Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054536.t1 | ACJ83936.1 | 93.1 | 159 | 8 | 2 | 1 | 156 | 1 | 159 | 1.40E-77 | 298.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054536.t1 | Q9SAG8 | 45.1 | 133 | 70 | 2 | 23 | 155 | 33 | 162 | 1.3e-23 | 110.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054536.t1 | B7FGQ7 | 93.1 | 159 | 8 | 2 | 1 | 156 | 1 | 159 | 9.9e-78 | 298.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050288 | MS.gene054536 | 0.819371 | 1.20E-52 | -1.69E-46 |
| MS.gene053189 | MS.gene054536 | 0.887276 | 1.64E-72 | -1.69E-46 |
| MS.gene054536 | MS.gene05469 | 0.819147 | 1.35E-52 | -1.69E-46 |
| MS.gene054536 | MS.gene057999 | 0.812402 | 4.32E-51 | -1.69E-46 |
| MS.gene054536 | MS.gene066342 | 0.80963 | 1.72E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene068166 | 0.80034 | 1.51E-48 | -1.69E-46 |
| MS.gene054536 | MS.gene068565 | 0.820239 | 7.62E-53 | -1.69E-46 |
| MS.gene054536 | MS.gene070011 | 0.804979 | 1.67E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene07339 | 0.806687 | 7.29E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene07552 | 0.803147 | 4.01E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene20064 | 0.801739 | 7.81E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene21659 | 0.806303 | 8.78E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene22314 | 0.812576 | 3.95E-51 | -1.69E-46 |
| MS.gene054536 | MS.gene22433 | 0.8044 | 2.20E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene23152 | -0.802079 | 6.65E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene24413 | 0.803357 | 3.62E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene34115 | 0.800938 | 1.14E-48 | -1.69E-46 |
| MS.gene054536 | MS.gene35750 | 0.803599 | 3.23E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene39139 | 0.80238 | 5.77E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene39317 | 0.817078 | 3.97E-52 | -1.69E-46 |
| MS.gene054536 | MS.gene41775 | 0.823099 | 1.66E-53 | -1.69E-46 |
| MS.gene054536 | MS.gene46301 | 0.834456 | 2.97E-56 | -1.69E-46 |
| MS.gene054536 | MS.gene46355 | 0.806442 | 8.21E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene51920 | 0.808775 | 2.62E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene52973 | 0.806721 | 7.17E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene58467 | 0.822556 | 2.22E-53 | -1.69E-46 |
| MS.gene054536 | MS.gene61254 | 0.800064 | 1.72E-48 | -1.69E-46 |
| MS.gene054536 | MS.gene66408 | 0.84535 | 4.29E-59 | -1.69E-46 |
| MS.gene054536 | MS.gene67136 | 0.807733 | 4.38E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene73270 | 0.821506 | 3.89E-53 | -1.69E-46 |
