AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072756

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072756.t1 MTR_5g009780 95.019 803 40 0 1 803 1 803 0.0 1528
MS.gene072756.t1 MTR_5g009800 91.436 794 47 2 1 794 1 773 0.0 1452
MS.gene072756.t1 MTR_5g009770 88.778 802 86 2 1 800 1 800 0.0 1447
MS.gene072756.t1 MTR_5g009770 87.630 671 79 2 132 800 1 669 0.0 1198
MS.gene072756.t1 MTR_4g078120 60.468 812 299 6 6 800 3 809 0.0 956
MS.gene072756.t1 MTR_4g123583 59.194 794 308 5 6 786 3 793 0.0 925
MS.gene072756.t1 MTR_8g093780 47.222 792 397 12 4 779 5 791 0.0 716
MS.gene072756.t1 MTR_3g096380 46.230 809 417 10 10 803 13 818 0.0 703
MS.gene072756.t1 MTR_2g011520 44.720 767 414 8 18 779 3 764 0.0 671
MS.gene072756.t1 MTR_7g099800 49.567 809 363 16 15 779 8 815 0.0 666
MS.gene072756.t1 MTR_7g099820 47.595 790 380 14 16 780 5 785 0.0 653
MS.gene072756.t1 MTR_7g099750 48.481 823 344 16 15 779 8 808 0.0 647
MS.gene072756.t1 MTR_7g099820 48.924 697 331 9 16 694 5 694 0.0 624
MS.gene072756.t1 MTR_3g031170 34.319 778 475 11 10 778 28 778 5.18e-138 428
MS.gene072756.t1 MTR_2g040100 33.902 820 483 24 22 802 35 834 4.89e-131 411
MS.gene072756.t1 MTR_3g071990 32.567 783 494 14 6 778 7 765 2.96e-129 405
MS.gene072756.t1 MTR_1g043400 32.290 799 486 20 18 782 11 788 1.00e-122 388
MS.gene072756.t1 MTR_1g045870 32.075 795 478 19 18 782 11 773 2.84e-120 381
MS.gene072756.t1 MTR_0002s1180 31.318 827 510 19 8 798 2 806 2.68e-117 374
MS.gene072756.t1 MTR_5g067690 31.652 793 493 16 19 782 23 795 9.47e-117 373
MS.gene072756.t1 MTR_5g074340 30.818 782 492 17 18 778 30 783 6.21e-116 371
MS.gene072756.t1 MTR_5g074380 31.748 778 486 15 19 778 76 826 8.50e-116 371
MS.gene072756.t1 MTR_5g074360 31.443 776 491 15 19 778 31 781 8.53e-116 370
MS.gene072756.t1 MTR_3g071990 32.764 702 438 14 87 778 6 683 1.02e-114 365
MS.gene072756.t1 MTR_3g071880 29.695 788 510 19 18 777 19 790 2.78e-109 353
MS.gene072756.t1 MTR_3g037270 30.866 797 507 19 9 782 17 792 1.33e-108 351
MS.gene072756.t1 MTR_3g071870 29.639 776 501 15 18 767 32 788 1.38e-108 351
MS.gene072756.t1 MTR_5g074320 31.862 725 457 15 68 778 10 711 1.92e-107 346
MS.gene072756.t1 MTR_3g037260 29.514 803 531 16 22 801 20 810 1.64e-103 337
MS.gene072756.t1 MTR_3g031160 28.553 788 519 17 10 781 23 782 4.49e-102 333
MS.gene072756.t1 MTR_6g015940 30.212 801 499 20 4 778 19 785 3.06e-97 320
MS.gene072756.t1 MTR_8g085260 29.032 806 510 19 1 778 18 789 1.73e-94 313
MS.gene072756.t1 MTR_4g037755 28.965 763 504 18 49 790 35 780 4.49e-93 309
MS.gene072756.t1 MTR_3g498715 28.394 803 492 20 9 774 32 788 8.55e-93 308
MS.gene072756.t1 MTR_4g029520 27.886 771 514 17 38 781 25 780 2.11e-92 307
MS.gene072756.t1 MTR_6g053320 29.877 733 457 20 81 785 69 772 5.76e-92 306
MS.gene072756.t1 MTR_5g088470 28.094 808 512 23 4 778 17 788 1.28e-91 305
MS.gene072756.t1 MTR_5g088450 28.464 801 521 18 4 778 17 791 7.30e-90 301
MS.gene072756.t1 MTR_3g110700 27.154 766 526 13 38 781 22 777 3.25e-88 296
MS.gene072756.t1 MTR_3g083850 27.715 801 520 18 4 778 17 784 4.44e-88 296
MS.gene072756.t1 MTR_5g088430 27.995 793 511 19 14 778 28 788 1.68e-86 291
MS.gene072756.t1 MTR_7g085870 27.456 794 503 20 25 778 34 794 1.66e-85 290
MS.gene072756.t1 MTR_3g031030 27.865 768 482 19 49 780 35 766 2.94e-83 282
MS.gene072756.t1 MTR_5g088790 27.318 798 530 15 9 778 39 814 2.08e-78 270
MS.gene072756.t1 MTR_7g053000 26.600 797 537 15 9 778 39 814 7.97e-77 266
MS.gene072756.t1 MTR_7g052680 27.091 801 528 18 9 778 39 814 9.55e-77 265
MS.gene072756.t1 MTR_5g088840 27.702 805 519 20 9 778 39 815 1.41e-76 265
MS.gene072756.t1 MTR_7g052970 26.600 797 537 15 9 778 39 814 8.79e-76 263
MS.gene072756.t1 MTR_3g465680 28.329 413 286 4 20 426 44 452 1.63e-50 191
MS.gene072756.t1 MTR_3g465680 30.052 386 237 12 401 778 555 915 3.24e-39 157
MS.gene072756.t1 MTR_7g052895 23.400 547 391 10 9 534 39 578 1.37e-40 160
MS.gene072756.t1 MTR_5g083560 33.333 342 187 12 469 796 1 315 1.55e-37 144
MS.gene072756.t1 MTR_5g061460 56.250 96 42 0 455 550 15 110 2.96e-31 120
MS.gene072756.t1 MTR_1g082340 56.250 96 42 0 455 550 82 177 6.22e-31 122
MS.gene072756.t1 MTR_3g083860 25.000 220 128 6 14 228 28 215 2.25e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072756.t1 AT5G41610 66.834 796 247 8 1 785 1 790 0.0 1047
MS.gene072756.t1 AT5G41610 66.834 796 247 8 1 785 1 790 0.0 1047
MS.gene072756.t1 AT4G23700 61.200 817 297 7 6 802 3 819 0.0 972
MS.gene072756.t1 AT4G23700 61.200 817 297 7 6 802 3 819 0.0 972
MS.gene072756.t1 AT5G41610 66.804 726 226 7 71 785 1 722 0.0 946
MS.gene072756.t1 AT3G17630 60.618 777 300 3 3 779 2 772 0.0 934
MS.gene072756.t1 AT3G17630 60.618 777 300 3 3 779 2 772 0.0 934
MS.gene072756.t1 AT1G64170 56.439 792 309 15 11 779 14 792 0.0 818
MS.gene072756.t1 AT2G13620 47.619 798 380 10 4 779 8 789 0.0 712
MS.gene072756.t1 AT3G53720 50.493 812 355 14 15 781 8 817 0.0 689
MS.gene072756.t1 AT3G53720 48.859 745 334 14 82 781 2 744 0.0 595
MS.gene072756.t1 AT1G05580 34.456 772 478 12 23 779 41 799 1.36e-135 424
MS.gene072756.t1 AT2G31910 33.290 769 491 10 24 779 28 787 7.00e-124 392
MS.gene072756.t1 AT1G05580 34.714 700 429 12 95 779 2 688 8.23e-123 387
MS.gene072756.t1 AT1G06970 31.242 781 502 15 10 778 27 784 7.01e-112 360
MS.gene072756.t1 AT2G31910 33.238 698 444 10 95 779 2 690 2.82e-109 351
