Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057272.t1 | XP_003602417.1 | 99.5 | 193 | 1 | 0 | 1 | 193 | 1 | 193 | 3.80E-101 | 377.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057272.t1 | P30707 | 96.9 | 193 | 6 | 0 | 1 | 193 | 1 | 193 | 2.5e-101 | 369.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057272.t1 | G7J9M6 | 99.5 | 193 | 1 | 0 | 1 | 193 | 1 | 193 | 2.7e-101 | 377.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene057272 | 0.80062 | 1.32E-48 | -1.69E-46 |
| MS.gene049387 | MS.gene057272 | 0.836893 | 7.17E-57 | -1.69E-46 |
| MS.gene050134 | MS.gene057272 | 0.929109 | 1.11E-92 | -1.69E-46 |
| MS.gene050252 | MS.gene057272 | 0.874634 | 5.80E-68 | -1.69E-46 |
| MS.gene050791 | MS.gene057272 | 0.913867 | 3.74E-84 | -1.69E-46 |
| MS.gene050912 | MS.gene057272 | 0.873755 | 1.15E-67 | -1.69E-46 |
| MS.gene051904 | MS.gene057272 | 0.832856 | 7.44E-56 | -1.69E-46 |
| MS.gene051984 | MS.gene057272 | 0.843392 | 1.44E-58 | -1.69E-46 |
| MS.gene052664 | MS.gene057272 | 0.85281 | 3.62E-61 | -1.69E-46 |
| MS.gene052666 | MS.gene057272 | 0.853864 | 1.80E-61 | -1.69E-46 |
| MS.gene053224 | MS.gene057272 | 0.896853 | 2.48E-76 | -1.69E-46 |
| MS.gene054624 | MS.gene057272 | 0.833253 | 5.93E-56 | -1.69E-46 |
| MS.gene057092 | MS.gene057272 | 0.804384 | 2.22E-49 | -1.69E-46 |
| MS.gene057272 | MS.gene057641 | 0.847164 | 1.38E-59 | -1.69E-46 |
| MS.gene057272 | MS.gene057643 | 0.883268 | 5.18E-71 | -1.69E-46 |
| MS.gene057272 | MS.gene058994 | 0.824082 | 9.79E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene059340 | 0.851117 | 1.09E-60 | -1.69E-46 |
| MS.gene057272 | MS.gene060483 | 0.863329 | 2.69E-64 | -1.69E-46 |
| MS.gene057272 | MS.gene06169 | 0.886228 | 4.10E-72 | -1.69E-46 |
| MS.gene057272 | MS.gene063093 | 0.843089 | 1.74E-58 | -1.69E-46 |
| MS.gene057272 | MS.gene06433 | 0.81882 | 1.61E-52 | -1.69E-46 |
| MS.gene057272 | MS.gene06581 | 0.917006 | 8.98E-86 | -1.69E-46 |
| MS.gene057272 | MS.gene06583 | 0.889941 | 1.54E-73 | -1.69E-46 |
| MS.gene057272 | MS.gene065917 | 0.808057 | 3.73E-50 | -1.69E-46 |
| MS.gene057272 | MS.gene06617 | 0.841875 | 3.64E-58 | -1.69E-46 |
| MS.gene057272 | MS.gene066583 | 0.824147 | 9.45E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene066811 | 0.852834 | 3.56E-61 | -1.69E-46 |
| MS.gene057272 | MS.gene067141 | 0.811991 | 5.31E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene067331 | 0.888066 | 8.21E-73 | -1.69E-46 |
| MS.gene057272 | MS.gene06803 | 0.939365 | 1.44E-99 | -1.69E-46 |
| MS.gene057272 | MS.gene06833 | 0.841951 | 3.48E-58 | -1.69E-46 |
| MS.gene057272 | MS.gene06999 | 0.819619 | 1.06E-52 | -1.69E-46 |
| MS.gene057272 | MS.gene07018 | 0.828025 | 1.13E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene070946 | 0.888221 | 7.16E-73 | -1.69E-46 |
