AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031733

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031733.t1 MTR_4g078120 97.311 781 21 0 1 781 1 781 0.0 1553
MS.gene031733.t1 MTR_4g123583 79.481 770 157 1 1 770 1 769 0.0 1243
MS.gene031733.t1 MTR_5g009780 61.548 788 282 4 3 785 6 777 0.0 974
MS.gene031733.t1 MTR_5g009770 61.529 785 285 5 3 785 6 775 0.0 971
MS.gene031733.t1 MTR_5g009800 58.101 790 292 7 3 788 6 760 0.0 912
MS.gene031733.t1 MTR_5g009770 60.546 659 243 5 129 785 1 644 0.0 794
MS.gene031733.t1 MTR_8g093780 45.908 782 406 11 8 781 13 785 0.0 699
MS.gene031733.t1 MTR_3g096380 45.844 770 400 9 8 770 14 773 0.0 698
MS.gene031733.t1 MTR_7g099800 50.366 820 336 17 12 780 8 807 0.0 664
MS.gene031733.t1 MTR_2g011520 43.669 774 411 10 15 781 3 758 0.0 652
MS.gene031733.t1 MTR_7g099820 48.228 790 356 16 13 773 5 770 0.0 646
MS.gene031733.t1 MTR_7g099820 53.092 663 286 9 13 658 5 659 0.0 636
MS.gene031733.t1 MTR_7g099750 48.526 814 339 20 12 773 8 793 0.0 634
MS.gene031733.t1 MTR_3g031170 32.600 773 476 11 8 772 29 764 1.53e-131 410
MS.gene031733.t1 MTR_5g067690 32.250 800 498 13 3 781 10 786 2.27e-123 390
MS.gene031733.t1 MTR_5g074380 31.538 780 479 18 16 778 76 817 8.54e-119 379
MS.gene031733.t1 MTR_3g037270 31.154 780 498 16 20 781 25 783 2.82e-118 376
MS.gene031733.t1 MTR_5g074360 30.691 782 483 17 16 778 31 772 3.69e-118 376
MS.gene031733.t1 MTR_3g071990 29.040 792 515 13 8 788 12 767 8.64e-118 375
MS.gene031733.t1 MTR_2g040100 31.447 795 486 19 20 781 36 804 7.85e-117 373
MS.gene031733.t1 MTR_3g037260 31.202 782 497 16 20 781 21 781 1.01e-116 372
MS.gene031733.t1 MTR_1g043400 30.923 802 504 16 6 781 2 779 2.48e-116 371
MS.gene031733.t1 MTR_5g074340 30.591 778 493 16 15 778 30 774 3.22e-116 371
MS.gene031733.t1 MTR_0002s1180 31.043 786 502 14 15 781 16 780 7.41e-116 370
MS.gene031733.t1 MTR_1g045870 30.836 801 491 17 6 781 2 764 4.25e-112 359
MS.gene031733.t1 MTR_3g031160 28.750 800 506 18 8 787 24 779 6.34e-106 343
MS.gene031733.t1 MTR_5g074320 31.276 729 450 17 65 778 10 702 1.11e-104 338
MS.gene031733.t1 MTR_3g071990 29.526 718 459 13 82 788 4 685 2.33e-104 337
MS.gene031733.t1 MTR_3g071880 28.900 782 508 20 18 775 22 779 2.49e-99 326
MS.gene031733.t1 MTR_3g498715 29.602 804 479 20 11 781 38 787 3.23e-98 323
MS.gene031733.t1 MTR_6g015940 28.394 803 503 16 6 783 27 782 8.53e-95 313
MS.gene031733.t1 MTR_3g071870 28.518 796 493 19 16 775 33 788 2.73e-94 313
MS.gene031733.t1 MTR_4g037755 29.233 756 482 17 49 781 38 763 4.60e-93 308
MS.gene031733.t1 MTR_8g085260 28.055 802 509 16 6 783 29 786 1.57e-91 305
MS.gene031733.t1 MTR_6g053320 29.183 747 441 19 80 788 71 767 8.40e-89 297
MS.gene031733.t1 MTR_4g029520 27.690 762 522 17 35 781 25 772 1.96e-87 293
MS.gene031733.t1 MTR_3g031030 29.489 763 471 22 47 781 36 759 1.24e-86 291
MS.gene031733.t1 MTR_3g110700 27.191 776 526 15 24 781 15 769 2.38e-85 288
MS.gene031733.t1 MTR_5g088470 26.817 798 517 21 9 781 28 783 1.87e-84 286
MS.gene031733.t1 MTR_3g083850 26.933 802 505 19 11 781 28 779 3.76e-83 282
MS.gene031733.t1 MTR_5g088450 27.586 812 502 24 6 781 25 786 3.41e-82 280
MS.gene031733.t1 MTR_7g085870 26.158 799 509 20 13 772 24 780 3.65e-81 277
MS.gene031733.t1 MTR_5g088430 26.467 801 514 20 11 781 28 783 1.16e-80 275
MS.gene031733.t1 MTR_5g088790 26.600 797 527 14 11 781 45 809 5.74e-78 268
MS.gene031733.t1 MTR_7g053000 25.847 797 533 14 11 781 45 809 4.09e-75 261
MS.gene031733.t1 MTR_7g052680 25.972 797 532 14 11 781 45 809 1.32e-74 259
MS.gene031733.t1 MTR_7g052970 25.847 797 533 14 11 781 45 809 3.42e-74 258
MS.gene031733.t1 MTR_5g088840 25.280 803 531 17 11 781 45 810 3.79e-72 252
MS.gene031733.t1 MTR_1g082340 95.876 97 4 0 452 548 82 178 1.58e-59 202
MS.gene031733.t1 MTR_5g061460 90.099 101 10 0 448 548 11 111 2.72e-57 193
MS.gene031733.t1 MTR_3g465680 30.534 393 265 6 17 404 44 433 2.74e-50 191
MS.gene031733.t1 MTR_3g465680 27.475 404 225 15 398 781 555 910 2.77e-35 144
MS.gene031733.t1 MTR_7g052895 24.074 540 387 8 11 532 45 579 7.51e-44 170
MS.gene031733.t1 MTR_1g023830 95.918 49 2 0 500 548 1 49 3.10e-28 108
MS.gene031733.t1 MTR_5g083560 30.741 270 150 8 522 781 51 293 6.53e-25 107
MS.gene031733.t1 MTR_3g083860 25.339 221 127 7 10 225 28 215 2.95e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031733.t1 AT3G17630 69.845 776 217 3 8 783 10 768 0.0 1072
MS.gene031733.t1 AT3G17630 69.845 776 217 3 8 783 10 768 0.0 1072
MS.gene031733.t1 AT5G41610 61.568 791 286 5 1 783 1 781 0.0 947
MS.gene031733.t1 AT5G41610 61.568 791 286 5 1 783 1 781 0.0 947
MS.gene031733.t1 AT4G23700 59.748 795 300 10 1 781 1 789 0.0 923
MS.gene031733.t1 AT4G23700 59.748 795 300 10 1 781 1 789 0.0 923
MS.gene031733.t1 AT5G41610 61.134 723 264 4 68 783 1 713 0.0 855
MS.gene031733.t1 AT1G64170 52.244 802 334 13 4 781 10 786 0.0 769
MS.gene031733.t1 AT2G13620 48.912 781 380 10 8 781 15 783 0.0 709
MS.gene031733.t1 AT3G53720 51.311 801 344 17 12 773 8 801 0.0 678
MS.gene031733.t1 AT3G53720 50.341 733 318 17 80 773 3 728 0.0 585
MS.gene031733.t1 AT1G05580 33.725 765 474 10 29 781 50 793 3.75e-134 419
MS.gene031733.t1 AT1G05580 34.046 702 430 10 92 781 2 682 1.27e-121 384
MS.gene031733.t1 AT2G31910 31.004 787 488 13 19 781 26 781 9.71e-117 373
MS.gene031733.t1 AT1G06970 31.586 782 487 14 8 775 28 775 1.09e-114 367
MS.gene031733.t1 AT2G31910 31.513 714 434 13 92 781 2 684 1.82e-104 338
MS.gene031733.t1 AT2G31910 31.513 714 434 13 92 781 2 684 1.82e-104 338
MS.gene031733.t1 AT2G30240 30.446 785 491 16 8 775 30 776 2.94e-100 329
MS.gene031733.t1 AT5G37060 27.284 799 519 13 18 781 53 824 2.38e-88 298
MS.gene031733.t1 AT5G01690 27.925 795 503 19 8 781 17 762 1.32e-87 293
MS.gene031733.t1 AT5G58460 27.227 797 520 13 18 781 53 822 9.13e-87 293
MS.gene031733.t1 AT5G22900 26.545 825 513 19 11 781 27 812 3.26e-82 280
MS.gene031733.t1 AT3G44900 26.811 787 518 16 27 783 51 809 5.67e-81 276
MS.gene031733.t1 AT3G52080 28.097 783 510 20 27 781 22 779 2.13e-77 266
MS.gene031733.t1 AT1G79400 26.974 760 529 13 24 773 17 760 6.26e-77 265
MS.gene031733.t1 AT2G28180 27.778 792 501 21 27 788 60 810 1.03e-76 265
MS.gene031733.t1 AT5G01680 26.408 799 520 21 8 781 15 770 2.80e-75 260
MS.gene031733.t1 AT1G08150 27.448 776 496 20 27 775 62 797 4.77e-73 255
MS.gene031733.t1 AT1G16380 24.651 787 550 16 24 791 17 779 9.51e-72 251
MS.gene031733.t1 AT3G44910 26.701 779 486 18 28 777 35 757 4.88e-71 248
MS.gene031733.t1 AT5G22910 26.010 792 505 19 21 775 37 784 1.06e-69 245
MS.gene031733.t1 AT3G44930 25.163 767 525 13 28 777 35 769 8.50e-65 231
MS.gene031733.t1 AT2G28170 25.191 786 515 18 27 783 54 795 6.47e-60 218
MS.gene031733.t1 AT1G08140 25.286 787 520 22 24 781 51 798 2.31e-58 213
MS.gene031733.t1 AT3G44920 25.823 790 503 17 28 783 35 775 2.98e-54 201
MS.gene031733.t1 AT1G08135 24.020 791 511 21 27 783 57 791 2.58e-47 181

Find 177 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGCTCGGTTGGAATTT+TGG 0.066676 4.3:+30941612 MS.gene031733:CDS
CTTCCCCTCTTGTTTCTCTT+TGG 0.133782 4.3:+30939954 MS.gene031733:CDS
CATCACTTTGACACTAGTTT+TGG 0.158755 4.3:+30940093 MS.gene031733:CDS
GTAATTGCATTTCAAGCTTA+TGG 0.179873 4.3:+30941388 MS.gene031733:CDS
TATAGCAAGTAAAGGCTTTA+TGG 0.181728 4.3:-30940020 None:intergenic
GCTCTTGCAATAGCTCTTTC+TGG 0.198455 4.3:+30939923 MS.gene031733:CDS
ACATATCTGTACAATTAATA+AGG 0.206129 4.3:-30938976 None:intergenic
GAATAAGAGCAATCTGATTT+TGG 0.226807 4.3:+30942029 MS.gene031733:CDS
AGCTGTTATACCAATCAATT+TGG 0.229645 4.3:-30941827 None:intergenic
CCAATGAAGGCCACTTCTAA+TGG 0.246725 4.3:+30938915 MS.gene031733:CDS
GAAATAATGGCATGCCCTTT+TGG 0.250974 4.3:+30941332 MS.gene031733:CDS
AACAAGAAGATGATCGATTT+TGG 0.257022 4.3:+30941905 MS.gene031733:CDS
TTCCCCTCTTGTTTCTCTTT+GGG 0.258305 4.3:+30939955 MS.gene031733:CDS
ATTGGTATTCATGCCCTCTT+TGG 0.275653 4.3:+30940139 MS.gene031733:CDS
TGGTCCTGTTGGAAGCTATT+TGG 0.282128 4.3:+30942107 MS.gene031733:CDS
AAAGCCTTTACTTGCTATAA+TGG 0.296096 4.3:+30940024 MS.gene031733:CDS
TGATTCTATTAGATTGATTA+AGG 0.297215 4.3:-30941209 None:intergenic
TCTTCTTGTTTATCTTCATC+TGG 0.325379 4.3:-30941892 None:intergenic
TATTAGTGGAGCACTTTCTT+GGG 0.331925 4.3:+30940300 MS.gene031733:CDS
CATAAACACCAACGAGTTGA+TGG 0.341640 4.3:+30941529 MS.gene031733:CDS
GTTCATAAGGCAAGAAATAA+TGG 0.341864 4.3:+30941319 MS.gene031733:CDS
CTATTAGTGGAGCACTTTCT+TGG 0.342232 4.3:+30940299 MS.gene031733:CDS
GAATCATCGCAACTCGCTTT+TGG 0.342247 4.3:-30941495 None:intergenic
TTATACACTGCCACCACTAT+TGG 0.350992 4.3:-30941079 None:intergenic
GCCTTATGCATAGCCCTTGC+TGG 0.353372 4.3:+30939668 MS.gene031733:CDS
TGCCTTATGAACCATTGTTA+TGG 0.361294 4.3:-30941308 None:intergenic
AGGGTCAAAGCTTCTCTAAA+AGG 0.366872 4.3:-30940403 None:intergenic
CAATGAAGGCCACTTCTAAT+GGG 0.367620 4.3:+30938916 MS.gene031733:CDS
AATGTTGTTGTAGCTTTCTT+CGG 0.368301 4.3:+30941688 MS.gene031733:CDS
CTTGAAATGCAATTACCATT+TGG 0.373268 4.3:-30941381 None:intergenic
CCATTAGAAGTGGCCTTCAT+TGG 0.373450 4.3:-30938915 None:intergenic
CATATATGCAATGCACTTGA+TGG 0.376324 4.3:+30941264 MS.gene031733:CDS
GTTATATTTACTGCTTGCTT+TGG 0.381877 4.3:+30940337 MS.gene031733:CDS
ATCACATTCCCATTTGTACT+TGG 0.392656 4.3:+30939692 MS.gene031733:CDS
ACTCGTTGGTGTTTATGAAA+TGG 0.395464 4.3:-30941523 None:intergenic
CCGGGTAAAACATTGGCATT+TGG 0.398158 4.3:+30941802 MS.gene031733:CDS
TTTAGAGAAGCTTTGACCCT+TGG 0.398592 4.3:+30940406 MS.gene031733:CDS
CCAGTAGCACCTTTGCTAGA+TGG 0.399351 4.3:+30942066 MS.gene031733:CDS
TGGGTCCTACTTTGTGGTGT+TGG 0.409022 4.3:+30939974 MS.gene031733:CDS
GTGCCAATGCCAAGTACAAA+TGG 0.411262 4.3:-30939701 None:intergenic
TCCTTCACAGGTTCACCCTC+AGG 0.411786 4.3:-30940058 None:intergenic
CCAACGAGTTGATGGAACTA+TGG 0.413496 4.3:+30941537 MS.gene031733:CDS
TTACTAGGACCCTCAGCAAT+TGG 0.414653 4.3:+30939500 MS.gene031733:CDS
ATTAATTGTACAGATATGTT+TGG 0.419171 4.3:+30938980 MS.gene031733:CDS
CCTCAGCAATTGGGAGGAAC+AGG 0.419963 4.3:+30939510 MS.gene031733:CDS
CCATCTAGCAAAGGTGCTAC+TGG 0.420352 4.3:-30942066 None:intergenic
CTGTTCCTCCCAATTGCTGA+GGG 0.426519 4.3:-30939509 None:intergenic
ACTAATGTAGCTACTATTAG+TGG 0.426835 4.3:+30940286 MS.gene031733:CDS
GATGGAACTATGGAATCATT+AGG 0.430780 4.3:+30941547 MS.gene031733:CDS
GCTTGCTTTGGAAAAGTTGT+TGG 0.431269 4.3:+30940349 MS.gene031733:CDS
ATGCGTTCGATACGCAAAAC+AGG 0.432662 4.3:+30939638 MS.gene031733:CDS
CTAGAAATAAGAAGAATAGA+AGG 0.436879 4.3:-30939598 None:intergenic
GATTAAGGTAGGAATGTTGT+AGG 0.437399 4.3:-30941194 None:intergenic
AAAACAGTGAGTGAAATTCC+AGG 0.441950 4.3:-30941763 None:intergenic
AGCCATAACAATGGTTCATA+AGG 0.442015 4.3:+30941306 MS.gene031733:CDS
TGAAGAATCTATAGTTTATG+AGG 0.445781 4.3:+30941957 MS.gene031733:CDS
CTATTCTTCTTATTTCTAGT+AGG 0.446847 4.3:+30939602 MS.gene031733:CDS
AGAGATGAAATTGCTGTCAT+AGG 0.447748 4.3:-30941436 None:intergenic
TCTCTTTGGGTCCTACTTTG+TGG 0.448654 4.3:+30939968 MS.gene031733:CDS
TGTGCTGAGCTTGGTCCTGT+TGG 0.449228 4.3:+30942096 MS.gene031733:CDS
ATTAACATCTTTGTTTATTG+TGG 0.452653 4.3:-30939741 None:intergenic
GGCATTGTTGTTCCTAAGGA+TGG 0.454690 4.3:+30940175 MS.gene031733:CDS
