Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066342.t1 | TKY54179.1 | 62.1 | 182 | 32 | 4 | 1 | 145 | 4 | 185 | 3.10E-47 | 198 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066342.t1 | B7FGK2 | 62.1 | 177 | 35 | 2 | 1 | 145 | 3 | 179 | 1.1e-41 | 179.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049424 | MS.gene066342 | 0.801515 | 8.68E-49 | -1.69E-46 |
| MS.gene051122 | MS.gene066342 | 0.81819 | 2.23E-52 | -1.69E-46 |
| MS.gene051452 | MS.gene066342 | 0.800454 | 1.43E-48 | -1.69E-46 |
| MS.gene054536 | MS.gene066342 | 0.80963 | 1.72E-50 | -1.69E-46 |
| MS.gene057108 | MS.gene066342 | 0.808191 | 3.50E-50 | -1.69E-46 |
| MS.gene057109 | MS.gene066342 | 0.807606 | 4.66E-50 | -1.69E-46 |
| MS.gene057110 | MS.gene066342 | 0.809527 | 1.81E-50 | -1.69E-46 |
| MS.gene060297 | MS.gene066342 | 0.807553 | 4.78E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066342.t1 | MTR_4g120400 | 80.226 | 177 | 3 | 2 | 1 | 145 | 3 | 179 | 1.44e-79 | 233 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGGAATTTTAATAGATTT+GGG | - | 32540:13677-13696 | MS.gene066342:CDS | 15.0% |
| !!! | GATTGGAATTTTAATAGATT+TGG | - | 32540:13676-13695 | MS.gene066342:CDS | 20.0% |
| !!! | TTGGAATTTTAATAGATTTG+GGG | - | 32540:13678-13697 | MS.gene066342:CDS | 20.0% |
| !!! | AATTTTAATAGATTTGGGGA+GGG | - | 32540:13682-13701 | MS.gene066342:CDS | 25.0% |
| !!! | TTCTATTGTTGATTCTGATA+GGG | - | 32540:13837-13856 | MS.gene066342:CDS | 25.0% |
| ATTGTGAGAATTATTGCAGA+TGG | - | 32540:13814-13833 | MS.gene066342:CDS | 30.0% | |
| !! | GAATTTTAATAGATTTGGGG+AGG | - | 32540:13681-13700 | MS.gene066342:CDS | 30.0% |
| !! | GTTCTATTGTTGATTCTGAT+AGG | - | 32540:13836-13855 | MS.gene066342:CDS | 30.0% |
| AATCATTGCTGAGATCTGAT+CGG | + | 32540:13452-13471 | None:intergenic | 35.0% | |
| ATCGAATTGTTTGCATTTCG+CGG | - | 32540:13783-13802 | MS.gene066342:CDS | 35.0% | |
| ! | AGCGTTTGATTTCGTTTATC+GGG | - | 32540:13741-13760 | MS.gene066342:CDS | 35.0% |
| ! | CAGCGTTTGATTTCGTTTAT+CGG | - | 32540:13740-13759 | MS.gene066342:CDS | 35.0% |
| !! | TGTTGATTCTGATAGGGAAA+AGG | - | 32540:13843-13862 | MS.gene066342:CDS | 35.0% |
| AATGATTCATCCTTGCGATG+CGG | - | 32540:13465-13484 | MS.gene066342:CDS | 40.0% | |
| ATCGGCGATGGAAAATTGAT+AGG | + | 32540:13422-13441 | None:intergenic | 40.0% | |
| CGATAAACGAAATCAAACGC+TGG | + | 32540:13742-13761 | None:intergenic | 40.0% | |
| GATCTGATCGGAAGATGAAT+CGG | + | 32540:13440-13459 | None:intergenic | 40.0% | |
| AAGTTGTCCGGCGATGATAA+CGG | - | 32540:13568-13587 | MS.gene066342:intron | 45.0% | |
| ATTTGGGGAGGGTGAGAATT+GGG | - | 32540:13693-13712 | MS.gene066342:CDS | 45.0% | |