| MS.gene057272 | MS.gene072093 | 0.863693 | 2.08E-64 | -1.69E-46 |
| MS.gene057272 | MS.gene072503 | 0.878374 | 2.96E-69 | -1.69E-46 |
| MS.gene057272 | MS.gene072589 | 0.90776 | 3.58E-81 | -1.69E-46 |
| MS.gene057272 | MS.gene072591 | 0.896465 | 3.59E-76 | -1.69E-46 |
| MS.gene057272 | MS.gene073358 | 0.837376 | 5.39E-57 | -1.69E-46 |
| MS.gene057272 | MS.gene073911 | 0.81867 | 1.74E-52 | -1.69E-46 |
| MS.gene057272 | MS.gene074400 | 0.84669 | 1.85E-59 | -1.69E-46 |
| MS.gene057272 | MS.gene08521 | 0.910401 | 1.95E-82 | -1.69E-46 |
| MS.gene057272 | MS.gene08869 | 0.911659 | 4.73E-83 | -1.69E-46 |
| MS.gene057272 | MS.gene08870 | 0.912108 | 2.84E-83 | -1.69E-46 |
| MS.gene057272 | MS.gene08871 | 0.915147 | 8.32E-85 | -1.69E-46 |
| MS.gene057272 | MS.gene09038 | 0.855345 | 6.71E-62 | -1.69E-46 |
| MS.gene057272 | MS.gene09204 | 0.858273 | 9.22E-63 | -1.69E-46 |
| MS.gene057272 | MS.gene09761 | 0.813848 | 2.08E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene09779 | 0.814186 | 1.75E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene09780 | 0.825132 | 5.54E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene20153 | 0.877829 | 4.59E-69 | -1.69E-46 |
| MS.gene057272 | MS.gene20766 | 0.834107 | 3.63E-56 | -1.69E-46 |
| MS.gene057272 | MS.gene21355 | 0.819914 | 9.04E-53 | -1.69E-46 |
| MS.gene057272 | MS.gene22507 | 0.812832 | 3.48E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene22784 | 0.900576 | 6.38E-78 | -1.69E-46 |
| MS.gene057272 | MS.gene23031 | 0.855175 | 7.52E-62 | -1.69E-46 |
| MS.gene057272 | MS.gene23303 | 0.893916 | 4.02E-75 | -1.69E-46 |
| MS.gene057272 | MS.gene23437 | 0.825555 | 4.40E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene24232 | 0.873514 | 1.39E-67 | -1.69E-46 |
| MS.gene057272 | MS.gene24639 | 0.890998 | 5.93E-74 | -1.69E-46 |
| MS.gene057272 | MS.gene25236 | 0.880522 | 5.12E-70 | -1.69E-46 |
| MS.gene057272 | MS.gene25639 | 0.880994 | 3.47E-70 | -1.69E-46 |
| MS.gene057272 | MS.gene25903 | 0.895978 | 5.73E-76 | -1.69E-46 |
| MS.gene057272 | MS.gene26343 | 0.910402 | 1.95E-82 | -1.69E-46 |
| MS.gene057272 | MS.gene26707 | 0.848269 | 6.82E-60 | -1.69E-46 |
| MS.gene057272 | MS.gene27003 | 0.831053 | 2.08E-55 | -1.69E-46 |
| MS.gene057272 | MS.gene27265 | 0.898364 | 5.70E-77 | -1.69E-46 |
| MS.gene057272 | MS.gene28147 | 0.892854 | 1.08E-74 | -1.69E-46 |
| MS.gene057272 | MS.gene28568 | 0.809518 | 1.82E-50 | -1.69E-46 |
| MS.gene057272 | MS.gene30933 | 0.81449 | 1.50E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene30990 | 0.832224 | 1.07E-55 | -1.69E-46 |
| MS.gene057272 | MS.gene31039 | 0.853354 | 2.53E-61 | -1.69E-46 |
| MS.gene057272 | MS.gene31991 | 0.863836 | 1.87E-64 | -1.69E-46 |
| MS.gene057272 | MS.gene32591 | 0.832953 | 7.04E-56 | -1.69E-46 |
| MS.gene057272 | MS.gene34372 | 0.911857 | 3.78E-83 | -1.69E-46 |
