AlfalfaGEDB | MS.gene068291

M. sativa cultivar XinJiangDaYe / MS.gene068291

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068291.t1	KEH28826.1	84.7	202	28	1	1	202	1	199	9.10E-90	339.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068291.t1	Q9C891	63.5	167	56	2	36	202	26	187	6.6e-57	221.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068291.t1	A0A072UHF1	84.7	202	28	1	1	202	1	199	6.6e-90	339.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene003512	MS.gene068291	PPI
MS.gene068291	MS.gene33680	PPI
MS.gene068291	MS.gene33420	PPI
MS.gene33424	MS.gene068291	PPI
MS.gene33418	MS.gene068291	PPI
MS.gene048891	MS.gene068291	PPI
MS.gene55764	MS.gene068291	PPI
MS.gene33680	MS.gene068291	PPI
MS.gene068291	MS.gene33426	PPI
MS.gene071329	MS.gene068291	PPI
MS.gene068291	MS.gene06033	PPI
MS.gene98692	MS.gene068291	PPI
MS.gene06035	MS.gene068291	PPI
MS.gene057717	MS.gene068291	PPI
MS.gene068291	MS.gene52084	PPI
MS.gene33677	MS.gene068291	PPI
MS.gene068291	MS.gene068292	PPI
MS.gene068291	MS.gene33681	PPI
MS.gene068291	MS.gene24969	PPI
MS.gene55766	MS.gene068291	PPI
MS.gene024359	MS.gene068291	PPI
MS.gene24971	MS.gene068291	PPI
MS.gene068294	MS.gene068291	PPI
MS.gene068291	MS.gene90969	PPI
MS.gene068291	MS.gene33676	PPI
MS.gene33679	MS.gene068291	PPI
MS.gene24973	MS.gene068291	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068291.t1	MTR_4g013330	93.069	202	11	1	1	202	1	199	3.67e-132	369
MS.gene068291.t1	MTR_4g013345	80.198	202	34	2	1	202	1	196	5.74e-113	320
MS.gene068291.t1	MTR_4g013350	78.218	202	39	2	1	202	1	197	1.06e-111	317
MS.gene068291.t1	MTR_4g013320	78.610	187	37	2	16	202	16	199	5.09e-107	305
MS.gene068291.t1	MTR_4g013335	71.123	187	47	2	16	202	17	196	7.09e-99	285
MS.gene068291.t1	MTR_4g013325	73.267	202	51	2	1	202	1	199	1.22e-90	264
MS.gene068291.t1	MTR_4g013310	68.812	202	58	3	1	202	1	197	5.87e-90	262
MS.gene068291.t1	MTR_7g070390	68.605	172	50	2	32	202	24	192	3.35e-77	230
MS.gene068291.t1	MTR_4g013385	55.670	194	75	3	9	202	8	190	1.17e-72	218
MS.gene068291.t1	MTR_0433s0040	55.670	194	75	3	9	202	8	190	1.17e-72	218
MS.gene068291.t1	MTR_4g013770	60.843	166	62	2	37	202	29	191	2.81e-67	204
MS.gene068291.t1	MTR_1g054525	57.485	167	67	2	36	202	30	192	1.45e-64	197
MS.gene068291.t1	MTR_4g013355	50.515	194	76	5	9	202	6	179	2.01e-60	187
MS.gene068291.t1	MTR_4g073950	52.941	153	71	1	50	202	41	192	1.13e-55	175
MS.gene068291.t1	MTR_7g093870	52.564	156	71	1	50	202	38	193	7.45e-55	174
MS.gene068291.t1	MTR_7g093850	47.917	192	90	5	13	202	11	194	1.47e-54	172
MS.gene068291.t1	MTR_7g093820	47.396	192	91	5	13	202	11	194	3.64e-54	171
MS.gene068291.t1	MTR_7g093830	45.833	192	94	5	13	202	10	193	5.54e-53	168
MS.gene068291.t1	MTR_7g093790	47.024	168	85	3	36	202	27	191	8.42e-53	168
MS.gene068291.t1	MTR_1g056370	49.020	153	74	2	50	201	36	185	2.79e-48	156
MS.gene068291.t1	MTR_3g105640	45.062	162	87	2	42	202	28	188	7.97e-46	150
MS.gene068291.t1	MTR_3g105630	44.654	159	87	1	45	202	31	189	1.51e-44	147
MS.gene068291.t1	MTR_4g078885	45.695	151	78	1	52	202	39	185	3.25e-44	145
MS.gene068291.t1	MTR_3g034030	45.695	151	78	1	52	202	39	185	1.22e-43	144
MS.gene068291.t1	MTR_5g096120	41.667	156	81	3	53	202	38	189	3.72e-39	133
MS.gene068291.t1	MTR_4g122110	34.211	152	90	2	55	202	35	180	1.39e-23	92.8
MS.gene068291.t1	MTR_4g122130	34.247	146	90	2	59	202	39	180	6.17e-23	91.3
MS.gene068291.t1	MTR_4g049550	31.298	131	84	2	49	177	27	153	2.14e-16	73.9
MS.gene068291.t1	MTR_4g049570	28.966	145	101	1	53	197	31	173	2.69e-15	70.9
MS.gene068291.t1	MTR_8g099115	34.559	136	82	4	52	181	34	168	8.68e-14	67.0
MS.gene068291.t1	MTR_8g099135	34.848	132	71	5	60	181	38	164	3.60e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068291.t1	AT1G55210	58.763	194	70	3	9	202	4	187	1.05e-77	231
MS.gene068291.t1	AT1G55210	58.763	194	70	3	9	202	4	187	1.05e-77	231
MS.gene068291.t1	AT1G58170	57.592	191	69	3	12	202	7	185	1.41e-75	225
MS.gene068291.t1	AT5G49040	54.167	192	84	2	11	202	4	191	2.92e-71	214
MS.gene068291.t1	AT3G13650	51.562	192	84	4	11	202	4	186	1.41e-63	195
MS.gene068291.t1	AT5G49030	52.941	187	82	3	11	197	4	184	5.42e-56	191
MS.gene068291.t1	AT3G13662	56.291	151	61	1	39	189	27	172	9.68e-55	172
MS.gene068291.t1	AT1G65870	47.712	153	78	1	49	201	39	189	2.64e-51	164
MS.gene068291.t1	AT3G13660	58.594	128	50	1	75	202	1	125	6.31e-49	155
MS.gene068291.t1	AT2G21100	48.344	151	76	1	51	201	39	187	1.16e-48	157
MS.gene068291.t1	AT2G21100	48.344	151	76	1	51	201	39	187	1.16e-48	157
MS.gene068291.t1	AT1G22900	49.020	153	74	2	49	201	45	193	1.80e-47	154
MS.gene068291.t1	AT5G42500	47.368	152	76	3	50	201	38	185	1.34e-46	152
MS.gene068291.t1	AT5G42510	46.405	153	76	3	50	201	35	182	2.74e-45	148
MS.gene068291.t1	AT4G38700	37.438	203	109	5	4	202	2	190	4.71e-39	132
MS.gene068291.t1	AT2G21110	40.000	175	91	3	33	202	21	186	1.81e-38	131
MS.gene068291.t1	AT3G58090	50.000	110	55	0	92	201	162	271	4.71e-36	127
MS.gene068291.t1	AT4G23690	33.333	138	83	4	48	181	34	166	6.99e-15	70.1
MS.gene068291.t1	AT4G11210	28.814	177	111	5	9	185	5	166	1.94e-14	68.9

