AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20715

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20715.t1 MTR_1g088775 98.867 353 4 0 1 353 1 353 0.0 730
MS.gene20715.t1 MTR_7g075090 92.351 353 27 0 1 353 1 353 0.0 694
MS.gene20715.t1 MTR_8g036040 64.306 353 118 1 1 353 1 345 2.46e-171 481
MS.gene20715.t1 MTR_8g036035 64.146 357 118 4 1 354 1 350 1.77e-161 456
MS.gene20715.t1 MTR_8g036020 69.000 300 92 1 55 354 18 316 5.36e-153 434
MS.gene20715.t1 MTR_8g036050 63.000 300 106 1 55 354 1 295 2.00e-140 401
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20715.t1 AT2G45440 75.706 354 85 1 1 354 1 353 0.0 561
MS.gene20715.t1 AT3G60880 74.788 353 88 1 1 353 1 352 0.0 548
MS.gene20715.t1 AT3G60880 74.504 353 88 1 1 353 1 351 0.0 546

Find 88 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAATAGAATTTGCCAATT+TGG 0.151527 1.2:+66471126 MS.gene20715:CDS
CCGCATTGGGAGGTGGAAAT+TGG 0.197311 1.2:-66469769 None:intergenic
ACCTATTTCCTTCACCAAAT+TGG 0.206485 1.2:-66471140 None:intergenic
GGTGGGAAAATCAAGGTTAT+TGG 0.223073 1.2:+66470530 MS.gene20715:CDS
GATTATTCAGGAACTCTTAC+TGG 0.297793 1.2:+66469721 MS.gene20715:intron
ATCAAGGTTATTGGAAATAC+TGG 0.303578 1.2:+66470539 MS.gene20715:CDS
GCCAATTTGGTGAAGGAAAT+AGG 0.310672 1.2:+66471139 MS.gene20715:CDS
GCGCACACAGTTAATTGTTT+TGG 0.323823 1.2:+66470509 MS.gene20715:CDS
GTTGCAAGCAATCTGATTCC+TGG 0.342044 1.2:+66470926 MS.gene20715:CDS
GTGTTCAAACCAATGGGATT+TGG 0.372649 1.2:-66471034 None:intergenic
TGTTGGAACTGAAGACGTTC+AGG 0.373512 1.2:+66471174 MS.gene20715:CDS
AGCTCTTGCCTTTGATTGAC+TGG 0.378010 1.2:+66470999 MS.gene20715:CDS
GCTACATTGAAGAGCTATAC+TGG 0.379000 1.2:+66469196 MS.gene20715:CDS
ATCAATGCCATGATGCTAGA+TGG 0.385109 1.2:+66470876 MS.gene20715:CDS
GGTGTCTGCTTCAGAGGATC+TGG 0.386418 1.2:+66469217 MS.gene20715:CDS
TTGCTCAACTTGGAGTTGTC+AGG 0.401383 1.2:+66471062 MS.gene20715:CDS
ACAAATGGCAACCTGAAAAC+TGG 0.401923 1.2:-66471085 None:intergenic
CTTTCAAAGTGTGCTTTCCA+TGG 0.406034 1.2:+66470682 MS.gene20715:CDS
AACACGGCTCTTGCTCAACT+TGG 0.407658 1.2:+66471052 MS.gene20715:CDS
TCATCTCAAAACTCCGCATT+GGG 0.420171 1.2:-66469782 None:intergenic
GACTTATTGTATCCTTTCTC+CGG 0.423830 1.2:+66471278 MS.gene20715:intron
CTTCATATAAACCCTTACTA+TGG 0.428403 1.2:+66470629 MS.gene20715:CDS
GGAGTTGAAGGTGTTATTGT+TGG 0.429517 1.2:+66470431 MS.gene20715:CDS
CATAGTAAGGGTTTATATGA+AGG 0.438848 1.2:-66470628 None:intergenic
TTACTATGGCAAAACCTCAT+TGG 0.439136 1.2:+66470643 MS.gene20715:CDS
TATAATGTGCCTTCACGAAC+TGG 0.441534 1.2:+66470719 MS.gene20715:CDS
GTTATTGTTGGTGGCACAAC+TGG 0.447799 1.2:+66470443 MS.gene20715:CDS
CTTAGCTCAAAATACTTGCT+TGG 0.461095 1.2:+66470772 MS.gene20715:CDS
GCTCAAAATACTTGCTTGGC+TGG 0.463469 1.2:+66470776 MS.gene20715:CDS
