Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072327.t1 | XP_003625149.1 | 90.3 | 72 | 7 | 0 | 4 | 75 | 120 | 191 | 8.10E-27 | 130.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072327.t1 | Q7XII4 | 57.9 | 76 | 30 | 2 | 1 | 76 | 156 | 229 | 7.5e-13 | 75.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072327.t1 | A2Q3K8 | 90.3 | 72 | 7 | 0 | 4 | 75 | 120 | 191 | 5.9e-27 | 130.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049452 | MS.gene072327 | 0.802501 | 5.45E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene072327 | 0.809254 | 2.07E-50 | -1.69E-46 |
| MS.gene050680 | MS.gene072327 | 0.829786 | 4.24E-55 | -1.69E-46 |
| MS.gene051368 | MS.gene072327 | 0.819886 | 9.18E-53 | -1.69E-46 |
| MS.gene052190 | MS.gene072327 | 0.812331 | 4.47E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene072327 | 0.855295 | 6.94E-62 | -1.69E-46 |
| MS.gene052586 | MS.gene072327 | 0.809643 | 1.71E-50 | -1.69E-46 |
| MS.gene052867 | MS.gene072327 | 0.800251 | 1.57E-48 | -1.69E-46 |
| MS.gene053166 | MS.gene072327 | 0.823944 | 1.05E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene072327 | -0.827178 | 1.81E-54 | -1.69E-46 |
| MS.gene054559 | MS.gene072327 | 0.807156 | 5.80E-50 | -1.69E-46 |
| MS.gene055228 | MS.gene072327 | 0.806463 | 8.13E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene072327 | 0.83778 | 4.25E-57 | -1.69E-46 |
| MS.gene056506 | MS.gene072327 | 0.800975 | 1.12E-48 | -1.69E-46 |
| MS.gene057012 | MS.gene072327 | 0.805488 | 1.30E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene072327 | 0.828884 | 7.01E-55 | -1.69E-46 |
| MS.gene060310 | MS.gene072327 | 0.803617 | 3.20E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072327.t1 | MTR_7g091990 | 93.000 | 100 | 7 | 0 | 1 | 100 | 117 | 216 | 1.22e-64 | 199 |
| MS.gene072327.t1 | MTR_1g052560 | 67.000 | 100 | 26 | 1 | 1 | 100 | 118 | 210 | 5.56e-36 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072327.t1 | AT2G41870 | 58.824 | 102 | 25 | 3 | 2 | 100 | 121 | 208 | 1.36e-28 | 106 |
| MS.gene072327.t1 | AT3G57540 | 56.522 | 92 | 37 | 1 | 12 | 100 | 139 | 230 | 5.75e-25 | 97.4 |
Find 32 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCCTTTGTCAAGTATTGAT+TGG | 0.397247 | 7.2:-17074050 | None:intergenic |
| ATGCTGTGGTAATAATTAGT+AGG | 0.401149 | 7.2:+17073988 | MS.gene072327:CDS |
| TCACGGTGTTATTTGCATGT+AGG | 0.449599 | 7.2:+17073936 | MS.gene072327:CDS |
| CAATGGATGGGAGAGTGAAC+AGG | 0.460888 | 7.2:+17073854 | MS.gene072327:CDS |
| GGAGACGGAACAGGATCAAA+AGG | 0.469993 | 7.2:-17073654 | None:intergenic |
| AGGTTCAGAAAGCTACTTCA+TGG | 0.476454 | 7.2:+17073874 | MS.gene072327:CDS |
| AACAAACCCTTTGGCGATTG+TGG | 0.479682 | 7.2:+17073620 | MS.gene072327:CDS |
| GTCGGTGGATAGAGTGAAGA+AGG | 0.481158 | 7.2:+17073746 | MS.gene072327:CDS |
| GATGGAAGAAACAAACCCTT+TGG | 0.499116 | 7.2:+17073611 | None:intergenic |
| CTGCTAGTAGGAGACGGAAC+AGG | 0.505373 | 7.2:-17073663 | None:intergenic |
| AAGTTGCTAAAATTAACAAC+AGG | 0.513840 | 7.2:+17073808 | MS.gene072327:CDS |
| AGTATTGATTGGTTCGAAGT+TGG | 0.529886 | 7.2:-17074039 | None:intergenic |
| AGAGATGATGCTGTCATCAA+TGG | 0.530421 | 7.2:+17073837 | MS.gene072327:CDS |
| TCCTACTAGCAGAAGAACTA+CGG | 0.555436 | 7.2:+17073674 | MS.gene072327:CDS |
| TTGTTATGTGTTTCATGCTG+TGG | 0.556156 | 7.2:+17073974 | MS.gene072327:CDS |
| TTATCTACCACAATCGCCAA+AGG | 0.563144 | 7.2:-17073627 | None:intergenic |
| GCCGTAGTTCTTCTGCTAGT+AGG | 0.572201 | 7.2:-17073675 | None:intergenic |
| TATCTACCACAATCGCCAAA+GGG | 0.572915 | 7.2:-17073626 | None:intergenic |
| AGCAGAAGAACTACGGCTGG+TGG | 0.576648 | 7.2:+17073681 | MS.gene072327:CDS |
| ACTAGCAGAAGAACTACGGC+TGG | 0.586567 | 7.2:+17073678 | MS.gene072327:CDS |
| ATGATGCTGTCATCAATGGA+TGG | 0.593521 | 7.2:+17073841 | MS.gene072327:CDS |
| AGTACTCGTTCAAGTGGTCA+AGG | 0.616828 | 7.2:+17073705 | MS.gene072327:CDS |
| GAGACGGAACAGGATCAAAA+GGG | 0.628833 | 7.2:-17073653 | None:intergenic |
| GGTGGAAGTACTCGTTCAAG+TGG | 0.633101 | 7.2:+17073699 | MS.gene072327:CDS |
| GTTCTTCTGCTAGTAGGAGA+CGG | 0.637966 | 7.2:-17073669 | None:intergenic |
| TGATGCTGTCATCAATGGAT+GGG | 0.638726 | 7.2:+17073842 | MS.gene072327:CDS |
| TTGATGCAAAGATATCAGCT+TGG | 0.653643 | 7.2:+17073775 | MS.gene072327:CDS |
| TGGTAGTGAAGAACATGTGT+CGG | 0.675585 | 7.2:+17073728 | MS.gene072327:CDS |
| TAGTGAAGAACATGTGTCGG+TGG | 0.696232 | 7.2:+17073731 | MS.gene072327:CDS |
| TTCATGGATGAAGAAAGTTG+AGG | 0.696748 | 7.2:+17073890 | MS.gene072327:CDS |
| ACTCGTTCAAGTGGTCAAGG+TGG | 0.708848 | 7.2:+17073708 | MS.gene072327:CDS |
| CAGCATGAAACACATAACAA+CGG | 0.730460 | 7.2:-17073971 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGTTGCTAAAATTAACAAC+AGG | + | chr7.2:17073808-17073827 | MS.gene072327:CDS | 25.0% |
| !!! | TTTTTTCTTCTTCTTTCTCA+CGG | + | chr7.2:17073919-17073938 | MS.gene072327:CDS | 25.0% |
| ATGCTGTGGTAATAATTAGT+AGG | + | chr7.2:17073988-17074007 | MS.gene072327:CDS | 30.0% | |
| CAGCATGAAACACATAACAA+CGG | - | chr7.2:17073974-17073993 | None:intergenic | 35.0% | |
| TTCATGGATGAAGAAAGTTG+AGG | + | chr7.2:17073890-17073909 | MS.gene072327:CDS | 35.0% | |
| TTGATGCAAAGATATCAGCT+TGG | + | chr7.2:17073775-17073794 | MS.gene072327:CDS | 35.0% | |
| TTGTTATGTGTTTCATGCTG+TGG | + | chr7.2:17073974-17073993 | MS.gene072327:CDS | 35.0% | |
| !! | AGTATTGATTGGTTCGAAGT+TGG | - | chr7.2:17074042-17074061 | None:intergenic | 35.0% |
| AGAGATGATGCTGTCATCAA+TGG | + | chr7.2:17073837-17073856 | MS.gene072327:CDS | 40.0% | |
| AGGTTCAGAAAGCTACTTCA+TGG | + | chr7.2:17073874-17073893 | MS.gene072327:CDS | 40.0% | |
| ATGATGCTGTCATCAATGGA+TGG | + | chr7.2:17073841-17073860 | MS.gene072327:CDS | 40.0% | |
| TATCTACCACAATCGCCAAA+GGG | - | chr7.2:17073629-17073648 | None:intergenic | 40.0% | |
| TCCTACTAGCAGAAGAACTA+CGG | + | chr7.2:17073674-17073693 | MS.gene072327:CDS | 40.0% | |
| TGATGCTGTCATCAATGGAT+GGG | + | chr7.2:17073842-17073861 | MS.gene072327:CDS | 40.0% | |
| TTATCTACCACAATCGCCAA+AGG | - | chr7.2:17073630-17073649 | None:intergenic | 40.0% | |
| ! | TGGTAGTGAAGAACATGTGT+CGG | + | chr7.2:17073728-17073747 | MS.gene072327:CDS | 40.0% |
| !! | TCACGGTGTTATTTGCATGT+AGG | + | chr7.2:17073936-17073955 | MS.gene072327:CDS | 40.0% |
| AACAAACCCTTTGGCGATTG+TGG | + | chr7.2:17073620-17073639 | MS.gene072327:CDS | 45.0% | |
| AGTACTCGTTCAAGTGGTCA+AGG | + | chr7.2:17073705-17073724 | MS.gene072327:CDS | 45.0% | |
| GAGACGGAACAGGATCAAAA+GGG | - | chr7.2:17073656-17073675 | None:intergenic | 45.0% | |
| GTTCTTCTGCTAGTAGGAGA+CGG | - | chr7.2:17073672-17073691 | None:intergenic | 45.0% | |
| TAGTGAAGAACATGTGTCGG+TGG | + | chr7.2:17073731-17073750 | MS.gene072327:CDS | 45.0% | |
| ACTAGCAGAAGAACTACGGC+TGG | + | chr7.2:17073678-17073697 | MS.gene072327:CDS | 50.0% | |
| ACTCGTTCAAGTGGTCAAGG+TGG | + | chr7.2:17073708-17073727 | MS.gene072327:CDS | 50.0% | |
| CAATGGATGGGAGAGTGAAC+AGG | + | chr7.2:17073854-17073873 | MS.gene072327:CDS | 50.0% | |
| GCCGTAGTTCTTCTGCTAGT+AGG | - | chr7.2:17073678-17073697 | None:intergenic | 50.0% | |
| GGAGACGGAACAGGATCAAA+AGG | - | chr7.2:17073657-17073676 | None:intergenic | 50.0% | |
| GTCGGTGGATAGAGTGAAGA+AGG | + | chr7.2:17073746-17073765 | MS.gene072327:CDS | 50.0% | |
| !! | GGTGGAAGTACTCGTTCAAG+TGG | + | chr7.2:17073699-17073718 | MS.gene072327:CDS | 50.0% |
| AGCAGAAGAACTACGGCTGG+TGG | + | chr7.2:17073681-17073700 | MS.gene072327:CDS | 55.0% | |
| CTGCTAGTAGGAGACGGAAC+AGG | - | chr7.2:17073666-17073685 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 17073612 | 17074064 | 17073612 | ID=MS.gene072327 |
| chr7.2 | mRNA | 17073612 | 17074064 | 17073612 | ID=MS.gene072327.t1;Parent=MS.gene072327 |
| chr7.2 | exon | 17073612 | 17074064 | 17073612 | ID=MS.gene072327.t1.exon1;Parent=MS.gene072327.t1 |
| chr7.2 | CDS | 17073612 | 17074064 | 17073612 | ID=cds.MS.gene072327.t1;Parent=MS.gene072327.t1 |
| Gene Sequence |
| Protein sequence |