MS.gene072756.t1 AT2G31910 33.238 698 444 10 95 779 2 690 2.82e-109 351
MS.gene072756.t1 AT2G30240 30.100 804 528 15 10 800 29 811 2.45e-103 338
MS.gene072756.t1 AT5G58460 27.861 804 527 15 10 778 42 827 1.12e-91 307
MS.gene072756.t1 AT5G37060 27.582 794 522 16 21 778 53 829 2.74e-91 306
MS.gene072756.t1 AT5G22900 27.206 816 518 19 14 778 27 817 2.56e-87 294
MS.gene072756.t1 AT5G01690 26.811 787 523 16 10 778 16 767 1.17e-82 280
MS.gene072756.t1 AT3G44900 27.518 814 524 20 14 783 26 817 2.82e-81 278
MS.gene072756.t1 AT2G28180 27.936 809 504 20 12 778 37 808 2.27e-77 267
MS.gene072756.t1 AT3G52080 26.265 830 511 21 3 778 2 784 4.87e-77 266
MS.gene072756.t1 AT1G08150 28.153 785 490 22 24 772 56 802 9.81e-77 265
MS.gene072756.t1 AT1G08140 27.696 751 484 22 57 778 83 803 1.19e-72 254
MS.gene072756.t1 AT5G01680 26.438 817 516 23 1 778 5 775 1.34e-72 253
MS.gene072756.t1 AT1G79400 27.132 774 533 16 24 778 11 772 2.70e-70 247
MS.gene072756.t1 AT2G28170 26.948 783 518 22 19 778 47 798 6.36e-69 243
MS.gene072756.t1 AT3G44930 24.967 769 513 15 31 769 35 769 2.83e-65 233
MS.gene072756.t1 AT1G16380 25.158 791 525 21 38 790 24 785 3.72e-65 233
MS.gene072756.t1 AT5G22910 27.013 770 499 18 31 768 47 785 1.80e-64 231
MS.gene072756.t1 AT3G44910 25.662 604 408 10 175 769 186 757 1.02e-61 223
MS.gene072756.t1 AT3G44920 25.161 775 518 16 31 776 35 776 9.42e-60 217
MS.gene072756.t1 AT1G08135 25.175 715 457 21 95 778 127 794 1.92e-51 193

Find 187 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATCTTCTAAAACCTTTA+AGG 0.099756 5.3:+3615932 MS.gene072756:CDS
TTCCGCATTTCTTGTATATA+TGG 0.132317 5.3:+3616767 MS.gene072756:CDS
GGTGAATGTTCCTCCATTTC+TGG 0.139824 5.3:-3618751 None:intergenic
ACCTCTCACAAAATTCTTTA+AGG 0.140574 5.3:-3617856 None:intergenic
TGATCAGACCTTTGCTATTA+TGG 0.150282 5.3:+3617598 MS.gene072756:CDS
GCTCTTGCTGTTGCCTTATC+AGG 0.189626 5.3:+3616924 MS.gene072756:CDS
GACAAAATGGGTTGCCGTTT+TGG 0.198490 5.3:+3617909 MS.gene072756:CDS
TCCTGTATTAGCCCGTATTT+TGG 0.201280 5.3:+3616815 MS.gene072756:CDS
ATGACTCGAGGTTGCCTTAA+AGG 0.205612 5.3:-3615946 None:intergenic
GTGATCTGTCCTACAATATT+AGG 0.206047 5.3:-3618379 None:intergenic
GTTACATTTACGGCTTGTTT+TGG 0.223359 5.3:+3617338 MS.gene072756:CDS
GCACAAAAGGTAAGACAAAA+TGG 0.243930 5.3:+3617896 MS.gene072756:CDS
ATTTCTGGGACAAAGTTTAT+TGG 0.255303 5.3:-3618736 None:intergenic
CCGGCAAACGCTCCATCTTT+TGG 0.270031 5.3:-3617188 None:intergenic
TTAGCTGCTGTTCTGGCTGC+TGG 0.279693 5.3:+3617101 MS.gene072756:CDS
TGGGCCATCCATTTGAAAAT+TGG 0.287723 5.3:-3617029 None:intergenic
TGCTACATTAGCTGCTGTTC+TGG 0.289882 5.3:+3617094 MS.gene072756:CDS
CCGCACAACAGCATCTGTTT+TGG 0.302658 5.3:+3618687 MS.gene072756:CDS
CCGATGCAGCCTGCGTCTAA+TGG 0.304884 5.3:+3615820 MS.gene072756:CDS
CCTAAATCTCTGCGGAAAAC+TGG 0.313847 5.3:+3616639 MS.gene072756:CDS
AGAAGCGAGTGCGCGGAATT+AGG 0.315072 5.3:+3618631 MS.gene072756:CDS
GACCAACATAGCTACCATTC+AGG 0.327011 5.3:+3617286 MS.gene072756:CDS
GACCGTGAAGCTCTTGCTTA+TGG 0.329784 5.3:+3618298 MS.gene072756:CDS
GTCCTTGCCATTGCTATTGC+CGG 0.332317 5.3:+3616669 MS.gene072756:CDS
TCCGCATTTCTTGTATATAT+GGG 0.333877 5.3:+3616768 MS.gene072756:CDS
CCATTCAGGGGCTGCAGTCA+TGG 0.338451 5.3:+3617300 MS.gene072756:CDS
TTACCCTTTGCACTAGGAAT+TGG 0.338535 5.3:+3616699 MS.gene072756:CDS
AACATTTCTAGCTGTGGTAT+TGG 0.344900 5.3:-3615788 None:intergenic
ATCGGAATTCATGCCATGTT+TGG 0.350615 5.3:+3617140 MS.gene072756:CDS
TTATTGGAACCTAATATTGT+AGG 0.354215 5.3:+3618370 MS.gene072756:CDS
AACCTTTGTTGCTTATGAAA+TGG 0.354534 5.3:-3618100 None:intergenic
TTCTGCAACTTATTTCTTGT+GGG 0.359669 5.3:+3618571 MS.gene072756:CDS
TGGTTCATCGTTTGTTCTAC+AGG 0.363937 5.3:+3616719 MS.gene072756:CDS
TGCGGAAGTATTTACACCTT+TGG 0.366289 5.3:-3616751 None:intergenic
GCTCACCTTCATGGCATTGT+TGG 0.366554 5.3:-3617049 None:intergenic
AAAATAGAGGATCTTGTATC+TGG 0.375448 5.3:+3617224 MS.gene072756:CDS
CTCACCTTCATGGCATTGTT+GGG 0.377633 5.3:-3617048 None:intergenic
ATCTCCATTTGTGTCATTGT+GGG 0.381610 5.3:+3616956 MS.gene072756:CDS
CATCAACCGGCTCACCTTCA+TGG 0.381935 5.3:-3617058 None:intergenic
CATTCAGGGGCTGCAGTCAT+GGG 0.383910 5.3:+3617301 MS.gene072756:CDS
ATTGATGAAATTGCTATCAT+TGG 0.392049 5.3:-3618013 None:intergenic
CCAAAAGATGGAGCGTTTGC+CGG 0.392899 5.3:+3617188 MS.gene072756:CDS
TAATCTTATGCCAGGGTGTT+CGG 0.399773 5.3:-3618332 None:intergenic
AATAAACTTTGTCCCAGAAA+TGG 0.400832 5.3:+3618738 MS.gene072756:CDS
AGGATTCTTGCCTGTTTCCA+TGG 0.404187 5.3:+3617746 MS.gene072756:CDS
GAAAAGCACAACAATGCAAT+AGG 0.404326 5.3:-3618260 None:intergenic
ATCTAATACTGGAAGACTCT+TGG 0.407831 5.3:-3616559 None:intergenic
TCGAAGGCCGGACGGTGAAT+TGG 0.408471 5.3:+3618594 MS.gene072756:CDS
CCATGTTATTGTGGCGTTCG+AGG 0.411384 5.3:+3617958 MS.gene072756:CDS
TTGCCTGTTTCCATGGTTCA+AGG 0.413150 5.3:+3617753 MS.gene072756:CDS
TTTCTGCAACTTATTTCTTG+TGG 0.414228 5.3:+3618570 MS.gene072756:CDS
GTGAATGTTCCTCCATTTCT+GGG 0.415037 5.3:-3618750 None:intergenic
TGCAAAGGGTAAGCTTATTC+CGG 0.415247 5.3:-3616688 None:intergenic
CTACTTGGGCCGTCAGCGCT+TGG 0.415516 5.3:+3616501 MS.gene072756:CDS
ATTCCTTGAACCATGGAAAC+AGG 0.420826 5.3:-3617756 None:intergenic
TTTGCTAGAGTATCTAATAC+TGG 0.434840 5.3:-3616570 None:intergenic
TTGTGTTAGCTGCGTATAAG+AGG 0.439955 5.3:+3617660 MS.gene072756:CDS