| MS.gene057272 | MS.gene36675 | 0.805045 | 1.61E-49 | -1.69E-46 |
| MS.gene057272 | MS.gene37037 | 0.811612 | 6.42E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene38035 | 0.854843 | 9.40E-62 | -1.69E-46 |
| MS.gene057272 | MS.gene38222 | 0.879301 | 1.39E-69 | -1.69E-46 |
| MS.gene057272 | MS.gene38735 | 0.839307 | 1.71E-57 | -1.69E-46 |
| MS.gene057272 | MS.gene43070 | 0.824425 | 8.13E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene43071 | 0.818661 | 1.74E-52 | -1.69E-46 |
| MS.gene057272 | MS.gene46387 | 0.863737 | 2.01E-64 | -1.69E-46 |
| MS.gene057272 | MS.gene46907 | 0.804672 | 1.93E-49 | -1.69E-46 |
| MS.gene057272 | MS.gene47371 | 0.81038 | 1.19E-50 | -1.69E-46 |
| MS.gene057272 | MS.gene48048 | 0.814433 | 1.54E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene49109 | 0.809784 | 1.59E-50 | -1.69E-46 |
| MS.gene057272 | MS.gene50640 | 0.921344 | 4.03E-88 | -1.69E-46 |
| MS.gene057272 | MS.gene50756 | 0.888694 | 4.70E-73 | -1.69E-46 |
| MS.gene057272 | MS.gene50758 | 0.874922 | 4.62E-68 | -1.69E-46 |
| MS.gene057272 | MS.gene51534 | 0.826783 | 2.24E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene53465 | 0.815559 | 8.69E-52 | -1.69E-46 |
| MS.gene057272 | MS.gene54437 | 0.818338 | 2.06E-52 | -1.69E-46 |
| MS.gene057272 | MS.gene55619 | 0.914341 | 2.15E-84 | -1.69E-46 |
| MS.gene057272 | MS.gene55885 | 0.802008 | 6.88E-49 | -1.69E-46 |
| MS.gene057272 | MS.gene55890 | 0.924071 | 1.15E-89 | -1.69E-46 |
| MS.gene057272 | MS.gene58173 | 0.858141 | 1.01E-62 | -1.69E-46 |
| MS.gene057272 | MS.gene59228 | 0.894956 | 1.51E-75 | -1.69E-46 |
| MS.gene057272 | MS.gene59230 | 0.842981 | 1.85E-58 | -1.69E-46 |
| MS.gene057272 | MS.gene59457 | 0.814789 | 1.29E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene59609 | 0.897062 | 2.02E-76 | -1.69E-46 |
| MS.gene057272 | MS.gene60693 | 0.806403 | 8.37E-50 | -1.69E-46 |
| MS.gene057272 | MS.gene61251 | 0.818799 | 1.62E-52 | -1.69E-46 |
| MS.gene057272 | MS.gene62561 | 0.840918 | 6.51E-58 | -1.69E-46 |
| MS.gene057272 | MS.gene62620 | 0.85499 | 8.52E-62 | -1.69E-46 |
| MS.gene057272 | MS.gene62791 | 0.857832 | 1.25E-62 | -1.69E-46 |
| MS.gene057272 | MS.gene63973 | 0.821755 | 3.41E-53 | -1.69E-46 |
| MS.gene057272 | MS.gene64754 | 0.858443 | 8.21E-63 | -1.69E-46 |
| MS.gene057272 | MS.gene68049 | 0.824915 | 6.23E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene68740 | 0.832084 | 1.16E-55 | -1.69E-46 |
| MS.gene057272 | MS.gene70316 | 0.930434 | 1.65E-93 | -1.69E-46 |
| MS.gene057272 | MS.gene70579 | 0.88563 | 6.89E-72 | -1.69E-46 |
| MS.gene057272 | MS.gene72382 | 0.876141 | 1.77E-68 | -1.69E-46 |
| MS.gene057272 | MS.gene73000 | 0.864135 | 1.51E-64 | -1.69E-46 |
| MS.gene057272 | MS.gene74837 | 0.809172 | 2.16E-50 | -1.69E-46 |
| MS.gene057272 | MS.gene76031 | 0.838499 | 2.77E-57 | -1.69E-46 |
| MS.gene057272 | MS.gene79721 | 0.928491 | 2.68E-92 | -1.69E-46 |
| MS.gene057272 | MS.gene81099 | 0.822359 | 2.47E-53 | -1.69E-46 |
| MS.gene057272 | MS.gene82009 | 0.832366 | 9.85E-56 | -1.69E-46 |
| MS.gene057272 | MS.gene82038 | 0.918268 | 1.92E-86 | -1.69E-46 |
| MS.gene057272 | MS.gene82545 | 0.91948 | 4.27E-87 | -1.69E-46 |
| MS.gene057272 | MS.gene83175 | 0.839208 | 1.82E-57 | -1.69E-46 |
| MS.gene057272 | MS.gene88081 | 0.846131 | 2.63E-59 | -1.69E-46 |
| MS.gene057272 | MS.gene88918 | 0.877692 | 5.13E-69 | -1.69E-46 |
| MS.gene057272 | MS.gene89608 | 0.814719 | 1.34E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene90945 | 0.8617 | 8.53E-64 | -1.69E-46 |
| MS.gene057272 | MS.gene92142 | 0.904936 | 7.28E-80 | -1.69E-46 |
| MS.gene057272 | MS.gene93783 | 0.806763 | 7.02E-50 | -1.69E-46 |
| MS.gene057272 | MS.gene95803 | 0.881644 | 2.02E-70 | -1.69E-46 |
| MS.gene057272 | MS.gene96194 | 0.800172 | 1.63E-48 | -1.69E-46 |
| MS.gene057272 | MS.gene96225 | 0.90347 | 3.36E-79 | -1.69E-46 |
| MS.gene057272 | MS.gene96825 | 0.852155 | 5.56E-61 | -1.69E-46 |
| MS.gene057272 | MS.gene98171 | 0.904213 | 1.55E-79 | -1.69E-46 |
| MS.gene057272 | MS.gene98978 | 0.818715 | 1.70E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene06583 | MS.gene057272 | PPI |
| MS.gene77045 | MS.gene057272 | PPI |
| MS.gene057272 | MS.gene013477 | PPI |
| MS.gene06581 | MS.gene057272 | PPI |
| MS.gene42075 | MS.gene057272 | PPI |
| MS.gene87743 | MS.gene057272 | PPI |
| MS.gene87064 | MS.gene057272 | PPI |
| MS.gene43711 | MS.gene057272 | PPI |
| MS.gene76728 | MS.gene057272 | PPI |
| MS.gene82237 | MS.gene057272 | PPI |
| MS.gene48819 | MS.gene057272 | PPI |
| MS.gene06803 | MS.gene057272 | PPI |
| MS.gene057272 | MS.gene06583 | PPI |
| MS.gene013477 | MS.gene057272 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057272.t1 | MTR_3g093110 | 99.482 | 193 | 1 | 0 | 1 | 193 | 1 | 193 | 7.34e-139 | 385 |
| MS.gene057272.t1 | MTR_5g068780 | 87.500 | 192 | 24 | 0 | 1 | 192 | 1 | 192 | 1.36e-126 | 354 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057272.t1 | AT1G33140 | 87.113 | 194 | 24 | 1 | 1 | 193 | 1 | 194 | 9.38e-122 | 342 |
| MS.gene057272.t1 | AT1G33120 | 87.113 | 194 | 24 | 1 | 1 | 193 | 1 | 194 | 9.38e-122 | 342 |
| MS.gene057272.t1 | AT4G10450 | 84.536 | 194 | 29 | 1 | 1 | 193 | 1 | 194 | 2.52e-120 | 338 |
| MS.gene057272.t1 | AT4G10450 | 84.337 | 166 | 25 | 1 | 1 | 165 | 1 | 166 | 2.38e-101 | 290 |
Find 53 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGAGATCCGAAACTTTCT+TGG | 0.238218 | 3.2:+78264751 | MS.gene057272:CDS |
| GGTGAAGGATGAGGTTGTTT+TGG | 0.319250 | 3.2:+78265222 | MS.gene057272:CDS |
| ATGACATTGAGCTTGTTTCT+AGG | 0.326351 | 3.2:+78265251 | MS.gene057272:CDS |
| TCGATGCCTGGTTCGGTTCC+AGG | 0.334604 | 3.2:+78264587 | MS.gene057272:CDS |
| GTCAGGGATGTTCATGGTTT+CGG | 0.351741 | 3.2:-78264431 | None:intergenic |
| TCTTCTCTCCAAGAAAGTTT+CGG | 0.365793 | 3.2:-78264759 | None:intergenic |
| ACCTTGATGCTAACGCCGTC+AGG | 0.384134 | 3.2:-78264448 | None:intergenic |
| CTTCTTCTTGCCTTCTTCAT+CGG | 0.396628 | 3.2:-78264557 | None:intergenic |
| CTCAATCGACTTGTTGTCAT+TGG | 0.397158 | 3.2:-78264734 | None:intergenic |
| GGTATTTATGTTAGTGAGAA+GGG | 0.433044 | 3.2:+78265433 | MS.gene057272:CDS |
| ATTGGTGATGCTAGCGTTGA+TGG | 0.438976 | 3.2:-78264716 | None:intergenic |
| ATGTTAAGAAGAAGGATATC+AGG | 0.444332 | 3.2:+78265398 | MS.gene057272:CDS |
| CTCAAGATCGATGCCTGGTT+CGG | 0.447724 | 3.2:+78264580 | MS.gene057272:CDS |
| GATATCAGGAAGTTTCTTGA+TGG | 0.450730 | 3.2:+78265412 | MS.gene057272:CDS |
| AGGAAAGTAGATTTGCTTGA+TGG | 0.454716 | 3.2:+78265175 | MS.gene057272:CDS |
| AGCAGTTCTGATGGCGGCGG+AGG | 0.457127 | 3.2:-78264614 | None:intergenic |
| AACGCCGTCAGGGATGTTCA+TGG | 0.489928 | 3.2:-78264437 | None:intergenic |
| AAACTTTCTTGGAGAGAAGA+AGG | 0.493598 | 3.2:+78264762 | MS.gene057272:CDS |
| GATCTCATCACCGATGAAGA+AGG | 0.495068 | 3.2:+78264547 | MS.gene057272:CDS |
| GAAATGCCATGTTAAGAAGA+AGG | 0.499506 | 3.2:+78265390 | MS.gene057272:intron |
| CCCTGACGGCGTTAGCATCA+AGG | 0.507605 | 3.2:+78264447 | MS.gene057272:CDS |
| TTCAATTGTTCGATCTGAGA+AGG | 0.526562 | 3.2:+78265201 | MS.gene057272:CDS |
| TGGTATTTATGTTAGTGAGA+AGG | 0.531892 | 3.2:+78265432 | MS.gene057272:CDS |
| TGATATCCTTCTTCTTAACA+TGG | 0.532081 | 3.2:-78265396 | None:intergenic |
| CATGGTTTCGGAGGAGAGGA+TGG | 0.534778 | 3.2:-78264419 | None:intergenic |
| TTGGTGATGCTAGCGTTGAT+GGG | 0.543746 | 3.2:-78264715 | None:intergenic |
| TTATGTTAGTGAGAAGGGAT+CGG | 0.552761 | 3.2:+78265438 | MS.gene057272:CDS |
| CACGTTGAGAATCTCATCAC+TGG | 0.554104 | 3.2:+78264643 | MS.gene057272:CDS |
| CATCTTGTAGCGGAAACCCT+TGG | 0.556289 | 3.2:-78264671 | None:intergenic |
| AGGGTTTCCGCTACAAGATG+AGG | 0.564650 | 3.2:+78264674 | MS.gene057272:CDS |
| ATCTGAGAAGGTGAAGGATG+AGG | 0.567425 | 3.2:+78265213 | MS.gene057272:CDS |
| GAGAGCAGTTCTGATGGCGG+CGG | 0.574544 | 3.2:-78264617 | None:intergenic |
| CTCATCACTGGTGTCACCAA+GGG | 0.576975 | 3.2:+78264655 | MS.gene057272:CDS |
| GTGGCTGAGAGCAGTTCTGA+TGG | 0.581831 | 3.2:-78264623 | None:intergenic |
| TGTTCATGGTTTCGGAGGAG+AGG | 0.584113 | 3.2:-78264423 | None:intergenic |
| TAGCATCAAGGTTAACGCTA+AGG | 0.586158 | 3.2:+78264459 | MS.gene057272:CDS |
| AGAAGCTCAAGATCGATGCC+TGG | 0.588777 | 3.2:+78264575 | MS.gene057272:CDS |
| CCTTGATGCTAACGCCGTCA+GGG | 0.590861 | 3.2:-78264447 | None:intergenic |
| TCGAAGTCGAAGGACCTCGC+GGG | 0.613305 | 3.2:+78264485 | MS.gene057272:CDS |
| AGGGATGTTCATGGTTTCGG+AGG | 0.621327 | 3.2:-78264428 | None:intergenic |
| TCTCATCACTGGTGTCACCA+AGG | 0.623987 | 3.2:+78264654 | MS.gene057272:CDS |
| GTCATGTGCACTTATTAACC+AGG | 0.625933 | 3.2:+78265273 | MS.gene057272:CDS |
| TGTTCGATCTGAGAAGGTGA+AGG | 0.628133 | 3.2:+78265207 | MS.gene057272:CDS |
| GCTAAGGTCATCGAAGTCGA+AGG | 0.640211 | 3.2:+78264475 | MS.gene057272:CDS |
| TGTTAGTGAGAAGGGATCGG+TGG | 0.651106 | 3.2:+78265441 | MS.gene057272:CDS |
| ATCGAAGTCGAAGGACCTCG+CGG | 0.660265 | 3.2:+78264484 | MS.gene057272:CDS |
| TGGTGATGCTAGCGTTGATG+GGG | 0.676063 | 3.2:-78264714 | None:intergenic |
| GCTGAGAGCAGTTCTGATGG+CGG | 0.677632 | 3.2:-78264620 | None:intergenic |
| CGGTGATGAGATCAAAATCG+AGG | 0.680504 | 3.2:-78264537 | None:intergenic |
| AGACAAACCTCATCTTGTAG+CGG | 0.682924 | 3.2:-78264681 | None:intergenic |
| GAAACCATGAACATCCCTGA+CGG | 0.701649 | 3.2:+78264433 | MS.gene057272:CDS |
| CAGTGATGAGATTCTCAACG+TGG | 0.725921 | 3.2:-78264642 | None:intergenic |
| TCACGAACAAGCTTCCCGCG+AGG | 0.748381 | 3.2:-78264499 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAATTAGATTAAAACTA+GGG | - | chr3.2:78264900-78264919 | None:intergenic | 10.0% |
| !! | TTATAAATTAGATTAAAACT+AGG | - | chr3.2:78264901-78264920 | None:intergenic | 10.0% |
| !!! | ATGAGTTAATTTTTTTATTT+TGG | + | chr3.2:78265043-78265062 | MS.gene057272:intron | 10.0% |
| !!! | TCTTTAATTTCTTCAATTTT+AGG | + | chr3.2:78264817-78264836 | MS.gene057272:intron | 15.0% |
| !! | GAGATAATGTCTAATTTATT+TGG | + | chr3.2:78265077-78265096 | MS.gene057272:intron | 20.0% |
| ! | ACAAAAATAAAGCATGTTCA+TGG | - | chr3.2:78265317-78265336 | None:intergenic | 25.0% |
| ! | CACAAATTAAAGTGAAACTA+AGG | - | chr3.2:78264851-78264870 | None:intergenic | 25.0% |
| ! | GAATCGAATAATGTTGTATT+AGG | + | chr3.2:78265150-78265169 | MS.gene057272:intron | 25.0% |
| ! | GTCTAATTTATTTGGTTATC+TGG | + | chr3.2:78265085-78265104 | MS.gene057272:intron | 25.0% |
| ATGTTAAGAAGAAGGATATC+AGG | + | chr3.2:78265398-78265417 | MS.gene057272:CDS | 30.0% | |
| GAATAATGTTGTATTAGGTG+AGG | + | chr3.2:78265155-78265174 | MS.gene057272:intron | 30.0% | |
| GGTATTTATGTTAGTGAGAA+GGG | + | chr3.2:78265433-78265452 | MS.gene057272:CDS | 30.0% | |
| TGATATCCTTCTTCTTAACA+TGG | - | chr3.2:78265399-78265418 | None:intergenic | 30.0% | |
| TGGTATTTATGTTAGTGAGA+AGG | + | chr3.2:78265432-78265451 | MS.gene057272:CDS | 30.0% | |
| ! | TTTACTAGTTTGTGAATCTG+TGG | + | chr3.2:78265123-78265142 | MS.gene057272:intron | 30.0% |
| !! | GAATGTTGTTGTTGCATTTT+TGG | + | chr3.2:78265363-78265382 | MS.gene057272:intron | 30.0% |
| AAACTTTCTTGGAGAGAAGA+AGG | + | chr3.2:78264762-78264781 | MS.gene057272:CDS | 35.0% | |
| ATGTTCATGGACCATGTTAA+GGG | - | chr3.2:78265304-78265323 | None:intergenic | 35.0% | |
| ATTGAGATCCGAAACTTTCT+TGG | + | chr3.2:78264751-78264770 | MS.gene057272:CDS | 35.0% | |
| GAAATGCCATGTTAAGAAGA+AGG | + | chr3.2:78265390-78265409 | MS.gene057272:intron | 35.0% | |
