AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068655

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068655.t1 MTR_2g070780 92.962 341 24 0 6 346 1 341 0.0 646
MS.gene068655.t1 MTR_6g034190 58.269 520 193 4 6 510 1 511 0.0 588
MS.gene068655.t1 MTR_2g072240 50.682 513 180 8 6 510 1 448 4.20e-166 478
MS.gene068655.t1 MTR_2g071700 72.897 321 84 1 22 339 18 338 5.27e-161 461
MS.gene068655.t1 MTR_2g071720 76.243 181 27 1 346 510 1 181 5.54e-94 283
MS.gene068655.t1 MTR_2g070820 89.189 111 12 0 280 390 1 111 7.18e-64 204
MS.gene068655.t1 MTR_2g070660 56.853 197 51 3 6 198 1 167 5.33e-60 195
MS.gene068655.t1 MTR_2g070810 95.745 47 2 0 6 52 49 95 5.58e-23 93.6
MS.gene068655.t1 MTR_2g047858 24.138 464 282 16 8 457 95 502 3.57e-16 81.6
MS.gene068655.t1 MTR_1g016340 22.667 450 287 13 17 457 10 407 2.63e-12 69.3
MS.gene068655.t1 MTR_1g016340 22.667 450 287 13 17 457 15 412 2.82e-12 68.9
MS.gene068655.t1 MTR_8g468470 25.658 456 283 20 21 457 13 431 5.06e-12 68.6
MS.gene068655.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 5.55e-12 68.6
MS.gene068655.t1 MTR_7g095350 27.331 311 193 11 27 328 21 307 6.40e-12 68.6
MS.gene068655.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 6.74e-12 67.8
MS.gene068655.t1 MTR_7g095350 27.331 311 193 11 27 328 13 299 7.05e-12 67.8
MS.gene068655.t1 MTR_7g095350 27.331 311 193 11 27 328 21 307 8.85e-12 67.4
MS.gene068655.t1 MTR_2g082860 56.000 50 22 0 21 70 26 75 1.55e-11 62.0
MS.gene068655.t1 MTR_7g007370 50.820 61 30 0 6 66 1 61 1.86e-11 65.9
MS.gene068655.t1 MTR_8g014180 52.727 55 26 0 6 60 1 55 2.36e-11 59.7
MS.gene068655.t1 MTR_4g024770 40.845 71 42 0 2 72 4 74 2.43e-11 60.1
MS.gene068655.t1 MTR_6g013240 24.242 297 192 10 16 302 66 339 2.82e-11 66.6
MS.gene068655.t1 MTR_6g013380 24.242 297 192 10 16 302 61 334 3.06e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068655.t1 AT1G80960 27.273 462 253 17 22 457 11 415 7.08e-22 98.6
MS.gene068655.t1 AT1G80960 27.273 462 253 17 22 457 50 454 8.01e-22 99.0
MS.gene068655.t1 AT1G80960 27.273 462 253 17 22 457 50 454 8.01e-22 99.0
MS.gene068655.t1 AT1G80960 27.273 462 253 17 22 457 50 454 8.01e-22 99.0
MS.gene068655.t1 AT1G80960 31.023 303 151 11 22 308 50 310 2.29e-21 96.7
MS.gene068655.t1 AT1G80960 31.023 303 151 11 22 308 50 310 2.74e-21 97.1
MS.gene068655.t1 AT3G59190 24.554 505 286 24 15 467 5 466 7.36e-16 80.5
MS.gene068655.t1 AT1G67390 26.923 286 172 8 21 302 40 292 1.07e-15 80.1
MS.gene068655.t1 AT3G26922 27.241 290 159 8 21 302 13 258 6.47e-15 75.9
MS.gene068655.t1 AT3G26922 27.241 290 159 8 21 302 13 258 6.47e-15 75.9
MS.gene068655.t1 AT5G62970 22.845 464 266 17 29 457 10 416 9.49e-13 70.5
MS.gene068655.t1 AT1G78760 22.863 503 284 18 11 481 5 435 1.00e-12 70.5
MS.gene068655.t1 AT5G41830 25.183 409 231 19 11 384 2 370 1.22e-12 70.5
MS.gene068655.t1 AT3G59190 25.310 403 228 18 15 384 5 367 3.66e-12 68.6
MS.gene068655.t1 AT3G49030 25.055 455 257 17 20 457 19 406 6.15e-12 68.2
MS.gene068655.t1 AT3G60790 27.124 306 177 14 7 306 40 305 3.80e-11 65.9

Find 102 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAATCTGTTGAGGATTA+AGG 0.153196 2.2:+21035427 None:intergenic
GTTAAGTACTTAATATTAAA+TGG 0.208632 2.2:-21033793 MS.gene068655:CDS
CCTCACACTCTAGGTCAATT+TGG 0.235008 2.2:-21033962 MS.gene068655:intron
TTCTGAATAATATTACAATT+TGG 0.250934 2.2:-21033765 MS.gene068655:CDS