TCTAATACTGGAAGACTCTT+GGG 0.445615 5.3:-3616558 None:intergenic
CCGCATTTCTTGTATATATG+GGG 0.447740 5.3:+3616769 MS.gene072756:CDS
GCCAAAATACGGGCTAATAC+AGG 0.448964 5.3:-3616816 None:intergenic
ATTTCTTGTGGGTCGAAGGC+CGG 0.449582 5.3:+3618582 MS.gene072756:CDS
ATCGATACTAATGGCACAAA+AGG 0.453823 5.3:+3617883 MS.gene072756:CDS
GCGTCTAATGGTGTATTTCA+AGG 0.458325 5.3:+3615832 MS.gene072756:CDS
ACACCCTGGCATAAGATTAG+TGG 0.462991 5.3:+3618336 MS.gene072756:CDS
TAGCTGCTGTTCTGGCTGCT+GGG 0.463429 5.3:+3617102 MS.gene072756:CDS
CCCCTGAATGGTAGCTATGT+TGG 0.463640 5.3:-3617288 None:intergenic
TTTGATGAATAGTAAAGGCT+TGG 0.466519 5.3:+3617433 MS.gene072756:CDS
TCATTGTGGGTCTTCTTAAG+TGG 0.468755 5.3:+3616969 MS.gene072756:CDS
ATCGATGATAAACTTGATTG+AGG 0.470338 5.3:+3617784 MS.gene072756:CDS
TTGAACGAGTCTTTAGTGCT+TGG 0.470752 5.3:+3617407 MS.gene072756:CDS
GACGCAGGCTGCATCGGCGG+AGG 0.471752 5.3:-3615814 None:intergenic
TCTTGCAGTCACAAGGGGAC+TGG 0.473223 5.3:+3615909 MS.gene072756:CDS
TTTGTTGATCGCGGGCTTGG+TGG 0.473988 5.3:+3618208 MS.gene072756:CDS
AAGACCCACAATGACACAAA+TGG 0.479162 5.3:-3616960 None:intergenic
TGACAGGGAGGAATCCTACT+TGG 0.484231 5.3:+3616486 MS.gene072756:intron
AATGATAGCAATTTCATCAA+TGG 0.488394 5.3:+3618015 MS.gene072756:CDS
AGGACCTGTTGGTGGCTTGT+TGG 0.488446 5.3:+3618651 MS.gene072756:CDS
GTCTTGTTCTTGCAGTCACA+AGG 0.489013 5.3:+3615902 MS.gene072756:CDS
GAAGATTCCGTGTCTCCATC+AGG 0.489657 5.3:-3618772 None:intergenic
CATATGTATAACTCATCAAC+CGG 0.492318 5.3:-3617071 None:intergenic
AGATTGGCTATTGCCTGATA+AGG 0.492432 5.3:-3616937 None:intergenic
CAACATCGGTAAAGATAGAA+AGG 0.492937 5.3:+3617472 MS.gene072756:CDS
CTCCATAAGCAAGAGCTTCA+CGG 0.493215 5.3:-3618300 None:intergenic
CCATGACTGCAGCCCCTGAA+TGG 0.495906 5.3:-3617300 None:intergenic
CCACTTGACACAAAATAAAG+TGG 0.501014 5.3:-3617257 None:intergenic
AGAGTCTCCAACATCAACTT+TGG 0.501856 5.3:-3618401 None:intergenic
GCAAAGGCCAATTCACCGTC+CGG 0.504951 5.3:-3618601 None:intergenic
GATGCCAACAAGCCACCAAC+AGG 0.505963 5.3:-3618655 None:intergenic
AATCTCCATTTGTGTCATTG+TGG 0.506174 5.3:+3616955 MS.gene072756:CDS
TGGGTCTTCTTAAGTGGGTG+TGG 0.508408 5.3:+3616975 MS.gene072756:CDS
GAACCAATTCCTAGTGCAAA+GGG 0.508620 5.3:-3616702 None:intergenic
TTCTATCTTTACCGATGTTG+AGG 0.509100 5.3:-3617469 None:intergenic
TCAATCAAGTTTATCATCGA+TGG 0.509658 5.3:-3617782 None:intergenic
ACTTATTTCTTGTGGGTCGA+AGG 0.511823 5.3:+3618578 MS.gene072756:CDS
CATAAGCAACAAAGGTTAGA+CGG 0.512409 5.3:+3618106 MS.gene072756:CDS
TCTTGATTATGCACTTCCAT+TGG 0.513464 5.3:+3615864 MS.gene072756:CDS
AAAACCACTAATCTTATGCC+AGG 0.514861 5.3:-3618340 None:intergenic
CATTGTGGGTCTTCTTAAGT+GGG 0.515396 5.3:+3616970 MS.gene072756:CDS
AGCAAGAGCAAGAAGAATCC+AGG 0.517879 5.3:-3616910 None:intergenic
CACAAAAGGTAAGACAAAAT+GGG 0.518007 5.3:+3617897 MS.gene072756:CDS
TTATTCCGTCCAAGCGCTGA+CGG 0.519020 5.3:-3616510 None:intergenic
CTCGCTTCTTTCAAGAGCAA+AGG 0.521580 5.3:-3618617 None:intergenic
ACAATGACACAAATGGAGAT+TGG 0.521930 5.3:-3616953 None:intergenic
CCATTAGACGCAGGCTGCAT+CGG 0.522930 5.3:-3615820 None:intergenic
TCTTGCTTATGGAGCTCGTA+TGG 0.526592 5.3:+3618309 MS.gene072756:CDS
TTCCATTTCATAAGCAACAA+AGG 0.530602 5.3:+3618098 MS.gene072756:CDS
TTGGGCCGTCAGCGCTTGGA+CGG 0.534153 5.3:+3616505 MS.gene072756:CDS
GGGTTTGTCACGGATGCTAT+CGG 0.536342 5.3:+3617122 MS.gene072756:CDS
ATAAGCTTACCCTTTGCACT+AGG 0.536559 5.3:+3616693 MS.gene072756:CDS
ATAGATAATTGAGTCATCAC+TGG 0.537461 5.3:-3618491 None:intergenic
CGCATTTCTTGTATATATGG+GGG 0.537612 5.3:+3616770 MS.gene072756:CDS
TCTGGCTGCTGGGTTTGTCA+CGG 0.539409 5.3:+3617112 MS.gene072756:CDS
ACCAACATAGCTACCATTCA+GGG 0.542167 5.3:+3617287 MS.gene072756:CDS
CAGATCACCAAAGTTGATGT+TGG 0.543622 5.3:+3618394 MS.gene072756:CDS
ATGGAATATTCCTTGAACCA+TGG 0.546940 5.3:-3617763 None:intergenic
TGATGCAACAGTATCACAAT+GGG 0.550837 5.3:+3618710 MS.gene072756:CDS
TGGTGACCTTTATCCCAAAA+CGG 0.553372 5.3:-3617923 None:intergenic
TGCTCTTGTGGAGAAAATAG+AGG 0.555546 5.3:+3617211 MS.gene072756:CDS
GAGCATGCACCATGCTCAGT+TGG 0.559655 5.3:+3618181 MS.gene072756:CDS
ACGCAGGCTGCATCGGCGGA+GGG 0.563830 5.3:-3615813 None:intergenic
CAAGCGCTGACGGCCCAAGT+AGG 0.564029 5.3:-3616500 None:intergenic
CAGTTAATGATGTAGCAGCC+TGG 0.564394 5.3:+3616892 MS.gene072756:CDS
ATGGAGGAACATTCACCTGA+TGG 0.565713 5.3:+3618757 MS.gene072756:CDS
TGAAAATTGGAAGGACAATG+AGG 0.565770 5.3:-3617016 None:intergenic
AACCACTAGTGTCGGCAGAA+TGG 0.566373 5.3:+3616854 MS.gene072756:CDS
CACAAGAGGAGTGGTCATGA+AGG 0.566575 5.3:-3617642 None:intergenic
CATATCTGTAAAATAGCCAA+TGG 0.568781 5.3:-3615880 None:intergenic
CCATCCATTTGAAAATTGGA+AGG 0.571739 5.3:-3617025 None:intergenic
CCTCGAACGCCACAATAACA+TGG 0.571844 5.3:-3617958 None:intergenic
AGCACTAAAGACTCGTTCAA+AGG 0.572987 5.3:-3617404 None:intergenic
GCGGAATTAGGACCTGTTGG+TGG 0.575797 5.3:+3618643 MS.gene072756:CDS
CTTGTTCTTGCAGTCACAAG+GGG 0.579341 5.3:+3615904 MS.gene072756:CDS
TGCGCGGAATTAGGACCTGT+TGG 0.580143 5.3:+3618640 MS.gene072756:CDS
GCTCGTATGGCCGAACACCC+TGG 0.581362 5.3:+3618322 MS.gene072756:CDS
GGTTCATCGTTTGTTCTACA+GGG 0.584720 5.3:+3616720 MS.gene072756:CDS
ATTCAGGGGCTGCAGTCATG+GGG 0.586038 5.3:+3617302 MS.gene072756:CDS
ACAATGCCATGAAGGTGAGC+CGG 0.586955 5.3:+3617052 MS.gene072756:CDS
AGCGTTTGCCGGTGCTCTTG+TGG 0.587306 5.3:+3617199 MS.gene072756:CDS
CATGAAAACCATAATAGCAA+AGG 0.589901 5.3:-3617606 None:intergenic
