 30.0% |
| !! | TTTGTTTTCAGTCTCATGAT+CGG | - | chr4.1:27238068-27238087 | MS.gene065917:CDS | 30.0% |
| !!! | TATGGATCTTTTATTTTGCC+AGG | - | chr4.1:27239685-27239704 | MS.gene065917:intron | 30.0% |
| AAAAAACTTCTGCTCTGGTT+GGG | + | chr4.1:27238143-27238162 | None:intergenic | 35.0% | |
| AAATGAAAAGATCGCTGCAA+TGG | - | chr4.1:27240368-27240387 | MS.gene065917:intron | 35.0% | |
| AATTGCAAATTCTGCCAACA+AGG | - | chr4.1:27240795-27240814 | MS.gene065917:CDS | 35.0% | |
| ACACATCTTCAATAAGTGTC+AGG | - | chr4.1:27237655-27237674 | MS.gene065917:intron | 35.0% | |
| ACACTTATTGAAGATGTGTC+CGG | + | chr4.1:27237654-27237673 | None:intergenic | 35.0% | |
| ACATCTTCAATAAGTGTCAG+GGG | - | chr4.1:27237657-27237676 | MS.gene065917:intron | 35.0% | |
| ACTTGAACTCTATCCAAGTT+TGG | + | chr4.1:27238382-27238401 | None:intergenic | 35.0% | |
| ACTTTGTATAATGTTGAGGG+TGG | - | chr4.1:27237510-27237529 | MS.gene065917:CDS | 35.0% | |
| AGATGAAGTGACAGTTGTAT+TGG | + | chr4.1:27238333-27238352 | None:intergenic | 35.0% | |
| ATAGATGGCGTGGATTTATA+TGG | + | chr4.1:27239000-27239019 | None:intergenic | 35.0% | |
| ATATGATACATAAGCCCACA+AGG | + | chr4.1:27239035-27239054 | None:intergenic | 35.0% | |
| ATCCAGTGCAATGTTGAATT+GGG | + | chr4.1:27240115-27240134 | None:intergenic | 35.0% | |
| ATGCCTCTTTACATATCAGT+TGG | - | chr4.1:27239939-27239958 | MS.gene065917:intron | 35.0% | |
| ATTGTCTTTAACCGTGTTGT+TGG | - | chr4.1:27237543-27237562 | MS.gene065917:CDS | 35.0% | |
| ATTTCATGCCGTCTATGTTA+TGG | - | chr4.1:27238099-27238118 | MS.gene065917:CDS | 35.0% | |
| CACATCTTCAATAAGTGTCA+GGG | - | chr4.1:27237656-27237675 | MS.gene065917:intron | 35.0% | |
| GAACACATATTATGATCCCA+TGG | - | chr4.1:27238698-27238717 | MS.gene065917:intron | 35.0% | |
| GGATAGACCTGCATATAAAA+AGG | + | chr4.1:27238673-27238692 | None:intergenic | 35.0% | |
| GTCAAAAACTGAACCAAACT+TGG | - | chr4.1:27238366-27238385 | MS.gene065917:intron | 35.0% | |
| GTGATACAATTGAGAATTGG+TGG | + | chr4.1:27239416-27239435 | None:intergenic | 35.0% | |
| GTTCATTCAAGAGGAAAAAG+TGG | + | chr4.1:27240520-27240539 | None:intergenic | 35.0% | |
| TATGATTGGGTCATGTATGA+AGG | - | chr4.1:27237949-27237968 | MS.gene065917:intron | 35.0% | |
| TCCTCAAGTCTATAAATGCA+GGG | + | chr4.1:27240744-27240763 | None:intergenic | 35.0% | |
| TCGACTAACAGCCTATAATT+TGG | + | chr4.1:27240061-27240080 | None:intergenic | 35.0% | |
| TGCATTCAAGAAAACCTTGT+TGG | + | chr4.1:27240812-27240831 | None:intergenic | 35.0% | |
| TTCCTCAAGTCTATAAATGC+AGG | + | chr4.1:27240745-27240764 | None:intergenic | 35.0% | |
