Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08362.t1 | RHN42315.1 | 97 | 164 | 3 | 1 | 1 | 164 | 3 | 164 | 5.30E-64 | 253.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08362.t1 | A0A396GU48 | 97.0 | 164 | 3 | 1 | 1 | 164 | 3 | 164 | 3.8e-64 | 253.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene08362 | 0.807423 | 5.09E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene08362 | 0.840782 | 7.07E-58 | -1.69E-46 |
| MS.gene052209 | MS.gene08362 | 0.804258 | 2.36E-49 | -1.69E-46 |
| MS.gene054850 | MS.gene08362 | 0.802525 | 5.38E-49 | -1.69E-46 |
| MS.gene054941 | MS.gene08362 | 0.808733 | 2.68E-50 | -1.69E-46 |
| MS.gene05652 | MS.gene08362 | 0.805422 | 1.35E-49 | -1.69E-46 |
| MS.gene057669 | MS.gene08362 | 0.821113 | 4.80E-53 | -1.69E-46 |
| MS.gene058128 | MS.gene08362 | 0.845914 | 3.02E-59 | -1.69E-46 |
| MS.gene058853 | MS.gene08362 | 0.83876 | 2.37E-57 | -1.69E-46 |
| MS.gene059616 | MS.gene08362 | 0.816353 | 5.78E-52 | -1.69E-46 |
| MS.gene059905 | MS.gene08362 | 0.840466 | 8.55E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08362.t1 | MTR_5g027940 | 32.110 | 109 | 71 | 2 | 14 | 120 | 17 | 124 | 2.12e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08362.t1 | AT1G31940 | 35.294 | 85 | 54 | 1 | 14 | 97 | 17 | 101 | 9.25e-13 | 62.8 |
| MS.gene08362.t1 | AT2G35585 | 32.558 | 86 | 57 | 1 | 14 | 98 | 17 | 102 | 9.16e-11 | 57.4 |
Find 44 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAAAATAGTGATATTC+TGG | 0.208224 | 4.1:+17117477 | None:intergenic |
| CGCTTTCGCACTCCTCGAGC+TGG | 0.260475 | 4.1:+17117584 | None:intergenic |
| GGCTCCATAGGATGTTCAAC+TGG | 0.393061 | 4.1:+17118453 | None:intergenic |
| AGGAAGAGAGGTGAGATGTC+TGG | 0.414508 | 4.1:-17117644 | MS.gene08362:CDS |
| GCTTTCGCACTCCTCGAGCT+GGG | 0.417036 | 4.1:+17117585 | None:intergenic |
| TTTCCCAGTTGAACATCCTA+TGG | 0.422933 | 4.1:-17118457 | MS.gene08362:CDS |
| ACACTAATGACTGAAGATTC+AGG | 0.437311 | 4.1:+17118396 | None:intergenic |
| ACCCTCACTCATGGATCATC+AGG | 0.442814 | 4.1:-17117554 | MS.gene08362:CDS |
| TTGGAAAATAGTGATATTCT+GGG | 0.444826 | 4.1:+17117478 | None:intergenic |
| TTTCCAAGTGCTTCAGCAGT+TGG | 0.454811 | 4.1:-17117462 | MS.gene08362:CDS |
| AGAGGTGAGATGTCTGGGTC+TGG | 0.464003 | 4.1:-17117638 | MS.gene08362:CDS |
| AGAGGAACACAGAAAGCATA+AGG | 0.478521 | 4.1:-17117664 | MS.gene08362:CDS |
| TGTCCAACTGCTGAAGCACT+TGG | 0.496596 | 4.1:+17117459 | None:intergenic |
| TCTTCATCCGGTGGCTCCAT+AGG | 0.504589 | 4.1:+17118441 | None:intergenic |
| GGGTCTGGACAAAGAACAGC+TGG | 0.506189 | 4.1:-17117623 | MS.gene08362:CDS |
| AGATGTGGCATTCTCATCAA+TGG | 0.507933 | 4.1:+17117515 | None:intergenic |
| ATATTCTGGGATTGTAGAGG+AGG | 0.508528 | 4.1:+17117491 | None:intergenic |
| CGGCATCACACCCTCACTCA+TGG | 0.508751 | 4.1:-17117563 | MS.gene08362:CDS |
| ATGACTGAAGATTCAGGCAT+TGG | 0.525006 | 4.1:+17118402 | None:intergenic |
| GCCACCGGATGAAGATCGTC+CGG | 0.527419 | 4.1:-17118433 | MS.gene08362:CDS |
| GGAAGAGAGGTGAGATGTCT+GGG | 0.529996 | 4.1:-17117643 | MS.gene08362:CDS |
| CAGCTCGAGGAGTGCGAAAG+CGG | 0.537056 | 4.1:-17117583 | MS.gene08362:CDS |
| TGGACAAAGAACAGCTGGCA+TGG | 0.537925 | 4.1:-17117618 | MS.gene08362:CDS |
| ATGAAAGGATGCATGAAAAG+AGG | 0.540753 | 4.1:-17117682 | MS.gene08362:CDS |
| TCAGGCATTGGACATTTCAC+CGG | 0.545910 | 4.1:+17118414 | None:intergenic |
| TATTCTGGGATTGTAGAGGA+GGG | 0.548828 | 4.1:+17117492 | None:intergenic |
| TTCACCGGACGATCTTCATC+CGG | 0.561909 | 4.1:+17118429 | None:intergenic |
| TACACAGAACAGTATCATCA+TGG | 0.562804 | 4.1:-17118535 | MS.gene08362:CDS |
| CTTTCGCACTCCTCGAGCTG+GGG | 0.565176 | 4.1:+17117586 | None:intergenic |
| TGAACATCCTATGGAGCCAC+CGG | 0.581194 | 4.1:-17118448 | MS.gene08362:CDS |
| ACAGAAAGCATAAGGAAGAG+AGG | 0.581626 | 4.1:-17117656 | MS.gene08362:CDS |
| ACTGTTCTGTGTAGTTACAC+TGG | 0.597328 | 4.1:+17118545 | None:intergenic |
| TCCTGATGATCCATGAGTGA+GGG | 0.599861 | 4.1:+17117553 | None:intergenic |
| GACACAGATGCCCCAGCTCG+AGG | 0.604247 | 4.1:-17117596 | MS.gene08362:CDS |
| ACCGGACGATCTTCATCCGG+TGG | 0.608873 | 4.1:+17118432 | None:intergenic |
| CCCTCTGATGATGATGGTGG+TGG | 0.609122 | 4.1:+17118499 | None:intergenic |
| GATTGTAGAGGAGGGAGATG+TGG | 0.615192 | 4.1:+17117500 | None:intergenic |
| GTGATATTCTGGGATTGTAG+AGG | 0.620846 | 4.1:+17117488 | None:intergenic |
| TATCAACCCTCTGATGATGA+TGG | 0.623161 | 4.1:+17118493 | None:intergenic |
| GCTCCATAGGATGTTCAACT+GGG | 0.634698 | 4.1:+17118454 | None:intergenic |
| ACCACCACCATCATCATCAG+AGG | 0.640128 | 4.1:-17118500 | MS.gene08362:CDS |
| CCACCACCATCATCATCAGA+GGG | 0.667838 | 4.1:-17118499 | MS.gene08362:CDS |
| CTCCTGATGATCCATGAGTG+AGG | 0.684933 | 4.1:+17117552 | None:intergenic |
| CAACCCTCTGATGATGATGG+TGG | 0.689596 | 4.1:+17118496 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTAATAATAAAACTAAA+AGG | - | chr4.1:17118128-17118147 | MS.gene08362:intron | 10.0% |
| !!! | CAACTAATTTTGTATATTTT+AGG | - | chr4.1:17117653-17117672 | MS.gene08362:CDS | 15.0% |
| !! | AACAATTATTGACAAATGTT+TGG | - | chr4.1:17118380-17118399 | MS.gene08362:intron | 20.0% |
| !! | AATTGGAGAATTTATTGATA+CGG | - | chr4.1:17117528-17117547 | MS.gene08362:CDS | 20.0% |
| !! | ATAAAGCATGAAGATAAAAA+TGG | + | chr4.1:17118441-17118460 | None:intergenic | 20.0% |
| !! | CAAAATGTCTAATGAATATT+TGG | + | chr4.1:17118247-17118266 | None:intergenic | 20.0% |
| !!! | CAAATATTCATTAGACATTT+TGG | - | chr4.1:17118245-17118264 | MS.gene08362:intron | 20.0% |
| !!! | TTATTTCACTAAAACTTGTT+TGG | - | chr4.1:17118457-17118476 | MS.gene08362:CDS | 20.0% |
| !!! | TTGTATATTTTAGGAAGAAA+AGG | - | chr4.1:17117662-17117681 | MS.gene08362:CDS | 20.0% |
| ! | AAGGAAATCAATATAGTTCT+AGG | + | chr4.1:17118339-17118358 | None:intergenic | 25.0% |
| ! | GACACATTATTATATGAACA+CGG | + | chr4.1:17118045-17118064 | None:intergenic | 25.0% |
| ! | TATTTGGATAGATATATCAC+AGG | + | chr4.1:17118231-17118250 | None:intergenic | 25.0% |
| ! | TTGGAAAATAGTGATATTCT+GGG | + | chr4.1:17118782-17118801 | None:intergenic | 25.0% |
| !! | AAAACTTGTTTGGATTCTTT+AGG | - | chr4.1:17118467-17118486 | MS.gene08362:CDS | 25.0% |
| !!! | TAGAACTATATTGATTTCCT+TGG | - | chr4.1:17118338-17118357 | MS.gene08362:intron | 25.0% |
| AATGTTTGGCAACAATTTAC+CGG | - | chr4.1:17118394-17118413 | MS.gene08362:intron | 30.0% | |
| CTTGGAAAATAGTGATATTC+TGG | + | chr4.1:17118783-17118802 | None:intergenic | 30.0% | |
| GTTACCTACTTATCTTAAGA+TGG | - | chr4.1:17117907-17117926 | MS.gene08362:intron | 30.0% | |
| TTCTTGTCCATTGATCAAAA+GGG | - | chr4.1:17117504-17117523 | MS.gene08362:CDS | 30.0% | |
| TTTCTTGTCCATTGATCAAA+AGG | - | chr4.1:17117503-17117522 | MS.gene08362:CDS | 30.0% | |
| TTTGCCATCTTAAGATAAGT+AGG | + | chr4.1:17117914-17117933 | None:intergenic | 30.0% | |
| ! | ATAATGTGTCACTAAACACT+AGG | - | chr4.1:17118055-17118074 | MS.gene08362:intron | 30.0% |
| ! | GACTAGAATCAGCTTTTAAA+TGG | - | chr4.1:17118150-17118169 | MS.gene08362:intron | 30.0% |
| ! | TCATTAGACATTTTGGACTA+GGG | - | chr4.1:17118252-17118271 | MS.gene08362:intron | 30.0% |
| ! | TTCATTAGACATTTTGGACT+AGG | - | chr4.1:17118251-17118270 | MS.gene08362:intron | 30.0% |
| !! | ATTTTAGGAAGAAAAGGAGA+GGG | - | chr4.1:17117668-17117687 | MS.gene08362:CDS | 30.0% |
| !! | TATTTTAGGAAGAAAAGGAG+AGG | - | chr4.1:17117667-17117686 | MS.gene08362:CDS | 30.0% |
| ACACTAATGACTGAAGATTC+AGG | + | chr4.1:17117864-17117883 | None:intergenic | 35.0% | |
| ACATTTAACAACTGCTACCA+AGG | + | chr4.1:17118358-17118377 | None:intergenic | 35.0% | |
| ACCAATTTGGAAATTTCAGG+TGG | - | chr4.1:17118085-17118104 | MS.gene08362:intron | 35.0% | |
| ACCACCTGAAATTTCCAAAT+TGG | + | chr4.1:17118089-17118108 | None:intergenic | 35.0% | |
| ATGAAAGGATGCATGAAAAG+AGG | - | chr4.1:17118575-17118594 | MS.gene08362:CDS | 35.0% | |
| ATTATATGAACACGGCTACA+AGG | + | chr4.1:17118037-17118056 | None:intergenic | 35.0% | |
| CAGTCATAGAGTTAGTTAAG+AGG | + | chr4.1:17117481-17117500 | None:intergenic | 35.0% | |
| CCATTGATCAAAAGGGTAAT+TGG | - | chr4.1:17117511-17117530 | MS.gene08362:CDS | 35.0% | |
| TACACAGAACAGTATCATCA+TGG | - | chr4.1:17117722-17117741 | MS.gene08362:intron | 35.0% | |
| ! | AGCTGCTGCTATATATGTTT+TGG | + | chr4.1:17117602-17117621 | None:intergenic | 35.0% |
| ! | CATGACTTTTACAGGATGAA+AGG | - | chr4.1:17118560-17118579 | MS.gene08362:CDS | 35.0% |
| ! | CCAATTACCCTTTTGATCAA+TGG | + | chr4.1:17117514-17117533 | None:intergenic | 35.0% |
| !! | CATTAGACATTTTGGACTAG+GGG | - | chr4.1:17118253-17118272 | MS.gene08362:intron | 35.0% |
| ACTGTTCTGTGTAGTTACAC+TGG | + | chr4.1:17117715-17117734 | None:intergenic | 40.0% | |
| AGATGTGGCATTCTCATCAA+TGG | + | chr4.1:17118745-17118764 | None:intergenic | 40.0% | |
| ATGACTGAAGATTCAGGCAT+TGG | + | chr4.1:17117858-17117877 | None:intergenic | 40.0% | |
| CAACAATTTACCGGTCATTC+TGG | - | chr4.1:17118403-17118422 | MS.gene08362:CDS | 40.0% | |
| CTAGAAAGAGAAGCTGCAAA+GGG | + | chr4.1:17118281-17118300 | None:intergenic | 40.0% | |
| GGGACCAATTTGGAAATTTC+AGG | - | chr4.1:17118082-17118101 | MS.gene08362:intron | 40.0% | |
| GTCACTAAACACTAGGCTAA+GGG | - | chr4.1:17118062-17118081 | MS.gene08362:intron | 40.0% | |
| TCAGTAGTTTCCAGAATGAC+CGG | + | chr4.1:17118416-17118435 | None:intergenic | 40.0% | |
| TGTCACTAAACACTAGGCTA+AGG | - | chr4.1:17118061-17118080 | MS.gene08362:intron | 40.0% | |
| TTTCCCAGTTGAACATCCTA+TGG | - | chr4.1:17117800-17117819 | MS.gene08362:intron | 40.0% | |
| ! | ACAGAAAGCATAAGGAAGAG+AGG | - | chr4.1:17118601-17118620 | MS.gene08362:intron | 40.0% |
| ! | AGAGGAACACAGAAAGCATA+AGG | - | chr4.1:17118593-17118612 | MS.gene08362:intron | 40.0% |
| ! | CTGACAAGCATGACTTTTAC+AGG | - | chr4.1:17118552-17118571 | MS.gene08362:CDS | 40.0% |
| ! | TATCAACCCTCTGATGATGA+TGG | + | chr4.1:17117767-17117786 | None:intergenic | 40.0% |
| !! | ATATTCTGGGATTGTAGAGG+AGG | + | chr4.1:17118769-17118788 | None:intergenic | 40.0% |
| !! | GCAGTTCATTCTGAAGTTCA+GGG | - | chr4.1:17118517-17118536 | MS.gene08362:CDS | 40.0% |
| !! | GTGATATTCTGGGATTGTAG+AGG | + | chr4.1:17118772-17118791 | None:intergenic | 40.0% |
| !! | TATTCTGGGATTGTAGAGGA+GGG | + | chr4.1:17118768-17118787 | None:intergenic | 40.0% |
| !! | TGCAGTTCATTCTGAAGTTC+AGG | - | chr4.1:17118516-17118535 | MS.gene08362:CDS | 40.0% |
| ACTAGGCTAAGGGACCAATT+TGG | - | chr4.1:17118072-17118091 | MS.gene08362:intron | 45.0% | |
| ATGGCAAAGCATGCTTTGCA+TGG | - | chr4.1:17117926-17117945 | MS.gene08362:intron | 45.0% | |
| GCTAGAAAGAGAAGCTGCAA+AGG | + | chr4.1:17118282-17118301 | None:intergenic | 45.0% | |
| GCTCCATAGGATGTTCAACT+GGG | + | chr4.1:17117806-17117825 | None:intergenic | 45.0% | |
| TCCTGATGATCCATGAGTGA+GGG | + | chr4.1:17118707-17118726 | None:intergenic | 45.0% | |
| TTTCCAAGTGCTTCAGCAGT+TGG | - | chr4.1:17118795-17118814 | MS.gene08362:intron | 45.0% | |
| !! | TCAGGCATTGGACATTTCAC+CGG | + | chr4.1:17117846-17117865 | None:intergenic | 45.0% |
| ACCACCACCATCATCATCAG+AGG | - | chr4.1:17117757-17117776 | MS.gene08362:intron | 50.0% | |
| ACCCTCACTCATGGATCATC+AGG | - | chr4.1:17118703-17118722 | MS.gene08362:intron | 50.0% | |
| AGGAAGAGAGGTGAGATGTC+TGG | - | chr4.1:17118613-17118632 | MS.gene08362:intron | 50.0% | |
| CCACCACCATCATCATCAGA+GGG | - | chr4.1:17117758-17117777 | MS.gene08362:intron | 50.0% | |
| CTCCTGATGATCCATGAGTG+AGG | + | chr4.1:17118708-17118727 | None:intergenic | 50.0% | |
| GATTGTAGAGGAGGGAGATG+TGG | + | chr4.1:17118760-17118779 | None:intergenic | 50.0% | |
| GGAAGAGAGGTGAGATGTCT+GGG | - | chr4.1:17118614-17118633 | MS.gene08362:intron | 50.0% | |
| GGCTCCATAGGATGTTCAAC+TGG | + | chr4.1:17117807-17117826 | None:intergenic | 50.0% | |
| TGAACATCCTATGGAGCCAC+CGG | - | chr4.1:17117809-17117828 | MS.gene08362:intron | 50.0% | |
| TGGACAAAGAACAGCTGGCA+TGG | - | chr4.1:17118639-17118658 | MS.gene08362:intron | 50.0% | |
| TTCACCGGACGATCTTCATC+CGG | + | chr4.1:17117831-17117850 | None:intergenic | 50.0% | |
| ! | TGTCCAACTGCTGAAGCACT+TGG | + | chr4.1:17118801-17118820 | None:intergenic | 50.0% |
| !! | CAACCCTCTGATGATGATGG+TGG | + | chr4.1:17117764-17117783 | None:intergenic | 50.0% |
| AGAGGTGAGATGTCTGGGTC+TGG | - | chr4.1:17118619-17118638 | MS.gene08362:intron | 55.0% | |
| GGGTCTGGACAAAGAACAGC+TGG | - | chr4.1:17118634-17118653 | MS.gene08362:intron | 55.0% | |
| TCTTCATCCGGTGGCTCCAT+AGG | + | chr4.1:17117819-17117838 | None:intergenic | 55.0% | |
| !! | CCCTCTGATGATGATGGTGG+TGG | + | chr4.1:17117761-17117780 | None:intergenic | 55.0% |
| ACCGGACGATCTTCATCCGG+TGG | + | chr4.1:17117828-17117847 | None:intergenic | 60.0% | |
| CGGCATCACACCCTCACTCA+TGG | - | chr4.1:17118694-17118713 | MS.gene08362:intron | 60.0% | |
| CTTTCGCACTCCTCGAGCTG+GGG | + | chr4.1:17118674-17118693 | None:intergenic | 60.0% | |
| GCCACCGGATGAAGATCGTC+CGG | - | chr4.1:17117824-17117843 | MS.gene08362:intron | 60.0% | |
| GCTTTCGCACTCCTCGAGCT+GGG | + | chr4.1:17118675-17118694 | None:intergenic | 60.0% | |
| ! | CAGCTCGAGGAGTGCGAAAG+CGG | - | chr4.1:17118674-17118693 | MS.gene08362:intron | 60.0% |
| CGCTTTCGCACTCCTCGAGC+TGG | + | chr4.1:17118676-17118695 | None:intergenic | 65.0% | |
| GACACAGATGCCCCAGCTCG+AGG | - | chr4.1:17118661-17118680 | MS.gene08362:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 17117445 | 17118834 | 17117445 | ID=MS.gene08362 |
| chr4.1 | mRNA | 17117445 | 17118834 | 17117445 | ID=MS.gene08362.t1;Parent=MS.gene08362 |
| chr4.1 | exon | 17118808 | 17118834 | 17118808 | ID=MS.gene08362.t1.exon1;Parent=MS.gene08362.t1 |
| chr4.1 | CDS | 17118808 | 17118834 | 17118808 | ID=cds.MS.gene08362.t1;Parent=MS.gene08362.t1 |
| chr4.1 | exon | 17118398 | 17118604 | 17118398 | ID=MS.gene08362.t1.exon2;Parent=MS.gene08362.t1 |
| chr4.1 | CDS | 17118398 | 17118604 | 17118398 | ID=cds.MS.gene08362.t1;Parent=MS.gene08362.t1 |
| chr4.1 | exon | 17117445 | 17117705 | 17117445 | ID=MS.gene08362.t1.exon3;Parent=MS.gene08362.t1 |
| chr4.1 | CDS | 17117445 | 17117705 | 17117445 | ID=cds.MS.gene08362.t1;Parent=MS.gene08362.t1 |
| Gene Sequence |
| Protein sequence |