GCATTTGGTGCCAAATTGAT+TGG 0.454735 4.3:+30941817 MS.gene031733:CDS
CATATCTGTACAATTAATAA+GGG 0.456561 4.3:-30938975 None:intergenic
TATGGTGATCCTCTCCTAGC+TGG 0.459919 4.3:-30941103 None:intergenic
TATAGTTTATGAGGAAAGGT+TGG 0.460120 4.3:+30941966 MS.gene031733:CDS
AGCTGGCATTGTTGTTCCTA+AGG 0.464868 4.3:+30940171 MS.gene031733:CDS
AGTATCCTAAGCTCTGTGTC+TGG 0.465681 4.3:-30941154 None:intergenic
CACTCATTTCGCGTAATGAA+TGG 0.465697 4.3:+30941571 MS.gene031733:CDS
TTTAAAATTTATAACTCCGC+CGG 0.470716 4.3:+30941783 MS.gene031733:CDS
TTAGAAGTGGCCTTCATTGG+TGG 0.474237 4.3:-30938912 None:intergenic
TTGTAACTGTGTTGAGGGAA+AGG 0.475546 4.3:+30942007 MS.gene031733:CDS
CCTGTTCCTCCCAATTGCTG+AGG 0.475723 4.3:-30939510 None:intergenic
TTTCATCACCACACCAATAG+TGG 0.476008 4.3:+30941066 MS.gene031733:CDS
TTGGTTGATCGCGGCCTTGG+AGG 0.476860 4.3:+30941631 MS.gene031733:CDS
TCCAGCAAGGGCTATGCATA+AGG 0.479938 4.3:-30939669 None:intergenic
AATGCTAATAAGTGCACTTA+GGG 0.480045 4.3:-30942813 None:intergenic
ACTTAGCCACGCTCCTTGCT+CGG 0.480167 4.3:+30941600 MS.gene031733:CDS
AGATATTGTAACTGTGTTGA+GGG 0.481278 4.3:+30942002 MS.gene031733:CDS
ACTTGGCATTGGCACCTCAC+TGG 0.481962 4.3:+30939709 MS.gene031733:CDS
TACTAGGACCCTCAGCAATT+GGG 0.484048 4.3:+30939501 MS.gene031733:CDS
TGGTTGATCGCGGCCTTGGA+GGG 0.485004 4.3:+30941632 MS.gene031733:CDS
AAATGCTAATAAGTGCACTT+AGG 0.488035 4.3:-30942814 None:intergenic
TCTCATGAATACCAAGGGAT+TGG 0.488737 4.3:+30940432 MS.gene031733:CDS
ATTTGCAATATGTGTTGTAA+TGG 0.490511 4.3:+30941030 MS.gene031733:CDS
CTGTCAATGATGTTGCTGCT+TGG 0.491013 4.3:+30939891 MS.gene031733:CDS
GAAGCCAAATAGCTTCCAAC+AGG 0.493769 4.3:-30942111 None:intergenic
TTCCCATTTGTACTTGGCAT+TGG 0.494111 4.3:+30939698 MS.gene031733:CDS
AATTGTACAGATATGTTTGG+TGG 0.496042 4.3:+30938983 MS.gene031733:CDS
ATGAAAACATGGAAGGCTGC+TGG 0.497137 4.3:-30939764 None:intergenic
TTAGCCATTATAGCAAGTAA+AGG 0.499517 4.3:-30940028 None:intergenic
ACATGACAACAAAGACCAAA+TGG 0.502030 4.3:+30941366 MS.gene031733:CDS
CATGAATACCAAGGGATTGG+TGG 0.503412 4.3:+30940435 MS.gene031733:CDS
TGAACCATTGTTATGGCTGA+AGG 0.506107 4.3:-30941301 None:intergenic
TCTATTAGATTGATTAAGGT+AGG 0.507378 4.3:-30941205 None:intergenic
TAAGGGAAGTGCATAATCCA+TGG 0.509239 4.3:-30938958 None:intergenic
ACCAAAATTCCAACCGAGCA+AGG 0.509906 4.3:-30941613 None:intergenic
AATCTATAGTTTATGAGGAA+AGG 0.511325 4.3:+30941962 MS.gene031733:CDS
TCAGTCAAAACACCAGCAAA+AGG 0.511406 4.3:-30940199 None:intergenic
AACATGTCCACCACCAATGA+AGG 0.511745 4.3:+30938902 MS.gene031733:CDS
AAGATCCAGACACAGAGCTT+AGG 0.515149 4.3:+30941149 MS.gene031733:CDS
CCAGCAAAAGGACCATCCTT+AGG 0.517602 4.3:-30940187 None:intergenic
CACAACCGATGTTGGTCGTA+TGG 0.517934 4.3:+30939853 MS.gene031733:CDS
AGCCACGCTCCTTGCTCGGT+TGG 0.520274 4.3:+30941604 MS.gene031733:CDS
CCACTAGACACAAAATAAAG+TGG 0.521769 4.3:-30940256 None:intergenic
GAAGTGGCCTTCATTGGTGG+TGG 0.529547 4.3:-30938909 None:intergenic
TTAAAATTTATAACTCCGCC+GGG 0.531879 4.3:+30941784 MS.gene031733:CDS
TGCCAATGCCAAGTACAAAT+GGG 0.532684 4.3:-30939700 None:intergenic
ATGGCTAAACGTTGCCCTGA+GGG 0.532790 4.3:+30940043 MS.gene031733:CDS
AAGATATTGTAACTGTGTTG+AGG 0.533146 4.3:+30942001 MS.gene031733:CDS
CAAATGTAAATCTCCTTCAC+AGG 0.535425 4.3:-30940070 None:intergenic
AAACTTCTCACAACCGATGT+TGG 0.539573 4.3:+30939845 MS.gene031733:CDS
ATGCAGGGAGGAATATTACT+AGG 0.549080 4.3:+30939485 MS.gene031733:intron
ATGACTTCTGCAATAACTCT+AGG 0.550504 4.3:-30939053 None:intergenic
AATGAGCTCCACCAATCCCT+TGG 0.552682 4.3:-30940443 None:intergenic
ATGAGAATAGCAGAACATCC+TGG 0.554571 4.3:+30941745 MS.gene031733:CDS
GGTGGTGGACATGTTGAACC+AGG 0.555124 4.3:-30938894 None:intergenic
GCTAGGATTCGAGCAAGGAC+AGG 0.555675 4.3:-30939815 None:intergenic
GGATTTCTCATGAATACCAA+GGG 0.560991 4.3:+30940427 MS.gene031733:CDS
TGAGAAGTTTGAGTTCAGCT+AGG 0.563629 4.3:-30939832 None:intergenic
CTTGATACACTAGCCAACAT+TGG 0.566464 4.3:+30939575 MS.gene031733:CDS
TAAAACCAACACCACAAAGT+AGG 0.567534 4.3:-30939979 None:intergenic
GGACTTGAGTTGTCCCTCCA+AGG 0.568272 4.3:-30941645 None:intergenic
GATCGCGAAGCACTTGCGTA+CGG 0.570816 4.3:+30941721 MS.gene031733:CDS
ATTAGTGGAGCACTTTCTTG+GGG 0.572192 4.3:+30940301 MS.gene031733:CDS
TGGATTTCTCATGAATACCA+AGG 0.573049 4.3:+30940426 MS.gene031733:CDS
AACTCCGCCGGGTAAAACAT+TGG 0.573285 4.3:+30941795 MS.gene031733:CDS
AGAAACAAGAGGGGAAGTGT+TGG 0.573411 4.3:-30939948 None:intergenic
CCATAGTTCCATCAACTCGT+TGG 0.579409 4.3:-30941537 None:intergenic
CCCTGAGGGTGAACCTGTGA+AGG 0.581418 4.3:+30940057 MS.gene031733:CDS
GTTGTTGTAGCTTTCTTCGG+AGG 0.582576 4.3:+30941691 MS.gene031733:CDS
GAAGAATAGAAGGCCAATGT+TGG 0.582577 4.3:-30939588 None:intergenic
GGAAGTGATTGTGCTGAGCT+TGG 0.585556 4.3:+30942087 MS.gene031733:CDS
TGTAAGAAACATCACTTGCA+TGG 0.585634 4.3:-30941666 None:intergenic
CCTTCACAGGTTCACCCTCA+GGG 0.588249 4.3:-30940057 None:intergenic
GGACCCAAAGAGAAACAAGA+GGG 0.589200 4.3:-30939958 None:intergenic
TTGAAATGCCCCATTAGAAG+TGG 0.589578 4.3:-30938925 None:intergenic
GAGCAACGCCCATGAAAACA+TGG 0.592836 4.3:-30939775 None:intergenic
GATTCGAGCAAGGACAGGAA+AGG 0.596316 4.3:-30939810 None:intergenic
AAAGTAATAAAAGTGTCAGA+TGG 0.596338 4.3:+30941862 MS.gene031733:CDS
TGGGAATGTGATTCCAGCAA+GGG 0.596813 4.3:-30939681 None:intergenic
AGCTACAAAAGCTCCAAAGA+GGG 0.598339 4.3:-30940152 None:intergenic
CAACATCGGCAAGGATCGCA+AGG 0.599446 4.3:+30940471 MS.gene031733:CDS
AAGGGAAGTGCATAATCCAT+GGG 0.601133 4.3:-30938957 None:intergenic
TAGGAATGTTGTAGGTGCTG+TGG 0.603732 4.3:-30941186 None:intergenic
AAATTGAAAATGGCTACCCC+TGG 0.608511 4.3:+30938876 None:intergenic
TGGTATTCATGAGAAATCCA+AGG 0.617918 4.3:-30940423 None:intergenic
AATCTAATAGAATCATCAAG+AGG 0.619281 4.3:+30941217 MS.gene031733:CDS
CATTGCCATACGACCAACAT+CGG 0.622192 4.3:-30939858 None:intergenic
ATTTCAACATGAAAATCCCA+TGG 0.624328 4.3:+30938941 MS.gene031733:CDS
CAGCTACAAAAGCTCCAAAG+AGG 0.627516 4.3:-30940153 None:intergenic
GTGTATAAACCAGCTAGGAG+AGG 0.627924 4.3:+30941094 MS.gene031733:CDS
ATGGGAATGTGATTCCAGCA+AGG 0.628568 4.3:-30939682 None:intergenic
TGGCAGTGTATAAACCAGCT+AGG 0.630922 4.3:+30941089 MS.gene031733:CDS
TCAAGAGGAACACGAAAGCG+AGG 0.639084 4.3:+30941232 MS.gene031733:CDS
AATATGTGTTGTAATGGCAC+TGG 0.641607 4.3:+30941036 MS.gene031733:CDS
AGGACCCAAAGAGAAACAAG+AGG 0.642100 4.3:-30939959 None:intergenic
CAATCACTTCCATCTAGCAA+AGG 0.642844 4.3:-30942075 None:intergenic
AACGCCCATGAAAACATGGA+AGG 0.646352 4.3:-30939771 None:intergenic
CACTGCCACCACTATTGGTG+TGG 0.650997 4.3:-30941074 None:intergenic
TCTAGCAAAGGTGCTACTGG+AGG 0.652663 4.3:-30942063 None:intergenic
GGTATTCATGAGAAATCCAA+GGG 0.653988 4.3:-30940422 None:intergenic
GAGCTCATTGTTCTCAACAT+CGG 0.654678 4.3:+30940457 MS.gene031733:CDS
AGCAAGCAGTAAATATAACA+AGG 0.654704 4.3:-30940333 None:intergenic
GTTCAGCTAGGATTCGAGCA+AGG 0.657730 4.3:-30939820 None:intergenic
TAGGACCCTCAGCAATTGGG+AGG 0.658603 4.3:+30939504 MS.gene031733:CDS
CATTGTTCTCAACATCGGCA+AGG 0.658985 4.3:+30940462 MS.gene031733:CDS
AATGAAGGCCACTTCTAATG+GGG 0.660527 4.3:+30938917 MS.gene031733:CDS
TTCCAACCGAGCAAGGAGCG+TGG 0.665669 4.3:-30941606 None:intergenic
TGGTGGACATGTTGAACCAG+GGG 0.666826 4.3:-30938892 None:intergenic
CATCACCACACCAATAGTGG+TGG 0.670110 4.3:+30941069 MS.gene031733:CDS
ATAACTCTAGGTTGTCTAAG+CGG 0.670281 4.3:-30939041 None:intergenic
GTGGTGGACATGTTGAACCA+GGG 0.677255 4.3:-30938893 None:intergenic
AAGACCTTCAGCCATAACAA+TGG 0.677825 4.3:+30941297 MS.gene031733:CDS
AATGGCTAAACGTTGCCCTG+AGG 0.683714 4.3:+30940042 MS.gene031733:CDS
GACCCAAAGAGAAACAAGAG+GGG 0.714136 4.3:-30939957 None:intergenic
TGTGGCGCGAAGAACCAGTG+AGG 0.782808 4.3:-30939723 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTAAAAATAATAATAT+AGG - chr4.3:30939168-30939187 None:intergenic 0.0%
!! TATTTAAAAATAATAATATA+GGG - chr4.3:30939167-30939186 None:intergenic 0.0%
!!! TTTTTTATAATTATTTTAAA+TGG + chr4.3:30939135-30939154 MS.gene031733:intron 0.0%
!! TATAGATAATATGCATTATA+CGG + chr4.3:30942297-30942316 MS.gene031733:intron 15.0%
!!! TATTATTTCCATATACTTTT+GGG + chr4.3:30940680-30940699 MS.gene031733:intron 15.0%
!!! TTATTATTTCCATATACTTT+TGG + chr4.3:30940679-30940698 MS.gene031733:intron 15.0%
!! AATGAAATAAAAGCAATGAA+AGG - chr4.3:30939113-30939132 None:intergenic 20.0%
!! ACATATCTGTACAATTAATA+AGG - chr4.3:30938979-30938998 None:intergenic 20.0%
!! ATTAATTGTACAGATATGTT+TGG + chr4.3:30938980-30938999 MS.gene031733:CDS 20.0%
!! CATATCTGTACAATTAATAA+GGG - chr4.3:30938978-30938997 None:intergenic 20.0%
!! TGATTCTATTAGATTGATTA+AGG - chr4.3:30941212-30941231 None:intergenic 20.0%
!! TTATGGTAAAAATAACAAAG+AGG - chr4.3:30940006-30940025 None:intergenic 20.0%
!!! ACACAAAATTAATTTGAGTT+TGG + chr4.3:30939421-30939440 MS.gene031733:intron 20.0%
!!! AGTTTTTTAACTGATACTAT+TGG + chr4.3:30940121-30940140 MS.gene031733:CDS 20.0%
!!! ATTAACATCTTTGTTTATTG+TGG - chr4.3:30939744-30939763 None:intergenic 20.0%
!!! CATTTTTACTTTCAATAGTT+TGG + chr4.3:30942477-30942496 MS.gene031733:intron 20.0%
! AAAATGACATCTTAATGAGA+TGG - chr4.3:30942463-30942482 None:intergenic 25.0%
! AATCTAATAGAATCATCAAG+AGG + chr4.3:30941217-30941236 MS.gene031733:CDS 25.0%
! AATCTATAGTTTATGAGGAA+AGG + chr4.3:30941962-30941981 MS.gene031733:CDS 25.0%
! AGCTTAATTAAGCACTTATA+GGG + chr4.3:30940499-30940518 MS.gene031733:intron 25.0%
! ATTTGCAATATGTGTTGTAA+TGG + chr4.3:30941030-30941049 MS.gene031733:CDS 25.0%
! CTAGAAATAAGAAGAATAGA+AGG - chr4.3:30939601-30939620 None:intergenic 25.0%
! TAAAACAGATATTCATCCAT+TGG + chr4.3:30942185-30942204 MS.gene031733:intron 25.0%
! TCTATTAGATTGATTAAGGT+AGG - chr4.3:30941208-30941227 None:intergenic 25.0%
! TGAAGAATCTATAGTTTATG+AGG + chr4.3:30941957-30941976 MS.gene031733:CDS 25.0%
!! AATGGATGAATATCTGTTTT+AGG - chr4.3:30942186-30942205 None:intergenic 25.0%
!! TTTAAAATTTATAACTCCGC+CGG + chr4.3:30941783-30941802 MS.gene031733:CDS 25.0%
!!! AAAGTAATAAAAGTGTCAGA+TGG + chr4.3:30941862-30941881 MS.gene031733:CDS 25.0%
!!! ATGTTGTTTTTTGTTCCAAA+AGG - chr4.3:30941350-30941369 None:intergenic 25.0%
!!! CTATTCTTCTTATTTCTAGT+AGG + chr4.3:30939602-30939621 MS.gene031733:CDS 25.0%
!!! TGAATTGTTTTATGCATGTA+TGG - chr4.3:30941124-30941143 None:intergenic 25.0%
!!! TGTTGTTTTTTGTTCCAAAA+GGG - chr4.3:30941349-30941368 None:intergenic 25.0%
AAAGTTGAAACAGTATCAAC+TGG + chr4.3:30942589-30942608 MS.gene031733:intron 30.0%
AAGATATTGTAACTGTGTTG+AGG + chr4.3:30942001-30942020 MS.gene031733:CDS 30.0%
AATGTTGTTGTAGCTTTCTT+CGG + chr4.3:30941688-30941707 MS.gene031733:CDS 30.0%
AATTGTACAGATATGTTTGG+TGG + chr4.3:30938983-30939002 MS.gene031733:CDS 30.0%
ACTAATGTAGCTACTATTAG+TGG + chr4.3:30940286-30940305 MS.gene031733:CDS 30.0%
AGATATTGTAACTGTGTTGA+GGG + chr4.3:30942002-30942021 MS.gene031733:CDS 30.0%
AGCAAGCAGTAAATATAACA+AGG - chr4.3:30940336-30940355 None:intergenic 30.0%
AGCTGTTATACCAATCAATT+TGG - chr4.3:30941830-30941849 None:intergenic 30.0%
ATGATAGTTACATTAAGGAG+TGG + chr4.3:30942628-30942647 MS.gene031733:intron 30.0%
ATTTCAACATGAAAATCCCA+TGG + chr4.3:30938941-30938960 MS.gene031733:CDS 30.0%
ATTTGCCCTTAAAATTGCAA+GGG + chr4.3:30939199-30939218 MS.gene031733:intron 30.0%
CAGCTTAATTAAGCACTTAT+AGG + chr4.3:30940498-30940517 MS.gene031733:intron 30.0%
CTTGAAATGCAATTACCATT+TGG - chr4.3:30941384-30941403 None:intergenic 30.0%
GCAACATGATAGTTACATTA+AGG + chr4.3:30942623-30942642 MS.gene031733:intron 30.0%
GTAATTGCATTTCAAGCTTA+TGG + chr4.3:30941388-30941407 MS.gene031733:CDS 30.0%
GTTATATTTACTGCTTGCTT+TGG + chr4.3:30940337-30940356 MS.gene031733:CDS 30.0%
GTTCATAAGGCAAGAAATAA+TGG + chr4.3:30941319-30941338 MS.gene031733:CDS 30.0%
TATAGCAAGTAAAGGCTTTA+TGG - chr4.3:30940023-30940042 None:intergenic 30.0%
TATAGTTTATGAGGAAAGGT+TGG + chr4.3:30941966-30941985 MS.gene031733:CDS 30.0%
TATTCATGCGTTTAGTACAT+TGG - chr4.3:30940878-30940897 None:intergenic 30.0%
TATTTGCCCTTAAAATTGCA+AGG + chr4.3:30939198-30939217 MS.gene031733:intron 30.0%
TGATAGTTACATTAAGGAGT+GGG + chr4.3:30942629-30942648 MS.gene031733:intron 30.0%
TTAAAATTTATAACTCCGCC+GGG + chr4.3:30941784-30941803 MS.gene031733:CDS 30.0%
TTAGCCATTATAGCAAGTAA+AGG - chr4.3:30940031-30940050 None:intergenic 30.0%
TTGGCTAATTACAAATGTTG+AGG + chr4.3:30942496-30942515 MS.gene031733:intron 30.0%
! AGAACTGTTAAGCTTCTTTT+AGG - chr4.3:30939557-30939576 None:intergenic 30.0%
! AGATATTCATCCATTGGTTA+TGG + chr4.3:30942191-30942210 MS.gene031733:intron 30.0%
! GAACTGTTAAGCTTCTTTTA+GGG - chr4.3:30939556-30939575 None:intergenic 30.0%
! GAATAAGAGCAATCTGATTT+TGG + chr4.3:30942029-30942048 MS.gene031733:CDS 30.0%
! TCTTCTTGTTTATCTTCATC+TGG - chr4.3:30941895-30941914 None:intergenic 30.0%
!! AAAGCCTTTACTTGCTATAA+TGG + chr4.3:30940024-30940043 MS.gene031733:CDS 30.0%
!! AACAAGAAGATGATCGATTT+TGG + chr4.3:30941905-30941924 MS.gene031733:CDS 30.0%
!!! ATCACTTTGACACTAGTTTT+GGG + chr4.3:30940094-30940113 MS.gene031733:CDS 30.0%
AAAACAGTGAGTGAAATTCC+AGG - chr4.3:30941766-30941785 None:intergenic 35.0%
AATATGTGTTGTAATGGCAC+TGG + chr4.3:30941036-30941055 MS.gene031733:CDS 35.0%
AATGGTCATTGTATCGAGAT+TGG + chr4.3:30940775-30940794 MS.gene031733:intron 35.0%
ACATGACAACAAAGACCAAA+TGG + chr4.3:30941366-30941385 MS.gene031733:CDS 35.0%
AGAGATGAAATTGCTGTCAT+AGG - chr4.3:30941439-30941458 None:intergenic 35.0%
AGCCATAACAATGGTTCATA+AGG + chr4.3:30941306-30941325 MS.gene031733:CDS 35.0%
ATAACTCTAGGTTGTCTAAG+CGG - chr4.3:30939044-30939063 None:intergenic 35.0%
ATCACATTCCCATTTGTACT+TGG + chr4.3:30939692-30939711 MS.gene031733:CDS 35.0%
ATGACTTCTGCAATAACTCT+AGG - chr4.3:30939056-30939075 None:intergenic 35.0%
CAAATGTAAATCTCCTTCAC+AGG - chr4.3:30940073-30940092 None:intergenic 35.0%
CATATATGCAATGCACTTGA+TGG + chr4.3:30941264-30941283 MS.gene031733:CDS 35.0%
CCACTAGACACAAAATAAAG+TGG - chr4.3:30940259-30940278 None:intergenic 35.0%
GATGGAACTATGGAATCATT+AGG + chr4.3:30941547-30941566 MS.gene031733:CDS 35.0%
GATTAAGGTAGGAATGTTGT+AGG - chr4.3:30941197-30941216 None:intergenic 35.0%
GCTGTTGAAAAACAAATGCA+GGG + chr4.3:30939470-30939489 MS.gene031733:intron 35.0%
GCTTAGCAACATTAGAGAAA+TGG + chr4.3:30942527-30942546 MS.gene031733:intron 35.0%
GGATTTCTCATGAATACCAA+GGG + chr4.3:30940427-30940446 MS.gene031733:CDS 35.0%
GGTATTCATGAGAAATCCAA+GGG - chr4.3:30940425-30940444 None:intergenic 35.0%
TAAAACCAACACCACAAAGT+AGG - chr4.3:30939982-30940001 None:intergenic 35.0%
TAATGCAGTCAGCACTTTAA+TGG + chr4.3:30940757-30940776 MS.gene031733:intron 35.0%
TATATAGTGACACGAGATAC+TGG + chr4.3:30940596-30940615 MS.gene031733:intron 35.0%
TCAGAAGGAATTCATAGACA+TGG - chr4.3:30940984-30941003 None:intergenic 35.0%
TCGAGTATCATTCTCGAATT+TGG - chr4.3:30940925-30940944 None:intergenic 35.0%
TGAGATTAATCACTCACACT+TGG - chr4.3:30942413-30942432 None:intergenic 35.0%
TGCCTTATGAACCATTGTTA+TGG - chr4.3:30941311-30941330 None:intergenic 35.0%
TGCTGTTGAAAAACAAATGC+AGG + chr4.3:30939469-30939488 MS.gene031733:intron 35.0%
TGGATTTCTCATGAATACCA+AGG + chr4.3:30940426-30940445 MS.gene031733:CDS 35.0%
TGGTATTCATGAGAAATCCA+AGG - chr4.3:30940426-30940445 None:intergenic 35.0%
TGTAAGAAACATCACTTGCA+TGG - chr4.3:30941669-30941688 None:intergenic 35.0%
TGTACATGTCAGAAGTTGTA+AGG + chr4.3:30942349-30942368 MS.gene031733:intron 35.0%
! ACCAACCCTTGCAATTTTAA+GGG - chr4.3:30939207-30939226 None:intergenic 35.0%
! ACTCGTTGGTGTTTATGAAA+TGG - chr4.3:30941526-30941545 None:intergenic 35.0%
! GAATTCCTTCTGAAAAGTGT+AGG + chr4.3:30940991-30941010 MS.gene031733:intron 35.0%
! TATTAGTGGAGCACTTTCTT+GGG + chr4.3:30940300-30940319 MS.gene031733:CDS 35.0%
! TATTCATCCATTGGTTATGG+AGG + chr4.3:30942194-30942213 MS.gene031733:intron 35.0%
! TGTCTAGTCCCAAAAGTATA+TGG - chr4.3:30940691-30940710 None:intergenic 35.0%
!! CATCACTTTGACACTAGTTT+TGG + chr4.3:30940093-30940112 MS.gene031733:CDS 35.0%
!! CGATTTTGGTCAGAGTTTTT+AGG + chr4.3:30941919-30941938 MS.gene031733:CDS 35.0%
!!! CCACTTTATTTTGTGTCTAG+TGG + chr4.3:30940256-30940275 MS.gene031733:CDS 35.0%
!!! CTGTTTTAGGATCATATTGC+TGG - chr4.3:30942173-30942192 None:intergenic 35.0%
!!! TGTTTTGACTGAGAAGATTG+AGG + chr4.3:30940210-30940229 MS.gene031733:CDS 35.0%
AAACGCATGAATACGTGGAA+TGG + chr4.3:30940885-30940904 MS.gene031733:intron 40.0%
AAACTTCTCACAACCGATGT+TGG + chr4.3:30939845-30939864 MS.gene031733:CDS 40.0%
AAGACCTTCAGCCATAACAA+TGG + chr4.3:30941297-30941316 MS.gene031733:CDS 40.0%
AATGAAGGCCACTTCTAATG+GGG + chr4.3:30938917-30938936 MS.gene031733:CDS 40.0%
AGCTACAAAAGCTCCAAAGA+GGG - chr4.3:30940155-30940174 None:intergenic 40.0%
AGGGTCAAAGCTTCTCTAAA+AGG - chr4.3:30940406-30940425 None:intergenic 40.0%
ATGAGAATAGCAGAACATCC+TGG + chr4.3:30941745-30941764 MS.gene031733:CDS 40.0%
ATGCAGGGAGGAATATTACT+AGG + chr4.3:30939485-30939504 MS.gene031733:intron 40.0%
ATTGGTATTCATGCCCTCTT+TGG + chr4.3:30940139-30940158 MS.gene031733:CDS 40.0%
CAATCACTTCCATCTAGCAA+AGG - chr4.3:30942078-30942097 None:intergenic 40.0%
CAATGAAGGCCACTTCTAAT+GGG + chr4.3:30938916-30938935 MS.gene031733:CDS 40.0%
CACTCATTTCGCGTAATGAA+TGG + chr4.3:30941571-30941590 MS.gene031733:CDS 40.0%
CATTAAGGAGTGGGATTGAA+TGG + chr4.3:30942638-30942657 MS.gene031733:intron 40.0%
CTTGATACACTAGCCAACAT+TGG + chr4.3:30939575-30939594 MS.gene031733:CDS 40.0%
GAAGAATAGAAGGCCAATGT+TGG - chr4.3:30939591-30939610 None:intergenic 40.0%
GAGCTCATTGTTCTCAACAT+CGG + chr4.3:30940457-30940476 MS.gene031733:CDS 40.0%
GATCTTTGCCAATGTTGAGT+TGG - chr4.3:30940526-30940545 None:intergenic 40.0%
GTACATGGTGTATCAGTACA+TGG + chr4.3:30942249-30942268 MS.gene031733:intron 40.0%
GTACTAAACGCATGAATACG+TGG + chr4.3:30940880-30940899 MS.gene031733:intron 40.0%
GTTGAAAAACAAATGCAGGG+AGG + chr4.3:30939473-30939492 MS.gene031733:intron 40.0%
GTTGAAACAGTATCAACTGG+TGG + chr4.3:30942592-30942611 MS.gene031733:intron 40.0%
TATAGGGTCCAACTCAACAT+TGG + chr4.3:30940515-30940534 MS.gene031733:intron 40.0%
TCAGAAACCTCCATAACCAA+TGG - chr4.3:30942204-30942223 None:intergenic 40.0%
TCAGTCAAAACACCAGCAAA+AGG - chr4.3:30940202-30940221 None:intergenic 40.0%
TCATGCGTTTAGTACATTGG+TGG - chr4.3:30940875-30940894 None:intergenic 40.0%
TCTCATGAATACCAAGGGAT+TGG + chr4.3:30940432-30940451 MS.gene031733:CDS 40.0%
TGAACCATTGTTATGGCTGA+AGG - chr4.3:30941304-30941323 None:intergenic 40.0%
TGCCAATGCCAAGTACAAAT+GGG - chr4.3:30939703-30939722 None:intergenic 40.0%
TTATACACTGCCACCACTAT+TGG - chr4.3:30941082-30941101 None:intergenic 40.0%
TTCCCATTTGTACTTGGCAT+TGG + chr4.3:30939698-30939717 MS.gene031733:CDS 40.0%
TTGAAATGCCCCATTAGAAG+TGG - chr4.3:30938928-30938947 None:intergenic 40.0%
TTGTAACTGTGTTGAGGGAA+AGG + chr4.3:30942007-30942026 MS.gene031733:CDS 40.0%
TTTCATCACCACACCAATAG+TGG + chr4.3:30941066-30941085 MS.gene031733:CDS 40.0%
! AAGGGAAGTGCATAATCCAT+GGG - chr4.3:30938960-30938979 None:intergenic 40.0%
! ATTAGTGGAGCACTTTCTTG+GGG + chr4.3:30940301-30940320 MS.gene031733:CDS 40.0%
! CAGTACCTACACTTTTCAGA+AGG - chr4.3:30940999-30941018 None:intergenic 40.0%
! CATAAACACCAACGAGTTGA+TGG + chr4.3:30941529-30941548 MS.gene031733:CDS 40.0%
! CCAAATGCCAATGTTTTACC+CGG - chr4.3:30941805-30941824 None:intergenic 40.0%
! CTATTAGTGGAGCACTTTCT+TGG + chr4.3:30940299-30940318 MS.gene031733:CDS 40.0%
! GAAATAATGGCATGCCCTTT+TGG + chr4.3:30941332-30941351 MS.gene031733:CDS 40.0%
! GACCAACCCTTGCAATTTTA+AGG - chr4.3:30939208-30939227 None:intergenic 40.0%
! TAAGGGAAGTGCATAATCCA+TGG - chr4.3:30938961-30938980 None:intergenic 40.0%
! TGAGAAGTTTGAGTTCAGCT+AGG - chr4.3:30939835-30939854 None:intergenic 40.0%
! TGTACTGATACACCATGTAC+TGG - chr4.3:30942249-30942268 None:intergenic 40.0%
! TTCCCCTCTTGTTTCTCTTT+GGG + chr4.3:30939955-30939974 MS.gene031733:CDS 40.0%
! TTTAGAGAAGCTTTGACCCT+TGG + chr4.3:30940406-30940425 MS.gene031733:CDS 40.0%
!! AAGAGCAATCTGATTTTGGC+AGG + chr4.3:30942033-30942052 MS.gene031733:CDS 40.0%
!! GCATTTGGTGCCAAATTGAT+TGG + chr4.3:30941817-30941836 MS.gene031733:CDS 40.0%
!! GCTTGCTTTGGAAAAGTTGT+TGG + chr4.3:30940349-30940368 MS.gene031733:CDS 40.0%
!! TGCGTGTGATTGTTGATAAC+TGG - chr4.3:30940736-30940755 None:intergenic 40.0%
!!! GTTGGAATTTTGGTTGATCG+CGG + chr4.3:30941622-30941641 MS.gene031733:CDS 40.0%
AACATGTCCACCACCAATGA+AGG + chr4.3:30938902-30938921 MS.gene031733:CDS 45.0%
AACGCCCATGAAAACATGGA+AGG - chr4.3:30939774-30939793 None:intergenic 45.0%
AAGATCCAGACACAGAGCTT+AGG + chr4.3:30941149-30941168 MS.gene031733:CDS 45.0%
ACCAAAATTCCAACCGAGCA+AGG - chr4.3:30941616-30941635 None:intergenic 45.0%
AGGACCCAAAGAGAAACAAG+AGG - chr4.3:30939962-30939981 None:intergenic 45.0%
AGTATCCTAAGCTCTGTGTC+TGG - chr4.3:30941157-30941176 None:intergenic 45.0%
ATGAAAACATGGAAGGCTGC+TGG - chr4.3:30939767-30939786 None:intergenic 45.0%
ATGCGTTCGATACGCAAAAC+AGG + chr4.3:30939638-30939657 MS.gene031733:CDS 45.0%
ATGGGAATGTGATTCCAGCA+AGG - chr4.3:30939685-30939704 None:intergenic 45.0%
CAGCTACAAAAGCTCCAAAG+AGG - chr4.3:30940156-30940175 None:intergenic 45.0%
CATGAATACCAAGGGATTGG+TGG + chr4.3:30940435-30940454 MS.gene031733:CDS 45.0%
CATTGCCATACGACCAACAT+CGG - chr4.3:30939861-30939880 None:intergenic 45.0%
CATTGTTCTCAACATCGGCA+AGG + chr4.3:30940462-30940481 MS.gene031733:CDS 45.0%
CCAATGAAGGCCACTTCTAA+TGG + chr4.3:30938915-30938934 MS.gene031733:CDS 45.0%
CCATAGTTCCATCAACTCGT+TGG - chr4.3:30941540-30941559 None:intergenic 45.0%
CCATTAGAAGTGGCCTTCAT+TGG - chr4.3:30938918-30938937 None:intergenic 45.0%
CCGGGTAAAACATTGGCATT+TGG + chr4.3:30941802-30941821 MS.gene031733:CDS 45.0%
CTGTCAATGATGTTGCTGCT+TGG + chr4.3:30939891-30939910 MS.gene031733:CDS 45.0%
GAAGCCAAATAGCTTCCAAC+AGG - chr4.3:30942114-30942133 None:intergenic 45.0%
GACCCAAAGAGAAACAAGAG+GGG - chr4.3:30939960-30939979 None:intergenic 45.0%
GCCCTTAAAATTGCAAGGGT+TGG + chr4.3:30939203-30939222 MS.gene031733:intron 45.0%
GCTCTTGCAATAGCTCTTTC+TGG + chr4.3:30939923-30939942 MS.gene031733:CDS 45.0%
GGACCCAAAGAGAAACAAGA+GGG - chr4.3:30939961-30939980 None:intergenic 45.0%
GTGCCAATGCCAAGTACAAA+TGG - chr4.3:30939704-30939723 None:intergenic 45.0%
GTGTATAAACCAGCTAGGAG+AGG + chr4.3:30941094-30941113 MS.gene031733:CDS 45.0%
GTTGTTGTAGCTTTCTTCGG+AGG + chr4.3:30941691-30941710 MS.gene031733:CDS 45.0%
TACTAGGACCCTCAGCAATT+GGG + chr4.3:30939501-30939520 MS.gene031733:CDS 45.0%
TCTCTTTGGGTCCTACTTTG+TGG + chr4.3:30939968-30939987 MS.gene031733:CDS 45.0%
TGGCAGTGTATAAACCAGCT+AGG + chr4.3:30941089-30941108 MS.gene031733:CDS 45.0%
TGGGAATGTGATTCCAGCAA+GGG - chr4.3:30939684-30939703 None:intergenic 45.0%
TGGTCCTGTTGGAAGCTATT+TGG + chr4.3:30942107-30942126 MS.gene031733:CDS 45.0%
TTACTAGGACCCTCAGCAAT+TGG + chr4.3:30939500-30939519 MS.gene031733:CDS 45.0%
TTAGAAGTGGCCTTCATTGG+TGG - chr4.3:30938915-30938934 None:intergenic 45.0%
TTGGGACTAGACAAGAGAGA+GGG + chr4.3:30940698-30940717 MS.gene031733:intron 45.0%
! AATGCCAATGTTTTACCCGG+CGG - chr4.3:30941802-30941821 None:intergenic 45.0%
! AGAAACAAGAGGGGAAGTGT+TGG - chr4.3:30939951-30939970 None:intergenic 45.0%
! AGCAGCCTTCCATGTTTTCA+TGG + chr4.3:30939766-30939785 MS.gene031733:CDS 45.0%
! AGCTGGCATTGTTGTTCCTA+AGG + chr4.3:30940171-30940190 MS.gene031733:CDS 45.0%
! CTTCCCCTCTTGTTTCTCTT+TGG + chr4.3:30939954-30939973 MS.gene031733:CDS 45.0%
! GAATCATCGCAACTCGCTTT+TGG - chr4.3:30941498-30941517 None:intergenic 45.0%
! GCAGCCTTCCATGTTTTCAT+GGG + chr4.3:30939767-30939786 MS.gene031733:CDS 45.0%
! GGCATTGTTGTTCCTAAGGA+TGG + chr4.3:30940175-30940194 MS.gene031733:CDS 45.0%
! TAGGAATGTTGTAGGTGCTG+TGG - chr4.3:30941189-30941208 None:intergenic 45.0%
! TCCTTGCTCGGTTGGAATTT+TGG + chr4.3:30941612-30941631 MS.gene031733:CDS 45.0%
! TTTGGGACTAGACAAGAGAG+AGG + chr4.3:30940697-30940716 MS.gene031733:intron 45.0%
!! CCAACGAGTTGATGGAACTA+TGG + chr4.3:30941537-30941556 MS.gene031733:CDS 45.0%
!!! CTCTTTGGAGCTTTTGTAGC+TGG + chr4.3:30940154-30940173 MS.gene031733:CDS 45.0%
AACTCCGCCGGGTAAAACAT+TGG + chr4.3:30941795-30941814 MS.gene031733:CDS 50.0%
AATGAGCTCCACCAATCCCT+TGG - chr4.3:30940446-30940465 None:intergenic 50.0%
AATGGCTAAACGTTGCCCTG+AGG + chr4.3:30940042-30940061 MS.gene031733:CDS 50.0%
ATGCCTGACACACCAGTACA+TGG + chr4.3:30942234-30942253 MS.gene031733:intron 50.0%
ATGGCTAAACGTTGCCCTGA+GGG + chr4.3:30940043-30940062 MS.gene031733:CDS 50.0%
CACAACCGATGTTGGTCGTA+TGG + chr4.3:30939853-30939872 MS.gene031733:CDS 50.0%
CATCACCACACCAATAGTGG+TGG + chr4.3:30941069-30941088 MS.gene031733:CDS 50.0%
CCAGCAAAAGGACCATCCTT+AGG - chr4.3:30940190-30940209 None:intergenic 50.0%
CCAGTAGCACCTTTGCTAGA+TGG + chr4.3:30942066-30942085 MS.gene031733:CDS 50.0%
CTGTTCCTCCCAATTGCTGA+GGG - chr4.3:30939512-30939531 None:intergenic 50.0%
GAGCAACGCCCATGAAAACA+TGG - chr4.3:30939778-30939797 None:intergenic 50.0%
GATTCGAGCAAGGACAGGAA+AGG - chr4.3:30939813-30939832 None:intergenic 50.0%
GGAAGTGATTGTGCTGAGCT+TGG + chr4.3:30942087-30942106 MS.gene031733:CDS 50.0%
GTTCAGCTAGGATTCGAGCA+AGG - chr4.3:30939823-30939842 None:intergenic 50.0%
TATGGTGATCCTCTCCTAGC+TGG - chr4.3:30941106-30941125 None:intergenic 50.0%
TCAAGAGGAACACGAAAGCG+AGG + chr4.3:30941232-30941251 MS.gene031733:CDS 50.0%
TCCAGCAAGGGCTATGCATA+AGG - chr4.3:30939672-30939691 None:intergenic 50.0%
TGGGACTAGACAAGAGAGAG+GGG + chr4.3:30940699-30940718 MS.gene031733:intron 50.0%
TGGGTCCTACTTTGTGGTGT+TGG + chr4.3:30939974-30939993 MS.gene031733:CDS 50.0%
! ACACCATGTACTGGTGTGTC+AGG - chr4.3:30942240-30942259 None:intergenic 50.0%
! CCTAAGGATGGTCCTTTTGC+TGG + chr4.3:30940187-30940206 MS.gene031733:CDS 50.0%
! TGGTGGACATGTTGAACCAG+GGG - chr4.3:30938895-30938914 None:intergenic 50.0%
!! AGACAAGAGAGAGGGGCATT+TGG + chr4.3:30940706-30940725 MS.gene031733:intron 50.0%
!! CCATCTAGCAAAGGTGCTAC+TGG - chr4.3:30942069-30942088 None:intergenic 50.0%
!! GTGGTGGACATGTTGAACCA+GGG - chr4.3:30938896-30938915 None:intergenic 50.0%
!! TCTAGCAAAGGTGCTACTGG+AGG - chr4.3:30942066-30942085 None:intergenic 50.0%
!!! ATTTTGGTTGATCGCGGCCT+TGG + chr4.3:30941628-30941647 MS.gene031733:CDS 50.0%
ACTTAGCCACGCTCCTTGCT+CGG + chr4.3:30941600-30941619 MS.gene031733:CDS 55.0%
CAACATCGGCAAGGATCGCA+AGG + chr4.3:30940471-30940490 MS.gene031733:CDS 55.0%
CACTGCCACCACTATTGGTG+TGG - chr4.3:30941077-30941096 None:intergenic 55.0%
CCTCAGCAATTGGGAGGAAC+AGG + chr4.3:30939510-30939529 MS.gene031733:CDS 55.0%
CCTGTTCCTCCCAATTGCTG+AGG - chr4.3:30939513-30939532 None:intergenic 55.0%
CCTTCACAGGTTCACCCTCA+GGG - chr4.3:30940060-30940079 None:intergenic 55.0%
GAAGTGGCCTTCATTGGTGG+TGG - chr4.3:30938912-30938931 None:intergenic 55.0%
GCCTTATGCATAGCCCTTGC+TGG + chr4.3:30939668-30939687 MS.gene031733:CDS 55.0%
GCTAGGATTCGAGCAAGGAC+AGG - chr4.3:30939818-30939837 None:intergenic 55.0%
TAGGACCCTCAGCAATTGGG+AGG + chr4.3:30939504-30939523 MS.gene031733:CDS 55.0%
TCCTTCACAGGTTCACCCTC+AGG - chr4.3:30940061-30940080 None:intergenic 55.0%
TGTGCTGAGCTTGGTCCTGT+TGG + chr4.3:30942096-30942115 MS.gene031733:CDS 55.0%
! GATCGCGAAGCACTTGCGTA+CGG + chr4.3:30941721-30941740 MS.gene031733:CDS 55.0%
! GGACTTGAGTTGTCCCTCCA+AGG - chr4.3:30941648-30941667 None:intergenic 55.0%
!! ACTTGGCATTGGCACCTCAC+TGG + chr4.3:30939709-30939728 MS.gene031733:CDS 55.0%
!! GGTGGTGGACATGTTGAACC+AGG - chr4.3:30938897-30938916 None:intergenic 55.0%
CCCTGAGGGTGAACCTGTGA+AGG + chr4.3:30940057-30940076 MS.gene031733:CDS 60.0%
TGGTTGATCGCGGCCTTGGA+GGG + chr4.3:30941632-30941651 MS.gene031733:CDS 60.0%
TGTGGCGCGAAGAACCAGTG+AGG - chr4.3:30939726-30939745 None:intergenic 60.0%
TTCCAACCGAGCAAGGAGCG+TGG - chr4.3:30941609-30941628 None:intergenic 60.0%
TTGGTTGATCGCGGCCTTGG+AGG + chr4.3:30941631-30941650 MS.gene031733:CDS 60.0%
AGCCACGCTCCTTGCTCGGT+TGG + chr4.3:30941604-30941623 MS.gene031733:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 30938885 30942831 30938885 ID=MS.gene031733
chr4.3 mRNA 30938885 30942831 30938885 ID=MS.gene031733.t1;Parent=MS.gene031733
chr4.3 exon 30938885 30939076 30938885 ID=MS.gene031733.t1.exon1;Parent=MS.gene031733.t1
chr4.3 CDS 30938885 30939076 30938885 ID=cds.MS.gene031733.t1;Parent=MS.gene031733.t1
chr4.3 exon 30939491 30940492 30939491 ID=MS.gene031733.t1.exon2;Parent=MS.gene031733.t1
chr4.3 CDS 30939491 30940492 30939491 ID=cds.MS.gene031733.t1;Parent=MS.gene031733.t1
chr4.3 exon 30941013 30942158 30941013 ID=MS.gene031733.t1.exon3;Parent=MS.gene031733.t1
chr4.3 CDS 30941013 30942158 30941013 ID=cds.MS.gene031733.t1;Parent=MS.gene031733.t1
chr4.3 exon 30942796 30942831 30942796 ID=MS.gene031733.t1.exon4;Parent=MS.gene031733.t1
chr4.3 CDS 30942796 30942831 30942796 ID=cds.MS.gene031733.t1;Parent=MS.gene031733.t1
Gene Sequence

>MS.gene031733

ATGGCTACCCCTGGTTCAACATGTCCACCACCAATGAAGGCCACTTCTAATGGGGCATTTCAACATGAAAATCCCATGGATTATGCACTTCCCTTATTAATTGTACAGATATGTTTGGTGGTCGCTTTTACAAGATTCATTGCATTTCTTTGCAAGCCGCTTAGACAACCTAGAGTTATTGCAGAAGTCATTGTAAGCACTTTTTTTCTTCTTAATATTATAGAACCTTTCATTGCTTTTATTTCATTTCTTTTTTATAATTATTTTAAATGGATACATCCCTATATTATTATTTTTAAATATGTTTTTTTTCTATTTGCCCTTAAAATTGCAAGGGTTGGTCTTTTTAGTTCGTGAAATTTGTGTATTGACAAAAACATCTTTAAATTTGTAAGAACATCAATCAATTTAGTCATTCTATCAAGTTCTTCTGTTACGTGTGACACATTACAATATTTTGTTATCATGTGTCAAACTCAGAATCTCTAAATCATGATATTGACTATTTTGTTTGAGTTCTTCTTATTCAAATTCATACACAAAATTAATTTGAGTTTGGATTTGCATGTCTCACATAATGTTTGTGCTGTTGAAAAACAAATGCAGGGAGGAATATTACTAGGACCCTCAGCAATTGGGAGGAACAGGAAATTTCTAGATACATTCTTCCCTAAAAGAAGCTTAACAGTTCTTGATACACTAGCCAACATTGGCCTTCTATTCTTCTTATTTCTAGTAGGTCTTGAACTCGACATGCGTTCGATACGCAAAACAGGTTCAAAAGCCTTATGCATAGCCCTTGCTGGAATCACATTCCCATTTGTACTTGGCATTGGCACCTCACTGGTTCTTCGCGCCACAATAAACAAAGATGTTAATCCAGCAGCCTTCCATGTTTTCATGGGCGTTGCTCTTTCGATCACAGCCTTTCCTGTCCTTGCTCGAATCCTAGCTGAACTCAAACTTCTCACAACCGATGTTGGTCGTATGGCAATGTCAGCTGCTGCTGTCAATGATGTTGCTGCTTGGATACTTCTTGCTCTTGCAATAGCTCTTTCTGGCGCCAACACTTCCCCTCTTGTTTCTCTTTGGGTCCTACTTTGTGGTGTTGGTTTTATCCTCTTTGTTATTTTTACCATAAAGCCTTTACTTGCTATAATGGCTAAACGTTGCCCTGAGGGTGAACCTGTGAAGGAGATTTACATTTGCATCACTTTGACACTAGTTTTGGGTTGTAGTTTTTTAACTGATACTATTGGTATTCATGCCCTCTTTGGAGCTTTTGTAGCTGGCATTGTTGTTCCTAAGGATGGTCCTTTTGCTGGTGTTTTGACTGAGAAGATTGAGGATCTTGTAATGAGTCTTTTGTTGCCACTTTATTTTGTGTCTAGTGGATTGAAGACTAATGTAGCTACTATTAGTGGAGCACTTTCTTGGGGATTATTAATCCTTGTTATATTTACTGCTTGCTTTGGAAAAGTTGTTGGCACATTAGTTGTGTCATTGTTGTGTAAGATTCCTTTTAGAGAAGCTTTGACCCTTGGATTTCTCATGAATACCAAGGGATTGGTGGAGCTCATTGTTCTCAACATCGGCAAGGATCGCAAGGTAATCAGCTTAATTAAGCACTTATAGGGTCCAACTCAACATTGGCAAAGATCACAAATTAGTCTTTAAATTATATGAAATTCGTTAAATTAGTCTTTGTCGTTATATAGTGACACGAGATACTGGTAACTAAAGAGATATATTTCAAATAATTTATTGTTTAACTTAACAGATTTCATATAATATTTATTATTTCCATATACTTTTGGGACTAGACAAGAGAGAGGGGCATTTGGATTGCCAGTTATCAACAATCACACGCATTAATGCAGTCAGCACTTTAATGGTCATTGTATCGAGATTGGAAGTTCAAGATTTTGATTTTTAACATCTAAAATATTGAGCTTGAAATTTGAACAGTTCAATCTCGATACAACTGCCACCAATGTACTAAACGCATGAATACGTGGAATGGTGACTGCTAACGATCCAAATTCGAGAATGATACTCGAGAGACTATATAGATCGTTTACTAAAATAATAATAAACCATGTCTATGAATTCCTTCTGAAAAGTGTAGGTACTGAGTGATCAATCATTTGCAATATGTGTTGTAATGGCACTGGTCACAACTTTCATCACCACACCAATAGTGGTGGCAGTGTATAAACCAGCTAGGAGAGGATCACCATACATGCATAAAACAATTCAGCGCAAAGATCCAGACACAGAGCTTAGGATACTAGCTTGTTTCCACAGCACCTACAACATTCCTACCTTAATCAATCTAATAGAATCATCAAGAGGAACACGAAAGCGAGGAAAGTTATGCATATATGCAATGCACTTGATGGAACTCTCAGAAAGACCTTCAGCCATAACAATGGTTCATAAGGCAAGAAATAATGGCATGCCCTTTTGGAACAAAAAACAACATGACAACAAAGACCAAATGGTAATTGCATTTCAAGCTTATGGACATTTAAGTAGTGTCAATGTTCGTCCTATGACAGCAATTTCATCTCTCAATAACATTCATGAAGACATTTGTTCTAGTGCACACCAAAAGCGAGTTGCGATGATTCTACTTCCATTTCATAAACACCAACGAGTTGATGGAACTATGGAATCATTAGGACACTCATTTCGCGTAATGAATGGACTTGTACTTAGCCACGCTCCTTGCTCGGTTGGAATTTTGGTTGATCGCGGCCTTGGAGGGACAACTCAAGTCCATGCAAGTGATGTTTCTTACAATGTTGTTGTAGCTTTCTTCGGAGGATGTGATGATCGCGAAGCACTTGCGTACGGTATGAGAATAGCAGAACATCCTGGAATTTCACTCACTGTTTTAAAATTTATAACTCCGCCGGGTAAAACATTGGCATTTGGTGCCAAATTGATTGGTATAACAGCTAACAGAGATAGAAAAGTAATAAAAGTGTCAGATGGTAACACACCAGATGAAGATAAACAAGAAGATGATCGATTTTGGTCAGAGTTTTTAGGTGTTTGTTGTAAGAGTGAAGAATCTATAGTTTATGAGGAAAGGTTGGTTGAAAGTAAAGAAGATATTGTAACTGTGTTGAGGGAAAGGAATAAGAGCAATCTGATTTTGGCAGGTAGAATGCCTCCAGTAGCACCTTTGCTAGATGGAAGTGATTGTGCTGAGCTTGGTCCTGTTGGAAGCTATTTGGCTTCTTCTGAGTTTTCTACATTTGCATCAGTTATAGTTTTCCAGCAATATGATCCTAAAACAGATATTCATCCATTGGTTATGGAGGTTTCTGATTACTCTAATATGCCTGACACACCAGTACATGGTGTATCAGTACATGGTGTATAGAATAGATATAAATATAGATATAGATAATATGCATTATACGGTTAAGCTAATGTTGTTGTTTGCATGATTTTGTACATGTCAGAAGTTGTAAGGTAATTGTTTGAGAAATTTTTGTGTCTGAATTAGTGAATCCAAGTGTGAGTGATTAATCTCACATCGAATTATAAATAAGAGTTATGTTCCATCTCATTAAGATGTCATTTTTACTTTCAATAGTTTGGCTAATTACAAATGTTGAGGTGTTGCTTGCTTAGCAACATTAGAGAAATGGTATATGATAAACAGAAATTACATTTGTCAATGCTTGTTAAAAGTTGAAACAGTATCAACTGGTGGAAGTTTTAGCAACATGATAGTTACATTAAGGAGTGGGATTGAATGGTAAAAAGAAAACATATTTTTTTATAAAAGTAAAATTAAACACATATGCATGTAGTTTGTTATTTTTTTGTCAAAAAAAACGTATCTAGATTATTATTTTAATGAGAAACTCTTAAATATTTGTGAAACTGAAAAGATAAATGTTAACGAGTACCCTAAGTGCACTTATTAG
Protein sequence

>MS.gene031733.t1

MATPGSTCPPPMKATSNGAFQHENPMDYALPLLIVQICLVVAFTRFIAFLCKPLRQPRVIAEVIGGILLGPSAIGRNRKFLDTFFPKRSLTVLDTLANIGLLFFLFLVGLELDMRSIRKTGSKALCIALAGITFPFVLGIGTSLVLRATINKDVNPAAFHVFMGVALSITAFPVLARILAELKLLTTDVGRMAMSAAAVNDVAAWILLALAIALSGANTSPLVSLWVLLCGVGFILFVIFTIKPLLAIMAKRCPEGEPVKEIYICITLTLVLGCSFLTDTIGIHALFGAFVAGIVVPKDGPFAGVLTEKIEDLVMSLLLPLYFVSSGLKTNVATISGALSWGLLILVIFTACFGKVVGTLVVSLLCKIPFREALTLGFLMNTKGLVELIVLNIGKDRKVLSDQSFAICVVMALVTTFITTPIVVAVYKPARRGSPYMHKTIQRKDPDTELRILACFHSTYNIPTLINLIESSRGTRKRGKLCIYAMHLMELSERPSAITMVHKARNNGMPFWNKKQHDNKDQMVIAFQAYGHLSSVNVRPMTAISSLNNIHEDICSSAHQKRVAMILLPFHKHQRVDGTMESLGHSFRVMNGLVLSHAPCSVGILVDRGLGGTTQVHASDVSYNVVVAFFGGCDDREALAYGMRIAEHPGISLTVLKFITPPGKTLAFGAKLIGITANRDRKVIKVSDGNTPDEDKQEDDRFWSEFLGVCCKSEESIVYEERLVESKEDIVTVLRERNKSNLILAGRMPPVAPLLDGSDCAELGPVGSYLASSEFSTFASINVNEYPKCTY