AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33424

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33424.t1 MTR_4g013330 88.442 199 23 0 17 215 1 199 2.07e-130 365
MS.gene33424.t1 MTR_4g013350 81.910 199 34 1 17 215 1 197 2.20e-119 337
MS.gene33424.t1 MTR_4g013345 79.899 199 37 2 17 215 1 196 4.81e-112 318
MS.gene33424.t1 MTR_4g013320 76.882 186 39 2 32 215 16 199 2.56e-105 301
MS.gene33424.t1 MTR_4g013335 75.500 200 44 2 17 215 1 196 1.64e-104 300
MS.gene33424.t1 MTR_4g013310 65.672 201 63 3 17 215 1 197 7.91e-89 260
MS.gene33424.t1 MTR_4g013325 70.297 202 54 3 17 215 1 199 1.04e-88 259
MS.gene33424.t1 MTR_7g070390 64.583 192 62 3 25 215 6 192 1.01e-78 234
MS.gene33424.t1 MTR_4g013385 56.842 190 74 3 26 215 9 190 3.24e-73 220
MS.gene33424.t1 MTR_0433s0040 56.842 190 74 3 26 215 9 190 3.24e-73 220
MS.gene33424.t1 MTR_4g013770 58.480 171 68 2 45 215 24 191 1.13e-66 204
MS.gene33424.t1 MTR_1g054525 53.535 198 79 4 19 215 7 192 5.33e-65 199
MS.gene33424.t1 MTR_4g013355 50.000 188 77 4 28 215 9 179 2.92e-58 182
MS.gene33424.t1 MTR_4g073950 54.248 153 69 1 63 215 41 192 7.42e-58 181
MS.gene33424.t1 MTR_7g093870 53.205 156 70 1 63 215 38 193 1.63e-54 174
MS.gene33424.t1 MTR_7g093850 49.468 188 90 4 29 215 11 194 3.72e-54 172
MS.gene33424.t1 MTR_7g093820 49.474 190 87 5 29 215 11 194 1.01e-53 171
MS.gene33424.t1 MTR_7g093790 42.647 204 100 4 13 215 4 191 3.67e-53 169
MS.gene33424.t1 MTR_7g093830 45.361 194 94 4 23 215 11 193 7.42e-53 168
MS.gene33424.t1 MTR_1g056370 49.673 153 73 2 63 214 36 185 8.08e-49 158
MS.gene33424.t1 MTR_4g078885 46.358 151 77 1 65 215 39 185 4.52e-45 148
MS.gene33424.t1 MTR_3g034030 46.358 151 77 1 65 215 39 185 1.24e-44 147
MS.gene33424.t1 MTR_3g105640 43.827 162 89 2 55 215 28 188 4.42e-44 146
MS.gene33424.t1 MTR_3g105630 43.396 159 89 1 58 215 31 189 7.37e-43 143
MS.gene33424.t1 MTR_5g096120 41.026 156 82 3 66 215 38 189 6.02e-38 130
MS.gene33424.t1 MTR_4g122110 35.811 148 85 2 72 215 39 180 1.34e-23 93.2
MS.gene33424.t1 MTR_4g122130 34.932 146 89 2 72 215 39 180 5.34e-23 91.7
MS.gene33424.t1 MTR_8g099115 35.294 136 81 4 65 194 34 168 4.03e-15 70.9
MS.gene33424.t1 MTR_4g049550 31.298 131 84 2 62 190 27 153 4.45e-15 70.9
MS.gene33424.t1 MTR_4g049570 28.767 146 102 1 65 210 30 173 8.26e-14 67.4
MS.gene33424.t1 MTR_8g099135 32.044 181 90 7 24 194 7 164 7.97e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33424.t1 AT1G55210 58.421 190 72 3 26 215 5 187 4.19e-78 233
