AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050912


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050912.t1 MTR_7g112770 98.387 124 2 0 1 124 1 124 5.42e-87 249
MS.gene050912.t1 MTR_1g088450 94.915 118 6 0 7 124 2 119 8.92e-69 202
MS.gene050912.t1 MTR_1g083430 90.400 125 11 1 1 124 1 125 3.21e-68 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050912.t1 AT3G05560 88.710 124 14 0 1 124 1 124 3.08e-68 201
MS.gene050912.t1 AT3G05560 88.710 124 14 0 1 124 1 124 3.08e-68 201
MS.gene050912.t1 AT3G05560 88.710 124 14 0 1 124 1 124 3.08e-68 201
MS.gene050912.t1 AT5G27770 87.611 113 14 0 1 113 1 113 9.50e-53 162
MS.gene050912.t1 AT1G02830 71.818 110 31 0 6 115 8 117 1.06e-44 142

Find 26 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCACCCTTCTTCTTTCCCT+TGG 0.344547 7.1:-3369228 None:intergenic
TCCATGATCTTGTCTTCCAC+TGG 0.448631 7.1:-3369275 None:intergenic
GGTGGAAAAGCTGGTGCTCT+TGG 0.478615 7.1:+3369341 MS.gene050912:CDS
ATCAAGGTCGGTGGAAAAGC+TGG 0.491281 7.1:+3369332 MS.gene050912:CDS
ATGAGTCGTGGTAACGCAGC+TGG 0.519123 7.1:+3369203 MS.gene050912:CDS
CACTGGCTTAGCACAGTCAA+TGG 0.524581 7.1:-3369258 None:intergenic
CATTGCTGAGAACGAGGGAG+AGG 0.527747 7.1:+3370625 MS.gene050912:CDS
TGGTGCCAAGGGAAAGAAGA+AGG 0.529349 7.1:+3369223 MS.gene050912:CDS
CTTGCTCTTCTCACGGGTAA+CGG 0.534877 7.1:-3369375 None:intergenic
TGGTAACGCAGCTGGTGCCA+AGG 0.535573 7.1:+3369211 MS.gene050912:CDS
GTTTCTTCAAGAGCGAATCA+AGG 0.539769 7.1:+3369316 MS.gene050912:CDS
ATAGAAGTATCTATGAGCTC+AGG 0.546088 7.1:+3370594 MS.gene050912:CDS
AGTGATCTTGCTCTTCTCAC+GGG 0.552636 7.1:-3369381 None:intergenic
CAGTGATCTTGCTCTTCTCA+CGG 0.553223 7.1:-3369382 None:intergenic
GCCAGTGGAAGACAAGATCA+TGG 0.553292 7.1:+3369274 MS.gene050912:CDS
CTTCTCACGGGTAACGGTGA+CGG 0.568235 7.1:-3369369 None:intergenic
AGAAACACAATGTAAGAGAC+TGG 0.568481 7.1:+3370546 MS.gene050912:CDS
GGTAACGCAGCTGGTGCCAA+GGG 0.575575 7.1:+3369212 MS.gene050912:CDS
CTTCAACATTGCTGAGAACG+AGG 0.581013 7.1:+3370619 MS.gene050912:CDS
CTTGGAAAAGTTACTGTCAG+AGG 0.604590 7.1:-3369408 None:intergenic
CTTAGCACAGTCAATGGTGA+AGG 0.610280 7.1:-3369252 None:intergenic
CTTCAAGAGCGAATCAAGGT+CGG 0.612896 7.1:+3369320 MS.gene050912:CDS
CATTGACTGTGCTAAGCCAG+TGG 0.621872 7.1:+3369259 MS.gene050912:CDS
CAAGAGCGAATCAAGGTCGG+TGG 0.643278 7.1:+3369323 MS.gene050912:CDS
GGTGCCAAGGGAAAGAAGAA+GGG 0.657143 7.1:+3369224 MS.gene050912:CDS
TTCAACATTGCTGAGAACGA+GGG 0.691023 7.1:+3370620 MS.gene050912:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGATAAATGATAGTTAT+GGG - chr7.1:3370168-3370187 None:intergenic 15.0%
!!! GCTTTGATTTAATATTTTAT+TGG + chr7.1:3369487-3369506 MS.gene050912:intron 15.0%
!! AAAGATAAATGATAGAGTAA+TGG - chr7.1:3370113-3370132 None:intergenic 20.0%
!! AAGATAAATGATAGAGTAAT+GGG - chr7.1:3370112-3370131 None:intergenic 20.0%
!! ACATCTAATTAATAGCTTAT+TGG + chr7.1:3369843-3369862 MS.gene050912:intron 20.0%