Find 48 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGATAGACCGCAAGTTATT+AGG	0.219308	4.4:+86738875	MS.gene068291:CDS
ATGGACAGCCCTTTGACTTT+AGG	0.270088	4.4:+86739043	MS.gene068291:CDS
TCAACCGGCCTTAATGCTTT+TGG	0.320368	4.4:+86739007	MS.gene068291:CDS
CATTGGGTTGATCTCAAATC+TGG	0.338134	4.4:+86739301	MS.gene068291:CDS
GGTAGTACTATTACTATCTT+GGG	0.346488	4.4:+86739184	MS.gene068291:CDS
TTCATTGAAGGAAAGTATAA+TGG	0.359474	4.4:+86739163	MS.gene068291:CDS
TGGTAGTACTATTACTATCT+TGG	0.363892	4.4:+86739183	MS.gene068291:CDS
TCTGCATCACAGGAAGAAAT+AGG	0.369275	4.4:+86739115	MS.gene068291:CDS
CAATTGAGTTCCAAACTTGT+TGG	0.370040	4.4:+86739070	MS.gene068291:CDS
ATGCCTGTTGTTGGTGGTAG+TGG	0.375988	4.4:+86739238	MS.gene068291:CDS
GGGTGTTGCAAGAGAGTAGA+AGG	0.380408	4.4:-86738793	None:intergenic
GGACTAAACCAAAAGCATTA+AGG	0.382332	4.4:-86739015	None:intergenic
TGAACAATTGTAATTGTTGT+AGG	0.399219	4.4:-86738965	None:intergenic
GAACAAAGGGAGGAAATGTT+TGG	0.416713	4.4:-86738765	None:intergenic
CCTAATAACTTGCGGTCTAT+CGG	0.419137	4.4:-86738875	None:intergenic
GTAGAAGGAAGAAGAACAAA+GGG	0.438676	4.4:-86738778	None:intergenic
ATGCTCAAGCTAGTACTCAT+TGG	0.446414	4.4:+86739284	MS.gene068291:CDS
ATCATGCTCTTGATAAGGTT+AGG	0.460618	4.4:+86739212	MS.gene068291:CDS
CCATTGAAACTACACTCAAC+CGG	0.462163	4.4:+86738992	MS.gene068291:CDS
TGCTCAAGCTAGTACTCATT+GGG	0.464742	4.4:+86739285	MS.gene068291:CDS
CCGGTTGAGTGTAGTTTCAA+TGG	0.466931	4.4:-86738992	None:intergenic
CGTACCAGTAGAATCTGAAA+TGG	0.477796	4.4:-86738922	None:intergenic
TTTCTATGCATCTGCATCAC+AGG	0.485104	4.4:+86739105	MS.gene068291:CDS
AGTAGAAGGAAGAAGAACAA+AGG	0.494125	4.4:-86738779	None:intergenic
GAACAATTGTAATTGTTGTA+GGG	0.501870	4.4:-86738964	None:intergenic
ATTGGGTTGATCTCAAATCT+GGG	0.503082	4.4:+86739302	MS.gene068291:CDS
GTTAGGGAGATGCCTGTTGT+TGG	0.505285	4.4:+86739229	MS.gene068291:CDS
GAAGGAAGAAGAACAAAGGG+AGG	0.511483	4.4:-86738775	None:intergenic
AGTCCACTACCACCAACAAC+AGG	0.512977	4.4:-86739241	None:intergenic
TCATGCTCTTGATAAGGTTA+GGG	0.514769	4.4:+86739213	MS.gene068291:CDS
AAACTTGTTGGAAAAGCTCA+AGG	0.519817	4.4:+86739082	MS.gene068291:CDS
AGGGAGATGCCTGTTGTTGG+TGG	0.521357	4.4:+86739232	MS.gene068291:CDS
GAGGAATCATGCTCTTGATA+AGG	0.521522	4.4:+86739207	MS.gene068291:CDS
AATTGCGGTCCTAAAGTCAA+AGG	0.528813	4.4:-86739052	None:intergenic
TAAGCCATTTCAGATTCTAC+TGG	0.530763	4.4:+86738918	MS.gene068291:CDS
GTTATTAGGCCTAAACAAGA+AGG	0.549483	4.4:+86738889	MS.gene068291:CDS
TGTTTAGGCCTAATAACTTG+CGG	0.555867	4.4:-86738883	None:intergenic
ATTGCGGTCCTAAAGTCAAA+GGG	0.563989	4.4:-86739051	None:intergenic
TACTGGTACGACATACTAAG+TGG	0.577986	4.4:+86738935	MS.gene068291:CDS
TGATGATGAAGTTGATGTGA+GGG	0.600549	4.4:-86738813	None:intergenic
TAAACCAAAAGCATTAAGGC+CGG	0.621311	4.4:-86739011	None:intergenic
ATGATGATGAAGTTGATGTG+AGG	0.637887	4.4:-86738814	None:intergenic
ACAAGTTTGGAACTCAATTG+CGG	0.641411	4.4:-86739067	None:intergenic
GTTGAGTGTAGTTTCAATGG+TGG	0.649150	4.4:-86738989	None:intergenic
TTGGGTTGATCTCAAATCTG+GGG	0.653245	4.4:+86739303	MS.gene068291:CDS
TCAAAGGGCTGTCCATCATG+CGG	0.670156	4.4:-86739036	None:intergenic
GTACTATTACTATCTTGGGG+AGG	0.673978	4.4:+86739188	MS.gene068291:CDS
GTAGTACTATTACTATCTTG+GGG	0.684734	4.4:+86739185	MS.gene068291:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAACAATTGTAATTGTTGTA+GGG	-	chr4.4:86738967-86738986	None:intergenic	25.0%
!	TGAACAATTGTAATTGTTGT+AGG	-	chr4.4:86738968-86738987	None:intergenic	25.0%
!!	ATGAATTTTGCTTTCATTGA+AGG	+	chr4.4:86739151-86739170	MS.gene068291:CDS	25.0%
!!	TTCATTGAAGGAAAGTATAA+TGG	+	chr4.4:86739163-86739182	MS.gene068291:CDS	25.0%
!!!	TTAGTTTTTCCTTCTTGTTT+AGG	-	chr4.4:86738901-86738920	None:intergenic	25.0%
	GGTAGTACTATTACTATCTT+GGG	+	chr4.4:86739184-86739203	MS.gene068291:CDS	30.0%
	GTAGTACTATTACTATCTTG+GGG	+	chr4.4:86739185-86739204	MS.gene068291:CDS	30.0%
	TGGTAGTACTATTACTATCT+TGG	+	chr4.4:86739183-86739202	MS.gene068291:CDS	30.0%
	AGTAGAAGGAAGAAGAACAA+AGG	-	chr4.4:86738782-86738801	None:intergenic	35.0%
	ATCATGCTCTTGATAAGGTT+AGG	+	chr4.4:86739212-86739231	MS.gene068291:CDS	35.0%
	CAATTGAGTTCCAAACTTGT+TGG	+	chr4.4:86739070-86739089	MS.gene068291:CDS	35.0%
	GTAGAAGGAAGAAGAACAAA+GGG	-	chr4.4:86738781-86738800	None:intergenic	35.0%
	GTTATTAGGCCTAAACAAGA+AGG	+	chr4.4:86738889-86738908	MS.gene068291:CDS	35.0%
	TAAGCCATTTCAGATTCTAC+TGG	+	chr4.4:86738918-86738937	MS.gene068291:CDS	35.0%
	TCATGCTCTTGATAAGGTTA+GGG	+	chr4.4:86739213-86739232	MS.gene068291:CDS	35.0%
	TGTTTAGGCCTAATAACTTG+CGG	-	chr4.4:86738886-86738905	None:intergenic	35.0%
!	ACAAGTTTGGAACTCAATTG+CGG	-	chr4.4:86739070-86739089	None:intergenic	35.0%
!	TTGAGCTTTTCCAACAAGTT+TGG	-	chr4.4:86739083-86739102	None:intergenic	35.0%
!!	AAACTTGTTGGAAAAGCTCA+AGG	+	chr4.4:86739082-86739101	MS.gene068291:CDS	35.0%
!!	ATGATGATGAAGTTGATGTG+AGG	-	chr4.4:86738817-86738836	None:intergenic	35.0%
!!	ATTGGGTTGATCTCAAATCT+GGG	+	chr4.4:86739302-86739321	MS.gene068291:CDS	35.0%
!!	GGACTAAACCAAAAGCATTA+AGG	-	chr4.4:86739018-86739037	None:intergenic	35.0%
!!	TAAACCAAAAGCATTAAGGC+CGG	-	chr4.4:86739014-86739033	None:intergenic	35.0%
!!	TGATGATGAAGTTGATGTGA+GGG	-	chr4.4:86738816-86738835	None:intergenic	35.0%
!!!	GACTTTTCAGATTTGCTAGA+GGG	+	chr4.4:86739260-86739279	MS.gene068291:CDS	35.0%