MS.gene33424.t1 AT1G55210 58.421 190 72 3 26 215 5 187 4.19e-78 233
MS.gene33424.t1 AT1G58170 57.292 192 69 3 26 215 5 185 2.49e-74 223
MS.gene33424.t1 AT5G49040 53.684 190 86 1 26 215 4 191 3.85e-70 212
MS.gene33424.t1 AT3G13650 53.889 180 75 4 36 215 15 186 5.79e-65 199
MS.gene33424.t1 AT3G13662 56.291 151 61 1 52 202 27 172 7.24e-56 176
MS.gene33424.t1 AT5G49030 51.323 189 82 3 22 210 6 184 3.00e-55 189
MS.gene33424.t1 AT1G65870 48.366 153 77 1 62 214 39 189 3.71e-52 167
MS.gene33424.t1 AT3G13660 57.812 128 51 1 88 215 1 125 4.59e-49 156
MS.gene33424.t1 AT2G21100 48.344 151 76 1 64 214 39 187 9.73e-49 158
MS.gene33424.t1 AT2G21100 48.344 151 76 1 64 214 39 187 9.73e-49 158
MS.gene33424.t1 AT1G22900 49.673 153 73 2 62 214 45 193 9.80e-49 158
MS.gene33424.t1 AT5G42500 48.026 152 75 3 63 214 38 185 3.59e-46 151
MS.gene33424.t1 AT5G42510 46.405 153 76 3 63 214 35 182 1.00e-44 147
MS.gene33424.t1 AT4G38700 37.949 195 109 5 25 215 4 190 2.33e-39 134
MS.gene33424.t1 AT2G21110 39.306 173 91 3 48 215 23 186 2.57e-37 129
MS.gene33424.t1 AT3G58090 50.000 110 55 0 105 214 162 271 1.35e-35 127
MS.gene33424.t1 AT4G23690 35.507 138 80 4 61 194 34 166 1.59e-16 74.7
MS.gene33424.t1 AT4G11210 28.649 185 110 6 17 198 1 166 2.20e-14 68.9
MS.gene33424.t1 AT4G11190 28.495 186 109 6 17 198 1 166 1.17e-13 67.0
MS.gene33424.t1 AT4G11180 27.604 192 119 5 26 214 9 183 1.60e-12 63.9

Find 48 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATAGACCGCAAGTTATT+AGG 0.263716 4.2:+87145025 MS.gene33424:CDS
ATGGACAGCCCTTTGACTTT+AGG 0.267915 4.2:+87145193 MS.gene33424:CDS
TTTATTGAAGGAAAGTATAA+TGG 0.272706 4.2:+87145313 MS.gene33424:CDS
CCTAATAACTTGCGGTCTAT+TGG 0.300244 4.2:-87145025 None:intergenic
TCAACCGGCCTTAATGCTTT+TGG 0.320368 4.2:+87145157 MS.gene33424:CDS
CAATTGAGTTCCAAGCTTGT+AGG 0.324819 4.2:+87145220 MS.gene33424:CDS
CATTGGGTTGATCTCAAATC+TGG 0.338134 4.2:+87145451 MS.gene33424:CDS
GAAATGTTTGGAAGGCATTA+TGG 0.353762 4.2:-87144912 None:intergenic
TGGGAGTACTCTTACTATCT+TGG 0.368563 4.2:+87145333 MS.gene33424:CDS
TCTGCATCACAGGAAGAAAT+AGG 0.369275 4.2:+87145265 MS.gene33424:CDS
CAAAGTGATGAGGAAATGTT+TGG 0.371335 4.2:-87144924 None:intergenic
ATGCCTGTTGTTGGTGGTAG+TGG 0.375988 4.2:+87145388 MS.gene33424:CDS
GGGAGTACTCTTACTATCTT+GGG 0.379918 4.2:+87145334 MS.gene33424:CDS
GGACTAAACCAAAAGCATTA+AGG 0.382332 4.2:-87145165 None:intergenic
TGAACAATTGTAATTGTTGT+AGG 0.389348 4.2:-87145115 None:intergenic
