AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08393

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08393.t1 AT4G02620 87.302 126 16 0 1 126 1 126 4.46e-80 231

Find 28 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCATAGTTGGGTTTCTTT+TGG 0.252743 4.1:-17481800 MS.gene08393:CDS
CTCATGATTCAGTTCTTTCA+CGG 0.364488 4.1:-17480307 MS.gene08393:CDS
TTTCCACAGGATACCATAGT+TGG 0.389583 4.1:-17481811 MS.gene08393:intron
GAAGGGATTTCAAGAATTGC+AGG 0.394210 4.1:+17480353 None:intergenic
ATTTCAAGAATTGCAGGAAC+TGG 0.410123 4.1:+17480359 None:intergenic
GAATCATGAGCAGGATCATA+AGG 0.423283 4.1:+17480320 None:intergenic
AGCCAAAAGAAACCCAACTA+TGG 0.431491 4.1:+17481798 None:intergenic
ACTTACTTGAATCCACAATA+AGG 0.438291 4.1:+17481737 None:intergenic
TGCAGGTTGCAAATATGATA+AGG 0.450588 4.1:-17480406 MS.gene08393:intron
GATGAATTGTTGGCAGGAAT+CGG 0.453548 4.1:+17483075 None:intergenic
GGCAATAAGTGATGAATTGT+TGG 0.454916 4.1:+17483065 None:intergenic
GCTTACCTCGTCAGCAATCA+TGG 0.470639 4.1:+17483044 None:intergenic
GAAGACAAATTACCTTATTG+TGG 0.485357 4.1:-17481749 MS.gene08393:CDS
TTCCACAGGATACCATAGTT+GGG 0.495606 4.1:-17481810 MS.gene08393:intron
TTCAATGCTGAATCAGTGGC+AGG 0.511114 4.1:-17480272 MS.gene08393:CDS
TCATGATTCAGTTCTTTCAC+GGG 0.514197 4.1:-17480306 MS.gene08393:CDS
GGCAGGAATCGGAACTCTGT+TGG 0.544048 4.1:+17483086 None:intergenic
ATAAGTGATGAATTGTTGGC+AGG 0.546049 4.1:+17483069 None:intergenic
AATCATGAGCAGGATCATAA+GGG 0.560484 4.1:+17480321 None:intergenic
GAAAGAACTGAATCATGAGC+AGG 0.572900 4.1:+17480311 None:intergenic
TATTGCCATGATTGCTGACG+AGG 0.587291 4.1:-17483049 MS.gene08393:intron
TCTCTTCAATGCTGAATCAG+TGG 0.629677 4.1:-17480276 MS.gene08393:CDS
AACGACTGTCAAACAAATCG+AGG 0.646091 4.1:-17481108 MS.gene08393:CDS
CAAAGAATTCTCAACACGGG+AGG 0.652854 4.1:-17481078 MS.gene08393:CDS
ATTCAAAGAATTCTCAACAC+GGG 0.675761 4.1:-17481081 MS.gene08393:CDS
CATTCAAAGAATTCTCAACA+CGG 0.678390 4.1:-17481082 MS.gene08393:CDS
CATGAGCAGGATCATAAGGG+TGG 0.700765 4.1:+17480324 None:intergenic
TGGGAAACGTTGACATACGT+CGG 0.705364 4.1:-17481771 MS.gene08393:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAACTAAAATGGAAT+TGG + chr4.1:17481443-17481462 None:intergenic 15.0%
!! AAAAAAACTAAAATGGAATT+GGG + chr4.1:17481442-17481461 None:intergenic 15.0%
!! ACTAGTTATTCAAATTAATA+GGG + chr4.1:17481361-17481380 None:intergenic 15.0%
!! GAAAACTCAATTAATAAAAA+AGG + chr4.1:17480758-17480777 None:intergenic 15.0%
!! TATGAAATGTATTAAAGATA+AGG + chr4.1:17481910-17481929 None:intergenic 15.0%
!! TTACTTACTATTATTTATGA+GGG - chr4.1:17481648-17481667 MS.gene08393:intron 15.0%
!! TTTACTTACTATTATTTATG+AGG - chr4.1:17481647-17481666 MS.gene08393:intron 15.0%
!! AAATACCAGCTTATATAATA+TGG + chr4.1:17480976-17480995 None:intergenic 20.0%
!! AACATGTGTTAAACTATAAT+AGG + chr4.1:17482479-17482498 None:intergenic 20.0%
!! AATTTGTAAGTCATATTTCA+TGG - chr4.1:17482318-17482337 MS.gene08393:intron 20.0%
!! ATGAAATATGACTTACAAAT+TGG + chr4.1:17482319-17482338 None:intergenic 20.0%
!! ATTAACAGGAAAAAATTGTA+TGG + chr4.1:17481690-17481709 None:intergenic 20.0%
!! CACTAGTTATTCAAATTAAT+AGG + chr4.1:17481362-17481381 None:intergenic 20.0%
!! CTAGTTATTCAAATTAATAG+GGG + chr4.1:17481360-17481379 None:intergenic 20.0%
!! TAACAGGATTTAAATGAAAT+AGG - chr4.1:17480801-17480820 MS.gene08393:intron 20.0%
!! TTAACAGGAAAAAATTGTAT+GGG + chr4.1:17481689-17481708 None:intergenic 20.0%
!! TTAATAAAAAAGGAAATCAG+TGG + chr4.1:17480748-17480767 None:intergenic 20.0%
!!! AAAATGTTGACTAGTTTATA+TGG - chr4.1:17482727-17482746 MS.gene08393:intron 20.0%
!!! AACACATGTACTATTTTTAT+TGG + chr4.1:17481778-17481797 None:intergenic 20.0%
!!! AATTCAATTTTATTTTCCAC+AGG - chr4.1:17481525-17481544 MS.gene08393:intron 20.0%
!!! ATAAGAAAGTATATACAACA+TGG + chr4.1:17480337-17480356 None:intergenic 20.0%
!!! TAAGAAAGTATATACAACAT+GGG + chr4.1:17480336-17480355 None:intergenic 20.0%
!!! TGTTTTATTTCCTTATTTCA+GGG + chr4.1:17481128-17481147 None:intergenic 20.0%
!!! TTAAAGATCTATTTTGCTAT+TGG - chr4.1:17480485-17480504 MS.gene08393:intron 20.0%
!!! TTCAAAACAAATTGATTTGA+CGG - chr4.1:17482181-17482200 MS.gene08393:intron 20.0%
!!! TTTTTGCTAGAATCTATTTA+AGG + chr4.1:17480780-17480799 None:intergenic 20.0%
! AAAATCAGATATAGTTCAGT+CGG - chr4.1:17481314-17481333 MS.gene08393:intron 25.0%
! AAATGAAATAGGAAATTGTG+AGG - chr4.1:17480812-17480831 MS.gene08393:intron 25.0%
! AATCAGAAAATTAGGTGAAA+AGG + chr4.1:17481744-17481763 None:intergenic 25.0%
! AATCAGATGATATGATAACT+AGG - chr4.1:17480385-17480404 MS.gene08393:CDS 25.0%
! AGATTCTAGCAAAAATTAAC+AGG - chr4.1:17480785-17480804 MS.gene08393:intron 25.0%
! ATACTCCTATTTCATTAACA+AGG + chr4.1:17481017-17481036 None:intergenic 25.0%
! CTCTGATAAAATTGAAGAAA+GGG - chr4.1:17480866-17480885 MS.gene08393:intron 25.0%
! GTGAGATTCAATCATAAAAT+TGG - chr4.1:17482646-17482665 MS.gene08393:intron 25.0%
! GTGTTAAACTATAATAGGTA+AGG + chr4.1:17482474-17482493 None:intergenic 25.0%
! TAAATAACCCAAGATTGTAA+CGG + chr4.1:17482901-17482920 None:intergenic 25.0%
! TAACAGGAAAAAATTGTATG+GGG + chr4.1:17481688-17481707 None:intergenic 25.0%
! TACTTCCATATTATATAAGC+TGG - chr4.1:17480968-17480987 MS.gene08393:intron 25.0%
! TAGCCAATTACTAGTATAAA+CGG - chr4.1:17481100-17481119 MS.gene08393:CDS 25.0%
! TATAATTTCTTTGACTATGC+AGG - chr4.1:17482926-17482945 MS.gene08393:intron 25.0%
! TCAGTTATTAGTTGGATATT+TGG - chr4.1:17482772-17482791 MS.gene08393:intron 25.0%
! TCTCTGATAAAATTGAAGAA+AGG - chr4.1:17480865-17480884 MS.gene08393:intron 25.0%
! TGTATTAAAGATAAGGTTTG+TGG + chr4.1:17481903-17481922 None:intergenic 25.0%
! TTTATGCAAAGGTTATTAGT+AGG - chr4.1:17481986-17482005 MS.gene08393:intron 25.0%
!! AAACCGTAGAATACTTATTT+TGG - chr4.1:17481949-17481968 MS.gene08393:intron 25.0%
!! AAATTTAGTTTCTTTGGATG+AGG + chr4.1:17480842-17480861 None:intergenic 25.0%
!! ACTATTATTGTACATTTTCC+TGG - chr4.1:17480552-17480571 MS.gene08393:intron 25.0%
!! AGAGACAAATTTAGTTTCTT+TGG + chr4.1:17480848-17480867 None:intergenic 25.0%
!! AGGATAAGTAAAAAGTCTTT+TGG + chr4.1:17482454-17482473 None:intergenic 25.0%
!! ATTTTATGATTGAATCTCAC+TGG + chr4.1:17482646-17482665 None:intergenic 25.0%
!! TAGATTTGACTTAGAGTAAT+TGG + chr4.1:17481082-17481101 None:intergenic 25.0%
!! TATTAGCTAAGATGATTTTG+CGG + chr4.1:17480434-17480453 None:intergenic 25.0%
!! TGCAAATATGATAAGGTTTT+TGG - chr4.1:17482950-17482969 MS.gene08393:intron 25.0%
!!! AACCGTAGAATACTTATTTT+GGG - chr4.1:17481950-17481969 MS.gene08393:intron 25.0%
!!! GTGTTTTATTTCCTTATTTC+AGG + chr4.1:17481129-17481148 None:intergenic 25.0%
!!! TTTTAATTATCTACTGCATC+AGG - chr4.1:17482545-17482564 MS.gene08393:intron 25.0%
AAAATCCATACAATGACAGT+TGG - chr4.1:17481165-17481184 MS.gene08393:intron 30.0%
AACAGGAAAAAATTGTATGG+GGG + chr4.1:17481687-17481706 None:intergenic 30.0%
AATGAATAATGCTGGATAAC+TGG - chr4.1:17482589-17482608 MS.gene08393:intron 30.0%
ACTTACTTGAATCCACAATA+AGG + chr4.1:17481615-17481634 None:intergenic 30.0%
AGAAAACCTTCTTGCTTATT+TGG - chr4.1:17482875-17482894 MS.gene08393:intron 30.0%
AGTTTAACACATGTTCTGTT+TGG - chr4.1:17482484-17482503 MS.gene08393:intron 30.0%
ATGAGGTTAATGAATAATGC+TGG - chr4.1:17482581-17482600 MS.gene08393:intron 30.0%
ATTCAAAGAATTCTCAACAC+GGG - chr4.1:17482268-17482287 MS.gene08393:intron 30.0%
CATTCAAAGAATTCTCAACA+CGG - chr4.1:17482267-17482286 MS.gene08393:intron 30.0%
CATTTCTACACATCCTATAT+AGG + chr4.1:17481871-17481890 None:intergenic 30.0%
GAAGACAAATTACCTTATTG+TGG - chr4.1:17481600-17481619 MS.gene08393:intron 30.0%
GGTAATCAACACTATTGTTA+GGG - chr4.1:17482505-17482524 MS.gene08393:intron 30.0%
GTTCCGTTTATACTAGTAAT+TGG + chr4.1:17481106-17481125 None:intergenic 30.0%
TAAAAACTATTTCATGCACC+CGG - chr4.1:17482843-17482862 MS.gene08393:intron 30.0%
TAGAAAATAAGTGGATGGAT+AGG - chr4.1:17480647-17480666 MS.gene08393:intron 30.0%
TCTACCACAATCAGAAAATT+AGG + chr4.1:17481752-17481771 None:intergenic 30.0%
TCTTGAAAGGATCAAATTCA+GGG - chr4.1:17480622-17480641 MS.gene08393:intron 30.0%
TGACTTCTTAAGTCATCTTA+TGG + chr4.1:17480695-17480714 None:intergenic 30.0%
TGCCCAAAATAAGTATTCTA+CGG + chr4.1:17481955-17481974 None:intergenic 30.0%
TGGTAATCAACACTATTGTT+AGG - chr4.1:17482504-17482523 MS.gene08393:intron 30.0%
TTAATTGGTACTTGAACCTT+GGG - chr4.1:17482025-17482044 MS.gene08393:intron 30.0%
TTCAGGGTTTAGAAAATAAG+TGG - chr4.1:17480638-17480657 MS.gene08393:intron 30.0%
! AAAAGGAAATCAGTGGTTTT+AGG + chr4.1:17480741-17480760 None:intergenic 30.0%
! AACTGTCATTGTATGGATTT+TGG + chr4.1:17481166-17481185 None:intergenic 30.0%
! AAGGTTTTCTAAATAGGAAC+CGG + chr4.1:17482865-17482884 None:intergenic 30.0%
! AAGTATTCTACGGTTTTTCT+GGG + chr4.1:17481945-17481964 None:intergenic 30.0%
! AGCAAGAAGGTTTTCTAAAT+AGG + chr4.1:17482871-17482890 None:intergenic 30.0%
! ATAGTTTTTAAATCCCAGCT+AGG + chr4.1:17482833-17482852 None:intergenic 30.0%
! CATTAACCTCATGTAAGTTT+TGG + chr4.1:17482573-17482592 None:intergenic 30.0%
! GATTGCCTTGTTAATGAAAT+AGG - chr4.1:17481009-17481028 MS.gene08393:intron 30.0%
! TAAGTATTCTACGGTTTTTC+TGG + chr4.1:17481946-17481965 None:intergenic 30.0%
! TTCACCTAATTTTCTGATTG+TGG - chr4.1:17481745-17481764 MS.gene08393:CDS 30.0%
!! AACAGCCTGAATCATTTTTA+TGG + chr4.1:17482344-17482363 None:intergenic 30.0%
!! TCAAGTTCTGAAAATATTCC+TGG - chr4.1:17482142-17482161 MS.gene08393:intron 30.0%
!! TGACTAGTTTATATGGTCAT+TGG - chr4.1:17482734-17482753 MS.gene08393:intron 30.0%
!!! AGAATACTTATTTTGGGCAT+GGG - chr4.1:17481956-17481975 MS.gene08393:intron 30.0%
!!! ATTGTATGGATTTTGGCTTT+GGG + chr4.1:17481159-17481178 None:intergenic 30.0%
!!! TAGAATACTTATTTTGGGCA+TGG - chr4.1:17481955-17481974 MS.gene08393:intron 30.0%
!!! TGGGCTATTTTTTTATGCAA+AGG - chr4.1:17481975-17481994 MS.gene08393:intron 30.0%
AAACTGAGCAAGAAATTACC+AGG + chr4.1:17482163-17482182 None:intergenic 35.0%
AACATGATGCACAGATTAAC+AGG + chr4.1:17481704-17481723 None:intergenic 35.0%
AATCATGAGCAGGATCATAA+GGG + chr4.1:17483031-17483050 None:intergenic 35.0%
ACCAATTAACCTCAGTTTCA+GGG + chr4.1:17482014-17482033 None:intergenic 35.0%
ACTAGGATGCATGATTGAAT+AGG - chr4.1:17480402-17480421 MS.gene08393:CDS 35.0%
AGAAAATTAGGTGAAAAGGC+AGG + chr4.1:17481740-17481759 None:intergenic 35.0%
AGACTTACTGTTGTATATCG+TGG - chr4.1:17481814-17481833 MS.gene08393:intron 35.0%
ATAAACGGAACCCTGAAATA+AGG - chr4.1:17481115-17481134 MS.gene08393:intron 35.0%
ATAAGTGATGAATTGTTGGC+AGG + chr4.1:17480283-17480302 None:intergenic 35.0%
ATTGTTAGGGAAAATCTGGA+GGG - chr4.1:17482518-17482537 MS.gene08393:intron 35.0%
ATTTCAAGAATTGCAGGAAC+TGG + chr4.1:17482993-17483012 None:intergenic 35.0%
CACTATTGTTAGGGAAAATC+TGG - chr4.1:17482514-17482533 MS.gene08393:intron 35.0%
CATGGCCATAAAAATGATTC+AGG - chr4.1:17482336-17482355 MS.gene08393:intron 35.0%
CTAGGATGCATGATTGAATA+GGG - chr4.1:17480403-17480422 MS.gene08393:CDS 35.0%
CTCATGATTCAGTTCTTTCA+CGG - chr4.1:17483042-17483061 MS.gene08393:intron 35.0%
CTCTTGAAAGGATCAAATTC+AGG - chr4.1:17480621-17480640 MS.gene08393:intron 35.0%
CTGTTGGATCAGTTATTAGT+TGG - chr4.1:17482764-17482783 MS.gene08393:intron 35.0%
GAAAATTAGGTGAAAAGGCA+GGG + chr4.1:17481739-17481758 None:intergenic 35.0%
GAAAGGGATTTAGAGATCAT+AGG - chr4.1:17480882-17480901 MS.gene08393:intron 35.0%
GGCAATAAGTGATGAATTGT+TGG + chr4.1:17480287-17480306 None:intergenic 35.0%
GGGTTTAATAGGTTAGGTTT+AGG - chr4.1:17480596-17480615 MS.gene08393:intron 35.0%
GGGTTTAGAAAATAAGTGGA+TGG - chr4.1:17480642-17480661 MS.gene08393:intron 35.0%
GTTAATTGGTACTTGAACCT+TGG - chr4.1:17482024-17482043 MS.gene08393:intron 35.0%
TACCAATTAACCTCAGTTTC+AGG + chr4.1:17482015-17482034 None:intergenic 35.0%
TATTGTTAGGGAAAATCTGG+AGG - chr4.1:17482517-17482536 MS.gene08393:intron 35.0%
TATTTGGCCGTTACAATCTT+GGG - chr4.1:17482891-17482910 MS.gene08393:intron 35.0%
TCATGATTCAGTTCTTTCAC+GGG - chr4.1:17483043-17483062 MS.gene08393:intron 35.0%
TGCAGGTTGCAAATATGATA+AGG - chr4.1:17482943-17482962 MS.gene08393:intron 35.0%
TTATTTGGCCGTTACAATCT+TGG - chr4.1:17482890-17482909 MS.gene08393:intron 35.0%
TTGTCGACATTTAAACCCAA+AGG + chr4.1:17482691-17482710 None:intergenic 35.0%
! ACTTTCAGTTTTCAGCCATA+GGG + chr4.1:17481258-17481277 None:intergenic 35.0%
! AGGTTTTCTAAATAGGAACC+GGG + chr4.1:17482864-17482883 None:intergenic 35.0%
! TACCATAGTTGGGTTTCTTT+TGG - chr4.1:17481549-17481568 MS.gene08393:intron 35.0%
! TACTTTCAGTTTTCAGCCAT+AGG + chr4.1:17481259-17481278 None:intergenic 35.0%
! TAGGTTTAGGTTCTCTTGAA+AGG - chr4.1:17480609-17480628 MS.gene08393:intron 35.0%
! TATTGTACATTTTCCTGGTG+TGG - chr4.1:17480557-17480576 MS.gene08393:intron 35.0%
!! TTTTCTTAAGAAGTGTGCAG+TGG + chr4.1:17480529-17480548 None:intergenic 35.0%
!!! CATTGTATGGATTTTGGCTT+TGG + chr4.1:17481160-17481179 None:intergenic 35.0%
!!! TTGTATGGATTTTGGCTTTG+GGG + chr4.1:17481158-17481177 None:intergenic 35.0%
AACGACTGTCAAACAAATCG+AGG - chr4.1:17482241-17482260 MS.gene08393:intron 40.0%
AAGTAGGAACTAACAGTAGC+AGG + chr4.1:17482401-17482420 None:intergenic 40.0%
ACACTCCAACTGTCATTGTA+TGG + chr4.1:17481173-17481192 None:intergenic 40.0%
ACCGCACTAAAATATCCCTA+TGG - chr4.1:17481240-17481259 MS.gene08393:intron 40.0%
ACGTAATAAGCTGATGTCTC+AGG + chr4.1:17482431-17482450 None:intergenic 40.0%
ACTGCTTGGTACAAATCACA+GGG + chr4.1:17481045-17481064 None:intergenic 40.0%
AGCCAAAAGAAACCCAACTA+TGG + chr4.1:17481554-17481573 None:intergenic 40.0%
AGTAATCCAAGTACTACCCA+AGG + chr4.1:17482044-17482063 None:intergenic 40.0%
ATCTGTGCATCATGTTCATG+TGG - chr4.1:17481708-17481727 MS.gene08393:intron 40.0%
CAATGGCTGAATGAGCTAAA+AGG + chr4.1:17482804-17482823 None:intergenic 40.0%
CAGGTTCCAAAACTTACATG+AGG - chr4.1:17482564-17482583 MS.gene08393:intron 40.0%
CGACATTTAAACCCAAAGGA+TGG + chr4.1:17482687-17482706 None:intergenic 40.0%
CGGCAATTGAACATTACAAC+TGG - chr4.1:17481334-17481353 MS.gene08393:intron 40.0%
CTGTTAGTTCCTACTTCCTA+CGG - chr4.1:17482405-17482424 MS.gene08393:intron 40.0%
GAAAGAACTGAATCATGAGC+AGG + chr4.1:17483041-17483060 None:intergenic 40.0%
GAAGGGATTTCAAGAATTGC+AGG + chr4.1:17482999-17483018 None:intergenic 40.0%
GAATCATGAGCAGGATCATA+AGG + chr4.1:17483032-17483051 None:intergenic 40.0%
GATGAATTGTTGGCAGGAAT+CGG + chr4.1:17480277-17480296 None:intergenic 40.0%
GATTGCATCACACATAGTCA+AGG - chr4.1:17480935-17480954 MS.gene08393:intron 40.0%
GGGATATGTCTCTCCTATAT+AGG - chr4.1:17481855-17481874 MS.gene08393:intron 40.0%
TAACGGCCAAATAAGCAAGA+AGG + chr4.1:17482884-17482903 None:intergenic 40.0%
TACAATGACAGTTGGAGTGT+TGG - chr4.1:17481173-17481192 MS.gene08393:intron 40.0%
TAGAAGGCTTGGGGTTTAAT+AGG - chr4.1:17480585-17480604 MS.gene08393:intron 40.0%
TATCCCCATGCTTATTTACG+AGG + chr4.1:17481841-17481860 None:intergenic 40.0%
TCTCTTCAATGCTGAATCAG+TGG - chr4.1:17483073-17483092 MS.gene08393:CDS 40.0%
TCTTTCGATAGCCATCCTTT+GGG - chr4.1:17482673-17482692 MS.gene08393:intron 40.0%
TGGACCTCGTAAATAAGCAT+GGG - chr4.1:17481834-17481853 MS.gene08393:intron 40.0%
TTCCACAGGATACCATAGTT+GGG - chr4.1:17481539-17481558 MS.gene08393:intron 40.0%
TTCTTTCGATAGCCATCCTT+TGG - chr4.1:17482672-17482691 MS.gene08393:intron 40.0%
TTTCCACAGGATACCATAGT+TGG - chr4.1:17481538-17481557 MS.gene08393:intron 40.0%
! ATAGTTGGGTTTCTTTTGGC+TGG - chr4.1:17481553-17481572 MS.gene08393:intron 40.0%
! CATAAGGGTGGTCTTTTGAA+GGG + chr4.1:17483016-17483035 None:intergenic 40.0%
! TCATAAGGGTGGTCTTTTGA+AGG + chr4.1:17483017-17483036 None:intergenic 40.0%
!!! GCCATAGGGATATTTTAGTG+CGG + chr4.1:17481244-17481263 None:intergenic 40.0%
!!! TGTATGGATTTTGGCTTTGG+GGG + chr4.1:17481157-17481176 None:intergenic 40.0%
AACCCAACTATGGTATCCTG+TGG + chr4.1:17481544-17481563 None:intergenic 45.0%
AGCCATTGTGATATCCTAGC+TGG - chr4.1:17482816-17482835 MS.gene08393:intron 45.0%
ATGGATAGGTTCTCGAGGAA+GGG - chr4.1:17480661-17480680 MS.gene08393:intron 45.0%
CAAAGAATTCTCAACACGGG+AGG - chr4.1:17482271-17482290 MS.gene08393:intron 45.0%
CTTGAACCTTGGGTAGTACT+TGG - chr4.1:17482035-17482054 MS.gene08393:intron 45.0%
GACTGCTTGGTACAAATCAC+AGG + chr4.1:17481046-17481065 None:intergenic 45.0%
GCCATTGTGATATCCTAGCT+GGG - chr4.1:17482817-17482836 MS.gene08393:intron 45.0%
GCCCTGAAACTGAGGTTAAT+TGG - chr4.1:17482010-17482029 MS.gene08393:intron 45.0%
GGAACTAACAGTAGCAGGTA+TGG + chr4.1:17482396-17482415 None:intergenic 45.0%
GGACCTCGTAAATAAGCATG+GGG - chr4.1:17481835-17481854 MS.gene08393:intron 45.0%
GGCTGAATGAGCTAAAAGGA+CGG + chr4.1:17482800-17482819 None:intergenic 45.0%
GGCTTGGGGTTTAATAGGTT+AGG - chr4.1:17480590-17480609 MS.gene08393:intron 45.0%
GTGGACCTCGTAAATAAGCA+TGG - chr4.1:17481833-17481852 MS.gene08393:intron 45.0%
TATTGCCATGATTGCTGACG+AGG - chr4.1:17480300-17480319 MS.gene08393:CDS 45.0%
TCCCAGCTAGGATATCACAA+TGG + chr4.1:17482821-17482840 None:intergenic 45.0%
TCTCACTGGTACTGTCACAT+TGG + chr4.1:17482632-17482651 None:intergenic 45.0%
TGCATCACACATAGTCAAGG+AGG - chr4.1:17480938-17480957 MS.gene08393:intron 45.0%
TGGGAAACGTTGACATACGT+CGG - chr4.1:17481578-17481597 MS.gene08393:intron 45.0%
TTCAATGCTGAATCAGTGGC+AGG - chr4.1:17483077-17483096 MS.gene08393:CDS 45.0%
! TGTGGAGTACTAGAAGGCTT+GGG - chr4.1:17480575-17480594 MS.gene08393:intron 45.0%
!! GGTCATTGGTTTCTCACTGT+TGG - chr4.1:17482748-17482767 MS.gene08393:intron 45.0%
!!! TATTTTAGTGCGGTGCTGCT+AGG + chr4.1:17481234-17481253 None:intergenic 45.0%
!! AAAAAAAAAAAAAACTAAAA+TGG + chr4.1:17481449-17481468 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTGTAAT+TGG - chr4.1:17481460-17481479 MS.gene08393:intron 5.0%
ACCTGCAGTTTCAGACTGCT+TGG + chr4.1:17481059-17481078 None:intergenic 50.0%
AGTGGATGGATAGGTTCTCG+AGG - chr4.1:17480656-17480675 MS.gene08393:intron 50.0%
CATGAGCAGGATCATAAGGG+TGG + chr4.1:17483028-17483047 None:intergenic 50.0%
GATGGATAGGTTCTCGAGGA+AGG - chr4.1:17480660-17480679 MS.gene08393:intron 50.0%
GCTTACCTCGTCAGCAATCA+TGG + chr4.1:17480308-17480327 None:intergenic 50.0%
TAGTAGGTGCCCTGAAACTG+AGG - chr4.1:17482002-17482021 MS.gene08393:intron 50.0%
! ACCAAGCAGTCTGAAACTGC+AGG - chr4.1:17481055-17481074 MS.gene08393:CDS 50.0%
! GTGGAGTACTAGAAGGCTTG+GGG - chr4.1:17480576-17480595 MS.gene08393:intron 50.0%
! GTGTGGAGTACTAGAAGGCT+TGG - chr4.1:17480574-17480593 MS.gene08393:intron 50.0%
! TCCTGGTGTGGAGTACTAGA+AGG - chr4.1:17480569-17480588 MS.gene08393:intron 50.0%
!! GGGTTTCTTTTGGCTGGAGT+GGG - chr4.1:17481559-17481578 MS.gene08393:intron 50.0%
!! TGGGTTTCTTTTGGCTGGAG+TGG - chr4.1:17481558-17481577 MS.gene08393:intron 50.0%
AAGCTGATGTCTCAGGCCGT+AGG + chr4.1:17482424-17482443 None:intergenic 55.0%
GCCTTCTAGTACTCCACACC+AGG + chr4.1:17480573-17480592 None:intergenic 55.0%
GGCAGGAATCGGAACTCTGT+TGG + chr4.1:17480266-17480285 None:intergenic 55.0%
TGTCTCAGGCCGTAGGAAGT+AGG + chr4.1:17482417-17482436 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 17480259 17483112 17480259 ID=MS.gene08393
chr4.1 mRNA 17480259 17483112 17480259 ID=MS.gene08393.t1;Parent=MS.gene08393
chr4.1 exon 17483050 17483112 17483050 ID=MS.gene08393.t1.exon1;Parent=MS.gene08393.t1
chr4.1 CDS 17483050 17483112 17483050 ID=cds.MS.gene08393.t1;Parent=MS.gene08393.t1
chr4.1 exon 17481743 17481824 17481743 ID=MS.gene08393.t1.exon2;Parent=MS.gene08393.t1
chr4.1 CDS 17481743 17481824 17481743 ID=cds.MS.gene08393.t1;Parent=MS.gene08393.t1
chr4.1 exon 17481049 17481131 17481049 ID=MS.gene08393.t1.exon3;Parent=MS.gene08393.t1
chr4.1 CDS 17481049 17481131 17481049 ID=cds.MS.gene08393.t1;Parent=MS.gene08393.t1
chr4.1 exon 17480259 17480423 17480259 ID=MS.gene08393.t1.exon4;Parent=MS.gene08393.t1
chr4.1 CDS 17480259 17480423 17480259 ID=cds.MS.gene08393.t1;Parent=MS.gene08393.t1
Gene Sequence

>MS.gene08393

ATGGCCAACAGAGTTCCGATTCCTGCCAACAATTCATCACTTATTGCCATGATTGCTGACGAGGTAAGCGCGTTCCCATGTTGTATATACTTTCTTATCAGTGATATGTTGTCTAAAAGAATGTCAAATCAGATGATATGATAACTAGGATGCATGATTGAATAGGGTTTAGCCGCAAAATCATCTTAGCTAATATAAGTTCGCTGCTAGAGAATTTTGTGATATTTTAAAGATCTATTTTGCTATTGGCGTACAACTCATTATGCACCACTGCACACTTCTTAAGAAAACAAACTATTATTGTACATTTTCCTGGTGTGGAGTACTAGAAGGCTTGGGGTTTAATAGGTTAGGTTTAGGTTCTCTTGAAAGGATCAAATTCAGGGTTTAGAAAATAAGTGGATGGATAGGTTCTCGAGGAAGGGACTTTCAGCCATAAGATGACTTAAGAAGTCAGATTGACGACATTTCAGATGCTGCCTAAAACCACTGATTTCCTTTTTTATTAATTGAGTTTTCCTTAAATAGATTCTAGCAAAAATTAACAGGATTTAAATGAAATAGGAAATTGTGAGGTCGTCCTCATCCAAAGAAACTAAATTTGTCTCTCTGATAAAATTGAAGAAAGGGATTTAGAGATCATAGGAAAAGAAGAAAATCATCATACATTGAGTTTGATTGCATCACACATAGTCAAGGAGGAGCAATATACTTCCATATTATATAAGCTGGTATTTTATTGATGTTTTTGATTGCCTTGTTAATGAAATAGGAGTATAAGTACCCTGTGATTTGTACCAAGCAGTCTGAAACTGCAGGTCCAATTACTCTAAGTCAAATCTAGCCAATTACTAGTATAAACGGAACCCTGAAATAAGGAAATAAAACACAAACTCCCCCAAAGCCAAAATCCATACAATGACAGTTGGAGTGTTGGACATGAGTATATGACTAGATACTTAACTATACAGTCCTAGCAGCACCGCACTAAAATATCCCTATGGCTGAAAACTGAAAGTATAATAATCTTTTGACATTATAAGTTTTCTAATCTTAAAATCAGATATAGTTCAGTCGGCAATTGAACATTACAACTGGCCCCTATTAATTTGAATAACTAGTGAGATATTATATTCTTTGAGCTTCTCTTTTTTATATTCTAATTTTGCTCATATCATTTCCCAATTCCATTTTAGTTTTTTTTTTTTTTTTTTTTGTAATTGGTATTCATTATGATTGAAATGATTAAAATGTTCTGAGATTTTGAATTCAATTTTATTTTCCACAGGATACCATAGTTGGGTTTCTTTTGGCTGGAGTGGGAAACGTTGACATACGTCGGAAGACAAATTACCTTATTGTGGATTCAAGTAAGTCACTAAACTATATTTACTTACTATTATTTATGAGGGCTAGTTTCGAATTCCCCCATACAATTTTTTCCTGTTAATCTGTGCATCATGTTCATGTGGAGACTCCCTGCCTTTTCACCTAATTTTCTGATTGTGGTAGATGTCCAATAAAAATAGTACATGTGTTCTTTTATGTTTTATAAAGACTTACTGTTGTATATCGTGGACCTCGTAAATAAGCATGGGGATATGTCTCTCCTATATAGGATGTGTAGAAATGTTGTGATTTCCACAAACCTTATCTTTAATACATTTCATAGCTTAGACACTTCCCAGAAAAACCGTAGAATACTTATTTTGGGCATGGGCTATTTTTTTATGCAAAGGTTATTAGTAGGTGCCCTGAAACTGAGGTTAATTGGTACTTGAACCTTGGGTAGTACTTGGATTACTCACTACAGATTGATAAGATAGCATCATTTTTTATTTTTATTTTAAATATAGCATCATTTTTTCTCTCTGAATAGCGTGTCAAGTTCTGAAAATATTCCTGGTAATTTCTTGCTCAGTTTCAAAACAAATTGATTTGACGGAAGTTTTTTGTTTTAACTCTTGATTTTGATCTTCAGAAACGACTGTCAAACAAATCGAGGATGCATTCAAAGAATTCTCAACACGGGAGGATATTGCAATTGTGCTGATTAGCCAATTTGTAAGTCATATTTCATGGCCATAAAAATGATTCAGGCTGTTTTGTTCTTGCTTCTTAGTTAAGTAAAACACCATACCTGCTACTGTTAGTTCCTACTTCCTACGGCCTGAGACATCAGCTTATTACGTCCAAAAGACTTTTTACTTATCCTTACCTATTATAGTTTAACACATGTTCTGTTTGGTAATCAACACTATTGTTAGGGAAAATCTGGAGGGAATATTTTAATTATCTACTGCATCAGGTTCCAAAACTTACATGAGGTTAATGAATAATGCTGGATAACTGGAGATTTTATTTGACAGACCAATGTGACAGTACCAGTGAGATTCAATCATAAAATTGGCACTTCTTTCGATAGCCATCCTTTGGGTTTAAATGTCGACAAGTTATATTATTGCAAAAAAATGTTGACTAGTTTATATGGTCATTGGTTTCTCACTGTTGGATCAGTTATTAGTTGGATATTTGGATCCGTCCTTTTAGCTCATTCAGCCATTGTGATATCCTAGCTGGGATTTAAAAACTATTTCATGCACCCGGTTCCTATTTAGAAAACCTTCTTGCTTATTTGGCCGTTACAATCTTGGGTTATTTATATCTTATAATTTCTTTGACTATGCAGGTTGCAAATATGATAAGGTTTTTGGTTGATAGTTATAACAAGCCAGTTCCTGCAATTCTTGAAATCCCTTCAAAAGACCACCCTTATGATCCTGCTCATGATTCAGTTCTTTCACGGGTGAAGTATCTCTTCAATGCTGAATCAGTGGCAGGTGATAGACGTTGA
Protein sequence

>MS.gene08393.t1

MANRVPIPANNSSLIAMIADEDTIVGFLLAGVGNVDIRRKTNYLIVDSKTTVKQIEDAFKEFSTREDIAIVLISQFVANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLFNAESVAGDRR