AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068656

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068656.t1 MTR_2g070780 89.150 341 23 1 6 332 1 341 0.0 614
MS.gene068656.t1 MTR_6g034190 57.611 519 184 4 6 496 1 511 0.0 567
MS.gene068656.t1 MTR_2g072240 50.784 510 170 9 6 496 1 448 5.73e-161 464
MS.gene068656.t1 MTR_2g071700 68.047 338 90 3 6 325 1 338 6.59e-148 427
MS.gene068656.t1 MTR_2g071720 78.453 181 23 1 332 496 1 181 4.47e-96 288
MS.gene068656.t1 MTR_2g070820 89.189 111 12 0 266 376 1 111 1.02e-63 203
MS.gene068656.t1 MTR_2g070660 56.725 171 40 3 6 172 1 141 1.98e-49 167
MS.gene068656.t1 MTR_2g070810 93.617 47 3 0 6 52 49 95 4.01e-23 94.0
MS.gene068656.t1 MTR_2g047858 24.424 434 278 14 18 443 111 502 9.79e-16 80.1
MS.gene068656.t1 MTR_8g014180 56.364 55 24 0 6 60 1 55 1.16e-12 63.2
MS.gene068656.t1 MTR_4g024770 40.845 71 42 0 2 72 4 74 7.11e-12 61.6
MS.gene068656.t1 MTR_8g468470 24.888 446 285 18 21 443 13 431 1.30e-11 67.0
MS.gene068656.t1 MTR_7g007370 49.180 61 31 0 6 66 1 61 2.11e-11 65.9
MS.gene068656.t1 MTR_1g016310 28.137 263 160 9 16 273 14 252 4.39e-11 65.1
MS.gene068656.t1 MTR_2g082860 54.000 50 23 0 21 70 26 75 5.96e-11 60.5
MS.gene068656.t1 MTR_1g016310 28.137 263 160 9 16 273 14 252 7.47e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068656.t1 AT1G80960 30.314 287 160 11 22 294 11 271 6.33e-21 95.9
MS.gene068656.t1 AT1G80960 30.314 287 160 11 22 294 50 310 9.71e-21 95.1
MS.gene068656.t1 AT1G80960 30.314 287 160 11 22 294 50 310 1.13e-20 95.1
MS.gene068656.t1 AT1G80960 30.314 287 160 11 22 294 50 310 1.13e-20 95.1
MS.gene068656.t1 AT1G80960 30.314 287 160 11 22 294 50 310 1.13e-20 95.1
MS.gene068656.t1 AT1G80960 30.314 287 160 11 22 294 50 310 1.14e-20 94.4
MS.gene068656.t1 AT1G69630 24.440 491 267 19 19 467 15 443 9.42e-17 83.2
MS.gene068656.t1 AT1G67390 26.117 291 181 8 7 288 27 292 2.12e-15 79.0
MS.gene068656.t1 AT3G26922 28.000 275 162 7 21 288 13 258 2.60e-15 77.0
MS.gene068656.t1 AT3G26922 28.000 275 162 7 21 288 13 258 2.60e-15 77.0
MS.gene068656.t1 AT3G59190 24.085 519 290 25 21 474 11 490 5.19e-15 77.8
MS.gene068656.t1 AT5G22730 22.316 475 274 19 9 443 15 434 1.63e-14 76.3
MS.gene068656.t1 AT5G22730 22.316 475 274 19 9 443 15 434 1.63e-14 76.3
MS.gene068656.t1 AT1G78760 23.191 470 261 18 11 443 5 411 2.90e-13 72.0
MS.gene068656.t1 AT3G58860 23.434 431 276 13 19 432 4 397 3.65e-13 72.0
MS.gene068656.t1 AT3G59190 25.000 392 217 17 21 370 11 367 5.01e-12 68.2
MS.gene068656.t1 AT4G00315 23.077 468 257 18 22 445 2 410 1.23e-11 67.0
MS.gene068656.t1 AT3G60790 27.124 306 163 15 7 292 40 305 2.29e-11 66.2
MS.gene068656.t1 AT5G62970 21.111 450 277 13 29 443 10 416 3.70e-11 65.5
MS.gene068656.t1 AT5G41830 23.926 489 283 25 11 455 2 445 7.18e-11 65.1
MS.gene068656.t1 AT3G59170 26.351 296 179 11 21 285 6 293 9.00e-11 64.3

Find 107 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAATCTTAATATATTTA+AGG 0.083479 2.2:+21050251 None:intergenic
TCAAAATCTGTTGGGGATTA+AGG 0.123792 2.2:+21050410 None:intergenic
GTTAAGTACTTAATATTAAA+TGG 0.208632 2.2:-21048776 MS.gene068656:CDS
CCTCACCCTCTAGGTCAATT+TGG 0.227733 2.2:-21048945 MS.gene068656:intron
CAAAATGTCTGAAGAAATAA+TGG 0.265099 2.2:-21051419 None:intergenic
TGTGAACGAGCGTAGGATTT+TGG 0.266869 2.2:-21050227 MS.gene068656:CDS
CCTAAAGAATGTTCGTATAA+AGG 0.268472 2.2:-21048831 MS.gene068656:CDS
TGAAATTTGTGGTGGCATTT+TGG 0.276341 2.2:-21050032 MS.gene068656:intron
CCTTTATACGAACATTCTTT+AGG 0.280859 2.2:+21048831 None:intergenic
CTTACCCCGGAGGTTTGTTT+TGG 0.286540 2.2:-21048889 MS.gene068656:CDS