TTATTGAAGGAAAGTATAAT+GGG 0.420383 4.2:+87145314 MS.gene33424:CDS
ATGCTCAAGCTAGTACTCAT+TGG 0.446414 4.2:+87145434 MS.gene33424:CDS
ATCCTGCTATTGATAAGGTT+AGG 0.457162 4.2:+87145362 MS.gene33424:CDS
TGCTCAAGCTAGTACTCATT+GGG 0.464742 4.2:+87145435 MS.gene33424:CDS
CCGGTTGAGTGTAGTTTCAA+TGG 0.466931 4.2:-87145142 None:intergenic
CCATTGAAACTACACTCAAC+CGG 0.471757 4.2:+87145142 MS.gene33424:CDS
TTTCTATGCATCTGCATCAC+AGG 0.485104 4.2:+87145255 MS.gene33424:CDS
GAACAATTGTAATTGTTGTA+GGG 0.491999 4.2:-87145114 None:intergenic
TCCCTAACCTTATCAATAGC+AGG 0.497267 4.2:-87145364 None:intergenic
TCCTGCTATTGATAAGGTTA+GGG 0.497387 4.2:+87145363 MS.gene33424:CDS
GTGTGTTGCAAGATATGAGA+AGG 0.498448 4.2:-87144952 None:intergenic
ATTGGGTTGATCTCAAATCT+GGG 0.503082 4.2:+87145452 MS.gene33424:CDS
CGTGCCAGTAGAATCTGAAA+TGG 0.504595 4.2:-87145072 None:intergenic
GTTAGGGAGATGCCTGTTGT+TGG 0.505285 4.2:+87145379 MS.gene33424:CDS
GAGGAATCCTGCTATTGATA+AGG 0.510518 4.2:+87145357 MS.gene33424:CDS
AGTCCACTACCACCAACAAC+AGG 0.512977 4.2:-87145391 None:intergenic
AATTGCAGTCCTAAAGTCAA+AGG 0.516117 4.2:-87145202 None:intergenic
AGGGAGATGCCTGTTGTTGG+TGG 0.521357 4.2:+87145382 MS.gene33424:CDS
TAAGCCATTTCAGATTCTAC+TGG 0.525620 4.2:+87145068 MS.gene33424:CDS
GTTATTAGGCCTAAACAAGA+AGG 0.549483 4.2:+87145039 MS.gene33424:CDS
TGTTTAGGCCTAATAACTTG+CGG 0.555867 4.2:-87145033 None:intergenic
ATTGCAGTCCTAAAGTCAAA+GGG 0.561693 4.2:-87145201 None:intergenic
GTGATGAGGAAATGTTTGGA+AGG 0.586936 4.2:-87144920 None:intergenic
TACTGGCACGACATACTAAG+TGG 0.588416 4.2:+87145085 MS.gene33424:CDS
GAAGGAAGAACAAAGTGATG+AGG 0.589675 4.2:-87144934 None:intergenic
AAGCTTGTAGGAAAAGCTCA+AGG 0.610963 4.2:+87145232 MS.gene33424:CDS
TAAACCAAAAGCATTAAGGC+CGG 0.621311 4.2:-87145161 None:intergenic
GTTGAGTGTAGTTTCAATGG+TGG 0.624193 4.2:-87145139 None:intergenic
ATGTTTGGAAGGCATTATGG+TGG 0.626853 4.2:-87144909 None:intergenic
TTGGGTTGATCTCAAATCTG+GGG 0.653245 4.2:+87145453 MS.gene33424:CDS
TCAAAGGGCTGTCCATCATG+CGG 0.670156 4.2:-87145186 None:intergenic
GGAGTACTCTTACTATCTTG+GGG 0.671558 4.2:+87145335 MS.gene33424:CDS
GTACTCTTACTATCTTGGGG+AGG 0.673978 4.2:+87145338 MS.gene33424:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTTAAACATTTTGTTT+TGG + chr4.2:87144509-87144528 MS.gene33424:intron 10.0%
!! ATAAAATAAAACTATATGAC+AGG - chr4.2:87144491-87144510 None:intergenic 15.0%
!!! ATATTTTAAGCTTTTATATG+TGG + chr4.2:87144549-87144568 MS.gene33424:intron 15.0%
!!! TTTTTTTAACAAGCAAAAAT+CGG - chr4.2:87144717-87144736 None:intergenic 15.0%
!!! ATGAATTTTGCTTTTATTGA+AGG + chr4.2:87145301-87145320 MS.gene33424:CDS 20.0%
!!! CTAATCTTTTTGTTTTTCTT+TGG + chr4.2:87144604-87144623 MS.gene33424:intron 20.0%
!!! TTATTGAAGGAAAGTATAAT+GGG + chr4.2:87145314-87145333 MS.gene33424:CDS 20.0%
!!! TTTATTGAAGGAAAGTATAA+TGG + chr4.2:87145313-87145332 MS.gene33424:CDS 20.0%
! AGGAACAAAAATATAAGAAG+AGG - chr4.2:87144809-87144828 None:intergenic 25.0%
! ATAAGAAGTTAAAGAAAGTG+AGG - chr4.2:87144829-87144848 None:intergenic 25.0%
! GAACAATTGTAATTGTTGTA+GGG - chr4.2:87145117-87145136 None:intergenic 25.0%
! TGAACAATTGTAATTGTTGT+AGG - chr4.2:87145118-87145137 None:intergenic 25.0%
!! TTTTAAGCTTTTATATGTGG+TGG + chr4.2:87144552-87144571 MS.gene33424:intron 25.0%
!!! TTAGTTTTTCCTTCTTGTTT+AGG - chr4.2:87145051-87145070 None:intergenic 25.0%
ATATAAGAAGAGGAAAGTGA+AGG - chr4.2:87144799-87144818 None:intergenic 30.0%
! AGTGAAGGAAAAAGTACAAA+TGG - chr4.2:87144784-87144803 None:intergenic 30.0%
AATTGCAGTCCTAAAGTCAA+AGG - chr4.2:87145205-87145224 None:intergenic 35.0%
ATCCTGCTATTGATAAGGTT+AGG + chr4.2:87145362-87145381 MS.gene33424:CDS 35.0%
ATTGCAGTCCTAAAGTCAAA+GGG - chr4.2:87145204-87145223 None:intergenic 35.0%
CAAAGTGATGAGGAAATGTT+TGG - chr4.2:87144927-87144946 None:intergenic 35.0%
GTTATTAGGCCTAAACAAGA+AGG + chr4.2:87145039-87145058 MS.gene33424:CDS 35.0%
TAAGCCATTTCAGATTCTAC+TGG + chr4.2:87145068-87145087 MS.gene33424:CDS 35.0%
TCCTGCTATTGATAAGGTTA+GGG + chr4.2:87145363-87145382 MS.gene33424:CDS 35.0%
TGTTTAGGCCTAATAACTTG+CGG - chr4.2:87145036-87145055 None:intergenic 35.0%
! AGGAAAAAGTACAAATGGAG+TGG - chr4.2:87144779-87144798 None:intergenic 35.0%
! GGAAAAAGTACAAATGGAGT+GGG - chr4.2:87144778-87144797 None:intergenic 35.0%
!! ATTGGGTTGATCTCAAATCT+GGG + chr4.2:87145452-87145471 MS.gene33424:CDS 35.0%
!! GAAATGTTTGGAAGGCATTA+TGG - chr4.2:87144915-87144934 None:intergenic 35.0%
!! GGACTAAACCAAAAGCATTA+AGG - chr4.2:87145168-87145187 None:intergenic 35.0%
!! TAAACCAAAAGCATTAAGGC+CGG - chr4.2:87145164-87145183 None:intergenic 35.0%
!!! GACTTTTCAGATTTGCTAGA+GGG + chr4.2:87145410-87145429 MS.gene33424:CDS 35.0%
AAGCTTGTAGGAAAAGCTCA+AGG + chr4.2:87145232-87145251 MS.gene33424:CDS 40.0%
ACAAATGGAGTGGGATGTAT+AGG - chr4.2:87144769-87144788 None:intergenic 40.0%
ATGCTCAAGCTAGTACTCAT+TGG + chr4.2:87145434-87145453 MS.gene33424:CDS 40.0%