| ! | AAGTGCTATTATTAGAGCAC+AGG | - | chr4.1:27240260-27240279 | MS.gene065917:intron | 35.0% |
| ! | ACCAATCAATTGAGCACTTT+TGG | + | chr4.1:27240704-27240723 | None:intergenic | 35.0% |
| ! | CTTCTTTGTTTAGGTTCTCA+TGG | - | chr4.1:27238016-27238035 | MS.gene065917:CDS | 35.0% |
| ! | GAATGAACCTATTTGATGAC+TGG | - | chr4.1:27240532-27240551 | MS.gene065917:intron | 35.0% |
| ! | GATTTAAAATTGGAGTCCAG+TGG | - | chr4.1:27240856-27240875 | MS.gene065917:CDS | 35.0% |
| ! | GCTAATTTAAAGTGAGTTGC+AGG | - | chr4.1:27237720-27237739 | MS.gene065917:intron | 35.0% |
| ! | TGGACTCCAATTTTAAATCC+TGG | + | chr4.1:27240855-27240874 | None:intergenic | 35.0% |
| !! | CTTCAATGGACTAAACTGAA+AGG | + | chr4.1:27238937-27238956 | None:intergenic | 35.0% |
| !! | TAATAATTAGGGGGTACTGT+TGG | - | chr4.1:27239266-27239285 | MS.gene065917:intron | 35.0% |
| !!! | AAGGACACTCATTTTGAAAC+GGG | + | chr4.1:27239100-27239119 | None:intergenic | 35.0% |
| !!! | CAGTTTTTTAGGTTCAGTTC+TGG | - | chr4.1:27238508-27238527 | MS.gene065917:CDS | 35.0% |
| !!! | CTTGTCTTTTGGTTTGAAGT+TGG | - | chr4.1:27238890-27238909 | MS.gene065917:intron | 35.0% |
| !!! | GACACTCATTTTGAAATGGA+GGG | - | chr4.1:27239361-27239380 | MS.gene065917:intron | 35.0% |
| AAACTGAAAGGGTAAGGTGA+GGG | + | chr4.1:27238925-27238944 | None:intergenic | 40.0% | |
| AATAATCAGTCTCGCCTGTA+TGG | + | chr4.1:27240027-27240046 | None:intergenic | 40.0% | |
| ACATGAAACGCTCAAATCTG+TGG | - | chr4.1:27239814-27239833 | MS.gene065917:intron | 40.0% | |
| ACCCTGCATTTATAGACTTG+AGG | - | chr4.1:27240740-27240759 | MS.gene065917:CDS | 40.0% | |
| ACGATGGGAAATGCATCAAT+AGG | + | chr4.1:27239201-27239220 | None:intergenic | 40.0% | |
| AGGCTGTTAGTCGAGAAATA+AGG | - | chr4.1:27240067-27240086 | MS.gene065917:intron | 40.0% | |
| ATAAATCCACGCCATCTATG+TGG | - | chr4.1:27239001-27239020 | MS.gene065917:intron | 40.0% | |
| ATCAATAGGGGTCATGTTGA+AGG | + | chr4.1:27240597-27240616 | None:intergenic | 40.0% | |
| CAAAAAACTTCTGCTCTGGT+TGG | + | chr4.1:27238144-27238163 | None:intergenic | 40.0% | |
| CATCCAGTGCAATGTTGAAT+TGG | + | chr4.1:27240116-27240135 | None:intergenic | 40.0% | |
| CATCTTCAATAAGTGTCAGG+GGG | - | chr4.1:27237658-27237677 | MS.gene065917:intron | 40.0% | |
| CATGGATAAGTTGTCAAGGA+AGG | - | chr4.1:27240560-27240579 | MS.gene065917:intron | 40.0% | |
| GAGGAAAATTGAAGCTGCTA+GGG | - | chr4.1:27240759-27240778 | MS.gene065917:CDS | 40.0% | |
| GATGCATGGATAAGTTGTCA+AGG | - | chr4.1:27240556-27240575 | MS.gene065917:intron | 40.0% | |
| GGTTCAAAAAACTTCTGCTC+TGG | + | chr4.1:27238148-27238167 | None:intergenic | 40.0% | |
| GTCTATAAATGCAGGGTTGT+TGG | + | chr4.1:27240737-27240756 | None:intergenic | 40.0% | |