| MS.gene054536 | MS.gene74854 | 0.805746 | 1.15E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene82350 | 0.804566 | 2.03E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene82352 | 0.800554 | 1.37E-48 | -1.69E-46 |
| MS.gene054536 | MS.gene82976 | 0.804133 | 2.50E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene86392 | 0.807545 | 4.80E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene87924 | 0.847372 | 1.21E-59 | -1.69E-46 |
| MS.gene054536 | MS.gene90077 | 0.803729 | 3.03E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene93983 | 0.814554 | 1.45E-51 | -1.69E-46 |
| MS.gene054536 | MS.gene95469 | 0.814296 | 1.66E-51 | -1.69E-46 |
| MS.gene054536 | MS.gene96209 | 0.808353 | 3.23E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene99465 | 0.805214 | 1.49E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054536.t1 | MTR_4g007300 | 92.547 | 161 | 7 | 2 | 1 | 156 | 1 | 161 | 8.35e-107 | 301 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054536.t1 | AT1G80920 | 62.727 | 110 | 40 | 1 | 46 | 155 | 54 | 162 | 6.80e-48 | 152 |
| MS.gene054536.t1 | AT5G59610 | 29.167 | 144 | 86 | 3 | 11 | 144 | 43 | 180 | 2.53e-13 | 65.9 |
| MS.gene054536.t1 | AT5G59610 | 29.167 | 144 | 86 | 3 | 11 | 144 | 43 | 180 | 2.53e-13 | 65.9 |
Find 37 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACTGCTGTTGGTGTTAT+TGG | 0.156544 | 4.1:+87907580 | MS.gene054536:CDS |
| ATGATCCAGATTGGGGCTAT+TGG | 0.284504 | 4.1:+87908608 | MS.gene054536:CDS |
| TGAATTCTCAAAGTCTTATA+AGG | 0.285846 | 4.1:-87907712 | None:intergenic |
| AAAATAAGATGAATAAAGTT+AGG | 0.311546 | 4.1:+87907650 | MS.gene054536:CDS |
| GTAATGGATCTTGTGTTTCA+TGG | 0.354259 | 4.1:+87907605 | MS.gene054536:CDS |
| TCTCTTCCTTTGCACACATC+TGG | 0.358215 | 4.1:-87907953 | None:intergenic |
| GAGAATGATGAGTCATTTAG+AGG | 0.362832 | 4.1:+87908580 | MS.gene054536:CDS |
| TGATCCAGATTGGGGCTATT+GGG | 0.375755 | 4.1:+87908609 | MS.gene054536:CDS |
| GTTGGTGTTATTGGTGGTAA+TGG | 0.380315 | 4.1:+87907589 | MS.gene054536:CDS |
| TGTTTCATGGATTGGAAGAA+AGG | 0.400791 | 4.1:+87907618 | MS.gene054536:CDS |
| TCTTCCCAATAGCCCCAATC+TGG | 0.418268 | 4.1:-87908613 | None:intergenic |
| GGGCTATTGGGAAGAATGGA+TGG | 0.423463 | 4.1:+87908621 | MS.gene054536:CDS |
| GAGGAATGAATGATCCAGAT+TGG | 0.426354 | 4.1:+87908599 | MS.gene054536:CDS |
| AGGAATGAATGATCCAGATT+GGG | 0.438090 | 4.1:+87908600 | MS.gene054536:CDS |
| ATCATTCTCTTCATTATATG+TGG | 0.460927 | 4.1:-87908566 | None:intergenic |
| GGATCTTGTGTTTCATGGAT+TGG | 0.460943 | 4.1:+87907610 | MS.gene054536:CDS |
| ATTGGGGCTATTGGGAAGAA+TGG | 0.464008 | 4.1:+87908617 | MS.gene054536:CDS |
| TTCTGAATCTGATGTTAGAA+AGG | 0.473384 | 4.1:+87907744 | MS.gene054536:CDS |
| AAATAAGATGAATAAAGTTA+GGG | 0.476392 | 4.1:+87907651 | MS.gene054536:CDS |
| ATCTAAATCTTCTTCTGTGA+TGG | 0.478827 | 4.1:+87907687 | MS.gene054536:CDS |
| TTGGGAAGAATGGATGGGTT+GGG | 0.486704 | 4.1:+87908627 | MS.gene054536:CDS |
| ATTGGGAAGAATGGATGGGT+TGG | 0.503999 | 4.1:+87908626 | MS.gene054536:CDS |
| TTCCTTTGCACACATCTGGA+TGG | 0.515608 | 4.1:-87907949 | None:intergenic |
| ACTGCTGTTGGTGTTATTGG+TGG | 0.520448 | 4.1:+87907583 | MS.gene054536:CDS |
| GGCTATTGGGAAGAATGGAT+GGG | 0.528910 | 4.1:+87908622 | MS.gene054536:CDS |
| GTGTGCAAAGGAAGAGATTG+TGG | 0.529500 | 4.1:+87907959 | MS.gene054536:CDS |
| GAAGAATGGATGGGTTGGGA+AGG | 0.548816 | 4.1:+87908631 | MS.gene054536:CDS |
| TGGATGGGTTGGGAAGGAGC+AGG | 0.559751 | 4.1:+87908637 | MS.gene054536:CDS |
| TGTGCAAAGGAAGAGATTGT+GGG | 0.575497 | 4.1:+87907960 | MS.gene054536:CDS |
| GCAGTTTCACTTAATCAATG+AGG | 0.585631 | 4.1:+87907985 | MS.gene054536:CDS |
| ATGGCTGCTACTACTGCTGT+TGG | 0.598684 | 4.1:+87907571 | MS.gene054536:CDS |
| TACCATCCAGATGTGTGCAA+AGG | 0.620033 | 4.1:+87907947 | MS.gene054536:CDS |
| TAATATGAGAAGAGTAGTCA+CGG | 0.620194 | 4.1:-87908663 | None:intergenic |
| GGAATGAATGATCCAGATTG+GGG | 0.639140 | 4.1:+87908601 | MS.gene054536:CDS |
| ACATCAGATTCAGAAGCACC+AGG | 0.654245 | 4.1:-87907736 | None:intergenic |
| AAGACTTTGAGAATTCAACC+TGG | 0.677099 | 4.1:+87907718 | MS.gene054536:CDS |
| GTGCAAAGGAAGAGATTGTG+GGG | 0.740567 | 4.1:+87907961 | MS.gene054536:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTATTGTTATAATTGTT+TGG | + | chr4.1:87908069-87908088 | MS.gene054536:intron | 10.0% |
| !! | ATGTATTAATATTGAATATT+TGG | + | chr4.1:87907905-87907924 | MS.gene054536:intron | 10.0% |
| !! | ATTTATTGTTATAATTGTTT+GGG | + | chr4.1:87908070-87908089 | MS.gene054536:intron | 10.0% |
| !! | TGTATTAATATTGAATATTT+GGG | + | chr4.1:87907906-87907925 | MS.gene054536:intron | 10.0% |
| !!! | TTTTAGATCATAAAAATATA+TGG | + | chr4.1:87908104-87908123 | MS.gene054536:intron | 10.0% |
| !! | AAAATAAGATGAATAAAGTT+AGG | + | chr4.1:87907650-87907669 | MS.gene054536:CDS | 15.0% |
| !! | AAATAAGATGAATAAAGTTA+GGG | + | chr4.1:87907651-87907670 | MS.gene054536:CDS | 15.0% |
| !! | CATAAATTTCAATTAGATAA+TGG | - | chr4.1:87908473-87908492 | None:intergenic | 15.0% |
| !!! | AATTTGTTTTTATCTTCTTA+TGG | + | chr4.1:87908405-87908424 | MS.gene054536:intron | 15.0% |
| !!! | ATTTGTTTTTATCTTCTTAT+GGG | + | chr4.1:87908406-87908425 | MS.gene054536:intron | 15.0% |
| !!! | TTTTTTATTGTTTATGGTTT+TGG | + | chr4.1:87908272-87908291 | MS.gene054536:intron | 15.0% |
| !! | AACTATGCAATGAAAATTAA+AGG | - | chr4.1:87908049-87908068 | None:intergenic | 20.0% |
| !! | ACAATAAAAAAAACTGGATT+TGG | - | chr4.1:87908263-87908282 | None:intergenic | 20.0% |
| !! | CCATAAACAATAAAAAAAAC+TGG | - | chr4.1:87908269-87908288 | None:intergenic | 20.0% |
| !! | GTTTATCAAGTTATAAATCT+TGG | - | chr4.1:87908309-87908328 | None:intergenic | 20.0% |
| !! | TCAAACAAATCATTAAACTA+TGG | - | chr4.1:87908433-87908452 | None:intergenic | 20.0% |
| !!! | AATTTATGTGTTGCTTATAT+TGG | + | chr4.1:87908485-87908504 | MS.gene054536:intron | 20.0% |
| !!! | ACTAATTTGATTGATGATTT+TGG | + | chr4.1:87907816-87907835 | MS.gene054536:intron | 20.0% |
| !!! | CCAGTTTTTTTTATTGTTTA+TGG | + | chr4.1:87908266-87908285 | MS.gene054536:intron | 20.0% |
| !!! | GTTGTTTTATTTCAAATCAA+TGG | - | chr4.1:87908205-87908224 | None:intergenic | 20.0% |
| ! | ATCATTCTCTTCATTATATG+TGG | - | chr4.1:87908569-87908588 | None:intergenic | 25.0% |
| ! | ATTGGAGATATAAGATCAAT+GGG | + | chr4.1:87908139-87908158 | MS.gene054536:intron | 25.0% |
| ! | CATTATACATGAAGAAAGAA+AGG | - | chr4.1:87907888-87907907 | None:intergenic | 25.0% |
| !! | ATATGGATATTGATTGACAT+TGG | + | chr4.1:87908121-87908140 | MS.gene054536:intron | 25.0% |
| !! | TGAATTCTCAAAGTCTTATA+AGG | - | chr4.1:87907715-87907734 | None:intergenic | 25.0% |
| !! | TGGATTTGTAATCTTGATTT+TGG | + | chr4.1:87907836-87907855 | MS.gene054536:intron | 25.0% |
| !! | TTTCACATTTTCTCTCAAAT+TGG | - | chr4.1:87908530-87908549 | None:intergenic | 25.0% |
| ATCTAAATCTTCTTCTGTGA+TGG | + | chr4.1:87907687-87907706 | MS.gene054536:CDS | 30.0% | |
| CATTGGAGATATAAGATCAA+TGG | + | chr4.1:87908138-87908157 | MS.gene054536:intron | 30.0% | |
| TAATATGAGAAGAGTAGTCA+CGG | - | chr4.1:87908666-87908685 | None:intergenic | 30.0% | |
| TGTGACACAATTATTGATCT+TGG | - | chr4.1:87908227-87908246 | None:intergenic | 30.0% | |
| TTCTGAATCTGATGTTAGAA+AGG | + | chr4.1:87907744-87907763 | MS.gene054536:CDS | 30.0% | |
| ! | GGATAGCTTAGAGAAATTTT+AGG | - | chr4.1:87908028-87908047 | None:intergenic | 30.0% |
| AGGAATGAATGATCCAGATT+GGG | + | chr4.1:87908600-87908619 | MS.gene054536:CDS | 35.0% | |
| CTTCTCATCTATGGTTTCAT+TGG | - | chr4.1:87908375-87908394 | None:intergenic | 35.0% | |
| GAGAATGATGAGTCATTTAG+AGG | + | chr4.1:87908580-87908599 | MS.gene054536:CDS | 35.0% | |
| GCAGTTTCACTTAATCAATG+AGG | + | chr4.1:87907985-87908004 | MS.gene054536:CDS | 35.0% | |
| ! | AAGACTTTGAGAATTCAACC+TGG | + | chr4.1:87907718-87907737 | MS.gene054536:CDS | 35.0% |
| !! | CTTTTGTTGCTTCTCATCTA+TGG | - | chr4.1:87908384-87908403 | None:intergenic | 35.0% |
| !! | GTAATGGATCTTGTGTTTCA+TGG | + | chr4.1:87907605-87907624 | MS.gene054536:CDS | 35.0% |
| !! | TGTTTCATGGATTGGAAGAA+AGG | + | chr4.1:87907618-87907637 | MS.gene054536:CDS | 35.0% |
| !! | TTTTAGACAACTTGCTTTGC+AGG | + | chr4.1:87907768-87907787 | MS.gene054536:CDS | 35.0% |
| GAGGAATGAATGATCCAGAT+TGG | + | chr4.1:87908599-87908618 | MS.gene054536:CDS | 40.0% | |
| GGAATGAATGATCCAGATTG+GGG | + | chr4.1:87908601-87908620 | MS.gene054536:CDS | 40.0% | |
| GGATCTTGTGTTTCATGGAT+TGG | + | chr4.1:87907610-87907629 | MS.gene054536:CDS | 40.0% | |
| TGTGCAAAGGAAGAGATTGT+GGG | + | chr4.1:87907960-87907979 | MS.gene054536:CDS | 40.0% | |
| !! | ACTACTGCTGTTGGTGTTAT+TGG | + | chr4.1:87907580-87907599 | MS.gene054536:CDS | 40.0% |
| !! | GTTGGTGTTATTGGTGGTAA+TGG | + | chr4.1:87907589-87907608 | MS.gene054536:CDS | 40.0% |
| ATGATCCAGATTGGGGCTAT+TGG | + | chr4.1:87908608-87908627 | MS.gene054536:CDS | 45.0% | |
| ATTGGGAAGAATGGATGGGT+TGG | + | chr4.1:87908626-87908645 | MS.gene054536:CDS | 45.0% | |
| ATTGGGGCTATTGGGAAGAA+TGG | + | chr4.1:87908617-87908636 | MS.gene054536:CDS | 45.0% | |
| GGCTATTGGGAAGAATGGAT+GGG | + | chr4.1:87908622-87908641 | MS.gene054536:CDS | 45.0% | |
| GTGCAAAGGAAGAGATTGTG+GGG | + | chr4.1:87907961-87907980 | MS.gene054536:CDS | 45.0% | |
| GTGTGCAAAGGAAGAGATTG+TGG | + | chr4.1:87907959-87907978 | MS.gene054536:CDS | 45.0% | |
| TACCATCCAGATGTGTGCAA+AGG | + | chr4.1:87907947-87907966 | MS.gene054536:CDS | 45.0% | |
| TCTCTTCCTTTGCACACATC+TGG | - | chr4.1:87907956-87907975 | None:intergenic | 45.0% | |
| TGATCCAGATTGGGGCTATT+GGG | + | chr4.1:87908609-87908628 | MS.gene054536:CDS | 45.0% | |
| TTCCTTTGCACACATCTGGA+TGG | - | chr4.1:87907952-87907971 | None:intergenic | 45.0% | |
| ! | TTGGGAAGAATGGATGGGTT+GGG | + | chr4.1:87908627-87908646 | MS.gene054536:CDS | 45.0% |
| !! | ACATCAGATTCAGAAGCACC+AGG | - | chr4.1:87907739-87907758 | None:intergenic | 45.0% |
| !! | ACTGCTGTTGGTGTTATTGG+TGG | + | chr4.1:87907583-87907602 | MS.gene054536:CDS | 45.0% |
| ATGGCTGCTACTACTGCTGT+TGG | + | chr4.1:87907571-87907590 | MS.gene054536:CDS | 50.0% | |
| GGGCTATTGGGAAGAATGGA+TGG | + | chr4.1:87908621-87908640 | MS.gene054536:CDS | 50.0% | |
| TCTTCCCAATAGCCCCAATC+TGG | - | chr4.1:87908616-87908635 | None:intergenic | 50.0% | |
| !! | GAAGAATGGATGGGTTGGGA+AGG | + | chr4.1:87908631-87908650 | MS.gene054536:CDS | 50.0% |
| !! | TGGATGGGTTGGGAAGGAGC+AGG | + | chr4.1:87908637-87908656 | MS.gene054536:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 87907571 | 87908699 | 87907571 | ID=MS.gene054536 |
| chr4.1 | mRNA | 87907571 | 87908699 | 87907571 | ID=MS.gene054536.t1;Parent=MS.gene054536 |
| chr4.1 | exon | 87907571 | 87907789 | 87907571 | ID=MS.gene054536.t1.exon1;Parent=MS.gene054536.t1 |
| chr4.1 | CDS | 87907571 | 87907789 | 87907571 | ID=cds.MS.gene054536.t1;Parent=MS.gene054536.t1 |
| chr4.1 | exon | 87907947 | 87908015 | 87907947 | ID=MS.gene054536.t1.exon2;Parent=MS.gene054536.t1 |
| chr4.1 | CDS | 87907947 | 87908015 | 87907947 | ID=cds.MS.gene054536.t1;Parent=MS.gene054536.t1 |
| chr4.1 | exon | 87908517 | 87908699 | 87908517 | ID=MS.gene054536.t1.exon3;Parent=MS.gene054536.t1 |
| chr4.1 | CDS | 87908517 | 87908699 | 87908517 | ID=cds.MS.gene054536.t1;Parent=MS.gene054536.t1 |
| Gene Sequence |
| Protein sequence |
