AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30857

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30857.t1 MTR_1g067560 95.019 261 11 1 1 259 1 261 9.80e-175 481
MS.gene30857.t1 MTR_7g100450 57.875 273 89 8 1 247 1 273 1.03e-85 257
MS.gene30857.t1 MTR_7g100450 52.966 236 85 8 1 210 1 236 4.84e-59 187
MS.gene30857.t1 MTR_4g062450 39.216 255 105 9 1 246 1 214 2.63e-41 142
MS.gene30857.t1 MTR_6g086020 56.250 64 28 0 183 246 65 128 7.45e-23 91.3
MS.gene30857.t1 MTR_8g103820 56.250 64 28 0 183 246 66 129 1.67e-22 90.1
MS.gene30857.t1 MTR_7g013660 44.776 67 36 1 181 246 2 68 2.24e-14 68.6
MS.gene30857.t1 MTR_1g092670 42.029 69 39 1 181 248 2 70 6.72e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30857.t1 AT2G37390 42.857 252 120 9 6 246 5 243 4.75e-51 167
MS.gene30857.t1 AT2G37390 42.292 253 121 9 6 246 5 244 1.19e-49 164
MS.gene30857.t1 AT3G53530 41.569 255 118 9 1 246 1 233 2.54e-46 155
MS.gene30857.t1 AT3G53530 41.406 256 118 10 1 246 1 234 1.64e-44 150
MS.gene30857.t1 AT5G02600 78.082 73 16 0 177 249 244 316 5.42e-35 128
MS.gene30857.t1 AT5G02600 78.082 73 16 0 177 249 244 316 5.42e-35 128
MS.gene30857.t1 AT2G28660 73.438 64 17 0 183 246 183 246 4.49e-27 105
MS.gene30857.t1 AT3G24450 51.562 64 31 0 183 246 74 137 9.69e-18 77.8
MS.gene30857.t1 AT1G66240 43.038 79 44 1 177 254 27 105 4.60e-16 72.4
MS.gene30857.t1 AT3G56240 43.284 67 37 1 182 247 2 68 2.72e-14 68.2
MS.gene30857.t1 AT3G56240 43.284 67 37 1 182 247 2 68 2.72e-14 68.2
MS.gene30857.t1 AT5G27690 46.032 63 33 1 186 247 33 95 1.83e-12 66.6

Find 51 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGAATCATTGATAAT+AGG 0.154536 1.1:-49733776 None:intergenic
TCCTCCTAATTAGATGAATT+TGG 0.164221 1.1:-49734997 None:intergenic
TTAGATGAATTTGGTTTCTT+TGG 0.205681 1.1:-49734988 None:intergenic
TTCTTGAATCATTGATAATA+GGG 0.260643 1.1:-49733775 None:intergenic
GGAGTAATTGAGTCAGAATT+TGG 0.263951 1.1:-49734016 None:intergenic
ATGCTTGCCAATACACTAAA+TGG 0.274222 1.1:-49734918 None:intergenic
AGGTGAAGAATGCACAATTT+TGG 0.279496 1.1:+49734946 MS.gene30857:CDS
AACTAAGGTTGATGATAAAA+AGG 0.295490 1.1:+49734117 MS.gene30857:CDS
ATCCAACCCTGCATCTAAAT+CGG 0.376694 1.1:+49734162 MS.gene30857:CDS
TTCCATTCTGAAAAGGAGAT+TGG 0.404756 1.1:+49733988 MS.gene30857:CDS
TCTTCTTCTTCCAAGCCAAT+TGG 0.409571 1.1:+49733872 MS.gene30857:CDS
GCAAAGAAGGTGACAATAGT+TGG 0.411060 1.1:+49734885 MS.gene30857:CDS
TTCTGTCTTGGCATTAACTA+AGG 0.413181 1.1:+49734102 MS.gene30857:CDS