!! ATACTATATTCTGTAGATAT+AGG - chr7.1:3370293-3370312 None:intergenic 20.0%
!! ATATCTACAGAATATAGTAT+TGG + chr7.1:3370293-3370312 MS.gene050912:intron 20.0%
!! GTAAAGATAAATGATAGTTA+TGG - chr7.1:3370169-3370188 None:intergenic 20.0%
!! TATCTACAGAATATAGTATT+GGG + chr7.1:3370294-3370313 MS.gene050912:intron 20.0%
!!! TAATAAGTGTTCTTTAAGAT+AGG + chr7.1:3369766-3369785 MS.gene050912:intron 20.0%
! AAGTAGTCTCACAAAATTTA+TGG + chr7.1:3370051-3370070 MS.gene050912:intron 25.0%
! AATTAGATGTTTATCCAAAC+AGG - chr7.1:3369833-3369852 None:intergenic 25.0%
! ATAACTTAGTGCTTATACAA+CGG + chr7.1:3369797-3369816 MS.gene050912:intron 25.0%
! ATTCAGTGAAGATAAGTTAT+TGG + chr7.1:3369534-3369553 MS.gene050912:intron 25.0%
!! ATAAGTTTTGCGAAACTATT+TGG + chr7.1:3369682-3369701 MS.gene050912:intron 25.0%
!! TAAGTTTTGCGAAACTATTT+GGG + chr7.1:3369683-3369702 MS.gene050912:intron 25.0%
!! TTGCTTGGTTGATTTATTTA+TGG + chr7.1:3369574-3369593 MS.gene050912:intron 25.0%
!!! AAGATAGTTGATAGTCATTT+TGG + chr7.1:3369608-3369627 MS.gene050912:intron 25.0%
!!! TCATTTTGGTTTCAATTTGT+TGG + chr7.1:3369622-3369641 MS.gene050912:intron 25.0%
AAGTTATTGGCATGAAATCA+TGG + chr7.1:3369547-3369566 MS.gene050912:intron 30.0%
AGACCTTATGTTATTAGTTG+CGG - chr7.1:3370467-3370486 None:intergenic 30.0%
AGTACGATAACAACAGTATA+AGG + chr7.1:3370382-3370401 MS.gene050912:intron 30.0%
AGTTATGGGACTGTTAAAAT+TGG - chr7.1:3370154-3370173 None:intergenic 30.0%
CAGTATAAGGTCTACTTAAT+TGG + chr7.1:3370395-3370414 MS.gene050912:intron 30.0%
GTAAATGAATTATTACCGCT+TGG - chr7.1:3369429-3369448 None:intergenic 30.0%
TAGTCTCACAAAATTTATGG+CGG + chr7.1:3370054-3370073 MS.gene050912:intron 30.0%
TCAGCAAACTAATTCATTGA+AGG + chr7.1:3370320-3370339 MS.gene050912:intron 30.0%
TTTCCGCAACTAATAACATA+AGG + chr7.1:3370461-3370480 MS.gene050912:intron 30.0%
!! GTGTTCTTTAAGATAGGTTA+TGG + chr7.1:3369772-3369791 MS.gene050912:intron 30.0%
!! TTTCAATTTGTTGGTCTGTT+TGG + chr7.1:3369631-3369650 MS.gene050912:intron 30.0%
!!! AGCTGATATTTTGCTGTTTT+TGG + chr7.1:3370423-3370442 MS.gene050912:intron 30.0%
!!! GCTGATATTTTGCTGTTTTT+GGG + chr7.1:3370424-3370443 MS.gene050912:intron 30.0%
AACTTACGAACAAGAGCTTA+TGG + chr7.1:3369708-3369727 MS.gene050912:intron 35.0%
AGAAACACAATGTAAGAGAC+TGG + chr7.1:3370546-3370565 MS.gene050912:CDS 35.0%
GCTGTCTGAATAAGCTATAT+TGG + chr7.1:3369971-3369990 MS.gene050912:intron 35.0%
GCTTATTTGAGCTTGTCTAT+GGG + chr7.1:3369658-3369677 MS.gene050912:intron 35.0%
TGAAATCATGGTTCATTGCT+TGG + chr7.1:3369559-3369578 MS.gene050912:intron 35.0%
TGCTTATACAACGGCAATAT+AGG + chr7.1:3369806-3369825 MS.gene050912:intron 35.0%
! AGTAATGGGACTGTTAAGAT+TGG - chr7.1:3370098-3370117 None:intergenic 35.0%
! ATTTGTTGGTCTGTTTGGAT+TGG + chr7.1:3369636-3369655 MS.gene050912:intron 35.0%
! CAAATCATTGTTTGCTACTC+TGG + chr7.1:3369448-3369467 MS.gene050912:intron 35.0%
!! ATAGAAGTATCTATGAGCTC+AGG + chr7.1:3370594-3370613 MS.gene050912:CDS 35.0%
ATAAGCTGTCTATCCCAACA+CGG + chr7.1:3370267-3370286 MS.gene050912:intron 40.0%
CTTGGAAAAGTTACTGTCAG+AGG - chr7.1:3369411-3369430 None:intergenic 40.0%
GGCTTATTTGAGCTTGTCTA+TGG + chr7.1:3369657-3369676 MS.gene050912:intron 40.0%
GTTTCTTCAAGAGCGAATCA+AGG + chr7.1:3369316-3369335 MS.gene050912:CDS 40.0%
TTCAACATTGCTGAGAACGA+GGG + chr7.1:3370620-3370639 MS.gene050912:CDS 40.0%
! CTGACAGTAACTTTTCCAAG+CGG + chr7.1:3369411-3369430 MS.gene050912:CDS 40.0%
ACGGCAATATAGGTCCTGTT+TGG + chr7.1:3369816-3369835 MS.gene050912:intron 45.0%
AGTGATCTTGCTCTTCTCAC+GGG - chr7.1:3369384-3369403 None:intergenic 45.0%
CAGTGATCTTGCTCTTCTCA+CGG - chr7.1:3369385-3369404 None:intergenic 45.0%
CTGTAGATATAGGCCGTGTT+GGG - chr7.1:3370283-3370302 None:intergenic 45.0%
CTTAGCACAGTCAATGGTGA+AGG - chr7.1:3369255-3369274 None:intergenic 45.0%
CTTCAACATTGCTGAGAACG+AGG + chr7.1:3370619-3370638 MS.gene050912:CDS 45.0%
CTTCAAGAGCGAATCAAGGT+CGG + chr7.1:3369320-3369339 MS.gene050912:CDS 45.0%
TCCATGATCTTGTCTTCCAC+TGG - chr7.1:3369278-3369297 None:intergenic 45.0%
TCTGTAGATATAGGCCGTGT+TGG - chr7.1:3370284-3370303 None:intergenic 45.0%
ATCAAGGTCGGTGGAAAAGC+TGG + chr7.1:3369332-3369351 MS.gene050912:CDS 50.0%
CTTGCTCTTCTCACGGGTAA+CGG - chr7.1:3369378-3369397 None:intergenic 50.0%
GCCAGTGGAAGACAAGATCA+TGG + chr7.1:3369274-3369293 MS.gene050912:CDS 50.0%
GGTGCCAAGGGAAAGAAGAA+GGG + chr7.1:3369224-3369243 MS.gene050912:CDS 50.0%
TGCACCCTTCTTCTTTCCCT+TGG - chr7.1:3369231-3369250 None:intergenic 50.0%
! CATTGACTGTGCTAAGCCAG+TGG + chr7.1:3369259-3369278 MS.gene050912:CDS 50.0%
! TGGTGCCAAGGGAAAGAAGA+AGG + chr7.1:3369223-3369242 MS.gene050912:CDS 50.0%
!! CACTGGCTTAGCACAGTCAA+TGG - chr7.1:3369261-3369280 None:intergenic 50.0%
CAAGAGCGAATCAAGGTCGG+TGG + chr7.1:3369323-3369342 MS.gene050912:CDS 55.0%
CATTGCTGAGAACGAGGGAG+AGG + chr7.1:3370625-3370644 MS.gene050912:CDS 55.0%
GGTGGAAAAGCTGGTGCTCT+TGG + chr7.1:3369341-3369360 MS.gene050912:CDS 55.0%
! ATGAGTCGTGGTAACGCAGC+TGG + chr7.1:3369203-3369222 MS.gene050912:CDS 55.0%
! CTTCTCACGGGTAACGGTGA+CGG - chr7.1:3369372-3369391 None:intergenic 55.0%
! GGTAACGCAGCTGGTGCCAA+GGG + chr7.1:3369212-3369231 MS.gene050912:CDS 60.0%
! TGGTAACGCAGCTGGTGCCA+AGG + chr7.1:3369211-3369230 MS.gene050912:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 3369203 3370658 3369203 ID=MS.gene050912
chr7.1 mRNA 3369203 3370658 3369203 ID=MS.gene050912.t1;Parent=MS.gene050912
chr7.1 exon 3369203 3369432 3369203 ID=MS.gene050912.t1.exon1;Parent=MS.gene050912.t1
chr7.1 CDS 3369203 3369432 3369203 ID=cds.MS.gene050912.t1;Parent=MS.gene050912.t1
chr7.1 exon 3370514 3370658 3370514 ID=MS.gene050912.t1.exon2;Parent=MS.gene050912.t1
chr7.1 CDS 3370514 3370658 3370514 ID=cds.MS.gene050912.t1;Parent=MS.gene050912.t1
Gene Sequence

