AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056191


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056191.t1 MTR_8g037933 91.892 185 3 2 1 179 1 179 1.04e-109 310
MS.gene056191.t1 MTR_0695s0010 88.889 36 4 0 35 70 18 53 7.40e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCATTATGTCCTTCT+AGG 0.088496 8.1:-65623418 None:intergenic
TTTGGAATTGGCTTGTAAAT+TGG 0.156604 8.1:-65623707 None:intergenic
CTTTGGAATAGGCTTGTAAA+TGG 0.212935 8.1:-65623612 None:intergenic
TTTGGAATAGGCTTGTAAAT+TGG 0.225594 8.1:-65623659 None:intergenic
TTTGGAATAGGCTTGTAAAT+TGG 0.233261 8.1:-65623737 None:intergenic
GGAATTGGTTTCTTGAATAT+TGG 0.237384 8.1:-65623500 None:intergenic
TTATAGATTGGAACGTATTT+TGG 0.239696 8.1:-65623569 None:intergenic
TTGTGAATAGGTTTATAAAA+TGG 0.240012 8.1:-65623521 None:intergenic
ATTGGAACAGGCTTGTAAAT+TGG 0.276520 8.1:-65623641 None:intergenic
ATTGGAACAGGCTTGTAAAT+TGG 0.276520 8.1:-65623689 None:intergenic
TTTGGTATAGGTTTGTAAAT+TGG 0.283056 8.1:-65623551 None:intergenic
ATTGGAATTACCTTTGGAAT+TGG 0.313795 8.1:-65623719 None:intergenic
ATTGGAATTACCTTTGGAAT+AGG 0.316743 8.1:-65623623 None:intergenic
ATTGGAATGTCTTTGTGAAT+AGG 0.322130 8.1:-65623533 None:intergenic
CAAAAGTGGCCTTTGCAAAT+TGG 0.325296 8.1:-65622499 None:intergenic
CAATTTGCAAAGGCCACTTT+TGG 0.327118 8.1:+65622500 MS.gene056191:CDS
ATTGGAATAACCTTTGGAAT+AGG 0.333599 8.1:-65623671 None:intergenic
ATGGGAACTACCTTTGGAAT+AGG 0.351240 8.1:-65623749 None:intergenic
ATAGGCTTGTAAATGGGAAT+TGG 0.370906 8.1:-65623605 None:intergenic
TTTACAATGGGAACTACCTT+TGG 0.372794 8.1:-65623755 None:intergenic
GTAGCAAGAAATGATCCTTC+AGG 0.392442 8.1:+65622576 MS.gene056191:CDS
ATAGGTTTATAAAATGGAAT+TGG 0.398934 8.1:-65623515 None:intergenic
TTGTAAATTGGAATAACCTT+TGG 0.409850 8.1:-65623677 None:intergenic
TTGTAAATTGGAATTACCTT+TGG 0.416670 8.1:-65623629 None:intergenic
TTGTAAATTGGAATTACCTT+TGG 0.416670 8.1:-65623725 None:intergenic
TAATGGATTGGCTTTACAAT+GGG 0.428101 8.1:-65623767 None:intergenic
ATTGGCTTGTAAATTGGAAC+AGG 0.429206 8.1:-65623701 None:intergenic
ATAGGCTTGTAAATTGGAAC+AGG 0.440807 8.1:-65623653 None:intergenic
ATGGGAATTGGTTTGTGGAT+AGG 0.448083 8.1:-65623593 None:intergenic
GCTTCCCATCTGTGTTCACT+TGG 0.449323 8.1:-65623367 None:intergenic
GCATGATAATCTTAATGGAT+TGG 0.454921 8.1:-65623779 None:intergenic