CAAATGGAGTGGGATGTATA+GGG - chr4.2:87144768-87144787 None:intergenic 40.0%
CAATGCTCAGACAAACAAAG+AGG - chr4.2:87144583-87144602 None:intergenic 40.0%
CAATTGAGTTCCAAGCTTGT+AGG + chr4.2:87145220-87145239 MS.gene33424:CDS 40.0%
CCAATAGACCGCAAGTTATT+AGG + chr4.2:87145025-87145044 MS.gene33424:CDS 40.0%
CCATTGAAACTACACTCAAC+CGG + chr4.2:87145142-87145161 MS.gene33424:CDS 40.0%
CTGTGGAGACAATAAAGTAG+TGG - chr4.2:87144864-87144883 None:intergenic 40.0%
GAAGGAAGAACAAAGTGATG+AGG - chr4.2:87144937-87144956 None:intergenic 40.0%
GAGGAATCCTGCTATTGATA+AGG + chr4.2:87145357-87145376 MS.gene33424:CDS 40.0%
GATTGTTGTGATGCTAACTG+TGG - chr4.2:87144881-87144900 None:intergenic 40.0%
GTGATGAGGAAATGTTTGGA+AGG - chr4.2:87144923-87144942 None:intergenic 40.0%
GTGTGTTGCAAGATATGAGA+AGG - chr4.2:87144955-87144974 None:intergenic 40.0%
GTTGAGTGTAGTTTCAATGG+TGG - chr4.2:87145142-87145161 None:intergenic 40.0%
TCCCTAACCTTATCAATAGC+AGG - chr4.2:87145367-87145386 None:intergenic 40.0%
TCTGCATCACAGGAAGAAAT+AGG + chr4.2:87145265-87145284 MS.gene33424:CDS 40.0%
TGCTCAAGCTAGTACTCATT+GGG + chr4.2:87145435-87145454 MS.gene33424:CDS 40.0%
TGGAGACAATAAAGTAGTGG+AGG - chr4.2:87144861-87144880 None:intergenic 40.0%
TTTCTATGCATCTGCATCAC+AGG + chr4.2:87145255-87145274 MS.gene33424:CDS 40.0%
! CCTAATAACTTGCGGTCTAT+TGG - chr4.2:87145028-87145047 None:intergenic 40.0%
! GGAGTACTCTTACTATCTTG+GGG + chr4.2:87145335-87145354 MS.gene33424:CDS 40.0%
! TTGAGCTTTTCCTACAAGCT+TGG - chr4.2:87145233-87145252 None:intergenic 40.0%
!! ATGTTTGGAAGGCATTATGG+TGG - chr4.2:87144912-87144931 None:intergenic 40.0%
!! CATTGGGTTGATCTCAAATC+TGG + chr4.2:87145451-87145470 MS.gene33424:CDS 40.0%
!! GAAGAGCACTTAAAAGCACA+AGG - chr4.2:87144675-87144694 None:intergenic 40.0%
!! GGGAGTACTCTTACTATCTT+GGG + chr4.2:87145334-87145353 MS.gene33424:CDS 40.0%
!! TGCTTTTAAGTGCTCTTCGT+TGG + chr4.2:87144677-87144696 MS.gene33424:intron 40.0%
!! TGGGAGTACTCTTACTATCT+TGG + chr4.2:87145333-87145352 MS.gene33424:CDS 40.0%
!! TTGGGTTGATCTCAAATCTG+GGG + chr4.2:87145453-87145472 MS.gene33424:CDS 40.0%
!!! GGACTTTTCAGATTTGCTAG+AGG + chr4.2:87145409-87145428 MS.gene33424:CDS 40.0%
!!! TTTTGGTTTAGTCCGCATGA+TGG + chr4.2:87145174-87145193 MS.gene33424:CDS 40.0%
CCGGTTGAGTGTAGTTTCAA+TGG - chr4.2:87145145-87145164 None:intergenic 45.0%
CGTGCCAGTAGAATCTGAAA+TGG - chr4.2:87145075-87145094 None:intergenic 45.0%
GTACTCTTACTATCTTGGGG+AGG + chr4.2:87145338-87145357 MS.gene33424:CDS 45.0%