CTTACCCTGGAAGTTTGTTT+TGG 0.254096 2.2:-21033906 MS.gene068655:CDS
TGTGAACGAGCGTAGGATTT+TGG 0.266869 2.2:-21035244 MS.gene068655:CDS
TGAAATTTGTGGTGGCATTT+TGG 0.276341 2.2:-21035049 MS.gene068655:intron
CCTTGATACAAACATTCTTT+AGG 0.277807 2.2:+21033848 None:intergenic
GATTCTTGTGATTATCGTTA+TGG 0.307940 2.2:-21035567 MS.gene068655:CDS
GCACGTAGAACCCGAAGTTT+TGG 0.308366 2.2:+21035147 None:intergenic
TAAAATGGCTGAAGGAATAA+TGG 0.312589 2.2:-21036374 None:intergenic
TCGTAGCAACTGCATGTTTA+CGG 0.321573 2.2:-21033714 MS.gene068655:CDS
CAAAATCTGTTGAGGATTAA+GGG 0.329839 2.2:+21035428 None:intergenic
TTCAGCCAAAACAAACTTCC+AGG 0.338704 2.2:+21033901 None:intergenic
ATAGAGTATATAAGAAAATA+TGG 0.344916 2.2:-21033643 MS.gene068655:CDS
TTTGTCTTTCGTCAAAATTA+AGG 0.347473 2.2:+21036182 None:intergenic
TACAATAATGTTCAAATGCT+TGG 0.353231 2.2:-21035123 MS.gene068655:CDS
TCTTCCTCCATAACTCTGAT+TGG 0.353522 2.2:+21036334 None:intergenic
AACTTCAATCTTGCAGTGAC+TGG 0.362551 2.2:-21036115 MS.gene068655:CDS
CTTAGAGGCCCCAAAACTTC+GGG 0.365917 2.2:-21035157 MS.gene068655:CDS
TTGTGCAAGTGGGGATGTTG+TGG 0.396215 2.2:-21035643 MS.gene068655:CDS
AGGGATATTATTGAAATTTG+TGG 0.398913 2.2:-21035060 MS.gene068655:CDS
TGTGCAAGTGGGGATGTTGT+GGG 0.402090 2.2:-21035642 MS.gene068655:CDS
CAACTCAAGAACCTTAAAAC+TGG 0.403099 2.2:+21035479 None:intergenic
TTATTTGAAGATTCATTAGT+TGG 0.403722 2.2:+21034073 None:intergenic
TGCAAGATTGAAGTTTAGTC+TGG 0.413386 2.2:+21036123 None:intergenic
TCATGAAGATCTGATCTTAG+AGG 0.415542 2.2:-21035172 MS.gene068655:CDS
TCAAATATCGACAATCATAT+TGG 0.415878 2.2:-21035705 MS.gene068655:CDS
TATTTGAAGATTCATTAGTT+GGG 0.418765 2.2:+21034074 None:intergenic
CCTAAAGAATGTTTGTATCA+AGG 0.437189 2.2:-21033848 MS.gene068655:CDS
AAAATGGCTGAAGGAATAAT+GGG 0.447788 2.2:-21036373 MS.gene068655:CDS
TACAAACATTCTTTAGGTTG+TGG 0.449660 2.2:+21033854 None:intergenic
ATGGTGGTAGCTTTGATGAT+TGG 0.451451 2.2:-21033624 MS.gene068655:CDS
CCACAATGATTCCCATCGCT+TGG 0.455748 2.2:+21036219 None:intergenic
TGGTGGTAGCTTTGATGATT+GGG 0.457946 2.2:-21033623 MS.gene068655:CDS
GTTCTCACGACTTCCGTGTA+AGG 0.459091 2.2:+21036253 None:intergenic
GGGATGTTGTGGGGTGGATG+AGG 0.463671 2.2:-21035632 MS.gene068655:CDS
GCATTCCTTTCAATCATGTC+TGG 0.464346 2.2:+21035537 None:intergenic
TATCCCAGGTTTCAGATCAA+TGG 0.467659 2.2:+21033663 None:intergenic
CTAAAGAATGTTTGTATCAA+GGG 0.467863 2.2:-21033847 MS.gene068655:CDS
TCTAATGCTACAACTCTTCA+AGG 0.468328 2.2:+21035680 None:intergenic
ATATAAGCTAGAAGTGCATC+AGG 0.471803 2.2:+21036289 None:intergenic
TCCCCACTTGCACAACTCTC+AGG 0.473005 2.2:+21035651 None:intergenic
TTATCCATTGATCTGAAACC+TGG 0.478986 2.2:-21033667 MS.gene068655:CDS
AGGGACACCAATCAGAGTTA+TGG 0.491843 2.2:-21036341 MS.gene068655:CDS
ACGACTTCCGTGTAAGGAAG+TGG 0.493916 2.2:+21036259 None:intergenic
AAACTGGAGAAAACTTCAAA+AGG 0.495741 2.2:+21035495 None:intergenic
CATTTCTCCACTTCCTTACA+CGG 0.495992 2.2:-21036266 MS.gene068655:CDS
GTGCAAGTGGGGATGTTGTG+GGG 0.497345 2.2:-21035641 MS.gene068655:CDS
TCTTAGAGGCCCCAAAACTT+CGG 0.498004 2.2:-21035158 MS.gene068655:CDS
GGAATAATGGGTGAAGGAGA+AGG 0.503229 2.2:-21036361 MS.gene068655:CDS
GATAATTTCAGAGAGAGCTT+CGG 0.503369 2.2:+21033685 None:intergenic
CAAGTGGGGATGTTGTGGGG+TGG 0.508240 2.2:-21035638 MS.gene068655:CDS
CAAAACAAACTTCCAGGGTA+AGG 0.518602 2.2:+21033907 None:intergenic
CTTTCGTCAAAATTAAGGCT+AGG 0.519973 2.2:+21036187 None:intergenic
TTAAGAAATCACATTTCTCA+AGG 0.522132 2.2:+21035319 None:intergenic
TCAATTCAAAATCGAGGCAA+AGG 0.529641 2.2:+21034145 None:intergenic
TTTCTTATATACTCTATCCC+AGG 0.537281 2.2:+21033649 None:intergenic
TATCCATTGATCTGAAACCT+GGG 0.540917 2.2:-21033666 MS.gene068655:CDS
ATTTGAAGATTCATTAGTTG+GGG 0.541423 2.2:+21034075 None:intergenic
TGCAGTGACTGGGCAACGTA+AGG 0.547546 2.2:-21036104 MS.gene068655:intron
GAATAATGGGTGAAGGAGAA+GGG 0.553775 2.2:-21036360 MS.gene068655:CDS
CAATTCAAAATCGAGGCAAA+GGG 0.555813 2.2:+21034146 None:intergenic
CAAGTGTGCTCTCCAAGCGA+TGG 0.562082 2.2:-21036231 MS.gene068655:CDS
AACTTCAAAAGGTAAATCAA+TGG 0.562125 2.2:+21035506 None:intergenic
GATATTATTGAAATTTGTGG+TGG 0.564526 2.2:-21035057 MS.gene068655:CDS
TACCGTTCAATTCAAAATCG+AGG 0.564943 2.2:+21034139 None:intergenic
ACTTCAATCTTGCAGTGACT+GGG 0.567133 2.2:-21036114 MS.gene068655:CDS
ATGTGATTTCTTAACAGATG+AGG 0.570169 2.2:-21035310 MS.gene068655:CDS
TCAGCCAAAACAAACTTCCA+GGG 0.571326 2.2:+21033902 None:intergenic
TCTTGTGATTATCGTTATGG+AGG 0.571564 2.2:-21035564 MS.gene068655:CDS
TTTGGTTTCTGCATTTAACA+TGG 0.582670 2.2:-21035226 MS.gene068655:CDS
GAATACCAGACATGATTGAA+AGG 0.585171 2.2:-21035542 MS.gene068655:CDS
AAGTGTGCTCTCCAAGCGAT+GGG 0.588507 2.2:-21036230 MS.gene068655:CDS
TTTGTGATGTGAACGAGCGT+AGG 0.592653 2.2:-21035251 MS.gene068655:CDS
GGTCATCGAGGTTCTAGCAT+GGG 0.596942 2.2:-21034192 MS.gene068655:CDS
GAATCTTCAAATAAATAACC+AGG 0.602091 2.2:-21034064 MS.gene068655:intron
AAAAGGCATAAAATGGCTGA+AGG 0.603046 2.2:-21036382 None:intergenic
TACTATGATCAAAATGACAA+TGG 0.603700 2.2:-21033937 MS.gene068655:CDS
CCAAGCGATGGGAATCATTG+TGG 0.604799 2.2:-21036219 MS.gene068655:CDS
GTTTGTATCAAGGGATACAA+AGG 0.605257 2.2:-21033838 MS.gene068655:CDS
AAGTACTTAATATTAAATGG+AGG 0.612207 2.2:-21033790 MS.gene068655:CDS
ACAGGTAGATCTTGAAAGTG+AGG 0.613723 2.2:-21035760 MS.gene068655:intron
GATATTTGATGTAACCATCA+TGG 0.615010 2.2:+21035719 None:intergenic
TTGTGGCTGACACACCTACA+CGG 0.616160 2.2:+21033871 None:intergenic
AATGGCTGTCTTCCTTACCC+TGG 0.616901 2.2:-21033919 MS.gene068655:CDS
ATCTGTTGAGGATTAAGGGA+GGG 0.619665 2.2:+21035432 None:intergenic
AGAGTATTCAAAATCTGTTG+AGG 0.620364 2.2:+21035420 None:intergenic
TGCCTGAGAGTTGTGCAAGT+GGG 0.620485 2.2:-21035653 MS.gene068655:CDS
GCCTGAGAGTTGTGCAAGTG+GGG 0.621594 2.2:-21035652 MS.gene068655:CDS
CAAGAGATGAACAATGAGAG+AGG 0.622832 2.2:+21035352 None:intergenic
GACACCAATCAGAGTTATGG+AGG 0.626748 2.2:-21036338 MS.gene068655:CDS
GGGTCATCGAGGTTCTAGCA+TGG 0.629061 2.2:-21034193 MS.gene068655:CDS
TGTGGCTGACACACCTACAC+GGG 0.629776 2.2:+21033872 None:intergenic
GCTGAAGGAATAATGGGTGA+AGG 0.630272 2.2:-21036367 MS.gene068655:CDS
CTAATGCTACAACTCTTCAA+GGG 0.636034 2.2:+21035681 None:intergenic
GGCTGAAAAGAGACCCGTGT+AGG 0.671792 2.2:-21033885 MS.gene068655:CDS
TTGCCTGAGAGTTGTGCAAG+TGG 0.675075 2.2:-21035654 MS.gene068655:CDS
GAGTATATAAGAAAATATGG+TGG 0.676614 2.2:-21033640 MS.gene068655:CDS
TGATTGTCTTAGGGTCATCG+AGG 0.687412 2.2:-21034204 MS.gene068655:intron
AATCTGTTGAGGATTAAGGG+AGG 0.688199 2.2:+21035431 None:intergenic
TTTGAAGATTCATTAGTTGG+GGG 0.726871 2.2:+21034076 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATAATTTATATA+TGG - chr2.2:21035157-21035176 MS.gene068655:CDS 0.0%
!! AATTCTTTATATAAATTCTT+TGG + chr2.2:21035670-21035689 None:intergenic 10.0%
!! TCAAATATGATATATATTAT+TGG - chr2.2:21034181-21034200 MS.gene068655:CDS 10.0%
!! ATAGAGTATATAAGAAAATA+TGG - chr2.2:21036333-21036352 MS.gene068655:CDS 15.0%
!! ATGATATCTATTTAATACTT+AGG - chr2.2:21035416-21035435 MS.gene068655:CDS 15.0%
!! GTTAAGTACTTAATATTAAA+TGG - chr2.2:21036183-21036202 MS.gene068655:CDS 15.0%
!! TAGATGAAGTTTAATTATAA+AGG - chr2.2:21034154-21034173 MS.gene068655:CDS 15.0%
!! TTCTGAATAATATTACAATT+TGG - chr2.2:21036211-21036230 MS.gene068655:CDS 15.0%
!!! CACAAATTTTAATATATTTG+AGG + chr2.2:21034711-21034730 None:intergenic 15.0%
!!! CATCTATTTTATTAGAAAAA+TGG + chr2.2:21034140-21034159 None:intergenic 15.0%
!!! TAATATTACAATTTGGTTTT+TGG - chr2.2:21036218-21036237 MS.gene068655:CDS 15.0%
!!! TATATAAAGAATTGAAGTTT+TGG - chr2.2:21035677-21035696 MS.gene068655:CDS 15.0%
!!! TGTTTTTTACTATAAACTTT+AGG - chr2.2:21033929-21033948 MS.gene068655:CDS 15.0%
!! AAATAATAGATACTCATGAT+AGG + chr2.2:21035611-21035630 None:intergenic 20.0%
!! AAGTACTTAATATTAAATGG+AGG - chr2.2:21036186-21036205 MS.gene068655:CDS 20.0%
!! AGTTCATAGCTTATTATATA+TGG - chr2.2:21035351-21035370 MS.gene068655:CDS 20.0%
!! CATTTCTTTATAGTTAATAG+AGG - chr2.2:21035012-21035031 MS.gene068655:intron 20.0%
!! TAATCAATATTTGTAGCATA+AGG + chr2.2:21035503-21035522 None:intergenic 20.0%
!! TATTTGAAGATTCATTAGTT+GGG + chr2.2:21035905-21035924 None:intergenic 20.0%
!! TTATTTGAAGATTCATTAGT+TGG + chr2.2:21035906-21035925 None:intergenic 20.0%
!!! AGGTTTTATTACTATTCAAT+AGG + chr2.2:21035479-21035498 None:intergenic 20.0%
!!! ATTTTAATATATTTGAGGCT+TGG + chr2.2:21034706-21034725 None:intergenic 20.0%
!!! GGTTTTATTACTATTCAATA+GGG + chr2.2:21035478-21035497 None:intergenic 20.0%
!!! TATAGTAATACTACATTTTG+AGG + chr2.2:21035709-21035728 None:intergenic 20.0%
!!! TATATTTTCTGATTGTCTTA+GGG - chr2.2:21035763-21035782 MS.gene068655:intron 20.0%
!!! TTATATTTTCTGATTGTCTT+AGG - chr2.2:21035762-21035781 MS.gene068655:intron 20.0%
! AACTTCAAAAGGTAAATCAA+TGG + chr2.2:21034473-21034492 None:intergenic 25.0%
! ACTAATTGAGTATTTGTGAT+AGG - chr2.2:21035374-21035393 MS.gene068655:CDS 25.0%
! AGGGATATTATTGAAATTTG+TGG - chr2.2:21034916-21034935 MS.gene068655:intron 25.0%
! ATCAAGCAGTTCATATTTAA+TGG - chr2.2:21034065-21034084 MS.gene068655:CDS 25.0%
! ATTTGAAGATTCATTAGTTG+GGG + chr2.2:21035904-21035923 None:intergenic 25.0%
! CTAAAGAATGTTTGTATCAA+GGG - chr2.2:21036129-21036148 MS.gene068655:CDS 25.0%
! GAATCTTCAAATAAATAACC+AGG - chr2.2:21035912-21035931 MS.gene068655:intron 25.0%
! GAGTATATAAGAAAATATGG+TGG - chr2.2:21036336-21036355 MS.gene068655:CDS 25.0%
! GATATTATTGAAATTTGTGG+TGG - chr2.2:21034919-21034938 MS.gene068655:intron 25.0%
! TACAATAATGTTCAAATGCT+TGG - chr2.2:21034853-21034872 MS.gene068655:intron 25.0%
! TACTATGATCAAAATGACAA+TGG - chr2.2:21036039-21036058 MS.gene068655:intron 25.0%
! TATTTAATGGAGAATGAACT+AGG - chr2.2:21034078-21034097 MS.gene068655:CDS 25.0%
! TCAAATATCGACAATCATAT+TGG - chr2.2:21034271-21034290 MS.gene068655:intron 25.0%
! TTAAGAAATCACATTTCTCA+AGG + chr2.2:21034660-21034679 None:intergenic 25.0%
! TTTGTCTTTCGTCAAAATTA+AGG + chr2.2:21033797-21033816 None:intergenic 25.0%
!! TAAGCTATGAACTTTTTGTA+TGG + chr2.2:21035344-21035363 None:intergenic 25.0%
!!! GGACAAAATGTTTTTATAGA+TGG - chr2.2:21034874-21034893 MS.gene068655:intron 25.0%
!!! TTTTTTGTGAAGATTAACCA+TGG + chr2.2:21034019-21034038 None:intergenic 25.0%
AAACTGGAGAAAACTTCAAA+AGG + chr2.2:21034484-21034503 None:intergenic 30.0%