TGTGACTCCATTTAGTGTAT+TGG 0.414736 1.1:+49734911 MS.gene30857:CDS
CATGTTCTGTCCAATTGGCT+TGG 0.427254 1.1:-49733882 None:intergenic
TTGAGCAGGTTGTAGTATTG+AGG 0.431624 1.1:+49734553 MS.gene30857:intron
CAAAGAAGGTGACAATAGTT+GGG 0.432731 1.1:+49734886 MS.gene30857:CDS
TTCGAATTATGATTCTGTCT+TGG 0.443038 1.1:+49734090 MS.gene30857:CDS
TGCAGCAAAATCTATGTTGA+AGG 0.445020 1.1:-49734865 None:intergenic
GACTTTGCCTCCAAGTTGAA+TGG 0.449627 1.1:-49733738 None:intergenic
TGAGCAGGTTGTAGTATTGA+GGG 0.452176 1.1:+49734554 MS.gene30857:intron
GAGGAACCCGATTTAGATGC+AGG 0.453934 1.1:-49734169 None:intergenic
GGGTTTCTTTGCATTGCAAA+GGG 0.475577 1.1:+49734574 MS.gene30857:CDS
TCCAACCCTGCATCTAAATC+GGG 0.488661 1.1:+49734163 MS.gene30857:CDS
CAATTGATTCCATTCTGAAA+AGG 0.489678 1.1:+49733981 MS.gene30857:CDS
AATCATTGATAATAGGGTTG+TGG 0.501645 1.1:-49733769 None:intergenic
ACAAGGAGCAGTGATAGTCT+TGG 0.502397 1.1:-49733807 None:intergenic
TCAAATACCATTCAACTTGG+AGG 0.505409 1.1:+49733731 MS.gene30857:CDS
AGGGTTTCTTTGCATTGCAA+AGG 0.507191 1.1:+49734573 MS.gene30857:CDS
TATCCAAAAGATGAAAAGAA+TGG 0.508564 1.1:+49733640 None:intergenic
ACCCGATTTAGATGCAGGGT+TGG 0.510035 1.1:-49734164 None:intergenic
TTGGTGTCAGATCCAAGTGC+TGG 0.516428 1.1:-49734969 None:intergenic
TGCATTGCAAAGGGTGTGAA+GGG 0.518392 1.1:+49734583 MS.gene30857:CDS
TCTTCAAATACCATTCAACT+TGG 0.534861 1.1:+49733728 MS.gene30857:CDS
TTGCATTGCAAAGGGTGTGA+AGG 0.536259 1.1:+49734582 MS.gene30857:CDS
GCTAAGACATATTGCTGTTG+AGG 0.541448 1.1:-49733684 None:intergenic
TGGCTTGGAAGAAGAAGAAG+TGG 0.558993 1.1:-49733867 None:intergenic
AACAGCAATATGTCTTAGCA+TGG 0.568544 1.1:+49733688 MS.gene30857:CDS
TAAATCGGGTTCCTCAAGCC+AGG 0.574328 1.1:+49734177 MS.gene30857:CDS
AGGAACCCGATTTAGATGCA+GGG 0.591898 1.1:-49734168 None:intergenic
AAACACATCTCTAAAATGCA+AGG 0.597452 1.1:+49734615 MS.gene30857:CDS
TGTATTGGCAAGCATATCAA+AGG 0.601005 1.1:+49734926 MS.gene30857:CDS
TGGCTTAGAAGAGTAACTGT+TGG 0.609262 1.1:-49733960 None:intergenic
AGCAAAATCTATGTTGAAGG+AGG 0.618997 1.1:-49734862 None:intergenic
GGTAGCTGAGAAGAAGAACA+AGG 0.627415 1.1:-49733824 None:intergenic
TCTGACTCAATTACTCCACA+TGG 0.643353 1.1:+49734022 MS.gene30857:CDS
AAGAAAACTAACCTGGCTTG+AGG 0.646869 1.1:-49734188 None:intergenic
AAGGGTGTGAAGGGAAGCTG+AGG 0.652756 1.1:+49734592 MS.gene30857:CDS
AAAGAAGGTGACAATAGTTG+GGG 0.656060 1.1:+49734887 MS.gene30857:CDS
GCAAAAGAAGAAAAGTACAG+TGG 0.667428 1.1:+49733928 MS.gene30857:CDS