| GATATCAGGAAGTTTCTTGA+TGG | + | chr3.2:78265412-78265431 | MS.gene057272:CDS | 35.0% | |
| TCTTCTCTCCAAGAAAGTTT+CGG | - | chr3.2:78264762-78264781 | None:intergenic | 35.0% | |
| TTATGTTAGTGAGAAGGGAT+CGG | + | chr3.2:78265438-78265457 | MS.gene057272:CDS | 35.0% | |
| TTCAATTGTTCGATCTGAGA+AGG | + | chr3.2:78265201-78265220 | MS.gene057272:CDS | 35.0% | |
| ! | AGGAAAGTAGATTTGCTTGA+TGG | + | chr3.2:78265175-78265194 | MS.gene057272:CDS | 35.0% |
| ! | ATGACATTGAGCTTGTTTCT+AGG | + | chr3.2:78265251-78265270 | MS.gene057272:CDS | 35.0% |
| AGACAAACCTCATCTTGTAG+CGG | - | chr3.2:78264684-78264703 | None:intergenic | 40.0% | |
| CATGTTCATGGACCATGTTA+AGG | - | chr3.2:78265305-78265324 | None:intergenic | 40.0% | |
| CTTCTTCTTGCCTTCTTCAT+CGG | - | chr3.2:78264560-78264579 | None:intergenic | 40.0% | |
| GTCATGTGCACTTATTAACC+AGG | + | chr3.2:78265273-78265292 | MS.gene057272:CDS | 40.0% | |
| TAGCATCAAGGTTAACGCTA+AGG | + | chr3.2:78264459-78264478 | MS.gene057272:CDS | 40.0% | |
| TGTTCATGGACCATGTTAAG+GGG | - | chr3.2:78265303-78265322 | None:intergenic | 40.0% | |
| !! | CTCAATCGACTTGTTGTCAT+TGG | - | chr3.2:78264737-78264756 | None:intergenic | 40.0% |
| !!! | AAGGATGAGGTTGTTTTGGA+TGG | + | chr3.2:78265226-78265245 | MS.gene057272:CDS | 40.0% |
| ATCTGAGAAGGTGAAGGATG+AGG | + | chr3.2:78265213-78265232 | MS.gene057272:CDS | 45.0% | |
| CACGTTGAGAATCTCATCAC+TGG | + | chr3.2:78264643-78264662 | MS.gene057272:CDS | 45.0% | |
| CAGTGATGAGATTCTCAACG+TGG | - | chr3.2:78264645-78264664 | None:intergenic | 45.0% | |
| CGGTGATGAGATCAAAATCG+AGG | - | chr3.2:78264540-78264559 | None:intergenic | 45.0% | |
| GAAACCATGAACATCCCTGA+CGG | + | chr3.2:78264433-78264452 | MS.gene057272:CDS | 45.0% | |
| GATCTCATCACCGATGAAGA+AGG | + | chr3.2:78264547-78264566 | MS.gene057272:CDS | 45.0% | |
| GTCAGGGATGTTCATGGTTT+CGG | - | chr3.2:78264434-78264453 | None:intergenic | 45.0% | |
| TGTTCGATCTGAGAAGGTGA+AGG | + | chr3.2:78265207-78265226 | MS.gene057272:CDS | 45.0% | |
| ! | GGTGAAGGATGAGGTTGTTT+TGG | + | chr3.2:78265222-78265241 | MS.gene057272:CDS | 45.0% |
| !! | ATTGGTGATGCTAGCGTTGA+TGG | - | chr3.2:78264719-78264738 | None:intergenic | 45.0% |
| !! | TTGGTGATGCTAGCGTTGAT+GGG | - | chr3.2:78264718-78264737 | None:intergenic | 45.0% |
| ACCAGGTACACCCCTTAACA+TGG | + | chr3.2:78265290-78265309 | MS.gene057272:intron | 50.0% | |
| AGAAGCTCAAGATCGATGCC+TGG | + | chr3.2:78264575-78264594 | MS.gene057272:CDS | 50.0% | |
| AGGGATGTTCATGGTTTCGG+AGG | - | chr3.2:78264431-78264450 | None:intergenic | 50.0% | |
| AGGGTTTCCGCTACAAGATG+AGG | + | chr3.2:78264674-78264693 | MS.gene057272:CDS | 50.0% | |
| CTCAAGATCGATGCCTGGTT+CGG | + | chr3.2:78264580-78264599 | MS.gene057272:CDS | 50.0% | |
| GCTAAGGTCATCGAAGTCGA+AGG | + | chr3.2:78264475-78264494 | MS.gene057272:CDS | 50.0% | |
| TGTTAGTGAGAAGGGATCGG+TGG | + | chr3.2:78265441-78265460 | MS.gene057272:CDS | 50.0% | |
| TGTTCATGGTTTCGGAGGAG+AGG | - | chr3.2:78264426-78264445 | None:intergenic | 50.0% | |
| ! | ACCATGTTAAGGGGTGTACC+TGG | - | chr3.2:78265294-78265313 | None:intergenic | 50.0% |
| ! | CATCTTGTAGCGGAAACCCT+TGG | - | chr3.2:78264674-78264693 | None:intergenic | 50.0% |
| !! | CTCATCACTGGTGTCACCAA+GGG | + | chr3.2:78264655-78264674 | MS.gene057272:CDS | 50.0% |
| !! | TCTCATCACTGGTGTCACCA+AGG | + | chr3.2:78264654-78264673 | MS.gene057272:CDS | 50.0% |
| !! | TGGTGATGCTAGCGTTGATG+GGG | - | chr3.2:78264717-78264736 | None:intergenic | 50.0% |
| AACGCCGTCAGGGATGTTCA+TGG | - | chr3.2:78264440-78264459 | None:intergenic | 55.0% | |
| ACCTTGATGCTAACGCCGTC+AGG | - | chr3.2:78264451-78264470 | None:intergenic | 55.0% | |
| ATCGAAGTCGAAGGACCTCG+CGG | + | chr3.2:78264484-78264503 | MS.gene057272:CDS | 55.0% | |
| CATGGTTTCGGAGGAGAGGA+TGG | - | chr3.2:78264422-78264441 | None:intergenic | 55.0% | |
| CCTTGATGCTAACGCCGTCA+GGG | - | chr3.2:78264450-78264469 | None:intergenic | 55.0% | |
| ! | GCTGAGAGCAGTTCTGATGG+CGG | - | chr3.2:78264623-78264642 | None:intergenic | 55.0% |
| ! | GGTTTTCCTGGAACCGAACC+AGG | - | chr3.2:78264596-78264615 | None:intergenic | 55.0% |
| ! | GTGGCTGAGAGCAGTTCTGA+TGG | - | chr3.2:78264626-78264645 | None:intergenic | 55.0% |
| CCCTGACGGCGTTAGCATCA+AGG | + | chr3.2:78264447-78264466 | MS.gene057272:CDS | 60.0% | |
| TCACGAACAAGCTTCCCGCG+AGG | - | chr3.2:78264502-78264521 | None:intergenic | 60.0% | |
| TCGAAGTCGAAGGACCTCGC+GGG | + | chr3.2:78264485-78264504 | MS.gene057272:CDS | 60.0% | |
| !! | GAGAGCAGTTCTGATGGCGG+CGG | - | chr3.2:78264620-78264639 | None:intergenic | 60.0% |
| !! | TCGATGCCTGGTTCGGTTCC+AGG | + | chr3.2:78264587-78264606 | MS.gene057272:CDS | 60.0% |
| !! | AGCAGTTCTGATGGCGGCGG+AGG | - | chr3.2:78264617-78264636 | None:intergenic | 65.0% |
| !! | GATGGCGGCGGAGGTTTTCC+TGG | - | chr3.2:78264608-78264627 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 78264412 | 78265477 | 78264412 | ID=MS.gene057272 |
| chr3.2 | mRNA | 78264412 | 78265477 | 78264412 | ID=MS.gene057272.t1;Parent=MS.gene057272 |
| chr3.2 | exon | 78264412 | 78264783 | 78264412 | ID=MS.gene057272.t1.exon1;Parent=MS.gene057272.t1 |
| chr3.2 | CDS | 78264412 | 78264783 | 78264412 | ID=cds.MS.gene057272.t1;Parent=MS.gene057272.t1 |
| chr3.2 | exon | 78265172 | 78265294 | 78265172 | ID=MS.gene057272.t1.exon2;Parent=MS.gene057272.t1 |
| chr3.2 | CDS | 78265172 | 78265294 | 78265172 | ID=cds.MS.gene057272.t1;Parent=MS.gene057272.t1 |
| chr3.2 | exon | 78265391 | 78265477 | 78265391 | ID=MS.gene057272.t1.exon3;Parent=MS.gene057272.t1 |
| chr3.2 | CDS | 78265391 | 78265477 | 78265391 | ID=cds.MS.gene057272.t1;Parent=MS.gene057272.t1 |
| Gene Sequence |
| Protein sequence |