| TAAACTGAAAGGGTAAGGTG+AGG | + | chr4.1:27238926-27238945 | None:intergenic | 40.0% | |
| TATATGCAGGTCTATCCTGA+AGG | - | chr4.1:27238676-27238695 | MS.gene065917:intron | 40.0% | |
| TATCCTTGACACCAACAACA+CGG | + | chr4.1:27237557-27237576 | None:intergenic | 40.0% | |
| TGAGGAAAATTGAAGCTGCT+AGG | - | chr4.1:27240758-27240777 | MS.gene065917:CDS | 40.0% | |
| TGCATCACCAGTCATCAAAT+AGG | + | chr4.1:27240542-27240561 | None:intergenic | 40.0% | |
| TGGACTAAACTGAAAGGGTA+AGG | + | chr4.1:27238931-27238950 | None:intergenic | 40.0% | |
| TTATGATCCCATGGTTTGAG+AGG | - | chr4.1:27238707-27238726 | MS.gene065917:intron | 40.0% | |
| TTCAAGAGGAAAAAGTGGAG+AGG | + | chr4.1:27240515-27240534 | None:intergenic | 40.0% | |
| ! | ATTTGATGACTGGTGATGCA+TGG | - | chr4.1:27240542-27240561 | MS.gene065917:intron | 40.0% |
| ! | CAAGGTTTTCTTGAATGCAG+AGG | - | chr4.1:27240813-27240832 | MS.gene065917:CDS | 40.0% |
| ! | CTCTAAGTCCAATTTTCACC+TGG | + | chr4.1:27239706-27239725 | None:intergenic | 40.0% |
| !! | CACAAGTTTGACTTTTGGGA+GGG | - | chr4.1:27240146-27240165 | MS.gene065917:intron | 40.0% |
| !! | GCCAAAAGTGCTCAATTGAT+TGG | - | chr4.1:27240700-27240719 | MS.gene065917:intron | 40.0% |
| !! | TCACAAGTTTGACTTTTGGG+AGG | - | chr4.1:27240145-27240164 | MS.gene065917:intron | 40.0% |
| !! | TGTTGTTGGTGTCAAGGATA+AGG | - | chr4.1:27237557-27237576 | MS.gene065917:CDS | 40.0% |
| !! | TTCGATAGACCGTTGGTAAT+TGG | + | chr4.1:27239751-27239770 | None:intergenic | 40.0% |
| !!! | AGGACACTCATTTTGAAACG+GGG | + | chr4.1:27239099-27239118 | None:intergenic | 40.0% |
| !!! | CGACACTCATTTTGAAATGG+AGG | - | chr4.1:27239360-27239379 | MS.gene065917:intron | 40.0% |
| ACTCCAAAGACCTGCATACT+TGG | - | chr4.1:27238305-27238324 | MS.gene065917:intron | 45.0% | |
| AGAGGAAAAAGTGGAGAGGA+AGG | + | chr4.1:27240511-27240530 | None:intergenic | 45.0% | |
| AGGTCAACAGTCCACATAGA+TGG | + | chr4.1:27239015-27239034 | None:intergenic | 45.0% | |
| ATCGCTGCAATGGTATTTGC+TGG | - | chr4.1:27240378-27240397 | MS.gene065917:intron | 45.0% | |
| CAACAACGTCAAGATCCCAA+AGG | - | chr4.1:27237410-27237429 | MS.gene065917:CDS | 45.0% | |
| CAGTGCAATGTTGAATTGGG+AGG | + | chr4.1:27240112-27240131 | None:intergenic | 45.0% | |
| CTCCAAAGACCTGCATACTT+GGG | - | chr4.1:27238306-27238325 | MS.gene065917:intron | 45.0% | |
| CTCCCAATTCAACATTGCAC+TGG | - | chr4.1:27240110-27240129 | MS.gene065917:intron | 45.0% | |
| GAGGAAAAAGTGGAGAGGAA+GGG | + | chr4.1:27240510-27240529 | None:intergenic | 45.0% | |
| GCACGAACATCATAGATCAC+AGG | + | chr4.1:27238733-27238752 | None:intergenic | 45.0% | |