GAATTAATTGTCCTCAACAT+CGG 0.591363 5.3:+3617458 MS.gene072756:CDS
GAGGTCCTCATCGATACTAA+TGG 0.592793 5.3:+3617874 MS.gene072756:CDS
TCTTGTTCTTGCAGTCACAA+GGG 0.592967 5.3:+3615903 MS.gene072756:CDS
GTGATGCAACAGTATCACAA+TGG 0.594247 5.3:+3618709 MS.gene072756:CDS
AACAGGCAAGAATCCTCAGT+TGG 0.594260 5.3:-3617739 None:intergenic
TTAGACGCAGGCTGCATCGG+CGG 0.596165 5.3:-3615817 None:intergenic
TTAGATACTCTAGCAAACCT+TGG 0.598078 5.3:+3616576 MS.gene072756:CDS
TGAACCAATTCCTAGTGCAA+AGG 0.603458 5.3:-3616703 None:intergenic
ACAAATACAGAACAATTGAA+AGG 0.605182 5.3:+3617702 MS.gene072756:CDS
TTCCGGCAATAGCAATGGCA+AGG 0.606740 5.3:-3616671 None:intergenic
GCTTATTCCGGCAATAGCAA+TGG 0.606834 5.3:-3616676 None:intergenic
CTTGTGGGTCGAAGGCCGGA+CGG 0.607981 5.3:+3618586 MS.gene072756:CDS
AAACTTTGTCCCAGAAATGG+AGG 0.612453 5.3:+3618741 MS.gene072756:CDS
AACTTGATTGAGGCTTCAAG+AGG 0.612831 5.3:+3617794 MS.gene072756:CDS
GACAGGGAGGAATCCTACTT+GGG 0.614800 5.3:+3616487 MS.gene072756:intron
AATACTGGAAGACTCTTGGG+TGG 0.618474 5.3:-3616555 None:intergenic
AGATTCTGTCCATGTTATTG+TGG 0.620026 5.3:+3617949 MS.gene072756:CDS
AAACTTCTAACCACTAGTGT+CGG 0.620589 5.3:+3616846 MS.gene072756:CDS
ATGGCCCAACAATGCCATGA+AGG 0.621341 5.3:+3617044 MS.gene072756:CDS
TAACATGGACAGAATCTCCA+TGG 0.622510 5.3:-3617943 None:intergenic
GCAGAAATTAATCACGCGGA+TGG 0.623209 5.3:-3618554 None:intergenic
AGGGCAAACATTTCTAGCTG+TGG 0.625060 5.3:-3615794 None:intergenic
CACTTGACACAAAATAAAGT+GGG 0.625867 5.3:-3617256 None:intergenic
TTATACGCAGCTAACACAAG+AGG 0.626543 5.3:-3617656 None:intergenic
TTGTGCCATTAGTATCGATG+AGG 0.638116 5.3:-3617879 None:intergenic
AAGAGGAGTGGTCATGAAGG+TGG 0.640702 5.3:-3617639 None:intergenic
CCAAAACAGATGCTGTTGTG+CGG 0.640724 5.3:-3618687 None:intergenic
AAAATGCAGACGGCCAACTG+AGG 0.641897 5.3:+3617726 MS.gene072756:CDS
AATGGAAGTGCATAATCAAG+AGG 0.642363 5.3:-3615862 None:intergenic
AGCCATTCTGCCGACACTAG+TGG 0.642910 5.3:-3616856 None:intergenic
AAATCTCTGCGGAAAACTGG+AGG 0.646572 5.3:+3616642 MS.gene072756:CDS
CGCAGCTAACACAAGAGGAG+TGG 0.647065 5.3:-3617651 None:intergenic
ATACTGGAAGACTCTTGGGT+GGG 0.647325 5.3:-3616554 None:intergenic
AAACCACTAATCTTATGCCA+GGG 0.648115 5.3:-3618339 None:intergenic
TCTTGAAAGAAGCGAGTGCG+CGG 0.650120 5.3:+3618624 MS.gene072756:CDS
ACATTCACCTGATGGAGACA+CGG 0.653891 5.3:+3618765 MS.gene072756:CDS
ACAATCTCTGCAATGACTCG+AGG 0.658940 5.3:-3615958 None:intergenic
CTACAGGGAACAATTGCCAA+AGG 0.662191 5.3:+3616735 MS.gene072756:CDS
ATGGAAGTGCATAATCAAGA+GGG 0.669234 5.3:-3615861 None:intergenic
ATAGAAAGAATATAAGACCA+AGG 0.670462 5.3:-3616593 None:intergenic
TGAAATACACCATTAGACGC+AGG 0.678423 5.3:-3615829 None:intergenic
CCCCATATATACAAGAAATG+CGG 0.682370 5.3:-3616769 None:intergenic
AGTTGCAGAAATTAATCACG+CGG 0.687377 5.3:-3618558 None:intergenic
TCATTGGTCGAACACACACG+TGG 0.711445 5.3:-3617997 None:intergenic
AGTTAGATCCTAAATCTCTG+CGG 0.716660 5.3:+3616631 MS.gene072756:CDS
CCAACATAGCTACCATTCAG+GGG 0.726005 5.3:+3617288 MS.gene072756:CDS
AAATGGAAGAATGATGACAG+CGG 0.728068 5.3:-3618083 None:intergenic
GATGCAACAGTATCACAATG+GGG 0.828892 5.3:+3618711 MS.gene072756:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCCTATAATTAATTTAAAA+TGG + chr5.3:3617525-3617544 MS.gene072756:intron 10.0%
!! AATTAATTATAGGAATAATG+AGG - chr5.3:3617520-3617539 None:intergenic 15.0%
!! ACTGTGAAATTATATAAAAA+TGG - chr5.3:3616137-3616156 None:intergenic 15.0%
!! TTTATATATTAGAAACTAGT+GGG + chr5.3:3616071-3616090 MS.gene072756:intron 15.0%
!!! CACCATTTTAAATTAATTAT+AGG - chr5.3:3617530-3617549 None:intergenic 15.0%
!!! TTTTATATATTAGAAACTAG+TGG + chr5.3:3616070-3616089 MS.gene072756:intron 15.0%
!! TTATATATTAGAAACTAGTG+GGG + chr5.3:3616072-3616091 MS.gene072756:intron 20.0%
!!! ATATTCTTTCTATTTTTAGC+AGG + chr5.3:3616603-3616622 MS.gene072756:CDS 20.0%
! AACTTCATTAAATCCACATT+TGG + chr5.3:3616378-3616397 MS.gene072756:intron 25.0%
! AATGATAGCAATTTCATCAA+TGG + chr5.3:3618015-3618034 MS.gene072756:CDS 25.0%
! ACAAATACAGAACAATTGAA+AGG + chr5.3:3617702-3617721 MS.gene072756:CDS 25.0%
! ACCAAAATTCCAAAAACAAA+CGG - chr5.3:3617167-3617186 None:intergenic 25.0%
! ATAGAAAGAATATAAGACCA+AGG - chr5.3:3616596-3616615 None:intergenic 25.0%
! ATATAAAAATGGAACACTCA+AGG - chr5.3:3616126-3616145 None:intergenic 25.0%
! ATTGATGAAATTGCTATCAT+TGG - chr5.3:3618016-3618035 None:intergenic 25.0%
! TTATTGGAACCTAATATTGT+AGG + chr5.3:3618370-3618389 MS.gene072756:CDS 25.0%
!! GGATTTTTGATGAATAGTAA+AGG + chr5.3:3617428-3617447 MS.gene072756:CDS 25.0%
!! TATAAGAAACGATTTTCGAT+TGG + chr5.3:3618141-3618160 MS.gene072756:CDS 25.0%
!! TTAGAACTTGTTTCATAGTT+TGG - chr5.3:3616187-3616206 None:intergenic 25.0%
!!! ATTTTCGATTGGTTAACAAA+AGG + chr5.3:3618152-3618171 MS.gene072756:CDS 25.0%
!!! TCTAGTTTTCGTTACATTTA+CGG + chr5.3:3617328-3617347 MS.gene072756:CDS 25.0%
!!! TTTTCGATTGGTTAACAAAA+GGG + chr5.3:3618153-3618172 MS.gene072756:CDS 25.0%
AAAATAGAGGATCTTGTATC+TGG + chr5.3:3617224-3617243 MS.gene072756:CDS 30.0%
AATAAACTTTGTCCCAGAAA+TGG + chr5.3:3618738-3618757 MS.gene072756:CDS 30.0%
ACCTCTCACAAAATTCTTTA+AGG - chr5.3:3617859-3617878 None:intergenic 30.0%
ATAGATAATTGAGTCATCAC+TGG - chr5.3:3618494-3618513 None:intergenic 30.0%
ATCGATGATAAACTTGATTG+AGG + chr5.3:3617784-3617803 MS.gene072756:CDS 30.0%
ATTGAAAGGAAAAATGCAGA+CGG + chr5.3:3617716-3617735 MS.gene072756:CDS 30.0%
CAAATATACATCACGATGAT+CGG - chr5.3:3617554-3617573 None:intergenic 30.0%
CACAAAAGGTAAGACAAAAT+GGG + chr5.3:3617897-3617916 MS.gene072756:CDS 30.0%
CACTTGACACAAAATAAAGT+GGG - chr5.3:3617259-3617278 None:intergenic 30.0%
CATATCTGTAAAATAGCCAA+TGG - chr5.3:3615883-3615902 None:intergenic 30.0%
CATATGTATAACTCATCAAC+CGG - chr5.3:3617074-3617093 None:intergenic 30.0%
CATGAAAACCATAATAGCAA+AGG - chr5.3:3617609-3617628 None:intergenic 30.0%
CCAAAATTCCAAAAACAAAC+GGG - chr5.3:3617166-3617185 None:intergenic 30.0%
GAATTAATTGTCCTCAACAT+CGG + chr5.3:3617458-3617477 MS.gene072756:CDS 30.0%
TCAATCAAGTTTATCATCGA+TGG - chr5.3:3617785-3617804 None:intergenic 30.0%
TCCGCATTTCTTGTATATAT+GGG + chr5.3:3616768-3616787 MS.gene072756:CDS 30.0%
TTCCATTTCATAAGCAACAA+AGG + chr5.3:3618098-3618117 MS.gene072756:CDS 30.0%
TTCCGCATTTCTTGTATATA+TGG + chr5.3:3616767-3616786 MS.gene072756:CDS 30.0%
TTTGATGAATAGTAAAGGCT+TGG + chr5.3:3617433-3617452 MS.gene072756:CDS 30.0%
! AACCTTTGTTGCTTATGAAA+TGG - chr5.3:3618103-3618122 None:intergenic 30.0%
! ACCTTAAAGAATTTTGTGAG+AGG + chr5.3:3617855-3617874 MS.gene072756:CDS 30.0%
! TATTTGATTTACGTATCTGC+AGG + chr5.3:3617568-3617587 MS.gene072756:intron 30.0%
! TCTTAAACATGTGCACATTT+TGG - chr5.3:3616307-3616326 None:intergenic 30.0%
! TTCTGCAACTTATTTCTTGT+GGG + chr5.3:3618571-3618590 MS.gene072756:CDS 30.0%
! TTGTCCTTCCAATTTTCAAA+TGG + chr5.3:3617021-3617040 MS.gene072756:CDS 30.0%
! TTTCTGCAACTTATTTCTTG+TGG + chr5.3:3618570-3618589 MS.gene072756:CDS 30.0%
! TTTGCTAGAGTATCTAATAC+TGG - chr5.3:3616573-3616592 None:intergenic 30.0%
!! ATTGCTTATTGATGATGACA+GGG + chr5.3:3616471-3616490 MS.gene072756:intron 30.0%
!! ATTTCTGGGACAAAGTTTAT+TGG - chr5.3:3618739-3618758 None:intergenic 30.0%
!! CCTATCTTCTAAAACCTTTA+AGG + chr5.3:3615932-3615951 MS.gene072756:CDS 30.0%
!! TTACATTTACGGCTTGTTTT+GGG + chr5.3:3617339-3617358 MS.gene072756:CDS 30.0%
!!! AGTGGTTAGAAGTTTTAACT+CGG - chr5.3:3616841-3616860 None:intergenic 30.0%
!!! CCTTAAAGGTTTTAGAAGAT+AGG - chr5.3:3615935-3615954 None:intergenic 30.0%
!!! CTTTGCTATTATGGTTTTCA+TGG + chr5.3:3617607-3617626 MS.gene072756:CDS 30.0%
AAAACCACTAATCTTATGCC+AGG - chr5.3:3618343-3618362 None:intergenic 35.0%
AAACCACTAATCTTATGCCA+GGG - chr5.3:3618342-3618361 None:intergenic 35.0%
AAACTTCTAACCACTAGTGT+CGG + chr5.3:3616846-3616865 MS.gene072756:CDS 35.0%
AAATGGAAGAATGATGACAG+CGG - chr5.3:3618086-3618105 None:intergenic 35.0%
ACAAAAATTCCAACTGAGCA+TGG - chr5.3:3618193-3618212 None:intergenic 35.0%
ACAATGACACAAATGGAGAT+TGG - chr5.3:3616956-3616975 None:intergenic 35.0%
AGATTCTGTCCATGTTATTG+TGG + chr5.3:3617949-3617968 MS.gene072756:CDS 35.0%
AGTTAGATCCTAAATCTCTG+CGG + chr5.3:3616631-3616650 MS.gene072756:CDS 35.0%
AGTTGCAGAAATTAATCACG+CGG - chr5.3:3618561-3618580 None:intergenic 35.0%
ATCGATACTAATGGCACAAA+AGG + chr5.3:3617883-3617902 MS.gene072756:CDS 35.0%
ATCTAATACTGGAAGACTCT+TGG - chr5.3:3616562-3616581 None:intergenic 35.0%
ATGGAATATTCCTTGAACCA+TGG - chr5.3:3617766-3617785 None:intergenic 35.0%
CAACATCGGTAAAGATAGAA+AGG + chr5.3:3617472-3617491 MS.gene072756:CDS 35.0%
CATAAGCAACAAAGGTTAGA+CGG + chr5.3:3618106-3618125 MS.gene072756:CDS 35.0%
CCACTTGACACAAAATAAAG+TGG - chr5.3:3617260-3617279 None:intergenic 35.0%
CCATCCATTTGAAAATTGGA+AGG - chr5.3:3617028-3617047 None:intergenic 35.0%
CCCCATATATACAAGAAATG+CGG - chr5.3:3616772-3616791 None:intergenic 35.0%
CCGCATTTCTTGTATATATG+GGG + chr5.3:3616769-3616788 MS.gene072756:CDS 35.0%
CGCATTTCTTGTATATATGG+GGG + chr5.3:3616770-3616789 MS.gene072756:CDS 35.0%
GCACAAAAGGTAAGACAAAA+TGG + chr5.3:3617896-3617915 MS.gene072756:CDS 35.0%
GTGATCTGTCCTACAATATT+AGG - chr5.3:3618382-3618401 None:intergenic 35.0%
TCTAATACTGGAAGACTCTT+GGG - chr5.3:3616561-3616580 None:intergenic 35.0%
TCTTGATTATGCACTTCCAT+TGG + chr5.3:3615864-3615883 MS.gene072756:CDS 35.0%
TGAAAATTGGAAGGACAATG+AGG - chr5.3:3617019-3617038 None:intergenic 35.0%
TGATGCAACAGTATCACAAT+GGG + chr5.3:3618710-3618729 MS.gene072756:CDS 35.0%
TTAGAAACTAGTGGGGAAAT+TGG + chr5.3:3616079-3616098 MS.gene072756:intron 35.0%
TTAGATACTCTAGCAAACCT+TGG + chr5.3:3616576-3616595 MS.gene072756:CDS 35.0%
TTAGTAAGACTGACCAAATG+TGG - chr5.3:3616394-3616413 None:intergenic 35.0%
TTCTATCTTTACCGATGTTG+AGG - chr5.3:3617472-3617491 None:intergenic 35.0%
! AATCTCCATTTGTGTCATTG+TGG + chr5.3:3616955-3616974 MS.gene072756:CDS 35.0%
! AATGGAAGTGCATAATCAAG+AGG - chr5.3:3615865-3615884 None:intergenic 35.0%
! AGTGGTTTTTCGCTTCTTAT+TGG + chr5.3:3618354-3618373 MS.gene072756:CDS 35.0%
! ATCTCCATTTGTGTCATTGT+GGG + chr5.3:3616956-3616975 MS.gene072756:CDS 35.0%
! ATGGAAGTGCATAATCAAGA+GGG - chr5.3:3615864-3615883 None:intergenic 35.0%
! ATTGTTGTGCTTTTCTTTGG+TGG + chr5.3:3618268-3618287 MS.gene072756:CDS 35.0%
! CCTTCCAATTTTCAAATGGA+TGG + chr5.3:3617025-3617044 MS.gene072756:CDS 35.0%
! GAAAAGCACAACAATGCAAT+AGG - chr5.3:3618263-3618282 None:intergenic 35.0%
! GTTGGAATTTTTGTTGATCG+CGG + chr5.3:3618199-3618218 MS.gene072756:CDS 35.0%
! TGCATTGTTGTGCTTTTCTT+TGG + chr5.3:3618265-3618284 MS.gene072756:CDS 35.0%
! TTGGAATTTTTGTTGATCGC+GGG + chr5.3:3618200-3618219 MS.gene072756:CDS 35.0%
!! AACATTTCTAGCTGTGGTAT+TGG - chr5.3:3615791-3615810 None:intergenic 35.0%
!! AACCTCTGCTCTATTTTAGA+AGG - chr5.3:3616420-3616439 None:intergenic 35.0%
!! GATTGCTTATTGATGATGAC+AGG + chr5.3:3616470-3616489 MS.gene072756:intron 35.0%
!! GTTACATTTACGGCTTGTTT+TGG + chr5.3:3617338-3617357 MS.gene072756:CDS 35.0%
!! TGATCAGACCTTTGCTATTA+TGG + chr5.3:3617598-3617617 MS.gene072756:CDS 35.0%
!! TTTTAACTCGGCCAAAATAC+GGG - chr5.3:3616829-3616848 None:intergenic 35.0%
!!! CCACTTTATTTTGTGTCAAG+TGG + chr5.3:3617257-3617276 MS.gene072756:CDS 35.0%
!!! CCCGTTTGTTTTTGGAATTT+TGG + chr5.3:3617163-3617182 MS.gene072756:CDS 35.0%
!!! CTTGTTTTGGGAAGATTGTT+GGG + chr5.3:3617351-3617370 MS.gene072756:CDS 35.0%
!!! GGAATTTTGGTTCCAAAAGA+TGG + chr5.3:3617176-3617195 MS.gene072756:CDS 35.0%
!!! GTTTTAACTCGGCCAAAATA+CGG - chr5.3:3616830-3616849 None:intergenic 35.0%
AAAAACAAACGGGCCAAACA+TGG - chr5.3:3617156-3617175 None:intergenic 40.0%
AAACTTTGTCCCAGAAATGG+AGG + chr5.3:3618741-3618760 MS.gene072756:CDS 40.0%
AACTTGATTGAGGCTTCAAG+AGG + chr5.3:3617794-3617813 MS.gene072756:CDS 40.0%
AAGACCCACAATGACACAAA+TGG - chr5.3:3616963-3616982 None:intergenic 40.0%
ACCAACATAGCTACCATTCA+GGG + chr5.3:3617287-3617306 MS.gene072756:CDS 40.0%
AGAGTCTCCAACATCAACTT+TGG - chr5.3:3618404-3618423 None:intergenic 40.0%
AGCACTAAAGACTCGTTCAA+AGG - chr5.3:3617407-3617426 None:intergenic 40.0%
ATAAGCTTACCCTTTGCACT+AGG + chr5.3:3616693-3616712 MS.gene072756:CDS 40.0%
ATCGGAATTCATGCCATGTT+TGG + chr5.3:3617140-3617159 MS.gene072756:CDS 40.0%
ATTCCTTGAACCATGGAAAC+AGG - chr5.3:3617759-3617778 None:intergenic 40.0%
CATTGTGGGTCTTCTTAAGT+GGG + chr5.3:3616970-3616989 MS.gene072756:CDS 40.0%
GAACCAATTCCTAGTGCAAA+GGG - chr5.3:3616705-3616724 None:intergenic 40.0%
GATGCAACAGTATCACAATG+GGG + chr5.3:3618711-3618730 MS.gene072756:CDS 40.0%
GCAGAGAGTTTAAACACACT+TGG + chr5.3:3616355-3616374 MS.gene072756:intron 40.0%
GCGTCTAATGGTGTATTTCA+AGG + chr5.3:3615832-3615851 MS.gene072756:CDS 40.0%
GGTTCATCGTTTGTTCTACA+GGG + chr5.3:3616720-3616739 MS.gene072756:CDS 40.0%
GTGAATGTTCCTCCATTTCT+GGG - chr5.3:3618753-3618772 None:intergenic 40.0%
GTGATGCAACAGTATCACAA+TGG + chr5.3:3618709-3618728 MS.gene072756:CDS 40.0%
TAACATGGACAGAATCTCCA+TGG - chr5.3:3617946-3617965 None:intergenic 40.0%
TCATTGTGGGTCTTCTTAAG+TGG + chr5.3:3616969-3616988 MS.gene072756:CDS 40.0%
TCTTGTTCTTGCAGTCACAA+GGG + chr5.3:3615903-3615922 MS.gene072756:CDS 40.0%
TGAAATACACCATTAGACGC+AGG - chr5.3:3615832-3615851 None:intergenic 40.0%
TGAACCAATTCCTAGTGCAA+AGG - chr5.3:3616706-3616725 None:intergenic 40.0%
TGCAAAGGGTAAGCTTATTC+CGG - chr5.3:3616691-3616710 None:intergenic 40.0%
TGCTCTTGTGGAGAAAATAG+AGG + chr5.3:3617211-3617230 MS.gene072756:CDS 40.0%
TGGGCCATCCATTTGAAAAT+TGG - chr5.3:3617032-3617051 None:intergenic 40.0%
TGGTGACCTTTATCCCAAAA+CGG - chr5.3:3617926-3617945 None:intergenic 40.0%
TGGTTCATCGTTTGTTCTAC+AGG + chr5.3:3616719-3616738 MS.gene072756:CDS 40.0%
TTACCCTTTGCACTAGGAAT+TGG + chr5.3:3616699-3616718 MS.gene072756:CDS 40.0%
TTATACGCAGCTAACACAAG+AGG - chr5.3:3617659-3617678 None:intergenic 40.0%
TTGAACGAGTCTTTAGTGCT+TGG + chr5.3:3617407-3617426 MS.gene072756:CDS 40.0%
TTGTGTTAGCTGCGTATAAG+AGG + chr5.3:3617660-3617679 MS.gene072756:CDS 40.0%
! ACTTATTTCTTGTGGGTCGA+AGG + chr5.3:3618578-3618597 MS.gene072756:CDS 40.0%
! AGATTGGCTATTGCCTGATA+AGG - chr5.3:3616940-3616959 None:intergenic 40.0%
! TAATCTTATGCCAGGGTGTT+CGG - chr5.3:3618335-3618354 None:intergenic 40.0%
! TCCTGTATTAGCCCGTATTT+TGG + chr5.3:3616815-3616834 MS.gene072756:CDS 40.0%
!! ATGTTTGGCCCGTTTGTTTT+TGG + chr5.3:3617155-3617174 MS.gene072756:CDS 40.0%
!! CAGATCACCAAAGTTGATGT+TGG + chr5.3:3618394-3618413 MS.gene072756:CDS 40.0%
!! TATTTTCTCCACAAGAGCAC+CGG - chr5.3:3617210-3617229 None:intergenic 40.0%
!! TGCCTTCTAAAATAGAGCAG+AGG + chr5.3:3616415-3616434 MS.gene072756:intron 40.0%
!! TGCGGAAGTATTTACACCTT+TGG - chr5.3:3616754-3616773 None:intergenic 40.0%
!! TTGTGCCATTAGTATCGATG+AGG - chr5.3:3617882-3617901 None:intergenic 40.0%
!!! ACAAAATGGGTTGCCGTTTT+GGG + chr5.3:3617910-3617929 MS.gene072756:CDS 40.0%
!!! GCTTGTTTTGGGAAGATTGT+TGG + chr5.3:3617350-3617369 MS.gene072756:CDS 40.0%
!!! TTTTGGGATAAAGGTCACCA+TGG + chr5.3:3617926-3617945 MS.gene072756:CDS 40.0%
AAATCTCTGCGGAAAACTGG+AGG + chr5.3:3616642-3616661 MS.gene072756:CDS 45.0%
AACAGGCAAGAATCCTCAGT+TGG - chr5.3:3617742-3617761 None:intergenic 45.0%
AATACTGGAAGACTCTTGGG+TGG - chr5.3:3616558-3616577 None:intergenic 45.0%
ACAATCTCTGCAATGACTCG+AGG - chr5.3:3615961-3615980 None:intergenic 45.0%
ACACCCTGGCATAAGATTAG+TGG + chr5.3:3618336-3618355 MS.gene072756:CDS 45.0%
ACATTCACCTGATGGAGACA+CGG + chr5.3:3618765-3618784 MS.gene072756:CDS 45.0%
AGCAAGAGCAAGAAGAATCC+AGG - chr5.3:3616913-3616932 None:intergenic 45.0%
AGGATTCTTGCCTGTTTCCA+TGG + chr5.3:3617746-3617765 MS.gene072756:CDS 45.0%
ATACTGGAAGACTCTTGGGT+GGG - chr5.3:3616557-3616576 None:intergenic 45.0%
ATGACTCGAGGTTGCCTTAA+AGG - chr5.3:3615949-3615968 None:intergenic 45.0%
ATGGAGGAACATTCACCTGA+TGG + chr5.3:3618757-3618776 MS.gene072756:CDS 45.0%
CAGTTAATGATGTAGCAGCC+TGG + chr5.3:3616892-3616911 MS.gene072756:CDS 45.0%
CCAAAACAGATGCTGTTGTG+CGG - chr5.3:3618690-3618709 None:intergenic 45.0%
CCAACATAGCTACCATTCAG+GGG + chr5.3:3617288-3617307 MS.gene072756:CDS 45.0%
CCTAAATCTCTGCGGAAAAC+TGG + chr5.3:3616639-3616658 MS.gene072756:CDS 45.0%
CTACAGGGAACAATTGCCAA+AGG + chr5.3:3616735-3616754 MS.gene072756:CDS 45.0%
CTCACCTTCATGGCATTGTT+GGG - chr5.3:3617051-3617070 None:intergenic 45.0%
CTCCATAAGCAAGAGCTTCA+CGG - chr5.3:3618303-3618322 None:intergenic 45.0%
CTCGCTTCTTTCAAGAGCAA+AGG - chr5.3:3618620-3618639 None:intergenic 45.0%
CTTGTTCTTGCAGTCACAAG+GGG + chr5.3:3615904-3615923 MS.gene072756:CDS 45.0%
GACCAACATAGCTACCATTC+AGG + chr5.3:3617286-3617305 MS.gene072756:CDS 45.0%
GAGGTCCTCATCGATACTAA+TGG + chr5.3:3617874-3617893 MS.gene072756:CDS 45.0%
GCAGAAATTAATCACGCGGA+TGG - chr5.3:3618557-3618576 None:intergenic 45.0%
GCTTATTCCGGCAATAGCAA+TGG - chr5.3:3616679-3616698 None:intergenic 45.0%
GGTGAATGTTCCTCCATTTC+TGG - chr5.3:3618754-3618773 None:intergenic 45.0%
TCTTGCTTATGGAGCTCGTA+TGG + chr5.3:3618309-3618328 MS.gene072756:CDS 45.0%
TGCTACATTAGCTGCTGTTC+TGG + chr5.3:3617094-3617113 MS.gene072756:CDS 45.0%
TTGCCTGTTTCCATGGTTCA+AGG + chr5.3:3617753-3617772 MS.gene072756:CDS 45.0%
! ATTTTTGTTGATCGCGGGCT+TGG + chr5.3:3618205-3618224 MS.gene072756:CDS 45.0%
! CCAGTTTTCCGCAGAGATTT+AGG - chr5.3:3616642-3616661 None:intergenic 45.0%
! GCCAAAATACGGGCTAATAC+AGG - chr5.3:3616819-3616838 None:intergenic 45.0%
! GTCTTGTTCTTGCAGTCACA+AGG + chr5.3:3615902-3615921 MS.gene072756:CDS 45.0%
! GTTGTGCTTTTCTTTGGTGG+TGG + chr5.3:3618271-3618290 MS.gene072756:CDS 45.0%
!! AGGGCAAACATTTCTAGCTG+TGG - chr5.3:3615797-3615816 None:intergenic 45.0%
!! CACACACGTGGCTTAGTTTT+TGG - chr5.3:3617988-3618007 None:intergenic 45.0%
!! GCTTATTGATGATGACAGGG+AGG + chr5.3:3616474-3616493 MS.gene072756:intron 45.0%
!!! GACAAAATGGGTTGCCGTTT+TGG + chr5.3:3617909-3617928 MS.gene072756:CDS 45.0%
!!! GTGGGTGTGGTTTTGTTGTA+TGG + chr5.3:3616988-3617007 MS.gene072756:CDS 45.0%
AAAATGCAGACGGCCAACTG+AGG + chr5.3:3617726-3617745 MS.gene072756:CDS 50.0%
AAGAGGAGTGGTCATGAAGG+TGG - chr5.3:3617642-3617661 None:intergenic 50.0%
ACAATGCCATGAAGGTGAGC+CGG + chr5.3:3617052-3617071 MS.gene072756:CDS 50.0%
ATGGCCCAACAATGCCATGA+AGG + chr5.3:3617044-3617063 MS.gene072756:CDS 50.0%
ATTTCTTGTGGGTCGAAGGC+CGG + chr5.3:3618582-3618601 MS.gene072756:CDS 50.0%
CACAAGAGGAGTGGTCATGA+AGG - chr5.3:3617645-3617664 None:intergenic 50.0%
CCATGTTATTGTGGCGTTCG+AGG + chr5.3:3617958-3617977 MS.gene072756:CDS 50.0%
CCCCTGAATGGTAGCTATGT+TGG - chr5.3:3617291-3617310 None:intergenic 50.0%
CCTCGAACGCCACAATAACA+TGG - chr5.3:3617961-3617980 None:intergenic 50.0%
GAAGATTCCGTGTCTCCATC+AGG - chr5.3:3618775-3618794 None:intergenic 50.0%
GACAGGGAGGAATCCTACTT+GGG + chr5.3:3616487-3616506 MS.gene072756:intron 50.0%
GACCGTGAAGCTCTTGCTTA+TGG + chr5.3:3618298-3618317 MS.gene072756:CDS 50.0%
GCTCACCTTCATGGCATTGT+TGG - chr5.3:3617052-3617071 None:intergenic 50.0%
GGGTTTGTCACGGATGCTAT+CGG + chr5.3:3617122-3617141 MS.gene072756:CDS 50.0%
TCATTGGTCGAACACACACG+TGG - chr5.3:3618000-3618019 None:intergenic 50.0%
TCTTGAAAGAAGCGAGTGCG+CGG + chr5.3:3618624-3618643 MS.gene072756:CDS 50.0%
TGACAGGGAGGAATCCTACT+TGG + chr5.3:3616486-3616505 MS.gene072756:intron 50.0%
TGGGTCTTCTTAAGTGGGTG+TGG + chr5.3:3616975-3616994 MS.gene072756:CDS 50.0%
TTCCGGCAATAGCAATGGCA+AGG - chr5.3:3616674-3616693 None:intergenic 50.0%
! CCAAAAGATGGAGCGTTTGC+CGG + chr5.3:3617188-3617207 MS.gene072756:CDS 50.0%
! CCGCACAACAGCATCTGTTT+TGG + chr5.3:3618687-3618706 MS.gene072756:CDS 50.0%
! GTCCTTGCCATTGCTATTGC+CGG + chr5.3:3616669-3616688 MS.gene072756:CDS 50.0%
!! AACCACTAGTGTCGGCAGAA+TGG + chr5.3:3616854-3616873 MS.gene072756:CDS 50.0%
!! GCTCTTGCTGTTGCCTTATC+AGG + chr5.3:3616924-3616943 MS.gene072756:CDS 50.0%
!! TTATTCCGTCCAAGCGCTGA+CGG - chr5.3:3616513-3616532 None:intergenic 50.0%
!!! GGGTTGCCGTTTTGGGATAA+AGG + chr5.3:3617917-3617936 MS.gene072756:CDS 50.0%
AGAAGCGAGTGCGCGGAATT+AGG + chr5.3:3618631-3618650 MS.gene072756:CDS 55.0%
AGCCATTCTGCCGACACTAG+TGG - chr5.3:3616859-3616878 None:intergenic 55.0%
ATTCAGGGGCTGCAGTCATG+GGG + chr5.3:3617302-3617321 MS.gene072756:CDS 55.0%
CATTCAGGGGCTGCAGTCAT+GGG + chr5.3:3617301-3617320 MS.gene072756:CDS 55.0%
CCATTAGACGCAGGCTGCAT+CGG - chr5.3:3615823-3615842 None:intergenic 55.0%
CGCAGCTAACACAAGAGGAG+TGG - chr5.3:3617654-3617673 None:intergenic 55.0%
GAGCATGCACCATGCTCAGT+TGG + chr5.3:3618181-3618200 MS.gene072756:CDS 55.0%
GATGCCAACAAGCCACCAAC+AGG - chr5.3:3618658-3618677 None:intergenic 55.0%
GCAAAGGCCAATTCACCGTC+CGG - chr5.3:3618604-3618623 None:intergenic 55.0%
GCGGAATTAGGACCTGTTGG+TGG + chr5.3:3618643-3618662 MS.gene072756:CDS 55.0%
TCTGGCTGCTGGGTTTGTCA+CGG + chr5.3:3617112-3617131 MS.gene072756:CDS 55.0%
TCTTGCAGTCACAAGGGGAC+TGG + chr5.3:3615909-3615928 MS.gene072756:CDS 55.0%
TGCGCGGAATTAGGACCTGT+TGG + chr5.3:3618640-3618659 MS.gene072756:CDS 55.0%
TTTGTTGATCGCGGGCTTGG+TGG + chr5.3:3618208-3618227 MS.gene072756:CDS 55.0%
! CATCAACCGGCTCACCTTCA+TGG - chr5.3:3617061-3617080 None:intergenic 55.0%
! CCGGCAAACGCTCCATCTTT+TGG - chr5.3:3617191-3617210 None:intergenic 55.0%
! TAGCTGCTGTTCTGGCTGCT+GGG + chr5.3:3617102-3617121 MS.gene072756:CDS 55.0%
! TTAGCTGCTGTTCTGGCTGC+TGG + chr5.3:3617101-3617120 MS.gene072756:CDS 55.0%
!! AGGACCTGTTGGTGGCTTGT+TGG + chr5.3:3618651-3618670 MS.gene072756:CDS 55.0%
AGCGTTTGCCGGTGCTCTTG+TGG + chr5.3:3617199-3617218 MS.gene072756:CDS 60.0%
CCATGACTGCAGCCCCTGAA+TGG - chr5.3:3617303-3617322 None:intergenic 60.0%
CCATTCAGGGGCTGCAGTCA+TGG + chr5.3:3617300-3617319 MS.gene072756:CDS 60.0%
CCGATGCAGCCTGCGTCTAA+TGG + chr5.3:3615820-3615839 MS.gene072756:CDS 60.0%
TCGAAGGCCGGACGGTGAAT+TGG + chr5.3:3618594-3618613 MS.gene072756:CDS 60.0%
TTAGACGCAGGCTGCATCGG+CGG - chr5.3:3615820-3615839 None:intergenic 60.0%
CTACTTGGGCCGTCAGCGCT+TGG + chr5.3:3616501-3616520 MS.gene072756:CDS 65.0%
CTTGTGGGTCGAAGGCCGGA+CGG + chr5.3:3618586-3618605 MS.gene072756:CDS 65.0%
GCTCGTATGGCCGAACACCC+TGG + chr5.3:3618322-3618341 MS.gene072756:CDS 65.0%
TTGGGCCGTCAGCGCTTGGA+CGG + chr5.3:3616505-3616524 MS.gene072756:CDS 65.0%
!! CAAGCGCTGACGGCCCAAGT+AGG - chr5.3:3616503-3616522 None:intergenic 65.0%
! ACGCAGGCTGCATCGGCGGA+GGG - chr5.3:3615816-3615835 None:intergenic 70.0%
! GACGCAGGCTGCATCGGCGG+AGG - chr5.3:3615817-3615836 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 3615781 3618798 3615781 ID=MS.gene072756
chr5.3 mRNA 3615781 3618798 3615781 ID=MS.gene072756.t1;Parent=MS.gene072756
chr5.3 exon 3615781 3615981 3615781 ID=MS.gene072756.t1.exon1;Parent=MS.gene072756.t1
chr5.3 CDS 3615781 3615981 3615781 ID=cds.MS.gene072756.t1;Parent=MS.gene072756.t1
chr5.3 exon 3616492 3617493 3616492 ID=MS.gene072756.t1.exon2;Parent=MS.gene072756.t1
chr5.3 CDS 3616492 3617493 3616492 ID=cds.MS.gene072756.t1;Parent=MS.gene072756.t1
chr5.3 exon 3617590 3618798 3617590 ID=MS.gene072756.t1.exon3;Parent=MS.gene072756.t1
chr5.3 CDS 3617590 3618798 3617590 ID=cds.MS.gene072756.t1;Parent=MS.gene072756.t1
Gene Sequence

>MS.gene072756

ATGACTTCCAATACCACAGCTAGAAATGTTTGCCCTCCGCCGATGCAGCCTGCGTCTAATGGTGTATTTCAAGGAGATCACCCTCTTGATTATGCACTTCCATTGGCTATTTTACAGATATGTCTTGTTCTTGCAGTCACAAGGGGACTGGCCTATCTTCTAAAACCTTTAAGGCAACCTCGAGTCATTGCAGAGATTGTTGTAAGTTCTTTTATTTTTGTTTAAGTATTTACTTAATTTAATGATTTTTTTTTGTTAACTGATATTAATAATTGATATTAGTAAAGAATTTTATATATTAGAAACTAGTGGGGAAATTGGCAAACAATGCAACAATAAGAGCCTTGAGTGTTCCATTTTTATATAATTTCACAGTTTTGAGTTTCAACTACGTTCTACTTGTCCAAACTATGAAACAAGTTCTAATATTTAGTCAATTTTTTATGTAGAGATTAGAATGCAATATGACTATAAAAAATTCACTGTTCTTAAATCAACAATAGATGTTAGAGAAATAGACATGCCAAAATGTGCACATGTTTAAGAGAAATTAAAATTGAATCACATTAAAATCGCAGAGAGTTTAAACACACTTGGAACTTCATTAAATCCACATTTGGTCAGTCTTACTAATTGCCTTCTAAAATAGAGCAGAGGTTTTTTCAACTTCTATTTTTGTGTGTTAATTAGATTGCTTATTGATGATGACAGGGAGGAATCCTACTTGGGCCGTCAGCGCTTGGACGGAATAAAAGCTATCTACATGCAGTTTTCCCACCCAAGAGTCTTCCAGTATTAGATACTCTAGCAAACCTTGGTCTTATATTCTTTCTATTTTTAGCAGGCATAGAGTTAGATCCTAAATCTCTGCGGAAAACTGGAGGTAGAGTCCTTGCCATTGCTATTGCCGGAATAAGCTTACCCTTTGCACTAGGAATTGGTTCATCGTTTGTTCTACAGGGAACAATTGCCAAAGGTGTAAATACTTCCGCATTTCTTGTATATATGGGGGTCGCTCTGTCTATAACTGCTTTTCCTGTATTAGCCCGTATTTTGGCCGAGTTAAAACTTCTAACCACTAGTGTCGGCAGAATGGCTATGTCAGCTGCAGCAGTTAATGATGTAGCAGCCTGGATTCTTCTTGCTCTTGCTGTTGCCTTATCAGGCAATAGCCAATCTCCATTTGTGTCATTGTGGGTCTTCTTAAGTGGGTGTGGTTTTGTTGTATGGTCAATCCTCATTGTCCTTCCAATTTTCAAATGGATGGCCCAACAATGCCATGAAGGTGAGCCGGTTGATGAGTTATACATATGTGCTACATTAGCTGCTGTTCTGGCTGCTGGGTTTGTCACGGATGCTATCGGAATTCATGCCATGTTTGGCCCGTTTGTTTTTGGAATTTTGGTTCCAAAAGATGGAGCGTTTGCCGGTGCTCTTGTGGAGAAAATAGAGGATCTTGTATCTGGTCTATTACTCCCACTTTATTTTGTGTCAAGTGGATTGAAGACCAACATAGCTACCATTCAGGGGCTGCAGTCATGGGGTCTTCTAGTTTTCGTTACATTTACGGCTTGTTTTGGGAAGATTGTTGGGACTATTGTAGTGTCTCTTCTATGTAAAGTACCTTTGAACGAGTCTTTAGTGCTTGGATTTTTGATGAATAGTAAAGGCTTGGTTGAATTAATTGTCCTCAACATCGGTAAAGATAGAAAGGTATTCGAATTTATTTTCTTGCTCCTCATTATTCCTATAATTAATTTAAAATGGTGACCGATCATCGTGATGTATATTTGATTTACGTATCTGCAGGTTTTGAATGATCAGACCTTTGCTATTATGGTTTTCATGGCTCTCATTACCACCTTCATGACCACTCCTCTTGTGTTAGCTGCGTATAAGAGGAAAGAAAGAAAATCTAATTACAAATACAGAACAATTGAAAGGAAAAATGCAGACGGCCAACTGAGGATTCTTGCCTGTTTCCATGGTTCAAGGAATATTCCATCGATGATAAACTTGATTGAGGCTTCAAGAGGAATCAAGAAGCATGACACACTTTGTGTCTATGCAATGCACCTTAAAGAATTTTGTGAGAGGTCCTCATCGATACTAATGGCACAAAAGGTAAGACAAAATGGGTTGCCGTTTTGGGATAAAGGTCACCATGGAGATTCTGTCCATGTTATTGTGGCGTTCGAGGCTTACCAAAAACTAAGCCACGTGTGTGTTCGACCAATGATAGCAATTTCATCAATGGCGAATATTCATGAAGACATTTGTGCAACTGCTGATAGAAAGAGAGCCGCTGTCATCATTCTTCCATTTCATAAGCAACAAAGGTTAGACGGTTCACTAGACATTATAAGAAACGATTTTCGATTGGTTAACAAAAGGGTACTCGAGCATGCACCATGCTCAGTTGGAATTTTTGTTGATCGCGGGCTTGGTGGTTCATGTCATGTGTCTGCAAGTAATGTTTCCTATTGCATTGTTGTGCTTTTCTTTGGTGGTGGTGATGACCGTGAAGCTCTTGCTTATGGAGCTCGTATGGCCGAACACCCTGGCATAAGATTAGTGGTTTTTCGCTTCTTATTGGAACCTAATATTGTAGGACAGATCACCAAAGTTGATGTTGGAGACTCTTCTAGCAGTAATTCAGTATCAGATTCTGAATATGACGAGTTTCTAGCTGAATTTAAGCTAAAAACAGCCAGTGATGACTCAATTATCTATGAAGAAAAAATAGTAAAAGATGCTGCAGAAACAGTTGCTACCATCCGCGTGATTAATTTCTGCAACTTATTTCTTGTGGGTCGAAGGCCGGACGGTGAATTGGCCTTTGCTCTTGAAAGAAGCGAGTGCGCGGAATTAGGACCTGTTGGTGGCTTGTTGGCATCACAAGATTTCCGCACAACAGCATCTGTTTTGGTGATGCAACAGTATCACAATGGGGTGCCAATAAACTTTGTCCCAGAAATGGAGGAACATTCACCTGATGGAGACACGGAATCTTCTTAA
Protein sequence

>MS.gene072756.t1

MTSNTTARNVCPPPMQPASNGVFQGDHPLDYALPLAILQICLVLAVTRGLAYLLKPLRQPRVIAEIVGGILLGPSALGRNKSYLHAVFPPKSLPVLDTLANLGLIFFLFLAGIELDPKSLRKTGGRVLAIAIAGISLPFALGIGSSFVLQGTIAKGVNTSAFLVYMGVALSITAFPVLARILAELKLLTTSVGRMAMSAAAVNDVAAWILLALAVALSGNSQSPFVSLWVFLSGCGFVVWSILIVLPIFKWMAQQCHEGEPVDELYICATLAAVLAAGFVTDAIGIHAMFGPFVFGILVPKDGAFAGALVEKIEDLVSGLLLPLYFVSSGLKTNIATIQGLQSWGLLVFVTFTACFGKIVGTIVVSLLCKVPLNESLVLGFLMNSKGLVELIVLNIGKDRKVLNDQTFAIMVFMALITTFMTTPLVLAAYKRKERKSNYKYRTIERKNADGQLRILACFHGSRNIPSMINLIEASRGIKKHDTLCVYAMHLKEFCERSSSILMAQKVRQNGLPFWDKGHHGDSVHVIVAFEAYQKLSHVCVRPMIAISSMANIHEDICATADRKRAAVIILPFHKQQRLDGSLDIIRNDFRLVNKRVLEHAPCSVGIFVDRGLGGSCHVSASNVSYCIVVLFFGGGDDREALAYGARMAEHPGIRLVVFRFLLEPNIVGQITKVDVGDSSSSNSVSDSEYDEFLAEFKLKTASDDSIIYEEKIVKDAAETVATIRVINFCNLFLVGRRPDGELAFALERSECAELGPVGGLLASQDFRTTASVLVMQQYHNGVPINFVPEMEEHSPDGDTESS