AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068654

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068654.t1 MTR_2g070780 92.962 341 24 0 6 346 1 341 0.0 647
MS.gene068654.t1 MTR_6g034190 58.462 520 192 4 6 510 1 511 0.0 595
MS.gene068654.t1 MTR_2g072240 50.487 513 181 8 6 510 1 448 3.39e-167 480
MS.gene068654.t1 MTR_2g071700 71.598 338 92 2 6 339 1 338 1.44e-160 459
MS.gene068654.t1 MTR_2g071720 77.348 181 25 1 346 510 1 181 1.12e-96 290
MS.gene068654.t1 MTR_2g070820 90.090 111 11 0 280 390 1 111 4.21e-64 204
MS.gene068654.t1 MTR_2g070660 56.853 197 51 3 6 198 1 167 2.20e-60 196
MS.gene068654.t1 MTR_2g070810 97.872 47 1 0 6 52 49 95 5.01e-24 96.7
MS.gene068654.t1 MTR_2g047858 24.330 448 275 15 18 457 111 502 5.86e-16 80.9
MS.gene068654.t1 MTR_1g016340 22.667 450 287 13 17 457 15 412 3.00e-12 68.9
MS.gene068654.t1 MTR_1g016340 22.667 450 287 13 17 457 10 407 3.38e-12 68.9
MS.gene068654.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 4.12e-12 68.9
MS.gene068654.t1 MTR_8g468470 26.096 456 281 21 21 457 13 431 4.63e-12 68.6
MS.gene068654.t1 MTR_7g095350 27.331 311 193 11 27 328 21 307 5.05e-12 68.6
MS.gene068654.t1 MTR_4g024770 40.845 71 42 0 2 72 4 74 5.05e-12 62.0
MS.gene068654.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 5.07e-12 68.2
MS.gene068654.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 5.91e-12 68.2
MS.gene068654.t1 MTR_7g095350 27.331 311 193 11 27 328 21 307 6.16e-12 68.2
MS.gene068654.t1 MTR_8g014180 52.727 55 26 0 6 60 1 55 8.12e-12 60.8
MS.gene068654.t1 MTR_2g082860 56.000 50 22 0 21 70 26 75 1.57e-11 62.0
MS.gene068654.t1 MTR_6g013240 23.762 303 198 10 10 302 60 339 1.76e-11 67.0
MS.gene068654.t1 MTR_7g007370 50.820 61 30 0 6 66 1 61 1.78e-11 65.9
MS.gene068654.t1 MTR_6g013380 24.242 297 192 10 16 302 61 334 2.23e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068654.t1 AT1G80960 27.273 462 253 17 22 457 11 415 4.51e-22 99.4
MS.gene068654.t1 AT1G80960 27.273 462 253 17 22 457 50 454 5.51e-22 99.4
MS.gene068654.t1 AT1G80960 27.273 462 253 17 22 457 50 454 5.51e-22 99.4
MS.gene068654.t1 AT1G80960 27.273 462 253 17 22 457 50 454 5.51e-22 99.4
MS.gene068654.t1 AT1G80960 31.023 303 151 11 22 308 50 310 2.08e-21 96.7
MS.gene068654.t1 AT1G80960 31.023 303 151 11 22 308 50 310 2.26e-21 97.4
MS.gene068654.t1 AT1G67390 26.667 300 182 9 7 302 27 292 2.04e-16 82.4
MS.gene068654.t1 AT3G59190 24.624 532 297 27 15 488 5 490 8.26e-16 80.5
MS.gene068654.t1 AT3G26922 27.241 290 159 8 21 302 13 258 6.30e-15 76.3
MS.gene068654.t1 AT3G26922 27.241 290 159 8 21 302 13 258 6.30e-15 76.3
MS.gene068654.t1 AT1G78760 22.863 503 284 18 11 481 5 435 4.63e-13 71.6
MS.gene068654.t1 AT5G41830 26.667 240 138 7 11 243 2 210 5.82e-13 71.6
MS.gene068654.t1 AT5G62970 22.845 464 266 17 29 457 10 416 6.25e-13 71.2
MS.gene068654.t1 AT3G59190 26.176 340 188 16 15 337 5 298 1.33e-12 69.7
MS.gene068654.t1 AT5G22730 22.016 486 276 18 9 457 15 434 1.27e-11 67.0
MS.gene068654.t1 AT5G22730 22.016 486 276 18 9 457 15 434 1.27e-11 67.0
MS.gene068654.t1 AT3G60790 27.124 306 177 14 7 306 40 305 1.48e-11 67.0
MS.gene068654.t1 AT3G49030 24.835 455 258 17 20 457 19 406 1.57e-11 67.0
MS.gene068654.t1 AT3G52680 53.731 67 30 1 1 67 1 66 5.91e-11 64.7
MS.gene068654.t1 AT5G41840 25.769 260 146 9 22 266 14 241 8.72e-11 64.7