AAAGATCTAGTTGAACCATG+TGG 0.741520 1.1:-49734037 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATACATTTTTTAAATGT+GGG - chr1.1:49734655-49734674 None:intergenic 10.0%
!!! TTAAATACATTTTTTAAATG+TGG - chr1.1:49734656-49734675 None:intergenic 10.0%
!!! AAATACATTTTTTAAATGTG+GGG - chr1.1:49734654-49734673 None:intergenic 15.0%
!!! TTTAATCTTGTTGATTTTAT+GGG + chr1.1:49734830-49734849 MS.gene30857:intron 15.0%
!!! TTTTAATCTTGTTGATTTTA+TGG + chr1.1:49734829-49734848 MS.gene30857:intron 15.0%
!! AAAATTCATAAAACTAGTCA+TGG - chr1.1:49734507-49734526 None:intergenic 20.0%
!! AGTGAAAGATTATTTAAAGA+TGG - chr1.1:49734742-49734761 None:intergenic 20.0%
!! TAAATGCAGAAAATTAAGAT+TGG - chr1.1:49734394-49734413 None:intergenic 20.0%
!!! TTATTTTTTGCCACTTATTT+AGG + chr1.1:49734363-49734382 MS.gene30857:intron 20.0%
!!! TTCTTGAATCATTGATAATA+GGG - chr1.1:49733778-49733797 None:intergenic 20.0%
! ACATTCTCTCAAATTATTAC+CGG + chr1.1:49734290-49734309 MS.gene30857:intron 25.0%
! ATTGGAAAATCCTAAATAAG+TGG - chr1.1:49734376-49734395 None:intergenic 25.0%
! ATTTGAGTAAATATTTGAGC+AGG + chr1.1:49734539-49734558 MS.gene30857:intron 25.0%
! TCTCATATTTGAATTCCATT+CGG - chr1.1:49734809-49734828 None:intergenic 25.0%
!! CTTCTTGAATCATTGATAAT+AGG - chr1.1:49733779-49733798 None:intergenic 25.0%
!! TTAGATGAATTTGGTTTCTT+TGG - chr1.1:49734991-49735010 None:intergenic 25.0%
!!! AACTAAGGTTGATGATAAAA+AGG + chr1.1:49734117-49734136 MS.gene30857:CDS 25.0%
ATTGGACAGAACATGAAAAA+TGG + chr1.1:49733890-49733909 MS.gene30857:CDS 30.0%
GTGGCAAAAAATAAAACCTA+TGG - chr1.1:49734357-49734376 None:intergenic 30.0%
TCTTCAAATACCATTCAACT+TGG + chr1.1:49733728-49733747 MS.gene30857:CDS 30.0%
TTCGAATTATGATTCTGTCT+TGG + chr1.1:49734090-49734109 MS.gene30857:CDS 30.0%
! AATCATTGATAATAGGGTTG+TGG - chr1.1:49733772-49733791 None:intergenic 30.0%
! CAATTGATTCCATTCTGAAA+AGG + chr1.1:49733981-49734000 MS.gene30857:CDS 30.0%
! CTTTTCAGAATGGAATCAAT+TGG - chr1.1:49733983-49734002 None:intergenic 30.0%
! GATAAAAAGGATCAAGTTGT+TGG + chr1.1:49734130-49734149 MS.gene30857:CDS 30.0%
! TTTTTCATGTTCTGTCCAAT+TGG - chr1.1:49733890-49733909 None:intergenic 30.0%
!! AAACACATCTCTAAAATGCA+AGG + chr1.1:49734615-49734634 MS.gene30857:CDS 30.0%
AAAGAAGGTGACAATAGTTG+GGG + chr1.1:49734887-49734906 MS.gene30857:CDS 35.0%
AAAGATCTAGTTGAACCATG+TGG - chr1.1:49734040-49734059 None:intergenic 35.0%
AACAGCAATATGTCTTAGCA+TGG + chr1.1:49733688-49733707 MS.gene30857:CDS 35.0%
ACGAGAAAAGAAAACTAACC+TGG - chr1.1:49734198-49734217 None:intergenic 35.0%
AGCAAAATCTATGTTGAAGG+AGG - chr1.1:49734865-49734884 None:intergenic 35.0%
ATGCTTGCCAATACACTAAA+TGG - chr1.1:49734921-49734940 None:intergenic 35.0%
CAAAGAAGGTGACAATAGTT+GGG + chr1.1:49734886-49734905 MS.gene30857:CDS 35.0%
GAATGTGAACTAAGCAAGTT+TGG + chr1.1:49734460-49734479 MS.gene30857:intron 35.0%
GACACTTTAAATTGGAAGGT+GGG + chr1.1:49734224-49734243 MS.gene30857:intron 35.0%
GGAGTAATTGAGTCAGAATT+TGG - chr1.1:49734019-49734038 None:intergenic 35.0%
GTAACACCGACACTTTAAAT+TGG + chr1.1:49734216-49734235 MS.gene30857:intron 35.0%
TATGTCACACATTTACCGAA+TGG + chr1.1:49734791-49734810 MS.gene30857:intron 35.0%
TCAAATACCATTCAACTTGG+AGG + chr1.1:49733731-49733750 MS.gene30857:CDS 35.0%
TGCAGCAAAATCTATGTTGA+AGG - chr1.1:49734868-49734887 None:intergenic 35.0%
TGTGACTCCATTTAGTGTAT+TGG + chr1.1:49734911-49734930 MS.gene30857:CDS 35.0%
TTCTGTCTTGGCATTAACTA+AGG + chr1.1:49734102-49734121 MS.gene30857:CDS 35.0%
! AACCAATCTCCTTTTCAGAA+TGG - chr1.1:49733993-49734012 None:intergenic 35.0%
! AAGAAGTGGTTTTCTTCTTG+GGG - chr1.1:49733856-49733875 None:intergenic 35.0%
! AGAAGAAGTGGTTTTCTTCT+TGG - chr1.1:49733858-49733877 None:intergenic 35.0%
! AGGTGAAGAATGCACAATTT+TGG + chr1.1:49734946-49734965 MS.gene30857:CDS 35.0%
! AGTTTGGAAGCATATGTTTG+TGG + chr1.1:49734476-49734495 MS.gene30857:intron 35.0%
! CACCTTCCAATTTAAAGTGT+CGG - chr1.1:49734225-49734244 None:intergenic 35.0%
! CATAGATTTTGCTGCAAAGA+AGG + chr1.1:49734872-49734891 MS.gene30857:CDS 35.0%
! CTGCATTTATTGCTTGCTAA+GGG + chr1.1:49734405-49734424 MS.gene30857:intron 35.0%
! GAAGAAGTGGTTTTCTTCTT+GGG - chr1.1:49733857-49733876 None:intergenic 35.0%
! GCAAAAGAAGAAAAGTACAG+TGG + chr1.1:49733928-49733947 MS.gene30857:CDS 35.0%
! TCTGCATTTATTGCTTGCTA+AGG + chr1.1:49734404-49734423 MS.gene30857:intron 35.0%
! TGTATTGGCAAGCATATCAA+AGG + chr1.1:49734926-49734945 MS.gene30857:CDS 35.0%
! TTCCATTCTGAAAAGGAGAT+TGG + chr1.1:49733988-49734007 MS.gene30857:CDS 35.0%
!! CATCTCTAAAATGCAAGGTA+TGG + chr1.1:49734620-49734639 MS.gene30857:intron 35.0%
!!! ATCTTGTTGATTTTATGGGC+AGG + chr1.1:49734834-49734853 MS.gene30857:intron 35.0%
AGGGTTTCTTTGCATTGCAA+AGG + chr1.1:49734573-49734592 MS.gene30857:CDS 40.0%
ATCCAACCCTGCATCTAAAT+CGG + chr1.1:49734162-49734181 MS.gene30857:CDS 40.0%
CACCGACACTTTAAATTGGA+AGG + chr1.1:49734220-49734239 MS.gene30857:intron 40.0%
CGACACTTTAAATTGGAAGG+TGG + chr1.1:49734223-49734242 MS.gene30857:intron 40.0%
GCAAAGAAGGTGACAATAGT+TGG + chr1.1:49734885-49734904 MS.gene30857:CDS 40.0%