AGCCAAAAAATCAAATTGCA+TGG + chr2.2:21035541-21035560 None:intergenic 30.0%
ATGTGATTTCTTAACAGATG+AGG - chr2.2:21034666-21034685 MS.gene068655:intron 30.0%
CAAAATCTGTTGAGGATTAA+GGG + chr2.2:21034551-21034570 None:intergenic 30.0%
CAATATTTGTAGCATAAGGA+AGG + chr2.2:21035499-21035518 None:intergenic 30.0%
CACAAAAAATCACGCTTATT+GGG - chr2.2:21035311-21035330 MS.gene068655:CDS 30.0%
CCTAAAGAATGTTTGTATCA+AGG - chr2.2:21036128-21036147 MS.gene068655:CDS 30.0%
CCTTGATACAAACATTCTTT+AGG + chr2.2:21036131-21036150 None:intergenic 30.0%
GATATTTGATGTAACCATCA+TGG + chr2.2:21034260-21034279 None:intergenic 30.0%
GATTCTTGTGATTATCGTTA+TGG - chr2.2:21034409-21034428 MS.gene068655:intron 30.0%
TATTGGACTTTATGCATAAC+AGG - chr2.2:21034198-21034217 MS.gene068655:intron 30.0%
TCAAAATCTGTTGAGGATTA+AGG + chr2.2:21034552-21034571 None:intergenic 30.0%
TCACAAAAAATCACGCTTAT+TGG - chr2.2:21035310-21035329 MS.gene068655:CDS 30.0%
TTTCTTATATACTCTATCCC+AGG + chr2.2:21036330-21036349 None:intergenic 30.0%
TTTGAAGATTCATTAGTTGG+GGG + chr2.2:21035903-21035922 None:intergenic 30.0%
! AGAGTATTCAAAATCTGTTG+AGG + chr2.2:21034559-21034578 None:intergenic 30.0%
! TGAAGTTTTCTCCAGTTTTA+AGG - chr2.2:21034486-21034505 MS.gene068655:intron 30.0%
! TTTGGTTTCTGCATTTAACA+TGG - chr2.2:21034750-21034769 MS.gene068655:intron 30.0%
!! GGGAAGTGTCTTTAAAAATT+TGG - chr2.2:21035804-21035823 MS.gene068655:intron 30.0%
!! TACAAACATTCTTTAGGTTG+TGG + chr2.2:21036125-21036144 None:intergenic 30.0%
!!! ATAAGCGTGATTTTTTGTGA+TGG + chr2.2:21035310-21035329 None:intergenic 30.0%
!!! ATTTTTTGTGATGGCTCAAA+AGG + chr2.2:21035301-21035320 None:intergenic 30.0%
!!! CTCCATGCAATTTGATTTTT+TGG - chr2.2:21035536-21035555 MS.gene068655:CDS 30.0%
!!! TTTTTTGTGATGGCTCAAAA+GGG + chr2.2:21035300-21035319 None:intergenic 30.0%
!!! TTTTTTTTGTTGATCGTGTC+TGG - chr2.2:21033971-21033990 MS.gene068655:intron 30.0%
ACTCGAAAAAGAGGTGATTA+AGG - chr2.2:21034372-21034391 MS.gene068655:intron 35.0%
AGGGAATCATACATGCATTA+AGG + chr2.2:21035281-21035300 None:intergenic 35.0%
CAACTCAAGAACCTTAAAAC+TGG + chr2.2:21034500-21034519 None:intergenic 35.0%
CAATTCAAAATCGAGGCAAA+GGG + chr2.2:21035833-21035852 None:intergenic 35.0%
CTAATGCTACAACTCTTCAA+GGG + chr2.2:21034298-21034317 None:intergenic 35.0%
CTTTCGTCAAAATTAAGGCT+AGG + chr2.2:21033792-21033811 None:intergenic 35.0%
GAATACCAGACATGATTGAA+AGG - chr2.2:21034434-21034453 MS.gene068655:intron 35.0%
GAGGAAATTACTCGAAAAAG+AGG - chr2.2:21034363-21034382 MS.gene068655:intron 35.0%
GATAATTTCAGAGAGAGCTT+CGG + chr2.2:21036294-21036313 None:intergenic 35.0%
GTTTGTATCAAGGGATACAA+AGG - chr2.2:21036138-21036157 MS.gene068655:CDS 35.0%
TACCGTTCAATTCAAAATCG+AGG + chr2.2:21035840-21035859 None:intergenic 35.0%
TACTTAGGATGAACACAGAT+TGG - chr2.2:21035431-21035450 MS.gene068655:CDS 35.0%
TCAATTCAAAATCGAGGCAA+AGG + chr2.2:21035834-21035853 None:intergenic 35.0%
TCATGAAGATCTGATCTTAG+AGG - chr2.2:21034804-21034823 MS.gene068655:intron 35.0%
TCTAATGCTACAACTCTTCA+AGG + chr2.2:21034299-21034318 None:intergenic 35.0%
TCTTGTGATTATCGTTATGG+AGG - chr2.2:21034412-21034431 MS.gene068655:intron 35.0%
TGCAAGATTGAAGTTTAGTC+TGG + chr2.2:21033856-21033875 None:intergenic 35.0%
TGCTGCATTACAATTCAACT+AGG - chr2.2:21035062-21035081 MS.gene068655:CDS 35.0%
TTCCAAGTGAAGTATAGTAG+AGG + chr2.2:21034962-21034981 None:intergenic 35.0%
TTCCTCTACTATACTTCACT+TGG - chr2.2:21034957-21034976 MS.gene068655:intron 35.0%
! ATATAAGCTAGAAGTGCATC+AGG + chr2.2:21033690-21033709 None:intergenic 35.0%
! ATGCAAGCTTTTGACAACAA+GGG - chr2.2:21034897-21034916 MS.gene068655:intron 35.0%
! TATCCATTGATCTGAAACCT+GGG - chr2.2:21036310-21036329 MS.gene068655:CDS 35.0%
! TGAAATTTGTGGTGGCATTT+TGG - chr2.2:21034927-21034946 MS.gene068655:intron 35.0%
! TGCCTCGATTTTGAATTGAA+CGG - chr2.2:21035835-21035854 MS.gene068655:intron 35.0%
! TTATCCATTGATCTGAAACC+TGG - chr2.2:21036309-21036328 MS.gene068655:CDS 35.0%
!! ACTATTCAATAGGGTGTTGA+TGG + chr2.2:21035469-21035488 None:intergenic 35.0%
AACTTCAATCTTGCAGTGAC+TGG - chr2.2:21033861-21033880 MS.gene068655:CDS 40.0%
AATCTGTTGAGGATTAAGGG+AGG + chr2.2:21034548-21034567 None:intergenic 40.0%
ACAGGTAGATCTTGAAAGTG+AGG - chr2.2:21034216-21034235 MS.gene068655:intron 40.0%
ACATTTAACCCTCACACTCT+AGG - chr2.2:21036005-21036024 MS.gene068655:intron 40.0%
ACTTCAATCTTGCAGTGACT+GGG - chr2.2:21033862-21033881 MS.gene068655:CDS 40.0%
ATAACCAGGTATGCTTGCTT+AGG - chr2.2:21035926-21035945 MS.gene068655:intron 40.0%
ATCTGTTGAGGATTAAGGGA+GGG + chr2.2:21034547-21034566 None:intergenic 40.0%
CAAAACAAACTTCCAGGGTA+AGG + chr2.2:21036072-21036091 None:intergenic 40.0%
CAAATTGACCTAGAGTGTGA+GGG + chr2.2:21036016-21036035 None:intergenic 40.0%
CAAGAGATGAACAATGAGAG+AGG + chr2.2:21034627-21034646 None:intergenic 40.0%
CATTTCTCCACTTCCTTACA+CGG - chr2.2:21033710-21033729 MS.gene068655:CDS 40.0%
CTATATCTCTAGACAAGCCA+TGG - chr2.2:21033999-21034018 MS.gene068655:intron 40.0%
GCATTCCTTTCAATCATGTC+TGG + chr2.2:21034442-21034461 None:intergenic 40.0%
GGCTTGTCTAGAGATATAGA+GGG + chr2.2:21033998-21034017 None:intergenic 40.0%
TAATCCTAAGCAAGCATACC+TGG + chr2.2:21035933-21035952 None:intergenic 40.0%
TATCCCAGGTTTCAGATCAA+TGG + chr2.2:21036316-21036335 None:intergenic 40.0%
TCAGCCAAAACAAACTTCCA+GGG + chr2.2:21036077-21036096 None:intergenic 40.0%
TCGTAGCAACTGCATGTTTA+CGG - chr2.2:21036262-21036281 MS.gene068655:CDS 40.0%
TCTTCCTCCATAACTCTGAT+TGG + chr2.2:21033645-21033664 None:intergenic 40.0%
TTCAGCCAAAACAAACTTCC+AGG + chr2.2:21036078-21036097 None:intergenic 40.0%
! GATGCAAGCTTTTGACAACA+AGG - chr2.2:21034896-21034915 MS.gene068655:intron 40.0%
! TGGCTTGTCTAGAGATATAG+AGG + chr2.2:21033999-21034018 None:intergenic 40.0%
!! ATGGTGGTAGCTTTGATGAT+TGG - chr2.2:21036352-21036371 MS.gene068655:CDS 40.0%
!! CTTACCCTGGAAGTTTGTTT+TGG - chr2.2:21036070-21036089 MS.gene068655:intron 40.0%
!! GAATAATGGGTGAAGGAGAA+GGG - chr2.2:21033616-21033635 MS.gene068655:CDS 40.0%
!! GTTTTTGGATGACTGCTCAT+GGG - chr2.2:21036233-21036252 MS.gene068655:CDS 40.0%
!! TGGTGGTAGCTTTGATGATT+GGG - chr2.2:21036353-21036372 MS.gene068655:CDS 40.0%
AAGATTCATTAGTTGGGGGC+AGG + chr2.2:21035899-21035918 None:intergenic 45.0%
AGGGACACCAATCAGAGTTA+TGG - chr2.2:21033635-21033654 MS.gene068655:CDS 45.0%
CCAAATTGACCTAGAGTGTG+AGG + chr2.2:21036017-21036036 None:intergenic 45.0%
CCTCACACTCTAGGTCAATT+TGG - chr2.2:21036014-21036033 MS.gene068655:intron 45.0%
GACACCAATCAGAGTTATGG+AGG - chr2.2:21033638-21033657 MS.gene068655:CDS 45.0%
TCTTAGAGGCCCCAAAACTT+CGG - chr2.2:21034818-21034837 MS.gene068655:intron 45.0%
TGATTGTCTTAGGGTCATCG+AGG - chr2.2:21035772-21035791 MS.gene068655:intron 45.0%
TTTGTGATGTGAACGAGCGT+AGG - chr2.2:21034725-21034744 MS.gene068655:intron 45.0%
! ACGTAGAACCCGAAGTTTTG+GGG + chr2.2:21034830-21034849 None:intergenic 45.0%