	AATTGCGGTCCTAAAGTCAA+AGG	-	chr4.4:86739055-86739074	None:intergenic	40.0%
	ATGCTCAAGCTAGTACTCAT+TGG	+	chr4.4:86739284-86739303	MS.gene068291:CDS	40.0%
	ATTGCGGTCCTAAAGTCAAA+GGG	-	chr4.4:86739054-86739073	None:intergenic	40.0%
	CCATTGAAACTACACTCAAC+CGG	+	chr4.4:86738992-86739011	MS.gene068291:CDS	40.0%
	CGTACCAGTAGAATCTGAAA+TGG	-	chr4.4:86738925-86738944	None:intergenic	40.0%
	GAACAAAGGGAGGAAATGTT+TGG	-	chr4.4:86738768-86738787	None:intergenic	40.0%
	GAGGAATCATGCTCTTGATA+AGG	+	chr4.4:86739207-86739226	MS.gene068291:CDS	40.0%
	GTACTATTACTATCTTGGGG+AGG	+	chr4.4:86739188-86739207	MS.gene068291:CDS	40.0%
	GTTGAGTGTAGTTTCAATGG+TGG	-	chr4.4:86738992-86739011	None:intergenic	40.0%
	TACTGGTACGACATACTAAG+TGG	+	chr4.4:86738935-86738954	MS.gene068291:CDS	40.0%
	TCTGCATCACAGGAAGAAAT+AGG	+	chr4.4:86739115-86739134	MS.gene068291:CDS	40.0%
	TGCTCAAGCTAGTACTCATT+GGG	+	chr4.4:86739285-86739304	MS.gene068291:CDS	40.0%
	TTTCTATGCATCTGCATCAC+AGG	+	chr4.4:86739105-86739124	MS.gene068291:CDS	40.0%
!	CCTAATAACTTGCGGTCTAT+CGG	-	chr4.4:86738878-86738897	None:intergenic	40.0%
!!	CATTGGGTTGATCTCAAATC+TGG	+	chr4.4:86739301-86739320	MS.gene068291:CDS	40.0%
!!	TTGGGTTGATCTCAAATCTG+GGG	+	chr4.4:86739303-86739322	MS.gene068291:CDS	40.0%
!!!	GGACTTTTCAGATTTGCTAG+AGG	+	chr4.4:86739259-86739278	MS.gene068291:CDS	40.0%
!!!	TTTTGGTTTAGTCCGCATGA+TGG	+	chr4.4:86739024-86739043	MS.gene068291:CDS	40.0%
	CCGATAGACCGCAAGTTATT+AGG	+	chr4.4:86738875-86738894	MS.gene068291:CDS	45.0%
	CCGGTTGAGTGTAGTTTCAA+TGG	-	chr4.4:86738995-86739014	None:intergenic	45.0%
	GAAGGAAGAAGAACAAAGGG+AGG	-	chr4.4:86738778-86738797	None:intergenic	45.0%
!	ATGGACAGCCCTTTGACTTT+AGG	+	chr4.4:86739043-86739062	MS.gene068291:CDS	45.0%
!	TCAACCGGCCTTAATGCTTT+TGG	+	chr4.4:86739007-86739026	MS.gene068291:CDS	45.0%
	AGTCCACTACCACCAACAAC+AGG	-	chr4.4:86739244-86739263	None:intergenic	50.0%
	GTTAGGGAGATGCCTGTTGT+TGG	+	chr4.4:86739229-86739248	MS.gene068291:CDS	50.0%
	TCAAAGGGCTGTCCATCATG+CGG	-	chr4.4:86739039-86739058	None:intergenic	50.0%
!	GGGTGTTGCAAGAGAGTAGA+AGG	-	chr4.4:86738796-86738815	None:intergenic	50.0%
!!	ATGCCTGTTGTTGGTGGTAG+TGG	+	chr4.4:86739238-86739257	MS.gene068291:CDS	50.0%
	AGGGAGATGCCTGTTGTTGG+TGG	+	chr4.4:86739232-86739251	MS.gene068291:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	86738758	86739366	86738758	ID=MS.gene068291
chr4.4	mRNA	86738758	86739366	86738758	ID=MS.gene068291.t1;Parent=MS.gene068291
chr4.4	exon	86738758	86739366	86738758	ID=MS.gene068291.t1.exon1;Parent=MS.gene068291.t1
chr4.4	CDS	86738758	86739366	86738758	ID=cds.MS.gene068291.t1;Parent=MS.gene068291.t1