| TCAGCTCATTACTCAGAGAG+AGG | - | chr4.1:27239856-27239875 | MS.gene065917:intron | 45.0% | |
| TCTCTGAGTAATGAGCTGAC+TGG | + | chr4.1:27239855-27239874 | None:intergenic | 45.0% | |
| TGTATTGGACCCAAGTATGC+AGG | + | chr4.1:27238318-27238337 | None:intergenic | 45.0% | |
| ! | ACGGGGGGAGTATATGAAAT+AGG | + | chr4.1:27239082-27239101 | None:intergenic | 45.0% |
| ! | ATGTGGACTGTTGACCTTGT+GGG | - | chr4.1:27239018-27239037 | MS.gene065917:intron | 45.0% |
| ! | GAAGTGCTCTCCACTAAATG+AGG | + | chr4.1:27238760-27238779 | None:intergenic | 45.0% |
| ! | GAGAATTGGTGGTTCAGTTC+AGG | + | chr4.1:27239405-27239424 | None:intergenic | 45.0% |
| ! | TATGTGGACTGTTGACCTTG+TGG | - | chr4.1:27239017-27239036 | MS.gene065917:intron | 45.0% |
| !! | CATTTAGTGGAGAGCACTTC+AGG | - | chr4.1:27238760-27238779 | MS.gene065917:intron | 45.0% |
| !! | TAACCGTGTTGTTGGTGTCA+AGG | - | chr4.1:27237551-27237570 | MS.gene065917:CDS | 45.0% |
| !! | TAGACCGTTGGTAATTGGTC+TGG | + | chr4.1:27239746-27239765 | None:intergenic | 45.0% |
| !!! | GACACTCATTTTGAAACGGG+GGG | + | chr4.1:27239097-27239116 | None:intergenic | 45.0% |
| !!! | GGACACTCATTTTGAAACGG+GGG | + | chr4.1:27239098-27239117 | None:intergenic | 45.0% |
| !!! | TATATTTTTTTAAAAAACAA+CGG | + | chr4.1:27240309-27240328 | None:intergenic | 5.0% |
| AACAGTCCACATAGATGGCG+TGG | + | chr4.1:27239010-27239029 | None:intergenic | 50.0% | |
| ACCAACGGTCTATCGAACTC+TGG | - | chr4.1:27239754-27239773 | MS.gene065917:intron | 50.0% | |
| AGCAGAAATTCCACCACCAC+CGG | + | chr4.1:27237439-27237458 | None:intergenic | 50.0% | |
| AGCTGCAATTGAAGCCAAGC+AGG | - | chr4.1:27240176-27240195 | MS.gene065917:intron | 50.0% | |
| AGCTGCTAGGGAAATCGCTA+AGG | - | chr4.1:27240771-27240790 | MS.gene065917:CDS | 50.0% | |
| AGGAAGCCGTGATCTCCAAA+TGG | - | chr4.1:27238780-27238799 | MS.gene065917:intron | 50.0% | |
| ATCACAGGCCTCTCAAACCA+TGG | + | chr4.1:27238718-27238737 | None:intergenic | 50.0% | |
| CACGCATCAGTATCAGACAC+CGG | - | chr4.1:27237632-27237651 | MS.gene065917:intron | 50.0% | |
| CCAACGGTCTATCGAACTCT+GGG | - | chr4.1:27239755-27239774 | MS.gene065917:intron | 50.0% | |
| CGTGCCAGACCAATTACCAA+CGG | - | chr4.1:27239739-27239758 | MS.gene065917:intron | 50.0% | |
| GACCCAAGTATGCAGGTCTT+TGG | + | chr4.1:27238311-27238330 | None:intergenic | 50.0% | |
| GGATCTCTTGCTGAATCTCC+AGG | - | chr4.1:27240834-27240853 | MS.gene065917:CDS | 50.0% | |
| GTCAAGATCCCAAAGGTTCC+CGG | - | chr4.1:27237417-27237436 | MS.gene065917:CDS | 50.0% | |
| TCACAGGCCTCTCAAACCAT+GGG | + | chr4.1:27238717-27238736 | None:intergenic | 50.0% | |