! CACGTAGAACCCGAAGTTTT+GGG + chr2.2:21034831-21034850 None:intergenic 45.0%
! TGTGAACGAGCGTAGGATTT+TGG - chr2.2:21034732-21034751 MS.gene068655:intron 45.0%
! TTTTGCTGAAGCAGCCATGA+TGG - chr2.2:21034243-21034262 MS.gene068655:intron 45.0%
!! GCTGAAGGAATAATGGGTGA+AGG - chr2.2:21033609-21033628 MS.gene068655:CDS 45.0%
!! GGAATAATGGGTGAAGGAGA+AGG - chr2.2:21033615-21033634 MS.gene068655:CDS 45.0%
!! GGTTTTTGGATGACTGCTCA+TGG - chr2.2:21036232-21036251 MS.gene068655:CDS 45.0%
AAGTGTGCTCTCCAAGCGAT+GGG - chr2.2:21033746-21033765 MS.gene068655:CDS 50.0%
AATGGCTGTCTTCCTTACCC+TGG - chr2.2:21036057-21036076 MS.gene068655:intron 50.0%
ACGACTTCCGTGTAAGGAAG+TGG + chr2.2:21033720-21033739 None:intergenic 50.0%
CCAAGCGATGGGAATCATTG+TGG - chr2.2:21033757-21033776 MS.gene068655:CDS 50.0%
CCACAATGATTCCCATCGCT+TGG + chr2.2:21033760-21033779 None:intergenic 50.0%
CTTAGAGGCCCCAAAACTTC+GGG - chr2.2:21034819-21034838 MS.gene068655:intron 50.0%
GTTCTCACGACTTCCGTGTA+AGG + chr2.2:21033726-21033745 None:intergenic 50.0%
TGCCTGAGAGTTGTGCAAGT+GGG - chr2.2:21034323-21034342 MS.gene068655:intron 50.0%
TGTGCAAGTGGGGATGTTGT+GGG - chr2.2:21034334-21034353 MS.gene068655:intron 50.0%
TTGCCTGAGAGTTGTGCAAG+TGG - chr2.2:21034322-21034341 MS.gene068655:intron 50.0%
TTGTGCAAGTGGGGATGTTG+TGG - chr2.2:21034333-21034352 MS.gene068655:intron 50.0%
TTGTGGCTGACACACCTACA+CGG + chr2.2:21036108-21036127 None:intergenic 50.0%
! GCACGTAGAACCCGAAGTTT+TGG + chr2.2:21034832-21034851 None:intergenic 50.0%
! GGTCATCGAGGTTCTAGCAT+GGG - chr2.2:21035784-21035803 MS.gene068655:intron 50.0%
!! TAGGGTGTTGATGGCGTACT+AGG + chr2.2:21035460-21035479 None:intergenic 50.0%
CAAGTGTGCTCTCCAAGCGA+TGG - chr2.2:21033745-21033764 MS.gene068655:CDS 55.0%
GCCTGAGAGTTGTGCAAGTG+GGG - chr2.2:21034324-21034343 MS.gene068655:intron 55.0%
GGCTGAAAAGAGACCCGTGT+AGG - chr2.2:21036091-21036110 MS.gene068655:intron 55.0%
GTGCAAGTGGGGATGTTGTG+GGG - chr2.2:21034335-21034354 MS.gene068655:intron 55.0%
TCCCCACTTGCACAACTCTC+AGG + chr2.2:21034328-21034347 None:intergenic 55.0%
TGCAGTGACTGGGCAACGTA+AGG - chr2.2:21033872-21033891 MS.gene068655:CDS 55.0%
TGTGGCTGACACACCTACAC+GGG + chr2.2:21036107-21036126 None:intergenic 55.0%
! GGGTCATCGAGGTTCTAGCA+TGG - chr2.2:21035783-21035802 MS.gene068655:intron 55.0%
CAAGTGGGGATGTTGTGGGG+TGG - chr2.2:21034338-21034357 MS.gene068655:intron 60.0%
GGGATGTTGTGGGGTGGATG+AGG - chr2.2:21034344-21034363 MS.gene068655:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 21033606 21036392 21033606 ID=MS.gene068655
chr2.2 mRNA 21033606 21036392 21033606 ID=MS.gene068655.t1;Parent=MS.gene068655
chr2.2 exon 21036105 21036392 21036105 ID=MS.gene068655.t1.exon1;Parent=MS.gene068655.t1
chr2.2 CDS 21036105 21036392 21036105 ID=cds.MS.gene068655.t1;Parent=MS.gene068655.t1
chr2.2 exon 21035050 21035778 21035050 ID=MS.gene068655.t1.exon2;Parent=MS.gene068655.t1
chr2.2 CDS 21035050 21035778 21035050 ID=cds.MS.gene068655.t1;Parent=MS.gene068655.t1
chr2.2 exon 21034065 21034214 21034065 ID=MS.gene068655.t1.exon3;Parent=MS.gene068655.t1
chr2.2 CDS 21034065 21034214 21034065 ID=cds.MS.gene068655.t1;Parent=MS.gene068655.t1
chr2.2 exon 21033606 21033971 21033606 ID=MS.gene068655.t1.exon4;Parent=MS.gene068655.t1
chr2.2 CDS 21033606 21033971 21033606 ID=cds.MS.gene068655.t1;Parent=MS.gene068655.t1
Gene Sequence

>MS.gene068655

