AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26746

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26746.t1 MTR_4g123583 98.644 811 11 0 1 811 1 811 0.0 1644
MS.gene26746.t1 MTR_4g078120 78.765 810 171 1 1 809 1 810 0.0 1297
MS.gene26746.t1 MTR_5g009780 59.698 794 304 4 3 793 6 786 0.0 969
MS.gene26746.t1 MTR_5g009770 60.076 789 297 4 3 788 6 779 0.0 966
MS.gene26746.t1 MTR_5g009800 56.726 788 304 6 9 793 12 765 0.0 907
MS.gene26746.t1 MTR_5g009770 58.673 663 256 4 129 788 1 648 0.0 791
MS.gene26746.t1 MTR_8g093780 45.865 798 413 10 1 786 1 791 0.0 726
MS.gene26746.t1 MTR_3g096380 45.558 788 413 9 6 786 12 790 0.0 707
MS.gene26746.t1 MTR_7g099800 51.578 824 336 22 12 787 8 816 0.0 691
MS.gene26746.t1 MTR_2g011520 43.918 781 410 9 15 786 3 764 0.0 671
MS.gene26746.t1 MTR_7g099750 48.736 831 346 20 12 789 8 811 0.0 663
MS.gene26746.t1 MTR_7g099820 48.120 798 368 15 13 784 5 782 0.0 654
MS.gene26746.t1 MTR_7g099820 52.941 663 286 9 13 657 5 659 0.0 632
MS.gene26746.t1 MTR_3g031170 33.079 786 482 10 8 785 29 778 7.82e-139 430
MS.gene26746.t1 MTR_2g040100 33.375 797 479 17 20 786 36 810 2.26e-132 415
MS.gene26746.t1 MTR_3g037270 32.107 788 491 14 20 786 25 789 1.18e-129 407
MS.gene26746.t1 MTR_5g067690 31.925 805 505 14 3 786 10 792 1.07e-128 404
MS.gene26746.t1 MTR_1g043400 31.936 811 493 17 7 786 3 785 7.14e-128 402
MS.gene26746.t1 MTR_3g037260 31.338 785 503 13 20 786 21 787 6.22e-126 397
MS.gene26746.t1 MTR_0002s1180 31.671 802 510 13 5 786 3 786 1.38e-125 396
MS.gene26746.t1 MTR_5g074360 30.525 819 509 18 3 798 14 795 2.37e-124 393
MS.gene26746.t1 MTR_1g045870 31.728 810 481 18 7 786 3 770 9.52e-123 388
MS.gene26746.t1 MTR_3g071990 30.025 796 501 16 6 785 10 765 1.32e-122 388
MS.gene26746.t1 MTR_5g074380 30.740 784 496 15 16 785 76 826 3.69e-121 386
MS.gene26746.t1 MTR_5g074340 29.963 811 503 18 15 802 30 798 1.74e-119 380
MS.gene26746.t1 MTR_5g074320 30.458 742 455 16 65 785 10 711 4.79e-108 348
MS.gene26746.t1 MTR_3g071990 30.278 720 446 17 82 785 4 683 8.70e-108 347
MS.gene26746.t1 MTR_3g031160 28.340 801 516 17 6 788 22 782 1.85e-104 340
MS.gene26746.t1 MTR_3g071880 27.945 798 515 20 18 784 22 790 8.23e-103 336
MS.gene26746.t1 MTR_3g498715 28.554 802 503 19 5 781 32 788 3.82e-98 323
MS.gene26746.t1 MTR_6g015940 29.383 810 499 19 3 785 22 785 5.57e-96 317
MS.gene26746.t1 MTR_4g037755 28.278 778 496 19 49 798 38 781 2.93e-95 315
MS.gene26746.t1 MTR_3g071870 28.714 801 506 21 16 785 33 799 1.40e-94 314
MS.gene26746.t1 MTR_6g053320 29.570 744 451 20 80 792 71 772 1.73e-94 313
MS.gene26746.t1 MTR_8g085260 27.950 805 505 18 10 785 31 789 9.62e-91 303
MS.gene26746.t1 MTR_5g088450 26.790 810 524 20 5 785 22 791 1.59e-88 297
MS.gene26746.t1 MTR_5g088790 27.833 812 519 16 5 785 39 814 1.92e-88 297
MS.gene26746.t1 MTR_3g083850 26.814 813 515 21 10 791 27 790 1.98e-88 297
MS.gene26746.t1 MTR_5g088430 26.601 812 522 21 10 791 27 794 5.23e-88 296
MS.gene26746.t1 MTR_5g088470 27.261 807 521 24 5 785 22 788 7.73e-88 295
MS.gene26746.t1 MTR_3g031030 27.771 785 501 18 39 795 28 774 8.73e-87 292
MS.gene26746.t1 MTR_7g053000 27.217 812 524 17 5 785 39 814 1.55e-86 292
MS.gene26746.t1 MTR_7g052680 27.340 812 523 17 5 785 39 814 6.61e-86 291
MS.gene26746.t1 MTR_7g052970 27.217 812 524 17 5 785 39 814 9.35e-86 290
MS.gene26746.t1 MTR_3g110700 26.889 781 533 16 24 786 15 775 1.53e-83 283
MS.gene26746.t1 MTR_4g029520 26.036 772 533 15 35 786 25 778 3.55e-83 282
MS.gene26746.t1 MTR_5g088840 26.788 825 512 20 5 785 39 815 4.49e-83 283
MS.gene26746.t1 MTR_7g085870 25.799 814 520 19 13 785 24 794 2.64e-82 281
MS.gene26746.t1 MTR_3g465680 30.534 393 265 6 17 404 44 433 5.98e-52 196
MS.gene26746.t1 MTR_3g465680 27.295 403 236 11 398 785 555 915 3.13e-34 141
MS.gene26746.t1 MTR_7g052895 25.665 526 358 8 5 505 39 556 6.01e-52 194
MS.gene26746.t1 MTR_1g082340 82.474 97 16 1 452 547 82 178 7.95e-49 172
MS.gene26746.t1 MTR_5g061460 80.412 97 18 1 452 547 15 111 7.48e-47 164
MS.gene26746.t1 MTR_5g083560 29.394 330 191 9 466 785 1 298 2.91e-25 108
MS.gene26746.t1 MTR_1g023830 79.592 49 9 1 500 547 1 49 9.24e-21 87.8
MS.gene26746.t1 MTR_3g083860 25.243 206 117 7 2 202 19 192 1.12e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26746.t1 AT3G17630 70.486 803 219 2 1 801 1 787 0.0 1132
MS.gene26746.t1 AT3G17630 70.486 803 219 2 1 801 1 787 0.0 1132
MS.gene26746.t1 AT5G41610 62.453 799 279 7 1 789 1 788 0.0 990
MS.gene26746.t1 AT5G41610 62.453 799 279 7 1 789 1 788 0.0 990
MS.gene26746.t1 AT4G23700 60.750 800 295 9 1 786 1 795 0.0 957
MS.gene26746.t1 AT4G23700 60.750 800 295 9 1 786 1 795 0.0 957
MS.gene26746.t1 AT5G41610 61.970 731 258 6 68 789 1 720 0.0 892
MS.gene26746.t1 AT1G64170 52.905 809 329 14 4 786 10 792 0.0 797
MS.gene26746.t1 AT2G13620 47.297 814 397 10 5 806 12 805 0.0 732
MS.gene26746.t1 AT3G53720 51.345 818 347 17 12 787 8 816 0.0 693
MS.gene26746.t1 AT3G53720 50.933 750 317 17 80 787 3 743 0.0 609
MS.gene26746.t1 AT1G05580 35.324 787 469 12 19 789 40 802 7.50e-148 456
MS.gene26746.t1 AT2G31910 32.783 787 489 13 19 788 26 789 7.54e-134 418
MS.gene26746.t1 AT1G05580 35.714 714 419 12 92 789 2 691 1.03e-133 416
MS.gene26746.t1 AT2G31910 33.053 714 438 13 92 788 2 692 2.21e-119 377
MS.gene26746.t1 AT2G31910 33.053 714 438 13 92 788 2 692 2.21e-119 377
MS.gene26746.t1 AT1G06970 31.380 819 511 18 5 811 25 804 2.36e-118 377
MS.gene26746.t1 AT2G30240 31.021 793 499 17 6 785 28 785 6.00e-105 342
MS.gene26746.t1 AT5G37060 26.129 819 536 12 6 785 41 829 2.54e-90 303
MS.gene26746.t1 AT5G01690 28.373 793 511 16 8 785 17 767 6.40e-89 298
MS.gene26746.t1 AT5G58460 25.935 802 533 12 18 785 53 827 1.29e-87 296
MS.gene26746.t1 AT3G44900 27.399 792 512 19 27 785 51 812 8.96e-87 293
MS.gene26746.t1 AT5G22900 26.087 828 522 20 11 785 27 817 1.15e-84 287
MS.gene26746.t1 AT3G52080 27.708 794 517 19 32 795 28 794 4.36e-79 271
MS.gene26746.t1 AT2G28180 27.538 788 513 18 28 791 61 814 5.20e-76 263
MS.gene26746.t1 AT1G79400 26.343 782 542 14 24 791 17 778 1.43e-75 261
MS.gene26746.t1 AT1G16380 25.283 795 536 17 24 791 17 780 1.41e-72 253
MS.gene26746.t1 AT5G01680 25.527 807 538 18 2 785 9 775 2.23e-70 247
MS.gene26746.t1 AT5G22910 26.422 791 502 21 21 774 37 784 2.67e-70 247
MS.gene26746.t1 AT1G08150 26.889 781 503 22 27 779 62 802 7.11e-70 246
MS.gene26746.t1 AT3G44910 25.548 775 499 16 28 776 35 757 6.39e-65 232
MS.gene26746.t1 AT3G44930 25.415 783 510 17 28 780 35 773 1.89e-64 231
MS.gene26746.t1 AT1G08140 25.095 793 517 20 28 785 53 803 1.23e-63 229
MS.gene26746.t1 AT2G28170 25.157 795 509 21 27 785 54 798 2.00e-62 225
MS.gene26746.t1 AT3G44920 25.316 790 508 18 28 783 35 776 1.13e-53 199
MS.gene26746.t1 AT1G08135 25.000 796 502 25 27 785 57 794 3.05e-53 199
MS.gene26746.t1 AT5G51710 22.759 435 274 13 33 439 154 554 6.58e-13 72.8
MS.gene26746.t1 AT5G51710 23.188 414 268 12 33 430 154 533 9.78e-13 72.0

Find 178 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTGTTCTGTTGGAATTT+TGG 0.120950 8.3:-4977359 MS.gene26746:CDS
CATCGATACGCTGGTGTTTA+TGG 0.150896 8.3:+4977443 None:intergenic
ATTGGAATTCATGCATTATT+TGG 0.159696 8.3:-4978707 MS.gene26746:CDS
AGCAAGTGAAGTTTCCTTTA+AGG 0.186167 8.3:-4977302 MS.gene26746:CDS
ACAAAGTCTTCCTCGCTTTC+GGG 0.227411 8.3:+4977726 None:intergenic
TGTCCCTTTCCTTGTCTTCA+TGG 0.230128 8.3:-4979080 MS.gene26746:CDS
TATCACATTGACATTGGTTT+TGG 0.230633 8.3:-4978753 MS.gene26746:CDS
ATTTGCAATTTGTGTTCTTA+TGG 0.243891 8.3:-4977938 MS.gene26746:CDS
TCCTCTTGTTATGGAGGAAT+TGG 0.251015 8.3:-4976771 MS.gene26746:CDS
GAAGATTACAACAACCTTAA+AGG 0.252758 8.3:+4977288 None:intergenic
TACAAAGTCTTCCTCGCTTT+CGG 0.253530 8.3:+4977725 None:intergenic
TGGACCTATTGGAAGTTATA+TGG 0.275025 8.3:-4976864 MS.gene26746:CDS
TCAGCTGAACTTGGACCTAT+TGG 0.290347 8.3:-4976875 MS.gene26746:CDS
CATTCATGCTCTTTGCACTA+TGG 0.290611 8.3:+4977035 None:intergenic
GTAACAATGGAATGCCCTTT+TGG 0.290967 8.3:-4977636 MS.gene26746:CDS
CTATAAGTGGAGGGGTTTCT+TGG 0.297538 8.3:-4978547 MS.gene26746:CDS
CTCCACTTCTGCATCAATCT+TGG 0.302772 8.3:-4976828 MS.gene26746:CDS
AGTGCAAGAGCTTCTCTAAA+AGG 0.305255 8.3:+4978443 None:intergenic
ATCACATTCCCATTTGTTCT+TGG 0.308008 8.3:-4979154 MS.gene26746:CDS
GAGCAAGAGTAATTTGATAT+TGG 0.309795 8.3:-4976942 MS.gene26746:CDS
CCTGGAATGACATTGTCTTT+TGG 0.310244 8.3:-4977169 MS.gene26746:CDS
AGGGACAGGTTGTGCACCTT+TGG 0.336201 8.3:+4979096 None:intergenic
AGGACTGTTAAGCTTCTCTT+TGG 0.339704 8.3:+4979292 None:intergenic
GCCTTAGCAATTGCCCTTTG+TGG 0.342396 8.3:-4979178 MS.gene26746:CDS
GCTCTTGCCATATCATTATC+TGG 0.347069 8.3:-4978923 MS.gene26746:CDS
TGATATTCATCCTCTTGTTA+TGG 0.348410 8.3:-4976780 MS.gene26746:CDS
CACTCCACAATTGTTCATCT+TGG 0.352984 8.3:+4977062 None:intergenic
TATCTATGCAATGCACTTGA+TGG 0.358211 8.3:-4977704 MS.gene26746:CDS
AAGGTTGTTGTAATCTTCTT+CGG 0.366064 8.3:-4977283 MS.gene26746:CDS
GTCCCTTTCCTTGTCTTCAT+GGG 0.366810 8.3:-4979079 MS.gene26746:CDS
CAAATGTAAAGCTCTTTAAC+AGG 0.373112 8.3:+4978776 None:intergenic
TGATATTGAGACAGCATTGA+TGG 0.373365 8.3:-4976969 MS.gene26746:CDS
TGTTAATGATGTTGCAGCTT+GGG 0.380742 8.3:-4978954 MS.gene26746:CDS
GTTATCTTTACTGCTTGCTT+TGG 0.382323 8.3:-4978509 MS.gene26746:CDS
GATCATGAAGCACTTGCTTA+TGG 0.387350 8.3:-4977250 MS.gene26746:CDS
CACTAGTACAAATATCTTCA+TGG 0.402538 8.3:+4977503 None:intergenic
GTTGGTGTGTCTGGCACTTC+AGG 0.403487 8.3:+4976743 None:intergenic
TTCCTCGCTTTCGGGTTCCT+CGG 0.404658 8.3:+4977734 None:intergenic
TCCTCGCTTTCGGGTTCCTC+GGG 0.406728 8.3:+4977735 None:intergenic
GTTCCTATGCCAAGAACAAA+TGG 0.410938 8.3:+4979145 None:intergenic
AACTAACGTGTTGGTGTGTC+TGG 0.412667 8.3:+4976734 None:intergenic
CGTCGCCCCTGATTTCACTC+TGG 0.415870 8.3:-4978892 MS.gene26746:CDS
TTGTATACTGCCATCACTAT+TGG 0.416249 8.3:+4977889 None:intergenic
GCATAACCCAGAGTGAAATC+AGG 0.417320 8.3:+4978885 None:intergenic
TTGTTCTTCTTATTCCTAGT+AGG 0.428237 8.3:-4979244 MS.gene26746:CDS
ACAACATGGAGTAAAATTCC+TGG 0.428599 8.3:+4977208 None:intergenic
TATAAGTGGAGGGGTTTCTT+GGG 0.428762 8.3:-4978546 MS.gene26746:CDS
CCAGCAAAAGGACCATCTTT+AGG 0.431587 8.3:+4978659 None:intergenic
TCTTTAACAGGTTCACCCTC+AGG 0.442719 8.3:+4978788 None:intergenic
TTACTAACCAATGGTGCAAC+CGG 0.444387 8.3:+4976905 None:intergenic
CTGAATCTTGATCTTTGCGT+TGG 0.445666 8.3:+4977827 None:intergenic
TGAAGAATCAATTAAATATG+AGG 0.447963 8.3:-4977014 MS.gene26746:CDS
CCAAAAGACAATGTCATTCC+AGG 0.449037 8.3:+4977169 None:intergenic
CTGCTTGGGCCATCAGCAAT+AGG 0.449639 8.3:-4979346 MS.gene26746:CDS
CTGTTAATGATGTTGCAGCT+TGG 0.451241 8.3:-4978955 MS.gene26746:CDS
ACAAAATGATGAAGATCAAA+TGG 0.457736 8.3:-4977605 MS.gene26746:CDS
TCTCATGAACACCAAGGGAT+TGG 0.457954 8.3:-4978414 MS.gene26746:CDS
AATGCCACCGGTTGCACCAT+TGG 0.458140 8.3:-4976912 MS.gene26746:CDS
AACAGTGATTCAGCTGAACT+TGG 0.462781 8.3:-4976884 MS.gene26746:CDS
GGACTGTTAAGCTTCTCTTT+GGG 0.464658 8.3:+4979293 None:intergenic
GATGGAACCATGGAGTCATT+AGG 0.464824 8.3:-4977424 MS.gene26746:CDS
GTCGCCCCTGATTTCACTCT+GGG 0.466919 8.3:-4978891 MS.gene26746:CDS
TCGCTTCGACCTATTGCTGA+TGG 0.467719 8.3:+4979337 None:intergenic
AGTCATGCACCTTGTTCTGT+TGG 0.468441 8.3:-4977367 MS.gene26746:CDS
ATGCGTTCAATCCGTAGAAC+AGG 0.468555 8.3:-4979208 MS.gene26746:CDS
AAGACCGTTGCTTTCATACA+TGG 0.468874 8.3:-4978822 MS.gene26746:CDS
GTTATCGCTTTCCAAACCTA+TGG 0.474315 8.3:-4977583 MS.gene26746:CDS
GAAGCCATATAACTTCCAAT+AGG 0.475088 8.3:+4976860 None:intergenic
TTTAGAGAAGCTCTTGCACT+TGG 0.475505 8.3:-4978440 MS.gene26746:CDS
TAAGGTGCTCCTCTTCGAGC+TGG 0.478801 8.3:+4977865 None:intergenic
GGTGCAGGACATGTCACATT+AGG 0.480678 8.3:+4980084 None:intergenic
CACTATTGGTGTGGTGATGA+AGG 0.486319 8.3:+4977903 None:intergenic
GAACACAAATTGCAAATGCT+TGG 0.489528 8.3:+4977944 None:intergenic
AAGAGTAATTTGATATTGGT+TGG 0.496000 8.3:-4976938 MS.gene26746:CDS
ACAAATGTGGCTACTATAAG+TGG 0.497636 8.3:-4978560 MS.gene26746:CDS
AAGATTGAAGATCTTGTGTC+TGG 0.498090 8.3:-4978623 MS.gene26746:CDS
CAATGTTGGCTAGTGTGTCT+AGG 0.501202 8.3:+4979272 None:intergenic
TTGTGCACCTTTGGAGATTG+TGG 0.501442 8.3:+4979105 None:intergenic
TGGGAATGTGATTCCACAAA+GGG 0.501645 8.3:+4979165 None:intergenic
AGATTCAGAGCTCAGAGTCT+TGG 0.507048 8.3:-4977812 MS.gene26746:CDS
CCGTTAGACGTGGCCTTCAT+CGG 0.507520 8.3:+4980063 None:intergenic
CACCAAGATTGATGCAGAAG+TGG 0.507651 8.3:+4976826 None:intergenic
AGCTAGGATTCGAGCGAGGA+CGG 0.508099 8.3:+4979030 None:intergenic
CCGATGAAGGCCACGTCTAA+CGG 0.510024 8.3:-4980063 MS.gene26746:CDS
GACGTGGCCTTCATCGGTGC+AGG 0.510090 8.3:+4980069 None:intergenic
TCAATCAAAACACCAGCAAA+AGG 0.510996 8.3:+4978647 None:intergenic
TCCACAAAGGGCAATTGCTA+AGG 0.512792 8.3:+4979177 None:intergenic
GAAGAACAAAAGACCAATGT+TGG 0.513906 8.3:+4979258 None:intergenic
TGCAGGGAGGAATACTGCTT+GGG 0.514991 8.3:-4979360 MS.gene26746:intron
GTCGTCGCCAGATAATGATA+TGG 0.518435 8.3:+4978916 None:intergenic
CATTTGTATCACATTGACAT+TGG 0.519281 8.3:-4978759 MS.gene26746:CDS
TGTCGAGTTCAAGACCTACT+AGG 0.520539 8.3:+4979230 None:intergenic
ATGCAGGGAGGAATACTGCT+TGG 0.520657 8.3:-4979361 MS.gene26746:intron
GCTAGGATTCGAGCGAGGAC+GGG 0.524066 8.3:+4979031 None:intergenic
GGTATAATTATGCCTAAAGA+TGG 0.525746 8.3:-4978671 MS.gene26746:CDS
TTCCCATTTGTTCTTGGCAT+AGG 0.530710 8.3:-4979148 MS.gene26746:CDS
CTTTAACAGGTTCACCCTCA+GGG 0.537753 8.3:+4978789 None:intergenic
ATGAGAATGGCAGAACATCC+AGG 0.538151 8.3:-4977226 MS.gene26746:CDS
GAGAGCAGAAATTGCTGTCA+TGG 0.538200 8.3:+4977534 None:intergenic
GTAAAGTTCATTTCTCCACC+TGG 0.538553 8.3:-4977187 MS.gene26746:CDS
TTCCTATGCCAAGAACAAAT+GGG 0.538714 8.3:+4979146 None:intergenic
CTACAAGGTTGATTAGTGTA+GGG 0.540493 8.3:+4977764 None:intergenic
TCTACAAGGTTGATTAGTGT+AGG 0.546671 8.3:+4977763 None:intergenic
AAACTACTAACCACTGATGT+TGG 0.547854 8.3:-4979001 MS.gene26746:CDS
ACCAAAATTCCAACAGAACA+AGG 0.549877 8.3:+4977358 None:intergenic
AATTAAATATGAGGAAAGGA+TGG 0.549955 8.3:-4977005 MS.gene26746:CDS
ACACCCATGAAGACAAGGAA+AGG 0.553825 8.3:+4979076 None:intergenic
CACCCATGAAGACAAGGAAA+GGG 0.558235 8.3:+4979077 None:intergenic
CATAACCCAGAGTGAAATCA+GGG 0.561076 8.3:+4978886 None:intergenic
CATAAACACCAGCGTATCGA+TGG 0.563693 8.3:-4977442 MS.gene26746:CDS
ATACGCTGGTGTTTATGGAA+TGG 0.565041 8.3:+4977448 None:intergenic
CTCAGTATCAATGCTATGCA+AGG 0.565314 8.3:-4978468 MS.gene26746:CDS
ATGGCTAAGCGTTCCCCTGA+GGG 0.570360 8.3:-4978803 MS.gene26746:CDS
TGAATGTCCTAATGACTCCA+TGG 0.570671 8.3:+4977417 None:intergenic
AATGAGCTCAACCAATCCCT+TGG 0.575721 8.3:+4978403 None:intergenic
TGCATGAATTCCAATAGTAT+CGG 0.577236 8.3:+4978715 None:intergenic
AGAGCAGAAATTGCTGTCAT+GGG 0.578202 8.3:+4977535 None:intergenic
TCCAATTCCTCCATAACAAG+AGG 0.579300 8.3:+4976770 None:intergenic
ACTTGCTTATGGTATGAGAA+TGG 0.579368 8.3:-4977239 MS.gene26746:CDS
GTTGTTTGAGTGGTTTGCAA+AGG 0.579896 8.3:+4979947 None:intergenic
TCTTTGGGAAAACTGTGTGT+AGG 0.583416 8.3:+4979308 None:intergenic
AAAACCAAGATGAACAATTG+TGG 0.583535 8.3:-4977066 MS.gene26746:CDS
TATTCATCCTCTTGTTATGG+AGG 0.583822 8.3:-4976777 MS.gene26746:CDS
AATCAATTAAATATGAGGAA+AGG 0.583986 8.3:-4977009 MS.gene26746:CDS
GACATGTCCTGCACCGATGA+AGG 0.584379 8.3:-4980076 MS.gene26746:CDS
GCTGGTGTTTATGGAATGGA+AGG 0.586936 8.3:+4977452 None:intergenic
TCACTCTGGGTTATGCTCTG+TGG 0.589152 8.3:-4978878 MS.gene26746:CDS
GAGCAACACCCATGAAGACA+AGG 0.593494 8.3:+4979071 None:intergenic
GGATTTCTCATGAACACCAA+GGG 0.596547 8.3:-4978419 MS.gene26746:CDS
CTAGACACACTAGCCAACAT+TGG 0.598588 8.3:-4979271 MS.gene26746:CDS
ATGAAGACAAGGAAAGGGAC+AGG 0.600424 8.3:+4979082 None:intergenic
TAGTGGTTTGAAGACAAATG+TGG 0.600827 8.3:-4978573 MS.gene26746:CDS
ATGACTCGAGGTTGTTTGAG+TGG 0.603036 8.3:+4979937 None:intergenic
GAATCACTGTTACTAACCAA+TGG 0.603558 8.3:+4976896 None:intergenic
TCCCGAGGAACCCGAAAGCG+AGG 0.605796 8.3:-4977736 MS.gene26746:CDS
GATGAAATCAACTAACGTGT+TGG 0.605876 8.3:+4976725 None:intergenic
GTTCACAAAGCACGTAACAA+TGG 0.609416 8.3:-4977649 MS.gene26746:CDS
CCATGGTTCCATCGATACGC+TGG 0.609696 8.3:+4977434 None:intergenic
GAGCTCATTGTTCTCAACAT+CGG 0.610796 8.3:-4978389 MS.gene26746:CDS
TTAGTAGTTTGAGCTCAGCT+AGG 0.611873 8.3:+4979014 None:intergenic
GTTGTTGTAATCTTCTTCGG+AGG 0.614692 8.3:-4977280 MS.gene26746:CDS
TTGGTTGATCGCGGTCTCGG+AGG 0.615743 8.3:-4977340 MS.gene26746:CDS
ATTGGTTGGTAGAATGCCAC+CGG 0.617567 8.3:-4976924 MS.gene26746:CDS
ATGATTTCTGCAATGACTCG+AGG 0.619902 8.3:+4979925 None:intergenic
TAGGGATGTTGCGTGTGCTG+TGG 0.621846 8.3:+4977782 None:intergenic
TTCCTCGGGATGATTCTACA+AGG 0.623083 8.3:+4977749 None:intergenic
ATGTGGCTACTATAAGTGGA+GGG 0.627903 8.3:-4978556 MS.gene26746:CDS
CTTAGAGCCACAATCTCCAA+AGG 0.629193 8.3:-4979112 MS.gene26746:CDS
CATCACCACACCAATAGTGA+TGG 0.629333 8.3:-4977899 MS.gene26746:CDS
TGTGGCTACTATAAGTGGAG+GGG 0.631044 8.3:-4978555 MS.gene26746:CDS
GATGTTGAAAGTAGCCATGA+TGG 0.632806 8.3:-4977094 MS.gene26746:CDS
CCACTAGAAGCAAAATAGAG+TGG 0.635208 8.3:+4978590 None:intergenic
GTTAATTCTTCAGATATGTG+TGG 0.635681 8.3:-4979998 MS.gene26746:CDS
AATGTGGCTACTATAAGTGG+AGG 0.637657 8.3:-4978557 MS.gene26746:CDS
AACGGAAGTGCATAATCGAG+AGG 0.638478 8.3:+4980021 None:intergenic
TTGGAATGCACCGTTAGACG+TGG 0.639960 8.3:+4980053 None:intergenic
CCAGCGTATCGATGGAACCA+TGG 0.641905 8.3:-4977434 MS.gene26746:CDS
TGGATTTCTCATGAACACCA+AGG 0.642435 8.3:-4978420 MS.gene26746:CDS
TGCTATACGACCAACATCAG+TGG 0.645855 8.3:+4978991 None:intergenic
ATGGGAATGTGATTCCACAA+AGG 0.646197 8.3:+4979164 None:intergenic
CATTGTTCTCAACATCGGAA+AGG 0.647673 8.3:-4978384 MS.gene26746:CDS
GTTACGTGCTTTGTGAACCA+TGG 0.649081 8.3:+4977654 None:intergenic
CAACATCGGAAAGGATCGCA+AGG 0.652219 8.3:-4978375 MS.gene26746:intron
CATGGCTAAGCGTTCCCCTG+AGG 0.653527 8.3:-4978804 MS.gene26746:CDS
CTAACCAATGGTGCAACCGG+TGG 0.654366 8.3:+4976908 None:intergenic
TACTGCCATCACTATTGGTG+TGG 0.659702 8.3:+4977894 None:intergenic
TTTAACAGGTTCACCCTCAG+GGG 0.666338 8.3:+4978790 None:intergenic
TTAGCCATGTATGAAAGCAA+CGG 0.666589 8.3:+4978818 None:intergenic
GAGAAATGAACTTTACAACA+TGG 0.677164 8.3:+4977194 None:intergenic
CATATCTGAAGAATTAACAA+CGG 0.681401 8.3:+4980003 None:intergenic
AAAGACAATGTCATTCCAGG+TGG 0.682922 8.3:+4977172 None:intergenic
GTATACAAACCAGCTCGAAG+AGG 0.689387 8.3:-4977874 MS.gene26746:CDS
GATTGTGGCTCTAAGAACAA+CGG 0.700703 8.3:+4979120 None:intergenic
GAATGGAAGGAGTATTATCG+CGG 0.711496 8.3:+4977465 None:intergenic
AACCTTGTAGAATCATCCCG+AGG 0.713913 8.3:-4977751 MS.gene26746:CDS
AAGATCTTCAGCTATCACCA+TGG 0.719007 8.3:-4977671 MS.gene26746:CDS
GCTCAGCTAGGATTCGAGCG+AGG 0.755526 8.3:+4979026 None:intergenic
ATAACCCAGAGTGAAATCAG+GGG 0.767323 8.3:+4978887 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATTAATCTTATAAAG+TGG - chr8.3:4977093-4977112 MS.gene26746:CDS 10.0%
!! AAAATATAACCATCAAATTA+AGG + chr8.3:4977001-4977020 None:intergenic 15.0%
!! AAATATAACCATCAAATTAA+GGG + chr8.3:4977000-4977019 None:intergenic 15.0%
!! TTGAAATATTTAACGATAAT+TGG + chr8.3:4978794-4978813 None:intergenic 15.0%
!!! ATTTTGTCATTAATTATTAG+AGG + chr8.3:4978738-4978757 None:intergenic 15.0%
!!! GTTTTATATTAAGATATAAG+TGG - chr8.3:4977344-4977363 MS.gene26746:CDS 15.0%
!! AATCAATTAAATATGAGGAA+AGG - chr8.3:4979820-4979839 MS.gene26746:intron 20.0%
!! TAATTATTAGAGGATCAATA+AGG + chr8.3:4978728-4978747 None:intergenic 20.0%
!! TCAGAAAAGAACTAAAATAT+CGG - chr8.3:4978610-4978629 MS.gene26746:CDS 20.0%
!! TGAAGAATCAATTAAATATG+AGG - chr8.3:4979815-4979834 MS.gene26746:intron 20.0%
!! TTTCAAATCTTTCATTACTT+TGG - chr8.3:4978808-4978827 MS.gene26746:CDS 20.0%
!! TTTGATAAAACAACAAATTG+TGG + chr8.3:4976971-4976990 None:intergenic 20.0%
!!! ATTTTGTTTTTTGTTCCAAA+AGG + chr8.3:4979211-4979230 None:intergenic 20.0%
!!! TGTTTTGTTTTGTAAATTAG+TGG - chr8.3:4977238-4977257 MS.gene26746:CDS 20.0%
!!! TTTTGTTTTTTGTTCCAAAA+GGG + chr8.3:4979210-4979229 None:intergenic 20.0%
! AAATTGATATGTAAGCTAGT+CGG + chr8.3:4977217-4977236 None:intergenic 25.0%
! AAGAGTAATTTGATATTGGT+TGG - chr8.3:4979891-4979910 MS.gene26746:intron 25.0%
! AATTAAATATGAGGAAAGGA+TGG - chr8.3:4979824-4979843 MS.gene26746:intron 25.0%
! ACAAAATGATGAAGATCAAA+TGG - chr8.3:4979224-4979243 MS.gene26746:CDS 25.0%
! ATCAAAATTGCACTGAAATA+TGG + chr8.3:4978587-4978606 None:intergenic 25.0%
! ATTGGAATTCATGCATTATT+TGG - chr8.3:4978122-4978141 MS.gene26746:intron 25.0%
! ATTTGCAATTTGTGTTCTTA+TGG - chr8.3:4978891-4978910 MS.gene26746:CDS 25.0%
! CAGAAAAGAACTAAAATATC+GGG - chr8.3:4978611-4978630 MS.gene26746:CDS 25.0%
! CATATCTGAAGAATTAACAA+CGG + chr8.3:4976829-4976848 None:intergenic 25.0%
! TGGACTAATGATACAAAATA+GGG + chr8.3:4976951-4976970 None:intergenic 25.0%
!! CGATGTTGGTTATTATATTA+AGG - chr8.3:4977288-4977307 MS.gene26746:CDS 25.0%
!!! ACCTTTTGAATTGATTTTGA+GGG - chr8.3:4977183-4977202 MS.gene26746:CDS 25.0%
!!! TACCTTTTGAATTGATTTTG+AGG - chr8.3:4977182-4977201 MS.gene26746:CDS 25.0%
!!! TTATTTGGTGCTTTTGTTAT+TGG - chr8.3:4978137-4978156 MS.gene26746:intron 25.0%
!!! TTTATTTTCTTCCATATGCA+CGG + chr8.3:4977144-4977163 None:intergenic 25.0%
AAAACCAAGATGAACAATTG+TGG - chr8.3:4979763-4979782 MS.gene26746:intron 30.0%
AAGGTTGTTGTAATCTTCTT+CGG - chr8.3:4979546-4979565 MS.gene26746:intron 30.0%
AGTTAGTGAGACAATGAATA+AGG - chr8.3:4977043-4977062 MS.gene26746:CDS 30.0%
ATGTGAAAATGTTCAATGCA+GGG - chr8.3:4977453-4977472 MS.gene26746:CDS 30.0%
CAAATGTAAAGCTCTTTAAC+AGG + chr8.3:4978056-4978075 None:intergenic 30.0%
CACTAGTACAAATATCTTCA+TGG + chr8.3:4979329-4979348 None:intergenic 30.0%
CATTTGTATCACATTGACAT+TGG - chr8.3:4978070-4978089 MS.gene26746:intron 30.0%
GAAGATTACAACAACCTTAA+AGG + chr8.3:4979544-4979563 None:intergenic 30.0%
GAGAAATGAACTTTACAACA+TGG + chr8.3:4979638-4979657 None:intergenic 30.0%
GAGCAAGAGTAATTTGATAT+TGG - chr8.3:4979887-4979906 MS.gene26746:intron 30.0%
GGACTAATGATACAAAATAG+GGG + chr8.3:4976950-4976969 None:intergenic 30.0%
GGATACTTCTAAGTATTACT+TGG + chr8.3:4978773-4978792 None:intergenic 30.0%
GGTATAATTATGCCTAAAGA+TGG - chr8.3:4978158-4978177 MS.gene26746:intron 30.0%
GTTAATTCTTCAGATATGTG+TGG - chr8.3:4976831-4976850 MS.gene26746:CDS 30.0%
TATGTGAAAATGTTCAATGC+AGG - chr8.3:4977452-4977471 MS.gene26746:CDS 30.0%
TCCCTCAAAATCAATTCAAA+AGG + chr8.3:4977187-4977206 None:intergenic 30.0%
TGATATTCATCCTCTTGTTA+TGG - chr8.3:4980049-4980068 MS.gene26746:CDS 30.0%
TGCATGAATTCCAATAGTAT+CGG + chr8.3:4978117-4978136 None:intergenic 30.0%
TTGTTCTTCTTATTCCTAGT+AGG - chr8.3:4977585-4977604 MS.gene26746:CDS 30.0%
! AAGAAAGTCCCTTAATTTGA+TGG - chr8.3:4976989-4977008 MS.gene26746:CDS 30.0%
! GTGGACTAATGATACAAAAT+AGG + chr8.3:4976952-4976971 None:intergenic 30.0%
! TGGACTGTTTTGTGTTTATA+AGG + chr8.3:4978985-4979004 None:intergenic 30.0%
! TGTTCAATTTTCCATAGGTT+TGG + chr8.3:4979260-4979279 None:intergenic 30.0%
! TTTGAAATCAAACGATGATG+TGG + chr8.3:4977120-4977139 None:intergenic 30.0%
!!! AGTTTTGTTACCGATACTAT+TGG - chr8.3:4978104-4978123 MS.gene26746:intron 30.0%
!!! ATTGTCTTTTGGTGCTAAAT+TGG - chr8.3:4979671-4979690 MS.gene26746:intron 30.0%
!!! TATCACATTGACATTGGTTT+TGG - chr8.3:4978076-4978095 MS.gene26746:intron 30.0%
AAACTACTAACCACTGATGT+TGG - chr8.3:4977828-4977847 MS.gene26746:CDS 35.0%
AAGATTGAAGATCTTGTGTC+TGG - chr8.3:4978206-4978225 MS.gene26746:intron 35.0%
ACAAATGTGGCTACTATAAG+TGG - chr8.3:4978269-4978288 MS.gene26746:intron 35.0%
ACAACATGGAGTAAAATTCC+TGG + chr8.3:4979624-4979643 None:intergenic 35.0%
ACCAAAATTCCAACAGAACA+AGG + chr8.3:4979474-4979493 None:intergenic 35.0%
ACTTGCTTATGGTATGAGAA+TGG - chr8.3:4979590-4979609 MS.gene26746:intron 35.0%
AGCAAGTGAAGTTTCCTTTA+AGG - chr8.3:4979527-4979546 MS.gene26746:intron 35.0%
ATCACATTCCCATTTGTTCT+TGG - chr8.3:4977675-4977694 MS.gene26746:CDS 35.0%
CATTTAGTACCTGTGTTACA+TGG + chr8.3:4978864-4978883 None:intergenic 35.0%
GAACACAAATTGCAAATGCT+TGG + chr8.3:4978888-4978907 None:intergenic 35.0%
GAAGAACAAAAGACCAATGT+TGG + chr8.3:4977574-4977593 None:intergenic 35.0%
GAAGCCATATAACTTCCAAT+AGG + chr8.3:4979972-4979991 None:intergenic 35.0%
GAATCACTGTTACTAACCAA+TGG + chr8.3:4979936-4979955 None:intergenic 35.0%
GTTATCTTTACTGCTTGCTT+TGG - chr8.3:4978320-4978339 MS.gene26746:intron 35.0%
TAGTGGTTTGAAGACAAATG+TGG - chr8.3:4978256-4978275 MS.gene26746:intron 35.0%
TATCTATGCAATGCACTTGA+TGG - chr8.3:4979125-4979144 MS.gene26746:CDS 35.0%
TCAATCAAAACACCAGCAAA+AGG + chr8.3:4978185-4978204 None:intergenic 35.0%
TCAGCAATCTCAAATATCCA+TGG - chr8.3:4978521-4978540 MS.gene26746:CDS 35.0%
TGATTTCAAATCCGTGCATA+TGG - chr8.3:4977130-4977149 MS.gene26746:CDS 35.0%
TGGACCTATTGGAAGTTATA+TGG - chr8.3:4979965-4979984 MS.gene26746:CDS 35.0%
TGTAATTCTCCATGTAACAC+AGG - chr8.3:4978852-4978871 MS.gene26746:CDS 35.0%
TGTCTCACTAACTTATTGCA+TGG + chr8.3:4977036-4977055 None:intergenic 35.0%
TGTTAATGATGTTGCAGCTT+GGG - chr8.3:4977875-4977894 MS.gene26746:CDS 35.0%
TTAGCCATGTATGAAAGCAA+CGG + chr8.3:4978014-4978033 None:intergenic 35.0%
TTCCTATGCCAAGAACAAAT+GGG + chr8.3:4977686-4977705 None:intergenic 35.0%
TTGTATACTGCCATCACTAT+TGG + chr8.3:4978943-4978962 None:intergenic 35.0%
! ACCTTGTTCTGTTGGAATTT+TGG - chr8.3:4979470-4979489 MS.gene26746:intron 35.0%
! CTACAAGGTTGATTAGTGTA+GGG + chr8.3:4979068-4979087 None:intergenic 35.0%
! GACACTGTTCAATTTTCCAT+AGG + chr8.3:4979265-4979284 None:intergenic 35.0%
! TATTCATCCTCTTGTTATGG+AGG - chr8.3:4980052-4980071 MS.gene26746:CDS 35.0%
! TCTACAAGGTTGATTAGTGT+AGG + chr8.3:4979069-4979088 None:intergenic 35.0%
! TGATATTGAGACAGCATTGA+TGG - chr8.3:4979860-4979879 MS.gene26746:intron 35.0%
!! CTTGGTTTTTACTACCATCA+TGG + chr8.3:4979752-4979771 None:intergenic 35.0%
!! GCTTGCTTTGGAAAAATTGT+TGG - chr8.3:4978332-4978351 MS.gene26746:intron 35.0%
!!! AGGCTTTTTTACCTGTTCTA+CGG + chr8.3:4977635-4977654 None:intergenic 35.0%
!!! TCTTTTGGTGCTAAATTGGT+TGG - chr8.3:4979675-4979694 MS.gene26746:intron 35.0%
AAAGACAATGTCATTCCAGG+TGG + chr8.3:4979660-4979679 None:intergenic 40.0%
AACAGTGATTCAGCTGAACT+TGG - chr8.3:4979945-4979964 MS.gene26746:CDS 40.0%
AAGATCTTCAGCTATCACCA+TGG - chr8.3:4979158-4979177 MS.gene26746:CDS 40.0%
AAGTGACAGCAACATTCAAC+AGG + chr8.3:4977420-4977439 None:intergenic 40.0%
AATGTGGCTACTATAAGTGG+AGG - chr8.3:4978272-4978291 MS.gene26746:intron 40.0%
AGAGCAGAAATTGCTGTCAT+GGG + chr8.3:4979297-4979316 None:intergenic 40.0%
AGGACTGTTAAGCTTCTCTT+TGG + chr8.3:4977540-4977559 None:intergenic 40.0%
AGTGCAAGAGCTTCTCTAAA+AGG + chr8.3:4978389-4978408 None:intergenic 40.0%
ATAACCCAGAGTGAAATCAG+GGG + chr8.3:4977945-4977964 None:intergenic 40.0%
ATACGCTGGTGTTTATGGAA+TGG + chr8.3:4979384-4979403 None:intergenic 40.0%
ATGATTTCTGCAATGACTCG+AGG + chr8.3:4976907-4976926 None:intergenic 40.0%
ATGGGAATGTGATTCCACAA+AGG + chr8.3:4977668-4977687 None:intergenic 40.0%
ATGTGGCTACTATAAGTGGA+GGG - chr8.3:4978273-4978292 MS.gene26746:intron 40.0%
CACTCCACAATTGTTCATCT+TGG + chr8.3:4979770-4979789 None:intergenic 40.0%
CATAACCCAGAGTGAAATCA+GGG + chr8.3:4977946-4977965 None:intergenic 40.0%
CATTCATGCTCTTTGCACTA+TGG + chr8.3:4979797-4979816 None:intergenic 40.0%
CATTGTTCTCAACATCGGAA+AGG - chr8.3:4978445-4978464 MS.gene26746:CDS 40.0%
CCAAAAGACAATGTCATTCC+AGG + chr8.3:4979663-4979682 None:intergenic 40.0%
CCACTAGAAGCAAAATAGAG+TGG + chr8.3:4978242-4978261 None:intergenic 40.0%
CGATCGAAATCTGGATAGAT+AGG + chr8.3:4978494-4978513 None:intergenic 40.0%
CTCAGTATCAATGCTATGCA+AGG - chr8.3:4978361-4978380 MS.gene26746:intron 40.0%
CTGAATCTTGATCTTTGCGT+TGG + chr8.3:4979005-4979024 None:intergenic 40.0%
CTGTTAATGATGTTGCAGCT+TGG - chr8.3:4977874-4977893 MS.gene26746:CDS 40.0%
GAATAATCGTGCATCTACAC+AGG - chr8.3:4977260-4977279 MS.gene26746:CDS 40.0%
GACTATAAGCGATCGAAATC+TGG + chr8.3:4978503-4978522 None:intergenic 40.0%
GAGCTCATTGTTCTCAACAT+CGG - chr8.3:4978440-4978459 MS.gene26746:CDS 40.0%
GATGTTGAAAGTAGCCATGA+TGG - chr8.3:4979735-4979754 MS.gene26746:intron 40.0%
GCATTTGGATCGAAATCAGA+CGG + chr8.3:4978650-4978669 None:intergenic 40.0%
GCTCTTGCCATATCATTATC+TGG - chr8.3:4977906-4977925 MS.gene26746:CDS 40.0%
GGACTGTTAAGCTTCTCTTT+GGG + chr8.3:4977539-4977558 None:intergenic 40.0%
GGATAGATAGGAAGACAGAT+AGG + chr8.3:4978482-4978501 None:intergenic 40.0%
GGATTTCTCATGAACACCAA+GGG - chr8.3:4978410-4978429 MS.gene26746:CDS 40.0%
GTAAAGTTCATTTCTCCACC+TGG - chr8.3:4979642-4979661 MS.gene26746:intron 40.0%
GTCAGTACATTAGTGGCATT+TGG + chr8.3:4978665-4978684 None:intergenic 40.0%
GTTATCGCTTTCCAAACCTA+TGG - chr8.3:4979246-4979265 MS.gene26746:CDS 40.0%
GTTCACAAAGCACGTAACAA+TGG - chr8.3:4979180-4979199 MS.gene26746:CDS 40.0%
GTTCCTATGCCAAGAACAAA+TGG + chr8.3:4977687-4977706 None:intergenic 40.0%
GTTGTTGTAATCTTCTTCGG+AGG - chr8.3:4979549-4979568 MS.gene26746:intron 40.0%
TAAGGCAGTCAGTACATTAG+TGG + chr8.3:4978672-4978691 None:intergenic 40.0%
TACAAAGTCTTCCTCGCTTT+CGG + chr8.3:4979107-4979126 None:intergenic 40.0%
TATAAGTGGAGGGGTTTCTT+GGG - chr8.3:4978283-4978302 MS.gene26746:intron 40.0%
TCCAATTCCTCCATAACAAG+AGG + chr8.3:4980062-4980081 None:intergenic 40.0%
TGAAAATGTTCAATGCAGGG+AGG - chr8.3:4977456-4977475 MS.gene26746:CDS 40.0%
TGAATGTCCTAATGACTCCA+TGG + chr8.3:4979415-4979434 None:intergenic 40.0%
TGGATTTCTCATGAACACCA+AGG - chr8.3:4978409-4978428 MS.gene26746:CDS 40.0%
TGGGAATGTGATTCCACAAA+GGG + chr8.3:4977667-4977686 None:intergenic 40.0%
TTACTAACCAATGGTGCAAC+CGG + chr8.3:4979927-4979946 None:intergenic 40.0%
TTAGTAGTTTGAGCTCAGCT+AGG + chr8.3:4977818-4977837 None:intergenic 40.0%
TTCCCATTTGTTCTTGGCAT+AGG - chr8.3:4977681-4977700 MS.gene26746:CDS 40.0%
TTTAGAGAAGCTCTTGCACT+TGG - chr8.3:4978389-4978408 MS.gene26746:CDS 40.0%
! AAGACCGTTGCTTTCATACA+TGG - chr8.3:4978007-4978026 MS.gene26746:intron 40.0%
! AATCGAGAGGATTTTCGTGT+TGG + chr8.3:4976798-4976817 None:intergenic 40.0%
! CCACTCTATTTTGCTTCTAG+TGG - chr8.3:4978239-4978258 MS.gene26746:intron 40.0%
! CCTGGAATGACATTGTCTTT+TGG - chr8.3:4979660-4979679 MS.gene26746:intron 40.0%
! GATCATGAAGCACTTGCTTA+TGG - chr8.3:4979579-4979598 MS.gene26746:intron 40.0%
! GTAACAATGGAATGCCCTTT+TGG - chr8.3:4979193-4979212 MS.gene26746:CDS 40.0%
! GTTGTTTGAGTGGTTTGCAA+AGG + chr8.3:4976885-4976904 None:intergenic 40.0%
! TCCTCTTGTTATGGAGGAAT+TGG - chr8.3:4980058-4980077 MS.gene26746:CDS 40.0%
! TCTTTGGGAAAACTGTGTGT+AGG + chr8.3:4977524-4977543 None:intergenic 40.0%
!! GAATGGAAGGAGTATTATCG+CGG + chr8.3:4979367-4979386 None:intergenic 40.0%
!! GATTGTGGCTCTAAGAACAA+CGG + chr8.3:4977712-4977731 None:intergenic 40.0%
!!! GTTGGAATTTTGGTTGATCG+CGG - chr8.3:4979480-4979499 MS.gene26746:intron 40.0%
AACCTTGTAGAATCATCCCG+AGG - chr8.3:4979078-4979097 MS.gene26746:CDS 45.0%
AATGAGCTCAACCAATCCCT+TGG + chr8.3:4978429-4978448 None:intergenic 45.0%
ACAAAGTCTTCCTCGCTTTC+GGG + chr8.3:4979106-4979125 None:intergenic 45.0%
ACACCCATGAAGACAAGGAA+AGG + chr8.3:4977756-4977775 None:intergenic 45.0%
AGATTCAGAGCTCAGAGTCT+TGG - chr8.3:4979017-4979036 MS.gene26746:CDS 45.0%
AGCTAGCAATCCACGCATTA+AGG + chr8.3:4978690-4978709 None:intergenic 45.0%
AGTCATGCACCTTGTTCTGT+TGG - chr8.3:4979462-4979481 MS.gene26746:intron 45.0%
ATGAAGACAAGGAAAGGGAC+AGG + chr8.3:4977750-4977769 None:intergenic 45.0%
ATGACTCGAGGTTGTTTGAG+TGG + chr8.3:4976895-4976914 None:intergenic 45.0%
ATGAGAATGGCAGAACATCC+AGG - chr8.3:4979603-4979622 MS.gene26746:intron 45.0%
ATGCGTTCAATCCGTAGAAC+AGG - chr8.3:4977621-4977640 MS.gene26746:CDS 45.0%
CACCAAGATTGATGCAGAAG+TGG + chr8.3:4980006-4980025 None:intergenic 45.0%
CACCCATGAAGACAAGGAAA+GGG + chr8.3:4977755-4977774 None:intergenic 45.0%
CATAAACACCAGCGTATCGA+TGG - chr8.3:4979387-4979406 MS.gene26746:intron 45.0%
CATCACCACACCAATAGTGA+TGG - chr8.3:4978930-4978949 MS.gene26746:CDS 45.0%
CATCGATACGCTGGTGTTTA+TGG + chr8.3:4979389-4979408 None:intergenic 45.0%
CCAGCAAAAGGACCATCTTT+AGG + chr8.3:4978173-4978192 None:intergenic 45.0%
CTAGACACACTAGCCAACAT+TGG - chr8.3:4977558-4977577 MS.gene26746:CDS 45.0%
CTATAAGTGGAGGGGTTTCT+TGG - chr8.3:4978282-4978301 MS.gene26746:intron 45.0%
CTCCACTTCTGCATCAATCT+TGG - chr8.3:4980001-4980020 MS.gene26746:CDS 45.0%
CTTAGAGCCACAATCTCCAA+AGG - chr8.3:4977717-4977736 MS.gene26746:CDS 45.0%
CTTTAACAGGTTCACCCTCA+GGG + chr8.3:4978043-4978062 None:intergenic 45.0%
GAGAGCAGAAATTGCTGTCA+TGG + chr8.3:4979298-4979317 None:intergenic 45.0%
GATGGAACCATGGAGTCATT+AGG - chr8.3:4979405-4979424 MS.gene26746:intron 45.0%
GCATAACCCAGAGTGAAATC+AGG + chr8.3:4977947-4977966 None:intergenic 45.0%
GCTGGTGTTTATGGAATGGA+AGG + chr8.3:4979380-4979399 None:intergenic 45.0%
GTATACAAACCAGCTCGAAG+AGG - chr8.3:4978955-4978974 MS.gene26746:CDS 45.0%
GTCCCTTTCCTTGTCTTCAT+GGG - chr8.3:4977750-4977769 MS.gene26746:CDS 45.0%
GTCGTCGCCAGATAATGATA+TGG + chr8.3:4977916-4977935 None:intergenic 45.0%
GTTACGTGCTTTGTGAACCA+TGG + chr8.3:4979178-4979197 None:intergenic 45.0%
TACTGCCATCACTATTGGTG+TGG + chr8.3:4978938-4978957 None:intergenic 45.0%
TCAGCTGAACTTGGACCTAT+TGG - chr8.3:4979954-4979973 MS.gene26746:CDS 45.0%
TCCACAAAGGGCAATTGCTA+AGG + chr8.3:4977655-4977674 None:intergenic 45.0%
TCTCATGAACACCAAGGGAT+TGG - chr8.3:4978415-4978434 MS.gene26746:CDS 45.0%
TCTTTAACAGGTTCACCCTC+AGG + chr8.3:4978044-4978063 None:intergenic 45.0%
TGCTATACGACCAACATCAG+TGG + chr8.3:4977841-4977860 None:intergenic 45.0%
TGTCCCTTTCCTTGTCTTCA+TGG - chr8.3:4977749-4977768 MS.gene26746:CDS 45.0%
TGTCGAGTTCAAGACCTACT+AGG + chr8.3:4977602-4977621 None:intergenic 45.0%
TGTGGCTACTATAAGTGGAG+GGG - chr8.3:4978274-4978293 MS.gene26746:intron 45.0%
TTCCTCGGGATGATTCTACA+AGG + chr8.3:4979083-4979102 None:intergenic 45.0%
TTTAACAGGTTCACCCTCAG+GGG + chr8.3:4978042-4978061 None:intergenic 45.0%
! AACGGAAGTGCATAATCGAG+AGG + chr8.3:4976811-4976830 None:intergenic 45.0%
! CAATGTTGGCTAGTGTGTCT+AGG + chr8.3:4977560-4977579 None:intergenic 45.0%
! CACTATTGGTGTGGTGATGA+AGG + chr8.3:4978929-4978948 None:intergenic 45.0%
! CCTAAAGATGGTCCTTTTGC+TGG - chr8.3:4978170-4978189 MS.gene26746:intron 45.0%
! TTGTGCACCTTTGGAGATTG+TGG + chr8.3:4977727-4977746 None:intergenic 45.0%
!! ATTGGTTGGTAGAATGCCAC+CGG - chr8.3:4979905-4979924 MS.gene26746:intron 45.0%
!!! ATTTTGGTTGATCGCGGTCT+CGG - chr8.3:4979486-4979505 MS.gene26746:intron 45.0%
ATGCAGGGAGGAATACTGCT+TGG - chr8.3:4977468-4977487 MS.gene26746:CDS 50.0%
CAACATCGGAAAGGATCGCA+AGG - chr8.3:4978454-4978473 MS.gene26746:CDS 50.0%
CTACACAGGTCTTGCGATGT+TGG - chr8.3:4977274-4977293 MS.gene26746:CDS 50.0%
GAGCAACACCCATGAAGACA+AGG + chr8.3:4977761-4977780 None:intergenic 50.0%
GCCTTAGCAATTGCCCTTTG+TGG - chr8.3:4977651-4977670 MS.gene26746:CDS 50.0%
GGTGCAGGACATGTCACATT+AGG + chr8.3:4976748-4976767 None:intergenic 50.0%
TCACTCTGGGTTATGCTCTG+TGG - chr8.3:4977951-4977970 MS.gene26746:CDS 50.0%
TCGCTTCGACCTATTGCTGA+TGG + chr8.3:4977495-4977514 None:intergenic 50.0%
TGCAGGGAGGAATACTGCTT+GGG - chr8.3:4977469-4977488 MS.gene26746:CDS 50.0%
TTGGAATGCACCGTTAGACG+TGG + chr8.3:4976779-4976798 None:intergenic 50.0%
!! GTACTGACTGCCTTAATGCG+TGG - chr8.3:4978677-4978696 MS.gene26746:CDS 50.0%
!! TCACTGATGTGCTGACTCCA+TGG + chr8.3:4978541-4978560 None:intergenic 50.0%
AGCTAGGATTCGAGCGAGGA+CGG + chr8.3:4977802-4977821 None:intergenic 55.0%
AGGGACAGGTTGTGCACCTT+TGG + chr8.3:4977736-4977755 None:intergenic 55.0%
ATGGCTAAGCGTTCCCCTGA+GGG - chr8.3:4978026-4978045 MS.gene26746:intron 55.0%
CCATGGTTCCATCGATACGC+TGG + chr8.3:4979398-4979417 None:intergenic 55.0%
CCGATGAAGGCCACGTCTAA+CGG - chr8.3:4976766-4976785 MS.gene26746:CDS 55.0%
CCGTTAGACGTGGCCTTCAT+CGG + chr8.3:4976769-4976788 None:intergenic 55.0%
CTAACCAATGGTGCAACCGG+TGG + chr8.3:4979924-4979943 None:intergenic 55.0%
CTGCTTGGGCCATCAGCAAT+AGG - chr8.3:4977483-4977502 MS.gene26746:CDS 55.0%
GTCGCCCCTGATTTCACTCT+GGG - chr8.3:4977938-4977957 MS.gene26746:CDS 55.0%
GTTGGTGTGTCTGGCACTTC+AGG + chr8.3:4980089-4980108 None:intergenic 55.0%
TAGGGATGTTGCGTGTGCTG+TGG + chr8.3:4979050-4979069 None:intergenic 55.0%
! AATGCCACCGGTTGCACCAT+TGG - chr8.3:4979917-4979936 MS.gene26746:intron 55.0%
! CCAGCGTATCGATGGAACCA+TGG - chr8.3:4979395-4979414 MS.gene26746:intron 55.0%
! GACATGTCCTGCACCGATGA+AGG - chr8.3:4976753-4976772 MS.gene26746:CDS 55.0%
! TAAGGTGCTCCTCTTCGAGC+TGG + chr8.3:4978967-4978986 None:intergenic 55.0%
!! TTCCTCGCTTTCGGGTTCCT+CGG + chr8.3:4979098-4979117 None:intergenic 55.0%
CATGGCTAAGCGTTCCCCTG+AGG - chr8.3:4978025-4978044 MS.gene26746:intron 60.0%
CGTCGCCCCTGATTTCACTC+TGG - chr8.3:4977937-4977956 MS.gene26746:CDS 60.0%
GCTAGGATTCGAGCGAGGAC+GGG + chr8.3:4977801-4977820 None:intergenic 60.0%
GCTCAGCTAGGATTCGAGCG+AGG + chr8.3:4977806-4977825 None:intergenic 60.0%
TTGGTTGATCGCGGTCTCGG+AGG - chr8.3:4979489-4979508 MS.gene26746:intron 60.0%
!! TCCTCGCTTTCGGGTTCCTC+GGG + chr8.3:4979097-4979116 None:intergenic 60.0%
GACGTGGCCTTCATCGGTGC+AGG + chr8.3:4976763-4976782 None:intergenic 65.0%
TCCCGAGGAACCCGAAAGCG+AGG - chr8.3:4979093-4979112 MS.gene26746:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 4976736 4980115 4976736 ID=MS.gene26746
chr8.3 mRNA 4976736 4980115 4976736 ID=MS.gene26746.t1;Parent=MS.gene26746
chr8.3 exon 4979924 4980115 4979924 ID=MS.gene26746.t1.exon1;Parent=MS.gene26746.t1
chr8.3 CDS 4979924 4980115 4979924 ID=cds.MS.gene26746.t1;Parent=MS.gene26746.t1
chr8.3 exon 4978376 4979377 4978376 ID=MS.gene26746.t1.exon2;Parent=MS.gene26746.t1
chr8.3 CDS 4978376 4979377 4978376 ID=cds.MS.gene26746.t1;Parent=MS.gene26746.t1
chr8.3 exon 4976736 4977977 4976736 ID=MS.gene26746.t1.exon3;Parent=MS.gene26746.t1
chr8.3 CDS 4976736 4977977 4976736 ID=cds.MS.gene26746.t1;Parent=MS.gene26746.t1
Gene Sequence

>MS.gene26746

ATGGCGACGCCTAATGTGACATGTCCTGCACCGATGAAGGCCACGTCTAACGGTGCATTCCAACACGAAAATCCTCTCGATTATGCACTTCCGTTGTTAATTCTTCAGATATGTGTGGTTGTCACATTCACTAGATGTATTGCATACCTTTGCAAACCACTCAAACAACCTCGAGTCATTGCAGAAATCATTGTAAGTTGTCTTCTCTTTTCCCCTATTTTGTATCATTAGTCCACAATTTGTTGTTTTATCAAAGAAAGTCCCTTAATTTGATGGTTATATTTTATATTTGAGTTTCCATGCAATAAGTTAGTGAGACAATGAATAAGGCAGTGTGACATGATTGACAAATTATTATAATTATTAATCTTATAAAGTGGTCCACATCATCGTTTGATTTCAAATCCGTGCATATGGAAGAAAATAAAAATTGTCAATGAGAATTTTACCTTTTGAATTGATTTTGAGGGATTAGTCTCCGACTAGCTTACATATCAATTTTTGTTTTGTTTTGTAAATTAGTGGAATAATCGTGCATCTACACAGGTCTTGCGATGTTGGTTATTATATTAAGGTAGTTAATATAAGATTAAAATGTTTAGAATTTTGTTTTATATTAAGATATAAGTGGAATTATTGAAACGTAATTTCTTCTGTTGCAAGTAAAACTTTTGTATGTTGCCTGTTGAATGTTGCTGTCACTTTTGTAAGTTTCTTATGTGAAAATGTTCAATGCAGGGAGGAATACTGCTTGGGCCATCAGCAATAGGTCGAAGCGAGAAATTCCTACACACAGTTTTCCCAAAGAGAAGCTTAACAGTCCTAGACACACTAGCCAACATTGGTCTTTTGTTCTTCTTATTCCTAGTAGGTCTTGAACTCGACATGCGTTCAATCCGTAGAACAGGTAAAAAAGCCTTAGCAATTGCCCTTTGTGGAATCACATTCCCATTTGTTCTTGGCATAGGAACATCCGTTGTTCTTAGAGCCACAATCTCCAAAGGTGCACAACCTGTCCCTTTCCTTGTCTTCATGGGTGTTGCTCTCTCAATCACAGCATTTCCCGTCCTCGCTCGAATCCTAGCTGAGCTCAAACTACTAACCACTGATGTTGGTCGTATAGCAATGTCAGCAGCTGCTGTTAATGATGTTGCAGCTTGGGTACTTCTTGCTCTTGCCATATCATTATCTGGCGACGACACGTCGCCCCTGATTTCACTCTGGGTTATGCTCTGTGGTGCTGCTTTTATTCTCTTTGCTGTTTTTGCTATAAGACCGTTGCTTTCATACATGGCTAAGCGTTCCCCTGAGGGTGAACCTGTTAAAGAGCTTTACATTTGTATCACATTGACATTGGTTTTGGCTTGTAGTTTTGTTACCGATACTATTGGAATTCATGCATTATTTGGTGCTTTTGTTATTGGTATAATTATGCCTAAAGATGGTCCTTTTGCTGGTGTTTTGATTGAGAAGATTGAAGATCTTGTGTCTGGAATCTTTTTGCCACTCTATTTTGCTTCTAGTGGTTTGAAGACAAATGTGGCTACTATAAGTGGAGGGGTTTCTTGGGCACTATTATTACTAGTTATCTTTACTGCTTGCTTTGGAAAAATTGTTGGTACAATCTCAGTATCAATGCTATGCAAGGTGCCTTTTAGAGAAGCTCTTGCACTTGGATTTCTCATGAACACCAAGGGATTGGTTGAGCTCATTGTTCTCAACATCGGAAAGGATCGCAAGGTACCTATCTGTCTTCCTATCTATCCAGATTTCGATCGCTTATAGTCAGCAATCTCAAATATCCATGGAGTCAGCACATCAGTGACTTTTTGATCGAGATCAGACTATCCATATTTCAGTGCAATTTTGATTTTTCAGAAAAGAACTAAAATATCGGGTCTGAAGTTTGAACCGTCTGATTTCGATCCAAATGCCACTAATGTACTGACTGCCTTAATGCGTGGATTGCTAGCTGCAGACAATCTAAATCCTTATTGATCCTCTAATAATTAATGACAAAATAAAACTTGTTTTCCAAGTAATACTTAGAAGTATCCAATTATCGTTAAATATTTCAAATCTTTCATTACTTTGGTTTGATTTTTTATGCATGTAATGTAATTCTCCATGTAACACAGGTACTAAATGACCAAGCATTTGCAATTTGTGTTCTTATGGCATTATTCACAACCTTCATCACCACACCAATAGTGATGGCAGTATACAAACCAGCTCGAAGAGGAGCACCTTATAAACACAAAACAGTCCAACGCAAAGATCAAGATTCAGAGCTCAGAGTCTTGGCATGTTTCCACAGCACACGCAACATCCCTACACTAATCAACCTTGTAGAATCATCCCGAGGAACCCGAAAGCGAGGAAGACTTTGTATCTATGCAATGCACTTGATGGAACTCTCAGAAAGATCTTCAGCTATCACCATGGTTCACAAAGCACGTAACAATGGAATGCCCTTTTGGAACAAAAAACAAAATGATGAAGATCAAATGGTTATCGCTTTCCAAACCTATGGAAAATTGAACAGTGTCAATGTTCGTCCCATGACAGCAATTTCTGCTCTCAGCAACATCCATGAAGATATTTGTACTAGTGCTCATCAAAAGCGCGCCGCGATAATACTCCTTCCATTCCATAAACACCAGCGTATCGATGGAACCATGGAGTCATTAGGACATTCATTTCATGTAATCAATGAGCTTGTGTTGAGTCATGCACCTTGTTCTGTTGGAATTTTGGTTGATCGCGGTCTCGGAGGAACAAGTCAAGTACAAGCAAGTGAAGTTTCCTTTAAGGTTGTTGTAATCTTCTTCGGAGGACGCGACGATCATGAAGCACTTGCTTATGGTATGAGAATGGCAGAACATCCAGGAATTTTACTCCATGTTGTAAAGTTCATTTCTCCACCTGGAATGACATTGTCTTTTGGTGCTAAATTGGTTGGTGTAGCACAAGATAAGAGCAAAACAGTTGTGATTGATGATGTTGAAAGTAGCCATGATGGTAGTAAAAACCAAGATGAACAATTGTGGAGTGAATTCCATAGTGCAAAGAGCATGAATGAAGAATCAATTAAATATGAGGAAAGGATGGTTGAAAGTAAAAATGATATTGAGACAGCATTGATGGAATTGAGCAAGAGTAATTTGATATTGGTTGGTAGAATGCCACCGGTTGCACCATTGGTTAGTAACAGTGATTCAGCTGAACTTGGACCTATTGGAAGTTATATGGCTTCTTCTAGTTTCTCCACTTCTGCATCAATCTTGGTGATTCAACAATATAATTCAACAACTGATATTCATCCTCTTGTTATGGAGGAATTGGATTGTCCTGAAGTGCCAGACACACCAACACGTTAG
Protein sequence

>MS.gene26746.t1

MATPNVTCPAPMKATSNGAFQHENPLDYALPLLILQICVVVTFTRCIAYLCKPLKQPRVIAEIIGGILLGPSAIGRSEKFLHTVFPKRSLTVLDTLANIGLLFFLFLVGLELDMRSIRRTGKKALAIALCGITFPFVLGIGTSVVLRATISKGAQPVPFLVFMGVALSITAFPVLARILAELKLLTTDVGRIAMSAAAVNDVAAWVLLALAISLSGDDTSPLISLWVMLCGAAFILFAVFAIRPLLSYMAKRSPEGEPVKELYICITLTLVLACSFVTDTIGIHALFGAFVIGIIMPKDGPFAGVLIEKIEDLVSGIFLPLYFASSGLKTNVATISGGVSWALLLLVIFTACFGKIVGTISVSMLCKVPFREALALGFLMNTKGLVELIVLNIGKDRKVLNDQAFAICVLMALFTTFITTPIVMAVYKPARRGAPYKHKTVQRKDQDSELRVLACFHSTRNIPTLINLVESSRGTRKRGRLCIYAMHLMELSERSSAITMVHKARNNGMPFWNKKQNDEDQMVIAFQTYGKLNSVNVRPMTAISALSNIHEDICTSAHQKRAAIILLPFHKHQRIDGTMESLGHSFHVINELVLSHAPCSVGILVDRGLGGTSQVQASEVSFKVVVIFFGGRDDHEALAYGMRMAEHPGILLHVVKFISPPGMTLSFGAKLVGVAQDKSKTVVIDDVESSHDGSKNQDEQLWSEFHSAKSMNEESIKYEERMVESKNDIETALMELSKSNLILVGRMPPVAPLVSNSDSAELGPIGSYMASSSFSTSASILVIQQYNSTTDIHPLVMEELDCPEVPDTPTR