>MS.gene050912

ATGAGTCGTGGTAACGCAGCTGGTGCCAAGGGAAAGAAGAAGGGTGCAACCTTCACCATTGACTGTGCTAAGCCAGTGGAAGACAAGATCATGGACATTGCTTCACTTGAGAAGTTTCTTCAAGAGCGAATCAAGGTCGGTGGAAAAGCTGGTGCTCTTGGTGATTCCGTCACCGTTACCCGTGAGAAGAGCAAGATCACTGTTACCTCTGACAGTAACTTTTCCAAGCGGTAATAATTCATTTACAAATCATTGTTTGCTACTCTGGTTTTATCTCTTATGTTGCTTTGATTTAATATTTTATTGGTCATTACTTTGTTGATTTAGTTATATTCAGTGAAGATAAGTTATTGGCATGAAATCATGGTTCATTGCTTGGTTGATTTATTTATGGATGAATATTTTAAGATAGTTGATAGTCATTTTGGTTTCAATTTGTTGGTCTGTTTGGATTGGCTTATTTGAGCTTGTCTATGGGCATAAGTTTTGCGAAACTATTTGGGATAACTTACGAACAAGAGCTTATGGCACTCTTAGCTTACATAAGACACTTTTAGCTTACTTAATAAGTGTTCTTTAAGATAGGTTATGGAAATAACTTAGTGCTTATACAACGGCAATATAGGTCCTGTTTGGATAAACATCTAATTAATAGCTTATTGGTCAAGCGCTTAGCTTATCACAAATAAGCGCTTGTGCATTAGCTATTTAAAAAAAAAAAAAAAGTTAAATCAATGTTAAATTGTTTGTGTATTTGCTATGTTATAAGCTGTCTGAATAAGCTATATTGGTGTACATCTGAAAATAAGTTTAAAAAAGCTTATGAAGCTGTTTTCATAAGTTCTCTCAAGTAGTCTCACAAAATTTATGGCGGTAGATAAGCTCAATTAAGCCAATCTTAACAGTCCCATTACTCTATCATTTATCTTTATCAGCTTATACGTAGTGCCAATTTTAACAGTCCCATAACTATCATTTATCTTTACTAGCTTATACGTAGTGCTTATTTTTACTAGCTTATACGTAGTGCTTATTATGATAAGCACTTATGCTATAAGTCGCAAATAAGCTGTCTATCCCAACACGGCCTATATCTACAGAATATAGTATTGGGCTATCAGCAAACTAATTCATTGAAGGTTATTTTGTATATTTTGATTTTTTCTGAATGTAATGTATAGTACGATAACAACAGTATAAGGTCTACTTAATTGGACGAGAGCTGATATTTTGCTGTTTTTGGGCTTATTTAATTTGATTTCCGCAACTAATAACATAAGGTCTACTTGTTTAACATCTCACTGATTGTAGATACTTGAAGTATTTGACAAAGAAGTACTTGAAGAAACACAATGTAAGAGACTGGCTCAGAGTGATTGCTTCAAACAAAGATAGAAGTATCTATGAGCTCAGGTACTTCAACATTGCTGAGAACGAGGGAGAGGAAGAAGATTAA

Protein sequence

>MS.gene050912.t1

MSRGNAAGAKGKKKGATFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVTVTREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRSIYELRYFNIAENEGEEED