Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073780.t1 | XP_013468235.1 | 65.1 | 86 | 2 | 1 | 1 | 58 | 141 | 226 | 3.50E-18 | 100.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073780.t1 | Q6NLA5 | 53.5 | 86 | 11 | 2 | 1 | 58 | 142 | 226 | 2.2e-13 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073780.t1 | A0A072VKE3 | 65.1 | 86 | 2 | 1 | 1 | 58 | 141 | 226 | 2.5e-18 | 100.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049861 | MS.gene073780 | 0.84102 | 6.12E-58 | -1.69E-46 |
| MS.gene050288 | MS.gene073780 | 0.806734 | 7.12E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene073780 | 0.819085 | 1.40E-52 | -1.69E-46 |
| MS.gene050320 | MS.gene073780 | 0.801645 | 8.17E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene073780 | 0.801339 | 9.44E-49 | -1.69E-46 |
| MS.gene052818 | MS.gene073780 | -0.819434 | 1.16E-52 | -1.69E-46 |
| MS.gene056752 | MS.gene073780 | 0.814598 | 1.42E-51 | -1.69E-46 |
| MS.gene056764 | MS.gene073780 | 0.802975 | 4.35E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene073780 | 0.819975 | 8.76E-53 | -1.69E-46 |
| MS.gene057452 | MS.gene073780 | -0.803301 | 3.72E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene073780 | 0.825595 | 4.30E-54 | -1.69E-46 |
| MS.gene058238 | MS.gene073780 | 0.827428 | 1.57E-54 | -1.69E-46 |
| MS.gene060869 | MS.gene073780 | 0.808787 | 2.61E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073780.t1 | MTR_1g066770 | 65.116 | 86 | 2 | 1 | 1 | 58 | 141 | 226 | 4.38e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073780.t1 | AT5G03080 | 53.488 | 86 | 11 | 2 | 1 | 58 | 142 | 226 | 4.23e-20 | 79.3 |
Find 6 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGTATTCTAGGGTTTATT+TGG | 0.180795 | 1.1:+49218411 | None:intergenic |
| GTTATTGAAGAAAGTGCTTT+TGG | 0.264951 | 1.1:+49218538 | MS.gene073780:intron |
| ATGTATTCTAGGGTTTATTT+GGG | 0.378661 | 1.1:+49218412 | MS.gene073780:CDS |
| GGGTTATCATAGTGTTTCTC+AGG | 0.389344 | 1.1:+49218432 | MS.gene073780:CDS |
| TAGAGCTGCTAGAAAGAAGA+TGG | 0.522290 | 1.1:+49218627 | MS.gene073780:CDS |
| TGCTAGAAAGAAGATGGTCT+CGG | 0.534307 | 1.1:+49218633 | MS.gene073780:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTTCAATAACAGGAAAAAA+AGG | - | chr1.1:49218529-49218548 | None:intergenic | 25.0% |
| !!! | ATGTATTCTAGGGTTTATTT+GGG | + | chr1.1:49218412-49218431 | MS.gene073780:CDS | 25.0% |
| ! | AAAGCACTTTCTTCAATAAC+AGG | - | chr1.1:49218538-49218557 | None:intergenic | 30.0% |
| !!! | GTTATTGAAGAAAGTGCTTT+TGG | + | chr1.1:49218538-49218557 | MS.gene073780:intron | 30.0% |
| ! | CTAGTTTGGGGGTTTTTATT+GGG | + | chr1.1:49218467-49218486 | MS.gene073780:intron | 35.0% |
| ! | TAGTTTGGGGGTTTTTATTG+GGG | + | chr1.1:49218468-49218487 | MS.gene073780:intron | 35.0% |
| !! | ACTAGTTTGGGGGTTTTTAT+TGG | + | chr1.1:49218466-49218485 | MS.gene073780:intron | 35.0% |
| !! | GTTTTTGCTGGTACTAGTTT+GGG | + | chr1.1:49218454-49218473 | MS.gene073780:intron | 35.0% |
| !! | TTTTTGCTGGTACTAGTTTG+GGG | + | chr1.1:49218455-49218474 | MS.gene073780:intron | 35.0% |
| !!! | TGGAAGGTGGTTTTATGTTA+AGG | + | chr1.1:49218558-49218577 | MS.gene073780:CDS | 35.0% |
| !!! | TTGAAGAAAGTGCTTTTGGA+AGG | + | chr1.1:49218542-49218561 | MS.gene073780:intron | 35.0% |
| GGGTTATCATAGTGTTTCTC+AGG | + | chr1.1:49218432-49218451 | MS.gene073780:CDS | 40.0% | |
| TAGAGCTGCTAGAAAGAAGA+TGG | + | chr1.1:49218627-49218646 | MS.gene073780:CDS | 40.0% | |
| TGCTAGAAAGAAGATGGTCT+CGG | + | chr1.1:49218633-49218652 | MS.gene073780:CDS | 40.0% | |
| ! | AGTGTTTCTCAGGTTTTTGC+TGG | + | chr1.1:49218442-49218461 | MS.gene073780:CDS | 40.0% |
| ! | GGTTTTTGCTGGTACTAGTT+TGG | + | chr1.1:49218453-49218472 | MS.gene073780:intron | 40.0% |
| !! | TTTTGCTGGTACTAGTTTGG+GGG | + | chr1.1:49218456-49218475 | MS.gene073780:intron | 40.0% |
| !!! | AAGAAAGTGCTTTTGGAAGG+TGG | + | chr1.1:49218545-49218564 | MS.gene073780:intron | 40.0% |
| !! | TTATTGGGGCGCTTTGGTTT+TGG | + | chr1.1:49218482-49218501 | MS.gene073780:intron | 45.0% |
| !!! | TATTGGGGCGCTTTGGTTTT+GGG | + | chr1.1:49218483-49218502 | MS.gene073780:intron | 45.0% |
| !! | GGGTTTTTATTGGGGCGCTT+TGG | + | chr1.1:49218476-49218495 | MS.gene073780:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 49218412 | 49218672 | 49218412 | ID=MS.gene073780 |
| chr1.1 | mRNA | 49218412 | 49218672 | 49218412 | ID=MS.gene073780.t1;Parent=MS.gene073780 |
| chr1.1 | exon | 49218412 | 49218463 | 49218412 | ID=MS.gene073780.t1.exon1;Parent=MS.gene073780.t1 |
| chr1.1 | CDS | 49218412 | 49218463 | 49218412 | ID=cds.MS.gene073780.t1;Parent=MS.gene073780.t1 |
| chr1.1 | exon | 49218548 | 49218672 | 49218548 | ID=MS.gene073780.t1.exon2;Parent=MS.gene073780.t1 |
| chr1.1 | CDS | 49218548 | 49218672 | 49218548 | ID=cds.MS.gene073780.t1;Parent=MS.gene073780.t1 |
| Gene Sequence |
| Protein sequence |