TTGTGGATAGGCTTATAGAT+TGG 0.461177 8.1:-65623581 None:intergenic
GCAACAAAGACAAACCTAGA+AGG 0.464842 8.1:+65623404 MS.gene056191:CDS
TGTAAATGGGAATTGGTTTG+TGG 0.491139 8.1:-65623598 None:intergenic
TTTGGAATAGGCTTGTAAAT+GGG 0.493642 8.1:-65623611 None:intergenic
AACAGAAGTAGCATAACAAA+AGG 0.510420 8.1:-65622553 None:intergenic
TTAATGGATTGGCTTTACAA+TGG 0.537191 8.1:-65623768 None:intergenic
GGAAACCAAGTGAACACAGA+TGG 0.546430 8.1:+65623362 MS.gene056191:intron
CATCTAAAACACAAACTCAA+AGG 0.559830 8.1:+65623461 MS.gene056191:CDS
CATTTACAAGCCTATTCCAA+AGG 0.578514 8.1:+65623613 MS.gene056191:CDS
GAAACCAAGTGAACACAGAT+GGG 0.582541 8.1:+65623363 MS.gene056191:CDS
GGAACCCAGAAGGCCAAAAG+TGG 0.583318 8.1:-65622513 None:intergenic
AATTTACAAGCCTATTCCAA+AGG 0.591786 8.1:+65623661 MS.gene056191:CDS
AATTTACAAGCCAATTCCAA+AGG 0.595922 8.1:+65623709 MS.gene056191:CDS
GAAAAGCTTCCAATTTGCAA+AGG 0.597703 8.1:+65622490 MS.gene056191:CDS
AAGGTGACAAGGAGCAACAA+AGG 0.600910 8.1:-65622534 None:intergenic
TGGGAAGCTTGCAAATGAAG+AGG 0.610090 8.1:+65623382 MS.gene056191:CDS
GAGCAACAAAGGAACCCAGA+AGG 0.618628 8.1:-65622523 None:intergenic
AATTTACAAGCCTATTCCAA+AGG 0.618821 8.1:+65623739 MS.gene056191:CDS
TAGCATAACAAAAGGTGACA+AGG 0.637763 8.1:-65622545 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTGAGTTTTTAATAAA+AGG - chr8.1:65623036-65623055 None:intergenic 10.0%
!!! TTGAATAAAATTTTATTTGA+AGG + chr8.1:65622986-65623005 MS.gene056191:intron 10.0%
!! AAAAAAAAAAAACACAAACT+TGG + chr8.1:65623283-65623302 MS.gene056191:intron 15.0%
!! TACATATATTGTACATTTAT+AGG - chr8.1:65622625-65622644 None:intergenic 15.0%
!! AATGAAGAAGAAAAATTCAA+AGG + chr8.1:65623431-65623450 MS.gene056191:CDS 20.0%
!! ACATTTATATTTCCCTTATT+TGG + chr8.1:65622711-65622730 MS.gene056191:intron 20.0%
!! ATAGGTTTATAAAATGGAAT+TGG - chr8.1:65623518-65623537 None:intergenic 20.0%
!! TTGTGAATAGGTTTATAAAA+TGG - chr8.1:65623524-65623543 None:intergenic 20.0%
! AGTAGTAACTATTCATAGAT+TGG + chr8.1:65622660-65622679 MS.gene056191:intron 25.0%
! GTAGTAACTATTCATAGATT+GGG + chr8.1:65622661-65622680 MS.gene056191:intron 25.0%
! TTACTCAAGTTATAAACTCT+AGG - chr8.1:65623324-65623343 None:intergenic 25.0%
! TTGTAAATTGGAATAACCTT+TGG - chr8.1:65623680-65623699 None:intergenic 25.0%
! TTGTAAATTGGAATTACCTT+TGG - chr8.1:65623632-65623651 None:intergenic 25.0%
! TTTGGTATAGGTTTGTAAAT+TGG - chr8.1:65623554-65623573 None:intergenic 25.0%
