AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068582


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAACAACAACCTATTAA+TGG 0.266540 1.2:-41835698 MS.gene068582:CDS
AGCTAAGGACTACCATTAAT+AGG 0.397702 1.2:+41835686 None:intergenic
ACAACATCTTCAATCAACTA+TGG 0.410424 1.2:-41835894 MS.gene068582:CDS
TGAGAGTAAGAAGAAGTTGT+TGG 0.413123 1.2:+41835716 None:intergenic
TTTCCTACTTCACAAGCCTT+AGG 0.446992 1.2:-41835962 MS.gene068582:CDS
GTGTTCCATGTTGGATAAGA+AGG 0.460404 1.2:+41835764 None:intergenic
CCACCTAAGGCTTGTGAAGT+AGG 0.467927 1.2:+41835959 None:intergenic
AAAGAGGATGCTGTTGTTGA+TGG 0.471809 1.2:+41835828 None:intergenic
GCTGTTGTTGATGGTGATGA+TGG 0.514460 1.2:+41835837 None:intergenic
CATGGAACACAAACTCAACT+AGG 0.515152 1.2:-41835751 MS.gene068582:CDS
GTTGAGTTTGTGTTCCATGT+TGG 0.535384 1.2:+41835755 None:intergenic
CCTACTTCACAAGCCTTAGG+TGG 0.566151 1.2:-41835959 MS.gene068582:CDS
AGTAAGAAGAAGTTGTTGGA+TGG 0.569777 1.2:+41835720 None:intergenic
CATCACCTTCTTATCCAACA+TGG 0.575046 1.2:-41835769 MS.gene068582:CDS
TGGTGATGATGGTAGAGATG+TGG 0.592964 1.2:+41835848 None:intergenic
GGTGATGATGATAATGATGA+TGG 0.595521 1.2:+41835785 None:intergenic
GATGATGTGAATCTATAAAG+AGG 0.609056 1.2:+41835812 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAACATCTTCAATCAACTA+TGG - chr1.2:41835825-41835844 MS.gene068582:CDS 30.0%
GATGATGTGAATCTATAAAG+AGG + chr1.2:41835910-41835929 None:intergenic 30.0%
TCTCAACAACAACCTATTAA+TGG - chr1.2:41836021-41836040 MS.gene068582:CDS 30.0%
AGTAAGAAGAAGTTGTTGGA+TGG + chr1.2:41836002-41836021 None:intergenic 35.0%
TGAGAGTAAGAAGAAGTTGT+TGG + chr1.2:41836006-41836025 None:intergenic 35.0%
! GGTGATGATGATAATGATGA+TGG + chr1.2:41835937-41835956 None:intergenic 35.0%
AAAGAGGATGCTGTTGTTGA+TGG + chr1.2:41835894-41835913 None:intergenic 40.0%
CATCACCTTCTTATCCAACA+TGG - chr1.2:41835950-41835969 MS.gene068582:CDS 40.0%
CATGGAACACAAACTCAACT+AGG - chr1.2:41835968-41835987 MS.gene068582:CDS 40.0%
GTGTTCCATGTTGGATAAGA+AGG + chr1.2:41835958-41835977 None:intergenic 40.0%
GTTGAGTTTGTGTTCCATGT+TGG + chr1.2:41835967-41835986 None:intergenic 40.0%
TTTCCTACTTCACAAGCCTT+AGG - chr1.2:41835757-41835776 MS.gene068582:CDS 40.0%
!! GCTGTTGTTGATGGTGATGA+TGG + chr1.2:41835885-41835904 None:intergenic 45.0%
!! TGCGTTTTGATGACCACCTA+AGG + chr1.2:41835776-41835795 None:intergenic 45.0%
!! TGGTGATGATGGTAGAGATG+TGG + chr1.2:41835874-41835893 None:intergenic 45.0%
CCACCTAAGGCTTGTGAAGT+AGG + chr1.2:41835763-41835782 None:intergenic 50.0%
CCTACTTCACAAGCCTTAGG+TGG - chr1.2:41835760-41835779 MS.gene068582:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 41835696 41836045 41835696 ID=MS.gene068582
chr1.2 mRNA 41835696 41836045 41835696 ID=MS.gene068582.t1;Parent=MS.gene068582
chr1.2 exon 41835696 41836045 41835696 ID=MS.gene068582.t1.exon1;Parent=MS.gene068582.t1
chr1.2 CDS 41835696 41836045 41835696 ID=cds.MS.gene068582.t1;Parent=MS.gene068582.t1
Gene Sequence

>MS.gene068582

AGACAACAACAATAACAACGAAAACAACAGAAGATTTGAATGTCATTACTGTTGTAGAAACTTTCCTACTTCACAAGCCTTAGGTGGTCATCAAAACGCACACAAAAGAGAACGTCAACACGCAAAACGACAACATCTTCAATCAACTATGGTACATGCTACTACTTTCTCTGATCCACATCTCTACCATCATCACCATCAACAACAGCATCCTCTTTATAGATTCACATCATCATCACCATCATCATTATCATCATCACCTTCTTATCCAACATGGAACACAAACTCAACTAGGTTCTATAACCATCCAACAACTTCTTCTTACTCTCAACAACAACCTATTAATGGTA

Protein sequence

>MS.gene068582.t1

RQQQUQRKQQKIUMSLLLUKLSYFTSLRWSSKRTQKRTSTRKTTTSSINYGTCYYFLUSTSLPSSPSTTASSLUIHIIITIIIIIITFLSNMEHKLNUVLUPSNNFFLLSTTTYUW