ATGGCTGAAGGAATAATGGGTGAAGGAGAAGGGACACCAATCAGAGTTATGGAGGAAGAAGAAGATTTGATCAGCAATCTTCCTGATGCACTTCTAGCTTATATCATTTCTCCACTTCCTTACACGGAAGTCGTGAGAACAAGTGTGCTCTCCAAGCGATGGGAATCATTGTGGAAACACACTCCTAGCCTTAATTTTGACGAAAGACAAATGTTAAAGTCATTGATCAAAGAAAACAATCAAAATTCCAGACTAAACTTCAATCTTGCAGTGACTGGGCAACGTAAGGTACATTGTTATAAAAATTTATGTAAAATTTTATGTGTTTTTTACTATAAACTTTAGGTTTTAATTTATGATTGTTTTTTTTTTTGTTGATCGTGTCTGGTCCCTCTATATCTCTAGACAAGCCATGGTTAATCTTCACAAAAAATTATAGATATCTTAATAATTAGATTAATCAAGCAGTTCATATTTAATGGAGAATGAACTAGGAAAAATTAGAGAGAATATGATTACATATTTTAGCTTCCATTTTTCTAATAAAATAGATGAAGTTTAATTATAAAGGCTTTTCAAATATGATATATATTATTGGACTTTATGCATAACAGGTAGATCTTGAAAGTGAGGAACTTTTTGCTGAAGCAGCCATGATGGTTACATCAAATATCGACAATCATATTGGTCCCTTGAAGAGTTGTAGCATTAGACACTTGCCTGAGAGTTGTGCAAGTGGGGATGTTGTGGGGTGGATGAGGAAATTACTCGAAAAAGAGGTGATTAAGGTTTCAATAGAACGTGATTCTTGTGATTATCGTTATGGAGGAATACCAGACATGATTGAAAGGAATGCTGCATCAACCATTGATTTACCTTTTGAAGTTTTCTCCAGTTTTAAGGTTCTTGAGTTGAAGAACTATCATTTTTTAACTACACCCTCCCTTAATCCTCAACAGATTTTGAATACTCTTACTCTTAACAAAGTGCATATTATATCTAACACTTTTCATGATATCCTCTCTCATTGTTCATCTCTTGAAAATCTTATCCTTGAGAAATGTGATTTCTTAACAGATGAGGTTAAGATTGTAAGTCCAAGCCTCAAATATATTAAAATTTGTGATGTGAACGAGCGTAGGATTTTGGTTTCTGCATTTAACATGGAAGTCATAGAGATTGACTCTGTTACTTGTAATCATGAAGATCTGATCTTAGAGGCCCCAAAACTTCGGGTTCTACGTGCTTACAATAATGTTCAAATGCTTGGACAAAATGTTTTTATAGATGGATGCAAGCTTTTGACAACAAGGGATATTATTGAAATTTGTGGTGGCATTTTGGTAAGTGTTTCCTCTACTATACTTCACTTGGAATGTCAGTTTTTTTGATTGATGCATATATTACATTTCTTTATAGTTAATAGAGGTGCATGTAATCTAATGTTTTAAATTTTTGCTGCATTACAATTCAACTAGGATATATATGTGTGTGTGTCTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAATTTATATATGGTTCATATTCAATTACAATTGAATATTACAATCATATGTACATAGATGCAATCATTAAGATTAATACGTGAGTCAATGATAATGATTAAACTTTAATATCCTTAATGCATGTATGATTCCCTTTTGAGCCATCACAAAAAATCACGCTTATTGGGCAATACTCCATACAAAAAGTTCATAGCTTATTATATATGGACTAATTGAGTATTTGTGATAGGATAATAAAACTTGTTGTTTATGATATCTATTTAATACTTAGGATGAACACAGATTGGTAACCTAGTACGCCATCAACACCCTATTGAATAGTAATAAAACCTTCCTTATGCTACAAATATTGATTAGAGTTAGAAAACTCTCCATGCAATTTGATTTTTTGGCTTAGAAATATGTCATGTAAAAAATAGACAATTATATTTTAGTTTTTGACCTATCATGAGTATCTATTATTTAGATCGTTCAGTAATGCTAATTAAATTTTACAGCAACCAAAGAATTTATATAAAGAATTGAAGTTTTGGTTGTATCCTCAAAATGTAGTATTACTATAATTTATGAACATTTATGTCTATTTGTTTTATAATTATATTTTCTGATTGTCTTAGGGTCATCGAGGTTCTAGCATGGGAAGTGTCTTTAAAAATTTGGTTACCCTTTGCCTCGATTTTGAATTGAACGGTAATAGAAATGTCATAGCTCTTTCTTTTGCACTCAAGTCCTGCCCCCAACTAATGAATCTTCAAATAAATAACCAGGTATGCTTGCTTAGGATTATTTTAGCTTGATCATGATTGCAGTCATTATACTTAACTATATATTTTTTTTGACATTTAACCCTCACACTCTAGGTCAATTTGGATTACTATGATCAAAATGACAATGGCTGTCTTCCTTACCCTGGAAGTTTGTTTTGGCTGAAAAGAGACCCGTGTAGGTGTGTCAGCCACAACCTAAAGAATGTTTGTATCAAGGGATACAAAGGTGAAGAGTTTGAAATTGAATTTGTTAAGTACTTAATATTAAATGGAGGAGTTCTGAATAATATTACAATTTGGTTTTTGGATGACTGCTCATGGGATGACGTCGTAGCAACTGCATGTTTACGGTCATATCCGAAGCTCTCTCTGAAATTATCCATTGATCTGAAACCTGGGATAGAGTATATAAGAAAATATGGTGGTAGCTTTGATGATTGGGTCCGAACCCTAAAATAA
Protein sequence

>MS.gene068655.t1

MAEGIMGEGEGTPIRVMEEEEDLISNLPDALLAYIISPLPYTEVVRTSVLSKRWESLWKHTPSLNFDERQMLKSLIKENNQNSRLNFNLAVTGQRKVDLESEELFAEAAMMVTSNIDNHIGPLKSCSIRHLPESCASGDVVGWMRKLLEKEVIKVSIERDSCDYRYGGIPDMIERNAASTIDLPFEVFSSFKVLELKNYHFLTTPSLNPQQILNTLTLNKVHIISNTFHDILSHCSSLENLILEKCDFLTDEVKIVSPSLKYIKICDVNERRILVSAFNMEVIEIDSVTCNHEDLILEAPKLRVLRAYNNVQMLGQNVFIDGCKLLTTRDIIEICGGILGHRGSSMGSVFKNLVTLCLDFELNGNRNVIALSFALKSCPQLMNLQINNQVNLDYYDQNDNGCLPYPGSLFWLKRDPCRCVSHNLKNVCIKGYKGEEFEIEFVKYLILNGGVLNNITIWFLDDCSWDDVVATACLRSYPKLSLKLSIDLKPGIEYIRKYGGSFDDWVRTLK