GGGTTTCTTTGCATTGCAAA+GGG + chr1.1:49734574-49734593 MS.gene30857:CDS 40.0%
TCTGACTCAATTACTCCACA+TGG + chr1.1:49734022-49734041 MS.gene30857:CDS 40.0%
TCTTCTTCTTCCAAGCCAAT+TGG + chr1.1:49733872-49733891 MS.gene30857:CDS 40.0%
TGGCTTAGAAGAGTAACTGT+TGG - chr1.1:49733963-49733982 None:intergenic 40.0%
TTGAGCAGGTTGTAGTATTG+AGG + chr1.1:49734553-49734572 MS.gene30857:intron 40.0%
TTGGACACATTCAACACATG+TGG + chr1.1:49734265-49734284 MS.gene30857:intron 40.0%
! AAGAAAACTAACCTGGCTTG+AGG - chr1.1:49734191-49734210 None:intergenic 40.0%
! GCTAAGACATATTGCTGTTG+AGG - chr1.1:49733687-49733706 None:intergenic 40.0%
! GTTTTCTTCTTGGGGTCAAT+AGG - chr1.1:49733848-49733867 None:intergenic 40.0%
! TGAGCAGGTTGTAGTATTGA+GGG + chr1.1:49734554-49734573 MS.gene30857:intron 40.0%
ACAAGGAGCAGTGATAGTCT+TGG - chr1.1:49733810-49733829 None:intergenic 45.0%
AGGAACCCGATTTAGATGCA+GGG - chr1.1:49734171-49734190 None:intergenic 45.0%
CATGTTCTGTCCAATTGGCT+TGG - chr1.1:49733885-49733904 None:intergenic 45.0%
GGTAGCTGAGAAGAAGAACA+AGG - chr1.1:49733827-49733846 None:intergenic 45.0%
GTCTAGTGTCAATGCTCCAT+AGG + chr1.1:49734338-49734357 MS.gene30857:intron 45.0%
TCCAACCCTGCATCTAAATC+GGG + chr1.1:49734163-49734182 MS.gene30857:CDS 45.0%
TGAATGTGTCCAACCTGTGT+TGG - chr1.1:49734258-49734277 None:intergenic 45.0%
! AGTGACGCAGCTTTTGTAGA+TGG + chr1.1:49734064-49734083 MS.gene30857:CDS 45.0%
! GACTTTGCCTCCAAGTTGAA+TGG - chr1.1:49733741-49733760 None:intergenic 45.0%
! TGCATTGCAAAGGGTGTGAA+GGG + chr1.1:49734583-49734602 MS.gene30857:CDS 45.0%
! TGGCTTGGAAGAAGAAGAAG+TGG - chr1.1:49733870-49733889 None:intergenic 45.0%
! TTACCGGTGTCAATGAGAAG+TGG + chr1.1:49734306-49734325 MS.gene30857:intron 45.0%
! TTGCATTGCAAAGGGTGTGA+AGG + chr1.1:49734582-49734601 MS.gene30857:CDS 45.0%
! TTGCCACTTCTCATTGACAC+CGG - chr1.1:49734312-49734331 None:intergenic 45.0%
ACCCGATTTAGATGCAGGGT+TGG - chr1.1:49734167-49734186 None:intergenic 50.0%
GAGGAACCCGATTTAGATGC+AGG - chr1.1:49734172-49734191 None:intergenic 50.0%
TAAATCGGGTTCCTCAAGCC+AGG + chr1.1:49734177-49734196 MS.gene30857:CDS 50.0%
!! GCACAATTTTGGCCAGCACT+TGG + chr1.1:49734957-49734976 MS.gene30857:CDS 50.0%
!! TTGGTGTCAGATCCAAGTGC+TGG - chr1.1:49734972-49734991 None:intergenic 50.0%
GTCCAACCTGTGTTGGACAC+AGG - chr1.1:49734251-49734270 None:intergenic 55.0%
GTCCTGTGTCCAACACAGGT+TGG + chr1.1:49734246-49734265 MS.gene30857:intron 55.0%
! AAGGGTGTGAAGGGAAGCTG+AGG + chr1.1:49734592-49734611 MS.gene30857:CDS 55.0%
GTGGGTCCTGTGTCCAACAC+AGG + chr1.1:49734242-49734261 MS.gene30857:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 49733650 49735010 49733650 ID=MS.gene30857
chr1.1 mRNA 49733650 49735010 49733650 ID=MS.gene30857.t1;Parent=MS.gene30857
chr1.1 exon 49733650 49734198 49733650 ID=MS.gene30857.t1.exon1;Parent=MS.gene30857.t1
chr1.1 CDS 49733650 49734198 49733650 ID=cds.MS.gene30857.t1;Parent=MS.gene30857.t1
chr1.1 exon 49734561 49734636 49734561 ID=MS.gene30857.t1.exon2;Parent=MS.gene30857.t1
chr1.1 CDS 49734561 49734636 49734561 ID=cds.MS.gene30857.t1;Parent=MS.gene30857.t1
chr1.1 exon 49734856 49735010 49734856 ID=MS.gene30857.t1.exon3;Parent=MS.gene30857.t1
chr1.1 CDS 49734856 49735010 49734856 ID=cds.MS.gene30857.t1;Parent=MS.gene30857.t1
Gene Sequence

>MS.gene30857

ATGAAAAGAATGGATATCTTCTGTGCTTCACAAGCCTCAACAGCAATATGTCTTAGCATGGAACAAGCTTCATGCTCATCTTCAAATACCATTCAACTTGGAGGCAAAGTCATTGATCGCCACAACCCTATTATCAATGATTCAAGAAGAAGCACTTCCAAGACTATCACTGCTCCTTGTTCTTCTTCTCAGCTACCTATTGACCCCAAGAAGAAAACCACTTCTTCTTCTTCCAAGCCAATTGGACAGAACATGAAAAATGGAGCTAAAGTTTATGAGCAAAAGAAGAAAAGTACAGTGGAAAATGTCACCAACAGTTACTCTTCTAAGCCAATTGATTCCATTCTGAAAAGGAGATTGGTTAAGCCAAATTCTGACTCAATTACTCCACATGGTTCAACTAGATCTTTGCTCAGTGACGCAGCTTTTGTAGATGGCTCTTCGAATTATGATTCTGTCTTGGCATTAACTAAGGTTGATGATAAAAAGGATCAAGTTGTTGGTCAAAATGAATCCAACCCTGCATCTAAATCGGGTTCCTCAAGCCAGGTTAGTTTTCTTTTCTCGTAACACCGACACTTTAAATTGGAAGGTGGGTCCTGTGTCCAACACAGGTTGGACACATTCAACACATGTGGTTACATTCTCTCAAATTATTACCGGTGTCAATGAGAAGTGGCAATGTCGTGTCTAGTGTCAATGCTCCATAGGTTTTATTTTTTGCCACTTATTTAGGATTTTCCAATCTTAATTTTCTGCATTTATTGCTTGCTAAGGGCTCTCTAATTGTAAATTTATGATATGCTTCGTGAATGTGAACTAAGCAAGTTTGGAAGCATATGTTTGTGGAATAACCATGACTAGTTTTATGAATTTTCACTAATTGATGATTTGAGTAAATATTTGAGCAGGTTGTAGTATTGAGGGTTTCTTTGCATTGCAAAGGGTGTGAAGGGAAGCTGAGGAAACACATCTCTAAAATGCAAGGTATGGACTTGATTCCCCACATTTAAAAAATGTATTTAAACGCGCTAATTGATCTCGATCATCAATTTGAGATCAATCAATTCACGTGAAATCTTCACGAAACCATCTTTAAATAATCTTTCACTGTACAATTTAAAATCGAAAAGTCAATAAATATGTCACACATTTACCGAATGGAATTCAAATATGAGATTTTAATCTTGTTGATTTTATGGGCAGGAGTTACCTCCTTCAACATAGATTTTGCTGCAAAGAAGGTGACAATAGTTGGGGATGTGACTCCATTTAGTGTATTGGCAAGCATATCAAAGGTGAAGAATGCACAATTTTGGCCAGCACTTGGATCTGACACCAAAGAAACCAAATTCATCTAA
Protein sequence

>MS.gene30857.t1

MKRMDIFCASQASTAICLSMEQASCSSSNTIQLGGKVIDRHNPIINDSRRSTSKTITAPCSSSQLPIDPKKKTTSSSSKPIGQNMKNGAKVYEQKKKSTVENVTNSYSSKPIDSILKRRLVKPNSDSITPHGSTRSLLSDAAFVDGSSNYDSVLALTKVDDKKDQVVGQNESNPASKSGSSSQVVVLRVSLHCKGCEGKLRKHISKMQGVTSFNIDFAAKKVTIVGDVTPFSVLASISKVKNAQFWPALGSDTKETKFI