Find 101 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAATCTGTTGAGGATTA+AGG 0.153196 2.2:+21025560 None:intergenic
GTTAAGTACTTAATATTAAA+TGG 0.208632 2.2:-21023975 MS.gene068654:CDS
CCTCACACTCTAGGTCAATT+TGG 0.235008 2.2:-21024144 MS.gene068654:intron
TTCTGAATAATATTACAATT+TGG 0.250934 2.2:-21023947 MS.gene068654:CDS
CTTACCCTGGAAGTTTGTTT+TGG 0.254096 2.2:-21024088 MS.gene068654:CDS
CAATATGGCTGAAGCAATAA+TGG 0.261497 2.2:-21026504 None:intergenic
TGTGAACGAGCGTAGGATTT+TGG 0.266869 2.2:-21025377 MS.gene068654:CDS
CCTAAAGAATGTTTGTATAA+AGG 0.274042 2.2:-21024030 MS.gene068654:CDS
CCTTTATACAAACATTCTTT+AGG 0.276237 2.2:+21024030 None:intergenic
TGAAATTTGTGGTGGCATTT+TGG 0.276341 2.2:-21025182 MS.gene068654:intron
GATTCTTGTGATTATCGTTA+TGG 0.307940 2.2:-21025700 MS.gene068654:CDS
GCACGTAGAACCCGAAGTTT+TGG 0.308366 2.2:+21025280 None:intergenic
CTAAAGAATGTTTGTATAAA+GGG 0.314320 2.2:-21024029 MS.gene068654:CDS
CAAAATCTGTTGAGGATTAA+GGG 0.329839 2.2:+21025561 None:intergenic
TTCAGCCAAAACAAACTTCC+AGG 0.338704 2.2:+21024083 None:intergenic
ATAGAGTATATAAGAAAATA+TGG 0.344916 2.2:-21023825 MS.gene068654:CDS
TTTGTCTTTCGTCAAAATTA+AGG 0.347473 2.2:+21026312 None:intergenic
TTCAGATCAATGGATAATTT+CGG 0.351106 2.2:+21023855 None:intergenic
TACAATAATGTTCAAATGCT+TGG 0.353231 2.2:-21025256 MS.gene068654:CDS
AACTTCAATCTTGCAGTGAC+TGG 0.362551 2.2:-21026245 MS.gene068654:CDS
CTTAGAGGCCCCAAAACTTC+GGG 0.365917 2.2:-21025290 MS.gene068654:CDS
TTGTGCAAGTGGGGATGTTG+TGG 0.396215 2.2:-21025776 MS.gene068654:CDS
AGGGATATTATTGAAATTTG+TGG 0.398913 2.2:-21025193 MS.gene068654:CDS
TGTGCAAGTGGGGATGTTGT+GGG 0.402090 2.2:-21025775 MS.gene068654:CDS
CAACTCAAGAACCTTAAAAC+TGG 0.403099 2.2:+21025612 None:intergenic
TTATTTGAAGATTCATTAGT+TGG 0.403722 2.2:+21024255 None:intergenic
TGCAAGATTGAAGTTTAGTC+TGG 0.413386 2.2:+21026253 None:intergenic
TCATGAAGATCTGATCTTAG+AGG 0.415542 2.2:-21025305 MS.gene068654:CDS
TCAAATATCGACAATCATAT+TGG 0.415878 2.2:-21025838 MS.gene068654:CDS
TATTTGAAGATTCATTAGTT+GGG 0.418765 2.2:+21024256 None:intergenic
TACAAACATTCTTTAGGTTG+TGG 0.433648 2.2:+21024036 None:intergenic
ATGGTGGTAGCTTTGATGAT+TGG 0.451451 2.2:-21023806 MS.gene068654:CDS
CCACAATGATTCCCATCGCT+TGG 0.455748 2.2:+21026349 None:intergenic
TGGTGGTAGCTTTGATGATT+GGG 0.457946 2.2:-21023805 MS.gene068654:CDS
GTTCTCACGACTTCCGTGTA+AGG 0.459091 2.2:+21026383 None:intergenic
GGGATGTTGTGGGGTGGATG+AGG 0.463671 2.2:-21025765 MS.gene068654:CDS
GCATTCCTTTCAATCATGTC+TGG 0.464346 2.2:+21025670 None:intergenic
GATAATTTCGGAGAGAGCTT+CGG 0.466406 2.2:+21023867 None:intergenic
TATCCCAGGTTTCAGATCAA+TGG 0.467659 2.2:+21023845 None:intergenic
TCTAATGCTACAACTCTTCA+AGG 0.468328 2.2:+21025813 None:intergenic
ATATAAGCTAGAAGTGCATC+AGG 0.471803 2.2:+21026419 None:intergenic
TCCCCACTTGCACAACTCTC+AGG 0.473005 2.2:+21025784 None:intergenic
TTATCCATTGATCTGAAACC+TGG 0.478986 2.2:-21023849 MS.gene068654:CDS
ACGACTTCCGTGTAAGGAAG+TGG 0.493916 2.2:+21026389 None:intergenic
AAACTGGAGAAAACTTCAAA+AGG 0.495741 2.2:+21025628 None:intergenic
CATTTCTCCACTTCCTTACA+CGG 0.495992 2.2:-21026396 MS.gene068654:CDS
AGGGACACAAATCAGAGTTA+TGG 0.497222 2.2:-21026471 MS.gene068654:CDS
GTGCAAGTGGGGATGTTGTG+GGG 0.497345 2.2:-21025774 MS.gene068654:CDS
TCTTAGAGGCCCCAAAACTT+CGG 0.498004 2.2:-21025291 MS.gene068654:CDS
CAAGTGGGGATGTTGTGGGG+TGG 0.508240 2.2:-21025771 MS.gene068654:CDS
CAAAACAAACTTCCAGGGTA+AGG 0.518602 2.2:+21024089 None:intergenic
CTTTCGTCAAAATTAAGGCT+AGG 0.519973 2.2:+21026317 None:intergenic
AATATGGCTGAAGCAATAAT+GGG 0.521425 2.2:-21026503 MS.gene068654:CDS
TTAAGAAATCACATTTCTCA+AGG 0.522132 2.2:+21025452 None:intergenic
GCAATAATGGGTGAAGGAGA+AGG 0.522729 2.2:-21026491 MS.gene068654:CDS
TCAATTCAAAATCGAGGCAA+AGG 0.529641 2.2:+21024327 None:intergenic
TTTCTTATATACTCTATCCC+AGG 0.537281 2.2:+21023831 None:intergenic
TATCCATTGATCTGAAACCT+GGG 0.540917 2.2:-21023848 MS.gene068654:CDS
ATTTGAAGATTCATTAGTTG+GGG 0.541423 2.2:+21024257 None:intergenic
TGCAGTGACTGGGCAACGTA+AGG 0.547546 2.2:-21026234 MS.gene068654:intron
CAATTCAAAATCGAGGCAAA+GGG 0.555813 2.2:+21024328 None:intergenic
CAAGTGTGCTCTCCAAGCGA+TGG 0.562082 2.2:-21026361 MS.gene068654:CDS
AACTTCAAAAGGTAAATCAA+TGG 0.562125 2.2:+21025639 None:intergenic
GATATTATTGAAATTTGTGG+TGG 0.564526 2.2:-21025190 MS.gene068654:CDS
TACCGTTCAATTCAAAATCG+AGG 0.564943 2.2:+21024321 None:intergenic
ACTTCAATCTTGCAGTGACT+GGG 0.567133 2.2:-21026244 MS.gene068654:CDS
ATGTGATTTCTTAACAGATG+AGG 0.570169 2.2:-21025443 MS.gene068654:CDS
GTTTGTATAAAGGGATACAA+AGG 0.571149 2.2:-21024020 MS.gene068654:CDS
TCAGCCAAAACAAACTTCCA+GGG 0.571326 2.2:+21024084 None:intergenic
TCTTGTGATTATCGTTATGG+AGG 0.571564 2.2:-21025697 MS.gene068654:CDS
TTTGGTTTCTGCATTTAACA+TGG 0.582670 2.2:-21025359 MS.gene068654:CDS
GAATACCAGACATGATTGAA+AGG 0.585171 2.2:-21025675 MS.gene068654:CDS
AAGTGTGCTCTCCAAGCGAT+GGG 0.588507 2.2:-21026360 MS.gene068654:CDS
TTTGTGATGTGAACGAGCGT+AGG 0.592653 2.2:-21025384 MS.gene068654:CDS
CAATAATGGGTGAAGGAGAA+GGG 0.594550 2.2:-21026490 MS.gene068654:CDS
GGTCATCGAGGTTCTAGCAT+GGG 0.596942 2.2:-21024374 MS.gene068654:CDS
GAATCTTCAAATAAATAACC+AGG 0.602091 2.2:-21024246 MS.gene068654:intron
TACTATGATCAAAATGACAA+TGG 0.603700 2.2:-21024119 MS.gene068654:CDS
CCAAGCGATGGGAATCATTG+TGG 0.604799 2.2:-21026349 MS.gene068654:CDS
GACACAAATCAGAGTTATGG+AGG 0.609354 2.2:-21026468 MS.gene068654:CDS
AAGTACTTAATATTAAATGG+AGG 0.612207 2.2:-21023972 MS.gene068654:CDS
ACAGGTAGATCTTGAAAGTG+AGG 0.613723 2.2:-21025893 MS.gene068654:intron
GATATTTGATGTAACCATCA+TGG 0.615010 2.2:+21025852 None:intergenic
TGTATAAAGGGATACAAAGG+TGG 0.615243 2.2:-21024017 MS.gene068654:CDS
TTGTGGCTGACACACCTACA+CGG 0.616160 2.2:+21024053 None:intergenic
AATGGCTGTCTTCCTTACCC+TGG 0.616901 2.2:-21024101 MS.gene068654:CDS
ATCTGTTGAGGATTAAGGGA+GGG 0.619665 2.2:+21025565 None:intergenic
AGAGTATTCAAAATCTGTTG+AGG 0.620364 2.2:+21025553 None:intergenic
TGCCTGAGAGTTGTGCAAGT+GGG 0.620485 2.2:-21025786 MS.gene068654:CDS
GCCTGAGAGTTGTGCAAGTG+GGG 0.621594 2.2:-21025785 MS.gene068654:CDS
CAAGAGATGAACAATGAGAG+AGG 0.622832 2.2:+21025485 None:intergenic
GCTGAAGCAATAATGGGTGA+AGG 0.623645 2.2:-21026497 MS.gene068654:CDS
GGGTCATCGAGGTTCTAGCA+TGG 0.629061 2.2:-21024375 MS.gene068654:CDS
TGTGGCTGACACACCTACAC+GGG 0.629776 2.2:+21024054 None:intergenic
CTAATGCTACAACTCTTCAA+GGG 0.636034 2.2:+21025814 None:intergenic
GGCTGAAAAGAGACCCGTGT+AGG 0.671792 2.2:-21024067 MS.gene068654:CDS
TTGCCTGAGAGTTGTGCAAG+TGG 0.675075 2.2:-21025787 MS.gene068654:CDS
GAGTATATAAGAAAATATGG+TGG 0.676614 2.2:-21023822 MS.gene068654:CDS
TGATTGTCTTAGGGTCATCG+AGG 0.687412 2.2:-21024386 MS.gene068654:intron
AATCTGTTGAGGATTAAGGG+AGG 0.688199 2.2:+21025564 None:intergenic
TTTGAAGATTCATTAGTTGG+GGG 0.726871 2.2:+21024258 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATAATTTATATA+TGG - chr2.2:21025284-21025303 MS.gene068654:CDS 0.0%