| CAAATTGGAGCCAATCTAGC+TGG | + | 32540:13871-13890 | None:intergenic | 45.0% | |
| GGAAAAGGTTCCAGCTAGAT+TGG | - | 32540:13858-13877 | MS.gene066342:CDS | 45.0% | |
| TGGAAAATTGATAGGCGCGA+AGG | + | 32540:13414-13433 | None:intergenic | 45.0% | |
| ! | AAGGATGCAGAGACTTTTGG+CGG | + | 32540:13395-13414 | None:intergenic | 45.0% |
| ! | TCGTTTATCGGGTGTTATCG+TGG | - | 32540:13752-13771 | MS.gene066342:CDS | 45.0% |
| ATCGGAAGATGAATCGGCGA+TGG | + | 32540:13434-13453 | None:intergenic | 50.0% | |
| CGAACAAGACGGAAGTTGTC+CGG | - | 32540:13556-13575 | MS.gene066342:intron | 50.0% | |
| GAGGGGTTATTCTTCGGTGA+TGG | - | 32540:13595-13614 | MS.gene066342:intron | 50.0% | |
| GATTTGGGGAGGGTGAGAAT+TGG | - | 32540:13692-13711 | MS.gene066342:CDS | 50.0% | |
| GGAGGAAACGACGAACAAGA+CGG | - | 32540:13545-13564 | MS.gene066342:CDS | 50.0% | |
| GGAGGTGAGGGGTTATTCTT+CGG | - | 32540:13589-13608 | MS.gene066342:intron | 50.0% | |
| TCTTGTTCGTCGTTTCCTCC+GGG | + | 32540:13545-13564 | None:intergenic | 50.0% | |
| ! | GCGAAGGATGCAGAGACTTT+TGG | + | 32540:13398-13417 | None:intergenic | 50.0% |
| ! | GGGTTATTCTTCGGTGATGG+CGG | - | 32540:13598-13617 | MS.gene066342:intron | 50.0% |
| ! | GTCTTGTTCGTCGTTTCCTC+CGG | + | 32540:13546-13565 | None:intergenic | 50.0% |
| !! | GATGGCGGTGACGGTATTTT+CGG | - | 32540:13613-13632 | MS.gene066342:intron | 50.0% |
| CGTCCGACGTGGTATTCTGT+GGG | + | 32540:13518-13537 | None:intergenic | 55.0% | |
| CGTGGTATTCTGTGGGTGCT+AGG | + | 32540:13511-13530 | None:intergenic | 55.0% | |
| CTCACCTCCGTTATCATCGC+CGG | + | 32540:13578-13597 | None:intergenic | 55.0% | |
| CTGTGGGTGCTAGGTTTGAC+CGG | + | 32540:13502-13521 | None:intergenic | 55.0% | |
| GATTCATCCTTGCGATGCGG+TGG | - | 32540:13468-13487 | MS.gene066342:CDS | 55.0% | |
| GCACCCACAGAATACCACGT+CGG | - | 32540:13512-13531 | MS.gene066342:CDS | 55.0% | |
| ! | GAGACTTTTGGCGGCGGTAA+CGG | + | 32540:13386-13405 | None:intergenic | 55.0% |
| ! | GATGCAGAGACTTTTGGCGG+CGG | + | 32540:13392-13411 | None:intergenic | 55.0% |
| !! | GACGGTATTTTCGGTGGTGG+TGG | - | 32540:13622-13641 | MS.gene066342:intron | 55.0% |
| !! | GCGATGATAACGGAGGTGAG+GGG | - | 32540:13578-13597 | MS.gene066342:intron | 55.0% |
| !! | GGCGATGATAACGGAGGTGA+GGG | - | 32540:13577-13596 | MS.gene066342:intron | 55.0% |
| !! | GGTGACGGTATTTTCGGTGG+TGG | - | 32540:13619-13638 | MS.gene066342:intron | 55.0% |
| !! | TTCTTCGGTGATGGCGGTGA+CGG | - | 32540:13604-13623 | MS.gene066342:intron | 55.0% |
| !! | TTGTCCGGCGATGATAACGG+AGG | - | 32540:13571-13590 | MS.gene066342:intron | 55.0% |
| !!! | GGTATTTTCGGTGGTGGTGG+TGG | - | 32540:13625-13644 | MS.gene066342:intron | 55.0% |