Gene Sequence

>MS.gene068291

ATGTCTTCCAAACATTTCCTCCCTTTGTTCTTCTTCCTTCTACTCTCTTGCAACACCCTCACATCAACTTCATCATCATCATCATCAGAAAATGAAGAAACTTTTGATTTCGTTCGTCCGATAGACCGCAAGTTATTAGGCCTAAACAAGAAGGAAAAACTAAGCCATTTCAGATTCTACTGGTACGACATACTAAGTGGAAATAACCCTACAACAATTACAATTGTTCAGCCACCATTGAAACTACACTCAACCGGCCTTAATGCTTTTGGTTTAGTCCGCATGATGGACAGCCCTTTGACTTTAGGACCGCAATTGAGTTCCAAACTTGTTGGAAAAGCTCAAGGTTTCTATGCATCTGCATCACAGGAAGAAATAGGTTTTCTTATGACAATGAATTTTGCTTTCATTGAAGGAAAGTATAATGGTAGTACTATTACTATCTTGGGGAGGAATCATGCTCTTGATAAGGTTAGGGAGATGCCTGTTGTTGGTGGTAGTGGACTTTTCAGATTTGCTAGAGGGTATGCTCAAGCTAGTACTCATTGGGTTGATCTCAAATCTGGGGATGCATGTGTTGAGTACAATCTTCATGTTTTTCACTATTGA

Protein sequence

>MS.gene068291.t1

MSSKHFLPLFFFLLLSCNTLTSTSSSSSSENEETFDFVRPIDRKLLGLNKKEKLSHFRFYWYDILSGNNPTTITIVQPPLKLHSTGLNAFGLVRMMDSPLTLGPQLSSKLVGKAQGFYASASQEEIGFLMTMNFAFIEGKYNGSTITILGRNHALDKVREMPVVGGSGLFRFARGYAQASTHWVDLKSGDACVEYNLHVFHY