AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071185

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071185.t1 MTR_2g096610 88.667 150 15 1 1 150 1 148 6.48e-84 243
MS.gene071185.t1 MTR_4g064967 94.667 150 6 1 1 150 1 148 1.17e-81 237
MS.gene071185.t1 MTR_4g064005 94.000 150 7 1 1 150 1 148 1.72e-81 237
MS.gene071185.t1 MTR_4g071150 92.763 152 9 1 1 150 1 152 2.42e-81 236
MS.gene071185.t1 MTR_4g063280 92.424 132 9 1 20 150 14 145 3.46e-80 233
MS.gene071185.t1 MTR_4g063410 92.000 150 10 1 1 150 1 148 1.49e-79 232
MS.gene071185.t1 MTR_2g096570 88.312 154 13 2 1 150 1 153 7.40e-78 228
MS.gene071185.t1 MTR_8g086640 96.212 132 5 0 1 132 1 132 3.21e-74 218
MS.gene071185.t1 MTR_7g113160 78.947 133 27 1 10 142 13 144 1.05e-69 207
MS.gene071185.t1 MTR_7g108320 73.016 126 34 0 4 129 2 127 5.09e-59 180
MS.gene071185.t1 MTR_7g114060 74.016 127 29 1 3 129 2 124 8.26e-59 179
MS.gene071185.t1 MTR_7g114040 74.016 127 29 1 3 129 2 124 8.26e-59 179
MS.gene071185.t1 MTR_2g082510 74.790 119 30 0 11 129 3 121 2.62e-58 177
MS.gene071185.t1 MTR_7g093910 72.656 128 32 2 3 129 2 127 3.07e-58 177
MS.gene071185.t1 MTR_2g082220 73.950 119 31 0 11 129 3 121 2.51e-57 175
MS.gene071185.t1 MTR_6g005150 63.780 127 32 3 24 149 26 139 3.30e-47 150
MS.gene071185.t1 MTR_7g114050 68.333 120 23 3 17 132 10 118 3.38e-45 144
MS.gene071185.t1 MTR_4g063300 74.286 105 22 1 45 149 10 109 1.27e-44 142
MS.gene071185.t1 MTR_1g011750 59.292 113 44 2 21 132 23 134 1.45e-35 120
MS.gene071185.t1 MTR_1g011850 59.292 113 44 2 21 132 23 134 1.45e-35 120
MS.gene071185.t1 MTR_1g114050 59.633 109 42 2 21 128 20 127 8.83e-35 118
MS.gene071185.t1 MTR_1g071040 60.952 105 39 2 25 128 27 130 4.42e-34 116
MS.gene071185.t1 MTR_1g069770 48.958 96 48 1 45 139 9 104 6.78e-25 92.4
MS.gene071185.t1 MTR_7g010440 70.588 51 15 0 78 128 122 172 1.30e-16 74.7
MS.gene071185.t1 MTR_4g005910 74.419 43 11 0 87 129 4 46 2.27e-16 68.9
MS.gene071185.t1 MTR_4g048490 43.810 105 30 2 24 128 26 101 3.81e-16 69.7
MS.gene071185.t1 MTR_7g027610 41.818 110 38 3 20 128 10 94 3.09e-15 67.8
MS.gene071185.t1 MTR_2g096590 72.500 40 11 0 110 149 12 51 4.60e-12 57.8
MS.gene071185.t1 MTR_0653s0030 36.364 99 53 2 56 146 44 140 9.45e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071185.t1 AT5G02560 82.353 153 24 1 1 150 1 153 6.51e-79 230
MS.gene071185.t1 AT5G02560 71.186 177 24 2 1 150 1 177 4.95e-74 219
MS.gene071185.t1 AT5G27670 75.000 152 34 3 1 150 1 150 2.18e-72 214
MS.gene071185.t1 AT5G59870 73.649 148 38 1 4 150 3 150 2.57e-70 209
MS.gene071185.t1 AT1G51060 71.318 129 37 0 11 139 3 131 1.63e-60 183
MS.gene071185.t1 AT4G27230 70.543 129 38 0 11 139 3 131 7.58e-60 181
MS.gene071185.t1 AT4G27230 70.543 129 38 0 11 139 3 131 7.58e-60 181
MS.gene071185.t1 AT3G20670 71.318 129 37 0 11 139 3 131 1.03e-59 181
MS.gene071185.t1 AT5G54640 72.000 125 35 0 11 135 3 127 4.61e-58 177
MS.gene071185.t1 AT1G08880 73.770 122 29 1 11 129 6 127 9.81e-58 176
MS.gene071185.t1 AT1G54690 73.770 122 29 1 11 129 6 127 1.79e-57 176
MS.gene071185.t1 AT2G38810 60.550 109 41 2 21 128 25 132 1.02e-35 120
MS.gene071185.t1 AT2G38810 60.550 109 41 2 21 128 25 132 1.02e-35 120
MS.gene071185.t1 AT2G38810 60.550 109 41 2 21 128 25 132 1.02e-35 120
MS.gene071185.t1 AT2G38810 60.550 109 41 2 21 128 25 132 1.02e-35 120
MS.gene071185.t1 AT1G52740 61.905 105 38 2 25 128 27 130 2.36e-34 117
MS.gene071185.t1 AT3G54560 57.798 109 44 2 25 132 29 136 1.31e-33 115
MS.gene071185.t1 AT3G54560 57.798 109 44 2 25 132 29 136 1.31e-33 115
MS.gene071185.t1 AT4G13570 47.826 92 46 2 32 122 28 118 7.29e-20 79.7
MS.gene071185.t1 AT4G13570 47.253 91 46 2 33 122 35 124 1.95e-19 79.0

Find 44 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.239817 4.2:+40932482 None:intergenic
GATCAGATCTAAGCCTTCTT+TGG 0.251178 4.2:+40932461 None:intergenic
GGCTCCTTTCTTCGCGGTCT+TGG 0.319054 4.2:+40932886 None:intergenic
CTTGTCACCGACTTCTTCCT+TGG 0.333455 4.2:+40932842 None:intergenic
TGCTGCTGAAGTTCTTGAAT+TGG 0.339223 4.2:-40932696 MS.gene071185:CDS
TTCTTTGGCTCTTTGTTAGC+AGG 0.375405 4.2:+40932497 None:intergenic
AGAACTGCTGCTAGGTATAC+TGG 0.389575 4.2:+40932728 None:intergenic
CAATTTCCAGTTGGAAGAAT+CGG 0.397925 4.2:-40932800 MS.gene071185:CDS
TCTTCTTAGGCAAGAGGATT+GGG 0.402193 4.2:+40932534 None:intergenic
TACGCTAAGCGTGTTGGAAC+AGG 0.407073 4.2:-40932755 MS.gene071185:CDS
AAGAGGATTGGGTTAATGTT+TGG 0.407916 4.2:+40932545 None:intergenic
TTCTTCTTAGGCAAGAGGAT+TGG 0.412247 4.2:+40932533 None:intergenic
GGCATGTGTTGTTGGCTGTT+AGG 0.442957 4.2:-40932628 MS.gene071185:CDS
TCCTCCTCCTTTCCTTCCAC+CGG 0.452471 4.2:+40932865 None:intergenic
AGCACCAAGACCGCGAAGAA+AGG 0.452810 4.2:-40932890 MS.gene071185:CDS
GCTGAAGTTCTTGAATTGGC+TGG 0.461152 4.2:-40932692 MS.gene071185:CDS
AACAAAGAGCCAAAGAAGGC+TGG 0.466354 4.2:-40932491 MS.gene071185:CDS
TATTCCAAGGCATGTGTTGT+TGG 0.470522 4.2:-40932636 MS.gene071185:CDS
AAAGGAGCCGGTGGAAGGAA+AGG 0.476592 4.2:-40932872 MS.gene071185:CDS
TATCGACCGATTCTTCCAAC+TGG 0.484363 4.2:+40932794 None:intergenic
GCCGGTGGAAGGAAAGGAGG+AGG 0.487329 4.2:-40932866 MS.gene071185:CDS
GAGCTTGGTAAATTACTTGC+TGG 0.499780 4.2:-40932596 MS.gene071185:CDS
ACAGCCAACAACACATGCCT+TGG 0.515073 4.2:+40932632 None:intergenic
ATCGGTCGATACTTGAAGAA+AGG 0.519021 4.2:-40932782 MS.gene071185:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 4.2:-40932474 MS.gene071185:CDS
TCCACCGGCTCCTTTCTTCG+CGG 0.538173 4.2:+40932880 None:intergenic
GGAAGATACGCTAAGCGTGT+TGG 0.539997 4.2:-40932761 MS.gene071185:CDS
TCCAACTGGAAATTGAAGAC+CGG 0.540370 4.2:+40932808 None:intergenic
TGCTAACAAAGAGCCAAAGA+AGG 0.544648 4.2:-40932495 MS.gene071185:CDS
TTTCATTCTTCTTAGGCAAG+AGG 0.549215 4.2:+40932528 None:intergenic
CGAAGAAAGGAGCCGGTGGA+AGG 0.552463 4.2:-40932877 MS.gene071185:CDS
GTTAGGAACGATGAAGAGCT+TGG 0.552797 4.2:-40932611 MS.gene071185:CDS
TGGCTCTTTGTTAGCAGGAG+CGG 0.554707 4.2:+40932502 None:intergenic
GAAGGAAAGGAGGAGGACCA+AGG 0.555100 4.2:-40932859 MS.gene071185:CDS
GGAGCCGGTGGAAGGAAAGG+AGG 0.561766 4.2:-40932869 MS.gene071185:CDS
GCCGGTCTTCAATTTCCAGT+TGG 0.566234 4.2:-40932809 MS.gene071185:CDS
AAGACCGCGAAGAAAGGAGC+CGG 0.590011 4.2:-40932884 MS.gene071185:CDS
GATATTCAAGAACTGCTGCT+AGG 0.592671 4.2:+40932720 None:intergenic
GTGACAAGATCAATCAGAGC+CGG 0.601599 4.2:-40932827 MS.gene071185:CDS
ACCGCGAAGAAAGGAGCCGG+TGG 0.603380 4.2:-40932881 MS.gene071185:CDS
AGGAGGACCAAGGAAGAAGT+CGG 0.610829 4.2:-40932849 MS.gene071185:CDS
AGAAGAATAGAATTATTCCA+AGG 0.617592 4.2:-40932649 MS.gene071185:CDS
GCTGGTGTTACAATTGCTCA+CGG 0.638494 4.2:-40932578 MS.gene071185:CDS
GGTGTTACAATTGCTCACGG+TGG 0.811864 4.2:-40932575 MS.gene071185:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGAATAGAATTATTCCA+AGG - chr4.2:40932719-40932738 MS.gene071185:CDS 25.0%
AAGAGGATTGGGTTAATGTT+TGG + chr4.2:40932826-40932845 None:intergenic 35.0%
CAATTTCCAGTTGGAAGAAT+CGG - chr4.2:40932568-40932587 MS.gene071185:CDS 35.0%
TTTCATTCTTCTTAGGCAAG+AGG + chr4.2:40932843-40932862 None:intergenic 35.0%
ATCGGTCGATACTTGAAGAA+AGG - chr4.2:40932586-40932605 MS.gene071185:CDS 40.0%
GAGCTTGGTAAATTACTTGC+TGG - chr4.2:40932772-40932791 MS.gene071185:CDS 40.0%
GATATTCAAGAACTGCTGCT+AGG + chr4.2:40932651-40932670 None:intergenic 40.0%
TATTCCAAGGCATGTGTTGT+TGG - chr4.2:40932732-40932751 MS.gene071185:CDS 40.0%
TCCAACTGGAAATTGAAGAC+CGG + chr4.2:40932563-40932582 None:intergenic 40.0%
TCTTCTTAGGCAAGAGGATT+GGG + chr4.2:40932837-40932856 None:intergenic 40.0%
TGCTAACAAAGAGCCAAAGA+AGG - chr4.2:40932873-40932892 MS.gene071185:CDS 40.0%
TGCTGCTGAAGTTCTTGAAT+TGG - chr4.2:40932672-40932691 MS.gene071185:CDS 40.0%
TTCTTCTTAGGCAAGAGGAT+TGG + chr4.2:40932838-40932857 None:intergenic 40.0%
!! TTCTTTGGCTCTTTGTTAGC+AGG + chr4.2:40932874-40932893 None:intergenic 40.0%
!!! GCGGCTTTTTCATTCTTCTT+AGG + chr4.2:40932850-40932869 None:intergenic 40.0%
AACAAAGAGCCAAAGAAGGC+TGG - chr4.2:40932877-40932896 MS.gene071185:CDS 45.0%
AGAACTGCTGCTAGGTATAC+TGG + chr4.2:40932643-40932662 None:intergenic 45.0%
GCTGAAGTTCTTGAATTGGC+TGG - chr4.2:40932676-40932695 MS.gene071185:CDS 45.0%
GTGACAAGATCAATCAGAGC+CGG - chr4.2:40932541-40932560 MS.gene071185:CDS 45.0%
GTTAGGAACGATGAAGAGCT+TGG - chr4.2:40932757-40932776 MS.gene071185:CDS 45.0%
! TATCGACCGATTCTTCCAAC+TGG + chr4.2:40932577-40932596 None:intergenic 45.0%
!! GCTGGTGTTACAATTGCTCA+CGG - chr4.2:40932790-40932809 MS.gene071185:CDS 45.0%
