Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06657.t1 | XP_003602304.1 | 98.8 | 162 | 2 | 0 | 1 | 162 | 1 | 162 | 7.50E-87 | 329.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06657.t1 | G7J857 | 98.8 | 162 | 2 | 0 | 1 | 162 | 1 | 162 | 5.4e-87 | 329.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene06657 | 0.800227 | 1.59E-48 | -1.69E-46 |
| MS.gene049378 | MS.gene06657 | 0.821077 | 4.89E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene06657 | 0.810255 | 1.26E-50 | -1.69E-46 |
| MS.gene052389 | MS.gene06657 | 0.801096 | 1.06E-48 | -1.69E-46 |
| MS.gene052464 | MS.gene06657 | 0.833732 | 4.50E-56 | -1.69E-46 |
| MS.gene053584 | MS.gene06657 | 0.803718 | 3.05E-49 | -1.69E-46 |
| MS.gene054093 | MS.gene06657 | 0.800146 | 1.65E-48 | -1.69E-46 |
| MS.gene054930 | MS.gene06657 | 0.836687 | 8.08E-57 | -1.69E-46 |
| MS.gene055701 | MS.gene06657 | 0.835132 | 2.00E-56 | -1.69E-46 |
| MS.gene056506 | MS.gene06657 | 0.804351 | 2.25E-49 | -1.69E-46 |
| MS.gene057194 | MS.gene06657 | 0.955596 | 2.10E-113 | -1.69E-46 |
| MS.gene05761 | MS.gene06657 | 0.84604 | 2.79E-59 | -1.69E-46 |
| MS.gene060011 | MS.gene06657 | 0.814736 | 1.32E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06657.t1 | MTR_3g092020 | 98.765 | 162 | 2 | 0 | 1 | 162 | 1 | 162 | 6.52e-119 | 332 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCCCATCTCTCCTTAAAA+AGG | 0.245866 | 3.3:-76836953 | None:intergenic |
| CAAAATGGAAACCTTACCTT+TGG | 0.392712 | 3.3:+76835795 | None:intergenic |
| CTCCTCTTGGTTCAAAATCA+TGG | 0.393681 | 3.3:+76836997 | MS.gene06657:CDS |
| GAGATCATGTCGAGGAATCT+TGG | 0.402090 | 3.3:+76837651 | MS.gene06657:CDS |
| TGGCCTCGGACAATGCTCTA+TGG | 0.432233 | 3.3:+76836925 | MS.gene06657:CDS |
| TTATATCACACGGCTGCGTT+TGG | 0.432650 | 3.3:-76837722 | None:intergenic |
| CATACAGATTTCCAAAGGTA+AGG | 0.450513 | 3.3:-76835806 | None:intergenic |
| GGTGAGGTACAACGATATCT+AGG | 0.477811 | 3.3:+76837544 | MS.gene06657:CDS |
| AGTTCAGAAACAGACTTCAC+TGG | 0.488792 | 3.3:+76837607 | MS.gene06657:CDS |
| GAAGTGGAAGCTCATGGCCT+CGG | 0.508465 | 3.3:+76836911 | MS.gene06657:CDS |
| GGTTTGAAGATTGTTAGAGA+AGG | 0.530829 | 3.3:+76837496 | MS.gene06657:CDS |
| TTGTTGAGAAACAGCAAGAT+GGG | 0.537051 | 3.3:-76835854 | None:intergenic |
| ATCATGGAAAGAAGTGTATG+AGG | 0.545115 | 3.3:+76837013 | MS.gene06657:CDS |
| CATCTTGCTGTTTCTCAACA+AGG | 0.545411 | 3.3:+76835856 | MS.gene06657:CDS |
| CAAGATCGATGCGATCGAGT+TGG | 0.561970 | 3.3:+76837038 | MS.gene06657:CDS |
| CTGTAGGAAGTGGAAGCTCA+TGG | 0.592096 | 3.3:+76836905 | MS.gene06657:CDS |
| CTTGTTGAGAAACAGCAAGA+TGG | 0.595023 | 3.3:-76835855 | None:intergenic |
| ATCTTCATACAGATTTCCAA+AGG | 0.614774 | 3.3:-76835811 | None:intergenic |
| GAGAAACAGCAAGATGGGTG+TGG | 0.636024 | 3.3:-76835849 | None:intergenic |
| GATATCGTTGTACCTCACCT+TGG | 0.640965 | 3.3:-76837540 | None:intergenic |
| CTATCTTGTTCACCAAGGTG+AGG | 0.666547 | 3.3:+76837528 | MS.gene06657:CDS |
| GTAGAGATGGTTATATCACA+CGG | 0.677630 | 3.3:-76837732 | None:intergenic |
| TGACCATAGAGCATTGTCCG+AGG | 0.683112 | 3.3:-76836928 | None:intergenic |
| GCTGAAGAGAGATCATGTCG+AGG | 0.706201 | 3.3:+76837643 | MS.gene06657:CDS |
| GACTACTATCTTGTTCACCA+AGG | 0.711703 | 3.3:+76837523 | MS.gene06657:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTAATTAATAATAATTT+TGG | + | chr3.3:76837226-76837245 | MS.gene06657:intron | 0.0% |
| !!! | AAAAAATTAACAAATTGTTT+TGG | - | chr3.3:76836692-76836711 | None:intergenic | 10.0% |
| !!! | TTTTAGAAGAAAAAAAATAT+GGG | - | chr3.3:76837255-76837274 | None:intergenic | 10.0% |
| !! | AATTGAATCAATCATAATAT+TGG | + | chr3.3:76836591-76836610 | MS.gene06657:intron | 15.0% |
| !! | AATTGTATAACAAAATTTGT+TGG | - | chr3.3:76836305-76836324 | None:intergenic | 15.0% |
| !!! | AACAATAAAGATTGAATTTT+AGG | - | chr3.3:76836485-76836504 | None:intergenic | 15.0% |
| !!! | ACAAAATTCTTTTTTTCATT+GGG | + | chr3.3:76837674-76837693 | MS.gene06657:CDS | 15.0% |
| !!! | ATAATTTGTTATGTTGAATA+TGG | - | chr3.3:76837360-76837379 | None:intergenic | 15.0% |
| !!! | GTTTTAGAAGAAAAAAAATA+TGG | - | chr3.3:76837256-76837275 | None:intergenic | 15.0% |
| !!! | TTTTAATTCTTCACAAAATA+GGG | + | chr3.3:76837474-76837493 | MS.gene06657:intron | 15.0% |
| !!! | TTTTTAATTCTTCACAAAAT+AGG | + | chr3.3:76837473-76837492 | MS.gene06657:intron | 15.0% |
| !! | AAAACAATTTGTCAAATTCA+AGG | - | chr3.3:76836880-76836899 | None:intergenic | 20.0% |
| !! | ATCAATCATAATATTGGATT+AGG | + | chr3.3:76836597-76836616 | MS.gene06657:intron | 20.0% |
| !! | CAAGAATATAAAAATGAAGA+TGG | - | chr3.3:76835912-76835931 | None:intergenic | 20.0% |
| !! | TTATTGCTTCAAATTAAACT+AGG | - | chr3.3:76836349-76836368 | None:intergenic | 20.0% |
| !! | TTGACTGTAATGATATAAAA+AGG | - | chr3.3:76836573-76836592 | None:intergenic | 20.0% |
| !! | TTTAATTCTTCACAAAATAG+GGG | + | chr3.3:76837475-76837494 | MS.gene06657:intron | 20.0% |
| !!! | ATTTGAAATTCATAAGTCAA+CGG | - | chr3.3:76835978-76835997 | None:intergenic | 20.0% |
| !!! | CAAAATTCTTTTTTTCATTG+GGG | + | chr3.3:76837675-76837694 | MS.gene06657:CDS | 20.0% |
| !!! | CTGAGTTTATTACATTATTT+TGG | + | chr3.3:76836849-76836868 | MS.gene06657:intron | 20.0% |
| !!! | GACAAAATTCTTTTTTTCAT+TGG | + | chr3.3:76837673-76837692 | MS.gene06657:CDS | 20.0% |
| !!! | TCAATCTTTATTGTTTTGAT+CGG | + | chr3.3:76836490-76836509 | MS.gene06657:intron | 20.0% |
| !!! | TTGTCAAATATCTTTTTAGA+GGG | - | chr3.3:76836799-76836818 | None:intergenic | 20.0% |
| ! | AAAATCTCCTTCAATAATCA+TGG | - | chr3.3:76837146-76837165 | None:intergenic | 25.0% |
| ! | AACCTATGTGTAACATTAAA+AGG | - | chr3.3:76836723-76836742 | None:intergenic | 25.0% |
| ! | AATAACAATATTTCAACCGA+TGG | - | chr3.3:76836013-76836032 | None:intergenic | 25.0% |
| ! | ATCTAGGTTCAAGAAAAAAT+CGG | + | chr3.3:76837560-76837579 | MS.gene06657:CDS | 25.0% |
| ! | TGTTGGATATCATATGATAT+AGG | - | chr3.3:76836288-76836307 | None:intergenic | 25.0% |
| ! | TTAAATGCCATGATTATTGA+AGG | + | chr3.3:76837136-76837155 | MS.gene06657:intron | 25.0% |
| ! | TTATTAGATCTGAATCTGTA+TGG | + | chr3.3:76836028-76836047 | MS.gene06657:intron | 25.0% |
| !! | ACTGTGATTTTATGATACAT+TGG | + | chr3.3:76836526-76836545 | MS.gene06657:intron | 25.0% |
| !! | CTTGTCAAATATCTTTTTAG+AGG | - | chr3.3:76836800-76836819 | None:intergenic | 25.0% |
| !! | TTCCTTTTAATGTTACACAT+AGG | + | chr3.3:76836718-76836737 | MS.gene06657:intron | 25.0% |
| !! | TTCTTTGAAAAGAAACATAG+CGG | - | chr3.3:76836378-76836397 | None:intergenic | 25.0% |
| AAATATGAATCAAGTCCATG+TGG | - | chr3.3:76836449-76836468 | None:intergenic | 30.0% | |
| ATCTTCATACAGATTTCCAA+AGG | - | chr3.3:76835814-76835833 | None:intergenic | 30.0% | |
| TCTTTATCAGAAAACAGTGT+TGG | + | chr3.3:76836159-76836178 | MS.gene06657:intron | 30.0% | |
| TTATTGAACTCTGACACAAA+GGG | + | chr3.3:76837443-76837462 | MS.gene06657:intron | 30.0% | |
| TTTCTGCACACTTATAACAT+TGG | + | chr3.3:76837290-76837309 | MS.gene06657:intron | 30.0% | |
| ! | TCTATGGTCAAACCTTTTTA+AGG | + | chr3.3:76836941-76836960 | MS.gene06657:CDS | 30.0% |
| !! | AAACCTTTTTAAGGAGAGAT+GGG | + | chr3.3:76836950-76836969 | MS.gene06657:CDS | 30.0% |
| !! | CAAATTGTTTTACAGTCTGT+AGG | + | chr3.3:76836889-76836908 | MS.gene06657:intron | 30.0% |
| !!! | TCTTTTTTTCATTGGGGATT+TGG | + | chr3.3:76837681-76837700 | MS.gene06657:CDS | 30.0% |
| !!! | TTTTGTTTTCTGCGAAGAAA+TGG | + | chr3.3:76836228-76836247 | MS.gene06657:intron | 30.0% |
| AAATCAGTTGCATGTTCCAT+CGG | + | chr3.3:76835994-76836013 | MS.gene06657:intron | 35.0% | |
| ATAATTGCAAGCGTGTGATT+AGG | - | chr3.3:76837180-76837199 | None:intergenic | 35.0% | |
| ATAGGTTAAAACCGACGATT+TGG | + | chr3.3:76836736-76836755 | MS.gene06657:intron | 35.0% | |
| ATTATTCTTGTCCCTTGAGA+TGG | + | chr3.3:76837195-76837214 | MS.gene06657:intron | 35.0% | |
| ATTTGGCAACTGAGTGTTAA+CGG | + | chr3.3:76836760-76836779 | MS.gene06657:intron | 35.0% | |
| CACACTTATAACATTGGTGT+TGG | + | chr3.3:76837296-76837315 | MS.gene06657:intron | 35.0% | |
| CATACAGATTTCCAAAGGTA+AGG | - | chr3.3:76835809-76835828 | None:intergenic | 35.0% | |
| CTAAAGCAAATCCAAGTAAG+AGG | - | chr3.3:76837325-76837344 | None:intergenic | 35.0% | |
| CTTATTGAACTCTGACACAA+AGG | + | chr3.3:76837442-76837461 | MS.gene06657:intron | 35.0% | |
| GGATTAACAATATGTGACAG+AGG | - | chr3.3:76836267-76836286 | None:intergenic | 35.0% | |
| GGTCAAATTCCAGATCATAA+TGG | - | chr3.3:76836418-76836437 | None:intergenic | 35.0% | |
| GGTTTGAAGATTGTTAGAGA+AGG | + | chr3.3:76837496-76837515 | MS.gene06657:CDS | 35.0% | |
| TTGTTGAGAAACAGCAAGAT+GGG | - | chr3.3:76835857-76835876 | None:intergenic | 35.0% | |
| TTTGACCAAAATTTGCCACA+TGG | + | chr3.3:76836431-76836450 | MS.gene06657:intron | 35.0% | |
| ! | ATAATTTTCACGCCATCTCA+AGG | - | chr3.3:76837210-76837229 | None:intergenic | 35.0% |
| ! | ATCATGGAAAGAAGTGTATG+AGG | + | chr3.3:76837013-76837032 | MS.gene06657:CDS | 35.0% |
| ! | TAATTTTCACGCCATCTCAA+GGG | - | chr3.3:76837209-76837228 | None:intergenic | 35.0% |
| ! | TTATCAGAAAACAGTGTTGG+TGG | + | chr3.3:76836162-76836181 | MS.gene06657:intron | 35.0% |
| ! | TTCCATGATTTTGAACCAAG+AGG | - | chr3.3:76837002-76837021 | None:intergenic | 35.0% |
| !! | AAAACCGACGATTTGGTATT+TGG | + | chr3.3:76836743-76836762 | MS.gene06657:intron | 35.0% |
| !! | AACCTTTTTAAGGAGAGATG+GGG | + | chr3.3:76836951-76836970 | MS.gene06657:CDS | 35.0% |
| !! | CAAACCTTTTTAAGGAGAGA+TGG | + | chr3.3:76836949-76836968 | MS.gene06657:CDS | 35.0% |
| AAAAATCGGCGACAAGTATC+TGG | + | chr3.3:76837574-76837593 | MS.gene06657:CDS | 40.0% | |
| AGTTCAGAAACAGACTTCAC+TGG | + | chr3.3:76837607-76837626 | MS.gene06657:CDS | 40.0% | |
| CTCCTCTTGGTTCAAAATCA+TGG | + | chr3.3:76836997-76837016 | MS.gene06657:CDS | 40.0% | |
| CTTGTTGAGAAACAGCAAGA+TGG | - | chr3.3:76835858-76835877 | None:intergenic | 40.0% | |
| GACTACTATCTTGTTCACCA+AGG | + | chr3.3:76837523-76837542 | MS.gene06657:CDS | 40.0% | |
| GCAAATCAGCAAGACATATG+CGG | - | chr3.3:76836670-76836689 | None:intergenic | 40.0% | |
| GGTTCACAGAACACAGTTAT+TGG | - | chr3.3:76836328-76836347 | None:intergenic | 40.0% | |
| GTTGCCAAATACCAAATCGT+CGG | - | chr3.3:76836750-76836769 | None:intergenic | 40.0% | |
| TTACTCTGCTGAGAGTAGTA+CGG | + | chr3.3:76837078-76837097 | MS.gene06657:intron | 40.0% | |
| ! | CAAGTCCATGTGGCAAATTT+TGG | - | chr3.3:76836439-76836458 | None:intergenic | 40.0% |
| ! | GTGTTGGTGGAATTGAAAAC+AGG | + | chr3.3:76836175-76836194 | MS.gene06657:intron | 40.0% |
| ! | GTTTTACAGTCTGTAGGAAG+TGG | + | chr3.3:76836895-76836914 | MS.gene06657:intron | 40.0% |
| !! | CATCTTGCTGTTTCTCAACA+AGG | + | chr3.3:76835856-76835875 | MS.gene06657:CDS | 40.0% |
| CCTCAGCTTCCATTATGATC+TGG | + | chr3.3:76836406-76836425 | MS.gene06657:intron | 45.0% | |
| CTATCTTGTTCACCAAGGTG+AGG | + | chr3.3:76837528-76837547 | MS.gene06657:CDS | 45.0% | |
| CTCCCCATCTCTCCTTAAAA+AGG | - | chr3.3:76836956-76836975 | None:intergenic | 45.0% | |
| GAGATCATGTCGAGGAATCT+TGG | + | chr3.3:76837651-76837670 | MS.gene06657:CDS | 45.0% | |
| GATATCGTTGTACCTCACCT+TGG | - | chr3.3:76837543-76837562 | None:intergenic | 45.0% | |
| GGTGAGGTACAACGATATCT+AGG | + | chr3.3:76837544-76837563 | MS.gene06657:CDS | 45.0% | |
| TTATATCACACGGCTGCGTT+TGG | - | chr3.3:76837725-76837744 | None:intergenic | 45.0% | |
| ! | CCAGATCATAATGGAAGCTG+AGG | - | chr3.3:76836409-76836428 | None:intergenic | 45.0% |
| !! | CTTTTTAAGGAGAGATGGGG+AGG | + | chr3.3:76836954-76836973 | MS.gene06657:CDS | 45.0% |
| !!! | GCTGCTTTTTATGCTCCTCT+TGG | + | chr3.3:76836984-76837003 | MS.gene06657:CDS | 45.0% |
| CAAGATCGATGCGATCGAGT+TGG | + | chr3.3:76837038-76837057 | MS.gene06657:CDS | 50.0% | |
| CTGTAGGAAGTGGAAGCTCA+TGG | + | chr3.3:76836905-76836924 | MS.gene06657:CDS | 50.0% | |
| GAGAAACAGCAAGATGGGTG+TGG | - | chr3.3:76835852-76835871 | None:intergenic | 50.0% | |
| GCTGAAGAGAGATCATGTCG+AGG | + | chr3.3:76837643-76837662 | MS.gene06657:CDS | 50.0% | |
| !! | GGTGTTGGCTTCCTCTTACT+TGG | + | chr3.3:76837311-76837330 | MS.gene06657:intron | 50.0% |
| !! | TGACCATAGAGCATTGTCCG+AGG | - | chr3.3:76836931-76836950 | None:intergenic | 50.0% |
| GAAGTGGAAGCTCATGGCCT+CGG | + | chr3.3:76836911-76836930 | MS.gene06657:CDS | 55.0% | |
| TGGCCTCGGACAATGCTCTA+TGG | + | chr3.3:76836925-76836944 | MS.gene06657:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 76835799 | 76837744 | 76835799 | ID=MS.gene06657 |
| chr3.3 | mRNA | 76835799 | 76837744 | 76835799 | ID=MS.gene06657.t1;Parent=MS.gene06657 |
| chr3.3 | exon | 76835799 | 76835877 | 76835799 | ID=MS.gene06657.t1.exon1;Parent=MS.gene06657.t1 |
| chr3.3 | CDS | 76835799 | 76835877 | 76835799 | ID=cds.MS.gene06657.t1;Parent=MS.gene06657.t1 |
| chr3.3 | exon | 76836904 | 76837063 | 76836904 | ID=MS.gene06657.t1.exon2;Parent=MS.gene06657.t1 |
| chr3.3 | CDS | 76836904 | 76837063 | 76836904 | ID=cds.MS.gene06657.t1;Parent=MS.gene06657.t1 |
| chr3.3 | exon | 76837495 | 76837744 | 76837495 | ID=MS.gene06657.t1.exon3;Parent=MS.gene06657.t1 |
| chr3.3 | CDS | 76837495 | 76837744 | 76837495 | ID=cds.MS.gene06657.t1;Parent=MS.gene06657.t1 |
| Gene Sequence |
| Protein sequence |