!! AATTCTTTATATAAATTCTT+TGG + chr2.2:21025797-21025816 None:intergenic 10.0%
!! TCAAATATGATATATATTAT+TGG - chr2.2:21024360-21024379 MS.gene068654:CDS 10.0%
!! ATAGAGTATATAAGAAAATA+TGG - chr2.2:21026463-21026482 MS.gene068654:CDS 15.0%
!! ATGATATCTATTTAATACTT+AGG - chr2.2:21025543-21025562 MS.gene068654:CDS 15.0%
!! GTTAAGTACTTAATATTAAA+TGG - chr2.2:21026313-21026332 MS.gene068654:CDS 15.0%
!! TAGATGAACTTTAATTATAA+AGG - chr2.2:21024333-21024352 MS.gene068654:CDS 15.0%
!! TTCTGAATAATATTACAATT+TGG - chr2.2:21026341-21026360 MS.gene068654:CDS 15.0%
!!! CACAAATTTTAATATATTTG+AGG + chr2.2:21024890-21024909 None:intergenic 15.0%
!!! CATCTATTTTATTAGAAAAA+TGG + chr2.2:21024319-21024338 None:intergenic 15.0%
!!! TAATATTACAATTTGGTTTT+TGG - chr2.2:21026348-21026367 MS.gene068654:CDS 15.0%
!!! TATATAAAGAATTGAAGTTT+TGG - chr2.2:21025804-21025823 MS.gene068654:CDS 15.0%
!!! TGTTTTTTACTATAAACTTT+AGG - chr2.2:21024111-21024130 MS.gene068654:CDS 15.0%
!! AAATAATAGATACTCATGAT+AGG + chr2.2:21025738-21025757 None:intergenic 20.0%
!! AAGTACTTAATATTAAATGG+AGG - chr2.2:21026316-21026335 MS.gene068654:CDS 20.0%
!! AGTTCATAGCTTATTATATA+TGG - chr2.2:21025478-21025497 MS.gene068654:CDS 20.0%
!! ATCAAACAGTTCATATTTAA+TGG - chr2.2:21024244-21024263 MS.gene068654:intron 20.0%
!! CATTTCTTTATAGTTAATAG+AGG - chr2.2:21025191-21025210 MS.gene068654:CDS 20.0%
!! CTAAAGAATGTTTGTATAAA+GGG - chr2.2:21026259-21026278 MS.gene068654:CDS 20.0%
!! TAATCAATATTTGTAGCATA+AGG + chr2.2:21025630-21025649 None:intergenic 20.0%
!! TATTTGAAGATTCATTAGTT+GGG + chr2.2:21026035-21026054 None:intergenic 20.0%
!! TTATTTGAAGATTCATTAGT+TGG + chr2.2:21026036-21026055 None:intergenic 20.0%
!!! AATAGTAATACTACATTTTG+AGG + chr2.2:21025836-21025855 None:intergenic 20.0%
!!! AGGTTTTATAAATAGTCAAT+AGG + chr2.2:21025606-21025625 None:intergenic 20.0%
!!! ATTTTAATATATTTGAGGCT+TGG + chr2.2:21024885-21024904 None:intergenic 20.0%
!!! GGTTTTATAAATAGTCAATA+GGG + chr2.2:21025605-21025624 None:intergenic 20.0%
!!! TATATTTTCTGATTGTCTTA+GGG - chr2.2:21025893-21025912 MS.gene068654:intron 20.0%
!!! TTATATTTTCTGATTGTCTT+AGG - chr2.2:21025892-21025911 MS.gene068654:CDS 20.0%
! AACTTCAAAAGGTAAATCAA+TGG + chr2.2:21024652-21024671 None:intergenic 25.0%
! ACTAATTGAGTATTTGTGAT+AGG - chr2.2:21025501-21025520 MS.gene068654:CDS 25.0%
! AGGGATATTATTGAAATTTG+TGG - chr2.2:21025095-21025114 MS.gene068654:intron 25.0%
! ATTTGAAGATTCATTAGTTG+GGG + chr2.2:21026034-21026053 None:intergenic 25.0%
! CCTAAAGAATGTTTGTATAA+AGG - chr2.2:21026258-21026277 MS.gene068654:CDS 25.0%
! CCTTTATACAAACATTCTTT+AGG + chr2.2:21026261-21026280 None:intergenic 25.0%
! GAATCTTCAAATAAATAACC+AGG - chr2.2:21026042-21026061 MS.gene068654:intron 25.0%
! GAGTATATAAGAAAATATGG+TGG - chr2.2:21026466-21026485 MS.gene068654:CDS 25.0%
! GATATTATTGAAATTTGTGG+TGG - chr2.2:21025098-21025117 MS.gene068654:intron 25.0%
! TACAATAATGTTCAAATGCT+TGG - chr2.2:21025032-21025051 MS.gene068654:intron 25.0%
! TACTATGATCAAAATGACAA+TGG - chr2.2:21026169-21026188 MS.gene068654:intron 25.0%
! TATTTAATGGAGAATGAACT+AGG - chr2.2:21024257-21024276 MS.gene068654:CDS 25.0%
! TCAAATATCGACAATCATAT+TGG - chr2.2:21024450-21024469 MS.gene068654:intron 25.0%
! TTAAGAAATCACATTTCTCA+AGG + chr2.2:21024839-21024858 None:intergenic 25.0%
! TTCAGATCAATGGATAATTT+CGG + chr2.2:21026436-21026455 None:intergenic 25.0%
! TTTGTCTTTCGTCAAAATTA+AGG + chr2.2:21023979-21023998 None:intergenic 25.0%
!! TAAGCTATGAACTTTTTGTA+TGG + chr2.2:21025471-21025490 None:intergenic 25.0%
!! TATTGGATTTTATGCATAAC+AGG - chr2.2:21024377-21024396 MS.gene068654:CDS 25.0%
!!! GGACAAAATGTTTTTATAGA+TGG - chr2.2:21025053-21025072 MS.gene068654:intron 25.0%
!!! GGGAAGTATCTTTAAAAATT+TGG - chr2.2:21025934-21025953 MS.gene068654:intron 25.0%
!!! TTTTTTGTGAAGATTAACCA+TGG + chr2.2:21024198-21024217 None:intergenic 25.0%
AAACTGGAGAAAACTTCAAA+AGG + chr2.2:21024663-21024682 None:intergenic 30.0%
AGCCAAAAAATCAAATTGCA+TGG + chr2.2:21025668-21025687 None:intergenic 30.0%
ATGTGATTTCTTAACAGATG+AGG - chr2.2:21024845-21024864 MS.gene068654:intron 30.0%
CAAAATCTGTTGAGGATTAA+GGG + chr2.2:21024730-21024749 None:intergenic 30.0%
CAATATTTGTAGCATAAGGA+AGG + chr2.2:21025626-21025645 None:intergenic 30.0%
CACAAAAAATCACGCTTATT+GGG - chr2.2:21025438-21025457 MS.gene068654:CDS 30.0%
GATATTTGATGTAACCATCA+TGG + chr2.2:21024439-21024458 None:intergenic 30.0%
GATTCTTGTGATTATCGTTA+TGG - chr2.2:21024588-21024607 MS.gene068654:intron 30.0%
GTTTGTATAAAGGGATACAA+AGG - chr2.2:21026268-21026287 MS.gene068654:CDS 30.0%
TCAAAATCTGTTGAGGATTA+AGG + chr2.2:21024731-21024750 None:intergenic 30.0%
TCACAAAAAATCACGCTTAT+TGG - chr2.2:21025437-21025456 MS.gene068654:CDS 30.0%
TTTCTTATATACTCTATCCC+AGG + chr2.2:21026460-21026479 None:intergenic 30.0%
TTTGAAGATTCATTAGTTGG+GGG + chr2.2:21026033-21026052 None:intergenic 30.0%
! AGAGTATTCAAAATCTGTTG+AGG + chr2.2:21024738-21024757 None:intergenic 30.0%
! TGAAGTTTTCTCCAGTTTTA+AGG - chr2.2:21024665-21024684 MS.gene068654:intron 30.0%
! TTTGGTTTCTGCATTTAACA+TGG - chr2.2:21024929-21024948 MS.gene068654:intron 30.0%
!! TACAAACATTCTTTAGGTTG+TGG + chr2.2:21026255-21026274 None:intergenic 30.0%
!!! ATAAGCGTGATTTTTTGTGA+TGG + chr2.2:21025437-21025456 None:intergenic 30.0%
!!! ATTTTTTGTGATGGCTCAAA+AGG + chr2.2:21025428-21025447 None:intergenic 30.0%
!!! CTCCATGCAATTTGATTTTT+TGG - chr2.2:21025663-21025682 MS.gene068654:CDS 30.0%
!!! TTTTTTGTGATGGCTCAAAA+GGG + chr2.2:21025427-21025446 None:intergenic 30.0%
ACTCGAAAAAGAGGTGATTA+AGG - chr2.2:21024551-21024570 MS.gene068654:intron 35.0%
AGGGAATCATACATGCATTA+AGG + chr2.2:21025408-21025427 None:intergenic 35.0%
CAACTCAAGAACCTTAAAAC+TGG + chr2.2:21024679-21024698 None:intergenic 35.0%
CAATTCAAAATCGAGGCAAA+GGG + chr2.2:21025963-21025982 None:intergenic 35.0%
CTAATGCTACAACTCTTCAA+GGG + chr2.2:21024477-21024496 None:intergenic 35.0%
CTTTCGTCAAAATTAAGGCT+AGG + chr2.2:21023974-21023993 None:intergenic 35.0%
GAATACCAGACATGATTGAA+AGG - chr2.2:21024613-21024632 MS.gene068654:intron 35.0%
GAGGAAATTACTCGAAAAAG+AGG - chr2.2:21024542-21024561 MS.gene068654:intron 35.0%
TACCGTTCAATTCAAAATCG+AGG + chr2.2:21025970-21025989 None:intergenic 35.0%
TACTTAGGATGAACACAGAT+TGG - chr2.2:21025558-21025577 MS.gene068654:CDS 35.0%
TCAATTCAAAATCGAGGCAA+AGG + chr2.2:21025964-21025983 None:intergenic 35.0%
TCATGAAGATCTGATCTTAG+AGG - chr2.2:21024983-21025002 MS.gene068654:intron 35.0%
TCTAATGCTACAACTCTTCA+AGG + chr2.2:21024478-21024497 None:intergenic 35.0%
TCTTGTGATTATCGTTATGG+AGG - chr2.2:21024591-21024610 MS.gene068654:intron 35.0%
TGCAAGATTGAAGTTTAGTC+TGG + chr2.2:21024038-21024057 None:intergenic 35.0%
TGCTGCATTACAATTCAACT+AGG - chr2.2:21025241-21025260 MS.gene068654:CDS 35.0%
TGTATAAAGGGATACAAAGG+TGG - chr2.2:21026271-21026290 MS.gene068654:CDS 35.0%
TTCCAAGTGAAGTATAGTAG+AGG + chr2.2:21025141-21025160 None:intergenic 35.0%
TTCCTCTACTATACTTCACT+TGG - chr2.2:21025136-21025155 MS.gene068654:intron 35.0%
! ATATAAGCTAGAAGTGCATC+AGG + chr2.2:21023872-21023891 None:intergenic 35.0%
! ATGCAAGCTTTTGACAACAA+GGG - chr2.2:21025076-21025095 MS.gene068654:intron 35.0%
! TATCCATTGATCTGAAACCT+GGG - chr2.2:21026440-21026459 MS.gene068654:CDS 35.0%
! TGAAATTTGTGGTGGCATTT+TGG - chr2.2:21025106-21025125 MS.gene068654:intron 35.0%
! TGCCTCGATTTTGAATTGAA+CGG - chr2.2:21025965-21025984 MS.gene068654:intron 35.0%
! TTATCCATTGATCTGAAACC+TGG - chr2.2:21026439-21026458 MS.gene068654:CDS 35.0%
!! AATAGTCAATAGGGTGTTGA+TGG + chr2.2:21025596-21025615 None:intergenic 35.0%
AACTTCAATCTTGCAGTGAC+TGG - chr2.2:21024043-21024062 MS.gene068654:CDS 40.0%
AATCTGTTGAGGATTAAGGG+AGG + chr2.2:21024727-21024746 None:intergenic 40.0%
ACAGGTAGATCTTGAAAGTG+AGG - chr2.2:21024395-21024414 MS.gene068654:intron 40.0%
ACATTTAACCCTCACACTCT+AGG - chr2.2:21026135-21026154 MS.gene068654:intron 40.0%
ACTTCAATCTTGCAGTGACT+GGG - chr2.2:21024044-21024063 MS.gene068654:CDS 40.0%
AGGGACACAAATCAGAGTTA+TGG - chr2.2:21023817-21023836 MS.gene068654:CDS 40.0%
ATAACCAGGTATGCTTGCTT+AGG - chr2.2:21026056-21026075 MS.gene068654:intron 40.0%
ATCTGTTGAGGATTAAGGGA+GGG + chr2.2:21024726-21024745 None:intergenic 40.0%
CAAAACAAACTTCCAGGGTA+AGG + chr2.2:21026202-21026221 None:intergenic 40.0%
CAAATTGACCTAGAGTGTGA+GGG + chr2.2:21026146-21026165 None:intergenic 40.0%
CAAGAGATGAACAATGAGAG+AGG + chr2.2:21024806-21024825 None:intergenic 40.0%
CATTTCTCCACTTCCTTACA+CGG - chr2.2:21023892-21023911 MS.gene068654:CDS 40.0%
CTATATCTCTAGACAAGCCA+TGG - chr2.2:21024178-21024197 MS.gene068654:intron 40.0%
GACACAAATCAGAGTTATGG+AGG - chr2.2:21023820-21023839 MS.gene068654:CDS 40.0%
GATAATTTCGGAGAGAGCTT+CGG + chr2.2:21026424-21026443 None:intergenic 40.0%
GCATTCCTTTCAATCATGTC+TGG + chr2.2:21024621-21024640 None:intergenic 40.0%
GGCTTGTCTAGAGATATAGA+GGG + chr2.2:21024177-21024196 None:intergenic 40.0%
TAATCCTAAGCAAGCATACC+TGG + chr2.2:21026063-21026082 None:intergenic 40.0%
TATCCCAGGTTTCAGATCAA+TGG + chr2.2:21026446-21026465 None:intergenic 40.0%
TCAGCCAAAACAAACTTCCA+GGG + chr2.2:21026207-21026226 None:intergenic 40.0%
TTCAGCCAAAACAAACTTCC+AGG + chr2.2:21026208-21026227 None:intergenic 40.0%
! GATGCAAGCTTTTGACAACA+AGG - chr2.2:21025075-21025094 MS.gene068654:intron 40.0%
! TGGCTTGTCTAGAGATATAG+AGG + chr2.2:21024178-21024197 None:intergenic 40.0%
!! ATGGTGGTAGCTTTGATGAT+TGG - chr2.2:21026482-21026501 MS.gene068654:CDS 40.0%
!! CAATAATGGGTGAAGGAGAA+GGG - chr2.2:21023798-21023817 MS.gene068654:CDS 40.0%
!! CTTACCCTGGAAGTTTGTTT+TGG - chr2.2:21026200-21026219 MS.gene068654:intron 40.0%
!! GTTTTTGGATGACTGCTCAT+GGG - chr2.2:21026363-21026382 MS.gene068654:CDS 40.0%
!! TGGTGGTAGCTTTGATGATT+GGG - chr2.2:21026483-21026502 MS.gene068654:CDS 40.0%
AAGATTCATTAGTTGGGGGC+AGG + chr2.2:21026029-21026048 None:intergenic 45.0%
CCAAATTGACCTAGAGTGTG+AGG + chr2.2:21026147-21026166 None:intergenic 45.0%
CCTCACACTCTAGGTCAATT+TGG - chr2.2:21026144-21026163 MS.gene068654:intron 45.0%
TCTTAGAGGCCCCAAAACTT+CGG - chr2.2:21024997-21025016 MS.gene068654:intron 45.0%
TGATTGTCTTAGGGTCATCG+AGG - chr2.2:21025902-21025921 MS.gene068654:intron 45.0%
TTTGTGATGTGAACGAGCGT+AGG - chr2.2:21024904-21024923 MS.gene068654:intron 45.0%
! ACGTAGAACCCGAAGTTTTG+GGG + chr2.2:21025009-21025028 None:intergenic 45.0%
! CACGTAGAACCCGAAGTTTT+GGG + chr2.2:21025010-21025029 None:intergenic 45.0%
! TGTGAACGAGCGTAGGATTT+TGG - chr2.2:21024911-21024930 MS.gene068654:intron 45.0%
! TTTTGCTGAAGCAGCCATGA+TGG - chr2.2:21024422-21024441 MS.gene068654:intron 45.0%
!! GCAATAATGGGTGAAGGAGA+AGG - chr2.2:21023797-21023816 MS.gene068654:CDS 45.0%
!! GCTGAAGCAATAATGGGTGA+AGG - chr2.2:21023791-21023810 MS.gene068654:CDS 45.0%
!! GGTTTTTGGATGACTGCTCA+TGG - chr2.2:21026362-21026381 MS.gene068654:CDS 45.0%
AAGTGTGCTCTCCAAGCGAT+GGG - chr2.2:21023928-21023947 MS.gene068654:CDS 50.0%
AATGGCTGTCTTCCTTACCC+TGG - chr2.2:21026187-21026206 MS.gene068654:intron 50.0%
ACGACTTCCGTGTAAGGAAG+TGG + chr2.2:21023902-21023921 None:intergenic 50.0%
CCAAGCGATGGGAATCATTG+TGG - chr2.2:21023939-21023958 MS.gene068654:CDS 50.0%
CCACAATGATTCCCATCGCT+TGG + chr2.2:21023942-21023961 None:intergenic 50.0%
CTTAGAGGCCCCAAAACTTC+GGG - chr2.2:21024998-21025017 MS.gene068654:intron 50.0%
GTTCTCACGACTTCCGTGTA+AGG + chr2.2:21023908-21023927 None:intergenic 50.0%
TGCCTGAGAGTTGTGCAAGT+GGG - chr2.2:21024502-21024521 MS.gene068654:intron 50.0%
TGTGCAAGTGGGGATGTTGT+GGG - chr2.2:21024513-21024532 MS.gene068654:intron 50.0%
TTGCCTGAGAGTTGTGCAAG+TGG - chr2.2:21024501-21024520 MS.gene068654:intron 50.0%
TTGTGCAAGTGGGGATGTTG+TGG - chr2.2:21024512-21024531 MS.gene068654:intron 50.0%
TTGTGGCTGACACACCTACA+CGG + chr2.2:21026238-21026257 None:intergenic 50.0%
! GCACGTAGAACCCGAAGTTT+TGG + chr2.2:21025011-21025030 None:intergenic 50.0%
! GGTCATCGAGGTTCTAGCAT+GGG - chr2.2:21025914-21025933 MS.gene068654:intron 50.0%
!! TAGGGTGTTGATGGCGTACT+AGG + chr2.2:21025587-21025606 None:intergenic 50.0%
CAAGTGTGCTCTCCAAGCGA+TGG - chr2.2:21023927-21023946 MS.gene068654:CDS 55.0%
GCCTGAGAGTTGTGCAAGTG+GGG - chr2.2:21024503-21024522 MS.gene068654:intron 55.0%
GGCTGAAAAGAGACCCGTGT+AGG - chr2.2:21026221-21026240 MS.gene068654:intron 55.0%
GTGCAAGTGGGGATGTTGTG+GGG - chr2.2:21024514-21024533 MS.gene068654:intron 55.0%
TCCCCACTTGCACAACTCTC+AGG + chr2.2:21024507-21024526 None:intergenic 55.0%
TGCAGTGACTGGGCAACGTA+AGG - chr2.2:21024054-21024073 MS.gene068654:CDS 55.0%
TGTGGCTGACACACCTACAC+GGG + chr2.2:21026237-21026256 None:intergenic 55.0%
! GGGTCATCGAGGTTCTAGCA+TGG - chr2.2:21025913-21025932 MS.gene068654:intron 55.0%
CAAGTGGGGATGTTGTGGGG+TGG - chr2.2:21024517-21024536 MS.gene068654:intron 60.0%
GGGATGTTGTGGGGTGGATG+AGG - chr2.2:21024523-21024542 MS.gene068654:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 21023788 21026522 21023788 ID=MS.gene068654
chr2.2 mRNA 21023788 21026522 21023788 ID=MS.gene068654.t1;Parent=MS.gene068654
chr2.2 exon 21026235 21026522 21026235 ID=MS.gene068654.t1.exon1;Parent=MS.gene068654.t1
chr2.2 CDS 21026235 21026522 21026235 ID=cds.MS.gene068654.t1;Parent=MS.gene068654.t1
chr2.2 exon 21025183 21025911 21025183 ID=MS.gene068654.t1.exon2;Parent=MS.gene068654.t1
chr2.2 CDS 21025183 21025911 21025183 ID=cds.MS.gene068654.t1;Parent=MS.gene068654.t1
chr2.2 exon 21024247 21024396 21024247 ID=MS.gene068654.t1.exon3;Parent=MS.gene068654.t1
chr2.2 CDS 21024247 21024396 21024247 ID=cds.MS.gene068654.t1;Parent=MS.gene068654.t1
chr2.2 exon 21023788 21024153 21023788 ID=MS.gene068654.t1.exon4;Parent=MS.gene068654.t1
chr2.2 CDS 21023788 21024153 21023788 ID=cds.MS.gene068654.t1;Parent=MS.gene068654.t1
Gene Sequence

>MS.gene068654

ATGGCTGAAGCAATAATGGGTGAAGGAGAAGGGACACAAATCAGAGTTATGGAGGAAGAAGAAGATTTGATCAGCAATCTTCCTGATGCACTTCTAGCTTATATCATTTCTCCACTTCCTTACACGGAAGTCGTGAGAACAAGTGTGCTCTCCAAGCGATGGGAATCATTGTGGAAACACACTCCTAGCCTTAATTTTGACGAAAGACAAATGTTAAAGTCATTGATCAAAGAAAACAATCAAAATTCCAGACTAAACTTCAATCTTGCAGTGACTGGGCAACGTAAGGTACATTGTTATAAAAATTTATGTAAAATTTTATGTGTTTTTTACTATAAACTTTAGGTTTTAATTTATGATTTTTTTTTTTGTTGATCGTGTCTAGTCCCTCTATATCTCTAGACAAGCCATGGTTAATCTTCACAAAAAATTATAGATATCTTAATAATTAGATTAATCAAACAGTTCATATTTAATGGAGAATGAACTAGGAAAAATTAGAGAGAATATGATTACATATTTTAGCTTCCATTTTTCTAATAAAATAGATGAACTTTAATTATAAAGGCTTTTCAAATATGATATATATTATTGGATTTTATGCATAACAGGTAGATCTTGAAAGTGAGGAACTTTTTGCTGAAGCAGCCATGATGGTTACATCAAATATCGACAATCATATTGGTCCCTTGAAGAGTTGTAGCATTAGACACTTGCCTGAGAGTTGTGCAAGTGGGGATGTTGTGGGGTGGATGAGGAAATTACTCGAAAAAGAGGTGATTAAGGTTTCAATAGAACGTGATTCTTGTGATTATCGTTATGGAGGAATACCAGACATGATTGAAAGGAATGCTGCATCAACCATTGATTTACCTTTTGAAGTTTTCTCCAGTTTTAAGGTTCTTGAGTTGAAGAACTATCATTTTTTAACTGCACCCTCCCTTAATCCTCAACAGATTTTGAATACTCTTACTCTTAACAAAGTGCATATTATATCTAACACTTTTCATGATATCCTCTCTCATTGTTCATCTCTTGAAAATCTTATCCTTGAGAAATGTGATTTCTTAACAGATGAGGTTAAGATTGTAAGTCCAAGCCTCAAATATATTAAAATTTGTGATGTGAACGAGCGTAGGATTTTGGTTTCTGCATTTAACATGGAAGTCATAGAGATTGACTCTGTTACTTGTAATCATGAAGATCTGATCTTAGAGGCCCCAAAACTTCGGGTTCTACGTGCTTACAATAATGTTCAAATGCTTGGACAAAATGTTTTTATAGATGGATGCAAGCTTTTGACAACAAGGGATATTATTGAAATTTGTGGTGGCATTTTGGTAAGTGTTTCCTCTACTATACTTCACTTGGAATGTCAGTTTTTTTGATTGATGCATATATTACATTTCTTTATAGTTAATAGAGGTGCATGTAATCTAATGTTTTAAATTTTTGCTGCATTACAATTCAACTAGGATATATGTGTGTGTCTATATATATATATATAATTTATATATGGTTCATATTCAATTACAATTGAAGATTACAATCATATGTACATAGATGCAATCATTAAGATTAATACGTGAGTCAATGATAATGATTAAACTTTAATATCCTTAATGCATGTATGATTCCCTTTTGAGCCATCACAAAAAATCACGCTTATTGGGCAATACTCCATACAAAAAGTTCATAGCTTATTATATATGGACTAATTGAGTATTTGTGATAGGATAATAAAACTTGTTGTTTATGATATCTATTTAATACTTAGGATGAACACAGATTGGTAACCTAGTACGCCATCAACACCCTATTGACTATTTATAAAACCTTCCTTATGCTACAAATATTGATTAGAGTTAGAAAACTCTCCATGCAATTTGATTTTTTGGCTTAGAAATATGTCATGTAAAAAATAGACAATTATATTTTAGTTTTTGACCTATCATGAGTATCTATTATTTAGATCGTTCAGTAATGCTAATTAAATTTTACAGCAACCAAAGAATTTATATAAAGAATTGAAGTTTTGGTTGTATCCTCAAAATGTAGTATTACTATTATAATTTATGAACATTTATGTCTATTTGTTTTATAATTATATTTTCTGATTGTCTTAGGGTCATCGAGGTTCTAGCATGGGAAGTATCTTTAAAAATTTGGTTACCCTTTGCCTCGATTTTGAATTGAACGGTAATAGAAATGTCATAGCTCTTTCTTTTGCACTCAAGTCCTGCCCCCAACTAATGAATCTTCAAATAAATAACCAGGTATGCTTGCTTAGGATTATTTTAGCTTGATCATGATTGCAGTCATTATACTTAACTATATATATTTTTTGACATTTAACCCTCACACTCTAGGTCAATTTGGATTACTATGATCAAAATGACAATGGCTGTCTTCCTTACCCTGGAAGTTTGTTTTGGCTGAAAAGAGACCCGTGTAGGTGTGTCAGCCACAACCTAAAGAATGTTTGTATAAAGGGATACAAAGGTGGAGAGTTTGAAATTGAATTTGTTAAGTACTTAATATTAAATGGAGGAGTTCTGAATAATATTACAATTTGGTTTTTGGATGACTGCTCATGGGATGAAGTCATAGCAACTGCATGTTTACAGTCATATCCGAAGCTCTCTCCGAAATTATCCATTGATCTGAAACCTGGGATAGAGTATATAAGAAAATATGGTGGTAGCTTTGATGATTGGGTCCGAACCCTAAAATAA
Protein sequence

>MS.gene068654.t1

MAEAIMGEGEGTQIRVMEEEEDLISNLPDALLAYIISPLPYTEVVRTSVLSKRWESLWKHTPSLNFDERQMLKSLIKENNQNSRLNFNLAVTGQRKVDLESEELFAEAAMMVTSNIDNHIGPLKSCSIRHLPESCASGDVVGWMRKLLEKEVIKVSIERDSCDYRYGGIPDMIERNAASTIDLPFEVFSSFKVLELKNYHFLTAPSLNPQQILNTLTLNKVHIISNTFHDILSHCSSLENLILEKCDFLTDEVKIVSPSLKYIKICDVNERRILVSAFNMEVIEIDSVTCNHEDLILEAPKLRVLRAYNNVQMLGQNVFIDGCKLLTTRDIIEICGGILGHRGSSMGSIFKNLVTLCLDFELNGNRNVIALSFALKSCPQLMNLQINNQVNLDYYDQNDNGCLPYPGSLFWLKRDPCRCVSHNLKNVCIKGYKGGEFEIEFVKYLILNGGVLNNITIWFLDDCSWDEVIATACLQSYPKLSPKLSIDLKPGIEYIRKYGGSFDDWVRTLK