ACAGCCAACAACACATGCCT+TGG + chr4.2:40932739-40932758 None:intergenic 50.0%
AGGAGGACCAAGGAAGAAGT+CGG - chr4.2:40932519-40932538 MS.gene071185:CDS 50.0%
GCCGGTCTTCAATTTCCAGT+TGG - chr4.2:40932559-40932578 MS.gene071185:CDS 50.0%
GGAAGATACGCTAAGCGTGT+TGG - chr4.2:40932607-40932626 MS.gene071185:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG + chr4.2:40932889-40932908 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG - chr4.2:40932894-40932913 MS.gene071185:CDS 50.0%
TACGCTAAGCGTGTTGGAAC+AGG - chr4.2:40932613-40932632 MS.gene071185:CDS 50.0%
TGGCTCTTTGTTAGCAGGAG+CGG + chr4.2:40932869-40932888 None:intergenic 50.0%
! GGCATGTGTTGTTGGCTGTT+AGG - chr4.2:40932740-40932759 MS.gene071185:CDS 50.0%
! GGTGTTACAATTGCTCACGG+TGG - chr4.2:40932793-40932812 MS.gene071185:CDS 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG + chr4.2:40932529-40932548 None:intergenic 50.0%
AAAGGAGCCGGTGGAAGGAA+AGG - chr4.2:40932496-40932515 MS.gene071185:CDS 55.0%
AAGACCGCGAAGAAAGGAGC+CGG - chr4.2:40932484-40932503 MS.gene071185:CDS 55.0%
AGCACCAAGACCGCGAAGAA+AGG - chr4.2:40932478-40932497 MS.gene071185:CDS 55.0%
GAAGGAAAGGAGGAGGACCA+AGG - chr4.2:40932509-40932528 MS.gene071185:CDS 55.0%
TCCTCCTCCTTTCCTTCCAC+CGG + chr4.2:40932506-40932525 None:intergenic 55.0%
CGAAGAAAGGAGCCGGTGGA+AGG - chr4.2:40932491-40932510 MS.gene071185:CDS 60.0%
GGCTCCTTTCTTCGCGGTCT+TGG + chr4.2:40932485-40932504 None:intergenic 60.0%
!! TCCACCGGCTCCTTTCTTCG+CGG + chr4.2:40932491-40932510 None:intergenic 60.0%
ACCGCGAAGAAAGGAGCCGG+TGG - chr4.2:40932487-40932506 MS.gene071185:CDS 65.0%
GCCGGTGGAAGGAAAGGAGG+AGG - chr4.2:40932502-40932521 MS.gene071185:CDS 65.0%
GGAGCCGGTGGAAGGAAAGG+AGG - chr4.2:40932499-40932518 MS.gene071185:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 40932469 40932921 40932469 ID=MS.gene071185
chr4.2 mRNA 40932469 40932921 40932469 ID=MS.gene071185.t1;Parent=MS.gene071185
chr4.2 exon 40932469 40932921 40932469 ID=MS.gene071185.t1.exon1;Parent=MS.gene071185.t1
chr4.2 CDS 40932469 40932921 40932469 ID=cds.MS.gene071185.t1;Parent=MS.gene071185.t1
Gene Sequence

>MS.gene071185

ATGGACGCAAGCACCAAGACCGCGAAGAAAGGAGCCGGTGGAAGGAAAGGAGGAGGACCAAGGAAGAAGTCGGTGACAAGATCAATCAGAGCCGGTCTTCAATTTCCAGTTGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATACGCTAAGCGTGTTGGAACAGGTGCACCAGTATACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATTATTCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGTAAATTACTTGCTGGTGTTACAATTGCTCACGGTGGTGTTCTTCCAAACATTAACCCAATCCTCTTGCCTAAGAAGAATGAAAAAGCCGCTCCTGCTAACAAAGAGCCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG
Protein sequence

>MS.gene071185.t1

MDASTKTAKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAKRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKNEKAAPANKEPKKAGKSPKKA