AlfalfaGEDB | MS.gene33426

M. sativa cultivar XinJiangDaYe / MS.gene33426

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33426.t1	XP_013454799.1	96.5	198	6	1	1	198	1	197	1.20E-105	392.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33426.t1	Q9C891	59.8	189	71	2	10	198	4	187	1.4e-59	230.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33426.t1	A0A072USJ4	96.5	198	6	1	1	198	1	197	8.3e-106	392.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050780	MS.gene33426	0.804862	1.76E-49	-1.69E-46
MS.gene051492	MS.gene33426	0.808298	3.32E-50	-1.69E-46
MS.gene053094	MS.gene33426	0.832748	7.92E-56	-1.69E-46
MS.gene053643	MS.gene33426	0.803147	4.01E-49	-1.69E-46
MS.gene053963	MS.gene33426	0.801938	7.11E-49	-1.69E-46
MS.gene05410	MS.gene33426	0.827056	1.93E-54	-1.69E-46
MS.gene05414	MS.gene33426	0.808476	3.04E-50	-1.69E-46
MS.gene054576	MS.gene33426	0.824963	6.07E-54	-1.69E-46
MS.gene060520	MS.gene33426	0.800423	1.45E-48	-1.69E-46
MS.gene060840	MS.gene33426	0.807282	5.46E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene33679	MS.gene33426	PPI
MS.gene33420	MS.gene33426	PPI
MS.gene33426	MS.gene068292	PPI
MS.gene003512	MS.gene33426	PPI
MS.gene24971	MS.gene33426	PPI
MS.gene33426	MS.gene06033	PPI
MS.gene33426	MS.gene90969	PPI
MS.gene24973	MS.gene33426	PPI
MS.gene33426	MS.gene33676	PPI
MS.gene068291	MS.gene33426	PPI
MS.gene33418	MS.gene33426	PPI
MS.gene048891	MS.gene33426	PPI
MS.gene55764	MS.gene33426	PPI
MS.gene071329	MS.gene33426	PPI
MS.gene33681	MS.gene33426	PPI
MS.gene98692	MS.gene33426	PPI
MS.gene33424	MS.gene33426	PPI
MS.gene06035	MS.gene33426	PPI
MS.gene057717	MS.gene33426	PPI
MS.gene24969	MS.gene33426	PPI
MS.gene33677	MS.gene33426	PPI
MS.gene33680	MS.gene33426	PPI
MS.gene55766	MS.gene33426	PPI
MS.gene024359	MS.gene33426	PPI
MS.gene52084	MS.gene33426	PPI
MS.gene068294	MS.gene33426	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33426.t1	MTR_4g013350	94.949	198	9	1	1	198	1	197	2.35e-136	379
MS.gene33426.t1	MTR_4g013345	90.710	183	16	1	16	198	15	196	3.88e-123	346
MS.gene33426.t1	MTR_4g013330	84.239	184	27	1	17	198	16	199	2.55e-118	334
MS.gene33426.t1	MTR_4g013335	79.798	198	38	1	1	198	1	196	4.34e-113	320
MS.gene33426.t1	MTR_4g013320	78.804	184	37	1	17	198	16	199	2.52e-110	313
MS.gene33426.t1	MTR_4g013325	76.119	201	43	3	1	198	1	199	3.54e-93	270
MS.gene33426.t1	MTR_4g013310	67.500	200	60	3	1	198	1	197	3.94e-93	270
MS.gene33426.t1	MTR_7g070390	67.010	194	60	3	6	198	2	192	1.20e-83	246
MS.gene33426.t1	MTR_4g013385	60.317	189	69	3	10	198	8	190	1.15e-77	231
MS.gene33426.t1	MTR_0433s0040	60.317	189	69	3	10	198	8	190	1.15e-77	231
MS.gene33426.t1	MTR_4g013770	62.353	170	61	2	30	198	24	191	2.11e-70	212
MS.gene33426.t1	MTR_1g054525	56.633	196	75	3	3	198	7	192	4.64e-70	211
MS.gene33426.t1	MTR_4g013355	54.497	189	71	3	10	198	6	179	2.79e-65	199
MS.gene33426.t1	MTR_4g073950	56.579	152	65	1	48	198	41	192	1.98e-61	189
MS.gene33426.t1	MTR_7g093850	50.000	186	90	3	14	198	11	194	3.14e-59	184
MS.gene33426.t1	MTR_7g093820	50.000	186	90	3	14	198	11	194	4.71e-59	184
MS.gene33426.t1	MTR_7g093830	47.849	186	94	3	14	198	10	193	9.97e-58	180
MS.gene33426.t1	MTR_7g093790	48.795	166	83	2	34	198	27	191	1.17e-57	180
MS.gene33426.t1	MTR_7g093870	53.205	156	68	2	48	198	38	193	9.25e-55	173
MS.gene33426.t1	MTR_1g056370	49.669	151	74	2	48	197	36	185	2.30e-48	156
MS.gene33426.t1	MTR_3g105640	47.826	161	82	2	40	198	28	188	7.74e-48	155
MS.gene33426.t1	MTR_3g105630	41.969	193	101	3	9	198	5	189	2.44e-46	151
MS.gene33426.t1	MTR_4g078885	47.651	149	76	1	50	198	39	185	3.85e-45	148
MS.gene33426.t1	MTR_3g034030	48.322	149	75	1	50	198	39	185	7.07e-45	147
MS.gene33426.t1	MTR_5g096120	44.231	156	75	3	51	198	38	189	3.45e-41	138
MS.gene33426.t1	MTR_4g122110	37.324	142	89	0	57	198	39	180	1.24e-28	105
MS.gene33426.t1	MTR_4g122130	36.620	142	90	0	57	198	39	180	5.68e-26	99.0
MS.gene33426.t1	MTR_4g049550	31.293	147	101	0	47	193	27	173	2.05e-18	79.0
MS.gene33426.t1	MTR_4g049570	30.769	143	99	0	51	193	31	173	1.86e-17	76.6
MS.gene33426.t1	MTR_8g099115	33.824	136	81	3	50	177	34	168	2.55e-16	73.9
MS.gene33426.t1	MTR_8g099135	34.091	132	70	4	58	177	38	164	5.62e-14	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33426.t1	AT1G55210	59.788	189	71	2	10	198	4	187	4.85e-80	236
MS.gene33426.t1	AT1G55210	59.788	189	71	2	10	198	4	187	4.85e-80	236
MS.gene33426.t1	AT1G58170	57.979	188	68	2	13	198	7	185	6.80e-76	226
MS.gene33426.t1	AT5G49040	54.450	191	87	0	8	198	1	191	2.17e-75	225
MS.gene33426.t1	AT3G13650	52.356	191	86	3	8	198	1	186	1.02e-67	205
MS.gene33426.t1	AT5G49030	54.237	177	81	0	8	184	1	177	1.23e-59	201
MS.gene33426.t1	AT1G65870	52.318	151	72	0	47	197	39	189	2.79e-56	176
MS.gene33426.t1	AT2G21100	52.349	149	71	0	49	197	39	187	3.18e-55	174
MS.gene33426.t1	AT2G21100	52.349	149	71	0	49	197	39	187	3.18e-55	174
MS.gene33426.t1	AT3G13662	55.705	149	63	1	37	185	27	172	2.19e-54	171
MS.gene33426.t1	AT3G13660	63.559	118	43	0	81	198	8	125	2.08e-51	162
MS.gene33426.t1	AT1G22900	50.993	151	72	1	47	197	45	193	1.76e-50	162
MS.gene33426.t1	AT5G42500	49.669	151	72	2	48	197	38	185	1.92e-48	156
MS.gene33426.t1	AT5G42510	48.344	151	74	2	48	197	35	182	2.63e-47	153
MS.gene33426.t1	AT4G38700	44.872	156	80	3	49	198	35	190	6.53e-41	137
MS.gene33426.t1	AT2G21110	38.824	170	94	2	33	198	23	186	2.45e-38	130
MS.gene33426.t1	AT3G58090	52.727	110	52	0	88	197	162	271	3.17e-38	133
MS.gene33426.t1	AT4G11210	28.729	181	114	4	1	181	1	166	1.79e-16	74.3
MS.gene33426.t1	AT4G23690	36.364	132	77	3	46	173	34	162	3.17e-16	73.6
MS.gene33426.t1	AT4G11180	29.703	202	118	7	1	197	1	183	7.52e-12	61.6

Find 37 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTTCTGGTCCTAAAGTTAA+AGG	0.191161	4.2:-87075973	None:intergenic
CATTGGATTGATTTCAAAAC+TGG	0.225984	4.2:+87076222	MS.gene33426:CDS
ATTGACAACCCTTTAACTTT+AGG	0.265955	4.2:+87075964	MS.gene33426:CDS
TTCATTGAAGGAAAGTATAA+TGG	0.275934	4.2:+87076084	MS.gene33426:CDS
TCTGCTTCACAAGAAGAAAT+AGG	0.302577	4.2:+87076036	MS.gene33426:CDS
CATCTCCCTAACCTTATTAA+TGG	0.302823	4.2:-87076139	None:intergenic
CCAATAGATCGCAAGTTGTT+AGG	0.329140	4.2:+87075802	MS.gene33426:CDS
CCTAACAACTTGCGATCTAT+TGG	0.333660	4.2:-87075802	None:intergenic
GTAAACTCAACCACTGCTTT+TGG	0.339769	4.2:+87075928	MS.gene33426:CDS
TAAGTCATTTCAGATTCTAT+TGG	0.351815	4.2:+87075845	MS.gene33426:CDS
GAGGAATCCTGCCATTAATA+AGG	0.372389	4.2:+87076128	MS.gene33426:CDS
ATGCCTGTTGTTGGTGGTAG+TGG	0.375988	4.2:+87076159	MS.gene33426:CDS
GAACTGAGTTCTAAACTTGT+TGG	0.385159	4.2:+87075991	MS.gene33426:CDS
GGGAGTACTCTTACTATTGT+AGG	0.399599	4.2:+87076105	MS.gene33426:CDS
GTTCTGGTCCTAAAGTTAAA+GGG	0.426776	4.2:-87075972	None:intergenic
ACAAGTTTAGAACTCAGTTC+TGG	0.427865	4.2:-87075988	None:intergenic
GGAACAATTGCAACCGAAGT+TGG	0.428664	4.2:-87075892	None:intergenic
TCATTGAAGGAAAGTATAAT+GGG	0.435892	4.2:+87076085	MS.gene33426:CDS
ACAAAGTCATGATGAAATGT+TGG	0.438715	4.2:-87075699	None:intergenic
ATGCTCAAGCTAATACTCAT+TGG	0.456460	4.2:+87076205	MS.gene33426:CDS
TCCCTAACCTTATTAATGGC+AGG	0.465870	4.2:-87076135	None:intergenic
CAAAGTCATGATGAAATGTT+GGG	0.470092	4.2:-87075698	None:intergenic
AAACTTGTTGGAAGAGCTCA+AGG	0.474626	4.2:+87076003	MS.gene33426:CDS
ATCCTGCCATTAATAAGGTT+AGG	0.495140	4.2:+87076133	MS.gene33426:CDS
TCCTGCCATTAATAAGGTTA+GGG	0.498540	4.2:+87076134	MS.gene33426:CDS
GTTAGGGAGATGCCTGTTGT+TGG	0.505285	4.2:+87076150	MS.gene33426:CDS
AGTCCACTACCACCAACAAC+AGG	0.512977	4.2:-87076162	None:intergenic
AGGGAGATGCCTGTTGTTGG+TGG	0.521357	4.2:+87076153	MS.gene33426:CDS
GGAGTACTCTTACTATTGTA+GGG	0.530187	4.2:+87076106	MS.gene33426:CDS
TATTGGCATGACATCTTGAG+TGG	0.547551	4.2:+87075862	MS.gene33426:CDS
GTCATGATGAAATGTTGGGA+AGG	0.583236	4.2:-87075694	None:intergenic
ATTGGATTGATTTCAAAACT+GGG	0.602248	4.2:+87076223	MS.gene33426:CDS
TTGGATTGATTTCAAAACTG+GGG	0.621102	4.2:+87076224	MS.gene33426:CDS
GTGTGTTGCAAGATATGAGA+AGG	0.624292	4.2:-87075729	None:intergenic
GTTGACTAAACCAAAAGCAG+TGG	0.670239	4.2:-87075938	None:intergenic
GTACTCTTACTATTGTAGGG+AGG	0.687219	4.2:+87076109	MS.gene33426:CDS
AACTTGTTGGAAGAGCTCAA+GGG	0.691566	4.2:+87076004	MS.gene33426:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TAAGTCATTTCAGATTCTAT+TGG	+	chr4.2:87075845-87075864	MS.gene33426:CDS	25.0%
!!	ATGAATTTTGCTTTCATTGA+AGG	+	chr4.2:87076072-87076091	MS.gene33426:CDS	25.0%
!!	ATTGGATTGATTTCAAAACT+GGG	+	chr4.2:87076223-87076242	MS.gene33426:CDS	25.0%
!!	TCATTGAAGGAAAGTATAAT+GGG	+	chr4.2:87076085-87076104	MS.gene33426:CDS	25.0%
!!	TTCATTGAAGGAAAGTATAA+TGG	+	chr4.2:87076084-87076103	MS.gene33426:CDS	25.0%
	ACAAAGTCATGATGAAATGT+TGG	-	chr4.2:87075702-87075721	None:intergenic	30.0%
	CAAAGTCATGATGAAATGTT+GGG	-	chr4.2:87075701-87075720	None:intergenic	30.0%
!	ATTGACAACCCTTTAACTTT+AGG	+	chr4.2:87075964-87075983	MS.gene33426:CDS	30.0%
!	CATTGGATTGATTTCAAAAC+TGG	+	chr4.2:87076222-87076241	MS.gene33426:CDS	30.0%
!	TTGGATTGATTTCAAAACTG+GGG	+	chr4.2:87076224-87076243	MS.gene33426:CDS	30.0%
!!	GTTGAGTTTACTTTTGATGA+TGG	-	chr4.2:87075919-87075938	None:intergenic	30.0%
	ATCCTGCCATTAATAAGGTT+AGG	+	chr4.2:87076133-87076152	MS.gene33426:CDS	35.0%
	ATGCTCAAGCTAATACTCAT+TGG	+	chr4.2:87076205-87076224	MS.gene33426:CDS	35.0%
	CATCTCCCTAACCTTATTAA+TGG	-	chr4.2:87076142-87076161	None:intergenic	35.0%
	GAGTGGAAAAAATCCAACTT+CGG	+	chr4.2:87075879-87075898	MS.gene33426:CDS	35.0%
	GTTCTGGTCCTAAAGTTAAA+GGG	-	chr4.2:87075975-87075994	None:intergenic	35.0%
	TCCTGCCATTAATAAGGTTA+GGG	+	chr4.2:87076134-87076153	MS.gene33426:CDS	35.0%
	TCTGCTTCACAAGAAGAAAT+AGG	+	chr4.2:87076036-87076055	MS.gene33426:CDS	35.0%
!	ACAAGTTTAGAACTCAGTTC+TGG	-	chr4.2:87075991-87076010	None:intergenic	35.0%
!	AGTTCTGGTCCTAAAGTTAA+AGG	-	chr4.2:87075976-87075995	None:intergenic	35.0%
!	GGAGTACTCTTACTATTGTA+GGG	+	chr4.2:87076106-87076125	MS.gene33426:CDS	35.0%
!!	GAACTGAGTTCTAAACTTGT+TGG	+	chr4.2:87075991-87076010	MS.gene33426:CDS	35.0%
!!!	GAGTTTACTTTTGATGATGG+TGG	-	chr4.2:87075916-87075935	None:intergenic	35.0%
	CCAATAGATCGCAAGTTGTT+AGG	+	chr4.2:87075802-87075821	MS.gene33426:CDS	40.0%
	CCTAACAACTTGCGATCTAT+TGG	-	chr4.2:87075805-87075824	None:intergenic	40.0%
	GAGGAATCCTGCCATTAATA+AGG	+	chr4.2:87076128-87076147	MS.gene33426:CDS	40.0%
	GTACTCTTACTATTGTAGGG+AGG	+	chr4.2:87076109-87076128	MS.gene33426:CDS	40.0%
	GTCATGATGAAATGTTGGGA+AGG	-	chr4.2:87075697-87075716	None:intergenic	40.0%
	GTGTGTTGCAAGATATGAGA+AGG	-	chr4.2:87075732-87075751	None:intergenic	40.0%
	GTTGACTAAACCAAAAGCAG+TGG	-	chr4.2:87075941-87075960	None:intergenic	40.0%
	TATTGGCATGACATCTTGAG+TGG	+	chr4.2:87075862-87075881	MS.gene33426:CDS	40.0%
	TCCCTAACCTTATTAATGGC+AGG	-	chr4.2:87076138-87076157	None:intergenic	40.0%
!	GTAAACTCAACCACTGCTTT+TGG	+	chr4.2:87075928-87075947	MS.gene33426:CDS	40.0%
!!	AAACTTGTTGGAAGAGCTCA+AGG	+	chr4.2:87076003-87076022	MS.gene33426:CDS	40.0%
!!	AACTTGTTGGAAGAGCTCAA+GGG	+	chr4.2:87076004-87076023	MS.gene33426:CDS	40.0%
!!	GGGAGTACTCTTACTATTGT+AGG	+	chr4.2:87076105-87076124	MS.gene33426:CDS	40.0%
!!!	GGACTTTTCAGATTTGCTAG+AGG	+	chr4.2:87076180-87076199	MS.gene33426:CDS	40.0%
	GGAACAATTGCAACCGAAGT+TGG	-	chr4.2:87075895-87075914	None:intergenic	45.0%
	AGTCCACTACCACCAACAAC+AGG	-	chr4.2:87076165-87076184	None:intergenic	50.0%
	GTTAGGGAGATGCCTGTTGT+TGG	+	chr4.2:87076150-87076169	MS.gene33426:CDS	50.0%
!!	ATGCCTGTTGTTGGTGGTAG+TGG	+	chr4.2:87076159-87076178	MS.gene33426:CDS	50.0%
	AGGGAGATGCCTGTTGTTGG+TGG	+	chr4.2:87076153-87076172	MS.gene33426:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	87075691	87076287	87075691	ID=MS.gene33426
chr4.2	mRNA	87075691	87076287	87075691	ID=MS.gene33426.t1;Parent=MS.gene33426
chr4.2	exon	87075691	87076287	87075691	ID=MS.gene33426.t1.exon1;Parent=MS.gene33426.t1
chr4.2	CDS	87075691	87076287	87075691	ID=cds.MS.gene33426.t1;Parent=MS.gene33426.t1

Gene Sequence

>MS.gene33426

ATGCCTTCCCAACATTTCATCATGACTTTGTTCTTCTTCCTTCTCATATCTTGCAACACACTCACTGCTTCAACTTCGTCAGAAAAGCAAGACACTTTCGATTTTGTTCGTCCAATAGATCGCAAGTTGTTAGGCTTAAACAAGAAAGAAAAGCTAAGTCATTTCAGATTCTATTGGCATGACATCTTGAGTGGAAAAAATCCAACTTCGGTTGCAATTGTTCCACCATCATCAAAAGTAAACTCAACCACTGCTTTTGGTTTAGTCAACATGATTGACAACCCTTTAACTTTAGGACCAGAACTGAGTTCTAAACTTGTTGGAAGAGCTCAAGGGTTCTATGCATCTGCTTCACAAGAAGAAATAGGTTTTCTTATGACAATGAATTTTGCTTTCATTGAAGGAAAGTATAATGGGAGTACTCTTACTATTGTAGGGAGGAATCCTGCCATTAATAAGGTTAGGGAGATGCCTGTTGTTGGTGGTAGTGGACTTTTCAGATTTGCTAGAGGATATGCTCAAGCTAATACTCATTGGATTGATTTCAAAACTGGGGATGCTTGTGTTGAGTACAATGTCCATGTTTTTCACTATTGA

Protein sequence

>MS.gene33426.t1

MPSQHFIMTLFFFLLISCNTLTASTSSEKQDTFDFVRPIDRKLLGLNKKEKLSHFRFYWHDILSGKNPTSVAIVPPSSKVNSTTAFGLVNMIDNPLTLGPELSSKLVGRAQGFYASASQEEIGFLMTMNFAFIEGKYNGSTLTIVGRNPAINKVREMPVVGGSGLFRFARGYAQANTHWIDFKTGDACVEYNVHVFHY