AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069389

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069389.t1 MTR_2g081980 95.374 562 25 1 1 562 1 561 0.0 1114
MS.gene069389.t1 MTR_2g082060 73.732 552 129 5 11 560 18 555 0.0 838
MS.gene069389.t1 MTR_2g082010 70.650 569 156 4 2 562 7 572 0.0 826
MS.gene069389.t1 MTR_8g007515 67.794 562 176 4 3 562 2 560 0.0 781
MS.gene069389.t1 MTR_4g019550 67.075 571 159 7 3 558 2 558 0.0 746
MS.gene069389.t1 MTR_2g082050 64.535 516 125 4 42 557 1 458 0.0 648
MS.gene069389.t1 MTR_4g081460 46.181 563 288 10 3 558 8 562 5.25e-176 509
MS.gene069389.t1 MTR_6g008560 45.126 554 294 8 8 558 11 557 7.50e-175 506
MS.gene069389.t1 MTR_5g062230 44.107 560 304 7 1 558 1 553 6.64e-168 488
MS.gene069389.t1 MTR_6g039440 43.393 560 308 7 1 558 1 553 3.50e-167 486
MS.gene069389.t1 MTR_4g048460 45.027 553 295 7 8 558 16 561 7.59e-166 483
MS.gene069389.t1 MTR_3g464190 45.636 550 291 7 11 558 14 557 2.91e-164 479
MS.gene069389.t1 MTR_5g073200 44.000 550 300 7 11 558 6 549 1.63e-163 477
MS.gene069389.t1 MTR_5g073260 43.907 558 304 8 4 558 1 552 2.26e-161 471
MS.gene069389.t1 MTR_5g094620 42.553 564 291 11 1 558 1 537 1.04e-153 451
MS.gene069389.t1 MTR_4g048460 45.916 453 238 5 8 459 16 462 3.49e-139 412
MS.gene069389.t1 MTR_6g008560 45.185 405 216 5 156 558 1 401 2.25e-125 374
MS.gene069389.t1 MTR_4g045810 36.232 552 330 14 13 557 44 580 1.41e-99 313
MS.gene069389.t1 MTR_2g064425 34.301 551 345 10 9 556 48 584 1.14e-97 309
MS.gene069389.t1 MTR_8g057020 33.274 559 335 11 12 556 53 587 6.58e-94 299
MS.gene069389.t1 MTR_7g010710 32.668 551 354 10 10 555 14 552 8.10e-90 287
MS.gene069389.t1 MTR_2g064425 33.212 551 336 12 9 556 48 569 3.57e-88 283
MS.gene069389.t1 MTR_2g065450 33.090 547 350 8 12 556 56 588 4.65e-88 283
MS.gene069389.t1 MTR_2g089120 32.576 528 329 12 35 556 57 563 6.60e-87 280
MS.gene069389.t1 MTR_2g089130 32.950 522 326 11 35 552 57 558 1.00e-86 280
MS.gene069389.t1 MTR_2g064425 39.601 351 201 6 9 358 48 388 1.90e-78 254
MS.gene069389.t1 MTR_4g092010 30.709 508 337 10 51 554 60 556 6.36e-77 254
MS.gene069389.t1 MTR_2g064425 37.892 351 192 8 9 358 48 373 6.26e-69 229
MS.gene069389.t1 MTR_4g092020 31.273 534 321 15 41 556 64 569 5.86e-68 230
MS.gene069389.t1 MTR_2g010960 28.797 507 341 8 38 532 1 499 2.98e-66 224
MS.gene069389.t1 MTR_3g465090 28.723 470 320 7 70 531 31 493 9.79e-63 215
MS.gene069389.t1 MTR_3g063170 27.619 525 357 10 38 552 1 512 1.54e-61 211
MS.gene069389.t1 MTR_3g052120 28.846 520 345 11 44 552 7 512 1.76e-61 211
MS.gene069389.t1 MTR_3g063120 28.205 468 326 7 70 531 6 469 1.31e-60 208
MS.gene069389.t1 MTR_6g064980 28.602 472 318 10 70 531 31 493 1.96e-60 209
MS.gene069389.t1 MTR_3g063140 26.953 512 344 9 29 531 3 493 2.54e-59 206
MS.gene069389.t1 MTR_6g065040 26.904 394 273 7 147 531 4 391 3.33e-47 170
MS.gene069389.t1 MTR_5g094530 40.796 201 84 2 167 366 2 168 3.96e-42 150
MS.gene069389.t1 MTR_2g064295 28.651 541 315 21 63 560 252 764 4.80e-42 161
MS.gene069389.t1 MTR_3g058160 28.491 530 322 20 63 556 61 569 5.97e-42 159
MS.gene069389.t1 MTR_3g058160 28.491 530 322 20 63 556 252 760 4.00e-41 159
MS.gene069389.t1 MTR_3g058160 28.491 530 322 20 63 556 272 780 4.55e-41 159
MS.gene069389.t1 MTR_3g058160 28.491 530 322 20 63 556 265 773 5.92e-41 158
MS.gene069389.t1 MTR_3g058160 26.860 551 325 20 63 556 272 801 1.02e-37 149
MS.gene069389.t1 MTR_7g050990 44.595 148 73 1 202 349 29 167 2.55e-37 148
MS.gene069389.t1 MTR_7g057340 39.884 173 101 3 388 558 1 172 3.75e-37 135
MS.gene069389.t1 MTR_3g063160 29.667 300 194 4 31 329 5 288 1.29e-36 138
MS.gene069389.t1 MTR_7g057390 37.572 173 102 4 388 558 1 169 3.99e-33 124
MS.gene069389.t1 MTR_2g012870 23.551 552 394 10 20 553 211 752 1.07e-31 130
MS.gene069389.t1 MTR_2g012900 24.028 566 388 15 20 553 211 766 1.27e-29 124
MS.gene069389.t1 MTR_7g094970 27.798 277 172 8 63 314 204 477 8.95e-23 103
MS.gene069389.t1 MTR_7g011663 29.021 286 171 9 63 323 342 620 3.96e-21 98.2
MS.gene069389.t1 MTR_7g011770 26.099 364 228 12 63 397 238 589 9.11e-20 93.6
MS.gene069389.t1 MTR_5g030050 27.778 270 152 9 71 313 4 257 1.91e-19 89.7
MS.gene069389.t1 MTR_3g015080 29.070 172 83 4 388 558 1 134 6.66e-14 69.3
MS.gene069389.t1 MTR_5g081730 39.175 97 58 1 162 258 16 111 4.83e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069389.t1 AT5G23960 39.162 549 326 7 11 558 4 545 1.75e-135 405
MS.gene069389.t1 AT5G23960 38.949 552 325 9 11 558 4 547 1.06e-133 400
MS.gene069389.t1 AT3G14490 36.727 550 335 6 14 558 60 601 2.43e-117 360
MS.gene069389.t1 AT1G70080 33.982 565 355 12 5 558 54 611 6.32e-103 323
MS.gene069389.t1 AT3G14520 34.483 551 346 10 14 558 63 604 4.22e-102 321
MS.gene069389.t1 AT3G14540 33.874 555 352 9 10 558 56 601 2.26e-101 319
MS.gene069389.t1 AT3G32030 33.880 549 352 7 14 558 63 604 2.80e-100 316
MS.gene069389.t1 AT5G44630 34.559 544 338 11 22 556 21 555 4.07e-100 314
MS.gene069389.t1 AT1G31950 33.818 550 349 10 15 558 66 606 4.95e-100 315
MS.gene069389.t1 AT1G31950 33.757 551 349 11 15 558 66 607 6.70e-100 315
MS.gene069389.t1 AT1G33750 33.333 561 361 9 1 558 53 603 3.54e-98 310
MS.gene069389.t1 AT3G29410 32.299 548 360 7 14 558 64 603 2.10e-92 295
MS.gene069389.t1 AT4G16740 31.972 563 357 15 6 558 18 564 1.40e-91 292
MS.gene069389.t1 AT4G16730 33.013 521 328 9 50 558 28 539 2.08e-89 286
MS.gene069389.t1 AT4G13300 31.502 546 360 9 19 558 17 554 2.06e-88 283
MS.gene069389.t1 AT2G24210 31.625 566 351 14 12 559 44 591 8.06e-88 283
MS.gene069389.t1 AT4G20210 31.835 556 365 9 9 558 53 600 8.70e-87 281
MS.gene069389.t1 AT3G14540 33.696 460 293 6 10 465 56 507 2.88e-86 276
MS.gene069389.t1 AT4G16730 32.600 500 317 8 71 558 2 493 4.54e-86 276
MS.gene069389.t1 AT4G15870 32.784 546 336 14 22 558 75 598 1.99e-85 277
MS.gene069389.t1 AT3G29410 31.818 506 334 7 56 558 1 498 1.06e-84 272
MS.gene069389.t1 AT1G31950 34.071 452 285 8 15 461 66 509 1.14e-84 273
MS.gene069389.t1 AT3G25830 30.634 568 361 12 10 559 43 595 4.25e-83 271
MS.gene069389.t1 AT3G25820 30.634 568 361 12 10 559 43 595 4.25e-83 271
MS.gene069389.t1 AT3G25810 30.851 564 370 11 9 559 42 598 4.60e-83 271
MS.gene069389.t1 AT4G20230 31.767 532 345 11 33 554 78 601 1.91e-81 267
MS.gene069389.t1 AT4G20230 31.767 532 345 11 33 554 90 613 2.47e-81 267
MS.gene069389.t1 AT4G13280 31.238 541 358 9 24 558 22 554 2.96e-79 259
MS.gene069389.t1 AT4G20200 32.245 490 319 9 74 558 123 604 9.63e-79 259
MS.gene069389.t1 AT1G61680 31.631 509 329 8 47 548 73 569 8.87e-78 256
MS.gene069389.t1 AT2G23230 30.090 555 368 12 10 554 52 596 7.00e-77 255
MS.gene069389.t1 AT1G61680 31.434 509 323 8 47 548 73 562 1.34e-76 253
MS.gene069389.t1 AT2G23230 29.910 555 364 12 10 554 52 591 4.03e-75 250
MS.gene069389.t1 AT1G48800 30.055 549 367 12 14 554 63 602 5.41e-74 247
MS.gene069389.t1 AT3G29110 30.182 550 368 11 14 556 55 595 2.86e-72 242
MS.gene069389.t1 AT1G66020 30.435 552 368 11 14 558 56 598 3.12e-72 242
MS.gene069389.t1 AT3G29190 30.518 521 347 10 45 558 89 601 6.64e-70 236
MS.gene069389.t1 AT4G16740 35.278 360 217 9 6 358 18 368 1.71e-69 229
MS.gene069389.t1 AT4G13280 29.630 540 338 10 24 558 22 524 5.47e-66 224
MS.gene069389.t1 AT5G48110 34.012 344 219 7 217 558 2 339 5.70e-63 210
MS.gene069389.t1 AT1G48820 29.167 552 326 16 14 554 63 560 6.79e-63 216
MS.gene069389.t1 AT3G25820 30.657 411 256 9 2 397 31 427 3.82e-62 211
MS.gene069389.t1 AT4G16740 35.135 333 200 9 6 331 18 341 2.78e-61 207
MS.gene069389.t1 AT4G16740 35.135 333 200 9 6 331 18 341 2.78e-61 207
MS.gene069389.t1 AT1G66020 33.144 353 225 7 24 371 66 412 1.20e-51 183
MS.gene069389.t1 AT3G25820 31.014 345 211 8 2 331 31 363 1.41e-51 182
MS.gene069389.t1 AT3G25820 31.014 345 211 8 2 331 31 363 1.41e-51 182
MS.gene069389.t1 AT1G79460 25.142 529 340 20 67 560 274 781 7.02e-27 116
MS.gene069389.t1 AT1G61120 24.568 521 343 18 67 558 285 784 1.57e-25 112
MS.gene069389.t1 AT4G02780 29.167 312 180 10 19 301 288 587 5.06e-22 100

Find 87 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTTTGCTCGATGCTTAT+AGG 0.184362 2.3:+15490039 None:intergenic
CCTACTTTGTTGAATCAAAA+TGG 0.254857 2.3:-15490639 MS.gene069389:CDS
CCACATTCCTCGTACATCTT+TGG 0.258760 2.3:+15494256 None:intergenic
CATTGGTGATAAGGGAATTA+AGG 0.268338 2.3:+15494156 None:intergenic
TGTACGAAGCTGCACAATTA+AGG 0.268522 2.3:-15494229 MS.gene069389:CDS
CATTGACTCATTGCAACGTT+TGG 0.277670 2.3:-15495364 MS.gene069389:CDS
ATGTAATCTTTCAGCAGTTC+TGG 0.298577 2.3:+15490081 None:intergenic
TTTCGTTTGCTTAGGCAAAA+AGG 0.303378 2.3:-15495219 MS.gene069389:CDS
GCAAGGAAATCGAGAATTAT+TGG 0.311853 2.3:-15490227 MS.gene069389:CDS
TAGAAAGATGGTGGGAAAAT+TGG 0.325709 2.3:-15491575 MS.gene069389:CDS
TTTCTAGCTGTGCTATATTT+AGG 0.334197 2.3:+15491592 None:intergenic
TATGATGAATACAAGGCTAA+TGG 0.381392 2.3:-15490592 MS.gene069389:CDS
ATAAATTCAAGCACTGGTCT+TGG 0.384389 2.3:+15490165 None:intergenic
GACTCAATTCATTGGTGATA+AGG 0.387271 2.3:+15494147 None:intergenic
ATTGACTCATTGCAACGTTT+GGG 0.407695 2.3:-15495363 MS.gene069389:CDS
CATAGTTGTTTAGCAAGAAA+AGG 0.421143 2.3:+15494125 None:intergenic
ACTCAATTCATTGGTGATAA+GGG 0.421569 2.3:+15494148 None:intergenic
AACAATCATCGATGCTATAT+CGG 0.425469 2.3:-15490473 MS.gene069389:CDS
AGGTGTCATATTTCTTCCTA+TGG 0.429937 2.3:-15494053 MS.gene069389:CDS
GCCACTCTATCCCTTGCGTA+TGG 0.435962 2.3:+15491652 None:intergenic
GAGTGGCTGAGCCTTACTTT+TGG 0.440451 2.3:-15491636 MS.gene069389:CDS
AACACCACTTTCATACTCTC+TGG 0.451212 2.3:+15490873 None:intergenic
CCTTAGCATTTCGTTTGCTT+AGG 0.453704 2.3:-15495227 MS.gene069389:CDS
TAAATTCAAGCACTGGTCTT+GGG 0.457483 2.3:+15490166 None:intergenic
AAGAAAAGGACTCAATTCAT+TGG 0.464501 2.3:+15494139 None:intergenic
GTTTGCATACGAAAACTTTG+AGG 0.467064 2.3:-15490118 MS.gene069389:CDS
GATATAGCATCGATGATTGT+TGG 0.468446 2.3:+15490475 None:intergenic
CTAGGACTAAAATCTACAAT+TGG 0.470398 2.3:+15500409 None:intergenic
GATGATACTTATGATGCCTA+TGG 0.473959 2.3:-15491532 MS.gene069389:CDS
ATTGGTCGCTTGATATCAAC+TGG 0.476391 2.3:+15500427 None:intergenic
GCCTTGTATTCATCATATGT+TGG 0.481369 2.3:+15490598 None:intergenic
GCTGCACAATTAAGGATTCA+TGG 0.483650 2.3:-15494221 MS.gene069389:CDS
ATAAAAGATAATATTTGAAG+TGG 0.485664 2.3:+15490550 None:intergenic
ATAGCCAATGGCCAAAAGTA+AGG 0.500107 2.3:+15491625 None:intergenic
GGAGAATGGAACATCAAGTT+TGG 0.506101 2.3:-15490805 MS.gene069389:CDS
GAAGAGGTGTCTTTAAGCAT+TGG 0.512416 2.3:+15494102 None:intergenic
GAAATAATAACCACAAAAGA+AGG 0.516368 2.3:-15495267 MS.gene069389:CDS
GGCTTATAACTTCATTCGCA+AGG 0.517699 2.3:-15490244 MS.gene069389:CDS
TGATGACATATCCTCACATA+AGG 0.519182 2.3:-15490434 MS.gene069389:intron
TTCATAATAAATTCAAGCAC+TGG 0.524438 2.3:+15490159 None:intergenic
GAGACAACAATTGTGGAGAA+TGG 0.524699 2.3:-15490819 MS.gene069389:CDS
CGCTTGATATCAACTGGTGC+GGG 0.526655 2.3:+15500433 None:intergenic
AAGCTCTTCAACCGTGCCAT+AGG 0.531005 2.3:+15491516 None:intergenic
CTCTTCACAAGGGAGTTCCT+AGG 0.531180 2.3:-15494085 MS.gene069389:CDS
CCACTCTATCCCTTGCGTAT+GGG 0.532210 2.3:+15491653 None:intergenic
CAAACGATGAGATTCTCGAT+TGG 0.539109 2.3:-15490510 MS.gene069389:CDS
ATGAAGCAATATGACATGTC+AGG 0.541915 2.3:-15490273 MS.gene069389:CDS
GGATATCACATTTCATCAGA+TGG 0.551267 2.3:-15495198 MS.gene069389:intron
TTGGGATGTTGTCTAACTTG+AGG 0.553837 2.3:+15490184 None:intergenic
ATGCTAAGGCAAGGAAGTGA+AGG 0.555119 2.3:+15495241 None:intergenic
ATATGACATGTCAGGAGAAG+AGG 0.555557 2.3:-15490265 MS.gene069389:CDS
TGACACCTTGTCTCTAACCT+AGG 0.565384 2.3:+15494068 None:intergenic
AGAAGAACTTCACTATGTGA+AGG 0.567042 2.3:+15494023 None:intergenic
GGAAATCGAGAATTATTGGA+TGG 0.568775 2.3:-15490223 MS.gene069389:CDS
CTTAAAGACACCTCTTCACA+AGG 0.569618 2.3:-15494096 MS.gene069389:CDS
CCACTTTCATACTCTCTGGA+AGG 0.571471 2.3:+15490877 None:intergenic
CCTTCCAGAGAGTATGAAAG+TGG 0.572615 2.3:-15490877 MS.gene069389:CDS
CCTAAGCAAACGAAATGCTA+AGG 0.578368 2.3:+15495227 None:intergenic
ATATAGCACAGCTAGAAAGA+TGG 0.581470 2.3:-15491587 MS.gene069389:CDS
TTGGTTGAGAGACATATTGA+AGG 0.581659 2.3:+15500368 None:intergenic
ATCGATGCTATATCGGCTCA+TGG 0.582437 2.3:-15490466 MS.gene069389:CDS
TCGCTTGATATCAACTGGTG+CGG 0.584343 2.3:+15500432 None:intergenic
CCAAAGATGTACGAGGAATG+TGG 0.585780 2.3:-15494256 MS.gene069389:CDS
TCAATATGTCTCTCAACCAA+TGG 0.586194 2.3:-15500365 MS.gene069389:intron
TAGCACAGCTAGAAAGATGG+TGG 0.589965 2.3:-15491584 MS.gene069389:CDS
CACTTTCATACTCTCTGGAA+GGG 0.591620 2.3:+15490878 None:intergenic
CAACAAACGTCCCATACGCA+AGG 0.594870 2.3:-15491663 MS.gene069389:CDS
CTAGGAACTCCCTTGTGAAG+AGG 0.595991 2.3:+15494086 None:intergenic
TCCAACATATGATGAATACA+AGG 0.599952 2.3:-15490599 MS.gene069389:CDS
GCTTGAATTTATTATGAATG+TGG 0.603828 2.3:-15490154 MS.gene069389:CDS
GAATCAAAATGGTGCAGTGA+AGG 0.606039 2.3:-15490628 MS.gene069389:CDS
CCCATACGCAAGGGATAGAG+TGG 0.607579 2.3:-15491653 MS.gene069389:CDS
ATGTGAAGGTTCTTCACCAT+AGG 0.611070 2.3:+15494037 None:intergenic
GCAAACGAAATGCTAAGGCA+AGG 0.623931 2.3:+15495232 None:intergenic
GGCTCATAAGACATAGCCAA+TGG 0.624278 2.3:+15491613 None:intergenic
TAAAAGATAATATTTGAAGT+GGG 0.624513 2.3:+15490551 None:intergenic
AGCACAGCTAGAAAGATGGT+GGG 0.631900 2.3:-15491583 MS.gene069389:CDS
TGAAATTGAGACAACAATTG+TGG 0.651963 2.3:-15490826 MS.gene069389:CDS
TGCTAAGGCAAGGAAGTGAA+GGG 0.653445 2.3:+15495242 None:intergenic
AAGAGTGCATGTTGCTTCTG+CGG 0.654921 2.3:-15490307 MS.gene069389:CDS
AACAAACGTCCCATACGCAA+GGG 0.657873 2.3:-15491662 MS.gene069389:CDS
AGTGCATGTTGCTTCTGCGG+TGG 0.663575 2.3:-15490304 MS.gene069389:CDS
AATATGCAGTACCTTATGTG+AGG 0.683021 2.3:+15490423 None:intergenic
GAGTTCCTAGGTTAGAGACA+AGG 0.684119 2.3:-15494073 MS.gene069389:CDS
TACTTATGATGCCTATGGCA+CGG 0.715446 2.3:-15491527 MS.gene069389:CDS
AATCTTTCCAAAGATGTACG+AGG 0.725278 2.3:-15494263 MS.gene069389:CDS
TTAAAGACACCTCTTCACAA+GGG 0.753099 2.3:-15494095 MS.gene069389:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.3 gene 15490035 15500473 15490035 ID=MS.gene069389
chr2.3 mRNA 15490035 15500473 15490035 ID=MS.gene069389.t1;Parent=MS.gene069389
chr2.3 exon 15500366 15500473 15500366 ID=MS.gene069389.t1.exon1;Parent=MS.gene069389.t1
chr2.3 CDS 15500366 15500473 15500366 ID=cds.MS.gene069389.t1;Parent=MS.gene069389.t1
chr2.3 exon 15495199 15495487 15495199 ID=MS.gene069389.t1.exon2;Parent=MS.gene069389.t1
chr2.3 CDS 15495199 15495487 15495199 ID=cds.MS.gene069389.t1;Parent=MS.gene069389.t1
chr2.3 exon 15493954 15494329 15493954 ID=MS.gene069389.t1.exon3;Parent=MS.gene069389.t1
chr2.3 CDS 15493954 15494329 15493954 ID=cds.MS.gene069389.t1;Parent=MS.gene069389.t1
chr2.3 exon 15491490 15491708 15491490 ID=MS.gene069389.t1.exon4;Parent=MS.gene069389.t1
chr2.3 CDS 15491490 15491708 15491490 ID=cds.MS.gene069389.t1;Parent=MS.gene069389.t1
chr2.3 exon 15490779 15490923 15490779 ID=MS.gene069389.t1.exon5;Parent=MS.gene069389.t1
chr2.3 CDS 15490779 15490923 15490779 ID=cds.MS.gene069389.t1;Parent=MS.gene069389.t1
chr2.3 exon 15490435 15490680 15490435 ID=MS.gene069389.t1.exon6;Parent=MS.gene069389.t1
chr2.3 CDS 15490435 15490680 15490435 ID=cds.MS.gene069389.t1;Parent=MS.gene069389.t1
chr2.3 exon 15490035 15490340 15490035 ID=MS.gene069389.t1.exon7;Parent=MS.gene069389.t1
chr2.3 CDS 15490035 15490340 15490035 ID=cds.MS.gene069389.t1;Parent=MS.gene069389.t1
Gene Sequence

>MS.gene069389

ATGGAATCTCTTGCAGCTCCCGCACCAGTTGATATCAAGCGACCAATTGTAGATTTTAGTCCTAGCATTTGGGGGGATGTATTCCTTCAATATGTCTCTCAACCAATGGTACTGTTACTTTCACATGCAATGTTTTACGTACATATATATGCGTTAGTTTAAATATTGTTACTAATTATACTATCTTATAGTAGCATTTTAACGAGACATATCTTATACTGTATTGGCCTTTGATGTTATAATCTAGTTCGATTCCTATCCAATTATGGGATCACAAACTACAATCTTTAAACTACAAAAAAAAAAAACTAGAATCTTTCTTGGAGATCTTATTCAAGATGATACTTCATATGCTTTTTGTGGTGCTCTCAGTTGAGTCGACATCTGTTTGTTACTTGTCAGTCTATTCTATCCATGGTGGAGTTTTTAGCATAGTAACGGGGCTCGGGGTGGGAGGAGGGTCAGGTTCAGGGGCATGATTGTTTTTCTACGACTTTTCTGTTTCCGTATTGGTGTGGGGCTTAGATGATTGTTAAAACATGTGTGTCAAAATCTTGAATGACATTTATTAAGTGAGAGAGCAGCTTTCTTTTGTTTTATACACCCTCCGGTCACAAATATAAGCAAAAATTCACTTTTAAGGTTCATTCAATAAATGATGTATGTGGACTATTAATAGTCCACATACATCATTTATTGAATGAACTTTAAAAATGAATTTTTGCTTATATTTATGACCGGAGGGTGTACTTTATTTTCATAGTGTAGTTAATGTGTTTAATTTGTGTGCGAATTTTACTAAATTGTTGTTATTTGTATGTGTATTTTATTTTATTTTTTATATTTTAATGCAAGTTAATAGTATTAATTAGGATATCTTTAAAAAAAATGCACATAATAATTTGTATAGAGATTTAGATTTAGGGACTAGATTTTTTTTTTAAAAAAAAAAACTTATAATTAGGGACGGATGGGGAAGTAATTGTTTTATTAATTGTAGTTGACTAGTTGTATCCTTCTATGACCTTTACTTGGCTAGAGGATTGCTTTTATTTTTGCGTATCTCTCCTCCTAGTGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTCACATAGCTCAGTTGATAGGGACATGCATTATTATATGCAGGGGTCGGGGTTCGAACCCCAGACACCCCACTTATTCACTTTGAAAAAGGTGAATTCTAGCCACTAGACTACTTGAAAGAAAAAAAAAAAAAAGAATTATAGATACATACACATAGATCTTAGCCATTCAAAAATAAAAGGCCAGCCATTCAATATGTAAGTCACAAAAACTAGGAAGGATTTTTTATACGCAAAACCATTAATGCGAGTATAGTTTCGTTCACCATTAATGTTGAAAATAAATTGAGTTGAGTCGAATTTATTTCAGTTTTATTTGGCTCAAAACTCGACTCGAGTTTGTCACCAATATTTTGTTCAGCTCATACTCAACTCGATTCTAGTTCTTGAGTAATTTGATTTAACTCGTTGGTTCGAATCAAATGAACCAATAGACAAAAAGAAAACTATTTTTTTAAGAAAAACACTATAAATCGACAAATGAGAGTATGTGGAGTGTTAACGTTTGCTATATAATTTAAATTAAAAATCCATTTTAAATGTGGGAATTAGTGTAACCTTCGGAGAAAAAAAAATACGACCAATATTTTCTTTTAAATTAGTGTAATTGATATAGCAATGAAAAACGATATTTATATATATCAAGTTGTCTCATGTATCAAACGAGTCGAAATACACATAACTCAAGTCCAACTCGTTTATTGAACGAGCTTATGTTTCAACTCAAGTTCGACTCATTTGATCTACAAACCAAGCTCAACGAATTATTTACCGAATCAAATCTCGAATTATTTTCGAATTGGTTCGGTTCATTGTCAACCGTATTCACCACTGCAACTCCATCGCATACCACCATCGATAGCTACCGTGGACGGTCATCATACTTTTATAAAAAAAAAATAATAAAATTACTGATTTAAAATTATTTTAAAAAATTATAATATTTCAAAGACAGATCTTTTTTGGGATGAATTAACCAAAAGAAAAAAAAAAGCAAAAACATGTTTTCAAAATATTTTAAAAATTAGTTAAAAATATCCTTGAATTTTTTTTATAAACTTTAATAGATGGATAGAATGAATATCCATATATTGAAAATATAAAATGGATGGATTGGATGAGATTTTTTAAAAGAATTTTCAATGAATTGTTAATGAATTAAGAAATTGTTTTAATTCGTTCATTACCATCCAAATTCTTCCAATCCATTAATTTTCCCACCATTTCACAATTCCACTTTACCGGATACCATTTCAATAGGAGTGATCAAAATCGAAACAACCCAATAGAAAACCTGTAAACGAAACCAACCCAAATCGAAAATTGCAAAAACCGCATTTGGTTCAGACATATATATTTGGACCATGTTTTAACTGAACCATATGGTTCGGTTCACTCTGCAGTTTTTATTTTACCAATCGAGGCAAAACGAACCAAGTCACAACATAACATTAATTAAAATTTTGTCACTCAACCTAGCACTCATAGAAATTCGATGTAAATTTTATAGAATGACTTTTTTTTTTTATTAAGACATTTTTTTTTCTTGTTAAGTTTCTTCTATGTTTTTTATATCAAAGATGTATTTATGTGCTTTAAGTTAATTCTGCTTGATTATTTGCATGTGTGTGTTAGATATATCCTGAATCTTAGAACTCAATGAAAACTTTTAGAGAATGGTAAAATCTTAATGTCTTTTGTATTTCTAATCGAAGAGAGTTGCTTTTTGGTCCATGTTGACAGAAAATGAAATTGTATAGTTACACCAAGCAACCCAAATTGTATTGGTTTGGTTTGGTTTGATTTTGTTTTTTATTTTACTAAAAGTTAATTGGACCAAACCAAACCGTATGGTTTTTTATCTTACGATTCAGATGACTTTTAGCCTATAGAACTCACGTGTATATCACCTAATAAAAGAGTAAATGTTGAAAAGAGAGTTTGCTAGCAATCCTAAAAATGAAAGAGAATATTGACTAGTTCAGGATAGTGCATTTTTAAAATAATGAAACTTAACTTTTGACATTTTTATTGTTTTCAGAACATGTTTACAATTCACTTCAAGTGTCTCTTATCTATTTGTTTATAAAATAAAAGGTTAAAATATTTTTGGTCCCTGCAAAATGGAGACCTTCTTAAATTTAATCCTTACTTAACTTTAGTAGTTTTTATAGTCCTTAAAAAAAATTTATGCACTCAATTTCAATCCTTGCTAAAACAAATTTATTTAATTATCATTAAACTCTTTGGTTTTTGAATGATATTTTTACACACAACTTTAGAACATAATAAATAAAAAGTTATTCCGCAAAAAAAAGCAATTGCAATATTTATTTTTTAGGTCTAGATTTTGGTTACATTTATTTTGATCGGTTGACATTTAACAAATCATATTTAATTATCTTACGAACATGTGTGTATAAGGGACAGAAGTTTGTATAAGGGATAATTCAAGCACTTTTATTTTAACAAAAATTACATTTTTTTAATATATAAAAATGTTAAGACGACTTATAATAAAGAGTAATGATATTTGAACAACCATTTCATACAACTTTTGGTGACAACTTTTTTTTCTCTTTTTTCATTGGTTAAAAACAATGGAGAGAGAAAAAGGAAGAGAGAGAATAAGAGGATAATGTGAGTATGAGAGAGAAAATTATCAAAAAGTTGTCACAAAGTGGTTGTACAAATATCATTTCTCTATAATAAAATACATAGACCAATTAATAAGTATTTTCAATAAATGAACTTTTATAATGTTACGAACATGTGTGAATAAGGGACAGAAGTCTGTATAAGGGACAATTCAAGCATTTTGTTTTAACAAAAACTATATTTTTTAAATATATATAGGGTTTAGGACATAGACCAATTATTAAAAAGTATGATACATGAGTATAAAATACGTGGAGCAAATTAATGAGTACGATTTACGTGTTCTATTTTAGATTTCGTATTTTACTTTGTTTTTGTGAAAGGAAAAATATTGTAAGTTGTGGTCGCTCGTAGGATGCCCTTAAAAATGAATTAAATCATTTTAGACTTCGTTTTTTATTTTTATGAAAGGAAAAATATTGTAAGTTGTGGTCGCTCGTAGGATGTCCTTAAAAATGAATTAAATCATCTTTTTTTTTTGTGTTCGGGGTTCGAACTCCGAAGCTTACATTAATTATATATTAATGCATTGTCTCTAGTAACTGAGTTAAGCTTATGAGGACGAATAATGACCCGTTTGATTTATATTTATAGATTGGGTCTGTTTGATGGTCAGGATAGAGAGACATAATATGATACATTAATCATATCCCGCTAAGATCTCATGTTTGATGCACCAAAGGGATATGATAAAATAAACCATTTTTCTATCATATCCTGACTTTCCTTTGTAATTTCTATCCTGACTATAGGCTGGGTTAGAAATTATCCTGATGGGCTTAGAAGATCATTCATTATATATTATCTTGATATTCTTCCATTTTTTTTAAAATATTGTCATTTTTAAAATAAATGATAGCATGTATTTTCTATTATAATTAATAACATGTCATTATTACAATATTTAATTAATTATGAGGCAATTTTTTTCATTTAATTATAATGATATCCTGTTTGTTATCATGTACGCATCAAACAGATGATAGGATTAATAGTTATCATGTCATTATCATATCCTATCTCTATCCAACTTTATATCATATTCTGTCACTATCTTATCCTGGTCATCAAACGGAATGCTATATAAATATTCCATTCCTTGTGACCAATATTACTTGCAGGAAATCGACGAAAATATGAAGAAACAAGTGCAAATGCAAAAAGAGGAAGTGAGGAAGATTTTTCAATCTTCAAGCAATGATATTTCACAAAAGTTAAACTTCATTGACTCATTGCAACGTTTGGGAATATCTTATCATTTTGAACGTGAAATTGATGAAGCATTAGAACAAATCTACAAGAATTTAACTAAAAATAAGGAAATAATAACCACAAAAGAAGGTTCCCTTCACTTCCTTGCCTTAGCATTTCGTTTGCTTAGGCAAAAAGGATATCACATTTCATCAGATGGTAAGTAGAATTTTTTACGCATATTTATAGGGGTGTAAGTGGTTCGGATAAAACCAACAAAATCGAAAGTCCAAACCGAACCAAACCGAAAAAATATCCAACCTTGTTTGGTTCGGTTTGAAACCGATCCGAACCAATCAAAACCGATCTAGTTTGGTTTGGTTCATGGTTTTTCATTTTGAGATCCGTGAAACCGATGAACCAATCCGATGATAACCGCACTCCTTATTTTACTAATATTATTCTACCTCACACAAATTTATGGTTAGGCCCAAAATTAAATTTCATGATGTTTTAATTTCAGTTTGCCTGTATTTGTTATTTTGACTATGTTTTAATTTTAGGATGTTCTTTTTGGAATGTTTGTGTTTGTTGATGAATGATGCATGTATGGATCATGAATGATTGAAATATTTGAGTTTGATGAAGTTGTTTTTAAAGAATGATGGATGAATGAATCTTGGATCATATGTATGAAATATTGAGAAAGGTTAGTTATAAAAAATTGTTTGAAAAATAGAAAACATGAAAAATCAAAATATGAAATACAATTATACAAAGATCTATGGAATATTTTTGTGAGAATATGAATTTTTTTTAATTGTATTTTTAAATAAACCGATCAACCAAACCAATCCAAACCGAACCAAACCGATTAGTTTGGTTTGGTTTCTTTTTTGAAAAACAGTAAAAACTGAACCAATCCAAACCGATGAAGGTTTCATCGGTTCGGACATTTTTTTGACTGAAAACCAATACAAACCAAACCGTTACACCCCTGCATATTTAACTTCATAGCCATTAATAATAATAATACAATAAAATCTCACAAGGTTAATGTTAATAATGTGGGTGAGTTTTTTTGACAGAGATATTTGAAAAATTCAAAAACAATAAAGGAAGCTTGAATGAAAATCTTTCCAAAGATGTACGAGGAATGTGGAGTTTGTACGAAGCTGCACAATTAAGGATTCATGGTGAAGATATATTAGATGAAGCACTTGATTTCACATACTCTCACCTTAATTCCCTTATCACCAATGAATTGAGTCCTTTTCTTGCTAAACAACTATGCCAATGCTTAAAGACACCTCTTCACAAGGGAGTTCCTAGGTTAGAGACAAGGTGTCATATTTCTTCCTATGGTGAAGAACCTTCACATAGTGAAGTTCTTCTAAAGTTTGCAAAACTAGATTTCAACACGTTGCAGAAAATGCATCAAAAAGAAATCGGCAGCATCACCAAGTAAGTATAAAAAGGATTTGAGAATAGTCTATATATACATTTGAATTTTGATAACATTTGAATTTTGATACAAAGTGTCATTATGAACATTTGACATTGTCAACATAACCATTAATATATACGACTTTAGAAATAGTTATAATTACTCAAATAGCTTCAATGATTTGATCGGTCATATGAACCAATTTGTGAAATTTGAAAAAAATTGTATATGAAAAAATTTGGGGACCAATTTGTGAAATTTGAAAAAATATATATGAACCAATTTGTCAAACAGTTTAACCACTTAAATTTTAAATAATTGTATACTGACCCCCTAAATCTTCAAAGACTATTACTTTTCTCACTCTACTGTCTTCTCACGCGCCCTGCAAAAATTCCAAAAATACCACTGTTAGTGCTTTCTAGATTTTAAAATCCGGACAAAATCGATATAAAAAGGGTCTGCTCTTTTCTTCAATATGACGCCTTTTCTCTTCCAATTTCTTCCCCATCTTCTTCCTCAAATCAAATACCCCTGTTTGCAATTCTCTAACAAAGGCTTTGTAAGATCTTGCAAACTGTTGCTATTCTTTAGTGTTGCTGTACGAGAGGAACTTACTGGTGGTACTAAAGAATCTGATGGTTTACTAGTTGGTGCTTAAGTAGATTCAACACTCTTAGGTTTTTGTTGTTTGTTAGCTGGACCATAACTTGAAGTGCTTGCACTAGCTTCCGACCTAGCTTTTGCTCCACTAGAAGATTGATTATTGGTATTAATCGAAGTGGAAGTCTCATTCCTATTTTCTTGGTTAATCCATATGAAGGAATTTGTTGATTTCAATTTTCAATGGATGGATTCGGATTACATAATCCGGATGTTTTTACACATTCCGGATTATATAATCTGAAATATGTAAATCACCCAGTTGAAAGTCAATAAATAGGGTGAAAAATGATGTTTCCGGATTACATAATCCGGAATGCGTTAAAACAATTCGGATTTTATAATCCGGAATGTGATTATATGGAGGATTTCTCTAGTATGACCAATATTTTTTTTTTCAGAAACAAGCTTTGTATTGCATAAAAAATGGTTGGGATGAATAAATCCTTGGAACCTGGTGTTGGAGTACTTATTTCTCTACATAACAATGCTCAAAGTATGAAGCTGACATATCCAGAACTCTTGTGATGAATGAGAAAAGTAAAGTTTCAATACTCACTTCAAAGCATTAGAATCATGAGTCATGAATTTTAAAGATTTCAAATTCCAAATTAACTTGCCTCGTCAAGAGCTTAAAAAATATCAATGCTTGGTTGGTGAATTGTGCACACAACAGTTCTCCCTTGTCTACTGTGTTTCTCACAGTTCGCATCACAATAGCATCAAGGCCTGATGTTGGTTCATCATGAAGATGATTGAAGGGTTAGCAACCAATTCCACAACAATAGAAAGCTTAAAATAAAAAAATTCTGGAAAAACACCATTCCGGATTATAAAATCCGGAAAGCTCCGAGGGTAATTTTGGAAAAAAAATAGGGCGCGCGAGGAAAGACATAGGGTGGGAAAAGTAATAGTCTTCTTCAAAATGTGCAAATTAGTCTGTAAATTCAAATTTGACCCAAAACTTGTAAAATTTATAAAAGCAGACCCATAATGTTAGCACTGAAAGTTTATACTACCCTATCCAAACTAAGATACTTAGATATGAAGAAAATTCAATTGAACCACTTGAATTGTCTAAAATTCTAAATTCTAAAAAAAAATTCATTTCATCATCCAAACACATTCATAAGAAATTTTACTTTATAAATTAAACATTTCTCTGCTTTATTTCTTTACTCTAAACAAGCTAAATCCATCTAATATATTAAATTACTATTGAATTAATGGAAGATCAACTTATAATATATAGGCAAAATAATACTTATGGTCTCTTAAATAGTTTTAGATAACAATTTGGTCTTTTATCTTTTTTCAATTTTATTTTTGCCCTTTTAGTTTATTTGTAATGAATTTTTAAGTTTCTATAATCTATATGGGTTTCTTTTCTTATGATCTTTCAATCTTCAAATCTATAGTCTTTTATCTATGTTTGCATACAAATATTTGATTTGAAGTTTGAAAATTCATATACAAATGACACAGCTAGAAAGATGGTGGTGGAAAAACTCAGAATTATTATATGAAATTAAGTTAATAGACTATAAACGTAATATTTCTCAATATATATGATATTAATTAGTTGTATTTATACTGTAGGTGGTGGAAAAACTCAGAATTTGCAACAAACGTCCCATACGCAAGGGATAGAGTGGCTGAGCCTTACTTTTGGCCATTGGCTATGTCTTATGAGCCTAAATATAGCACAGCTAGAAAGATGGTGGGAAAATTGGTAGCATGTGTTTCTCTTTTAGATGATACTTATGATGCCTATGGCACGGTTGAAGAGCTTGAACTTTTCACAGAGGCAATGAAAAGGTTGATTCGAGTCCAAAAAGATATTGACTAATTTTATTCTATTTTTTTACAATATGACAATTAATGACCAGATGACGACGTTCAATCATTAACATGAGCATGAGTTAACTTTGTAAATGTTTTTTTTTTTTTTTTAAGAAGGTTACTTTGAAATGGTTAAACCTGTATTTTCTTTAGAAAAAGATAAACCACACAAGTGTTGGTGACTTAGATTTTTCTACCTAAACTTCATATTTTTCTGTGTGGTGTCAGGGGTTCGAACACCGGACCTTACATATATTATGTATTGTCCATCAACTGAGTTAAGGTCACCAAAACATCTTAATATAAACAAATAGGTCATGAGATGGGGTTTTATTCAAATTCTATTATGTAAAACTAACACTTTTAAAAAGTATGGTCATAATTTGGGACTAACATATTGCCCGTCTCAAATTATAAATCTCTTTAAAGAGTTCTAATCAAAACGACTTATAATTTAAGATGCGTTTGAACTTAATTAGAATTTTGTTTTTATTTTCTATGTATTTGGCTCATTTATAATTCTCTTGTGTTCTGAATTTCAGGTGGGATATTAATGTCATTCAATCCCTTCCAGAGAGTATGAAAGTGGTGTTTAATGCAATTGTTGAACTGTGTGATGAAATTGAGACAACAATTGTGGAGAATGGAACATCAAGTTTGGTGTTTATACAATATGTCAAACAAAATGTAGGCTTACAAATTCATATATTATTTCAAAATTACCAATTGTGCTTAATTTTGCGCTTTCAGTAAATTAGTTGATCAATATTTTTATTAATTTACAGTTTTACAAATTGGCACGATCCTACTTTGTTGAATCAAAATGGTGCAGTGAAGGATATATTCCAACATATGATGAATACAAGGCTAATGGATCTATATCTTCTTCATACCCACTTCAAATATTATCTTTTATTGGGCTTGGAGAATTTTCAAACGATGAGATTCTCGATTGGATTTTCAACTATCCAACAATCATCGATGCTATATCGGCTCATGGCAGACTCGCTGATGACATATCCTCACATAAGGTACTGCATATTTTGTATCGTCTGCATATGAAAGTACATCGAAAGTATCTAACAAAACTGAAGTATACATATTTTGTATCTGCTTTATTTATAGTTTGAGCAAGAAAGAGTGCATGTTGCTTCTGCGGTGGAATGTTGCATGAAGCAATATGACATGTCAGGAGAAGAGGCTTATAACTTCATTCGCAAGGAAATCGAGAATTATTGGATGGTTATGAATGAAGAGTGCCTCAAGTTAGACAACATCCCAAGACCAGTGCTTGAATTTATTATGAATGTGGCGCGAGTGACCGAGTTTGCATACGAAAACTTTGAGGATAAATACACAAAACCAGAACTGCTGAAAGATTACATTGCTGCACTACTTATAGATCCTATAAGCATCGAGCAAAGTGAGTAG
Protein sequence

>MS.gene069389.t1

MESLAAPAPVDIKRPIVDFSPSIWGDVFLQYVSQPMEIDENMKKQVQMQKEEVRKIFQSSSNDISQKLNFIDSLQRLGISYHFEREIDEALEQIYKNLTKNKEIITTKEGSLHFLALAFRLLRQKGYHISSDEIFEKFKNNKGSLNENLSKDVRGMWSLYEAAQLRIHGEDILDEALDFTYSHLNSLITNELSPFLAKQLCQCLKTPLHKGVPRLETRCHISSYGEEPSHSEVLLKFAKLDFNTLQKMHQKEIGSITKWWKNSEFATNVPYARDRVAEPYFWPLAMSYEPKYSTARKMVGKLVACVSLLDDTYDAYGTVEELELFTEAMKRWDINVIQSLPESMKVVFNAIVELCDEIETTIVENGTSSLVFIQYVKQNFYKLARSYFVESKWCSEGYIPTYDEYKANGSISSSYPLQILSFIGLGEFSNDEILDWIFNYPTIIDAISAHGRLADDISSHKFEQERVHVASAVECCMKQYDMSGEEAYNFIRKEIENYWMVMNEECLKLDNIPRPVLEFIMNVARVTEFAYENFEDKYTKPELLKDYIAALLIDPISIEQSE