AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068551

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068551.t1 MTR_1g067560 95.019 261 11 1 1 259 1 261 9.80e-175 481
MS.gene068551.t1 MTR_7g100450 57.875 273 89 8 1 247 1 273 1.03e-85 257
MS.gene068551.t1 MTR_7g100450 52.966 236 85 8 1 210 1 236 4.84e-59 187
MS.gene068551.t1 MTR_4g062450 39.216 255 105 9 1 246 1 214 2.63e-41 142
MS.gene068551.t1 MTR_6g086020 56.250 64 28 0 183 246 65 128 7.45e-23 91.3
MS.gene068551.t1 MTR_8g103820 56.250 64 28 0 183 246 66 129 1.67e-22 90.1
MS.gene068551.t1 MTR_7g013660 44.776 67 36 1 181 246 2 68 2.24e-14 68.6
MS.gene068551.t1 MTR_1g092670 42.029 69 39 1 181 248 2 70 6.72e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068551.t1 AT2G37390 42.857 252 120 9 6 246 5 243 4.75e-51 167
MS.gene068551.t1 AT2G37390 42.292 253 121 9 6 246 5 244 1.19e-49 164
MS.gene068551.t1 AT3G53530 41.569 255 118 9 1 246 1 233 2.54e-46 155
MS.gene068551.t1 AT3G53530 41.406 256 118 10 1 246 1 234 1.64e-44 150
MS.gene068551.t1 AT5G02600 78.082 73 16 0 177 249 244 316 5.42e-35 128
MS.gene068551.t1 AT5G02600 78.082 73 16 0 177 249 244 316 5.42e-35 128
MS.gene068551.t1 AT2G28660 73.438 64 17 0 183 246 183 246 4.49e-27 105
MS.gene068551.t1 AT3G24450 51.562 64 31 0 183 246 74 137 9.69e-18 77.8
MS.gene068551.t1 AT1G66240 43.038 79 44 1 177 254 27 105 4.60e-16 72.4
MS.gene068551.t1 AT3G56240 43.284 67 37 1 182 247 2 68 2.72e-14 68.2
MS.gene068551.t1 AT3G56240 43.284 67 37 1 182 247 2 68 2.72e-14 68.2
MS.gene068551.t1 AT5G27690 46.032 63 33 1 186 247 33 95 1.83e-12 66.6

Find 51 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGAATCATTGATAAT+AGG 0.154536 1.2:-50966261 None:intergenic
TCCTCCTAATTAGATGAATT+TGG 0.164221 1.2:-50967482 None:intergenic
TTAGATGAATTTGGTTTCTT+TGG 0.205681 1.2:-50967473 None:intergenic
TTCTTGAATCATTGATAATA+GGG 0.260643 1.2:-50966260 None:intergenic
GGAGTAATTGAGTCAGAATT+TGG 0.263951 1.2:-50966501 None:intergenic
ATGCTTGCCAATACACTAAA+TGG 0.274222 1.2:-50967403 None:intergenic
AGGTGAAGAATGCACAATTT+TGG 0.279496 1.2:+50967431 MS.gene068551:CDS
AACTAAGGTTGATGATAAAA+AGG 0.295490 1.2:+50966602 MS.gene068551:CDS
ATCCAACCCTGCATCTAAAT+CGG 0.376694 1.2:+50966647 MS.gene068551:CDS
TTCCATTCTGAAAAGGAGAT+TGG 0.404756 1.2:+50966473 MS.gene068551:CDS
TCTTCTTCTTCCAAGCCAAT+TGG 0.409571 1.2:+50966357 MS.gene068551:CDS
GCAAAGAAGGTGACAATAGT+TGG 0.411060 1.2:+50967370 MS.gene068551:CDS
TTCTGTCTTGGCATTAACTA+AGG 0.413181 1.2:+50966587 MS.gene068551:CDS
TGTGACTCCATTTAGTGTAT+TGG 0.414736 1.2:+50967396 MS.gene068551:CDS
CATGTTCTGTCCAATTGGCT+TGG 0.427254 1.2:-50966367 None:intergenic
TTGAGCAGGTTGTAGTATTG+AGG 0.431624 1.2:+50967038 MS.gene068551:intron
CAAAGAAGGTGACAATAGTT+GGG 0.432731 1.2:+50967371 MS.gene068551:CDS
TTCGAATTATGATTCTGTCT+TGG 0.443038 1.2:+50966575 MS.gene068551:CDS
TGCAGCAAAATCTATGTTGA+AGG 0.445020 1.2:-50967350 None:intergenic
GACTTTGCCTCCAAGTTGAA+TGG 0.449627 1.2:-50966223 None:intergenic
TGAGCAGGTTGTAGTATTGA+GGG 0.452176 1.2:+50967039 MS.gene068551:intron
GAGGAACCCGATTTAGATGC+AGG 0.453934 1.2:-50966654 None:intergenic
GGGTTTCTTTGCATTGCAAA+GGG 0.475577 1.2:+50967059 MS.gene068551:CDS
TCAAATACCATTCAACTTGG+AGG 0.476391 1.2:+50966216 MS.gene068551:CDS
TCCAACCCTGCATCTAAATC+GGG 0.488661 1.2:+50966648 MS.gene068551:CDS
CAATTGATTCCATTCTGAAA+AGG 0.489678 1.2:+50966466 MS.gene068551:CDS
AATCATTGATAATAGGGTTG+TGG 0.501645 1.2:-50966254 None:intergenic
ACAAGGAGCAGTGATAGTCT+TGG 0.502397 1.2:-50966292 None:intergenic
TCTTCAAATACCATTCAACT+TGG 0.505844 1.2:+50966213 MS.gene068551:CDS
AGGGTTTCTTTGCATTGCAA+AGG 0.507191 1.2:+50967058 MS.gene068551:CDS
TATCCAAAAGATGAAAAGAA+TGG 0.508564 1.2:+50966125 None:intergenic
ACCCGATTTAGATGCAGGGT+TGG 0.510035 1.2:-50966649 None:intergenic
TTGGTGTCAGATCCAAGTGC+TGG 0.516428 1.2:-50967454 None:intergenic
TGCATTGCAAAGGGTGTGAA+GGG 0.518392 1.2:+50967068 MS.gene068551:CDS
TTGCATTGCAAAGGGTGTGA+AGG 0.536259 1.2:+50967067 MS.gene068551:CDS
GCTAAGACATATTGCTGTTG+AGG 0.541448 1.2:-50966169 None:intergenic
TGGCTTGGAAGAAGAAGAAG+TGG 0.558993 1.2:-50966352 None:intergenic
AACAGCAATATGTCTTAGCA+TGG 0.568544 1.2:+50966173 MS.gene068551:CDS
TAAATCGGGTTCCTCAAGCC+AGG 0.574328 1.2:+50966662 MS.gene068551:CDS
AGGAACCCGATTTAGATGCA+GGG 0.591898 1.2:-50966653 None:intergenic
AAACACATCTCTAAAATGCA+AGG 0.597452 1.2:+50967100 MS.gene068551:CDS
TGTATTGGCAAGCATATCAA+AGG 0.601005 1.2:+50967411 MS.gene068551:CDS
TGGCTTAGAAGAGTAACTGT+TGG 0.609262 1.2:-50966445 None:intergenic
AGCAAAATCTATGTTGAAGG+AGG 0.618997 1.2:-50967347 None:intergenic
GGTAGCTGAGAAGAAGAACA+AGG 0.627415 1.2:-50966309 None:intergenic
TCTGACTCAATTACTCCACA+TGG 0.643353 1.2:+50966507 MS.gene068551:CDS
AAGAAAACTAACCTGGCTTG+AGG 0.646869 1.2:-50966673 None:intergenic
AAGGGTGTGAAGGGAAGCTG+AGG 0.652756 1.2:+50967077 MS.gene068551:CDS
AAAGAAGGTGACAATAGTTG+GGG 0.656060 1.2:+50967372 MS.gene068551:CDS
GCAAAAGAAGAAAAGTACAG+TGG 0.667428 1.2:+50966413 MS.gene068551:CDS
AAAGATCTAGTTGAACCATG+TGG 0.741520 1.2:-50966522 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATACATTTTTTAAATGT+GGG - chr1.2:50967140-50967159 None:intergenic 10.0%
!!! TTAAATACATTTTTTAAATG+TGG - chr1.2:50967141-50967160 None:intergenic 10.0%
!! AGTTAAAGATTATTTAAAGA+TGG - chr1.2:50967227-50967246 None:intergenic 15.0%
!!! AAATACATTTTTTAAATGTG+GGG - chr1.2:50967139-50967158 None:intergenic 15.0%
!!! TTTAATCTTGTTGATTTTAT+GGG + chr1.2:50967315-50967334 MS.gene068551:intron 15.0%
!!! TTTTAATCTTGTTGATTTTA+TGG + chr1.2:50967314-50967333 MS.gene068551:intron 15.0%
!! AAAATTCATAAAACTAGTCA+TGG - chr1.2:50966992-50967011 None:intergenic 20.0%
!! TAAATGCAGAAAATTAAGAT+TGG - chr1.2:50966879-50966898 None:intergenic 20.0%
!!! TTATTTTTTGCCACTTATTT+AGG + chr1.2:50966848-50966867 MS.gene068551:intron 20.0%
!!! TTCTTGAATCATTGATAATA+GGG - chr1.2:50966263-50966282 None:intergenic 20.0%
! ACATTCTCTCAAATTATTAC+CGG + chr1.2:50966775-50966794 MS.gene068551:intron 25.0%
! ATTGGAAAATCCTAAATAAG+TGG - chr1.2:50966861-50966880 None:intergenic 25.0%
! ATTTGAGTAAATATTTGAGC+AGG + chr1.2:50967024-50967043 MS.gene068551:intron 25.0%
! TCTCATATTTGAATTCCATT+CGG - chr1.2:50967294-50967313 None:intergenic 25.0%
!! CTTCTTGAATCATTGATAAT+AGG - chr1.2:50966264-50966283 None:intergenic 25.0%
!! TTAGATGAATTTGGTTTCTT+TGG - chr1.2:50967476-50967495 None:intergenic 25.0%
!!! AACTAAGGTTGATGATAAAA+AGG + chr1.2:50966602-50966621 MS.gene068551:CDS 25.0%
ATTGGACAGAACATGAAAAA+TGG + chr1.2:50966375-50966394 MS.gene068551:CDS 30.0%
GAATATGAACTAAGCAAGTT+TGG + chr1.2:50966945-50966964 MS.gene068551:intron 30.0%
GTGGCAAAAAATAAAACCTA+TGG - chr1.2:50966842-50966861 None:intergenic 30.0%
TCTTCAAATACCATTCAACT+TGG + chr1.2:50966213-50966232 MS.gene068551:CDS 30.0%
TTCGAATTATGATTCTGTCT+TGG + chr1.2:50966575-50966594 MS.gene068551:CDS 30.0%
! AATCATTGATAATAGGGTTG+TGG - chr1.2:50966257-50966276 None:intergenic 30.0%
! CAATTGATTCCATTCTGAAA+AGG + chr1.2:50966466-50966485 MS.gene068551:CDS 30.0%
! CTTTTCAGAATGGAATCAAT+TGG - chr1.2:50966468-50966487 None:intergenic 30.0%
! GATAAAAAGGATCAAGTTGT+TGG + chr1.2:50966615-50966634 MS.gene068551:CDS 30.0%
! TTTTTCATGTTCTGTCCAAT+TGG - chr1.2:50966375-50966394 None:intergenic 30.0%
!! AAACACATCTCTAAAATGCA+AGG + chr1.2:50967100-50967119 MS.gene068551:CDS 30.0%
AAAGAAGGTGACAATAGTTG+GGG + chr1.2:50967372-50967391 MS.gene068551:CDS 35.0%
AAAGATCTAGTTGAACCATG+TGG - chr1.2:50966525-50966544 None:intergenic 35.0%
AACAGCAATATGTCTTAGCA+TGG + chr1.2:50966173-50966192 MS.gene068551:CDS 35.0%
ACGAGAAAAGAAAACTAACC+TGG - chr1.2:50966683-50966702 None:intergenic 35.0%
AGCAAAATCTATGTTGAAGG+AGG - chr1.2:50967350-50967369 None:intergenic 35.0%
ATGCTTGCCAATACACTAAA+TGG - chr1.2:50967406-50967425 None:intergenic 35.0%
CAAAGAAGGTGACAATAGTT+GGG + chr1.2:50967371-50967390 MS.gene068551:CDS 35.0%
GACACTTTAAATTGGAAGGT+GGG + chr1.2:50966709-50966728 MS.gene068551:intron 35.0%
GGAGTAATTGAGTCAGAATT+TGG - chr1.2:50966504-50966523 None:intergenic 35.0%
GTAACACCGACACTTTAAAT+TGG + chr1.2:50966701-50966720 MS.gene068551:intron 35.0%
TATGTCACACATTTACCGAA+TGG + chr1.2:50967276-50967295 MS.gene068551:intron 35.0%
TCAAATACCATTCAACTTGG+AGG + chr1.2:50966216-50966235 MS.gene068551:CDS 35.0%
TGCAGCAAAATCTATGTTGA+AGG - chr1.2:50967353-50967372 None:intergenic 35.0%
TGTGACTCCATTTAGTGTAT+TGG + chr1.2:50967396-50967415 MS.gene068551:CDS 35.0%
TTCTGTCTTGGCATTAACTA+AGG + chr1.2:50966587-50966606 MS.gene068551:CDS 35.0%
! AACCAATCTCCTTTTCAGAA+TGG - chr1.2:50966478-50966497 None:intergenic 35.0%
! AAGAAGTGGTTTTCTTCTTG+GGG - chr1.2:50966341-50966360 None:intergenic 35.0%
! AGAAGAAGTGGTTTTCTTCT+TGG - chr1.2:50966343-50966362 None:intergenic 35.0%
! AGGTGAAGAATGCACAATTT+TGG + chr1.2:50967431-50967450 MS.gene068551:CDS 35.0%
! CACCTTCCAATTTAAAGTGT+CGG - chr1.2:50966710-50966729 None:intergenic 35.0%
! CATAGATTTTGCTGCAAAGA+AGG + chr1.2:50967357-50967376 MS.gene068551:CDS 35.0%
! CTGCATTTATTGCTTGCTAA+GGG + chr1.2:50966890-50966909 MS.gene068551:intron 35.0%
! GAAGAAGTGGTTTTCTTCTT+GGG - chr1.2:50966342-50966361 None:intergenic 35.0%
! GCAAAAGAAGAAAAGTACAG+TGG + chr1.2:50966413-50966432 MS.gene068551:CDS 35.0%
! TCTGCATTTATTGCTTGCTA+AGG + chr1.2:50966889-50966908 MS.gene068551:intron 35.0%
! TGTATTGGCAAGCATATCAA+AGG + chr1.2:50967411-50967430 MS.gene068551:CDS 35.0%
! TTCCATTCTGAAAAGGAGAT+TGG + chr1.2:50966473-50966492 MS.gene068551:CDS 35.0%
!! CATCTCTAAAATGCAAGGTA+TGG + chr1.2:50967105-50967124 MS.gene068551:intron 35.0%
!!! ATCTTGTTGATTTTATGGGC+AGG + chr1.2:50967319-50967338 MS.gene068551:intron 35.0%
AGGGTTTCTTTGCATTGCAA+AGG + chr1.2:50967058-50967077 MS.gene068551:CDS 40.0%
ATCCAACCCTGCATCTAAAT+CGG + chr1.2:50966647-50966666 MS.gene068551:CDS 40.0%
CACCGACACTTTAAATTGGA+AGG + chr1.2:50966705-50966724 MS.gene068551:intron 40.0%
CGACACTTTAAATTGGAAGG+TGG + chr1.2:50966708-50966727 MS.gene068551:intron 40.0%
GCAAAGAAGGTGACAATAGT+TGG + chr1.2:50967370-50967389 MS.gene068551:CDS 40.0%
GGGTTTCTTTGCATTGCAAA+GGG + chr1.2:50967059-50967078 MS.gene068551:CDS 40.0%
TCTGACTCAATTACTCCACA+TGG + chr1.2:50966507-50966526 MS.gene068551:CDS 40.0%
TCTTCTTCTTCCAAGCCAAT+TGG + chr1.2:50966357-50966376 MS.gene068551:CDS 40.0%
TGGCTTAGAAGAGTAACTGT+TGG - chr1.2:50966448-50966467 None:intergenic 40.0%
TTGAGCAGGTTGTAGTATTG+AGG + chr1.2:50967038-50967057 MS.gene068551:intron 40.0%
TTGGACACATTCAACACATG+TGG + chr1.2:50966750-50966769 MS.gene068551:intron 40.0%
! AAGAAAACTAACCTGGCTTG+AGG - chr1.2:50966676-50966695 None:intergenic 40.0%
! GCTAAGACATATTGCTGTTG+AGG - chr1.2:50966172-50966191 None:intergenic 40.0%
! GTTTTCTTCTTGGGGTCAAT+AGG - chr1.2:50966333-50966352 None:intergenic 40.0%
! TGAGCAGGTTGTAGTATTGA+GGG + chr1.2:50967039-50967058 MS.gene068551:intron 40.0%
ACAAGGAGCAGTGATAGTCT+TGG - chr1.2:50966295-50966314 None:intergenic 45.0%
AGGAACCCGATTTAGATGCA+GGG - chr1.2:50966656-50966675 None:intergenic 45.0%
CATGTTCTGTCCAATTGGCT+TGG - chr1.2:50966370-50966389 None:intergenic 45.0%
GGTAGCTGAGAAGAAGAACA+AGG - chr1.2:50966312-50966331 None:intergenic 45.0%
GTCTAGTGTCAATGCTCCAT+AGG + chr1.2:50966823-50966842 MS.gene068551:intron 45.0%
TCCAACCCTGCATCTAAATC+GGG + chr1.2:50966648-50966667 MS.gene068551:CDS 45.0%
TGAATGTGTCCAACCTGTGT+TGG - chr1.2:50966743-50966762 None:intergenic 45.0%
! AGTGACGCAGCTTTTGTAGA+TGG + chr1.2:50966549-50966568 MS.gene068551:CDS 45.0%
! GACTTTGCCTCCAAGTTGAA+TGG - chr1.2:50966226-50966245 None:intergenic 45.0%
! TGCATTGCAAAGGGTGTGAA+GGG + chr1.2:50967068-50967087 MS.gene068551:CDS 45.0%
! TGGCTTGGAAGAAGAAGAAG+TGG - chr1.2:50966355-50966374 None:intergenic 45.0%
! TTACCGGTGTCAATGAGAAG+TGG + chr1.2:50966791-50966810 MS.gene068551:intron 45.0%
! TTGCATTGCAAAGGGTGTGA+AGG + chr1.2:50967067-50967086 MS.gene068551:CDS 45.0%
! TTGCCACTTCTCATTGACAC+CGG - chr1.2:50966797-50966816 None:intergenic 45.0%
ACCCGATTTAGATGCAGGGT+TGG - chr1.2:50966652-50966671 None:intergenic 50.0%
GAGGAACCCGATTTAGATGC+AGG - chr1.2:50966657-50966676 None:intergenic 50.0%
TAAATCGGGTTCCTCAAGCC+AGG + chr1.2:50966662-50966681 MS.gene068551:CDS 50.0%
!! GCACAATTTTGGCCAGCACT+TGG + chr1.2:50967442-50967461 MS.gene068551:CDS 50.0%
!! TTGGTGTCAGATCCAAGTGC+TGG - chr1.2:50967457-50967476 None:intergenic 50.0%
GTCCAACCTGTGTTGGACAC+TGG - chr1.2:50966736-50966755 None:intergenic 55.0%
GTCCAGTGTCCAACACAGGT+TGG + chr1.2:50966731-50966750 MS.gene068551:intron 55.0%
! AAGGGTGTGAAGGGAAGCTG+AGG + chr1.2:50967077-50967096 MS.gene068551:CDS 55.0%
GTGGGTCCAGTGTCCAACAC+AGG + chr1.2:50966727-50966746 MS.gene068551:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 50966135 50967495 50966135 ID=MS.gene068551
chr1.2 mRNA 50966135 50967495 50966135 ID=MS.gene068551.t1;Parent=MS.gene068551
chr1.2 exon 50966135 50966683 50966135 ID=MS.gene068551.t1.exon1;Parent=MS.gene068551.t1
chr1.2 CDS 50966135 50966683 50966135 ID=cds.MS.gene068551.t1;Parent=MS.gene068551.t1
chr1.2 exon 50967046 50967121 50967046 ID=MS.gene068551.t1.exon2;Parent=MS.gene068551.t1
chr1.2 CDS 50967046 50967121 50967046 ID=cds.MS.gene068551.t1;Parent=MS.gene068551.t1
chr1.2 exon 50967341 50967495 50967341 ID=MS.gene068551.t1.exon3;Parent=MS.gene068551.t1
chr1.2 CDS 50967341 50967495 50967341 ID=cds.MS.gene068551.t1;Parent=MS.gene068551.t1
Gene Sequence

>MS.gene068551

ATGAAAAGAATGGATATCTTCTGTGCTTCACAAGCCTCAACAGCAATATGTCTTAGCATGGAACAAGCTTCATGCTCTTCTTCAAATACCATTCAACTTGGAGGCAAAGTCATTGATCGCCACAACCCTATTATCAATGATTCAAGAAGAAGCACTTCCAAGACTATCACTGCTCCTTGTTCTTCTTCTCAGCTACCTATTGACCCCAAGAAGAAAACCACTTCTTCTTCTTCCAAGCCAATTGGACAGAACATGAAAAATGGAGCTAAAGTTTATGAGCAAAAGAAGAAAAGTACAGTGGAAAATGTCACCAACAGTTACTCTTCTAAGCCAATTGATTCCATTCTGAAAAGGAGATTGGTTAAGCCAAATTCTGACTCAATTACTCCACATGGTTCAACTAGATCTTTGCTCAGTGACGCAGCTTTTGTAGATGGCTCTTCGAATTATGATTCTGTCTTGGCATTAACTAAGGTTGATGATAAAAAGGATCAAGTTGTTGGTCAAAATGAATCCAACCCTGCATCTAAATCGGGTTCCTCAAGCCAGGTTAGTTTTCTTTTCTCGTAACACCGACACTTTAAATTGGAAGGTGGGTCCAGTGTCCAACACAGGTTGGACACATTCAACACATGTGGTTACATTCTCTCAAATTATTACCGGTGTCAATGAGAAGTGGCAATGTCGTGTCTAGTGTCAATGCTCCATAGGTTTTATTTTTTGCCACTTATTTAGGATTTTCCAATCTTAATTTTCTGCATTTATTGCTTGCTAAGGGCTCTCTAATTGTAAATTTATGATATGCTTCGTGAATATGAACTAAGCAAGTTTGGAAGCATATGTTTGTAGAATAACCATGACTAGTTTTATGAATTTTCACTAATTGATGATTTGAGTAAATATTTGAGCAGGTTGTAGTATTGAGGGTTTCTTTGCATTGCAAAGGGTGTGAAGGGAAGCTGAGGAAACACATCTCTAAAATGCAAGGTATGGACTTGATTCCCCACATTTAAAAAATGTATTTAAACGCGCTAATTGATCTCGATCATCAATTTGAGATCAATCAATTCACGTGAAATCTTCACGAAACCATCTTTAAATAATCTTTAACTGTACAATTTAAAATCGAAAAGTCAATAAATATGTCACACATTTACCGAATGGAATTCAAATATGAGATTTTAATCTTGTTGATTTTATGGGCAGGAGTTACCTCCTTCAACATAGATTTTGCTGCAAAGAAGGTGACAATAGTTGGGGATGTGACTCCATTTAGTGTATTGGCAAGCATATCAAAGGTGAAGAATGCACAATTTTGGCCAGCACTTGGATCTGACACCAAAGAAACCAAATTCATCTAA
Protein sequence

>MS.gene068551.t1

MKRMDIFCASQASTAICLSMEQASCSSSNTIQLGGKVIDRHNPIINDSRRSTSKTITAPCSSSQLPIDPKKKTTSSSSKPIGQNMKNGAKVYEQKKKSTVENVTNSYSSKPIDSILKRRLVKPNSDSITPHGSTRSLLSDAAFVDGSSNYDSVLALTKVDDKKDQVVGQNESNPASKSGSSSQVVVLRVSLHCKGCEGKLRKHISKMQGVTSFNIDFAAKKVTIVGDVTPFSVLASISKVKNAQFWPALGSDTKETKFI