TTCATCTCAAAACTCCGCAT+TGG 0.466411 1.2:-66469783 None:intergenic
GTGAAGGTCAATTAATGAGC+TGG 0.470889 1.2:+66470465 MS.gene20715:CDS
ATGCGAGAACTCATGTTTGA+GGG 0.476788 1.2:+66470953 MS.gene20715:CDS
AGATGGGGTTATGGAGCTAC+TGG 0.478069 1.2:+66470893 MS.gene20715:CDS
TCAAGATCAAATCGACCATC+AGG 0.480309 1.2:-66470371 None:intergenic
GTTGAAGGTGTTATTGTTGG+TGG 0.483414 1.2:+66470434 MS.gene20715:CDS
CAAAACTCCGCATTGGGAGG+TGG 0.487292 1.2:-66469776 None:intergenic
CATGATGCTAGATGGGGTTA+TGG 0.493610 1.2:+66470884 MS.gene20715:CDS
TACTTGCTTGGCTGGTGTCA+AGG 0.500800 1.2:+66470784 MS.gene20715:CDS
CAGATCGAGAGTGGAGTTGA+AGG 0.501168 1.2:+66470419 MS.gene20715:CDS
ATAGAATCGTTGTGTGGAGC+GGG 0.501345 1.2:+66470846 MS.gene20715:CDS
AATGCGAGAACTCATGTTTG+AGG 0.508177 1.2:+66470952 MS.gene20715:CDS
GTAAGATCGGAAGGATGAAC+CGG 0.510311 1.2:-66469244 None:intergenic
AACCAATGGGATTTGGCATG+TGG 0.510972 1.2:-66471027 None:intergenic
TATACTGGTGTCTGCTTCAG+AGG 0.514396 1.2:+66469211 MS.gene20715:CDS
GCAATTCATGCCACTGAGCA+AGG 0.519046 1.2:+66470581 MS.gene20715:CDS
TTTCAAAGTGTGCTTTCCAT+GGG 0.520623 1.2:+66470683 MS.gene20715:CDS
GATTTCACAGCTGCTTGTGT+AGG 0.522599 1.2:-66469747 None:intergenic
AAGAGCTTAGAATTCAATGT+AGG 0.522936 1.2:-66470983 None:intergenic
AAGAGCCGTGTTCAAACCAA+TGG 0.526094 1.2:-66471041 None:intergenic
TCTAATGGGAGAGGTACAAA+TGG 0.526400 1.2:-66471100 None:intergenic
AATCGACCATCAGGTAGGTA+TGG 0.528759 1.2:-66470362 None:intergenic
AGAATTTGCCAATTTGGTGA+AGG 0.533742 1.2:+66471132 MS.gene20715:CDS
CAAAACCTCATTGGATGGTA+TGG 0.535451 1.2:+66470652 MS.gene20715:CDS
AGGAATGTGTGGGAAATGAC+AGG 0.543586 1.2:+66470804 MS.gene20715:CDS
TCTTGAAGCATACGATGCCT+TGG 0.545550 1.2:+66470388 MS.gene20715:CDS
TTCCACATGCCAAATCCCAT+TGG 0.545798 1.2:+66471025 MS.gene20715:CDS
GGCTGGTGTCAAGGAATGTG+TGG 0.556357 1.2:+66470793 MS.gene20715:CDS
CATAACCCCATCTAGCATCA+TGG 0.567567 1.2:-66470883 None:intergenic
TTGTTAGTAGTAAGATCGGA+AGG 0.567613 1.2:-66469253 None:intergenic
TCAATGCCATGATGCTAGAT+GGG 0.567969 1.2:+66470877 MS.gene20715:CDS
TGGAAAAGCCAGTCAATCAA+AGG 0.572095 1.2:-66471007 None:intergenic
TATGGCAAAACCTCATTGGA+TGG 0.575275 1.2:+66470647 MS.gene20715:CDS
ATATATGATCGTGGGTCCCA+TGG 0.576230 1.2:-66470699 None:intergenic
ATAGTAAGGGTTTATATGAA+GGG 0.577466 1.2:-66470627 None:intergenic
AGAGCCGTGTTCAAACCAAT+GGG 0.577523 1.2:-66471040 None:intergenic
GTAAATATGCAGATCGAGAG+TGG 0.578320 1.2:+66470410 MS.gene20715:CDS
TGAAGGTCAATTAATGAGCT+GGG 0.584644 1.2:+66470466 MS.gene20715:CDS
TATTTACAGGACTTGTACAG+AGG 0.599967 1.2:+66470301 MS.gene20715:intron
ATGAGTTCTCGCATTAAACC+AGG 0.602598 1.2:-66470944 None:intergenic
AATAGAATCGTTGTGTGGAG+CGG 0.613867 1.2:+66470845 MS.gene20715:CDS
AAATCCCATTGGTTTGAACA+CGG 0.620889 1.2:+66471036 MS.gene20715:CDS
TCTCAAAACTCCGCATTGGG+AGG 0.623035 1.2:-66469779 None:intergenic
GGCACATTATATATGATCGT+GGG 0.623516 1.2:-66470707 None:intergenic
AGGCACATTATATATGATCG+TGG 0.625176 1.2:-66470708 None:intergenic
AAAACTCCATACCTACCTGA+TGG 0.627623 1.2:+66470356 MS.gene20715:CDS
ATACTGGAAGTAACTCGACA+AGG 0.635535 1.2:+66470555 MS.gene20715:CDS
TACTGGAAGTAACTCGACAA+GGG 0.637824 1.2:+66470556 MS.gene20715:CDS
CTCGATCTGCATATTTACCA+AGG 0.639513 1.2:-66470405 None:intergenic
ATGTCTTGACCAGTTCGTGA+AGG 0.650374 1.2:-66470728 None:intergenic
TATTCAGGAACTCTTACTGG+AGG 0.652852 1.2:+66469724 MS.gene20715:intron
GTTGGTGGCACAACTGGTGA+AGG 0.663404 1.2:+66470449 MS.gene20715:CDS
GCTGGTGTCAAGGAATGTGT+GGG 0.663954 1.2:+66470794 MS.gene20715:CDS
CTGATAATAGAATCGTTGTG+TGG 0.669847 1.2:+66470840 MS.gene20715:CDS
AACCGCAAAACCTTGCTCAG+TGG 0.673625 1.2:-66470591 None:intergenic
GATCAAATCGACCATCAGGT+AGG 0.678861 1.2:-66470367 None:intergenic
AGCAACCATACCATCCAATG+AGG 0.685283 1.2:-66470657 None:intergenic
CCAATTTCCACCTCCCAATG+CGG 0.691962 1.2:+66469769 MS.gene20715:CDS
CAATGCCATGATGCTAGATG+GGG 0.711874 1.2:+66470878 MS.gene20715:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATTTCAAAATAAAGAAAA+CGG - chr1.2:66469299-66469318 None:intergenic 15.0%
!! AAGCTATATATTGTATTTAC+AGG + chr1.2:66470288-66470307 MS.gene20715:intron 20.0%
!! AATGAATTCGATGAAATAAA+CGG + chr1.2:66469462-66469481 MS.gene20715:intron 20.0%
!! TGTTAGTTATTTATTGAGTT+AGG + chr1.2:66471245-66471264 MS.gene20715:intron 20.0%
!!! AATTCTATTCTTTTCTCTAA+TGG - chr1.2:66471118-66471137 None:intergenic 20.0%
!!! ATGATTTTAATGATGATGAA+GGG + chr1.2:66469510-66469529 MS.gene20715:intron 20.0%
!!! ATTCTATTCTTTTCTCTAAT+GGG - chr1.2:66471117-66471136 None:intergenic 20.0%
!!! GTTTTCATCTTTTTATTTTC+TGG + chr1.2:66469939-66469958 MS.gene20715:intron 20.0%
!!! TATGATTTTAATGATGATGA+AGG + chr1.2:66469509-66469528 MS.gene20715:intron 20.0%
!!! TTCTAAGGAGTATATTTAAA+AGG + chr1.2:66470187-66470206 MS.gene20715:intron 20.0%
! AAGAATAGAATTTGCCAATT+TGG + chr1.2:66471126-66471145 MS.gene20715:CDS 25.0%
! AATCTCAAATTGAATGATAG+AGG - chr1.2:66470070-66470089 None:intergenic 25.0%
! AGTATCAAAACTAATGAATG+TGG - chr1.2:66469841-66469860 None:intergenic 25.0%
! ATAGTAAGGGTTTATATGAA+GGG - chr1.2:66470630-66470649 None:intergenic 25.0%
! GAAAAACAAATCCAAATTAG+TGG - chr1.2:66470048-66470067 None:intergenic 25.0%
!! AATCATGGAATCACAAATTT+TGG + chr1.2:66470258-66470277 MS.gene20715:intron 25.0%
!! GTTTTTGTTAGTAGTAAGAT+CGG - chr1.2:66469260-66469279 None:intergenic 25.0%
!! TAAGGAGTATATTTAAAAGG+TGG + chr1.2:66470190-66470209 MS.gene20715:intron 25.0%
!! TATTGTGATTTGTGATGAAA+CGG + chr1.2:66469992-66470011 MS.gene20715:intron 25.0%
!! TGGTTAGTCGATATTAATTT+AGG + chr1.2:66471221-66471240 MS.gene20715:intron 25.0%
!! TTGAGAGTATAATTTAGCTA+AGG + chr1.2:66469561-66469580 MS.gene20715:intron 25.0%
!!! AGATTTTGATTTGACATACA+AGG + chr1.2:66470085-66470104 MS.gene20715:intron 25.0%
!!! TGTTGCTTTTGTTTTAATCA+TGG + chr1.2:66470243-66470262 MS.gene20715:intron 25.0%
!!! TTTTGAGATGAAAAATAGGT+TGG + chr1.2:66469794-66469813 MS.gene20715:intron 25.0%
AAGAGCTTAGAATTCAATGT+AGG - chr1.2:66470986-66471005 None:intergenic 30.0%
AAGGATACAATAAGTCTACA+AGG - chr1.2:66471274-66471293 None:intergenic 30.0%
ACAAAAGCAACAACACAAAT+AGG - chr1.2:66470235-66470254 None:intergenic 30.0%
AGCACAATCAATATCAATCA+CGG - chr1.2:66469639-66469658 None:intergenic 30.0%
ATCAAGGTTATTGGAAATAC+TGG + chr1.2:66470539-66470558 MS.gene20715:CDS 30.0%
CATAGTAAGGGTTTATATGA+AGG - chr1.2:66470631-66470650 None:intergenic 30.0%
CTTCATATAAACCCTTACTA+TGG + chr1.2:66470629-66470648 MS.gene20715:CDS 30.0%
CTTGCTTATTTCCACTAATT+TGG + chr1.2:66470034-66470053 MS.gene20715:intron 30.0%
GTTTGTCTGTATGATTATTC+AGG + chr1.2:66469709-66469728 MS.gene20715:intron 30.0%
TTACTACTAACAAAAACACC+AGG + chr1.2:66469263-66469282 MS.gene20715:CDS 30.0%
! AGATTTTGTTAGCATGTATG+AGG + chr1.2:66469612-66469631 MS.gene20715:intron 30.0%
! ATTGTGATTTGTGATGAAAC+GGG + chr1.2:66469993-66470012 MS.gene20715:intron 30.0%
! CAAAATCTAAGATACAGGAA+TGG - chr1.2:66469600-66469619 None:intergenic 30.0%
! GCTAACAAAATCTAAGATAC+AGG - chr1.2:66469605-66469624 None:intergenic 30.0%
!! ATTGCAGCTGAATTTGATTT+TGG + chr1.2:66469406-66469425 MS.gene20715:intron 30.0%
!! TTGATTTGTCTATATGCATG+TGG + chr1.2:66469383-66469402 MS.gene20715:intron 30.0%
!!! ACACAGTTAATTGTTTTGGT+GGG + chr1.2:66470513-66470532 MS.gene20715:CDS 30.0%
!!! GCTAAGTTTTGAATTACATG+CGG - chr1.2:66470758-66470777 None:intergenic 30.0%
!!! GGAGTTTTGAGATGAAAAAT+AGG + chr1.2:66469790-66469809 MS.gene20715:CDS 30.0%
AAGCATTATTCCACTAAGTG+GGG + chr1.2:66469876-66469895 MS.gene20715:intron 35.0%
ACCTATTTCCTTCACCAAAT+TGG - chr1.2:66471143-66471162 None:intergenic 35.0%
AGGCACATTATATATGATCG+TGG - chr1.2:66470711-66470730 None:intergenic 35.0%
CAAGCATTATTCCACTAAGT+GGG + chr1.2:66469875-66469894 MS.gene20715:intron 35.0%
CTGATAATAGAATCGTTGTG+TGG + chr1.2:66470840-66470859 MS.gene20715:CDS 35.0%
CTTAGCTCAAAATACTTGCT+TGG + chr1.2:66470772-66470791 MS.gene20715:CDS 35.0%
GAAAACGGAAAATTTGAACC+TGG - chr1.2:66469284-66469303 None:intergenic 35.0%
GACTTATTGTATCCTTTCTC+CGG + chr1.2:66471278-66471297 MS.gene20715:intron 35.0%
GATTATTCAGGAACTCTTAC+TGG + chr1.2:66469721-66469740 MS.gene20715:intron 35.0%
GCACAATCAATATCAATCAC+GGG - chr1.2:66469638-66469657 None:intergenic 35.0%
GGCACATTATATATGATCGT+GGG - chr1.2:66470710-66470729 None:intergenic 35.0%
TATTTACAGGACTTGTACAG+AGG + chr1.2:66470301-66470320 MS.gene20715:intron 35.0%
TGAAGGTCAATTAATGAGCT+GGG + chr1.2:66470466-66470485 MS.gene20715:CDS 35.0%
TTACTATGGCAAAACCTCAT+TGG + chr1.2:66470643-66470662 MS.gene20715:CDS 35.0%
TTGTTAGTAGTAAGATCGGA+AGG - chr1.2:66469256-66469275 None:intergenic 35.0%
TTTCAAAGTGTGCTTTCCAT+GGG + chr1.2:66470683-66470702 MS.gene20715:CDS 35.0%
! AATGAGGTTTTGCCATAGTA+AGG - chr1.2:66470644-66470663 None:intergenic 35.0%
! AGAATTTGCCAATTTGGTGA+AGG + chr1.2:66471132-66471151 MS.gene20715:CDS 35.0%
! ATAGGTCGTCAACATTTTGT+TGG + chr1.2:66471157-66471176 MS.gene20715:CDS 35.0%
! ATGAGGTTTTGCCATAGTAA+GGG - chr1.2:66470643-66470662 None:intergenic 35.0%
! ATTCTTTTCTCTAATGGGAG+AGG - chr1.2:66471112-66471131 None:intergenic 35.0%
! TTCTGGAAGAGACACAAATA+TGG + chr1.2:66469956-66469975 MS.gene20715:intron 35.0%
!! AAATCCCATTGGTTTGAACA+CGG + chr1.2:66471036-66471055 MS.gene20715:CDS 35.0%
!! AGGTAGGTATGGAGTTTTAA+TGG - chr1.2:66470354-66470373 None:intergenic 35.0%
!! TGATGACAACGACTTTTTCT+TGG + chr1.2:66471201-66471220 MS.gene20715:intron 35.0%
!!! AAGTTTTGAATTACATGCGG+TGG - chr1.2:66470755-66470774 None:intergenic 35.0%
!!! CACACAGTTAATTGTTTTGG+TGG + chr1.2:66470512-66470531 MS.gene20715:CDS 35.0%
!!! TTGTTTTGGTGGGAAAATCA+AGG + chr1.2:66470523-66470542 MS.gene20715:CDS 35.0%
AAAACTCCATACCTACCTGA+TGG + chr1.2:66470356-66470375 MS.gene20715:CDS 40.0%
AATAGAATCGTTGTGTGGAG+CGG + chr1.2:66470845-66470864 MS.gene20715:CDS 40.0%
AATGCGAGAACTCATGTTTG+AGG + chr1.2:66470952-66470971 MS.gene20715:CDS 40.0%
ACAAATGGCAACCTGAAAAC+TGG - chr1.2:66471088-66471107 None:intergenic 40.0%
ATACTCCTTAGAACATGCAG+CGG - chr1.2:66470180-66470199 None:intergenic 40.0%
ATACTGGAAGTAACTCGACA+AGG + chr1.2:66470555-66470574 MS.gene20715:CDS 40.0%
ATCAATGCCATGATGCTAGA+TGG + chr1.2:66470876-66470895 MS.gene20715:CDS 40.0%
ATGAGTTCTCGCATTAAACC+AGG - chr1.2:66470947-66470966 None:intergenic 40.0%
ATGCGAGAACTCATGTTTGA+GGG + chr1.2:66470953-66470972 MS.gene20715:CDS 40.0%
ATTATTCCACTAAGTGGGGT+TGG + chr1.2:66469880-66469899 MS.gene20715:intron 40.0%
CAAAACCTCATTGGATGGTA+TGG + chr1.2:66470652-66470671 MS.gene20715:CDS 40.0%
CCAAGCATTATTCCACTAAG+TGG + chr1.2:66469874-66469893 MS.gene20715:intron 40.0%
CCACTTAGTGGAATAATGCT+TGG - chr1.2:66469877-66469896 None:intergenic 40.0%
CTCGATCTGCATATTTACCA+AGG - chr1.2:66470408-66470427 None:intergenic 40.0%
CTTTCAAAGTGTGCTTTCCA+TGG + chr1.2:66470682-66470701 MS.gene20715:CDS 40.0%
GCTACATTGAAGAGCTATAC+TGG + chr1.2:66469196-66469215 MS.gene20715:CDS 40.0%
GGTGGGAAAATCAAGGTTAT+TGG + chr1.2:66470530-66470549 MS.gene20715:CDS 40.0%
GTAAATATGCAGATCGAGAG+TGG + chr1.2:66470410-66470429 MS.gene20715:CDS 40.0%
GTGAAGGTCAATTAATGAGC+TGG + chr1.2:66470465-66470484 MS.gene20715:CDS 40.0%
GTGTTCAAACCAATGGGATT+TGG - chr1.2:66471037-66471056 None:intergenic 40.0%
TACTGGAAGTAACTCGACAA+GGG + chr1.2:66470556-66470575 MS.gene20715:CDS 40.0%
TATAATGTGCCTTCACGAAC+TGG + chr1.2:66470719-66470738 MS.gene20715:CDS 40.0%
TATGGCAAAACCTCATTGGA+TGG + chr1.2:66470647-66470666 MS.gene20715:CDS 40.0%
TATTCAGGAACTCTTACTGG+AGG + chr1.2:66469724-66469743 MS.gene20715:intron 40.0%
TCAAGATCAAATCGACCATC+AGG - chr1.2:66470374-66470393 None:intergenic 40.0%
TCAATGCCATGATGCTAGAT+GGG + chr1.2:66470877-66470896 MS.gene20715:CDS 40.0%
TCATCTCAAAACTCCGCATT+GGG - chr1.2:66469785-66469804 None:intergenic 40.0%
TGGAAAAGCCAGTCAATCAA+AGG - chr1.2:66471010-66471029 None:intergenic 40.0%
TTCATCTCAAAACTCCGCAT+TGG - chr1.2:66469786-66469805 None:intergenic 40.0%
! GCCAATTTGGTGAAGGAAAT+AGG + chr1.2:66471139-66471158 MS.gene20715:CDS 40.0%
! GCGCACACAGTTAATTGTTT+TGG + chr1.2:66470509-66470528 MS.gene20715:CDS 40.0%
! TCTAATGGGAGAGGTACAAA+TGG - chr1.2:66471103-66471122 None:intergenic 40.0%
!! GGAGTTGAAGGTGTTATTGT+TGG + chr1.2:66470431-66470450 MS.gene20715:CDS 40.0%
!! GTTGAAGGTGTTATTGTTGG+TGG + chr1.2:66470434-66470453 MS.gene20715:CDS 40.0%
AACCAATGGGATTTGGCATG+TGG - chr1.2:66471030-66471049 None:intergenic 45.0%
AAGAGCCGTGTTCAAACCAA+TGG - chr1.2:66471044-66471063 None:intergenic 45.0%
AATCGACCATCAGGTAGGTA+TGG - chr1.2:66470365-66470384 None:intergenic 45.0%
AGAGCCGTGTTCAAACCAAT+GGG - chr1.2:66471043-66471062 None:intergenic 45.0%
AGCAACCATACCATCCAATG+AGG - chr1.2:66470660-66470679 None:intergenic 45.0%
AGCTCTTGCCTTTGATTGAC+TGG + chr1.2:66470999-66471018 MS.gene20715:CDS 45.0%
AGGAATGTGTGGGAAATGAC+AGG + chr1.2:66470804-66470823 MS.gene20715:CDS 45.0%
ATAGAATCGTTGTGTGGAGC+GGG + chr1.2:66470846-66470865 MS.gene20715:CDS 45.0%
ATATATGATCGTGGGTCCCA+TGG - chr1.2:66470702-66470721 None:intergenic 45.0%
ATGTCTTGACCAGTTCGTGA+AGG - chr1.2:66470731-66470750 None:intergenic 45.0%
CAATGCCATGATGCTAGATG+GGG + chr1.2:66470878-66470897 MS.gene20715:CDS 45.0%
CATAACCCCATCTAGCATCA+TGG - chr1.2:66470886-66470905 None:intergenic 45.0%
CATGATGCTAGATGGGGTTA+TGG + chr1.2:66470884-66470903 MS.gene20715:CDS 45.0%
GATCAAATCGACCATCAGGT+AGG - chr1.2:66470370-66470389 None:intergenic 45.0%
GATTTCACAGCTGCTTGTGT+AGG - chr1.2:66469750-66469769 None:intergenic 45.0%
GCTCAAAATACTTGCTTGGC+TGG + chr1.2:66470776-66470795 MS.gene20715:CDS 45.0%
GTAAGATCGGAAGGATGAAC+CGG - chr1.2:66469247-66469266 None:intergenic 45.0%
GTTGCAAGCAATCTGATTCC+TGG + chr1.2:66470926-66470945 MS.gene20715:CDS 45.0%
TATACTGGTGTCTGCTTCAG+AGG + chr1.2:66469211-66469230 MS.gene20715:CDS 45.0%
TCTTGAAGCATACGATGCCT+TGG + chr1.2:66470388-66470407 MS.gene20715:CDS 45.0%
TGTTGGAACTGAAGACGTTC+AGG + chr1.2:66471174-66471193 MS.gene20715:CDS 45.0%
TTCCACATGCCAAATCCCAT+TGG + chr1.2:66471025-66471044 MS.gene20715:CDS 45.0%
TTGCTCAACTTGGAGTTGTC+AGG + chr1.2:66471062-66471081 MS.gene20715:CDS 45.0%
!! GTTATTGTTGGTGGCACAAC+TGG + chr1.2:66470443-66470462 MS.gene20715:CDS 45.0%
!!! CTTCAGAGGATCTGGTTTTC+CGG + chr1.2:66469225-66469244 MS.gene20715:CDS 45.0%
!! AAACATTAAATAAAAAAAAT+TGG - chr1.2:66469693-66469712 None:intergenic 5.0%
AACACGGCTCTTGCTCAACT+TGG + chr1.2:66471052-66471071 MS.gene20715:CDS 50.0%
AACCGCAAAACCTTGCTCAG+TGG - chr1.2:66470594-66470613 None:intergenic 50.0%
AGATGGGGTTATGGAGCTAC+TGG + chr1.2:66470893-66470912 MS.gene20715:CDS 50.0%
CAGATCGAGAGTGGAGTTGA+AGG + chr1.2:66470419-66470438 MS.gene20715:CDS 50.0%
CCAATTTCCACCTCCCAATG+CGG + chr1.2:66469769-66469788 MS.gene20715:CDS 50.0%
CTAATGCCAACCCCACTTAG+TGG - chr1.2:66469889-66469908 None:intergenic 50.0%
CTGTACCGCTGCATGTTCTA+AGG + chr1.2:66470172-66470191 MS.gene20715:intron 50.0%
GCAATTCATGCCACTGAGCA+AGG + chr1.2:66470581-66470600 MS.gene20715:CDS 50.0%
TCTCAAAACTCCGCATTGGG+AGG - chr1.2:66469782-66469801 None:intergenic 50.0%
! ACTGAGCAAGGTTTTGCGGT+TGG + chr1.2:66470593-66470612 MS.gene20715:CDS 50.0%
! GAGTTGTCAGGCCAGTTTTC+AGG + chr1.2:66471074-66471093 MS.gene20715:CDS 50.0%
! GCTGGTGTCAAGGAATGTGT+GGG + chr1.2:66470794-66470813 MS.gene20715:CDS 50.0%
! GGTTTTGCGGTTGGAATGCA+TGG + chr1.2:66470602-66470621 MS.gene20715:CDS 50.0%
! TACTTGCTTGGCTGGTGTCA+AGG + chr1.2:66470784-66470803 MS.gene20715:CDS 50.0%
! TGCCACTGAGCAAGGTTTTG+CGG + chr1.2:66470589-66470608 MS.gene20715:CDS 50.0%
AAGGTGGTCTCGCTTGCTTC+TGG + chr1.2:66470206-66470225 MS.gene20715:intron 55.0%
CAAAACTCCGCATTGGGAGG+TGG - chr1.2:66469779-66469798 None:intergenic 55.0%
CCGCATTGGGAGGTGGAAAT+TGG - chr1.2:66469772-66469791 None:intergenic 55.0%
GGTGTCTGCTTCAGAGGATC+TGG + chr1.2:66469217-66469236 MS.gene20715:CDS 55.0%
! GGCTGGTGTCAAGGAATGTG+TGG + chr1.2:66470793-66470812 MS.gene20715:CDS 55.0%
!! GTTGGTGGCACAACTGGTGA+AGG + chr1.2:66470449-66470468 MS.gene20715:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 66469193 66471311 66469193 ID=MS.gene20715
chr1.2 mRNA 66469193 66471311 66469193 ID=MS.gene20715.t1;Parent=MS.gene20715
chr1.2 exon 66469193 66469284 66469193 ID=MS.gene20715.t1.exon1;Parent=MS.gene20715.t1
chr1.2 CDS 66469193 66469284 66469193 ID=cds.MS.gene20715.t1;Parent=MS.gene20715.t1
chr1.2 exon 66469731 66469811 66469731 ID=MS.gene20715.t1.exon2;Parent=MS.gene20715.t1
chr1.2 CDS 66469731 66469811 66469731 ID=cds.MS.gene20715.t1;Parent=MS.gene20715.t1
chr1.2 exon 66470310 66471195 66470310 ID=MS.gene20715.t1.exon3;Parent=MS.gene20715.t1
chr1.2 CDS 66470310 66471195 66470310 ID=cds.MS.gene20715.t1;Parent=MS.gene20715.t1
chr1.2 exon 66471279 66471311 66471279 ID=MS.gene20715.t1.exon4;Parent=MS.gene20715.t1
chr1.2 CDS 66471279 66471311 66471279 ID=cds.MS.gene20715.t1;Parent=MS.gene20715.t1
Gene Sequence

>MS.gene20715

ATGGCTACATTGAAGAGCTATACTGGTGTCTGCTTCAGAGGATCTGGTTTTCCGGTTCATCCTTCCGATCTTACTACTAACAAAAACACCAGGTTCAAATTTTCCGTTTTCTTTATTTTGAAATCTGAATGACTGAAATGTATTTATGCATGTTTATTGAATTTATTTTTGAACATTTTTTTTGTTCTGTTTGATTTGTCTATATGCATGTGGATTGCAGCTGAATTTGATTTTGGAATGAATGAAAAAAATATGTCATTTGTGAAAATAATGAATTCGATGAAATAAACGGAGTTTCAAATGCATATTTGATATGTATGATTTTAATGATGATGAAGGGTATGCTTTGAGTCTACTATGTCTTTGCATTGAGAGTATAATTTAGCTAAGGTTGATACATATGACCATTCCTGTATCTTAGATTTTGTTAGCATGTATGAGGCCCGTGATTGATATTGATTGTGCTGTTGATAACATTTCAATCTTGTATTATCAATCCAATTTTTTTTATTTAATGTTTGTCTGTATGATTATTCAGGAACTCTTACTGGAGGCCTACACAAGCAGCTGTGAAATCCAATTTCCACCTCCCAATGCGGAGTTTTGAGATGAAAAATAGGTTGGTTGAATAATGAATTTACTGTGCCACATTCATTAGTTTTGATACTACAGCATAAAAAACCAAGCATTATTCCACTAAGTGGGGTTGGCATTAGTTTTCATACTAAATTATATATTTTTCATTTGTTTTCATCTTTTTATTTTCTGGAAGAGACACAAATATGGTTTTACAATATGATATTGTGATTTGTGATGAAACGGGAGATTTAAACAATAGCTTCTTGCTTATTTCCACTAATTTGGATTTGTTTTTCCTCTATCATTCAATTTGAGATTTTGATTTGACATACAAGGTTGACTAGTTCTTTGATCTGCAAAGTTATTTTACATTGATTTATATTTTTTTTATAAACTGCAACTGTACCGCTGCATGTTCTAAGGAGTATATTTAAAAGGTGGTCTCGCTTGCTTCTGGCTGCCTATTTGTGTTGTTGCTTTTGTTTTAATCATGGAATCACAAATTTTGGACTAATGAAGCTATATATTGTATTTACAGGACTTGTACAGAGGACATTAAGTGTCTGAGATTGATAACTGCCATTAAAACTCCATACCTACCTGATGGTCGATTTGATCTTGAAGCATACGATGCCTTGGTAAATATGCAGATCGAGAGTGGAGTTGAAGGTGTTATTGTTGGTGGCACAACTGGTGAAGGTCAATTAATGAGCTGGGAAGAGCACATAATGCTTATTGCGCACACAGTTAATTGTTTTGGTGGGAAAATCAAGGTTATTGGAAATACTGGAAGTAACTCGACAAGGGAAGCAATTCATGCCACTGAGCAAGGTTTTGCGGTTGGAATGCATGGTGCCCTTCATATAAACCCTTACTATGGCAAAACCTCATTGGATGGTATGGTTGCTCACTTTCAAAGTGTGCTTTCCATGGGACCCACGATCATATATAATGTGCCTTCACGAACTGGTCAAGACATTCCACCGCATGTAATTCAAAACTTAGCTCAAAATACTTGCTTGGCTGGTGTCAAGGAATGTGTGGGAAATGACAGGATCAAAGAGTATACTGATAATAGAATCGTTGTGTGGAGCGGGAACGATGATCAATGCCATGATGCTAGATGGGGTTATGGAGCTACTGGAGTGATATCTGTTGCAAGCAATCTGATTCCTGGTTTAATGCGAGAACTCATGTTTGAGGGCAAAAATCCTACATTGAATTCTAAGCTCTTGCCTTTGATTGACTGGCTTTTCCACATGCCAAATCCCATTGGTTTGAACACGGCTCTTGCTCAACTTGGAGTTGTCAGGCCAGTTTTCAGGTTGCCATTTGTACCTCTCCCATTAGAGAAAAGAATAGAATTTGCCAATTTGGTGAAGGAAATAGGTCGTCAACATTTTGTTGGAACTGAAGACGTTCAGGTTCTTGATGACAACGACTTTTTCTTGGTTAGTCGATATTAATTTAGGTTGTTAGTTATTTATTGAGTTAGGTCACCTTGTAGACTTATTGTATCCTTTCTCCGGTTTTTTTGTGA
Protein sequence

>MS.gene20715.t1

MATLKSYTGVCFRGSGFPVHPSDLTTNKNTRNSYWRPTQAAVKSNFHLPMRSFEMKNRTCTEDIKCLRLITAIKTPYLPDGRFDLEAYDALVNMQIESGVEGVIVGGTTGEGQLMSWEEHIMLIAHTVNCFGGKIKVIGNTGSNSTREAIHATEQGFAVGMHGALHINPYYGKTSLDGMVAHFQSVLSMGPTIIYNVPSRTGQDIPPHVIQNLAQNTCLAGVKECVGNDRIKEYTDNRIVVWSGNDDQCHDARWGYGATGVISVASNLIPGLMRELMFEGKNPTLNSKLLPLIDWLFHMPNPIGLNTALAQLGVVRPVFRLPFVPLPLEKRIEFANLVKEIGRQHFVGTEDVQTYCILSPVFL