AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20731

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20731.t1 MTR_1g088640 96.471 170 3 1 1 170 1 167 2.96e-117 328
MS.gene20731.t1 MTR_1g088640 94.706 170 3 2 1 170 1 164 6.83e-113 318
MS.gene20731.t1 MTR_7g108830 67.683 164 48 3 8 170 3 162 1.89e-67 202
MS.gene20731.t1 MTR_8g058620 67.683 164 48 3 8 170 3 162 1.89e-67 202
MS.gene20731.t1 MTR_1g088620 100.000 69 0 0 102 170 218 286 5.66e-42 142
MS.gene20731.t1 MTR_1g087200 40.252 159 90 3 11 166 2 158 1.07e-33 117
MS.gene20731.t1 MTR_1g054765 40.462 173 79 5 18 170 6 174 1.60e-32 114
MS.gene20731.t1 MTR_1g083950 39.474 152 89 2 17 168 11 159 1.10e-31 112
MS.gene20731.t1 MTR_1g054750 39.394 165 86 4 18 170 25 187 4.12e-30 108
MS.gene20731.t1 MTR_5g065980 32.716 162 96 3 16 164 9 170 8.36e-22 87.0
MS.gene20731.t1 MTR_5g065980 32.298 161 96 3 16 164 9 168 2.35e-21 85.9
MS.gene20731.t1 MTR_2g089350 29.545 176 106 5 7 167 12 184 1.59e-16 74.3
MS.gene20731.t1 MTR_4g064933 32.258 155 92 4 19 165 9 158 2.18e-15 70.1
MS.gene20731.t1 MTR_6g006190 24.051 158 109 3 17 165 8 163 1.32e-12 62.8
MS.gene20731.t1 MTR_5g077130 29.032 155 95 5 19 164 9 157 3.58e-12 61.6
MS.gene20731.t1 MTR_5g077130 28.846 156 95 5 19 164 9 158 4.97e-12 61.2
MS.gene20731.t1 MTR_2g034200 28.931 159 102 5 19 170 9 163 7.47e-12 60.5
MS.gene20731.t1 MTR_7g065770 29.299 157 92 5 19 164 9 157 1.58e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20731.t1 AT2G47710 63.522 159 53 2 13 169 6 161 2.27e-69 207
MS.gene20731.t1 AT3G11930 41.566 166 77 6 17 167 35 195 2.36e-33 117
MS.gene20731.t1 AT3G11930 41.317 167 77 6 17 167 35 196 3.04e-33 117
MS.gene20731.t1 AT3G11930 41.071 168 77 6 17 167 35 197 4.86e-33 116
MS.gene20731.t1 AT1G09740 38.365 159 88 5 17 167 10 166 5.89e-28 102
MS.gene20731.t1 AT1G09740 38.365 159 88 5 17 167 10 166 5.89e-28 102
MS.gene20731.t1 AT3G11930 35.751 193 77 7 17 167 35 222 4.45e-27 102
MS.gene20731.t1 AT5G49050 43.590 117 61 2 13 127 37 150 8.75e-26 96.7
MS.gene20731.t1 AT3G58450 37.349 166 83 5 17 167 32 191 1.45e-25 97.4
MS.gene20731.t1 AT3G62550 36.970 165 86 7 10 165 2 157 1.64e-25 96.3
MS.gene20731.t1 AT3G58450 36.145 166 78 6 17 167 32 184 1.01e-24 95.1
MS.gene20731.t1 AT1G11360 32.386 176 95 6 9 169 32 198 2.72e-19 82.0
MS.gene20731.t1 AT1G11360 32.386 176 95 6 9 169 32 198 2.72e-19 82.0
MS.gene20731.t1 AT1G11360 32.386 176 95 6 9 169 32 198 2.72e-19 82.0
MS.gene20731.t1 AT1G11360 32.386 176 95 6 9 169 32 198 2.72e-19 82.0
MS.gene20731.t1 AT1G68300 32.075 159 101 4 10 164 2 157 2.41e-18 77.8
MS.gene20731.t1 AT5G14680 27.647 170 99 5 12 165 2 163 4.29e-16 72.0
MS.gene20731.t1 AT3G01520 28.676 136 95 2 30 165 30 163 6.46e-15 68.9
MS.gene20731.t1 AT3G53990 25.325 154 102 4 19 164 9 157 3.97e-12 61.2
MS.gene20731.t1 AT2G21620 30.612 147 85 4 17 163 42 171 1.61e-11 60.5
MS.gene20731.t1 AT2G21620 29.932 147 80 4 17 163 42 165 1.78e-11 60.1
MS.gene20731.t1 AT3G17020 27.815 151 103 2 19 164 11 160 5.17e-11 58.5

Find 29 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAGGTAGTCACGGTTAT+GGG 0.301582 1.2:-66288490 MS.gene20731:CDS
GTTGTAGGTAGTCACGGTTA+TGG 0.361010 1.2:-66288491 MS.gene20731:CDS
CGTGCTTCAATCTTGGTTGT+AGG 0.387207 1.2:-66288506 MS.gene20731:CDS
AAAGTATCGTGCTTCAATCT+TGG 0.402940 1.2:-66288513 MS.gene20731:CDS
ACTTCTTCTGTTGGCCTCGC+TGG 0.403590 1.2:-66289471 MS.gene20731:CDS
ACAGTGCATACGCTCTGAAA+TGG 0.408844 1.2:-66289566 MS.gene20731:CDS
AACAAGAACAAGTTTGAAAA+CGG 0.409228 1.2:+66289512 None:intergenic
TCTGTTGGCCTCGCTGGTCC+TGG 0.423133 1.2:-66289465 MS.gene20731:intron
ACTGGTTGTGATTTCTCTGA+TGG 0.432899 1.2:+66289618 None:intergenic
ACGGTTATGGGGCTATAAAA+AGG 0.451906 1.2:-66288478 MS.gene20731:intron
CTCAAATCAGAGTCCACAAT+TGG 0.452134 1.2:+66289327 None:intergenic
AAGTTTGAAAACGGGATTGT+TGG 0.453772 1.2:+66289521 None:intergenic
CCTGCTGCTACTTCTTCTGT+TGG 0.458113 1.2:-66289480 MS.gene20731:CDS
ACAAGAACAAGTTTGAAAAC+GGG 0.472424 1.2:+66289513 None:intergenic
GAAATCACAACCAGTGATGC+TGG 0.492989 1.2:-66289610 MS.gene20731:CDS
TCGATTGCAACCAGCATCAC+TGG 0.493963 1.2:+66289600 None:intergenic
CAATTGTGGACTCTGATTTG+AGG 0.500278 1.2:-66289326 MS.gene20731:CDS
TCAGGTAAATGATGTAATAG+TGG 0.511125 1.2:-66288582 MS.gene20731:intron
ATCTTGGTTGTAGGTAGTCA+CGG 0.516671 1.2:-66288497 MS.gene20731:CDS
TGCCAGTATAGATCAGTGCT+TGG 0.553700 1.2:+66287924 None:intergenic
TGATGTAATAGTGGAGGTGG+TGG 0.556420 1.2:-66288573 MS.gene20731:CDS
AAATGATGTAATAGTGGAGG+TGG 0.562868 1.2:-66288576 MS.gene20731:CDS
CCAACAGAAGAAGTAGCAGC+AGG 0.584368 1.2:+66289480 None:intergenic
AGTGTACGCAGGAGCTGCTG+AGG 0.594737 1.2:-66289355 MS.gene20731:CDS
TGTAGGTAGTCACGGTTATG+GGG 0.599167 1.2:-66288489 MS.gene20731:CDS
TTGTGCTAATTAGTGTACGC+AGG 0.608881 1.2:-66289366 MS.gene20731:intron
GTAATAGTGGAGGTGGTGGA+AGG 0.626493 1.2:-66288569 MS.gene20731:CDS
GGTAAATGATGTAATAGTGG+AGG 0.637317 1.2:-66288579 MS.gene20731:CDS
GAATTATACCAGGACCAGCG+AGG 0.726201 1.2:+66289457 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATTAAAAAGCAAA+AGG + chr1.2:66289440-66289459 None:intergenic 10.0%
!! ATAAAATTATGAAAAGAATT+AGG + chr1.2:66288429-66288448 None:intergenic 10.0%
!! TAAAATTATGAAAAGAATTA+GGG + chr1.2:66288428-66288447 None:intergenic 10.0%
!!! ATGATATTATTTTAGATAAT+AGG - chr1.2:66288561-66288580 MS.gene20731:CDS 10.0%
!!! TTTCATAATTTTATTTGATA+AGG - chr1.2:66288435-66288454 MS.gene20731:intron 10.0%
!! AAAAATGTTAGAATTAAATG+CGG + chr1.2:66289223-66289242 None:intergenic 15.0%
!!! ACTTTTCATTTTATTTATAC+TGG - chr1.2:66289461-66289480 MS.gene20731:intron 15.0%
!!! ATTACATTTATTCATGTTTT+TGG + chr1.2:66289290-66289309 None:intergenic 15.0%
!!! ATTTTATTTGATAAGGAAAA+TGG - chr1.2:66288442-66288461 MS.gene20731:intron 15.0%
!!! CTTTTCATTTTATTTATACT+GGG - chr1.2:66289462-66289481 MS.gene20731:intron 15.0%
!!! TTTTCATTTTATTTATACTG+GGG - chr1.2:66289463-66289482 MS.gene20731:intron 15.0%
!! AAAATTAAAAAGCAAAAGGA+AGG + chr1.2:66289436-66289455 None:intergenic 20.0%
!! AACAAAAATAGAAACACATA+CGG + chr1.2:66288330-66288349 None:intergenic 20.0%
!! TTAAGTATTTATGTCATATG+TGG - chr1.2:66288947-66288966 MS.gene20731:intron 20.0%
!!! TTTCATTTTATTTATACTGG+GGG - chr1.2:66289464-66289483 MS.gene20731:intron 20.0%
!!! TTTGTTGATGTTTTGATAAT+TGG - chr1.2:66288180-66288199 MS.gene20731:intron 20.0%
! AACAAGAACAAGTTTGAAAA+CGG + chr1.2:66288069-66288088 None:intergenic 25.0%
! ATGAAATTGAGAATTATACC+AGG + chr1.2:66288134-66288153 None:intergenic 25.0%
!!! TGAATTTTCTCTGGTTTATA+CGG - chr1.2:66288477-66288496 MS.gene20731:intron 25.0%
!!! TTAATACACACAAGTTTTGT+TGG + chr1.2:66288866-66288885 None:intergenic 25.0%
!!! TTTTTTGCTAGTTTGATGAA+AGG - chr1.2:66288673-66288692 MS.gene20731:intron 25.0%
AATTAAATGCGGCATATTCA+TGG + chr1.2:66289212-66289231 None:intergenic 30.0%
ACAAGAACAAGTTTGAAAAC+GGG + chr1.2:66288068-66288087 None:intergenic 30.0%
CCTGTATTTATATGTTGTGT+GGG - chr1.2:66288501-66288520 MS.gene20731:CDS 30.0%
TACAATTATCATCCTTCGTT+TGG - chr1.2:66289140-66289159 MS.gene20731:intron 30.0%
TCAGGTAAATGATGTAATAG+TGG - chr1.2:66288996-66289015 MS.gene20731:intron 30.0%
TCCTGTATTTATATGTTGTG+TGG - chr1.2:66288500-66288519 MS.gene20731:CDS 30.0%
TTACCATCATCTTTGTATGT+CGG - chr1.2:66288787-66288806 MS.gene20731:intron 30.0%
! TAAGGAAAATGGCTTTTGAA+TGG - chr1.2:66288453-66288472 MS.gene20731:intron 30.0%
! TAATTTTGTGTTTGTGATCG+TGG + chr1.2:66289262-66289281 None:intergenic 30.0%
! TCTGATAACTGAATTTTCTC+AGG - chr1.2:66288978-66288997 MS.gene20731:intron 30.0%
! TTGAATGGATGAATTTTCTC+TGG - chr1.2:66288468-66288487 MS.gene20731:intron 30.0%
!! TTTTTCTACTTTCGAGTCTA+AGG - chr1.2:66288902-66288921 MS.gene20731:intron 30.0%
!!! CTTTTCTGAAACTTGTGAAT+AGG - chr1.2:66289181-66289200 MS.gene20731:intron 30.0%
AAGTTTGAAAACGGGATTGT+TGG + chr1.2:66288060-66288079 None:intergenic 35.0%
ACCAGTTGAAGCAATTTAAG+TGG + chr1.2:66288757-66288776 None:intergenic 35.0%
AGGTTAAAGATTTCAGTGGA+TGG + chr1.2:66289316-66289335 None:intergenic 35.0%
ATCTTTAACCTGCAGAAGAT+AGG - chr1.2:66289325-66289344 MS.gene20731:CDS 35.0%
CAAAAGGAAGGTTTGATTGT+TGG + chr1.2:66289424-66289443 None:intergenic 35.0%
CCCACACAACATATAAATAC+AGG + chr1.2:66288504-66288523 None:intergenic 35.0%
GGTAAATGATGTAATAGTGG+AGG - chr1.2:66288999-66289018 MS.gene20731:intron 35.0%
TAACCGACATACAAAGATGA+TGG + chr1.2:66288793-66288812 None:intergenic 35.0%
TATGGGGCTATAAAAAGGTA+TGG - chr1.2:66289105-66289124 MS.gene20731:intron 35.0%
TCCACTTAAATTGCTTCAAC+TGG - chr1.2:66288753-66288772 MS.gene20731:intron 35.0%
TTACATATCACTCCAAACGA+AGG + chr1.2:66289155-66289174 None:intergenic 35.0%
TTTATATGTTGTGTGGGACT+TGG - chr1.2:66288507-66288526 MS.gene20731:CDS 35.0%
! AAAAAGGTATGGCTTCATTG+TGG - chr1.2:66289116-66289135 MS.gene20731:intron 35.0%
! AAATGATGTAATAGTGGAGG+TGG - chr1.2:66289002-66289021 MS.gene20731:intron 35.0%
! TCGGTTAATTGTCATGCTTT+TGG - chr1.2:66288806-66288825 MS.gene20731:intron 35.0%
!! AAAGTATCGTGCTTCAATCT+TGG - chr1.2:66289065-66289084 MS.gene20731:intron 35.0%
ACGGTTATGGGGCTATAAAA+AGG - chr1.2:66289100-66289119 MS.gene20731:intron 40.0%
ACTGGTTGTGATTTCTCTGA+TGG + chr1.2:66287963-66287982 None:intergenic 40.0%
ATCTTGGTTGTAGGTAGTCA+CGG - chr1.2:66289081-66289100 MS.gene20731:intron 40.0%
CTCAAATCAGAGTCCACAAT+TGG + chr1.2:66288254-66288273 None:intergenic 40.0%
CTGCAGGTTAAAGATTTCAG+TGG + chr1.2:66289320-66289339 None:intergenic 40.0%
TCGAGTGTAGTGAGTGAATT+AGG + chr1.2:66289541-66289560 None:intergenic 40.0%
TTGTAGGTAGTCACGGTTAT+GGG - chr1.2:66289088-66289107 MS.gene20731:intron 40.0%
TTGTGCTAATTAGTGTACGC+AGG - chr1.2:66288212-66288231 MS.gene20731:intron 40.0%
! ACTCGACTCTTTTAATCTGC+AGG - chr1.2:66289555-66289574 MS.gene20731:CDS 40.0%
! CAATTGTGGACTCTGATTTG+AGG - chr1.2:66288252-66288271 MS.gene20731:intron 40.0%
! CTCGACTCTTTTAATCTGCA+GGG - chr1.2:66289556-66289575 MS.gene20731:CDS 40.0%
! TTTTGTGTTTGTGATCGTGG+TGG + chr1.2:66289259-66289278 None:intergenic 40.0%