!! TTATAGATTGGAACGTATTT+TGG - chr8.1:65623572-65623591 None:intergenic 25.0%
!! TTTTATGACTAATCTCTGTT+AGG - chr8.1:65623224-65623243 None:intergenic 25.0%
!!! CTCTCTTTTTTACCAAATAA+GGG - chr8.1:65622726-65622745 None:intergenic 25.0%
!!! GTTTCTTGAATATTGGTTTT+TGG - chr8.1:65623496-65623515 None:intergenic 25.0%
!!! TCTCTCTTTTTTACCAAATA+AGG - chr8.1:65622727-65622746 None:intergenic 25.0%
AACAGAAGTAGCATAACAAA+AGG - chr8.1:65622556-65622575 None:intergenic 30.0%
AAGAGAGAGAGAGAAATTTA+TGG + chr8.1:65622739-65622758 MS.gene056191:intron 30.0%
AATTTACAAGCCAATTCCAA+AGG + chr8.1:65623709-65623728 MS.gene056191:CDS 30.0%
AATTTACAAGCCTATTCCAA+AGG + chr8.1:65623661-65623680 MS.gene056191:CDS 30.0%
ACCATTTAAACATGACTTTG+AGG - chr8.1:65622839-65622858 None:intergenic 30.0%
ATTGGAATGTCTTTGTGAAT+AGG - chr8.1:65623536-65623555 None:intergenic 30.0%
GCTTGAAAATATCATTGTTC+AGG + chr8.1:65622773-65622792 MS.gene056191:intron 30.0%
TCCTCAAAGTCATGTTTAAA+TGG + chr8.1:65622835-65622854 MS.gene056191:intron 30.0%
TTAGGAAATTTGACTGATCA+TGG - chr8.1:65623206-65623225 None:intergenic 30.0%
! CATCTAAAACACAAACTCAA+AGG + chr8.1:65623461-65623480 MS.gene056191:CDS 30.0%
! GGAATTGGTTTCTTGAATAT+TGG - chr8.1:65623503-65623522 None:intergenic 30.0%
! TAATTTTGCATTTCTTGTGC+AGG + chr8.1:65623341-65623360 MS.gene056191:intron 30.0%
! TTTGGAATAGGCTTGTAAAT+GGG - chr8.1:65623614-65623633 None:intergenic 30.0%
! TTTGGAATAGGCTTGTAAAT+TGG - chr8.1:65623614-65623633 None:intergenic 30.0%
!! ATTGGAATAACCTTTGGAAT+AGG - chr8.1:65623674-65623693 None:intergenic 30.0%
!! ATTGGAATTACCTTTGGAAT+AGG - chr8.1:65623626-65623645 None:intergenic 30.0%
!! ATTGGAATTACCTTTGGAAT+TGG - chr8.1:65623626-65623645 None:intergenic 30.0%
!! GTTTTTGTTTACTCTTGGTA+TGG + chr8.1:65622687-65622706 MS.gene056191:intron 30.0%
!! TAATGGATTGGCTTTACAAT+GGG - chr8.1:65623770-65623789 None:intergenic 30.0%
!! TTAATGGATTGGCTTTACAA+TGG - chr8.1:65623771-65623790 None:intergenic 30.0%
!! TTTGGAATTGGCTTGTAAAT+TGG - chr8.1:65623710-65623729 None:intergenic 30.0%
!!! ATTGGAACGTATTTTGGTAT+AGG - chr8.1:65623566-65623585 None:intergenic 30.0%
!!! GGTTAGTTTTTGTTTACTCT+TGG + chr8.1:65622682-65622701 MS.gene056191:intron 30.0%
AAAATGACTCACAAACCTGA+AGG - chr8.1:65622594-65622613 None:intergenic 35.0%
ACTATCATCATCATCACACA+AGG - chr8.1:65622883-65622902 None:intergenic 35.0%
ATAGGCTTGTAAATGGGAAT+TGG - chr8.1:65623608-65623627 None:intergenic 35.0%
ATAGGCTTGTAAATTGGAAC+AGG - chr8.1:65623656-65623675 None:intergenic 35.0%
ATTGGAACAGGCTTGTAAAT+TGG - chr8.1:65623644-65623663 None:intergenic 35.0%
CATTTACAAGCCTATTCCAA+AGG + chr8.1:65623613-65623632 MS.gene056191:CDS 35.0%
CTTCTTCATTATGTCCTTCT+AGG - chr8.1:65623421-65623440 None:intergenic 35.0%
GAAAAGCTTCCAATTTGCAA+AGG + chr8.1:65622490-65622509 MS.gene056191:CDS 35.0%
TAGCATAACAAAAGGTGACA+AGG - chr8.1:65622548-65622567 None:intergenic 35.0%
TTGTGGATAGGCTTATAGAT+TGG - chr8.1:65623584-65623603 None:intergenic 35.0%
TTTACAATGGGAACTACCTT+TGG - chr8.1:65623758-65623777 None:intergenic 35.0%
!! ATTGGCTTGTAAATTGGAAC+AGG - chr8.1:65623704-65623723 None:intergenic 35.0%
!! CTTTGGAATAGGCTTGTAAA+TGG - chr8.1:65623615-65623634 None:intergenic 35.0%
!! TGTAAATGGGAATTGGTTTG+TGG - chr8.1:65623601-65623620 None:intergenic 35.0%
CAAAAGTGGCCTTTGCAAAT+TGG - chr8.1:65622502-65622521 None:intergenic 40.0%
GAAACCAAGTGAACACAGAT+GGG + chr8.1:65623363-65623382 MS.gene056191:CDS 40.0%
GCAACAAAGACAAACCTAGA+AGG + chr8.1:65623404-65623423 MS.gene056191:CDS 40.0%
GTAGCAAGAAATGATCCTTC+AGG + chr8.1:65622576-65622595 MS.gene056191:CDS 40.0%
TACTTGAACCAATGTAGTGC+CGG - chr8.1:65623113-65623132 None:intergenic 40.0%
TCAAACCAATACATGCACAC+CGG + chr8.1:65623091-65623110 MS.gene056191:intron 40.0%
!! ATGGGAACTACCTTTGGAAT+AGG - chr8.1:65623752-65623771 None:intergenic 40.0%
!! ATGGGAATTGGTTTGTGGAT+AGG - chr8.1:65623596-65623615 None:intergenic 40.0%
!! CAATTTGCAAAGGCCACTTT+TGG + chr8.1:65622500-65622519 MS.gene056191:CDS 40.0%
AAGGTGACAAGGAGCAACAA+AGG - chr8.1:65622537-65622556 None:intergenic 45.0%
ACAAACTTGGCCATTGAGCT+AGG + chr8.1:65623296-65623315 MS.gene056191:intron 45.0%
GGAAACCAAGTGAACACAGA+TGG + chr8.1:65623362-65623381 MS.gene056191:intron 45.0%
TGGGAAGCTTGCAAATGAAG+AGG + chr8.1:65623382-65623401 MS.gene056191:CDS 45.0%
ACTCTAGGTGCCTAGCTCAA+TGG - chr8.1:65623309-65623328 None:intergenic 50.0%
GAGCAACAAAGGAACCCAGA+AGG - chr8.1:65622526-65622545 None:intergenic 50.0%
GCTTCCCATCTGTGTTCACT+TGG - chr8.1:65623370-65623389 None:intergenic 50.0%
!! TAGTGCCGGTGTGCATGTAT+TGG - chr8.1:65623099-65623118 None:intergenic 50.0%
!!! AAAGGCCACTTTTGGCCTTC+TGG + chr8.1:65622508-65622527 MS.gene056191:CDS 50.0%
!!! AAGGCCACTTTTGGCCTTCT+GGG + chr8.1:65622509-65622528 MS.gene056191:CDS 50.0%
CATGCACACCGGCACTACAT+TGG + chr8.1:65623102-65623121 MS.gene056191:intron 55.0%
GGAACCCAGAAGGCCAAAAG+TGG - chr8.1:65622516-65622535 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 65622486 65623790 65622486 ID=MS.gene056191
chr8.1 mRNA 65622486 65623790 65622486 ID=MS.gene056191.t1;Parent=MS.gene056191
chr8.1 exon 65622486 65622597 65622486 ID=MS.gene056191.t1.exon1;Parent=MS.gene056191.t1
chr8.1 CDS 65622486 65622597 65622486 ID=cds.MS.gene056191.t1;Parent=MS.gene056191.t1
chr8.1 exon 65623363 65623790 65623363 ID=MS.gene056191.t1.exon2;Parent=MS.gene056191.t1
chr8.1 CDS 65623363 65623790 65623363 ID=cds.MS.gene056191.t1;Parent=MS.gene056191.t1
Gene Sequence

>MS.gene056191

ATGAGAAAAGCTTCCAATTTGCAAAGGCCACTTTTGGCCTTCTGGGTTCCTTTGTTGCTCCTTGTCACCTTTTGTTATGCTACTTCTGTTGTAGCAAGAAATGATCCTTCAGGTTTGTGAGTCATTTTGATATTGACCTATAAATGTACAATATATGTACACAATATATATAGTAGTAGTAACTATTCATAGATTGGGTTAGTTTTTGTTTACTCTTGGTATGGTACATTTATATTTCCCTTATTTGGTAAAAAAGAGAGAGAGAGAAATTTATGGTGTTAAAAATCGCTTGAAAATATCATTGTTCAGGTTGTCACTTGTAGTAGTATGTTTGATGAAGCATTTCAGCTCCTCAAAGTCATGTTTAAATGGTCTTTTTTTATCTTTACTTTTTCCTTGTGTGATGATGATGATAGTAAAGTAAAATAAAATTTAATTTTAAAAGCATATATGTTTAATTAAAAGTGAAAATATATTGTTTTTGTATTGAGCAGAAAATTTTGAATAAAATTTTATTTGAAGGTGATATTTCTTTTATTGTAATTCTCCTTTTATTAAAAACTCAATTATTTGTGAGTTAAGAAACTAATTAATAAGTTTCATATTCAAACCAATACATGCACACCGGCACTACATTGGTTCAAGTAGAATTGAACTCGAATCTCTTAAATAAGTCGAACATTGTGAATGAAAAAAATATGATCGAGAGAGAAAACTCCATGATCAGTCAAATTTCCTAACAGAGATTAGTCATAAAAAAGTTGATAGATATTTCGCATAAATAAGATTGTTACAAAAAAAAAAAAAAACACAAACTTGGCCATTGAGCTAGGCACCTAGAGTTTATAACTTGAGTAATTTTGCATTTCTTGTGCAGGAAACCAAGTGAACACAGATGGGAAGCTTGCAAATGAAGAGGCAACAAAGACAAACCTAGAAGGACATAATGAAGAAGAAAAATTCAAAGGATTTTTTCATCTAAAACACAAACTCAAAGGATATTTTCACCAAAAACCAATATTCAAGAAACCAATTCCATTTTATAAACCTATTCACAAAGACATTCCAATTTACAAACCTATACCAAAATACGTTCCAATCTATAAGCCTATCCACAAACCAATTCCCATTTACAAGCCTATTCCAAAGGTAATTCCAATTTACAAGCCTGTTCCAATTTACAAGCCTATTCCAAAGGTTATTCCAATTTACAAGCCTGTTCCAATTTACAAGCCAATTCCAAAGGTAATTCCAATTTACAAGCCTATTCCAAAGGTAGTTCCCATTGTAAAGCCAATCCATTAA

Protein sequence

>MS.gene056191.t1

MRKASNLQRPLLAFWVPLLLLVTFCYATSVVARNDPSGNQVNTDGKLANEEATKTNLEGHNEEEKFKGFFHLKHKLKGYFHQKPIFKKPIPFYKPIHKDIPIYKPIPKYVPIYKPIHKPIPIYKPIPKVIPIYKPVPIYKPIPKVIPIYKPVPIYKPIPKVIPIYKPIPKVVPIVKPIH