| !!! | TTCGGTGGTGGTGGTGGTTT+TGG | - | 32540:13631-13650 | MS.gene066342:intron | 55.0% |
| GCGTCCGACGTGGTATTCTG+TGG | + | 32540:13519-13538 | None:intergenic | 60.0% | |
| GCTGGATCCTGCAACGACGA+AGG | + | 32540:13724-13743 | None:intergenic | 60.0% | |
| GGATGAGCCTTCGTCGTTGC+AGG | - | 32540:13714-13733 | MS.gene066342:CDS | 60.0% | |
| TACCACGTCGGACGCTTACC+CGG | - | 32540:13524-13543 | MS.gene066342:CDS | 60.0% | |
| TGGGTGCTAGGTTTGACCGG+CGG | + | 32540:13499-13518 | None:intergenic | 60.0% | |
| ! | TTTGGCGGCGGTAACGGTGT+CGG | + | 32540:13380-13399 | None:intergenic | 60.0% |
| !! | CGGCGATGATAACGGAGGTG+AGG | - | 32540:13576-13595 | MS.gene066342:intron | 60.0% |
| !! | GGCGGTGACGGTATTTTCGG+TGG | - | 32540:13616-13635 | MS.gene066342:intron | 60.0% |
| !!! | GGTGGTGGTGGTGGTTTTGG+AGG | - | 32540:13634-13653 | MS.gene066342:intron | 60.0% |
| !!! | GGTGGTGGTGGTTTTGGAGG+TGG | - | 32540:13637-13656 | MS.gene066342:intron | 60.0% |
| !!! | GGTGGTGGTTTTGGAGGTGG+CGG | - | 32540:13640-13659 | MS.gene066342:intron | 60.0% |
| GTGCTAGGTTTGACCGGCGG+CGG | + | 32540:13496-13515 | None:intergenic | 65.0% | |
| !!! | GGTGGTTTTGGAGGTGGCGG+CGG | - | 32540:13643-13662 | MS.gene066342:intron | 65.0% |
| CACGTCGGACGCTTACCCGG+AGG | - | 32540:13527-13546 | MS.gene066342:CDS | 70.0% | |
| CTCCGGGTAAGCGTCCGACG+TGG | + | 32540:13529-13548 | None:intergenic | 70.0% | |
| ! | TTGGAGGTGGCGGCGGTGGT+GGG | - | 32540:13650-13669 | MS.gene066342:intron | 70.0% |
| ! | TTTGGAGGTGGCGGCGGTGG+TGG | - | 32540:13649-13668 | MS.gene066342:intron | 70.0% |
| !!! | GGTTTTGGAGGTGGCGGCGG+TGG | - | 32540:13646-13665 | MS.gene066342:intron | 70.0% |
| GGCGGAGCCACCGCATCGCA+AGG | + | 32540:13478-13497 | None:intergenic | 75.0% | |
| ! | TGGAGGTGGCGGCGGTGGTG+GGG | - | 32540:13651-13670 | MS.gene066342:intron | 75.0% |
| !! | GCGGCGGTGGTGGGGGTGAT+TGG | - | 32540:13659-13678 | MS.gene066342:CDS | 75.0% |
| ! | CGATGCGGTGGCTCCGCCGC+CGG | - | 32540:13480-13499 | MS.gene066342:CDS | 80.0% |
| !! | GGAGGTGGCGGCGGTGGTGG+GGG | - | 32540:13652-13671 | MS.gene066342:intron | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32540 | gene | 13364 | 13894 | 13364 | ID=MS.gene066342 |
| 32540 | mRNA | 13364 | 13894 | 13364 | ID=MS.gene066342.t1;Parent=MS.gene066342 |
| 32540 | exon | 13659 | 13894 | 13659 | ID=MS.gene066342.t1.exon1;Parent=MS.gene066342.t1 |
| 32540 | CDS | 13659 | 13894 | 13659 | ID=cds.MS.gene066342.t1;Parent=MS.gene066342.t1 |
| 32540 | exon | 13364 | 13565 | 13364 | ID=MS.gene066342.t1.exon2;Parent=MS.gene066342.t1 |
| 32540 | CDS | 13364 | 13565 | 13364 | ID=cds.MS.gene066342.t1;Parent=MS.gene066342.t1 |
| Gene Sequence |
| Protein sequence |
