AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68584

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68584.t1 MTR_4g123583 98.890 811 9 0 1 811 1 811 0.0 1647
MS.gene68584.t1 MTR_4g078120 78.765 810 171 1 1 809 1 810 0.0 1297
MS.gene68584.t1 MTR_5g009780 59.572 794 305 4 3 793 6 786 0.0 968
MS.gene68584.t1 MTR_5g009770 59.949 789 298 4 3 788 6 779 0.0 964
MS.gene68584.t1 MTR_5g009800 56.599 788 305 6 9 793 12 765 0.0 905
MS.gene68584.t1 MTR_5g009770 58.522 663 257 4 129 788 1 648 0.0 789
MS.gene68584.t1 MTR_8g093780 45.739 798 414 10 1 786 1 791 0.0 723
MS.gene68584.t1 MTR_3g096380 45.443 790 411 10 6 786 12 790 0.0 707
MS.gene68584.t1 MTR_7g099800 51.816 826 331 23 12 787 8 816 0.0 690
MS.gene68584.t1 MTR_2g011520 43.918 781 410 9 15 786 3 764 0.0 671
MS.gene68584.t1 MTR_7g099750 48.678 832 345 20 12 789 8 811 0.0 660
MS.gene68584.t1 MTR_7g099820 48.120 798 368 15 13 784 5 782 0.0 653
MS.gene68584.t1 MTR_7g099820 52.941 663 286 9 13 657 5 659 0.0 632
MS.gene68584.t1 MTR_3g031170 33.206 786 481 10 8 785 29 778 1.19e-139 432
MS.gene68584.t1 MTR_2g040100 33.375 797 479 17 20 786 36 810 3.04e-132 414
MS.gene68584.t1 MTR_3g037270 32.107 788 491 14 20 786 25 789 1.05e-129 407
MS.gene68584.t1 MTR_5g067690 32.050 805 504 14 3 786 10 792 8.41e-129 405
MS.gene68584.t1 MTR_1g043400 31.773 812 493 16 7 786 3 785 5.65e-128 402
MS.gene68584.t1 MTR_3g037260 31.345 788 499 14 20 786 21 787 6.22e-126 397
MS.gene68584.t1 MTR_0002s1180 31.546 802 511 13 5 786 3 786 2.99e-125 395
MS.gene68584.t1 MTR_5g074360 30.976 820 504 19 3 798 14 795 1.52e-124 394
MS.gene68584.t1 MTR_1g045870 31.566 811 481 17 7 786 3 770 1.07e-122 388
MS.gene68584.t1 MTR_3g071990 30.025 796 501 16 6 785 10 765 1.96e-122 388
MS.gene68584.t1 MTR_5g074380 31.083 785 492 16 16 785 76 826 3.12e-121 386
MS.gene68584.t1 MTR_5g074340 29.963 811 503 18 15 802 30 798 1.20e-119 381
MS.gene68584.t1 MTR_5g074320 30.821 743 451 17 65 785 10 711 9.53e-109 350
MS.gene68584.t1 MTR_3g071990 30.278 720 446 17 82 785 4 683 1.27e-107 347
MS.gene68584.t1 MTR_3g031160 28.340 801 516 17 6 788 22 782 1.50e-104 340
MS.gene68584.t1 MTR_3g071880 27.945 798 515 20 18 784 22 790 2.16e-102 335
MS.gene68584.t1 MTR_3g498715 28.554 802 503 19 5 781 32 788 9.51e-98 322
MS.gene68584.t1 MTR_6g015940 29.383 810 499 19 3 785 22 785 5.18e-96 317
MS.gene68584.t1 MTR_4g037755 28.278 778 496 19 49 798 38 781 2.79e-95 315
MS.gene68584.t1 MTR_6g053320 30.108 744 447 21 80 792 71 772 5.01e-95 314
MS.gene68584.t1 MTR_3g071870 28.714 801 506 21 16 785 33 799 1.18e-94 314
MS.gene68584.t1 MTR_8g085260 27.736 804 508 17 10 785 31 789 2.02e-90 302
MS.gene68584.t1 MTR_5g088790 27.662 817 514 16 5 785 39 814 5.74e-89 299
MS.gene68584.t1 MTR_5g088450 26.790 810 524 20 5 785 22 791 1.13e-88 298
MS.gene68584.t1 MTR_3g083850 26.814 813 515 21 10 791 27 790 1.41e-88 297
MS.gene68584.t1 MTR_5g088430 26.601 812 522 21 10 791 27 794 6.22e-88 296
MS.gene68584.t1 MTR_5g088470 27.261 807 521 24 5 785 22 788 6.36e-88 295
MS.gene68584.t1 MTR_7g053000 27.050 817 519 17 5 785 39 814 3.65e-87 294
MS.gene68584.t1 MTR_3g031030 27.898 785 500 18 39 795 28 774 1.05e-86 292
MS.gene68584.t1 MTR_7g052680 27.173 817 518 17 5 785 39 814 1.95e-86 292
MS.gene68584.t1 MTR_7g052970 27.050 817 519 17 5 785 39 814 2.18e-86 292
MS.gene68584.t1 MTR_4g029520 26.166 772 532 15 35 786 25 778 2.61e-84 285
MS.gene68584.t1 MTR_3g110700 26.726 782 533 16 24 786 15 775 3.75e-83 282
MS.gene68584.t1 MTR_5g088840 27.050 817 520 19 5 785 39 815 1.39e-82 281
MS.gene68584.t1 MTR_7g085870 26.167 814 517 20 13 785 24 794 2.56e-82 281
MS.gene68584.t1 MTR_7g052895 25.665 526 358 8 5 505 39 556 5.11e-52 194
MS.gene68584.t1 MTR_3g465680 30.534 393 265 6 17 404 44 433 6.50e-52 196
MS.gene68584.t1 MTR_3g465680 27.543 403 235 11 398 785 555 915 5.14e-35 144
MS.gene68584.t1 MTR_1g082340 82.474 97 16 1 452 547 82 178 8.35e-49 172
MS.gene68584.t1 MTR_5g061460 80.412 97 18 1 452 547 15 111 7.86e-47 164
MS.gene68584.t1 MTR_5g083560 29.697 330 190 9 466 785 1 298 2.28e-25 108
MS.gene68584.t1 MTR_1g023830 79.592 49 9 1 500 547 1 49 9.24e-21 87.8
MS.gene68584.t1 MTR_3g083860 25.243 206 117 7 2 202 19 192 1.22e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68584.t1 AT3G17630 70.647 804 216 3 1 801 1 787 0.0 1131
MS.gene68584.t1 AT3G17630 70.647 804 216 3 1 801 1 787 0.0 1131
MS.gene68584.t1 AT5G41610 62.453 799 279 7 1 789 1 788 0.0 989
MS.gene68584.t1 AT5G41610 62.453 799 279 7 1 789 1 788 0.0 989
MS.gene68584.t1 AT4G23700 60.875 800 294 9 1 786 1 795 0.0 958
MS.gene68584.t1 AT4G23700 60.875 800 294 9 1 786 1 795 0.0 958
MS.gene68584.t1 AT5G41610 61.970 731 258 6 68 789 1 720 0.0 891
MS.gene68584.t1 AT1G64170 52.905 809 329 14 4 786 10 792 0.0 797
MS.gene68584.t1 AT2G13620 47.297 814 397 10 5 806 12 805 0.0 731
MS.gene68584.t1 AT3G53720 51.345 818 347 17 12 787 8 816 0.0 691
MS.gene68584.t1 AT3G53720 50.933 750 317 17 80 787 3 743 0.0 608
MS.gene68584.t1 AT1G05580 35.451 787 468 12 19 789 40 802 1.51e-147 455
MS.gene68584.t1 AT1G05580 35.854 714 418 12 92 789 2 691 8.73e-134 416
MS.gene68584.t1 AT2G31910 33.037 787 487 14 19 788 26 789 1.29e-133 418
MS.gene68584.t1 AT2G31910 33.333 714 436 14 92 788 2 692 3.37e-119 377
MS.gene68584.t1 AT2G31910 33.333 714 436 14 92 788 2 692 3.37e-119 377
MS.gene68584.t1 AT1G06970 31.380 819 511 18 5 811 25 804 2.47e-118 377
MS.gene68584.t1 AT2G30240 31.021 793 499 17 6 785 28 785 7.31e-105 342
MS.gene68584.t1 AT5G37060 26.773 818 532 13 6 785 41 829 6.46e-91 305
MS.gene68584.t1 AT5G01690 28.247 793 512 16 8 785 17 767 8.80e-89 297
MS.gene68584.t1 AT5G58460 26.368 804 527 13 18 785 53 827 1.41e-88 299
MS.gene68584.t1 AT3G44900 27.399 792 512 19 27 785 51 812 1.07e-86 293
MS.gene68584.t1 AT5G22900 26.087 828 522 20 11 785 27 817 1.13e-84 287
MS.gene68584.t1 AT3G52080 27.934 784 508 19 32 785 28 784 1.03e-78 271
MS.gene68584.t1 AT1G79400 26.343 782 542 14 24 791 17 778 1.89e-76 264
MS.gene68584.t1 AT2G28180 27.538 788 513 18 28 791 61 814 9.03e-76 263
MS.gene68584.t1 AT1G16380 25.409 795 535 17 24 791 17 780 1.21e-72 253
MS.gene68584.t1 AT5G22910 26.422 791 502 21 21 774 37 784 2.83e-70 247
MS.gene68584.t1 AT5G01680 25.403 807 539 18 2 785 9 775 3.52e-70 247
MS.gene68584.t1 AT1G08150 26.761 781 504 22 27 779 62 802 2.27e-69 245
MS.gene68584.t1 AT3G44930 25.546 779 506 17 28 776 35 769 1.34e-65 234
MS.gene68584.t1 AT3G44910 25.548 775 499 16 28 776 35 757 1.25e-64 231
MS.gene68584.t1 AT1G08140 25.095 793 517 20 28 785 53 803 1.21e-63 229
MS.gene68584.t1 AT2G28170 25.377 796 506 23 27 785 54 798 1.87e-62 225
MS.gene68584.t1 AT3G44920 25.443 790 507 18 28 783 35 776 2.59e-54 201
MS.gene68584.t1 AT1G08135 24.938 802 495 26 27 785 57 794 2.41e-53 199
MS.gene68584.t1 AT5G51710 22.759 435 274 13 33 439 154 554 6.87e-13 72.8
MS.gene68584.t1 AT5G51710 23.188 414 268 12 33 430 154 533 9.95e-13 72.0

Find 181 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTGTTCTGTTGGAATTT+TGG 0.120950 8.2:-9453080 MS.gene68584:CDS
CATCGATACGCTGGTGTTTA+TGG 0.150896 8.2:+9453164 None:intergenic
ATTGGAATTCATGCATTATT+TGG 0.159696 8.2:-9454431 MS.gene68584:CDS
AGCAAGTGAAGTTTCCTTTA+AGG 0.186167 8.2:-9453023 MS.gene68584:CDS
ACAAAGTCTTCCTCGCTTTC+GGG 0.227411 8.2:+9453447 None:intergenic
TGTCCCTTTCCTTGTCTTCA+TGG 0.230128 8.2:-9454804 MS.gene68584:CDS
TATCACATTGACATTGGTTT+TGG 0.230633 8.2:-9454477 MS.gene68584:CDS
ATTTGCAATTTGTGTTCTTA+TGG 0.243891 8.2:-9453659 MS.gene68584:CDS
TCCTCTTGTTATGGAGGAAT+TGG 0.251015 8.2:-9452492 MS.gene68584:CDS
GAAGATTACAACAACCTTAA+AGG 0.252758 8.2:+9453009 None:intergenic
TACAAAGTCTTCCTCGCTTT+CGG 0.253530 8.2:+9453446 None:intergenic
TGTATACTGCCATCACTATT+GGG 0.269576 8.2:+9453611 None:intergenic
GCAACAATGGAATGCCCTTT+TGG 0.272307 8.2:-9453357 MS.gene68584:CDS
TCAGCTGAACTTGGACCTAT+TGG 0.290347 8.2:-9452596 MS.gene68584:CDS
CATTCATGCTCTTTGCACTA+TGG 0.290611 8.2:+9452756 None:intergenic
CTATAAGTGGAGGGGTTTCT+TGG 0.297538 8.2:-9454271 MS.gene68584:CDS
CTCCACTTCTGCATCAATCT+TGG 0.302772 8.2:-9452549 MS.gene68584:CDS
AGTGCAAGAGCTTCTCTAAA+AGG 0.305255 8.2:+9454167 None:intergenic
ATCACATTCCCATTTGTTCT+TGG 0.308008 8.2:-9454878 MS.gene68584:CDS
GAGCAAGAGTAATTTGATAT+TGG 0.309795 8.2:-9452663 MS.gene68584:CDS
CCTGGAATGACATTGTCTTT+TGG 0.310244 8.2:-9452890 MS.gene68584:CDS
TTGTATACTGCCATCACTAT+TGG 0.316902 8.2:+9453610 None:intergenic
TGGACCTATTGGAAGCTATA+TGG 0.318011 8.2:-9452585 MS.gene68584:CDS
AGGGACAGGTTGTGCACCTT+TGG 0.336201 8.2:+9454820 None:intergenic
AGGACTGTTAAGCTTCTCTT+TGG 0.339704 8.2:+9455016 None:intergenic
GCCTTAGCAATTGCCCTTTG+TGG 0.342396 8.2:-9454902 MS.gene68584:CDS
GCTCTTGCCATATCATTATC+TGG 0.347069 8.2:-9454647 MS.gene68584:CDS
TGATATTCATCCTCTTGTTA+TGG 0.348410 8.2:-9452501 MS.gene68584:CDS
CACTCCACAATTGTTCATCT+TGG 0.352984 8.2:+9452783 None:intergenic
TATCTATGCAATGCACTTGA+TGG 0.358211 8.2:-9453425 MS.gene68584:CDS
AAGGTTGTTGTAATCTTCTT+CGG 0.366064 8.2:-9453004 MS.gene68584:CDS
GTCCCTTTCCTTGTCTTCAT+GGG 0.366810 8.2:-9454803 MS.gene68584:CDS
CAAATGTAAAGCTCTTTAAC+AGG 0.373112 8.2:+9454500 None:intergenic
TGATATTGAGACAGCATTGA+TGG 0.373365 8.2:-9452690 MS.gene68584:CDS
TGTTAATGATGTTGCAGCTT+GGG 0.380742 8.2:-9454678 MS.gene68584:CDS
GTTATCTTTACTGCTTGCTT+TGG 0.382323 8.2:-9454233 MS.gene68584:CDS
GATCATGAAGCACTTGCTTA+TGG 0.387350 8.2:-9452971 MS.gene68584:CDS
CACTAGTACAAATATCTTCA+TGG 0.402538 8.2:+9453224 None:intergenic
GTTGGTGTGTCTGGCACTTC+AGG 0.403487 8.2:+9452464 None:intergenic
TTCCTCGCTTTCGGGTTCCT+CGG 0.404658 8.2:+9453455 None:intergenic
TCCTCGCTTTCGGGTTCCTC+GGG 0.406728 8.2:+9453456 None:intergenic
GTTCCTATGCCAAGAACAAA+TGG 0.410938 8.2:+9454869 None:intergenic
AACTAACGTGTTGGTGTGTC+TGG 0.412667 8.2:+9452455 None:intergenic
CGTCGCCCCTGATTTCACTC+TGG 0.415870 8.2:-9454616 MS.gene68584:CDS
GCATAACCCAGAGTGAAATC+AGG 0.417320 8.2:+9454609 None:intergenic
CACTATTGGGGTGGTGATGA+AGG 0.425200 8.2:+9453624 None:intergenic
TTGTTCTTCTTATTCCTAGT+AGG 0.428237 8.2:-9454968 MS.gene68584:CDS
ACAACATGGAGTAAAATTCC+TGG 0.428599 8.2:+9452929 None:intergenic
TATAAGTGGAGGGGTTTCTT+GGG 0.428762 8.2:-9454270 MS.gene68584:CDS
CCAGCAAAAGGACCATCTTT+AGG 0.431587 8.2:+9454383 None:intergenic
TCTTTAACAGGTTCACCCTC+AGG 0.442719 8.2:+9454512 None:intergenic
TTACTAACCAATGGTGCAAC+CGG 0.444387 8.2:+9452626 None:intergenic
TGAAGAATCAATTAAGTATG+AGG 0.445267 8.2:-9452735 MS.gene68584:CDS
CTGAATCTTGATCTTTGCGT+TGG 0.445666 8.2:+9453548 None:intergenic
CCAAAAGACAATGTCATTCC+AGG 0.449037 8.2:+9452890 None:intergenic
CTGCTTGGGCCATCAGCAAT+AGG 0.449639 8.2:-9455070 MS.gene68584:CDS
CTGTTAATGATGTTGCAGCT+TGG 0.451241 8.2:-9454679 MS.gene68584:CDS
ACAAAATGATGAAGATCAAA+TGG 0.457736 8.2:-9453326 MS.gene68584:CDS
TCTCATGAACACCAAGGGAT+TGG 0.457954 8.2:-9454138 MS.gene68584:CDS
AATGCCACCGGTTGCACCAT+TGG 0.458140 8.2:-9452633 MS.gene68584:CDS
AACAGTGATTCAGCTGAACT+TGG 0.462781 8.2:-9452605 MS.gene68584:CDS
GGACTGTTAAGCTTCTCTTT+GGG 0.464658 8.2:+9455017 None:intergenic
GATGGAACCATGGAGTCATT+AGG 0.464824 8.2:-9453145 MS.gene68584:CDS
GTCGCCCCTGATTTCACTCT+GGG 0.466919 8.2:-9454615 MS.gene68584:CDS
TCGCTTCGACCTATTGCTGA+TGG 0.467719 8.2:+9455061 None:intergenic
AGTCATGCACCTTGTTCTGT+TGG 0.468441 8.2:-9453088 MS.gene68584:CDS
ATGCGTTCAATCCGTAGAAC+AGG 0.468555 8.2:-9454932 MS.gene68584:CDS
AAGACCGTTGCTTTCATACA+TGG 0.468874 8.2:-9454546 MS.gene68584:CDS
GTTATCGCTTTCCAAACCTA+TGG 0.474315 8.2:-9453304 MS.gene68584:CDS
TTTAGAGAAGCTCTTGCACT+TGG 0.475505 8.2:-9454164 MS.gene68584:CDS
TAAGGTGCTCCTCTTCGAGC+TGG 0.478801 8.2:+9453586 None:intergenic
GGTGCAGGACATGTCACATT+AGG 0.480678 8.2:+9455808 None:intergenic
GAAGCCATATAGCTTCCAAT+AGG 0.487421 8.2:+9452581 None:intergenic
GAACACAAATTGCAAATGCT+TGG 0.489528 8.2:+9453665 None:intergenic
AAGAGTAATTTGATATTGGT+TGG 0.496000 8.2:-9452659 MS.gene68584:CDS
ACAAATGTGGCTACTATAAG+TGG 0.497636 8.2:-9454284 MS.gene68584:CDS
AAGATTGAAGATCTTGTGTC+TGG 0.498090 8.2:-9454347 MS.gene68584:CDS
CAATGTTGGCTAGTGTGTCT+AGG 0.501202 8.2:+9454996 None:intergenic
TTGTGCACCTTTGGAGATTG+TGG 0.501442 8.2:+9454829 None:intergenic
TGGGAATGTGATTCCACAAA+GGG 0.501645 8.2:+9454889 None:intergenic
AGATTCAGAGCTCAGAGTCT+TGG 0.507048 8.2:-9453533 MS.gene68584:CDS
CCGTTAGACGTGGCCTTCAT+CGG 0.507520 8.2:+9455787 None:intergenic
CACCAAGATTGATGCAGAAG+TGG 0.507651 8.2:+9452547 None:intergenic
AGCTAGGATTCGAGCGAGGA+CGG 0.508099 8.2:+9454754 None:intergenic
CCGATGAAGGCCACGTCTAA+CGG 0.510024 8.2:-9455787 MS.gene68584:CDS
GACGTGGCCTTCATCGGTGC+AGG 0.510090 8.2:+9455793 None:intergenic
TCAATCAAAACACCAGCAAA+AGG 0.510996 8.2:+9454371 None:intergenic
TCCACAAAGGGCAATTGCTA+AGG 0.512792 8.2:+9454901 None:intergenic
GAAGAACAAAAGACCAATGT+TGG 0.513906 8.2:+9454982 None:intergenic
TGCAGGGAGGAATACTGCTT+GGG 0.514991 8.2:-9455084 MS.gene68584:intron
GTCGTCGCCAGATAATGATA+TGG 0.518435 8.2:+9454640 None:intergenic
CATTTGTATCACATTGACAT+TGG 0.519281 8.2:-9454483 MS.gene68584:CDS
TGTCGAGTTCAAGACCTACT+AGG 0.520539 8.2:+9454954 None:intergenic
ATGCAGGGAGGAATACTGCT+TGG 0.520657 8.2:-9455085 MS.gene68584:intron
GCTAGGATTCGAGCGAGGAC+GGG 0.524066 8.2:+9454755 None:intergenic
GGTATAATTATGCCTAAAGA+TGG 0.525746 8.2:-9454395 MS.gene68584:CDS
TTCCCATTTGTTCTTGGCAT+AGG 0.530710 8.2:-9454872 MS.gene68584:CDS
CTTTAACAGGTTCACCCTCA+GGG 0.537753 8.2:+9454513 None:intergenic
ATGAGAATGGCAGAACATCC+AGG 0.538151 8.2:-9452947 MS.gene68584:CDS
GAGAGCAGAAATTGCTGTCA+TGG 0.538200 8.2:+9453255 None:intergenic
GTAAAGTTCATTTCTCCACC+TGG 0.538553 8.2:-9452908 MS.gene68584:CDS
TTCCTATGCCAAGAACAAAT+GGG 0.538714 8.2:+9454870 None:intergenic
CTACAAGGTTGATTAGTGTA+GGG 0.540493 8.2:+9453485 None:intergenic
TCTACAAGGTTGATTAGTGT+AGG 0.546671 8.2:+9453484 None:intergenic
AAACTACTAACCACTGATGT+TGG 0.547854 8.2:-9454725 MS.gene68584:CDS
ACCAAAATTCCAACAGAACA+AGG 0.549877 8.2:+9453079 None:intergenic
AATTAAGTATGAGGAAAGGA+TGG 0.549955 8.2:-9452726 MS.gene68584:CDS
ACACCCATGAAGACAAGGAA+AGG 0.553825 8.2:+9454800 None:intergenic
GTATACTGCCATCACTATTG+GGG 0.558105 8.2:+9453612 None:intergenic
CACCCATGAAGACAAGGAAA+GGG 0.558235 8.2:+9454801 None:intergenic
CATAACCCAGAGTGAAATCA+GGG 0.561076 8.2:+9454610 None:intergenic
CATAAACACCAGCGTATCGA+TGG 0.563693 8.2:-9453163 MS.gene68584:CDS
ATACGCTGGTGTTTATGGAA+TGG 0.565041 8.2:+9453169 None:intergenic
CTCAGTATCAATGCTATGCA+AGG 0.565314 8.2:-9454192 MS.gene68584:CDS
GATATTGAAGGTAGCCATGA+TGG 0.569712 8.2:-9452815 MS.gene68584:CDS
ATGGCTAAGCGTTCCCCTGA+GGG 0.570360 8.2:-9454527 MS.gene68584:CDS
TGAATGTCCTAATGACTCCA+TGG 0.570671 8.2:+9453138 None:intergenic
AATGAGCTCAACCAATCCCT+TGG 0.575721 8.2:+9454127 None:intergenic
TGCATGAATTCCAATAGTAT+CGG 0.577236 8.2:+9454439 None:intergenic
AGAGCAGAAATTGCTGTCAT+GGG 0.578202 8.2:+9453256 None:intergenic
TCCAATTCCTCCATAACAAG+AGG 0.579300 8.2:+9452491 None:intergenic
ACTTGCTTATGGTATGAGAA+TGG 0.579368 8.2:-9452960 MS.gene68584:CDS
GTTGTTTGAGTGGTTTGCAA+AGG 0.579896 8.2:+9455671 None:intergenic
GTTGTGATTGATGATATTGA+AGG 0.581543 8.2:-9452827 MS.gene68584:CDS
TCTTTGGGAAAACTGTGTGT+AGG 0.583416 8.2:+9455032 None:intergenic
AAAACCAAGATGAACAATTG+TGG 0.583535 8.2:-9452787 MS.gene68584:CDS
TATTCATCCTCTTGTTATGG+AGG 0.583822 8.2:-9452498 MS.gene68584:CDS
GACATGTCCTGCACCGATGA+AGG 0.584379 8.2:-9455800 MS.gene68584:CDS
GCTGGTGTTTATGGAATGGA+AGG 0.586936 8.2:+9453173 None:intergenic
TCACTCTGGGTTATGCTCTG+TGG 0.589152 8.2:-9454602 MS.gene68584:CDS
GAGCAACACCCATGAAGACA+AGG 0.593494 8.2:+9454795 None:intergenic
GGATTTCTCATGAACACCAA+GGG 0.596547 8.2:-9454143 MS.gene68584:CDS
CTAGACACACTAGCCAACAT+TGG 0.598588 8.2:-9454995 MS.gene68584:CDS
ATGAAGACAAGGAAAGGGAC+AGG 0.600424 8.2:+9454806 None:intergenic
TAGTGGTTTGAAGACAAATG+TGG 0.600827 8.2:-9454297 MS.gene68584:CDS
ATGACTCGAGGTTGTTTGAG+TGG 0.603036 8.2:+9455661 None:intergenic
GAATCACTGTTACTAACCAA+TGG 0.603558 8.2:+9452617 None:intergenic
TCCCGAGGAACCCGAAAGCG+AGG 0.605796 8.2:-9453457 MS.gene68584:CDS
GATGAAATCAACTAACGTGT+TGG 0.605876 8.2:+9452446 None:intergenic
CCATGGTTCCATCGATACGC+TGG 0.609696 8.2:+9453155 None:intergenic
GAGCTCATTGTTCTCAACAT+CGG 0.610796 8.2:-9454113 MS.gene68584:CDS
AATCAATTAAGTATGAGGAA+AGG 0.611444 8.2:-9452730 MS.gene68584:CDS
TTAGTAGTTTGAGCTCAGCT+AGG 0.611873 8.2:+9454738 None:intergenic
GTTGTTGTAATCTTCTTCGG+AGG 0.614692 8.2:-9453001 MS.gene68584:CDS
TTGGTTGATCGCGGTCTCGG+AGG 0.615743 8.2:-9453061 MS.gene68584:CDS
ATTGGTTGGTAGAATGCCAC+CGG 0.617567 8.2:-9452645 MS.gene68584:CDS
CATCACCACCCCAATAGTGA+TGG 0.619210 8.2:-9453620 MS.gene68584:CDS
ATGATTTCTGCAATGACTCG+AGG 0.619902 8.2:+9455649 None:intergenic
TAGGGATGTTGCGTGTGCTG+TGG 0.621846 8.2:+9453503 None:intergenic
TTCCTCGGGATGATTCTACA+AGG 0.623083 8.2:+9453470 None:intergenic
ATGTGGCTACTATAAGTGGA+GGG 0.627903 8.2:-9454280 MS.gene68584:CDS
CTTAGAGCCACAATCTCCAA+AGG 0.629193 8.2:-9454836 MS.gene68584:CDS
TGTGGCTACTATAAGTGGAG+GGG 0.631044 8.2:-9454279 MS.gene68584:CDS
GTTGCGTGCTTTGTGAACCA+TGG 0.633464 8.2:+9453375 None:intergenic
CCACTAGAAGCAAAATAGAG+TGG 0.635208 8.2:+9454314 None:intergenic
GTTAATTCTTCAGATATGTG+TGG 0.635681 8.2:-9455722 MS.gene68584:CDS
AATGTGGCTACTATAAGTGG+AGG 0.637657 8.2:-9454281 MS.gene68584:CDS
AACGGAAGTGCATAATCGAG+AGG 0.638478 8.2:+9455745 None:intergenic
TTGGAATGCACCGTTAGACG+TGG 0.639960 8.2:+9455777 None:intergenic
CCAGCGTATCGATGGAACCA+TGG 0.641905 8.2:-9453155 MS.gene68584:CDS
TGGATTTCTCATGAACACCA+AGG 0.642435 8.2:-9454144 MS.gene68584:CDS
GTTCACAAAGCACGCAACAA+TGG 0.642568 8.2:-9453370 MS.gene68584:CDS
TGCTATACGACCAACATCAG+TGG 0.645855 8.2:+9454715 None:intergenic
ATGGGAATGTGATTCCACAA+AGG 0.646197 8.2:+9454888 None:intergenic
CATTGTTCTCAACATCGGAA+AGG 0.647673 8.2:-9454108 MS.gene68584:CDS
CAACATCGGAAAGGATCGCA+AGG 0.652219 8.2:-9454099 MS.gene68584:intron
CATGGCTAAGCGTTCCCCTG+AGG 0.653527 8.2:-9454528 MS.gene68584:CDS
CTAACCAATGGTGCAACCGG+TGG 0.654366 8.2:+9452629 None:intergenic
TTTAACAGGTTCACCCTCAG+GGG 0.666338 8.2:+9454514 None:intergenic
TTAGCCATGTATGAAAGCAA+CGG 0.666589 8.2:+9454542 None:intergenic
TACTGCCATCACTATTGGGG+TGG 0.669299 8.2:+9453615 None:intergenic
GAGAAATGAACTTTACAACA+TGG 0.677164 8.2:+9452915 None:intergenic
CATATCTGAAGAATTAACAA+CGG 0.681401 8.2:+9455727 None:intergenic
AAAGACAATGTCATTCCAGG+TGG 0.682922 8.2:+9452893 None:intergenic
GTATACAAACCAGCTCGAAG+AGG 0.689387 8.2:-9453595 MS.gene68584:CDS
GATTGTGGCTCTAAGAACAA+CGG 0.700703 8.2:+9454844 None:intergenic
GAATGGAAGGAGTATTATCG+CGG 0.711496 8.2:+9453186 None:intergenic
AACCTTGTAGAATCATCCCG+AGG 0.713913 8.2:-9453472 MS.gene68584:CDS
AAGATCTTCAGCTATCACCA+TGG 0.719007 8.2:-9453392 MS.gene68584:CDS
GCTCAGCTAGGATTCGAGCG+AGG 0.755526 8.2:+9454750 None:intergenic
ATAACCCAGAGTGAAATCAG+GGG 0.767323 8.2:+9454611 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATTAATCTTATAAAG+TGG - chr8.2:9452814-9452833 MS.gene68584:CDS 10.0%
!! AAAATATAACCATCAAATTA+AGG + chr8.2:9452722-9452741 None:intergenic 15.0%
!! AAATATAACCATCAAATTAA+GGG + chr8.2:9452721-9452740 None:intergenic 15.0%
!! TCAGAAAAGAATTAAAATAT+CGG - chr8.2:9454334-9454353 MS.gene68584:CDS 15.0%
!! TTGAAATATTTAACGATAAT+TGG + chr8.2:9454518-9454537 None:intergenic 15.0%
!!! ATTTTGTCATTAATTATTAG+AGG + chr8.2:9454462-9454481 None:intergenic 15.0%
!!! GTTTTATATTAAGATATAAG+TGG - chr8.2:9453065-9453084 MS.gene68584:CDS 15.0%
!! CAGAAAAGAATTAAAATATC+GGG - chr8.2:9454335-9454354 MS.gene68584:CDS 20.0%
!! TAATTATTAGAGGATCAATA+AGG + chr8.2:9454452-9454471 None:intergenic 20.0%
!! TTTCAAATCTTTCATTACTT+TGG - chr8.2:9454532-9454551 MS.gene68584:CDS 20.0%
!! TTTGATAAAACAACAAATTG+TGG + chr8.2:9452692-9452711 None:intergenic 20.0%
!!! ATTTTGTTTTTTGTTCCAAA+AGG + chr8.2:9454935-9454954 None:intergenic 20.0%
!!! TGTTTTGTTTTGTAAATTAG+TGG - chr8.2:9452959-9452978 MS.gene68584:CDS 20.0%
!!! TTTTGTTTTTTGTTCCAAAA+GGG + chr8.2:9454934-9454953 None:intergenic 20.0%
! AAATTGATATGTAAGCTAGT+CGG + chr8.2:9452938-9452957 None:intergenic 25.0%
! AACAAAATTGCACTGAAATA+TGG + chr8.2:9454308-9454327 None:intergenic 25.0%
! AAGAGTAATTTGATATTGGT+TGG - chr8.2:9455615-9455634 MS.gene68584:intron 25.0%
! AATCAATTAAGTATGAGGAA+AGG - chr8.2:9455544-9455563 MS.gene68584:intron 25.0%
! ACAAAATGATGAAGATCAAA+TGG - chr8.2:9454948-9454967 MS.gene68584:CDS 25.0%
! ATTGGAATTCATGCATTATT+TGG - chr8.2:9453843-9453862 MS.gene68584:intron 25.0%
! ATTTGCAATTTGTGTTCTTA+TGG - chr8.2:9454615-9454634 MS.gene68584:CDS 25.0%
! CATATCTGAAGAATTAACAA+CGG + chr8.2:9452550-9452569 None:intergenic 25.0%
! TGAAGAATCAATTAAGTATG+AGG - chr8.2:9455539-9455558 MS.gene68584:intron 25.0%
! TGGACTAATGATACAAAATA+GGG + chr8.2:9452672-9452691 None:intergenic 25.0%
!! CGATGTTGGTTATTATATTA+AGG - chr8.2:9453009-9453028 MS.gene68584:CDS 25.0%
!!! ACCTTTTGAATTGATTTTGA+GGG - chr8.2:9452904-9452923 MS.gene68584:CDS 25.0%
!!! TACCTTTTGAATTGATTTTG+AGG - chr8.2:9452903-9452922 MS.gene68584:CDS 25.0%
!!! TTATTTGGTGCTTTTGTTAT+TGG - chr8.2:9453858-9453877 MS.gene68584:intron 25.0%
!!! TTTATTTTCTTCCATATGCA+CGG + chr8.2:9452865-9452884 None:intergenic 25.0%
AAAACCAAGATGAACAATTG+TGG - chr8.2:9455487-9455506 MS.gene68584:intron 30.0%
AAGGTTGTTGTAATCTTCTT+CGG - chr8.2:9455270-9455289 MS.gene68584:intron 30.0%
AATTAAGTATGAGGAAAGGA+TGG - chr8.2:9455548-9455567 MS.gene68584:intron 30.0%
AGTTAGTGAGACAATGAATA+AGG - chr8.2:9452764-9452783 MS.gene68584:CDS 30.0%
ATGTGAAAATGTTCAATGCA+GGG - chr8.2:9453174-9453193 MS.gene68584:CDS 30.0%
CAAATGTAAAGCTCTTTAAC+AGG + chr8.2:9453777-9453796 None:intergenic 30.0%
CACTAGTACAAATATCTTCA+TGG + chr8.2:9455053-9455072 None:intergenic 30.0%
CATTTGTATCACATTGACAT+TGG - chr8.2:9453791-9453810 MS.gene68584:intron 30.0%
GAAGATTACAACAACCTTAA+AGG + chr8.2:9455268-9455287 None:intergenic 30.0%
GAGAAATGAACTTTACAACA+TGG + chr8.2:9455362-9455381 None:intergenic 30.0%
GAGCAAGAGTAATTTGATAT+TGG - chr8.2:9455611-9455630 MS.gene68584:intron 30.0%
GGACTAATGATACAAAATAG+GGG + chr8.2:9452671-9452690 None:intergenic 30.0%
GGATACTTCTAAGTATTACT+TGG + chr8.2:9454497-9454516 None:intergenic 30.0%
GGTATAATTATGCCTAAAGA+TGG - chr8.2:9453879-9453898 MS.gene68584:intron 30.0%
GTTAATTCTTCAGATATGTG+TGG - chr8.2:9452552-9452571 MS.gene68584:CDS 30.0%
TATGTGAAAATGTTCAATGC+AGG - chr8.2:9453173-9453192 MS.gene68584:CDS 30.0%
TCCCTCAAAATCAATTCAAA+AGG + chr8.2:9452908-9452927 None:intergenic 30.0%
TGATATTCATCCTCTTGTTA+TGG - chr8.2:9455773-9455792 MS.gene68584:CDS 30.0%
TGCATGAATTCCAATAGTAT+CGG + chr8.2:9453838-9453857 None:intergenic 30.0%
TTGTTCTTCTTATTCCTAGT+AGG - chr8.2:9453306-9453325 MS.gene68584:CDS 30.0%
! AAGAAAGTCCCTTAATTTGA+TGG - chr8.2:9452710-9452729 MS.gene68584:CDS 30.0%
! GTGGACTAATGATACAAAAT+AGG + chr8.2:9452673-9452692 None:intergenic 30.0%
! GTTGTGATTGATGATATTGA+AGG - chr8.2:9455447-9455466 MS.gene68584:intron 30.0%
! TGGACTGTTTTGTGTTTATA+AGG + chr8.2:9454709-9454728 None:intergenic 30.0%
! TGTTCAATTTTCCATAGGTT+TGG + chr8.2:9454984-9455003 None:intergenic 30.0%
! TTTGAAATCAAACGATGATG+TGG + chr8.2:9452841-9452860 None:intergenic 30.0%
!!! AGTTTTGTTACCGATACTAT+TGG - chr8.2:9453825-9453844 MS.gene68584:intron 30.0%
!!! ATTGTCTTTTGGTGCTAAAT+TGG - chr8.2:9455395-9455414 MS.gene68584:intron 30.0%
!!! TATCACATTGACATTGGTTT+TGG - chr8.2:9453797-9453816 MS.gene68584:intron 30.0%
AAACTACTAACCACTGATGT+TGG - chr8.2:9453549-9453568 MS.gene68584:CDS 35.0%
AAGATTGAAGATCTTGTGTC+TGG - chr8.2:9453927-9453946 MS.gene68584:intron 35.0%
ACAAATGTGGCTACTATAAG+TGG - chr8.2:9453990-9454009 MS.gene68584:intron 35.0%
ACAACATGGAGTAAAATTCC+TGG + chr8.2:9455348-9455367 None:intergenic 35.0%
ACCAAAATTCCAACAGAACA+AGG + chr8.2:9455198-9455217 None:intergenic 35.0%
ACTTGCTTATGGTATGAGAA+TGG - chr8.2:9455314-9455333 MS.gene68584:intron 35.0%
AGCAAGTGAAGTTTCCTTTA+AGG - chr8.2:9455251-9455270 MS.gene68584:intron 35.0%
ATCACATTCCCATTTGTTCT+TGG - chr8.2:9453396-9453415 MS.gene68584:CDS 35.0%
CATTTAGTACCTGTGTTACA+TGG + chr8.2:9454588-9454607 None:intergenic 35.0%
GAACACAAATTGCAAATGCT+TGG + chr8.2:9454612-9454631 None:intergenic 35.0%
GAAGAACAAAAGACCAATGT+TGG + chr8.2:9453295-9453314 None:intergenic 35.0%
GAATCACTGTTACTAACCAA+TGG + chr8.2:9455660-9455679 None:intergenic 35.0%
GTTATCTTTACTGCTTGCTT+TGG - chr8.2:9454041-9454060 MS.gene68584:intron 35.0%
TAGTGGTTTGAAGACAAATG+TGG - chr8.2:9453977-9453996 MS.gene68584:intron 35.0%
TATCTATGCAATGCACTTGA+TGG - chr8.2:9454849-9454868 MS.gene68584:CDS 35.0%
TCAATCAAAACACCAGCAAA+AGG + chr8.2:9453906-9453925 None:intergenic 35.0%
TGATTTCAAATCCGTGCATA+TGG - chr8.2:9452851-9452870 MS.gene68584:CDS 35.0%
TGTAATTCTCCATGTAACAC+AGG - chr8.2:9454576-9454595 MS.gene68584:CDS 35.0%
TGTATACTGCCATCACTATT+GGG + chr8.2:9454666-9454685 None:intergenic 35.0%
TGTCTCACTAACTTATTGCA+TGG + chr8.2:9452757-9452776 None:intergenic 35.0%
TGTTAATGATGTTGCAGCTT+GGG - chr8.2:9453596-9453615 MS.gene68584:CDS 35.0%
TTAGCCATGTATGAAAGCAA+CGG + chr8.2:9453735-9453754 None:intergenic 35.0%
TTCCTATGCCAAGAACAAAT+GGG + chr8.2:9453407-9453426 None:intergenic 35.0%
TTGTATACTGCCATCACTAT+TGG + chr8.2:9454667-9454686 None:intergenic 35.0%
! ACCTTGTTCTGTTGGAATTT+TGG - chr8.2:9455194-9455213 MS.gene68584:intron 35.0%
! CTACAAGGTTGATTAGTGTA+GGG + chr8.2:9454792-9454811 None:intergenic 35.0%
! GACACTGTTCAATTTTCCAT+AGG + chr8.2:9454989-9455008 None:intergenic 35.0%
! TATTCATCCTCTTGTTATGG+AGG - chr8.2:9455776-9455795 MS.gene68584:CDS 35.0%
! TCTACAAGGTTGATTAGTGT+AGG + chr8.2:9454793-9454812 None:intergenic 35.0%
! TGATATTGAGACAGCATTGA+TGG - chr8.2:9455584-9455603 MS.gene68584:intron 35.0%
!! GCTTGCTTTGGAAAAATTGT+TGG - chr8.2:9454053-9454072 MS.gene68584:intron 35.0%
!!! AGGCTTTTTTACCTGTTCTA+CGG + chr8.2:9453356-9453375 None:intergenic 35.0%
!!! TCTTTTGGTGCTAAATTGGT+TGG - chr8.2:9455399-9455418 MS.gene68584:intron 35.0%
AAAGACAATGTCATTCCAGG+TGG + chr8.2:9455384-9455403 None:intergenic 40.0%
AACAGTGATTCAGCTGAACT+TGG - chr8.2:9455669-9455688 MS.gene68584:CDS 40.0%
AAGATCTTCAGCTATCACCA+TGG - chr8.2:9454882-9454901 MS.gene68584:CDS 40.0%
AAGTGACAGCAACATTCAAC+AGG + chr8.2:9453141-9453160 None:intergenic 40.0%
AATGTGGCTACTATAAGTGG+AGG - chr8.2:9453993-9454012 MS.gene68584:intron 40.0%
AGAGCAGAAATTGCTGTCAT+GGG + chr8.2:9455021-9455040 None:intergenic 40.0%
AGGACTGTTAAGCTTCTCTT+TGG + chr8.2:9453261-9453280 None:intergenic 40.0%
AGTGCAAGAGCTTCTCTAAA+AGG + chr8.2:9454110-9454129 None:intergenic 40.0%
ATAACCCAGAGTGAAATCAG+GGG + chr8.2:9453666-9453685 None:intergenic 40.0%
ATACGCTGGTGTTTATGGAA+TGG + chr8.2:9455108-9455127 None:intergenic 40.0%
ATGATTTCTGCAATGACTCG+AGG + chr8.2:9452628-9452647 None:intergenic 40.0%
ATGGGAATGTGATTCCACAA+AGG + chr8.2:9453389-9453408 None:intergenic 40.0%
ATGTGGCTACTATAAGTGGA+GGG - chr8.2:9453994-9454013 MS.gene68584:intron 40.0%
CACTCCACAATTGTTCATCT+TGG + chr8.2:9455494-9455513 None:intergenic 40.0%
CATAACCCAGAGTGAAATCA+GGG + chr8.2:9453667-9453686 None:intergenic 40.0%
CATTCATGCTCTTTGCACTA+TGG + chr8.2:9455521-9455540 None:intergenic 40.0%
CATTGTTCTCAACATCGGAA+AGG - chr8.2:9454166-9454185 MS.gene68584:CDS 40.0%
CCAAAAGACAATGTCATTCC+AGG + chr8.2:9455387-9455406 None:intergenic 40.0%
CCACTAGAAGCAAAATAGAG+TGG + chr8.2:9453963-9453982 None:intergenic 40.0%
CGATCGAAATCTGGATAGAT+AGG + chr8.2:9454215-9454234 None:intergenic 40.0%
CTCAGTATCAATGCTATGCA+AGG - chr8.2:9454082-9454101 MS.gene68584:intron 40.0%
CTGAATCTTGATCTTTGCGT+TGG + chr8.2:9454729-9454748 None:intergenic 40.0%
CTGTTAATGATGTTGCAGCT+TGG - chr8.2:9453595-9453614 MS.gene68584:CDS 40.0%
GAAGCCATATAGCTTCCAAT+AGG + chr8.2:9455696-9455715 None:intergenic 40.0%
GAATAATCGTGCATCTACAC+AGG - chr8.2:9452981-9453000 MS.gene68584:CDS 40.0%
GACTATAAGCGATCGAAATC+TGG + chr8.2:9454224-9454243 None:intergenic 40.0%
GAGCTCATTGTTCTCAACAT+CGG - chr8.2:9454161-9454180 MS.gene68584:CDS 40.0%
GATATTGAAGGTAGCCATGA+TGG - chr8.2:9455459-9455478 MS.gene68584:intron 40.0%
GCATTTGGATCGAAATCAGA+CGG + chr8.2:9454374-9454393 None:intergenic 40.0%
GCTCTTGCCATATCATTATC+TGG - chr8.2:9453627-9453646 MS.gene68584:CDS 40.0%
GGACTGTTAAGCTTCTCTTT+GGG + chr8.2:9453260-9453279 None:intergenic 40.0%
GGATAGATAGGAAGACAGAT+AGG + chr8.2:9454203-9454222 None:intergenic 40.0%
GGATTTCTCATGAACACCAA+GGG - chr8.2:9454131-9454150 MS.gene68584:CDS 40.0%
GTAAAGTTCATTTCTCCACC+TGG - chr8.2:9455366-9455385 MS.gene68584:intron 40.0%
GTATACTGCCATCACTATTG+GGG + chr8.2:9454665-9454684 None:intergenic 40.0%
GTCAGTACATTAGTGGCATT+TGG + chr8.2:9454389-9454408 None:intergenic 40.0%
GTTATCGCTTTCCAAACCTA+TGG - chr8.2:9454970-9454989 MS.gene68584:CDS 40.0%
GTTCCTATGCCAAGAACAAA+TGG + chr8.2:9453408-9453427 None:intergenic 40.0%
GTTGTTGTAATCTTCTTCGG+AGG - chr8.2:9455273-9455292 MS.gene68584:intron 40.0%
TAAGGCAGTCAGTACATTAG+TGG + chr8.2:9454396-9454415 None:intergenic 40.0%
TACAAAGTCTTCCTCGCTTT+CGG + chr8.2:9454831-9454850 None:intergenic 40.0%
TATAAGTGGAGGGGTTTCTT+GGG - chr8.2:9454004-9454023 MS.gene68584:intron 40.0%
TCAGCAATCTCAAATCTCCA+TGG - chr8.2:9454242-9454261 MS.gene68584:CDS 40.0%
TCCAATTCCTCCATAACAAG+AGG + chr8.2:9455786-9455805 None:intergenic 40.0%
TGAAAATGTTCAATGCAGGG+AGG - chr8.2:9453177-9453196 MS.gene68584:CDS 40.0%
TGAATGTCCTAATGACTCCA+TGG + chr8.2:9455139-9455158 None:intergenic 40.0%
TGGACCTATTGGAAGCTATA+TGG - chr8.2:9455689-9455708 MS.gene68584:CDS 40.0%
TGGATTTCTCATGAACACCA+AGG - chr8.2:9454130-9454149 MS.gene68584:CDS 40.0%
TGGGAATGTGATTCCACAAA+GGG + chr8.2:9453388-9453407 None:intergenic 40.0%
TTACTAACCAATGGTGCAAC+CGG + chr8.2:9455651-9455670 None:intergenic 40.0%
TTAGTAGTTTGAGCTCAGCT+AGG + chr8.2:9453539-9453558 None:intergenic 40.0%
TTCCCATTTGTTCTTGGCAT+AGG - chr8.2:9453402-9453421 MS.gene68584:CDS 40.0%
TTTAGAGAAGCTCTTGCACT+TGG - chr8.2:9454110-9454129 MS.gene68584:CDS 40.0%
! AAGACCGTTGCTTTCATACA+TGG - chr8.2:9453728-9453747 MS.gene68584:intron 40.0%
! AATCGAGAGGATTTTCGTGT+TGG + chr8.2:9452519-9452538 None:intergenic 40.0%
! CCACTCTATTTTGCTTCTAG+TGG - chr8.2:9453960-9453979 MS.gene68584:intron 40.0%
! CCTGGAATGACATTGTCTTT+TGG - chr8.2:9455384-9455403 MS.gene68584:intron 40.0%
! GATCATGAAGCACTTGCTTA+TGG - chr8.2:9455303-9455322 MS.gene68584:intron 40.0%
! GTTGTTTGAGTGGTTTGCAA+AGG + chr8.2:9452606-9452625 None:intergenic 40.0%
! TCCTCTTGTTATGGAGGAAT+TGG - chr8.2:9455782-9455801 MS.gene68584:CDS 40.0%
! TCTTTGGGAAAACTGTGTGT+AGG + chr8.2:9453245-9453264 None:intergenic 40.0%
!! CTTGGTTTTTACTGCCATCA+TGG + chr8.2:9455476-9455495 None:intergenic 40.0%
!! GAATGGAAGGAGTATTATCG+CGG + chr8.2:9455091-9455110 None:intergenic 40.0%
!! GATTGTGGCTCTAAGAACAA+CGG + chr8.2:9453433-9453452 None:intergenic 40.0%
!!! GTTGGAATTTTGGTTGATCG+CGG - chr8.2:9455204-9455223 MS.gene68584:intron 40.0%
AACCTTGTAGAATCATCCCG+AGG - chr8.2:9454802-9454821 MS.gene68584:CDS 45.0%
AATGAGCTCAACCAATCCCT+TGG + chr8.2:9454150-9454169 None:intergenic 45.0%
ACAAAGTCTTCCTCGCTTTC+GGG + chr8.2:9454830-9454849 None:intergenic 45.0%
ACACCCATGAAGACAAGGAA+AGG + chr8.2:9453477-9453496 None:intergenic 45.0%
AGATTCAGAGCTCAGAGTCT+TGG - chr8.2:9454741-9454760 MS.gene68584:CDS 45.0%
AGCTAGCAATCCACGCATTA+AGG + chr8.2:9454414-9454433 None:intergenic 45.0%
AGTCATGCACCTTGTTCTGT+TGG - chr8.2:9455186-9455205 MS.gene68584:intron 45.0%
ATGAAGACAAGGAAAGGGAC+AGG + chr8.2:9453471-9453490 None:intergenic 45.0%
ATGACTCGAGGTTGTTTGAG+TGG + chr8.2:9452616-9452635 None:intergenic 45.0%
ATGAGAATGGCAGAACATCC+AGG - chr8.2:9455327-9455346 MS.gene68584:intron 45.0%
ATGCGTTCAATCCGTAGAAC+AGG - chr8.2:9453342-9453361 MS.gene68584:CDS 45.0%
CACCAAGATTGATGCAGAAG+TGG + chr8.2:9455730-9455749 None:intergenic 45.0%
CACCCATGAAGACAAGGAAA+GGG + chr8.2:9453476-9453495 None:intergenic 45.0%