| TCGTGCACGACCTCATTTAG+TGG | - | chr4.1:27238747-27238766 | MS.gene065917:intron | 50.0% | |
| TGAAAGGGTAAGGTGAGGGA+AGG | + | chr4.1:27238921-27238940 | None:intergenic | 50.0% | |
| TTCTTGAGCAGCAACCTGCT+TGG | + | chr4.1:27240193-27240212 | None:intergenic | 50.0% | |
| !! | CGTTGGTAATTGGTCTGGCA+CGG | + | chr4.1:27239741-27239760 | None:intergenic | 50.0% |
| !! | CTTGGTGGACTTGCTGTCTA+TGG | - | chr4.1:27237477-27237496 | MS.gene065917:CDS | 50.0% |
| !! | GTTGGTAATTGGTCTGGCAC+GGG | + | chr4.1:27239740-27239759 | None:intergenic | 50.0% |
| AAAGTGGAGAGGAAGGGAGC+AGG | + | chr4.1:27240504-27240523 | None:intergenic | 55.0% | |
| AAGATCCCAAAGGTTCCCGG+TGG | - | chr4.1:27237420-27237439 | MS.gene065917:CDS | 55.0% | |
| AAGTGGAGAGGAAGGGAGCA+GGG | + | chr4.1:27240503-27240522 | None:intergenic | 55.0% | |
| CCCAGAGTTCGATAGACCGT+TGG | + | chr4.1:27239758-27239777 | None:intergenic | 55.0% | |
| GCAGAAATTCCACCACCACC+GGG | + | chr4.1:27237438-27237457 | None:intergenic | 55.0% | |
| ATCCCAAAGGTTCCCGGTGG+TGG | - | chr4.1:27237423-27237442 | MS.gene065917:CDS | 60.0% | |
| CACCACCACCGGGAACCTTT+GGG | + | chr4.1:27237428-27237447 | None:intergenic | 60.0% | |
| CCACCACCACCGGGAACCTT+TGG | + | chr4.1:27237429-27237448 | None:intergenic | 65.0% | |
| ! | CCAAAGGTTCCCGGTGGTGG+TGG | - | chr4.1:27237426-27237445 | MS.gene065917:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 27237402 | 27240888 | 27237402 | ID=MS.gene065917 |
| chr4.1 | mRNA | 27237402 | 27240888 | 27237402 | ID=MS.gene065917.t1;Parent=MS.gene065917 |
| chr4.1 | exon | 27240712 | 27240888 | 27240712 | ID=MS.gene065917.t1.exon1;Parent=MS.gene065917.t1 |
| chr4.1 | CDS | 27240712 | 27240888 | 27240712 | ID=cds.MS.gene065917.t1;Parent=MS.gene065917.t1 |
| chr4.1 | exon | 27239489 | 27239605 | 27239489 | ID=MS.gene065917.t1.exon2;Parent=MS.gene065917.t1 |
| chr4.1 | CDS | 27239489 | 27239605 | 27239489 | ID=cds.MS.gene065917.t1;Parent=MS.gene065917.t1 |
| chr4.1 | exon | 27238413 | 27238583 | 27238413 | ID=MS.gene065917.t1.exon3;Parent=MS.gene065917.t1 |
| chr4.1 | CDS | 27238413 | 27238583 | 27238413 | ID=cds.MS.gene065917.t1;Parent=MS.gene065917.t1 |
| chr4.1 | exon | 27238009 | 27238221 | 27238009 | ID=MS.gene065917.t1.exon4;Parent=MS.gene065917.t1 |
| chr4.1 | CDS | 27238009 | 27238221 | 27238009 | ID=cds.MS.gene065917.t1;Parent=MS.gene065917.t1 |
| chr4.1 | exon | 27237402 | 27237596 | 27237402 | ID=MS.gene065917.t1.exon5;Parent=MS.gene065917.t1 |
| chr4.1 | CDS | 27237402 | 27237596 | 27237402 | ID=cds.MS.gene065917.t1;Parent=MS.gene065917.t1 |
| Gene Sequence |
| Protein sequence |