AGTTCCATACCCACATCCTT+AGG - chr1.2:66288839-66288858 MS.gene20731:intron 45.0%
CGTGCTTCAATCTTGGTTGT+AGG - chr1.2:66289072-66289091 MS.gene20731:intron 45.0%
GAAATCACAACCAGTGATGC+TGG - chr1.2:66287968-66287987 MS.gene20731:CDS 45.0%
GGGACTTGGAGCATACTATA+TGG - chr1.2:66288521-66288540 MS.gene20731:CDS 45.0%
GTTCCATACCCACATCCTTA+GGG - chr1.2:66288840-66288859 MS.gene20731:intron 45.0%
GTTGTAGGTAGTCACGGTTA+TGG - chr1.2:66289087-66289106 MS.gene20731:intron 45.0%
TAGCTGAATGCATCATGCTC+AGG - chr1.2:66288598-66288617 MS.gene20731:intron 45.0%
TGTAGGTAGTCACGGTTATG+GGG - chr1.2:66289089-66289108 MS.gene20731:intron 45.0%
TTTGTTGGCCCTAAGGATGT+GGG + chr1.2:66288851-66288870 None:intergenic 45.0%
! GACTCTTTTAATCTGCAGGG+CGG - chr1.2:66289559-66289578 MS.gene20731:CDS 45.0%
! TGATGTAATAGTGGAGGTGG+TGG - chr1.2:66289005-66289024 MS.gene20731:intron 45.0%
! TGCTGAGGTTTTGCCAATTG+TGG - chr1.2:66288238-66288257 MS.gene20731:intron 45.0%
! TTAATCTGCAGGGCGGTTTT+AGG - chr1.2:66289566-66289585 MS.gene20731:CDS 45.0%
! TTTTGTTGGCCCTAAGGATG+TGG + chr1.2:66288852-66288871 None:intergenic 45.0%
!! ACAGTGCATACGCTCTGAAA+TGG - chr1.2:66288012-66288031 MS.gene20731:intron 45.0%
!!! CACAAGTTTTGTTGGCCCTA+AGG + chr1.2:66288858-66288877 None:intergenic 45.0%
CCAACAGAAGAAGTAGCAGC+AGG + chr1.2:66288101-66288120 None:intergenic 50.0%
GAATTATACCAGGACCAGCG+AGG + chr1.2:66288124-66288143 None:intergenic 50.0%
TACTGCTGCCTATCTTCTGC+AGG + chr1.2:66289336-66289355 None:intergenic 50.0%
TCGATTGCAACCAGCATCAC+TGG + chr1.2:66287981-66288000 None:intergenic 50.0%
! CCTGCTGCTACTTCTTCTGT+TGG - chr1.2:66288098-66288117 MS.gene20731:intron 50.0%
!! GTAATAGTGGAGGTGGTGGA+AGG - chr1.2:66289009-66289028 MS.gene20731:intron 50.0%
TGGCCCTAAGGATGTGGGTA+TGG + chr1.2:66288846-66288865 None:intergenic 55.0%
! ACTTCTTCTGTTGGCCTCGC+TGG - chr1.2:66288107-66288126 MS.gene20731:intron 55.0%
AGTGTACGCAGGAGCTGCTG+AGG - chr1.2:66288223-66288242 MS.gene20731:intron 60.0%
! TCTGTTGGCCTCGCTGGTCC+TGG - chr1.2:66288113-66288132 MS.gene20731:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 66287936 66289664 66287936 ID=MS.gene20731
chr1.2 mRNA 66287936 66289664 66287936 ID=MS.gene20731.t1;Parent=MS.gene20731
chr1.2 exon 66289466 66289664 66289466 ID=MS.gene20731.t1.exon1;Parent=MS.gene20731.t1
chr1.2 CDS 66289466 66289664 66289466 ID=cds.MS.gene20731.t1;Parent=MS.gene20731.t1
chr1.2 exon 66289272 66289375 66289272 ID=MS.gene20731.t1.exon2;Parent=MS.gene20731.t1
chr1.2 CDS 66289272 66289375 66289272 ID=cds.MS.gene20731.t1;Parent=MS.gene20731.t1
chr1.2 exon 66288479 66288600 66288479 ID=MS.gene20731.t1.exon3;Parent=MS.gene20731.t1
chr1.2 CDS 66288479 66288600 66288479 ID=cds.MS.gene20731.t1;Parent=MS.gene20731.t1
chr1.2 exon 66287936 66288023 66287936 ID=MS.gene20731.t1.exon4;Parent=MS.gene20731.t1
chr1.2 CDS 66287936 66288023 66287936 ID=cds.MS.gene20731.t1;Parent=MS.gene20731.t1
Gene Sequence

>MS.gene20731

ATGGCGAGTGCGAGTTCAGATCAACCATCAGAGAAATCACAACCAGTGATGCTGGTTGCAATCGACGAGAGCGATCACAGTGCATACGCTCTGAAATGGACTCTCGATCATTTCTTCAGCACCAACAATCCCGTTTTCAAACTTGTTCTTGTTCATGCTAGACCTGCTGCTACTTCTTCTGTTGGCCTCGCTGGTCCTGGTATAATTCTCAATTTCATTCTTATTACTAAGTTTTTTTTTTTTTTTTGTTGATGTTTTGATAATTGGATGAACGATTTGTGCTAATTAGTGTACGCAGGAGCTGCTGAGGTTTTGCCAATTGTGGACTCTGATTTGAGGAAGATTGCTGCTAGAGTTGCTGAGAATGCTAAACAACTTTGCATTAAGAAATCCGTATGTGTTTCTATTTTTGTTTTTAATTTTAGATTCTAAAATCTTATTTATATAATAACTTAATTTATCATTGTTTCTTTGTAATTTCATAACGTTCCCTAATTCTTTTCATAATTTTATTTGATAAGGAAAATGGCTTTTGAATGGATGAATTTTCTCTGGTTTATACGGTCCTGTATTTATATGTTGTGTGGGACTTGGAGCATACTATATGGTTTTAGCAAGATTTTCTATGATATTATTTTAGATAATAGGCTGAGAAATATTCATAGCTGAATGCATCATGCTCAGGACTAGACTAGTACACTGCAAATTAAGCAATTTCATAATCTTTGCTAGTTCTGTTTTTTGCTAGTTTGATGAAAGGAGATAAATTGAACGCATTGATGTTAAGATTTATCATGTTTCAATTTCGTGTTGTATTTCCACTTAAATTGCTTCAACTGGTTTAAACAAACTTACCATCATCTTTGTATGTCGGTTAATTGTCATGCTTTTGGAGTGAAATTGAGTTCCATACCCACATCCTTAGGGCCAACAAAACTTGTGTGTATTAATATCATCGTGAATTTATTTTTCTACTTTCGAGTCTAAGGAAAAATGTATACATTATGATGTTTAAGTATTTATGTCATATGTGGAAAAACTCTCTGATAACTGAATTTTCTCAGGTAAATGATGTAATAGTGGAGGTGGTGGAAGGTGATGCTAGAAATGTTCTCTGTGATACAGTAGAAAAGTATCGTGCTTCAATCTTGGTTGTAGGTAGTCACGGTTATGGGGCTATAAAAAGGTATGGCTTCATTGTGGTTACAATTATCATCCTTCGTTTGGAGTGATATGTAATTGTAACTTTTCTGAAACTTGTGAATAGGATATACCATGAATATGCCGCATTTAATTCTAACATTTTTGCTTAAGTGAATGCCACCACGATCACAAACACAAAATTAAATAGCCAAAAACATGAATAAATGTAATTTGCCATCCACTGAAATCTTTAACCTGCAGAAGATAGGCAGCAGTAGTTTTTTGTGATTCACATACAGTTTTCTACATAATTATAAAGTCTCTAGATCATTTCTATTTTAACCAACAATCAAACCTTCCTTTTGCTTTTTAATTTTATTTGACTTTTCATTTTATTTATACTGGGGGAGAACATGTATATACATTGTTGCTTGAAGCAAACACTTCATTTCATAATTTCCTAATTCACTCACTACACTCGACTCTTTTAATCTGCAGGGCGGTTTTAGGTAGTGTGAGTGACTACTGTGCTCATCATGCTCATTGCACTGTGATGATTGTGAAGAAGCCAAAAACCAAGCACTGA
Protein sequence

>MS.gene20731.t1

MASASSDQPSEKSQPVMLVAIDESDHSAYALKWTLDHFFSTNNPVFKLVLVHARPAATSSVGLAGPVYAGAAEVLPIVDSDLRKIAARVAENAKQLCIKKSVNDVIVEVVEGDARNVLCDTVEKYRASILVVGSHGYGAIKRAVLGSVSDYCAHHAHCTVMIVKKPKTKH