CATAAACACCAGCGTATCGA+TGG - chr8.2:9455111-9455130 MS.gene68584:intron 45.0%
CATCGATACGCTGGTGTTTA+TGG + chr8.2:9455113-9455132 None:intergenic 45.0%
CCAGCAAAAGGACCATCTTT+AGG + chr8.2:9453894-9453913 None:intergenic 45.0%
CTAGACACACTAGCCAACAT+TGG - chr8.2:9453279-9453298 MS.gene68584:CDS 45.0%
CTATAAGTGGAGGGGTTTCT+TGG - chr8.2:9454003-9454022 MS.gene68584:intron 45.0%
CTCCACTTCTGCATCAATCT+TGG - chr8.2:9455725-9455744 MS.gene68584:CDS 45.0%
CTTAGAGCCACAATCTCCAA+AGG - chr8.2:9453438-9453457 MS.gene68584:CDS 45.0%
CTTTAACAGGTTCACCCTCA+GGG + chr8.2:9453764-9453783 None:intergenic 45.0%
GAGAGCAGAAATTGCTGTCA+TGG + chr8.2:9455022-9455041 None:intergenic 45.0%
GATGGAACCATGGAGTCATT+AGG - chr8.2:9455129-9455148 MS.gene68584:intron 45.0%
GCATAACCCAGAGTGAAATC+AGG + chr8.2:9453668-9453687 None:intergenic 45.0%
GCTGGTGTTTATGGAATGGA+AGG + chr8.2:9455104-9455123 None:intergenic 45.0%
GTATACAAACCAGCTCGAAG+AGG - chr8.2:9454679-9454698 MS.gene68584:CDS 45.0%
GTCCCTTTCCTTGTCTTCAT+GGG - chr8.2:9453471-9453490 MS.gene68584:CDS 45.0%
GTCGTCGCCAGATAATGATA+TGG + chr8.2:9453637-9453656 None:intergenic 45.0%
GTTCACAAAGCACGCAACAA+TGG - chr8.2:9454904-9454923 MS.gene68584:CDS 45.0%
TCAGCTGAACTTGGACCTAT+TGG - chr8.2:9455678-9455697 MS.gene68584:CDS 45.0%
TCCACAAAGGGCAATTGCTA+AGG + chr8.2:9453376-9453395 None:intergenic 45.0%
TCTCATGAACACCAAGGGAT+TGG - chr8.2:9454136-9454155 MS.gene68584:CDS 45.0%
TCTTTAACAGGTTCACCCTC+AGG + chr8.2:9453765-9453784 None:intergenic 45.0%
TGCTATACGACCAACATCAG+TGG + chr8.2:9453562-9453581 None:intergenic 45.0%
TGTCCCTTTCCTTGTCTTCA+TGG - chr8.2:9453470-9453489 MS.gene68584:CDS 45.0%
TGTCGAGTTCAAGACCTACT+AGG + chr8.2:9453323-9453342 None:intergenic 45.0%
TGTGGCTACTATAAGTGGAG+GGG - chr8.2:9453995-9454014 MS.gene68584:intron 45.0%
TTCCTCGGGATGATTCTACA+AGG + chr8.2:9454807-9454826 None:intergenic 45.0%
TTTAACAGGTTCACCCTCAG+GGG + chr8.2:9453763-9453782 None:intergenic 45.0%
! AACGGAAGTGCATAATCGAG+AGG + chr8.2:9452532-9452551 None:intergenic 45.0%
! CAATGTTGGCTAGTGTGTCT+AGG + chr8.2:9453281-9453300 None:intergenic 45.0%
! CCTAAAGATGGTCCTTTTGC+TGG - chr8.2:9453891-9453910 MS.gene68584:intron 45.0%
! GCAACAATGGAATGCCCTTT+TGG - chr8.2:9454917-9454936 MS.gene68584:CDS 45.0%
! TTGTGCACCTTTGGAGATTG+TGG + chr8.2:9453448-9453467 None:intergenic 45.0%
!! ATTGGTTGGTAGAATGCCAC+CGG - chr8.2:9455629-9455648 MS.gene68584:intron 45.0%
!!! ATTTTGGTTGATCGCGGTCT+CGG - chr8.2:9455210-9455229 MS.gene68584:intron 45.0%
ATGCAGGGAGGAATACTGCT+TGG - chr8.2:9453189-9453208 MS.gene68584:CDS 50.0%
CAACATCGGAAAGGATCGCA+AGG - chr8.2:9454175-9454194 MS.gene68584:CDS 50.0%
CATCACCACCCCAATAGTGA+TGG - chr8.2:9454654-9454673 MS.gene68584:CDS 50.0%
CTACACAGGTCTTGCGATGT+TGG - chr8.2:9452995-9453014 MS.gene68584:CDS 50.0%
GAGCAACACCCATGAAGACA+AGG + chr8.2:9453482-9453501 None:intergenic 50.0%
GCCTTAGCAATTGCCCTTTG+TGG - chr8.2:9453372-9453391 MS.gene68584:CDS 50.0%
GGTGCAGGACATGTCACATT+AGG + chr8.2:9452469-9452488 None:intergenic 50.0%
GTTGCGTGCTTTGTGAACCA+TGG + chr8.2:9454902-9454921 None:intergenic 50.0%
TACTGCCATCACTATTGGGG+TGG + chr8.2:9454662-9454681 None:intergenic 50.0%
TCACTCTGGGTTATGCTCTG+TGG - chr8.2:9453672-9453691 MS.gene68584:CDS 50.0%
TCGCTTCGACCTATTGCTGA+TGG + chr8.2:9453216-9453235 None:intergenic 50.0%
TGCAGGGAGGAATACTGCTT+GGG - chr8.2:9453190-9453209 MS.gene68584:CDS 50.0%
TTGGAATGCACCGTTAGACG+TGG + chr8.2:9452500-9452519 None:intergenic 50.0%
! CACTATTGGGGTGGTGATGA+AGG + chr8.2:9454653-9454672 None:intergenic 50.0%
!! GTACTGACTGCCTTAATGCG+TGG - chr8.2:9454401-9454420 MS.gene68584:CDS 50.0%
!! TCACTGATGTGCTGACTCCA+TGG + chr8.2:9454262-9454281 None:intergenic 50.0%
AGCTAGGATTCGAGCGAGGA+CGG + chr8.2:9453523-9453542 None:intergenic 55.0%
AGGGACAGGTTGTGCACCTT+TGG + chr8.2:9453457-9453476 None:intergenic 55.0%
ATGGCTAAGCGTTCCCCTGA+GGG - chr8.2:9453747-9453766 MS.gene68584:intron 55.0%
CCATGGTTCCATCGATACGC+TGG + chr8.2:9455122-9455141 None:intergenic 55.0%
CCGATGAAGGCCACGTCTAA+CGG - chr8.2:9452487-9452506 MS.gene68584:CDS 55.0%
CCGTTAGACGTGGCCTTCAT+CGG + chr8.2:9452490-9452509 None:intergenic 55.0%
CTAACCAATGGTGCAACCGG+TGG + chr8.2:9455648-9455667 None:intergenic 55.0%
CTGCTTGGGCCATCAGCAAT+AGG - chr8.2:9453204-9453223 MS.gene68584:CDS 55.0%
GTCGCCCCTGATTTCACTCT+GGG - chr8.2:9453659-9453678 MS.gene68584:CDS 55.0%
GTTGGTGTGTCTGGCACTTC+AGG + chr8.2:9455813-9455832 None:intergenic 55.0%
TAGGGATGTTGCGTGTGCTG+TGG + chr8.2:9454774-9454793 None:intergenic 55.0%
! AATGCCACCGGTTGCACCAT+TGG - chr8.2:9455641-9455660 MS.gene68584:intron 55.0%
! CCAGCGTATCGATGGAACCA+TGG - chr8.2:9455119-9455138 MS.gene68584:intron 55.0%
! GACATGTCCTGCACCGATGA+AGG - chr8.2:9452474-9452493 MS.gene68584:CDS 55.0%
! TAAGGTGCTCCTCTTCGAGC+TGG + chr8.2:9454691-9454710 None:intergenic 55.0%
!! TTCCTCGCTTTCGGGTTCCT+CGG + chr8.2:9454822-9454841 None:intergenic 55.0%
CATGGCTAAGCGTTCCCCTG+AGG - chr8.2:9453746-9453765 MS.gene68584:intron 60.0%
CGTCGCCCCTGATTTCACTC+TGG - chr8.2:9453658-9453677 MS.gene68584:CDS 60.0%
GCTAGGATTCGAGCGAGGAC+GGG + chr8.2:9453522-9453541 None:intergenic 60.0%
GCTCAGCTAGGATTCGAGCG+AGG + chr8.2:9453527-9453546 None:intergenic 60.0%
TTGGTTGATCGCGGTCTCGG+AGG - chr8.2:9455213-9455232 MS.gene68584:intron 60.0%
!! TCCTCGCTTTCGGGTTCCTC+GGG + chr8.2:9454821-9454840 None:intergenic 60.0%
GACGTGGCCTTCATCGGTGC+AGG + chr8.2:9452484-9452503 None:intergenic 65.0%
TCCCGAGGAACCCGAAAGCG+AGG - chr8.2:9454817-9454836 MS.gene68584:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 9452457 9455839 9452457 ID=MS.gene68584
chr8.2 mRNA 9452457 9455839 9452457 ID=MS.gene68584.t1;Parent=MS.gene68584
chr8.2 exon 9455648 9455839 9455648 ID=MS.gene68584.t1.exon1;Parent=MS.gene68584.t1
chr8.2 CDS 9455648 9455839 9455648 ID=cds.MS.gene68584.t1;Parent=MS.gene68584.t1
chr8.2 exon 9454100 9455101 9454100 ID=MS.gene68584.t1.exon2;Parent=MS.gene68584.t1
chr8.2 CDS 9454100 9455101 9454100 ID=cds.MS.gene68584.t1;Parent=MS.gene68584.t1
chr8.2 exon 9452457 9453698 9452457 ID=MS.gene68584.t1.exon3;Parent=MS.gene68584.t1
chr8.2 CDS 9452457 9453698 9452457 ID=cds.MS.gene68584.t1;Parent=MS.gene68584.t1
Gene Sequence

>MS.gene68584

ATGGCGACGCCTAATGTGACATGTCCTGCACCGATGAAGGCCACGTCTAACGGTGCATTCCAACACGAAAATCCTCTCGATTATGCACTTCCGTTGTTAATTCTTCAGATATGTGTGGTTGTCACATTCACTAGATGTATTGCATACCTTTGCAAACCACTCAAACAACCTCGAGTCATTGCAGAAATCATTGTAAGTTGTCTTCTCTTTTCCCCTATTTTGTATCATTAGTCCACAATTTGTTGTTTTATCAAAGAAAGTCCCTTAATTTGATGGTTATATTTTATATTTGAGTTTCCATGCAATAAGTTAGTGAGACAATGAATAAGGCAGTGTGACATGATTGACAAATTATTATAATTATTAATCTTATAAAGTGGTCCACATCATCGTTTGATTTCAAATCCGTGCATATGGAAGAAAATAAAAATTGTCAATGAGAAAATTACCTTTTGAATTGATTTTGAGGGATTAGTCTCCGACTAGCTTACATATCAATTTTTGTTTTGTTTTGTAAATTAGTGGAATAATCGTGCATCTACACAGGTCTTGCGATGTTGGTTATTATATTAAGGTAGTTAATATAAGATTAAAATGTTTAGAATTTTGTTTTATATTAAGATATAAGTGGAATTATTGAAACGTAATTTCTTCTGTTGCAAGTAAAACTTTTGTATGTTGCCTGTTGAATGTTGCTGTCACTTTTGTAAGTTTCTTATGTGAAAATGTTCAATGCAGGGAGGAATACTGCTTGGGCCATCAGCAATAGGTCGAAGCGAGAAATTCCTACACACAGTTTTCCCAAAGAGAAGCTTAACAGTCCTAGACACACTAGCCAACATTGGTCTTTTGTTCTTCTTATTCCTAGTAGGTCTTGAACTCGACATGCGTTCAATCCGTAGAACAGGTAAAAAAGCCTTAGCAATTGCCCTTTGTGGAATCACATTCCCATTTGTTCTTGGCATAGGAACATCCGTTGTTCTTAGAGCCACAATCTCCAAAGGTGCACAACCTGTCCCTTTCCTTGTCTTCATGGGTGTTGCTCTCTCAATCACAGCATTTCCCGTCCTCGCTCGAATCCTAGCTGAGCTCAAACTACTAACCACTGATGTTGGTCGTATAGCAATGTCAGCAGCTGCTGTTAATGATGTTGCAGCTTGGGTACTTCTTGCTCTTGCCATATCATTATCTGGCGACGACACGTCGCCCCTGATTTCACTCTGGGTTATGCTCTGTGGTGCTGCTTTTATTCTCTTTGCTGTTTTTGCTATAAGACCGTTGCTTTCATACATGGCTAAGCGTTCCCCTGAGGGTGAACCTGTTAAAGAGCTTTACATTTGTATCACATTGACATTGGTTTTGGCTTGTAGTTTTGTTACCGATACTATTGGAATTCATGCATTATTTGGTGCTTTTGTTATTGGTATAATTATGCCTAAAGATGGTCCTTTTGCTGGTGTTTTGATTGAGAAGATTGAAGATCTTGTGTCTGGAATCTTTTTGCCACTCTATTTTGCTTCTAGTGGTTTGAAGACAAATGTGGCTACTATAAGTGGAGGGGTTTCTTGGGCACTATTATTACTAGTTATCTTTACTGCTTGCTTTGGAAAAATTGTTGGTACAATCTCAGTATCAATGCTATGCAAGGTGCCTTTTAGAGAAGCTCTTGCACTTGGATTTCTCATGAACACCAAGGGATTGGTTGAGCTCATTGTTCTCAACATCGGAAAGGATCGCAAGGTACCTATCTGTCTTCCTATCTATCCAGATTTCGATCGCTTATAGTCAGCAATCTCAAATCTCCATGGAGTCAGCACATCAGTGACTTTTCGATCGAGATCAGACTATCCATATTTCAGTGCAATTTTGTTGATTTTTCAGAAAAGAATTAAAATATCGGGTCTGAAGTTTGAACCGTCTGATTTCGATCCAAATGCCACTAATGTACTGACTGCCTTAATGCGTGGATTGCTAGCTGCAGACAATCTAAATCCTTATTGATCCTCTAATAATTAATGACAAAATAAAACTTGTTTTCCAAGTAATACTTAGAAGTATCCAATTATCGTTAAATATTTCAAATCTTTCATTACTTTGGTTTGATTTTTTATGCATGTAATGTAATTCTCCATGTAACACAGGTACTAAATGACCAAGCATTTGCAATTTGTGTTCTTATGGCATTATTCACTACCTTCATCACCACCCCAATAGTGATGGCAGTATACAAACCAGCTCGAAGAGGAGCACCTTATAAACACAAAACAGTCCAACGCAAAGATCAAGATTCAGAGCTCAGAGTCTTGGCATGTTTCCACAGCACACGCAACATCCCTACACTAATCAACCTTGTAGAATCATCCCGAGGAACCCGAAAGCGAGGAAGACTTTGTATCTATGCAATGCACTTGATGGAACTCTCAGAAAGATCTTCAGCTATCACCATGGTTCACAAAGCACGCAACAATGGAATGCCCTTTTGGAACAAAAAACAAAATGATGAAGATCAAATGGTTATCGCTTTCCAAACCTATGGAAAATTGAACAGTGTCAATGTTCGTCCCATGACAGCAATTTCTGCTCTCAGCAACATCCATGAAGATATTTGTACTAGTGCTCATCAAAAGCGCGCCGCGATAATACTCCTTCCATTCCATAAACACCAGCGTATCGATGGAACCATGGAGTCATTAGGACATTCATTTCATGTAATCAATGAGCTTGTGTTGAGTCATGCACCTTGTTCTGTTGGAATTTTGGTTGATCGCGGTCTCGGAGGAACAAGTCAAGTTCAAGCAAGTGAAGTTTCCTTTAAGGTTGTTGTAATCTTCTTCGGAGGACGCGACGATCATGAAGCACTTGCTTATGGTATGAGAATGGCAGAACATCCAGGAATTTTACTCCATGTTGTAAAGTTCATTTCTCCACCTGGAATGACATTGTCTTTTGGTGCTAAATTGGTTGGTGTAGCACAAGATAAGAGCAAAACAGTTGTGATTGATGATATTGAAGGTAGCCATGATGGCAGTAAAAACCAAGATGAACAATTGTGGAGTGAATTCCATAGTGCAAAGAGCATGAATGAAGAATCAATTAAGTATGAGGAAAGGATGGTTGAAAGTAAAAATGATATTGAGACAGCATTGATGGAATTGAGCAAGAGTAATTTGATATTGGTTGGTAGAATGCCACCGGTTGCACCATTGGTTAGTAACAGTGATTCAGCTGAACTTGGACCTATTGGAAGCTATATGGCTTCTTCTAGTTTCTCCACTTCTGCATCAATCTTGGTGATTCAACAATATAATTCAACAACTGATATTCATCCTCTTGTTATGGAGGAATTGGATTGTCCTGAAGTGCCAGACACACCAACACGTTAG
Protein sequence

>MS.gene68584.t1

MATPNVTCPAPMKATSNGAFQHENPLDYALPLLILQICVVVTFTRCIAYLCKPLKQPRVIAEIIGGILLGPSAIGRSEKFLHTVFPKRSLTVLDTLANIGLLFFLFLVGLELDMRSIRRTGKKALAIALCGITFPFVLGIGTSVVLRATISKGAQPVPFLVFMGVALSITAFPVLARILAELKLLTTDVGRIAMSAAAVNDVAAWVLLALAISLSGDDTSPLISLWVMLCGAAFILFAVFAIRPLLSYMAKRSPEGEPVKELYICITLTLVLACSFVTDTIGIHALFGAFVIGIIMPKDGPFAGVLIEKIEDLVSGIFLPLYFASSGLKTNVATISGGVSWALLLLVIFTACFGKIVGTISVSMLCKVPFREALALGFLMNTKGLVELIVLNIGKDRKVLNDQAFAICVLMALFTTFITTPIVMAVYKPARRGAPYKHKTVQRKDQDSELRVLACFHSTRNIPTLINLVESSRGTRKRGRLCIYAMHLMELSERSSAITMVHKARNNGMPFWNKKQNDEDQMVIAFQTYGKLNSVNVRPMTAISALSNIHEDICTSAHQKRAAIILLPFHKHQRIDGTMESLGHSFHVINELVLSHAPCSVGILVDRGLGGTSQVQASEVSFKVVVIFFGGRDDHEALAYGMRMAEHPGILLHVVKFISPPGMTLSFGAKLVGVAQDKSKTVVIDDIEGSHDGSKNQDEQLWSEFHSAKSMNEESIKYEERMVESKNDIETALMELSKSNLILVGRMPPVAPLVSNSDSAELGPIGSYMASSSFSTSASILVIQQYNSTTDIHPLVMEELDCPEVPDTPTR