TTATGAATAATATTACAATT+TGG 0.298100 2.2:-21048748 MS.gene068656:CDS
GCACGTAGAACCCGAAGTTT+TGG 0.308366 2.2:+21050130 None:intergenic
CAAAATCTGTTGGGGATTAA+GGG 0.328803 2.2:+21050411 None:intergenic
ACGACTTTAGCATTTGTCTT+TGG 0.333956 2.2:+21051215 None:intergenic
TGAATCTTCAAATAAATAAC+CGG 0.337383 2.2:-21049110 MS.gene068656:CDS
CTAAAGAATGTTCGTATAAA+GGG 0.341817 2.2:-21048830 MS.gene068656:CDS
ATAGAGTATATAAGAAAATA+TGG 0.344916 2.2:-21048626 MS.gene068656:CDS
TTCAGATCAATGGATAATTT+CGG 0.351106 2.2:+21048656 None:intergenic
TTTGTCTTTGGTCAAAATTA+AGG 0.352740 2.2:+21051227 None:intergenic
TACAATAATGTTCAAATGCT+TGG 0.353231 2.2:-21050106 MS.gene068656:CDS
AACTTCTACCTTGCAGTGAC+TGG 0.364847 2.2:-21051160 MS.gene068656:CDS
CTTAGAGGCCCCAAAACTTC+GGG 0.365917 2.2:-21050140 MS.gene068656:CDS
TTGTGCAAGTGGGGATGTTG+TGG 0.396215 2.2:-21050625 MS.gene068656:CDS
ATCTTAATATATTTAAGGCT+TGG 0.397703 2.2:+21050256 None:intergenic
AGGGATATTATTGAAATTTG+TGG 0.398913 2.2:-21050043 MS.gene068656:CDS
TGTGCAAGTGGGGATGTTGT+GGG 0.402090 2.2:-21050624 MS.gene068656:CDS
TTATTTGAAGATTCATTAGT+TGG 0.403722 2.2:+21049118 None:intergenic
TCATGAAGATCTGATCTTAG+AGG 0.415542 2.2:-21050155 MS.gene068656:CDS
TATTTGAAGATTCATTAGTT+GGG 0.418765 2.2:+21049119 None:intergenic
TCAAATATTGACAATCATAT+TGG 0.420752 2.2:-21050687 MS.gene068656:CDS
GAGAGTATTCAAAATCTGTT+GGG 0.424914 2.2:+21050402 None:intergenic
CGCATCCTTTCAATCAAGTC+TGG 0.429400 2.2:+21050519 None:intergenic
GGGATGTTGTGGGGTGGATG+AGG 0.442773 2.2:-21050614 MS.gene068656:CDS
TACGAACATTCTTTAGGTTG+TGG 0.447306 2.2:+21048837 None:intergenic
TTTCAGCCAAAACAAACCTC+CGG 0.449764 2.2:+21048883 None:intergenic
TGAGAACAAGTGTGCTCTCC+AGG 0.452406 2.2:-21051282 MS.gene068656:CDS
GTTCTCACGACTTCCGTGTA+AGG 0.459091 2.2:+21051298 None:intergenic
CCACAATGATTCCCATCGCC+TGG 0.464696 2.2:+21051264 None:intergenic
TATCCCAGGTTTCAGATCAA+TGG 0.467659 2.2:+21048646 None:intergenic
TCTAATGCTACAACTCTTCA+AGG 0.468328 2.2:+21050662 None:intergenic
ATATAAGCTAGAAGTGCATC+AGG 0.471803 2.2:+21051334 None:intergenic
TCCCCACTTGCACAACTCTC+AGG 0.473005 2.2:+21050633 None:intergenic
CAAAACAAACCTCCGGGGTA+AGG 0.473251 2.2:+21048890 None:intergenic
TGCAAGGTAGAAGTTTAGTC+TGG 0.473542 2.2:+21051168 None:intergenic
TTATCCATTGATCTGAAACC+TGG 0.478986 2.2:-21048650 MS.gene068656:CDS
AGGGACACAAATCAAAGTTA+TGG 0.481343 2.2:-21051386 MS.gene068656:CDS
TTCAGCCAAAACAAACCTCC+GGG 0.483549 2.2:+21048884 None:intergenic
ACGACTTCCGTGTAAGGAAG+TGG 0.493916 2.2:+21051304 None:intergenic
CATTTCTCCACTTCCTTACA+CGG 0.495992 2.2:-21051311 MS.gene068656:CDS
GATTCATGTGATTATCGTTA+CGG 0.496220 2.2:-21050549 MS.gene068656:CDS
GTGCAAGTGGGGATGTTGTG+GGG 0.497345 2.2:-21050623 MS.gene068656:CDS
TCTTAGAGGCCCCAAAACTT+CGG 0.498004 2.2:-21050141 MS.gene068656:CDS
ATCTTCATGATTACAAATAA+CGG 0.505209 2.2:+21050168 None:intergenic
TCAATTCAAAATCGAGGCAA+AGG 0.505524 2.2:+21049190 None:intergenic
TGAGAGTATTCAAAATCTGT+TGG 0.507606 2.2:+21050401 None:intergenic
CAAGTGGGGATGTTGTGGGG+TGG 0.508240 2.2:-21050620 MS.gene068656:CDS
GAAATAATGGGTGAAGGAGA+AGG 0.509757 2.2:-21051406 MS.gene068656:CDS
CAAGTGTGCTCTCCAGGCGA+TGG 0.510249 2.2:-21051276 MS.gene068656:CDS
CTTTGGTCAAAATTAAGGCT+AGG 0.519973 2.2:+21051232 None:intergenic
AAAATGTCTGAAGAAATAAT+GGG 0.529206 2.2:-21051418 MS.gene068656:CDS
TTACGAAATCACATTTCTCA+AGG 0.534571 2.2:+21050302 None:intergenic
TTTCTTATATACTCTATCCC+AGG 0.537281 2.2:+21048632 None:intergenic
TATCCATTGATCTGAAACCT+GGG 0.540917 2.2:-21048649 MS.gene068656:CDS
ATTTGAAGATTCATTAGTTG+GGG 0.541423 2.2:+21049120 None:intergenic
TACCATTCAATTCAAAATCG+AGG 0.544045 2.2:+21049184 None:intergenic
AAATAATGGGTGAAGGAGAA+GGG 0.545422 2.2:-21051405 MS.gene068656:CDS
TGCAGTGACTGGGCAACGTA+AGG 0.547546 2.2:-21051149 MS.gene068656:intron
GATATTATTGAAATTTGTGG+TGG 0.564526 2.2:-21050040 MS.gene068656:CDS
CAATTCAAAATCGAGGCAAA+GGG 0.566625 2.2:+21049191 None:intergenic
ACTTCTACCTTGCAGTGACT+GGG 0.567012 2.2:-21051159 MS.gene068656:CDS
AAGTGTGCTCTCCAGGCGAT+GGG 0.567840 2.2:-21051275 MS.gene068656:CDS
TTTGGTTTCTGCATTTAACA+TGG 0.582670 2.2:-21050209 MS.gene068656:CDS
GAATACCAGACTTGATTGAA+AGG 0.587306 2.2:-21050524 MS.gene068656:CDS
TCTGAAGAAATAATGGGTGA+AGG 0.588283 2.2:-21051412 MS.gene068656:CDS
GGTCATCGAGGTTCTAGCAT+GGG 0.596942 2.2:-21049237 MS.gene068656:CDS
GTTCGTATAAAGGGATACAA+AGG 0.597350 2.2:-21048821 MS.gene068656:CDS
GACACAAATCAAAGTTATGG+AGG 0.600709 2.2:-21051383 MS.gene068656:CDS
TGGTGGTAGCTTTGATGAGT+GGG 0.600866 2.2:-21048606 MS.gene068656:CDS
TTTGTGATGTGAACGAGCGT+AGG 0.601006 2.2:-21050234 MS.gene068656:CDS
AATATTTGATGTAATCATCA+TGG 0.606995 2.2:+21050701 None:intergenic
TACGTTGCCCAGTCACTGCA+AGG 0.609512 2.2:+21051152 None:intergenic
CCAGGCGATGGGAATCATTG+TGG 0.611060 2.2:-21051264 MS.gene068656:CDS
ATGTGATTTCGTAACAGATG+AGG 0.611173 2.2:-21050293 MS.gene068656:CDS
GAGTGCAAAAGAAAGAGCTA+TGG 0.611287 2.2:+21049152 None:intergenic
AAGTACTTAATATTAAATGG+AGG 0.612207 2.2:-21048773 MS.gene068656:CDS
GAATCTTCAAATAAATAACC+GGG 0.615702 2.2:-21049109 MS.gene068656:intron
TTGTGGCTGACACACCTACA+CGG 0.616160 2.2:+21048854 None:intergenic
ATCTGTTGGGGATTAAGGGA+GGG 0.617258 2.2:+21050415 None:intergenic
TGCCTGAGAGTTGTGCAAGT+GGG 0.620485 2.2:-21050635 MS.gene068656:CDS
GCCTGAGAGTTGTGCAAGTG+GGG 0.621594 2.2:-21050634 MS.gene068656:CDS
ACAGGTAGATCTTGAAAGTG+AGG 0.625502 2.2:-21050742 MS.gene068656:intron
CGTATAAAGGGATACAAAGG+TGG 0.628295 2.2:-21048818 MS.gene068656:CDS
GGGTCATCGAGGTTCTAGCA+TGG 0.629061 2.2:-21049238 MS.gene068656:CDS
TGTGGCTGACACACCTACAC+GGG 0.629776 2.2:+21048855 None:intergenic
CTAATGCTACAACTCTTCAA+GGG 0.636034 2.2:+21050663 None:intergenic
GACTGTCTTCCTTACCCCGG+AGG 0.641992 2.2:-21048899 MS.gene068656:CDS
TCATGTGATTATCGTTACGG+AGG 0.650111 2.2:-21050546 MS.gene068656:CDS
AGAGTATTCAAAATCTGTTG+GGG 0.653579 2.2:+21050403 None:intergenic
AATCTGTTGGGGATTAAGGG+AGG 0.657486 2.2:+21050414 None:intergenic
GGCTGAAAAGAGACCCGTGT+AGG 0.671792 2.2:-21048868 MS.gene068656:CDS
ATGGTGGTAGCTTTGATGAG+TGG 0.672519 2.2:-21048607 MS.gene068656:CDS
TTGCCTGAGAGTTGTGCAAG+TGG 0.675075 2.2:-21050636 MS.gene068656:CDS
GAGTATATAAGAAAATATGG+TGG 0.676614 2.2:-21048623 MS.gene068656:CDS
AATGACTGTCTTCCTTACCC+CGG 0.683541 2.2:-21048902 MS.gene068656:CDS
TGATTGTCTTAGGGTCATCG+AGG 0.689894 2.2:-21049249 MS.gene068656:intron
TTTGAAGATTCATTAGTTGG+GGG 0.726871 2.2:+21049121 None:intergenic
TCAGCCAAAACAAACCTCCG+GGG 0.730392 2.2:+21048885 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATAAAATTTAATATA+TGG - chr2.2:21050140-21050159 MS.gene068656:CDS 0.0%
!! TATATATATATATATATTAT+TGG - chr2.2:21049227-21049246 MS.gene068656:CDS 0.0%
!! TTATGAATAATATTACAATT+TGG - chr2.2:21051256-21051275 MS.gene068656:CDS 10.0%
!! ATAGAGTATATAAGAAAATA+TGG - chr2.2:21051378-21051397 MS.gene068656:CDS 15.0%
!! CAATAATTAGATTAATCAAA+CGG - chr2.2:21049039-21049058 MS.gene068656:intron 15.0%
!! CACAAATCTTAATATATTTA+AGG + chr2.2:21049756-21049775 None:intergenic 15.0%
!! GTTAAGTACTTAATATTAAA+TGG - chr2.2:21051228-21051247 MS.gene068656:CDS 15.0%
!!! ATAATAAAACTTGTTGTTTA+TGG - chr2.2:21050378-21050397 MS.gene068656:CDS 15.0%
!!! CATCTATTTTATTAGAAAAA+TGG + chr2.2:21049128-21049147 None:intergenic 15.0%
!!! TAATATTACAATTTGGTTTT+TGG - chr2.2:21051263-21051282 MS.gene068656:CDS 15.0%
!!! TATATTTTATGATTGTCTTA+GGG - chr2.2:21050746-21050765 MS.gene068656:intron 15.0%
!!! TGTTTTTTACTATAAACTTT+AGG - chr2.2:21048914-21048933 MS.gene068656:CDS 15.0%
!!! TTATATTTTATGATTGTCTT+AGG - chr2.2:21050745-21050764 MS.gene068656:intron 15.0%
!! AAATTAATTATAAGCATACC+CGG + chr2.2:21050916-21050935 None:intergenic 20.0%
!! AAGTACTTAATATTAAATGG+AGG - chr2.2:21051231-21051250 MS.gene068656:CDS 20.0%
!! AATATTTGATGTAATCATCA+TGG + chr2.2:21049306-21049325 None:intergenic 20.0%
!! ATCTTCATGATTACAAATAA+CGG + chr2.2:21049839-21049858 None:intergenic 20.0%
!! TATTTGAAGATTCATTAGTT+GGG + chr2.2:21050888-21050907 None:intergenic 20.0%
!! TCAAATATTGACAATCATAT+TGG - chr2.2:21049317-21049336 MS.gene068656:intron 20.0%
!! TGAATCTTCAAATAAATAAC+CGG - chr2.2:21050894-21050913 MS.gene068656:intron 20.0%
!! TTATTTGAAGATTCATTAGT+TGG + chr2.2:21050889-21050908 None:intergenic 20.0%
!!! AATGTCAATTTTTTGATTGA+TGG - chr2.2:21050035-21050054 MS.gene068656:CDS 20.0%
!!! ATCTTAATATATTTAAGGCT+TGG + chr2.2:21049751-21049770 None:intergenic 20.0%
!!! ATGTCAATTTTTTGATTGAT+GGG - chr2.2:21050036-21050055 MS.gene068656:CDS 20.0%
!!! GGTTTTATTACTATTCAATA+TGG + chr2.2:21050461-21050480 None:intergenic 20.0%
!!! TAAGCTATGAACTTTTTATA+TGG + chr2.2:21050325-21050344 None:intergenic 20.0%
!!! TTTTTGTGAAGATTATCTAA+TGG + chr2.2:21049006-21049025 None:intergenic 20.0%
! AAACAATAGATACTCATGAT+AGG + chr2.2:21050594-21050613 None:intergenic 25.0%
! ACTAATTGAGTAATTGTGAT+AGG - chr2.2:21050355-21050374 MS.gene068656:CDS 25.0%
! AGGGATATTATTGAAATTTG+TGG - chr2.2:21049961-21049980 MS.gene068656:intron 25.0%
! AGTTCATAGCTTATTATATG+TGG - chr2.2:21050332-21050351 MS.gene068656:CDS 25.0%
! ATCATATTTGAAAAGCCTTT+AGG + chr2.2:21049160-21049179 None:intergenic 25.0%
! ATGCAAACATTAAGATTAAC+AGG - chr2.2:21050205-21050224 MS.gene068656:CDS 25.0%
! ATGGTATCTATTTAATGCTT+AGG - chr2.2:21050397-21050416 MS.gene068656:CDS 25.0%
! ATTTGAAGATTCATTAGTTG+GGG + chr2.2:21050887-21050906 None:intergenic 25.0%
! CTAAAGAATGTTCGTATAAA+GGG - chr2.2:21051174-21051193 MS.gene068656:CDS 25.0%
! GAATCTTCAAATAAATAACC+GGG - chr2.2:21050895-21050914 MS.gene068656:intron 25.0%
! GAGTATATAAGAAAATATGG+TGG - chr2.2:21051381-21051400 MS.gene068656:CDS 25.0%
! GATATTATTGAAATTTGTGG+TGG - chr2.2:21049964-21049983 MS.gene068656:intron 25.0%
! GGTTCATATTCAATTACAAT+TGG - chr2.2:21050161-21050180 MS.gene068656:CDS 25.0%
! TAATCAATATTTGTAGCATG+AGG + chr2.2:21050486-21050505 None:intergenic 25.0%
! TACAATAATGTTCAAATGCT+TGG - chr2.2:21049898-21049917 MS.gene068656:intron 25.0%
! TAGATGAACTTTAATCCTAA+AGG - chr2.2:21049142-21049161 MS.gene068656:CDS 25.0%
! TCACAAAAAATCACACTTAT+TGG - chr2.2:21050291-21050310 MS.gene068656:CDS 25.0%
! TTCAGATCAATGGATAATTT+CGG + chr2.2:21051351-21051370 None:intergenic 25.0%
! TTTGTCTTTGGTCAAAATTA+AGG + chr2.2:21048780-21048799 None:intergenic 25.0%
!!! ATAAGTGTGATTTTTTGTGA+TGG + chr2.2:21050291-21050310 None:intergenic 25.0%
!!! CATTTCTTTCTAGTTAATAG+AGG - chr2.2:21050066-21050085 MS.gene068656:CDS 25.0%
!!! GGACAAAATGTTTTTATAGA+TGG - chr2.2:21049919-21049938 MS.gene068656:intron 25.0%
!!! GGGAAGTATCTTTAAAAATT+TGG - chr2.2:21050787-21050806 MS.gene068656:intron 25.0%
AACCTCAAAAGGTAAATCAA+TGG + chr2.2:21049518-21049537 None:intergenic 30.0%
ATCAAACGGTTCATATGTAA+TGG - chr2.2:21049053-21049072 MS.gene068656:intron 30.0%
CCTAAAGAATGTTCGTATAA+AGG - chr2.2:21051173-21051192 MS.gene068656:CDS 30.0%
CCTTTATACGAACATTCTTT+AGG + chr2.2:21051176-21051195 None:intergenic 30.0%
GAGAATGAACTAGGAAAAAT+TGG - chr2.2:21049075-21049094 MS.gene068656:intron 30.0%
GAGGAACTTACTAGAAAAAA+GGG - chr2.2:21049409-21049428 MS.gene068656:intron 30.0%
GATTCATGTGATTATCGTTA+CGG - chr2.2:21049455-21049474 MS.gene068656:intron 30.0%
TACCATTCAATTCAAAATCG+AGG + chr2.2:21050823-21050842 None:intergenic 30.0%
TATTGGAATTTATGCGTAAC+AGG - chr2.2:21049244-21049263 MS.gene068656:intron 30.0%
TGAGGAACTTACTAGAAAAA+AGG - chr2.2:21049408-21049427 MS.gene068656:intron 30.0%
TTACGAAATCACATTTCTCA+AGG + chr2.2:21049705-21049724 None:intergenic 30.0%
TTTCTTATATACTCTATCCC+AGG + chr2.2:21051375-21051394 None:intergenic 30.0%
TTTGAAGATTCATTAGTTGG+GGG + chr2.2:21050886-21050905 None:intergenic 30.0%
! ACTATTCAATATGGTGTTGA+TGG + chr2.2:21050452-21050471 None:intergenic 30.0%
! AGAGTATTCAAAATCTGTTG+GGG + chr2.2:21049604-21049623 None:intergenic 30.0%
! TATGTAATGGAGAATGAACT+AGG - chr2.2:21049066-21049085 MS.gene068656:intron 30.0%
! TTTGGTTTCTGCATTTAACA+TGG - chr2.2:21049795-21049814 MS.gene068656:intron 30.0%
!! GAGAGTATTCAAAATCTGTT+GGG + chr2.2:21049605-21049624 None:intergenic 30.0%
!! TGAGAGTATTCAAAATCTGT+TGG + chr2.2:21049606-21049625 None:intergenic 30.0%
!!! AACCATTGATTTACCTTTTG+AGG - chr2.2:21049513-21049532 MS.gene068656:intron 30.0%
!!! ATTTTTTGTGATGGCTCAAA+AGG + chr2.2:21050282-21050301 None:intergenic 30.0%
!!! CTCTGTGCAATTTGATTTTT+TGG - chr2.2:21050519-21050538 MS.gene068656:intron 30.0%
!!! TTTTTTGTGATGGCTCAAAA+GGG + chr2.2:21050281-21050300 None:intergenic 30.0%
AAACTGGAGAAAACCTCAAA+AGG + chr2.2:21049529-21049548 None:intergenic 35.0%
AAGGGAATCATACTTGCATT+AGG + chr2.2:21050263-21050282 None:intergenic 35.0%
ACGACTTTAGCATTTGTCTT+TGG + chr2.2:21048792-21048811 None:intergenic 35.0%
ACTAGAAAAAAGGGTGATGA+AGG - chr2.2:21049418-21049437 MS.gene068656:intron 35.0%
AGGGAATCATACTTGCATTA+GGG + chr2.2:21050262-21050281 None:intergenic 35.0%
AGGGACACAAATCAAAGTTA+TGG - chr2.2:21048618-21048637 MS.gene068656:CDS 35.0%
AGTAATCCAAATTGACCTAG+AGG + chr2.2:21051068-21051087 None:intergenic 35.0%
ATGTGATTTCGTAACAGATG+AGG - chr2.2:21049711-21049730 MS.gene068656:intron 35.0%
CAAAATCTGTTGGGGATTAA+GGG + chr2.2:21049596-21049615 None:intergenic 35.0%
CAACTCAAGAACCTTAAAAC+TGG + chr2.2:21049545-21049564 None:intergenic 35.0%
CAATATTTGTAGCATGAGGA+AGG + chr2.2:21050482-21050501 None:intergenic 35.0%
CAATTCAAAATCGAGGCAAA+GGG + chr2.2:21050816-21050835 None:intergenic 35.0%
CTAATGCTACAACTCTTCAA+GGG + chr2.2:21049344-21049363 None:intergenic 35.0%
CTTTGGTCAAAATTAAGGCT+AGG + chr2.2:21048775-21048794 None:intergenic 35.0%
GAATACCAGACTTGATTGAA+AGG - chr2.2:21049480-21049499 MS.gene068656:intron 35.0%
GACACAAATCAAAGTTATGG+AGG - chr2.2:21048621-21048640 MS.gene068656:CDS 35.0%
GCATACTATAGGTTACCAAT+TGG + chr2.2:21050430-21050449 None:intergenic 35.0%
GGCTTGTCTAGAGATATAAA+GGG + chr2.2:21048985-21049004 None:intergenic 35.0%
GTAATCCAAATTGACCTAGA+GGG + chr2.2:21051067-21051086 None:intergenic 35.0%
GTTCGTATAAAGGGATACAA+AGG - chr2.2:21051183-21051202 MS.gene068656:CDS 35.0%
TCAAAATCTGTTGGGGATTA+AGG + chr2.2:21049597-21049616 None:intergenic 35.0%
TCAATTCAAAATCGAGGCAA+AGG + chr2.2:21050817-21050836 None:intergenic 35.0%
TCATGAAGATCTGATCTTAG+AGG - chr2.2:21049849-21049868 MS.gene068656:intron 35.0%
TCTAATGCTACAACTCTTCA+AGG + chr2.2:21049345-21049364 None:intergenic 35.0%
TGCTGCATTACAATTCTACT+AGG - chr2.2:21050116-21050135 MS.gene068656:CDS 35.0%
TTCCAAGTGAAGTATAGTAG+AGG + chr2.2:21050007-21050026 None:intergenic 35.0%
TTCCTCTACTATACTTCACT+TGG - chr2.2:21050002-21050021 MS.gene068656:intron 35.0%
! ATATAAGCTAGAAGTGCATC+AGG + chr2.2:21048673-21048692 None:intergenic 35.0%
! ATGCAAGCTTTTGACAACAA+GGG - chr2.2:21049942-21049961 MS.gene068656:intron 35.0%
! TATCCATTGATCTGAAACCT+GGG - chr2.2:21051355-21051374 MS.gene068656:CDS 35.0%
! TGAAATTTGTGGTGGCATTT+TGG - chr2.2:21049972-21049991 MS.gene068656:intron 35.0%
! TGAGGTTTTCTCCAGTTTTA+AGG - chr2.2:21049531-21049550 MS.gene068656:intron 35.0%
! TGCCTCGATTTTGAATTGAA+TGG - chr2.2:21050818-21050837 MS.gene068656:intron 35.0%
! TGGCTTGTCTAGAGATATAA+AGG + chr2.2:21048986-21049005 None:intergenic 35.0%
! TTATCCATTGATCTGAAACC+TGG - chr2.2:21051354-21051373 MS.gene068656:CDS 35.0%
!! AAATAATGGGTGAAGGAGAA+GGG - chr2.2:21048599-21048618 MS.gene068656:CDS 35.0%
!! TACGAACATTCTTTAGGTTG+TGG + chr2.2:21051170-21051189 None:intergenic 35.0%
!! TCTGAAGAAATAATGGGTGA+AGG - chr2.2:21048592-21048611 MS.gene068656:CDS 35.0%
ACAGGTAGATCTTGAAAGTG+AGG - chr2.2:21049262-21049281 MS.gene068656:intron 40.0%
CACACACACACACACATATA+TGG + chr2.2:21050941-21050960 None:intergenic 40.0%
CAGACTTGATTGAAAGGATG+CGG - chr2.2:21049486-21049505 MS.gene068656:intron 40.0%
CATTTCTCCACTTCCTTACA+CGG - chr2.2:21048693-21048712 MS.gene068656:CDS 40.0%
CGTATAAAGGGATACAAAGG+TGG - chr2.2:21051186-21051205 MS.gene068656:CDS 40.0%
GAGTGCAAAAGAAAGAGCTA+TGG + chr2.2:21050855-21050874 None:intergenic 40.0%
GTCTAGAGATATAAAGGGAC+TGG + chr2.2:21048980-21048999 None:intergenic 40.0%
TAAGATTAACAGGCGAGTCA+AGG - chr2.2:21050215-21050234 MS.gene068656:CDS 40.0%
TATCCCAGGTTTCAGATCAA+TGG + chr2.2:21051361-21051380 None:intergenic 40.0%
TCATGTGATTATCGTTACGG+AGG - chr2.2:21049458-21049477 MS.gene068656:intron 40.0%
TGCAAGGTAGAAGTTTAGTC+TGG + chr2.2:21048839-21048858 None:intergenic 40.0%
TGCTTAGGATGAACTCCAAT+TGG - chr2.2:21050412-21050431 MS.gene068656:CDS 40.0%
TTTCAGCCAAAACAAACCTC+CGG + chr2.2:21051124-21051143 None:intergenic 40.0%
! GATGCAAGCTTTTGACAACA+AGG - chr2.2:21049941-21049960 MS.gene068656:intron 40.0%
!! GAAATAATGGGTGAAGGAGA+AGG - chr2.2:21048598-21048617 MS.gene068656:CDS 40.0%
!! GTTTTTGGATGACTGCTCAT+GGG - chr2.2:21051278-21051297 MS.gene068656:CDS 40.0%
!! TGGTGTTGATGGCATACTAT+AGG + chr2.2:21050441-21050460 None:intergenic 40.0%
AACTTCTACCTTGCAGTGAC+TGG - chr2.2:21048844-21048863 MS.gene068656:CDS 45.0%
AAGATTCATTAGTTGGGGGC+AGG + chr2.2:21050882-21050901 None:intergenic 45.0%
AATCTGTTGGGGATTAAGGG+AGG + chr2.2:21049593-21049612 None:intergenic 45.0%
AATGACTGTCTTCCTTACCC+CGG - chr2.2:21051102-21051121 MS.gene068656:intron 45.0%
ACATTTAACCCTCACCCTCT+AGG - chr2.2:21051050-21051069 MS.gene068656:intron 45.0%
ACTTCTACCTTGCAGTGACT+GGG - chr2.2:21048845-21048864 MS.gene068656:CDS 45.0%
ATCTGTTGGGGATTAAGGGA+GGG + chr2.2:21049592-21049611 None:intergenic 45.0%
CAAATTGACCTAGAGGGTGA+GGG + chr2.2:21051061-21051080 None:intergenic 45.0%
CGCATCCTTTCAATCAAGTC+TGG + chr2.2:21049488-21049507 None:intergenic 45.0%
TCTTAGAGGCCCCAAAACTT+CGG - chr2.2:21049863-21049882 MS.gene068656:intron 45.0%
TGATTGTCTTAGGGTCATCG+AGG - chr2.2:21050755-21050774 MS.gene068656:intron 45.0%
TTCAGCCAAAACAAACCTCC+GGG + chr2.2:21051123-21051142 None:intergenic 45.0%
TTTGTGATGTGAACGAGCGT+AGG - chr2.2:21049770-21049789 MS.gene068656:intron 45.0%
! ACGTAGAACCCGAAGTTTTG+GGG + chr2.2:21049875-21049894 None:intergenic 45.0%
! CACGTAGAACCCGAAGTTTT+GGG + chr2.2:21049876-21049895 None:intergenic 45.0%
! TGTGAACGAGCGTAGGATTT+TGG - chr2.2:21049777-21049796 MS.gene068656:intron 45.0%
!! ATGGTGGTAGCTTTGATGAG+TGG - chr2.2:21051397-21051416 MS.gene068656:CDS 45.0%
!! GGTTTTTGGATGACTGCTCA+TGG - chr2.2:21051277-21051296 MS.gene068656:CDS 45.0%
!! TGGTGGTAGCTTTGATGAGT+GGG - chr2.2:21051398-21051417 MS.gene068656:CDS 45.0%
!! AATTCTTTATATAAATTATT+TGG + chr2.2:21050653-21050672 None:intergenic 5.0%
ACGACTTCCGTGTAAGGAAG+TGG + chr2.2:21048703-21048722 None:intergenic 50.0%
CAAAACAAACCTCCGGGGTA+AGG + chr2.2:21051117-21051136 None:intergenic 50.0%
CCAAATTGACCTAGAGGGTG+AGG + chr2.2:21051062-21051081 None:intergenic 50.0%
CCTCACCCTCTAGGTCAATT+TGG - chr2.2:21051059-21051078 MS.gene068656:intron 50.0%
CTTAGAGGCCCCAAAACTTC+GGG - chr2.2:21049864-21049883 MS.gene068656:intron 50.0%
GTTCTCACGACTTCCGTGTA+AGG + chr2.2:21048709-21048728 None:intergenic 50.0%
TCAGCCAAAACAAACCTCCG+GGG + chr2.2:21051122-21051141 None:intergenic 50.0%
TGAGAACAAGTGTGCTCTCC+AGG - chr2.2:21048722-21048741 MS.gene068656:CDS 50.0%
TGCCTGAGAGTTGTGCAAGT+GGG - chr2.2:21049369-21049388 MS.gene068656:intron 50.0%
TGTGCAAGTGGGGATGTTGT+GGG - chr2.2:21049380-21049399 MS.gene068656:intron 50.0%
TTGCCTGAGAGTTGTGCAAG+TGG - chr2.2:21049368-21049387 MS.gene068656:intron 50.0%
TTGTGCAAGTGGGGATGTTG+TGG - chr2.2:21049379-21049398 MS.gene068656:intron 50.0%
TTGTGGCTGACACACCTACA+CGG + chr2.2:21051153-21051172 None:intergenic 50.0%
! GCACGTAGAACCCGAAGTTT+TGG + chr2.2:21049877-21049896 None:intergenic 50.0%
! GGTCATCGAGGTTCTAGCAT+GGG - chr2.2:21050767-21050786 MS.gene068656:intron 50.0%
!! CTTACCCCGGAGGTTTGTTT+TGG - chr2.2:21051115-21051134 MS.gene068656:intron 50.0%
AAGTGTGCTCTCCAGGCGAT+GGG - chr2.2:21048729-21048748 MS.gene068656:CDS 55.0%
CCACAATGATTCCCATCGCC+TGG + chr2.2:21048743-21048762 None:intergenic 55.0%
CCAGGCGATGGGAATCATTG+TGG - chr2.2:21048740-21048759 MS.gene068656:CDS 55.0%
GCCTGAGAGTTGTGCAAGTG+GGG - chr2.2:21049370-21049389 MS.gene068656:intron 55.0%
GGCTGAAAAGAGACCCGTGT+AGG - chr2.2:21051136-21051155 MS.gene068656:intron 55.0%
GTGCAAGTGGGGATGTTGTG+GGG - chr2.2:21049381-21049400 MS.gene068656:intron 55.0%
TACGTTGCCCAGTCACTGCA+AGG + chr2.2:21048855-21048874 None:intergenic 55.0%
TCCCCACTTGCACAACTCTC+AGG + chr2.2:21049374-21049393 None:intergenic 55.0%
TGCAGTGACTGGGCAACGTA+AGG - chr2.2:21048855-21048874 MS.gene068656:CDS 55.0%
TGTGGCTGACACACCTACAC+GGG + chr2.2:21051152-21051171 None:intergenic 55.0%
! GGGTCATCGAGGTTCTAGCA+TGG - chr2.2:21050766-21050785 MS.gene068656:intron 55.0%
CAAGTGGGGATGTTGTGGGG+TGG - chr2.2:21049384-21049403 MS.gene068656:intron 60.0%
CAAGTGTGCTCTCCAGGCGA+TGG - chr2.2:21048728-21048747 MS.gene068656:CDS 60.0%
GACTGTCTTCCTTACCCCGG+AGG - chr2.2:21051105-21051124 MS.gene068656:intron 60.0%
GGGATGTTGTGGGGTGGATG+AGG - chr2.2:21049390-21049409 MS.gene068656:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 21048589 21051437 21048589 ID=MS.gene068656
chr2.2 mRNA 21048589 21051437 21048589 ID=MS.gene068656.t1;Parent=MS.gene068656
chr2.2 exon 21051150 21051437 21051150 ID=MS.gene068656.t1.exon1;Parent=MS.gene068656.t1
chr2.2 CDS 21051150 21051437 21051150 ID=cds.MS.gene068656.t1;Parent=MS.gene068656.t1
chr2.2 exon 21050522 21050760 21050522 ID=MS.gene068656.t1.exon2;Parent=MS.gene068656.t1
chr2.2 CDS 21050522 21050760 21050522 ID=cds.MS.gene068656.t1;Parent=MS.gene068656.t1
chr2.2 exon 21050033 21050480 21050033 ID=MS.gene068656.t1.exon3;Parent=MS.gene068656.t1
chr2.2 CDS 21050033 21050480 21050033 ID=cds.MS.gene068656.t1;Parent=MS.gene068656.t1
chr2.2 exon 21049110 21049259 21049110 ID=MS.gene068656.t1.exon4;Parent=MS.gene068656.t1
chr2.2 CDS 21049110 21049259 21049110 ID=cds.MS.gene068656.t1;Parent=MS.gene068656.t1
chr2.2 exon 21048589 21048954 21048589 ID=MS.gene068656.t1.exon5;Parent=MS.gene068656.t1
chr2.2 CDS 21048589 21048954 21048589 ID=cds.MS.gene068656.t1;Parent=MS.gene068656.t1
Gene Sequence

>MS.gene068656

ATGTCTGAAGAAATAATGGGTGAAGGAGAAGGGACACAAATCAAAGTTATGGAGGAAGAAGAAGATTTGATCAGCAATCTTCCTGATGCACTTCTAGCTTATATCATTTCTCCACTTCCTTACACGGAAGTCGTGAGAACAAGTGTGCTCTCCAGGCGATGGGAATCATTGTGGAAACACACTCCTAGCCTTAATTTTGACCAAAGACAAATGCTAAAGTCGTTGATCAAAGAAAACAATCAAAATTCCAGACTAAACTTCTACCTTGCAGTGACTGGGCAACGTAAGGTACATTGTTATAAAAATTCATGTAAAAGTAGTTATCTGTTTTTTACTATAAACTTTAGGTTTTTAATTTATGATTGTTTTTTTTTCGTGTTGATCGTGTCCAGTCCCTTTATATCTCTAGACAAGCCATTAGATAATCTTCACAAAAAATTATAAATATCTCAATAATTAGATTAATCAAACGGTTCATATGTAATGGAGAATGAACTAGGAAAAATTGGAGAGAATATGATTACATATTTTAGCTTCCATTTTTCTAATAAAATAGATGAACTTTAATCCTAAAGGCTTTTCAAATATGATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATTGGAATTTATGCGTAACAGGTAGATCTTGAAAGTGAGGAACTTTTTGCTGAAGCAGCCATGATGATTACATCAAATATTGACAATCATATTGGTCCCTTGAAGAGTTGTAGCATTAGACACTTGCCTGAGAGTTGTGCAAGTGGGGATGTTGTGGGGTGGATGAGGAACTTACTAGAAAAAAGGGTGATGAAGGTTTCAATAGAACGTGATTCATGTGATTATCGTTACGGAGGAATACCAGACTTGATTGAAAGGATGCGGCAGCAACCATTGATTTACCTTTTGAGGTTTTCTCCAGTTTTAAGGTTCTTGAGTTGAAGAACTATCATTTTTTAACTACACCCTCCCTTAATCCCCAACAGATTTTGAATACTCTCACTCTTAACAAAGTGAATGTTATATCAAACACTTTTCATGATATTCTCTCTCACTGTTCATCTCTTGAAAATCTTATCCTTGAGAAATGTGATTTCGTAACAGATGAGGTTAAGATTGTAAGTCCAAGCCTTAAATATATTAAGATTTGTGATGTGAACGAGCGTAGGATTTTGGTTTCTGCATTTAACATGGAAGTCATAGAGATTGACTCCGTTATTTGTAATCATGAAGATCTGATCTTAGAGGCCCCAAAACTTCGGGTTCTACGTGCTTACAATAATGTTCAAATGCTTGGACAAAATGTTTTTATAGATGGATGCAAGCTTTTGACAACAAGGGATATTATTGAAATTTGTGGTGGCATTTTGGTAAGTGTTTCCTCTACTATACTTCACTTGGAAAGTTAAACAATGTCAATTTTTTGATTGATGGGTATATTACATTTCTTTCTAGTTAATAGAGGTGCATGTAATCTAATGTTTTAAATTTTTGCTGCATTACAATTCTACTAGGATATATATAAAATTTAATATATGGTTCATATTCAATTACAATTGGATGTTACAATCATGTACATAGATGCAAACATTAAGATTAACAGGCGAGTCAAGGATAATGATTAAACTTTAATATCCCTAATGCAAGTATGATTCCCTTTTGAGCCATCACAAAAAATCACACTTATTGGATAATACTCCATATAAAAAGTTCATAGCTTATTATATGTGGACTAATTGAGTAATTGTGATAGGATAATAAAACTTGTTGTTTATGGTATCTATTTAATGCTTAGGATGAACTCCAATTGGTAACCTATAGTATGCCATCAACACCATATTGAATAGTAATAAAACCTTCCTCATGCTACAAATATTGATTAGAGTTAGAAAACTCTCTGTGCAATTTGATTTTTTGGCTTTGAAAAATATCATGTAAAAATTAGACAATTATATTTTAATTTTTGACCTATCATGAGTATCTATTGTTTAGATCATTAAGTAATGCTAATTAAATTTTACAACAACCAAATAATTTATATAAAGAATTGAAATATTTGTTGTATCATCAAAATGTAACATTACTATATTTTATGAACATTTATGTCTATTTGTTTTATAATTATATTTTATGATTGTCTTAGGGTCATCGAGGTTCTAGCATGGGAAGTATCTTTAAAAATTTGGTTACCCTTTGCCTCGATTTTGAATTGAATGGTAATAGAAATGCCATAGCTCTTTCTTTTGCACTCAAGTCCTGCCCCCAACTAATGAATCTTCAAATAAATAACCGGGTATGCTTATAATTAATTTAGCCATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGATTTCTCGTATTTTTTATTTTAGTTTGATCATTATTGCAGTCATTATGCTTAACTATATTTTTTTTGACATTTAACCCTCACCCTCTAGGTCAATTTGGATTACTATGATCAAAATGACAATGACTGTCTTCCTTACCCCGGAGGTTTGTTTTGGCTGAAAAGAGACCCGTGTAGGTGTGTCAGCCACAACCTAAAGAATGTTCGTATAAAGGGATACAAAGGTGGAGAGTTTGAAATTGAATTTGTTAAGTACTTAATATTAAATGGAGGAGTTATGAATAATATTACAATTTGGTTTTTGGATGACTGCTCATGGGATGAAGTCGTAGCAACTGCATGTTTACAGTCATATCTGAAGCTCTCTCCGAAATTATCCATTGATCTGAAACCTGGGATAGAGTATATAAGAAAATATGGTGGTAGCTTTGATGAGTGGGTCCGAACCCTAAAATAA
Protein sequence

>MS.gene068656.t1

MSEEIMGEGEGTQIKVMEEEEDLISNLPDALLAYIISPLPYTEVVRTSVLSRRWESLWKHTPSLNFDQRQMLKSLIKENNQNSRLNFYLAVTGQRKVDLESEELFAEAAMMITSNIDNHIGPLKSCSIRHLPESCASGDVVGWMRNLLEKRVMKVSIERDSCDYRYGGIPDLIERIFKVLELKNYHFLTTPSLNPQQILNTLTLNKVNVISNTFHDILSHCSSLENLILEKCDFVTDEVKIVSPSLKYIKICDVNERRILVSAFNMEVIEIDSVICNHEDLILEAPKLRVLRAYNNVQMLGQNVFIDGCKLLTTRDIIEICGGILGHRGSSMGSIFKNLVTLCLDFELNGNRNAIALSFALKSCPQLMNLQINNRVNLDYYDQNDNDCLPYPGGLFWLKRDPCRCVSHNLKNVRIKGYKGGEFEIEFVKYLILNGGVMNNITIWFLDDCSWDEVVATACLQSYLKLSPKLSIDLKPGIEYIRKYGGSFDEWVRTLK