AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030201

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030201.t1 MTR_4g123583 98.889 810 9 0 1 810 1 810 0.0 1644
MS.gene030201.t1 MTR_4g078120 78.765 810 171 1 1 809 1 810 0.0 1297
MS.gene030201.t1 MTR_5g009780 59.572 794 305 4 3 793 6 786 0.0 968
MS.gene030201.t1 MTR_5g009770 59.949 789 298 4 3 788 6 779 0.0 964
MS.gene030201.t1 MTR_5g009800 56.599 788 305 6 9 793 12 765 0.0 906
MS.gene030201.t1 MTR_5g009770 58.522 663 257 4 129 788 1 648 0.0 789
MS.gene030201.t1 MTR_8g093780 45.739 798 414 10 1 786 1 791 0.0 723
MS.gene030201.t1 MTR_3g096380 45.443 790 411 10 6 786 12 790 0.0 706
MS.gene030201.t1 MTR_7g099800 51.816 826 331 23 12 787 8 816 0.0 691
MS.gene030201.t1 MTR_2g011520 43.918 781 410 9 15 786 3 764 0.0 671
MS.gene030201.t1 MTR_7g099750 48.678 832 345 20 12 789 8 811 0.0 660
MS.gene030201.t1 MTR_7g099820 48.120 798 368 15 13 784 5 782 0.0 652
MS.gene030201.t1 MTR_7g099820 52.941 663 286 9 13 657 5 659 0.0 632
MS.gene030201.t1 MTR_3g031170 33.206 786 481 10 8 785 29 778 1.01e-139 432
MS.gene030201.t1 MTR_2g040100 33.375 797 479 17 20 786 36 810 3.74e-132 414
MS.gene030201.t1 MTR_3g037270 32.107 788 491 14 20 786 25 789 9.61e-130 407
MS.gene030201.t1 MTR_5g067690 32.050 805 504 14 3 786 10 792 8.36e-129 404
MS.gene030201.t1 MTR_1g043400 31.773 812 493 16 7 786 3 785 8.31e-128 402
MS.gene030201.t1 MTR_3g037260 31.345 788 499 14 20 786 21 787 6.12e-126 397
MS.gene030201.t1 MTR_0002s1180 31.546 802 511 13 5 786 3 786 2.62e-125 395
MS.gene030201.t1 MTR_5g074360 30.976 820 504 19 3 798 14 795 1.91e-124 393
MS.gene030201.t1 MTR_1g045870 31.566 811 481 17 7 786 3 770 9.37e-123 388
MS.gene030201.t1 MTR_3g071990 30.025 796 501 16 6 785 10 765 1.83e-122 388
MS.gene030201.t1 MTR_5g074380 31.083 785 492 16 16 785 76 826 3.34e-121 386
MS.gene030201.t1 MTR_5g074340 29.963 811 503 18 15 802 30 798 1.29e-119 380
MS.gene030201.t1 MTR_5g074320 30.821 743 451 17 65 785 10 711 9.80e-109 350
MS.gene030201.t1 MTR_3g071990 30.278 720 446 17 82 785 4 683 1.10e-107 347
MS.gene030201.t1 MTR_3g031160 28.340 801 516 17 6 788 22 782 1.30e-104 340
MS.gene030201.t1 MTR_3g071880 27.945 798 515 20 18 784 22 790 2.39e-102 335
MS.gene030201.t1 MTR_3g498715 28.554 802 503 19 5 781 32 788 9.49e-98 322
MS.gene030201.t1 MTR_6g015940 29.383 810 499 19 3 785 22 785 6.11e-96 317
MS.gene030201.t1 MTR_4g037755 28.278 778 496 19 49 798 38 781 2.70e-95 315
MS.gene030201.t1 MTR_6g053320 30.108 744 447 21 80 792 71 772 5.16e-95 314
MS.gene030201.t1 MTR_3g071870 28.714 801 506 21 16 785 33 799 1.13e-94 314
MS.gene030201.t1 MTR_8g085260 27.736 804 508 17 10 785 31 789 2.30e-90 302
MS.gene030201.t1 MTR_5g088790 27.662 817 514 16 5 785 39 814 5.02e-89 299
MS.gene030201.t1 MTR_5g088450 26.790 810 524 20 5 785 22 791 1.15e-88 298
MS.gene030201.t1 MTR_3g083850 26.814 813 515 21 10 791 27 790 1.37e-88 297
MS.gene030201.t1 MTR_5g088430 26.601 812 522 21 10 791 27 794 5.02e-88 296
MS.gene030201.t1 MTR_5g088470 27.261 807 521 24 5 785 22 788 7.27e-88 295
MS.gene030201.t1 MTR_7g053000 27.050 817 519 17 5 785 39 814 3.26e-87 294
MS.gene030201.t1 MTR_3g031030 27.898 785 500 18 39 795 28 774 9.28e-87 292
MS.gene030201.t1 MTR_7g052970 27.050 817 519 17 5 785 39 814 1.60e-86 292
MS.gene030201.t1 MTR_7g052680 27.173 817 518 17 5 785 39 814 1.67e-86 292
MS.gene030201.t1 MTR_4g029520 26.166 772 532 15 35 786 25 778 3.51e-84 285
MS.gene030201.t1 MTR_3g110700 26.982 782 531 17 24 786 15 775 5.52e-83 282
MS.gene030201.t1 MTR_5g088840 27.050 817 520 19 5 785 39 815 1.43e-82 281
MS.gene030201.t1 MTR_7g085870 26.167 814 517 20 13 785 24 794 2.36e-82 281
MS.gene030201.t1 MTR_7g052895 25.665 526 358 8 5 505 39 556 4.78e-52 194
MS.gene030201.t1 MTR_3g465680 30.534 393 265 6 17 404 44 433 7.12e-52 196
MS.gene030201.t1 MTR_3g465680 27.543 403 235 11 398 785 555 915 5.39e-35 144
MS.gene030201.t1 MTR_1g082340 82.474 97 16 1 452 547 82 178 7.90e-49 172
MS.gene030201.t1 MTR_5g061460 80.412 97 18 1 452 547 15 111 7.97e-47 164
MS.gene030201.t1 MTR_5g083560 29.697 330 190 9 466 785 1 298 2.26e-25 108
MS.gene030201.t1 MTR_1g023830 79.592 49 9 1 500 547 1 49 9.23e-21 87.8
MS.gene030201.t1 MTR_3g083860 25.243 206 117 7 2 202 19 192 1.20e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030201.t1 AT3G17630 70.647 804 216 3 1 801 1 787 0.0 1132
MS.gene030201.t1 AT3G17630 70.647 804 216 3 1 801 1 787 0.0 1132
MS.gene030201.t1 AT5G41610 62.453 799 279 7 1 789 1 788 0.0 988
MS.gene030201.t1 AT5G41610 62.453 799 279 7 1 789 1 788 0.0 988
MS.gene030201.t1 AT4G23700 60.875 800 294 9 1 786 1 795 0.0 958
MS.gene030201.t1 AT4G23700 60.875 800 294 9 1 786 1 795 0.0 958
MS.gene030201.t1 AT5G41610 61.970 731 258 6 68 789 1 720 0.0 891
MS.gene030201.t1 AT1G64170 52.905 809 329 14 4 786 10 792 0.0 798
MS.gene030201.t1 AT2G13620 47.297 814 397 10 5 806 12 805 0.0 731
MS.gene030201.t1 AT3G53720 51.345 818 347 17 12 787 8 816 0.0 692
MS.gene030201.t1 AT3G53720 50.933 750 317 17 80 787 3 743 0.0 608
MS.gene030201.t1 AT1G05580 35.451 787 468 12 19 789 40 802 1.41e-147 455
MS.gene030201.t1 AT1G05580 35.854 714 418 12 92 789 2 691 1.36e-133 415
MS.gene030201.t1 AT2G31910 33.037 787 487 14 19 788 26 789 1.57e-133 417
MS.gene030201.t1 AT2G31910 33.333 714 436 14 92 788 2 692 2.86e-119 377
MS.gene030201.t1 AT2G31910 33.333 714 436 14 92 788 2 692 2.86e-119 377
MS.gene030201.t1 AT1G06970 31.778 793 496 16 5 785 25 784 3.19e-118 377
MS.gene030201.t1 AT2G30240 31.021 793 499 17 6 785 28 785 8.88e-105 342
MS.gene030201.t1 AT5G37060 26.773 818 532 13 6 785 41 829 5.65e-91 305
MS.gene030201.t1 AT5G01690 28.247 793 512 16 8 785 17 767 7.77e-89 297
MS.gene030201.t1 AT5G58460 26.368 804 527 13 18 785 53 827 1.25e-88 299
MS.gene030201.t1 AT3G44900 27.399 792 512 19 27 785 51 812 9.43e-87 293
MS.gene030201.t1 AT5G22900 26.087 828 522 20 11 785 27 817 1.64e-84 287
MS.gene030201.t1 AT3G52080 27.708 794 517 19 32 795 28 794 8.76e-79 271
MS.gene030201.t1 AT1G79400 26.343 782 542 14 24 791 17 778 1.85e-76 264
MS.gene030201.t1 AT2G28180 27.538 788 513 18 28 791 61 814 1.04e-75 263
MS.gene030201.t1 AT1G16380 25.409 795 535 17 24 791 17 780 1.16e-72 253
MS.gene030201.t1 AT5G22910 26.422 791 502 21 21 774 37 784 3.02e-70 247
MS.gene030201.t1 AT5G01680 25.403 807 539 18 2 785 9 775 3.36e-70 247
MS.gene030201.t1 AT1G08150 26.761 781 504 22 27 779 62 802 2.12e-69 245
MS.gene030201.t1 AT3G44930 25.546 779 506 17 28 776 35 769 1.31e-65 234
MS.gene030201.t1 AT3G44910 25.548 775 499 16 28 776 35 757 1.45e-64 231
MS.gene030201.t1 AT1G08140 25.095 793 517 20 28 785 53 803 1.19e-63 229
MS.gene030201.t1 AT2G28170 25.377 796 506 23 27 785 54 798 1.53e-62 225
MS.gene030201.t1 AT3G44920 25.443 790 507 18 28 783 35 776 2.35e-54 202
MS.gene030201.t1 AT1G08135 24.938 802 495 26 27 785 57 794 2.11e-53 199
MS.gene030201.t1 AT5G51710 22.759 435 274 13 33 439 154 554 6.74e-13 72.8
MS.gene030201.t1 AT5G51710 23.188 414 268 12 33 430 154 533 1.07e-12 72.0

Find 180 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTGTTCTGTTGGAATTT+TGG 0.120950 8.2:-9490161 MS.gene030201:CDS
CATCGATACGCTGGTGTTTA+TGG 0.150896 8.2:+9490245 None:intergenic
ATTGGAATTCATGCATTATT+TGG 0.159696 8.2:-9491512 MS.gene030201:CDS
AGCAAGTGAAGTTTCCTTTA+AGG 0.186167 8.2:-9490104 MS.gene030201:CDS
ACAAAGTCTTCCTCGCTTTC+GGG 0.227411 8.2:+9490528 None:intergenic
TGTCCCTTTCCTTGTCTTCA+TGG 0.230128 8.2:-9491885 MS.gene030201:CDS
TATCACATTGACATTGGTTT+TGG 0.230633 8.2:-9491558 MS.gene030201:CDS
ATTTGCAATTTGTGTTCTTA+TGG 0.243891 8.2:-9490740 MS.gene030201:CDS
TCCTCTTGTTATGGAGGAAT+TGG 0.251015 8.2:-9489573 MS.gene030201:CDS
GAAGATTACAACAACCTTAA+AGG 0.252758 8.2:+9490090 None:intergenic
TACAAAGTCTTCCTCGCTTT+CGG 0.253530 8.2:+9490527 None:intergenic
TGTATACTGCCATCACTATT+GGG 0.269576 8.2:+9490692 None:intergenic
GCAACAATGGAATGCCCTTT+TGG 0.272307 8.2:-9490438 MS.gene030201:CDS
TCAGCTGAACTTGGACCTAT+TGG 0.290347 8.2:-9489677 MS.gene030201:CDS
CATTCATGCTCTTTGCACTA+TGG 0.290611 8.2:+9489837 None:intergenic
CTATAAGTGGAGGGGTTTCT+TGG 0.297538 8.2:-9491352 MS.gene030201:CDS
CTCCACTTCTGCATCAATCT+TGG 0.302772 8.2:-9489630 MS.gene030201:CDS
AGTGCAAGAGCTTCTCTAAA+AGG 0.305255 8.2:+9491248 None:intergenic
ATCACATTCCCATTTGTTCT+TGG 0.308008 8.2:-9491959 MS.gene030201:CDS
GAGCAAGAGTAATTTGATAT+TGG 0.309795 8.2:-9489744 MS.gene030201:CDS
CCTGGAATGACATTGTCTTT+TGG 0.310244 8.2:-9489971 MS.gene030201:CDS
TTGTATACTGCCATCACTAT+TGG 0.316902 8.2:+9490691 None:intergenic
TGGACCTATTGGAAGCTATA+TGG 0.318011 8.2:-9489666 MS.gene030201:CDS
AGGGACAGGTTGTGCACCTT+TGG 0.336201 8.2:+9491901 None:intergenic
AGGACTGTTAAGCTTCTCTT+TGG 0.339704 8.2:+9492097 None:intergenic
GCCTTAGCAATTGCCCTTTG+TGG 0.342396 8.2:-9491983 MS.gene030201:CDS
GCTCTTGCCATATCATTATC+TGG 0.347069 8.2:-9491728 MS.gene030201:CDS
TGATATTCATCCTCTTGTTA+TGG 0.348410 8.2:-9489582 MS.gene030201:CDS
CACTCCACAATTGTTCATCT+TGG 0.352984 8.2:+9489864 None:intergenic
TATCTATGCAATGCACTTGA+TGG 0.358211 8.2:-9490506 MS.gene030201:CDS
AAGGTTGTTGTAATCTTCTT+CGG 0.366064 8.2:-9490085 MS.gene030201:CDS
GTCCCTTTCCTTGTCTTCAT+GGG 0.366810 8.2:-9491884 MS.gene030201:CDS
CAAATGTAAAGCTCTTTAAC+AGG 0.373112 8.2:+9491581 None:intergenic
TGATATTGAGACAGCATTGA+TGG 0.373365 8.2:-9489771 MS.gene030201:CDS
TGTTAATGATGTTGCAGCTT+GGG 0.380742 8.2:-9491759 MS.gene030201:CDS
GTTATCTTTACTGCTTGCTT+TGG 0.382323 8.2:-9491314 MS.gene030201:CDS
GATCATGAAGCACTTGCTTA+TGG 0.387350 8.2:-9490052 MS.gene030201:CDS
CACTAGTACAAATATCTTCA+TGG 0.402538 8.2:+9490305 None:intergenic
GTTGGTGTGTCTGGCACTTC+AGG 0.403487 8.2:+9489545 None:intergenic
TTCCTCGCTTTCGGGTTCCT+CGG 0.404658 8.2:+9490536 None:intergenic
TCCTCGCTTTCGGGTTCCTC+GGG 0.406728 8.2:+9490537 None:intergenic
GTTCCTATGCCAAGAACAAA+TGG 0.410938 8.2:+9491950 None:intergenic
AACTAACGTGTTGGTGTGTC+TGG 0.412667 8.2:+9489536 None:intergenic
CGTCGCCCCTGATTTCACTC+TGG 0.415870 8.2:-9491697 MS.gene030201:CDS
GCATAACCCAGAGTGAAATC+AGG 0.417320 8.2:+9491690 None:intergenic
CACTATTGGGGTGGTGATGA+AGG 0.425200 8.2:+9490705 None:intergenic
TTGTTCTTCTTATTCCTAGT+AGG 0.428237 8.2:-9492049 MS.gene030201:CDS
ACAACATGGAGTAAAATTCC+TGG 0.428599 8.2:+9490010 None:intergenic
TATAAGTGGAGGGGTTTCTT+GGG 0.428762 8.2:-9491351 MS.gene030201:CDS
CCAGCAAAAGGACCATCTTT+AGG 0.431587 8.2:+9491464 None:intergenic
TCTTTAACAGGTTCACCCTC+AGG 0.442719 8.2:+9491593 None:intergenic
TTACTAACCAATGGTGCAAC+CGG 0.444387 8.2:+9489707 None:intergenic
TGAAGAATCAATTAAGTATG+AGG 0.445267 8.2:-9489816 MS.gene030201:CDS
CTGAATCTTGATCTTTGCGT+TGG 0.445666 8.2:+9490629 None:intergenic
CCAAAAGACAATGTCATTCC+AGG 0.449037 8.2:+9489971 None:intergenic
CTGCTTGGGCCATCAGCAAT+AGG 0.449639 8.2:-9492151 MS.gene030201:CDS
CTGTTAATGATGTTGCAGCT+TGG 0.451241 8.2:-9491760 MS.gene030201:CDS
ACAAAATGATGAAGATCAAA+TGG 0.457736 8.2:-9490407 MS.gene030201:CDS
TCTCATGAACACCAAGGGAT+TGG 0.457954 8.2:-9491219 MS.gene030201:CDS
AATGCCACCGGTTGCACCAT+TGG 0.458140 8.2:-9489714 MS.gene030201:CDS
AACAGTGATTCAGCTGAACT+TGG 0.462781 8.2:-9489686 MS.gene030201:CDS
GGACTGTTAAGCTTCTCTTT+GGG 0.464658 8.2:+9492098 None:intergenic
GATGGAACCATGGAGTCATT+AGG 0.464824 8.2:-9490226 MS.gene030201:CDS
GTCGCCCCTGATTTCACTCT+GGG 0.466919 8.2:-9491696 MS.gene030201:CDS
TCGCTTCGACCTATTGCTGA+TGG 0.467719 8.2:+9492142 None:intergenic
AGTCATGCACCTTGTTCTGT+TGG 0.468441 8.2:-9490169 MS.gene030201:CDS
ATGCGTTCAATCCGTAGAAC+AGG 0.468555 8.2:-9492013 MS.gene030201:CDS
AAGACCGTTGCTTTCATACA+TGG 0.468874 8.2:-9491627 MS.gene030201:CDS
GTTATCGCTTTCCAAACCTA+TGG 0.474315 8.2:-9490385 MS.gene030201:CDS
TTTAGAGAAGCTCTTGCACT+TGG 0.475505 8.2:-9491245 MS.gene030201:CDS
TAAGGTGCTCCTCTTCGAGC+TGG 0.478801 8.2:+9490667 None:intergenic
GGTGCAGGACATGTCACATT+AGG 0.480678 8.2:+9492889 None:intergenic
GAAGCCATATAGCTTCCAAT+AGG 0.487421 8.2:+9489662 None:intergenic
GAACACAAATTGCAAATGCT+TGG 0.489528 8.2:+9490746 None:intergenic
AAGAGTAATTTGATATTGGT+TGG 0.496000 8.2:-9489740 MS.gene030201:CDS
ACAAATGTGGCTACTATAAG+TGG 0.497636 8.2:-9491365 MS.gene030201:CDS
AAGATTGAAGATCTTGTGTC+TGG 0.498090 8.2:-9491428 MS.gene030201:CDS
CAATGTTGGCTAGTGTGTCT+AGG 0.501202 8.2:+9492077 None:intergenic
TTGTGCACCTTTGGAGATTG+TGG 0.501442 8.2:+9491910 None:intergenic
TGGGAATGTGATTCCACAAA+GGG 0.501645 8.2:+9491970 None:intergenic
AGATTCAGAGCTCAGAGTCT+TGG 0.507048 8.2:-9490614 MS.gene030201:CDS
CCGTTAGACGTGGCCTTCAT+CGG 0.507520 8.2:+9492868 None:intergenic
CACCAAGATTGATGCAGAAG+TGG 0.507651 8.2:+9489628 None:intergenic
AGCTAGGATTCGAGCGAGGA+CGG 0.508099 8.2:+9491835 None:intergenic
CCGATGAAGGCCACGTCTAA+CGG 0.510024 8.2:-9492868 MS.gene030201:CDS
GACGTGGCCTTCATCGGTGC+AGG 0.510090 8.2:+9492874 None:intergenic
TCAATCAAAACACCAGCAAA+AGG 0.510996 8.2:+9491452 None:intergenic
TCCACAAAGGGCAATTGCTA+AGG 0.512792 8.2:+9491982 None:intergenic
GAAGAACAAAAGACCAATGT+TGG 0.513906 8.2:+9492063 None:intergenic
TGCAGGGAGGAATACTGCTT+GGG 0.514991 8.2:-9492165 MS.gene030201:intron
GTCGTCGCCAGATAATGATA+TGG 0.518435 8.2:+9491721 None:intergenic
CATTTGTATCACATTGACAT+TGG 0.519281 8.2:-9491564 MS.gene030201:CDS
TGTCGAGTTCAAGACCTACT+AGG 0.520539 8.2:+9492035 None:intergenic
ATGCAGGGAGGAATACTGCT+TGG 0.520657 8.2:-9492166 MS.gene030201:intron
GCTAGGATTCGAGCGAGGAC+GGG 0.524066 8.2:+9491836 None:intergenic
GGTATAATTATGCCTAAAGA+TGG 0.525746 8.2:-9491476 MS.gene030201:CDS
TTCCCATTTGTTCTTGGCAT+AGG 0.530710 8.2:-9491953 MS.gene030201:CDS
CTTTAACAGGTTCACCCTCA+GGG 0.537753 8.2:+9491594 None:intergenic
ATGAGAATGGCAGAACATCC+AGG 0.538151 8.2:-9490028 MS.gene030201:CDS
GAGAGCAGAAATTGCTGTCA+TGG 0.538200 8.2:+9490336 None:intergenic
GTAAAGTTCATTTCTCCACC+TGG 0.538553 8.2:-9489989 MS.gene030201:CDS
TTCCTATGCCAAGAACAAAT+GGG 0.538714 8.2:+9491951 None:intergenic
CTACAAGGTTGATTAGTGTA+GGG 0.540493 8.2:+9490566 None:intergenic
TCTACAAGGTTGATTAGTGT+AGG 0.546671 8.2:+9490565 None:intergenic
AAACTACTAACCACTGATGT+TGG 0.547854 8.2:-9491806 MS.gene030201:CDS
ACCAAAATTCCAACAGAACA+AGG 0.549877 8.2:+9490160 None:intergenic
AATTAAGTATGAGGAAAGGA+TGG 0.549955 8.2:-9489807 MS.gene030201:CDS
ACACCCATGAAGACAAGGAA+AGG 0.553825 8.2:+9491881 None:intergenic
GTATACTGCCATCACTATTG+GGG 0.558105 8.2:+9490693 None:intergenic
CACCCATGAAGACAAGGAAA+GGG 0.558235 8.2:+9491882 None:intergenic
CATAACCCAGAGTGAAATCA+GGG 0.561076 8.2:+9491691 None:intergenic
CATAAACACCAGCGTATCGA+TGG 0.563693 8.2:-9490244 MS.gene030201:CDS
ATACGCTGGTGTTTATGGAA+TGG 0.565041 8.2:+9490250 None:intergenic
CTCAGTATCAATGCTATGCA+AGG 0.565314 8.2:-9491273 MS.gene030201:CDS
GATATTGAAGGTAGCCATGA+TGG 0.569712 8.2:-9489896 MS.gene030201:CDS
ATGGCTAAGCGTTCCCCTGA+GGG 0.570360 8.2:-9491608 MS.gene030201:CDS
TGAATGTCCTAATGACTCCA+TGG 0.570671 8.2:+9490219 None:intergenic
AATGAGCTCAACCAATCCCT+TGG 0.575721 8.2:+9491208 None:intergenic
TGCATGAATTCCAATAGTAT+CGG 0.577236 8.2:+9491520 None:intergenic
AGAGCAGAAATTGCTGTCAT+GGG 0.578202 8.2:+9490337 None:intergenic
TCCAATTCCTCCATAACAAG+AGG 0.579300 8.2:+9489572 None:intergenic
ACTTGCTTATGGTATGAGAA+TGG 0.579368 8.2:-9490041 MS.gene030201:CDS
GTTGTTTGAGTGGTTTGCAA+AGG 0.579896 8.2:+9492752 None:intergenic
GTTGTGATTGATGATATTGA+AGG 0.581543 8.2:-9489908 MS.gene030201:CDS
TCTTTGGGAAAACTGTGTGT+AGG 0.583416 8.2:+9492113 None:intergenic
AAAACCAAGATGAACAATTG+TGG 0.583535 8.2:-9489868 MS.gene030201:CDS
TATTCATCCTCTTGTTATGG+AGG 0.583822 8.2:-9489579 MS.gene030201:CDS
GACATGTCCTGCACCGATGA+AGG 0.584379 8.2:-9492881 MS.gene030201:CDS
GCTGGTGTTTATGGAATGGA+AGG 0.586936 8.2:+9490254 None:intergenic
TCACTCTGGGTTATGCTCTG+TGG 0.589152 8.2:-9491683 MS.gene030201:CDS
GAGCAACACCCATGAAGACA+AGG 0.593494 8.2:+9491876 None:intergenic
GGATTTCTCATGAACACCAA+GGG 0.596547 8.2:-9491224 MS.gene030201:CDS
CTAGACACACTAGCCAACAT+TGG 0.598588 8.2:-9492076 MS.gene030201:CDS
ATGAAGACAAGGAAAGGGAC+AGG 0.600424 8.2:+9491887 None:intergenic
TAGTGGTTTGAAGACAAATG+TGG 0.600827 8.2:-9491378 MS.gene030201:CDS
ATGACTCGAGGTTGTTTGAG+TGG 0.603036 8.2:+9492742 None:intergenic
GAATCACTGTTACTAACCAA+TGG 0.603558 8.2:+9489698 None:intergenic
TCCCGAGGAACCCGAAAGCG+AGG 0.605796 8.2:-9490538 MS.gene030201:CDS
CCATGGTTCCATCGATACGC+TGG 0.609696 8.2:+9490236 None:intergenic
GAGCTCATTGTTCTCAACAT+CGG 0.610796 8.2:-9491194 MS.gene030201:CDS
AATCAATTAAGTATGAGGAA+AGG 0.611444 8.2:-9489811 MS.gene030201:CDS
TTAGTAGTTTGAGCTCAGCT+AGG 0.611873 8.2:+9491819 None:intergenic
GTTGTTGTAATCTTCTTCGG+AGG 0.614692 8.2:-9490082 MS.gene030201:CDS
TTGGTTGATCGCGGTCTCGG+AGG 0.615743 8.2:-9490142 MS.gene030201:CDS
ATTGGTTGGTAGAATGCCAC+CGG 0.617567 8.2:-9489726 MS.gene030201:CDS
CATCACCACCCCAATAGTGA+TGG 0.619210 8.2:-9490701 MS.gene030201:CDS
ATGATTTCTGCAATGACTCG+AGG 0.619902 8.2:+9492730 None:intergenic
TAGGGATGTTGCGTGTGCTG+TGG 0.621846 8.2:+9490584 None:intergenic
TTCCTCGGGATGATTCTACA+AGG 0.623083 8.2:+9490551 None:intergenic
ATGTGGCTACTATAAGTGGA+GGG 0.627903 8.2:-9491361 MS.gene030201:CDS
CTTAGAGCCACAATCTCCAA+AGG 0.629193 8.2:-9491917 MS.gene030201:CDS
TGTGGCTACTATAAGTGGAG+GGG 0.631044 8.2:-9491360 MS.gene030201:CDS
GTTGCGTGCTTTGTGAACCA+TGG 0.633464 8.2:+9490456 None:intergenic
CCACTAGAAGCAAAATAGAG+TGG 0.635208 8.2:+9491395 None:intergenic
GTTAATTCTTCAGATATGTG+TGG 0.635681 8.2:-9492803 MS.gene030201:CDS
AATGTGGCTACTATAAGTGG+AGG 0.637657 8.2:-9491362 MS.gene030201:CDS
AACGGAAGTGCATAATCGAG+AGG 0.638478 8.2:+9492826 None:intergenic
TTGGAATGCACCGTTAGACG+TGG 0.639960 8.2:+9492858 None:intergenic
CCAGCGTATCGATGGAACCA+TGG 0.641905 8.2:-9490236 MS.gene030201:CDS
TGGATTTCTCATGAACACCA+AGG 0.642435 8.2:-9491225 MS.gene030201:CDS
GTTCACAAAGCACGCAACAA+TGG 0.642568 8.2:-9490451 MS.gene030201:CDS
TGCTATACGACCAACATCAG+TGG 0.645855 8.2:+9491796 None:intergenic
ATGGGAATGTGATTCCACAA+AGG 0.646197 8.2:+9491969 None:intergenic
CATTGTTCTCAACATCGGAA+AGG 0.647673 8.2:-9491189 MS.gene030201:CDS
CAACATCGGAAAGGATCGCA+AGG 0.652219 8.2:-9491180 MS.gene030201:intron
CATGGCTAAGCGTTCCCCTG+AGG 0.653527 8.2:-9491609 MS.gene030201:CDS
CTAACCAATGGTGCAACCGG+TGG 0.654366 8.2:+9489710 None:intergenic
TTTAACAGGTTCACCCTCAG+GGG 0.666338 8.2:+9491595 None:intergenic
TTAGCCATGTATGAAAGCAA+CGG 0.666589 8.2:+9491623 None:intergenic
TACTGCCATCACTATTGGGG+TGG 0.669299 8.2:+9490696 None:intergenic
GAGAAATGAACTTTACAACA+TGG 0.677164 8.2:+9489996 None:intergenic
CATATCTGAAGAATTAACAA+CGG 0.681401 8.2:+9492808 None:intergenic
AAAGACAATGTCATTCCAGG+TGG 0.682922 8.2:+9489974 None:intergenic
GTATACAAACCAGCTCGAAG+AGG 0.689387 8.2:-9490676 MS.gene030201:CDS
GATTGTGGCTCTAAGAACAA+CGG 0.700703 8.2:+9491925 None:intergenic
GAATGGAAGGAGTATTATCG+CGG 0.711496 8.2:+9490267 None:intergenic
AACCTTGTAGAATCATCCCG+AGG 0.713913 8.2:-9490553 MS.gene030201:CDS
AAGATCTTCAGCTATCACCA+TGG 0.719007 8.2:-9490473 MS.gene030201:CDS
GCTCAGCTAGGATTCGAGCG+AGG 0.755526 8.2:+9491831 None:intergenic
ATAACCCAGAGTGAAATCAG+GGG 0.767323 8.2:+9491692 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATTAATCTTATAAAG+TGG - chr8.2:9489900-9489919 MS.gene030201:CDS 10.0%
!! AAAATATAACCATCAAATTA+AGG + chr8.2:9489808-9489827 None:intergenic 15.0%
!! AAATATAACCATCAAATTAA+GGG + chr8.2:9489807-9489826 None:intergenic 15.0%
!! TCAGAAAAGAATTAAAATAT+CGG - chr8.2:9491420-9491439 MS.gene030201:CDS 15.0%
!! TTGAAATATTTAACGATAAT+TGG + chr8.2:9491604-9491623 None:intergenic 15.0%
!!! ATTTTGTCATTAATTATTAG+AGG + chr8.2:9491548-9491567 None:intergenic 15.0%
!!! GTTTTATATTAAGATATAAG+TGG - chr8.2:9490151-9490170 MS.gene030201:CDS 15.0%
!! CAGAAAAGAATTAAAATATC+GGG - chr8.2:9491421-9491440 MS.gene030201:CDS 20.0%
!! TAATTATTAGAGGATCAATA+AGG + chr8.2:9491538-9491557 None:intergenic 20.0%
!! TTTCAAATCTTTCATTACTT+TGG - chr8.2:9491618-9491637 MS.gene030201:CDS 20.0%
!! TTTGATAAAACAACAAATTG+TGG + chr8.2:9489778-9489797 None:intergenic 20.0%
!!! ATTTTGTTTTTTGTTCCAAA+AGG + chr8.2:9492021-9492040 None:intergenic 20.0%
!!! TGTTTTGTTTTGTAAATTAG+TGG - chr8.2:9490045-9490064 MS.gene030201:CDS 20.0%
!!! TTTTGTTTTTTGTTCCAAAA+GGG + chr8.2:9492020-9492039 None:intergenic 20.0%
! AAATTGATATGTAAGCTAGT+CGG + chr8.2:9490024-9490043 None:intergenic 25.0%
! AACAAAATTGCACTGAAATA+TGG + chr8.2:9491394-9491413 None:intergenic 25.0%
! AAGAGTAATTTGATATTGGT+TGG - chr8.2:9492701-9492720 MS.gene030201:intron 25.0%
! AATCAATTAAGTATGAGGAA+AGG - chr8.2:9492630-9492649 MS.gene030201:intron 25.0%
! ACAAAATGATGAAGATCAAA+TGG - chr8.2:9492034-9492053 MS.gene030201:CDS 25.0%
! ATTGGAATTCATGCATTATT+TGG - chr8.2:9490929-9490948 MS.gene030201:intron 25.0%
! ATTTGCAATTTGTGTTCTTA+TGG - chr8.2:9491701-9491720 MS.gene030201:CDS 25.0%
! CATATCTGAAGAATTAACAA+CGG + chr8.2:9489636-9489655 None:intergenic 25.0%
! TGAAGAATCAATTAAGTATG+AGG - chr8.2:9492625-9492644 MS.gene030201:intron 25.0%
! TGGACTAATGATACAAAATA+GGG + chr8.2:9489758-9489777 None:intergenic 25.0%
!! CGATGTTGGTTATTATATTA+AGG - chr8.2:9490095-9490114 MS.gene030201:CDS 25.0%
!!! ACCTTTTGAATTGATTTTGA+GGG - chr8.2:9489990-9490009 MS.gene030201:CDS 25.0%
!!! TACCTTTTGAATTGATTTTG+AGG - chr8.2:9489989-9490008 MS.gene030201:CDS 25.0%
!!! TTATTTGGTGCTTTTGTTAT+TGG - chr8.2:9490944-9490963 MS.gene030201:intron 25.0%
!!! TTTATTTTCTTCCATATGCA+CGG + chr8.2:9489951-9489970 None:intergenic 25.0%
AAAACCAAGATGAACAATTG+TGG - chr8.2:9492573-9492592 MS.gene030201:intron 30.0%
AAGGTTGTTGTAATCTTCTT+CGG - chr8.2:9492356-9492375 MS.gene030201:intron 30.0%
AATTAAGTATGAGGAAAGGA+TGG - chr8.2:9492634-9492653 MS.gene030201:intron 30.0%
AGTTAGTGAGACAATGAATA+AGG - chr8.2:9489850-9489869 MS.gene030201:CDS 30.0%
ATGTGAAAATGTTCAATGCA+GGG - chr8.2:9490260-9490279 MS.gene030201:CDS 30.0%
CAAATGTAAAGCTCTTTAAC+AGG + chr8.2:9490863-9490882 None:intergenic 30.0%
CACTAGTACAAATATCTTCA+TGG + chr8.2:9492139-9492158 None:intergenic 30.0%
CATTTGTATCACATTGACAT+TGG - chr8.2:9490877-9490896 MS.gene030201:intron 30.0%
GAAGATTACAACAACCTTAA+AGG + chr8.2:9492354-9492373 None:intergenic 30.0%
GAGAAATGAACTTTACAACA+TGG + chr8.2:9492448-9492467 None:intergenic 30.0%
GAGCAAGAGTAATTTGATAT+TGG - chr8.2:9492697-9492716 MS.gene030201:intron 30.0%
GGACTAATGATACAAAATAG+GGG + chr8.2:9489757-9489776 None:intergenic 30.0%
GGATACTTCTAAGTATTACT+TGG + chr8.2:9491583-9491602 None:intergenic 30.0%
GGTATAATTATGCCTAAAGA+TGG - chr8.2:9490965-9490984 MS.gene030201:intron 30.0%
GTTAATTCTTCAGATATGTG+TGG - chr8.2:9489638-9489657 MS.gene030201:CDS 30.0%
TATGTGAAAATGTTCAATGC+AGG - chr8.2:9490259-9490278 MS.gene030201:CDS 30.0%
TCCCTCAAAATCAATTCAAA+AGG + chr8.2:9489994-9490013 None:intergenic 30.0%
TGATATTCATCCTCTTGTTA+TGG - chr8.2:9492859-9492878 MS.gene030201:CDS 30.0%
TGCATGAATTCCAATAGTAT+CGG + chr8.2:9490924-9490943 None:intergenic 30.0%
TTGTTCTTCTTATTCCTAGT+AGG - chr8.2:9490392-9490411 MS.gene030201:CDS 30.0%
! AAGAAAGTCCCTTAATTTGA+TGG - chr8.2:9489796-9489815 MS.gene030201:CDS 30.0%
! GTGGACTAATGATACAAAAT+AGG + chr8.2:9489759-9489778 None:intergenic 30.0%
! GTTGTGATTGATGATATTGA+AGG - chr8.2:9492533-9492552 MS.gene030201:intron 30.0%
! TGGACTGTTTTGTGTTTATA+AGG + chr8.2:9491795-9491814 None:intergenic 30.0%
! TGTTCAATTTTCCATAGGTT+TGG + chr8.2:9492070-9492089 None:intergenic 30.0%
! TTTGAAATCAAACGATGATG+TGG + chr8.2:9489927-9489946 None:intergenic 30.0%
!!! AGTTTTGTTACCGATACTAT+TGG - chr8.2:9490911-9490930 MS.gene030201:intron 30.0%
!!! ATTGTCTTTTGGTGCTAAAT+TGG - chr8.2:9492481-9492500 MS.gene030201:intron 30.0%
!!! TATCACATTGACATTGGTTT+TGG - chr8.2:9490883-9490902 MS.gene030201:intron 30.0%
AAACTACTAACCACTGATGT+TGG - chr8.2:9490635-9490654 MS.gene030201:CDS 35.0%
AAGATTGAAGATCTTGTGTC+TGG - chr8.2:9491013-9491032 MS.gene030201:intron 35.0%
ACAAATGTGGCTACTATAAG+TGG - chr8.2:9491076-9491095 MS.gene030201:intron 35.0%
ACAACATGGAGTAAAATTCC+TGG + chr8.2:9492434-9492453 None:intergenic 35.0%
ACCAAAATTCCAACAGAACA+AGG + chr8.2:9492284-9492303 None:intergenic 35.0%
ACTTGCTTATGGTATGAGAA+TGG - chr8.2:9492400-9492419 MS.gene030201:intron 35.0%
AGCAAGTGAAGTTTCCTTTA+AGG - chr8.2:9492337-9492356 MS.gene030201:intron 35.0%
ATCACATTCCCATTTGTTCT+TGG - chr8.2:9490482-9490501 MS.gene030201:CDS 35.0%
CATTTAGTACCTGTGTTACA+TGG + chr8.2:9491674-9491693 None:intergenic 35.0%
GAACACAAATTGCAAATGCT+TGG + chr8.2:9491698-9491717 None:intergenic 35.0%
GAAGAACAAAAGACCAATGT+TGG + chr8.2:9490381-9490400 None:intergenic 35.0%
GAATCACTGTTACTAACCAA+TGG + chr8.2:9492746-9492765 None:intergenic 35.0%
GTTATCTTTACTGCTTGCTT+TGG - chr8.2:9491127-9491146 MS.gene030201:intron 35.0%
TAGTGGTTTGAAGACAAATG+TGG - chr8.2:9491063-9491082 MS.gene030201:intron 35.0%
TATCTATGCAATGCACTTGA+TGG - chr8.2:9491935-9491954 MS.gene030201:CDS 35.0%
TCAATCAAAACACCAGCAAA+AGG + chr8.2:9490992-9491011 None:intergenic 35.0%
TGATTTCAAATCCGTGCATA+TGG - chr8.2:9489937-9489956 MS.gene030201:CDS 35.0%
TGTAATTCTCCATGTAACAC+AGG - chr8.2:9491662-9491681 MS.gene030201:CDS 35.0%
TGTATACTGCCATCACTATT+GGG + chr8.2:9491752-9491771 None:intergenic 35.0%
TGTCTCACTAACTTATTGCA+TGG + chr8.2:9489843-9489862 None:intergenic 35.0%
TGTTAATGATGTTGCAGCTT+GGG - chr8.2:9490682-9490701 MS.gene030201:CDS 35.0%
TTAGCCATGTATGAAAGCAA+CGG + chr8.2:9490821-9490840 None:intergenic 35.0%
TTCCTATGCCAAGAACAAAT+GGG + chr8.2:9490493-9490512 None:intergenic 35.0%
TTGTATACTGCCATCACTAT+TGG + chr8.2:9491753-9491772 None:intergenic 35.0%
! ACCTTGTTCTGTTGGAATTT+TGG - chr8.2:9492280-9492299 MS.gene030201:intron 35.0%
! CTACAAGGTTGATTAGTGTA+GGG + chr8.2:9491878-9491897 None:intergenic 35.0%
! GACACTGTTCAATTTTCCAT+AGG + chr8.2:9492075-9492094 None:intergenic 35.0%
! TATTCATCCTCTTGTTATGG+AGG - chr8.2:9492862-9492881 MS.gene030201:CDS 35.0%
! TCTACAAGGTTGATTAGTGT+AGG + chr8.2:9491879-9491898 None:intergenic 35.0%
! TGATATTGAGACAGCATTGA+TGG - chr8.2:9492670-9492689 MS.gene030201:intron 35.0%
!! GCTTGCTTTGGAAAAATTGT+TGG - chr8.2:9491139-9491158 MS.gene030201:intron 35.0%
!!! AGGCTTTTTTACCTGTTCTA+CGG + chr8.2:9490442-9490461 None:intergenic 35.0%
!!! TCTTTTGGTGCTAAATTGGT+TGG - chr8.2:9492485-9492504 MS.gene030201:intron 35.0%
AAAGACAATGTCATTCCAGG+TGG + chr8.2:9492470-9492489 None:intergenic 40.0%
AACAGTGATTCAGCTGAACT+TGG - chr8.2:9492755-9492774 MS.gene030201:CDS 40.0%
AAGATCTTCAGCTATCACCA+TGG - chr8.2:9491968-9491987 MS.gene030201:CDS 40.0%
AAGTGACAGCAACATTCAAC+AGG + chr8.2:9490227-9490246 None:intergenic 40.0%
AATGTGGCTACTATAAGTGG+AGG - chr8.2:9491079-9491098 MS.gene030201:intron 40.0%
AGAGCAGAAATTGCTGTCAT+GGG + chr8.2:9492107-9492126 None:intergenic 40.0%
AGGACTGTTAAGCTTCTCTT+TGG + chr8.2:9490347-9490366 None:intergenic 40.0%
AGTGCAAGAGCTTCTCTAAA+AGG + chr8.2:9491196-9491215 None:intergenic 40.0%
ATAACCCAGAGTGAAATCAG+GGG + chr8.2:9490752-9490771 None:intergenic 40.0%
ATACGCTGGTGTTTATGGAA+TGG + chr8.2:9492194-9492213 None:intergenic 40.0%
ATGATTTCTGCAATGACTCG+AGG + chr8.2:9489714-9489733 None:intergenic 40.0%
ATGGGAATGTGATTCCACAA+AGG + chr8.2:9490475-9490494 None:intergenic 40.0%
ATGTGGCTACTATAAGTGGA+GGG - chr8.2:9491080-9491099 MS.gene030201:intron 40.0%
CACTCCACAATTGTTCATCT+TGG + chr8.2:9492580-9492599 None:intergenic 40.0%
CATAACCCAGAGTGAAATCA+GGG + chr8.2:9490753-9490772 None:intergenic 40.0%
CATTCATGCTCTTTGCACTA+TGG + chr8.2:9492607-9492626 None:intergenic 40.0%
CATTGTTCTCAACATCGGAA+AGG - chr8.2:9491252-9491271 MS.gene030201:CDS 40.0%
CCAAAAGACAATGTCATTCC+AGG + chr8.2:9492473-9492492 None:intergenic 40.0%
CCACTAGAAGCAAAATAGAG+TGG + chr8.2:9491049-9491068 None:intergenic 40.0%
CGATCGAAATCTGGATAGAT+AGG + chr8.2:9491301-9491320 None:intergenic 40.0%
CTCAGTATCAATGCTATGCA+AGG - chr8.2:9491168-9491187 MS.gene030201:intron 40.0%
CTGAATCTTGATCTTTGCGT+TGG + chr8.2:9491815-9491834 None:intergenic 40.0%
CTGTTAATGATGTTGCAGCT+TGG - chr8.2:9490681-9490700 MS.gene030201:CDS 40.0%
GAAGCCATATAGCTTCCAAT+AGG + chr8.2:9492782-9492801 None:intergenic 40.0%
GAATAATCGTGCATCTACAC+AGG - chr8.2:9490067-9490086 MS.gene030201:CDS 40.0%
GACTATAAGCGATCGAAATC+TGG + chr8.2:9491310-9491329 None:intergenic 40.0%
GAGCTCATTGTTCTCAACAT+CGG - chr8.2:9491247-9491266 MS.gene030201:CDS 40.0%
GATATTGAAGGTAGCCATGA+TGG - chr8.2:9492545-9492564 MS.gene030201:intron 40.0%
GCATTTGGATCGAAATCAGA+CGG + chr8.2:9491460-9491479 None:intergenic 40.0%
GCTCTTGCCATATCATTATC+TGG - chr8.2:9490713-9490732 MS.gene030201:CDS 40.0%
GGACTGTTAAGCTTCTCTTT+GGG + chr8.2:9490346-9490365 None:intergenic 40.0%
GGATAGATAGGAAGACAGAT+AGG + chr8.2:9491289-9491308 None:intergenic 40.0%
GGATTTCTCATGAACACCAA+GGG - chr8.2:9491217-9491236 MS.gene030201:CDS 40.0%
GTAAAGTTCATTTCTCCACC+TGG - chr8.2:9492452-9492471 MS.gene030201:intron 40.0%
GTATACTGCCATCACTATTG+GGG + chr8.2:9491751-9491770 None:intergenic 40.0%
GTCAGTACATTAGTGGCATT+TGG + chr8.2:9491475-9491494 None:intergenic 40.0%
GTTATCGCTTTCCAAACCTA+TGG - chr8.2:9492056-9492075 MS.gene030201:CDS 40.0%
GTTCCTATGCCAAGAACAAA+TGG + chr8.2:9490494-9490513 None:intergenic 40.0%
GTTGTTGTAATCTTCTTCGG+AGG - chr8.2:9492359-9492378 MS.gene030201:intron 40.0%
TAAGGCAGTCAGTACATTAG+TGG + chr8.2:9491482-9491501 None:intergenic 40.0%
TACAAAGTCTTCCTCGCTTT+CGG + chr8.2:9491917-9491936 None:intergenic 40.0%
TATAAGTGGAGGGGTTTCTT+GGG - chr8.2:9491090-9491109 MS.gene030201:intron 40.0%
TCAGCAATCTCAAATCTCCA+TGG - chr8.2:9491328-9491347 MS.gene030201:CDS 40.0%
TCCAATTCCTCCATAACAAG+AGG + chr8.2:9492872-9492891 None:intergenic 40.0%
TGAAAATGTTCAATGCAGGG+AGG - chr8.2:9490263-9490282 MS.gene030201:CDS 40.0%
TGAATGTCCTAATGACTCCA+TGG + chr8.2:9492225-9492244 None:intergenic 40.0%
TGGACCTATTGGAAGCTATA+TGG - chr8.2:9492775-9492794 MS.gene030201:CDS 40.0%
TGGATTTCTCATGAACACCA+AGG - chr8.2:9491216-9491235 MS.gene030201:CDS 40.0%
TGGGAATGTGATTCCACAAA+GGG + chr8.2:9490474-9490493 None:intergenic 40.0%
TTACTAACCAATGGTGCAAC+CGG + chr8.2:9492737-9492756 None:intergenic 40.0%
TTAGTAGTTTGAGCTCAGCT+AGG + chr8.2:9490625-9490644 None:intergenic 40.0%
TTCCCATTTGTTCTTGGCAT+AGG - chr8.2:9490488-9490507 MS.gene030201:CDS 40.0%
TTTAGAGAAGCTCTTGCACT+TGG - chr8.2:9491196-9491215 MS.gene030201:CDS 40.0%
! AAGACCGTTGCTTTCATACA+TGG - chr8.2:9490814-9490833 MS.gene030201:intron 40.0%
! AATCGAGAGGATTTTCGTGT+TGG + chr8.2:9489605-9489624 None:intergenic 40.0%
! CCACTCTATTTTGCTTCTAG+TGG - chr8.2:9491046-9491065 MS.gene030201:intron 40.0%
! CCTGGAATGACATTGTCTTT+TGG - chr8.2:9492470-9492489 MS.gene030201:intron 40.0%
! GATCATGAAGCACTTGCTTA+TGG - chr8.2:9492389-9492408 MS.gene030201:intron 40.0%
! GTTGTTTGAGTGGTTTGCAA+AGG + chr8.2:9489692-9489711 None:intergenic 40.0%
! TCCTCTTGTTATGGAGGAAT+TGG - chr8.2:9492868-9492887 MS.gene030201:CDS 40.0%
! TCTTTGGGAAAACTGTGTGT+AGG + chr8.2:9490331-9490350 None:intergenic 40.0%
!! CTTGGTTTTTACTGCCATCA+TGG + chr8.2:9492562-9492581 None:intergenic 40.0%
!! GAATGGAAGGAGTATTATCG+CGG + chr8.2:9492177-9492196 None:intergenic 40.0%
!! GATTGTGGCTCTAAGAACAA+CGG + chr8.2:9490519-9490538 None:intergenic 40.0%
!!! GTTGGAATTTTGGTTGATCG+CGG - chr8.2:9492290-9492309 MS.gene030201:intron 40.0%
AACCTTGTAGAATCATCCCG+AGG - chr8.2:9491888-9491907 MS.gene030201:CDS 45.0%
AATGAGCTCAACCAATCCCT+TGG + chr8.2:9491236-9491255 None:intergenic 45.0%
ACAAAGTCTTCCTCGCTTTC+GGG + chr8.2:9491916-9491935 None:intergenic 45.0%
ACACCCATGAAGACAAGGAA+AGG + chr8.2:9490563-9490582 None:intergenic 45.0%
AGATTCAGAGCTCAGAGTCT+TGG - chr8.2:9491827-9491846 MS.gene030201:CDS 45.0%
AGCTAGCAATCCACGCATTA+AGG + chr8.2:9491500-9491519 None:intergenic 45.0%
AGTCATGCACCTTGTTCTGT+TGG - chr8.2:9492272-9492291 MS.gene030201:intron 45.0%
ATGAAGACAAGGAAAGGGAC+AGG + chr8.2:9490557-9490576 None:intergenic 45.0%
ATGACTCGAGGTTGTTTGAG+TGG + chr8.2:9489702-9489721 None:intergenic 45.0%
ATGAGAATGGCAGAACATCC+AGG - chr8.2:9492413-9492432 MS.gene030201:intron 45.0%
ATGCGTTCAATCCGTAGAAC+AGG - chr8.2:9490428-9490447 MS.gene030201:CDS 45.0%
CACCAAGATTGATGCAGAAG+TGG + chr8.2:9492816-9492835 None:intergenic 45.0%
CACCCATGAAGACAAGGAAA+GGG + chr8.2:9490562-9490581 None:intergenic 45.0%
CATAAACACCAGCGTATCGA+TGG - chr8.2:9492197-9492216 MS.gene030201:intron 45.0%
CATCGATACGCTGGTGTTTA+TGG + chr8.2:9492199-9492218 None:intergenic 45.0%
CCAGCAAAAGGACCATCTTT+AGG + chr8.2:9490980-9490999 None:intergenic 45.0%
CTAGACACACTAGCCAACAT+TGG - chr8.2:9490365-9490384 MS.gene030201:CDS 45.0%
CTATAAGTGGAGGGGTTTCT+TGG - chr8.2:9491089-9491108 MS.gene030201:intron 45.0%
CTCCACTTCTGCATCAATCT+TGG - chr8.2:9492811-9492830 MS.gene030201:CDS 45.0%
CTTAGAGCCACAATCTCCAA+AGG - chr8.2:9490524-9490543 MS.gene030201:CDS 45.0%
CTTTAACAGGTTCACCCTCA+GGG + chr8.2:9490850-9490869 None:intergenic 45.0%
GAGAGCAGAAATTGCTGTCA+TGG + chr8.2:9492108-9492127 None:intergenic 45.0%
GATGGAACCATGGAGTCATT+AGG - chr8.2:9492215-9492234 MS.gene030201:intron 45.0%
GCATAACCCAGAGTGAAATC+AGG + chr8.2:9490754-9490773 None:intergenic 45.0%
GCTGGTGTTTATGGAATGGA+AGG + chr8.2:9492190-9492209 None:intergenic 45.0%
GTATACAAACCAGCTCGAAG+AGG - chr8.2:9491765-9491784 MS.gene030201:CDS 45.0%
GTCCCTTTCCTTGTCTTCAT+GGG - chr8.2:9490557-9490576 MS.gene030201:CDS 45.0%
GTCGTCGCCAGATAATGATA+TGG + chr8.2:9490723-9490742 None:intergenic 45.0%
GTTCACAAAGCACGCAACAA+TGG - chr8.2:9491990-9492009 MS.gene030201:CDS 45.0%
TCAGCTGAACTTGGACCTAT+TGG - chr8.2:9492764-9492783 MS.gene030201:CDS 45.0%
TCCACAAAGGGCAATTGCTA+AGG + chr8.2:9490462-9490481 None:intergenic 45.0%
TCTCATGAACACCAAGGGAT+TGG - chr8.2:9491222-9491241 MS.gene030201:CDS 45.0%
TCTTTAACAGGTTCACCCTC+AGG + chr8.2:9490851-9490870 None:intergenic 45.0%
TGCTATACGACCAACATCAG+TGG + chr8.2:9490648-9490667 None:intergenic 45.0%
TGTCCCTTTCCTTGTCTTCA+TGG - chr8.2:9490556-9490575 MS.gene030201:CDS 45.0%
TGTCGAGTTCAAGACCTACT+AGG + chr8.2:9490409-9490428 None:intergenic 45.0%
TGTGGCTACTATAAGTGGAG+GGG - chr8.2:9491081-9491100 MS.gene030201:intron 45.0%
TTCCTCGGGATGATTCTACA+AGG + chr8.2:9491893-9491912 None:intergenic 45.0%
TTTAACAGGTTCACCCTCAG+GGG + chr8.2:9490849-9490868 None:intergenic 45.0%
! AACGGAAGTGCATAATCGAG+AGG + chr8.2:9489618-9489637 None:intergenic 45.0%
! CAATGTTGGCTAGTGTGTCT+AGG + chr8.2:9490367-9490386 None:intergenic 45.0%
! CCTAAAGATGGTCCTTTTGC+TGG - chr8.2:9490977-9490996 MS.gene030201:intron 45.0%
! GCAACAATGGAATGCCCTTT+TGG - chr8.2:9492003-9492022 MS.gene030201:CDS 45.0%
! TTGTGCACCTTTGGAGATTG+TGG + chr8.2:9490534-9490553 None:intergenic 45.0%
!! ATTGGTTGGTAGAATGCCAC+CGG - chr8.2:9492715-9492734 MS.gene030201:intron 45.0%
!!! ATTTTGGTTGATCGCGGTCT+CGG - chr8.2:9492296-9492315 MS.gene030201:intron 45.0%
ATGCAGGGAGGAATACTGCT+TGG - chr8.2:9490275-9490294 MS.gene030201:CDS 50.0%
CAACATCGGAAAGGATCGCA+AGG - chr8.2:9491261-9491280 MS.gene030201:CDS 50.0%
CATCACCACCCCAATAGTGA+TGG - chr8.2:9491740-9491759 MS.gene030201:CDS 50.0%
CTACACAGGTCTTGCGATGT+TGG - chr8.2:9490081-9490100 MS.gene030201:CDS 50.0%
GAGCAACACCCATGAAGACA+AGG + chr8.2:9490568-9490587 None:intergenic 50.0%
GCCTTAGCAATTGCCCTTTG+TGG - chr8.2:9490458-9490477 MS.gene030201:CDS 50.0%
GGTGCAGGACATGTCACATT+AGG + chr8.2:9489555-9489574 None:intergenic 50.0%
GTTGCGTGCTTTGTGAACCA+TGG + chr8.2:9491988-9492007 None:intergenic 50.0%
TACTGCCATCACTATTGGGG+TGG + chr8.2:9491748-9491767 None:intergenic 50.0%
TCACTCTGGGTTATGCTCTG+TGG - chr8.2:9490758-9490777 MS.gene030201:CDS 50.0%
TCGCTTCGACCTATTGCTGA+TGG + chr8.2:9490302-9490321 None:intergenic 50.0%
TGCAGGGAGGAATACTGCTT+GGG - chr8.2:9490276-9490295 MS.gene030201:CDS 50.0%
TTGGAATGCACCGTTAGACG+TGG + chr8.2:9489586-9489605 None:intergenic 50.0%
! CACTATTGGGGTGGTGATGA+AGG + chr8.2:9491739-9491758 None:intergenic 50.0%
!! GTACTGACTGCCTTAATGCG+TGG - chr8.2:9491487-9491506 MS.gene030201:CDS 50.0%
!! TCACTGATGTGCTGACTCCA+TGG + chr8.2:9491348-9491367 None:intergenic 50.0%
AGCTAGGATTCGAGCGAGGA+CGG + chr8.2:9490609-9490628 None:intergenic 55.0%
AGGGACAGGTTGTGCACCTT+TGG + chr8.2:9490543-9490562 None:intergenic 55.0%
ATGGCTAAGCGTTCCCCTGA+GGG - chr8.2:9490833-9490852 MS.gene030201:intron 55.0%
CCATGGTTCCATCGATACGC+TGG + chr8.2:9492208-9492227 None:intergenic 55.0%
CCGATGAAGGCCACGTCTAA+CGG - chr8.2:9489573-9489592 MS.gene030201:CDS 55.0%
CCGTTAGACGTGGCCTTCAT+CGG + chr8.2:9489576-9489595 None:intergenic 55.0%
CTAACCAATGGTGCAACCGG+TGG + chr8.2:9492734-9492753 None:intergenic 55.0%
CTGCTTGGGCCATCAGCAAT+AGG - chr8.2:9490290-9490309 MS.gene030201:CDS 55.0%
GTCGCCCCTGATTTCACTCT+GGG - chr8.2:9490745-9490764 MS.gene030201:CDS 55.0%
GTTGGTGTGTCTGGCACTTC+AGG + chr8.2:9492899-9492918 None:intergenic 55.0%
TAGGGATGTTGCGTGTGCTG+TGG + chr8.2:9491860-9491879 None:intergenic 55.0%
! AATGCCACCGGTTGCACCAT+TGG - chr8.2:9492727-9492746 MS.gene030201:intron 55.0%
! CCAGCGTATCGATGGAACCA+TGG - chr8.2:9492205-9492224 MS.gene030201:intron 55.0%
! GACATGTCCTGCACCGATGA+AGG - chr8.2:9489560-9489579 MS.gene030201:CDS 55.0%
! TAAGGTGCTCCTCTTCGAGC+TGG + chr8.2:9491777-9491796 None:intergenic 55.0%
!! TTCCTCGCTTTCGGGTTCCT+CGG + chr8.2:9491908-9491927 None:intergenic 55.0%
CATGGCTAAGCGTTCCCCTG+AGG - chr8.2:9490832-9490851 MS.gene030201:intron 60.0%
CGTCGCCCCTGATTTCACTC+TGG - chr8.2:9490744-9490763 MS.gene030201:CDS 60.0%
GCTAGGATTCGAGCGAGGAC+GGG + chr8.2:9490608-9490627 None:intergenic 60.0%
GCTCAGCTAGGATTCGAGCG+AGG + chr8.2:9490613-9490632 None:intergenic 60.0%
TTGGTTGATCGCGGTCTCGG+AGG - chr8.2:9492299-9492318 MS.gene030201:intron 60.0%
!! TCCTCGCTTTCGGGTTCCTC+GGG + chr8.2:9491907-9491926 None:intergenic 60.0%
GACGTGGCCTTCATCGGTGC+AGG + chr8.2:9489570-9489589 None:intergenic 65.0%
TCCCGAGGAACCCGAAAGCG+AGG - chr8.2:9491903-9491922 MS.gene030201:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 9489543 9492920 9489543 ID=MS.gene030201
chr8.2 mRNA 9489543 9492920 9489543 ID=MS.gene030201.t1;Parent=MS.gene030201
chr8.2 exon 9492729 9492920 9492729 ID=MS.gene030201.t1.exon1;Parent=MS.gene030201.t1
chr8.2 CDS 9492729 9492920 9492729 ID=cds.MS.gene030201.t1;Parent=MS.gene030201.t1
chr8.2 exon 9491181 9492182 9491181 ID=MS.gene030201.t1.exon2;Parent=MS.gene030201.t1
chr8.2 CDS 9491181 9492182 9491181 ID=cds.MS.gene030201.t1;Parent=MS.gene030201.t1
chr8.2 exon 9489543 9490779 9489543 ID=MS.gene030201.t1.exon3;Parent=MS.gene030201.t1
chr8.2 CDS 9489543 9490779 9489543 ID=cds.MS.gene030201.t1;Parent=MS.gene030201.t1
Gene Sequence

>MS.gene030201

ATGGCGACGCCTAATGTGACATGTCCTGCACCGATGAAGGCCACGTCTAACGGTGCATTCCAACACGAAAATCCTCTCGATTATGCACTTCCGTTGTTAATTCTTCAGATATGTGTGGTTGTCACATTCACTAGATGTATTGCATACCTTTGCAAACCACTCAAACAACCTCGAGTCATTGCAGAAATCATTGTAAGTTGTCTTCTCTTTTCCCCTATTTTGTATCATTAGTCCACAATTTGTTGTTTTATCAAAGAAAGTCCCTTAATTTGATGGTTATATTTTATATTTGAGTTTCCATGCAATAAGTTAGTGAGACAATGAATAAGGCAGTGTGACATGATTGACAAATTATTATAATTATTAATCTTATAAAGTGGTCCACATCATCGTTTGATTTCAAATCCGTGCATATGGAAGAAAATAAAAATTGTCAATGAGAAAATTACCTTTTGAATTGATTTTGAGGGATTAGTCTCCGACTAGCTTACATATCAATTTTTGTTTTGTTTTGTAAATTAGTGGAATAATCGTGCATCTACACAGGTCTTGCGATGTTGGTTATTATATTAAGGTAGTTAATATAAGATTAAAATGTTTAGAATTTTGTTTTATATTAAGATATAAGTGGAATTATTGAAACGTAATTTCTTCTGTTGCAAGTAAAACTTTTGTATGTTGCCTGTTGAATGTTGCTGTCACTTTTGTAAGTTTCTTATGTGAAAATGTTCAATGCAGGGAGGAATACTGCTTGGGCCATCAGCAATAGGTCGAAGCGAGAAATTCCTACACACAGTTTTCCCAAAGAGAAGCTTAACAGTCCTAGACACACTAGCCAACATTGGTCTTTTGTTCTTCTTATTCCTAGTAGGTCTTGAACTCGACATGCGTTCAATCCGTAGAACAGGTAAAAAAGCCTTAGCAATTGCCCTTTGTGGAATCACATTCCCATTTGTTCTTGGCATAGGAACATCCGTTGTTCTTAGAGCCACAATCTCCAAAGGTGCACAACCTGTCCCTTTCCTTGTCTTCATGGGTGTTGCTCTCTCAATCACAGCATTTCCCGTCCTCGCTCGAATCCTAGCTGAGCTCAAACTACTAACCACTGATGTTGGTCGTATAGCAATGTCAGCAGCTGCTGTTAATGATGTTGCAGCTTGGGTACTTCTTGCTCTTGCCATATCATTATCTGGCGACGACACGTCGCCCCTGATTTCACTCTGGGTTATGCTCTGTGGTGCTGCTTTTATTCTCTTTGCTGTTTTTGCTATAAGACCGTTGCTTTCATACATGGCTAAGCGTTCCCCTGAGGGTGAACCTGTTAAAGAGCTTTACATTTGTATCACATTGACATTGGTTTTGGCTTGTAGTTTTGTTACCGATACTATTGGAATTCATGCATTATTTGGTGCTTTTGTTATTGGTATAATTATGCCTAAAGATGGTCCTTTTGCTGGTGTTTTGATTGAGAAGATTGAAGATCTTGTGTCTGGAATCTTTTTGCCACTCTATTTTGCTTCTAGTGGTTTGAAGACAAATGTGGCTACTATAAGTGGAGGGGTTTCTTGGGCACTATTATTACTAGTTATCTTTACTGCTTGCTTTGGAAAAATTGTTGGTACAATCTCAGTATCAATGCTATGCAAGGTGCCTTTTAGAGAAGCTCTTGCACTTGGATTTCTCATGAACACCAAGGGATTGGTTGAGCTCATTGTTCTCAACATCGGAAAGGATCGCAAGGTACCTATCTGTCTTCCTATCTATCCAGATTTCGATCGCTTATAGTCAGCAATCTCAAATCTCCATGGAGTCAGCACATCAGTGACTTTTCGATCGAGATCAGACTATCCATATTTCAGTGCAATTTTGTTGATTTTTCAGAAAAGAATTAAAATATCGGGTCTGAAGTTTGAACCGTCTGATTTCGATCCAAATGCCACTAATGTACTGACTGCCTTAATGCGTGGATTGCTAGCTGCAGACAATCTAAATCCTTATTGATCCTCTAATAATTAATGACAAAATAAAACTTGTTTTCCAAGTAATACTTAGAAGTATCCAATTATCGTTAAATATTTCAAATCTTTCATTACTTTGGTTTGATTTTTTATGCATGTAATGTAATTCTCCATGTAACACAGGTACTAAATGACCAAGCATTTGCAATTTGTGTTCTTATGGCATTATTCACTACCTTCATCACCACCCCAATAGTGATGGCAGTATACAAACCAGCTCGAAGAGGAGCACCTTATAAACACAAAACAGTCCAACGCAAAGATCAAGATTCAGAGCTCAGAGTCTTGGCATGTTTCCACAGCACACGCAACATCCCTACACTAATCAACCTTGTAGAATCATCCCGAGGAACCCGAAAGCGAGGAAGACTTTGTATCTATGCAATGCACTTGATGGAACTCTCAGAAAGATCTTCAGCTATCACCATGGTTCACAAAGCACGCAACAATGGAATGCCCTTTTGGAACAAAAAACAAAATGATGAAGATCAAATGGTTATCGCTTTCCAAACCTATGGAAAATTGAACAGTGTCAATGTTCGTCCCATGACAGCAATTTCTGCTCTCAGCAACATCCATGAAGATATTTGTACTAGTGCTCATCAAAAGCGCGCCGCGATAATACTCCTTCCATTCCATAAACACCAGCGTATCGATGGAACCATGGAGTCATTAGGACATTCATTTCATGTAATCAATGAGCTTGTGTTGAGTCATGCACCTTGTTCTGTTGGAATTTTGGTTGATCGCGGTCTCGGAGGAACAAGTCAAGTTCAAGCAAGTGAAGTTTCCTTTAAGGTTGTTGTAATCTTCTTCGGAGGACGCGACGATCATGAAGCACTTGCTTATGGTATGAGAATGGCAGAACATCCAGGAATTTTACTCCATGTTGTAAAGTTCATTTCTCCACCTGGAATGACATTGTCTTTTGGTGCTAAATTGGTTGGTGTAGCACAAGATAAGAGCAAAACAGTTGTGATTGATGATATTGAAGGTAGCCATGATGGCAGTAAAAACCAAGATGAACAATTGTGGAGTGAATTCCATAGTGCAAAGAGCATGAATGAAGAATCAATTAAGTATGAGGAAAGGATGGTTGAAAGTAAAAATGATATTGAGACAGCATTGATGGAATTGAGCAAGAGTAATTTGATATTGGTTGGTAGAATGCCACCGGTTGCACCATTGGTTAGTAACAGTGATTCAGCTGAACTTGGACCTATTGGAAGCTATATGGCTTCTTCTAGTTTCTCCACTTCTGCATCAATCTTGGTGATTCAACAATATAATTCAACAACTGATATTCATCCTCTTGTTATGGAGGAATTGGATTGTCCTGAAGTGCCAGACACACCAACAC
Protein sequence

>MS.gene030201.t1

MATPNVTCPAPMKATSNGAFQHENPLDYALPLLILQICVVVTFTRCIAYLCKPLKQPRVIAEIIGGILLGPSAIGRSEKFLHTVFPKRSLTVLDTLANIGLLFFLFLVGLELDMRSIRRTGKKALAIALCGITFPFVLGIGTSVVLRATISKGAQPVPFLVFMGVALSITAFPVLARILAELKLLTTDVGRIAMSAAAVNDVAAWVLLALAISLSGDDTSPLISLWVMLCGAAFILFAVFAIRPLLSYMAKRSPEGEPVKELYICITLTLVLACSFVTDTIGIHALFGAFVIGIIMPKDGPFAGVLIEKIEDLVSGIFLPLYFASSGLKTNVATISGGVSWALLLLVIFTACFGKIVGTISVSMLCKVPFREALALGFLMNTKGLVELIVLNIGKDRKVLNDQAFAICVLMALFTTFITTPIVMAVYKPARRGAPYKHKTVQRKDQDSELRVLACFHSTRNIPTLINLVESSRGTRKRGRLCIYAMHLMELSERSSAITMVHKARNNGMPFWNKKQNDEDQMVIAFQTYGKLNSVNVRPMTAISALSNIHEDICTSAHQKRAAIILLPFHKHQRIDGTMESLGHSFHVINELVLSHAPCSVGILVDRGLGGTSQVQASEVSFKVVVIFFGGRDDHEALAYGMRMAEHPGILLHVVKFISPPGMTLSFGAKLVGVAQDKSKTVVIDDIEGSHDGSKNQDEQLWSEFHSAKSMNEESIKYEERMVESKNDIETALMELSKSNLILVGRMPPVAPLVSNSDSAELGPIGSYMASSSFSTSASILVIQQYNSTTDIHPLVMEELDCPEVPDTPT