TACTGGCACGACATACTAAG+TGG + chr4.2:87145085-87145104 MS.gene33424:CDS 45.0%
! ATGGACAGCCCTTTGACTTT+AGG + chr4.2:87145193-87145212 MS.gene33424:CDS 45.0%
! TCAACCGGCCTTAATGCTTT+TGG + chr4.2:87145157-87145176 MS.gene33424:CDS 45.0%
AGTCCACTACCACCAACAAC+AGG - chr4.2:87145394-87145413 None:intergenic 50.0%
GTTAGGGAGATGCCTGTTGT+TGG + chr4.2:87145379-87145398 MS.gene33424:CDS 50.0%
TCAAAGGGCTGTCCATCATG+CGG - chr4.2:87145189-87145208 None:intergenic 50.0%
!! ATGCCTGTTGTTGGTGGTAG+TGG + chr4.2:87145388-87145407 MS.gene33424:CDS 50.0%
AGGGAGATGCCTGTTGTTGG+TGG + chr4.2:87145382-87145401 MS.gene33424:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 87144467 87145516 87144467 ID=MS.gene33424
chr4.2 mRNA 87144467 87145516 87144467 ID=MS.gene33424.t1;Parent=MS.gene33424
chr4.2 exon 87144467 87144481 87144467 ID=MS.gene33424.t1.exon1;Parent=MS.gene33424.t1
chr4.2 CDS 87144467 87144481 87144467 ID=cds.MS.gene33424.t1;Parent=MS.gene33424.t1
chr4.2 exon 87144884 87145516 87144884 ID=MS.gene33424.t1.exon2;Parent=MS.gene33424.t1
chr4.2 CDS 87144884 87145516 87144884 ID=cds.MS.gene33424.t1;Parent=MS.gene33424.t1
Gene Sequence

>MS.gene33424

ATGATCTCGTTCATTGTAAAACCTGTCATATAGTTTTATTTTATTATTTAAACATTTTGTTTTGGTAGAAATATTTTTAGACATATTTTAAGCTTTTATATGTGGTGGTCGAGCCTCTTTGTTTGTCTGAGCATTGTCTAATCTTTTTGTTTTTCTTTGGTTTCAACTGATTTGTTTGTAAACTCTTGAAGTCTTTGTTGACACACCTTGTGCTTTTAAGTGCTCTTCGTTGGTGTTTATATATAATCCGATTTTTGCTTGTTAAAAAAAAAGAATGAAATAATTAGAAAAATACTTACCCTATACATCCCACTCCATTTGTACTTTTTCCTTCACTTTCCTCTTCTTATATTTTTGTTCCTCACTTTCTTTAACTTCTTATATTTTCTCTCCTCCACTACTTTATTGTCTCCACAGTTAGCATCACAACAATCTCTTGCATCCACCATAATGCCTTCCAAACATTTCCTCATCACTTTGTTCTTCCTTCTCATATCTTGCAACACACTCTCTGCTTCAACTTCATCAGAAAAAGAAGACACTTTTGATTTTGTTCGTCCAATAGACCGCAAGTTATTAGGCCTAAACAAGAAGGAAAAACTAAGCCATTTCAGATTCTACTGGCACGACATACTAAGTGGAGATAACCCTACAACAATTACAATTGTTCAACCACCATTGAAACTACACTCAACCGGCCTTAATGCTTTTGGTTTAGTCCGCATGATGGACAGCCCTTTGACTTTAGGACTGCAATTGAGTTCCAAGCTTGTAGGAAAAGCTCAAGGTTTCTATGCATCTGCATCACAGGAAGAAATAGGTTTTCTTATGACAATGAATTTTGCTTTTATTGAAGGAAAGTATAATGGGAGTACTCTTACTATCTTGGGGAGGAATCCTGCTATTGATAAGGTTAGGGAGATGCCTGTTGTTGGTGGTAGTGGACTTTTCAGATTTGCTAGAGGGTATGCTCAAGCTAGTACTCATTGGGTTGATCTCAAATCTGGGGATGCATGTGTTGAGTACAATCTTCATGTTTTTCACTACTGA
Protein sequence

>MS.gene33424.t1

MISFILASQQSLASTIMPSKHFLITLFFLLISCNTLSASTSSEKEDTFDFVRPIDRKLLGLNKKEKLSHFRFYWHDILSGDNPTTITIVQPPLKLHSTGLNAFGLVRMMDSPLTLGLQLSSKLVGKAQGFYASASQEEIGFLMTMNFAFIEGKYNGSTLTILGRNPAIDKVREMPVVGGSGLFRFARGYAQASTHWVDLKSGDACVEYNLHVFHY