Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21015.t1 | XP_013469069.1 | 96.5 | 462 | 9 | 1 | 1 | 455 | 1 | 462 | 4.80E-240 | 840.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21015.t1 | Q9H6T3 | 31.0 | 255 | 135 | 8 | 2 | 215 | 20 | 274 | 4.9e-15 | 84.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21015.t1 | A0A072VMN4 | 96.5 | 462 | 9 | 1 | 1 | 455 | 1 | 462 | 3.5e-240 | 840.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene21015 | MS.gene040499 | PPI |
| MS.gene21015 | MS.gene62607 | PPI |
| MS.gene21015 | MS.gene60613 | PPI |
| MS.gene21015 | MS.gene061126 | PPI |
| MS.gene21015 | MS.gene49154 | PPI |
| MS.gene21015 | MS.gene006231 | PPI |
| MS.gene21015 | MS.gene30654 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21015.t1 | MTR_1g084100 | 96.537 | 462 | 9 | 1 | 1 | 455 | 1 | 462 | 0.0 | 915 |
| MS.gene21015.t1 | MTR_8g107280 | 42.424 | 99 | 56 | 1 | 88 | 185 | 483 | 581 | 1.57e-18 | 88.6 |
| MS.gene21015.t1 | MTR_4g094205 | 44.444 | 99 | 54 | 1 | 88 | 185 | 492 | 590 | 2.13e-17 | 85.1 |
| MS.gene21015.t1 | MTR_3g452710 | 39.286 | 112 | 67 | 1 | 80 | 190 | 7 | 118 | 1.48e-15 | 79.3 |
| MS.gene21015.t1 | MTR_3g452710 | 39.286 | 112 | 67 | 1 | 80 | 190 | 7 | 118 | 2.00e-15 | 78.6 |
| MS.gene21015.t1 | MTR_1g030300 | 37.615 | 109 | 64 | 3 | 84 | 189 | 181 | 288 | 7.73e-12 | 67.4 |
| MS.gene21015.t1 | MTR_3g087480 | 33.929 | 112 | 73 | 1 | 91 | 201 | 463 | 574 | 7.84e-12 | 67.8 |
| MS.gene21015.t1 | MTR_2g078020 | 30.657 | 137 | 88 | 2 | 91 | 226 | 469 | 599 | 9.91e-12 | 67.4 |
| MS.gene21015.t1 | MTR_2g078020 | 30.657 | 137 | 88 | 2 | 91 | 226 | 469 | 599 | 1.06e-11 | 67.4 |
| MS.gene21015.t1 | MTR_3g074230 | 33.628 | 113 | 68 | 2 | 91 | 202 | 431 | 537 | 4.53e-11 | 65.5 |
| MS.gene21015.t1 | MTR_3g074230 | 33.094 | 139 | 70 | 2 | 56 | 171 | 141 | 279 | 5.35e-11 | 65.1 |
| MS.gene21015.t1 | MTR_3g074230 | 33.628 | 113 | 68 | 2 | 91 | 202 | 431 | 537 | 4.78e-11 | 65.5 |
| MS.gene21015.t1 | MTR_3g074230 | 33.094 | 139 | 70 | 2 | 56 | 171 | 141 | 279 | 5.21e-11 | 65.1 |
| MS.gene21015.t1 | MTR_5g012030 | 33.333 | 105 | 69 | 1 | 84 | 187 | 392 | 496 | 8.32e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21015.t1 | AT1G56440 | 51.037 | 482 | 189 | 8 | 2 | 456 | 15 | 476 | 9.19e-151 | 437 |
| MS.gene21015.t1 | AT1G56440 | 50.317 | 473 | 188 | 8 | 11 | 456 | 14 | 466 | 3.58e-143 | 418 |
| MS.gene21015.t1 | AT3G17970 | 44.643 | 112 | 59 | 2 | 75 | 185 | 467 | 576 | 1.27e-20 | 95.1 |
| MS.gene21015.t1 | AT5G09420 | 41.176 | 102 | 59 | 1 | 88 | 188 | 492 | 593 | 3.42e-16 | 81.6 |
| MS.gene21015.t1 | AT2G42810 | 39.286 | 112 | 67 | 1 | 80 | 190 | 9 | 120 | 1.89e-15 | 79.0 |
| MS.gene21015.t1 | AT2G42810 | 39.286 | 112 | 67 | 1 | 80 | 190 | 9 | 120 | 2.05e-15 | 79.0 |
| MS.gene21015.t1 | AT2G42810 | 39.286 | 112 | 67 | 1 | 80 | 190 | 9 | 120 | 3.04e-15 | 78.2 |
| MS.gene21015.t1 | AT2G42810 | 39.286 | 112 | 67 | 1 | 80 | 190 | 9 | 120 | 3.04e-15 | 78.2 |
| MS.gene21015.t1 | AT2G42810 | 39.286 | 112 | 67 | 1 | 80 | 190 | 9 | 120 | 3.04e-15 | 78.2 |
| MS.gene21015.t1 | AT2G42580 | 30.968 | 155 | 101 | 2 | 91 | 239 | 465 | 619 | 1.41e-11 | 67.0 |
| MS.gene21015.t1 | AT3G58620 | 28.994 | 169 | 110 | 3 | 80 | 240 | 445 | 611 | 1.56e-11 | 67.0 |
| MS.gene21015.t1 | AT3G58620 | 28.994 | 169 | 110 | 3 | 80 | 240 | 445 | 611 | 2.65e-11 | 66.2 |
| MS.gene21015.t1 | AT1G12270 | 35.294 | 102 | 65 | 1 | 87 | 187 | 386 | 487 | 4.46e-11 | 65.5 |
| MS.gene21015.t1 | AT4G12400 | 32.331 | 133 | 80 | 3 | 64 | 186 | 340 | 472 | 4.78e-11 | 65.5 |
| MS.gene21015.t1 | AT4G12400 | 32.331 | 133 | 80 | 3 | 64 | 186 | 340 | 472 | 5.14e-11 | 65.1 |
Find 86 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTGGTGATCTTGCTTTGC+AGG | 0.179423 | 1.3:-59844864 | MS.gene21015:CDS |
| TTTCAAACAGAAGAAGTTTA+AGG | 0.181327 | 1.3:-59849308 | MS.gene21015:CDS |
| GTAACGATCATCGAGATTTA+AGG | 0.189585 | 1.3:+59848954 | None:intergenic |
| CTTGCCTTCTTAGCTTTATA+TGG | 0.195569 | 1.3:+59849212 | None:intergenic |
| GATGGAGTATGCTGAGATTT+TGG | 0.222982 | 1.3:-59843797 | MS.gene21015:CDS |
| TTTGCAGGCTTGTCTGTTAA+AGG | 0.263046 | 1.3:-59844849 | MS.gene21015:intron |
| TTCCTTATGTGACTTGGTTT+TGG | 0.263836 | 1.3:+59850740 | None:intergenic |
| CTGCTGTAGCCTATGCAAAT+AGG | 0.267162 | 1.3:-59849243 | MS.gene21015:CDS |
| TTCTTGACTTCTTGATTGTT+AGG | 0.278082 | 1.3:+59848737 | None:intergenic |
| GGATCTAGTTGTTAGTTATA+TGG | 0.278767 | 1.3:-59844524 | MS.gene21015:CDS |
| TACAGTGTTTCTTAGTGCTT+TGG | 0.287972 | 1.3:+59845664 | None:intergenic |
| GGGTTTCTTGAACGACTTGC+AGG | 0.331237 | 1.3:-59850793 | MS.gene21015:CDS |
| TGCTAAAGAACCACTCTTGA+TGG | 0.363324 | 1.3:-59845372 | MS.gene21015:CDS |
| AAGTCTGAGACAAAAGTTAA+TGG | 0.365292 | 1.3:-59845629 | MS.gene21015:CDS |
| CTTATCAGTTTGAGGTCTCT+TGG | 0.365519 | 1.3:-59844895 | MS.gene21015:CDS |
| TGCTGTAGCCTATGCAAATA+GGG | 0.377706 | 1.3:-59849242 | MS.gene21015:CDS |
| CTGCAGATTTGCGCAAGATC+TGG | 0.380066 | 1.3:-59843849 | MS.gene21015:intron |
| CTTGAACGACTTGCAGGATT+GGG | 0.392289 | 1.3:-59850787 | MS.gene21015:CDS |
| ACTAGTAGGAAATGGTTCTA+AGG | 0.403610 | 1.3:-59845303 | MS.gene21015:CDS |
| TGAAGCTCATCGTCAAATAA+AGG | 0.403993 | 1.3:-59845555 | MS.gene21015:intron |
| TCATAAAGGGTTCATCTTCT+CGG | 0.408846 | 1.3:-59849760 | MS.gene21015:CDS |
| TTGCCTTCTTAGCTTTATAT+GGG | 0.409244 | 1.3:+59849213 | None:intergenic |
| AACTACAGCTTATCAGTTTG+AGG | 0.411492 | 1.3:-59844903 | MS.gene21015:CDS |
| ATCAAGAGTGGTTCTTTAGC+AGG | 0.431104 | 1.3:+59845374 | None:intergenic |
| TGCAAGCCACAGCTTGGAAC+AGG | 0.445688 | 1.3:-59845270 | MS.gene21015:intron |
| TCTTGAACGACTTGCAGGAT+TGG | 0.448057 | 1.3:-59850788 | MS.gene21015:CDS |
| AAGCTATTGATTGCTATTCA+AGG | 0.460436 | 1.3:-59849285 | MS.gene21015:CDS |
| ACTCAAGGACTAGTAGGAAA+TGG | 0.489220 | 1.3:-59845311 | MS.gene21015:CDS |
| TCTTTCTCAGAAGCAGCATC+AGG | 0.489375 | 1.3:+59849611 | None:intergenic |
| GTCCAAAACCAAGTCACATA+AGG | 0.490128 | 1.3:-59850742 | MS.gene21015:CDS |
| AACTAGCAGGGTAGAAGATA+AGG | 0.492686 | 1.3:+59844671 | None:intergenic |
| AGTTTGAGGTCTCTTGGAGA+GGG | 0.494603 | 1.3:-59844889 | MS.gene21015:CDS |
| GTTCTTTGCAGCTTCAGCCA+TGG | 0.504037 | 1.3:+59844937 | None:intergenic |
| TCTCGGAGTGATGGATCAAA+AGG | 0.505677 | 1.3:-59849743 | MS.gene21015:CDS |
| GTGCCTTCCATCAGCTGCTA+AGG | 0.506136 | 1.3:-59844461 | MS.gene21015:CDS |
| TGCCGAGTTTGCCATGAGGT+TGG | 0.507363 | 1.3:-59848762 | MS.gene21015:CDS |
| CTGCGCTGAGGCAACTCCGA+TGG | 0.507557 | 1.3:-59843815 | MS.gene21015:CDS |
| CAGTTTGAGGTCTCTTGGAG+AGG | 0.513770 | 1.3:-59844890 | MS.gene21015:CDS |
| GATGGAGGAGATCACTAGTA+AGG | 0.515376 | 1.3:-59845354 | MS.gene21015:CDS |
| CACATCAAATCTCGGTACCT+TGG | 0.515743 | 1.3:+59844492 | None:intergenic |
| ACTTGATGATCTCAACTAGC+AGG | 0.517918 | 1.3:+59844658 | None:intergenic |
| TCAAGCTGCTTCTCGAGCCA+TGG | 0.518342 | 1.3:-59844954 | MS.gene21015:CDS |
| ATTGTTAGGTTCCAACCTCA+TGG | 0.526662 | 1.3:+59848751 | None:intergenic |
| TGGCCCATATAAAGCTAAGA+AGG | 0.528942 | 1.3:-59849216 | MS.gene21015:intron |
| GAGTCGTGTGAGTAGCAGTT+TGG | 0.530179 | 1.3:-59849648 | MS.gene21015:CDS |
| AGCATACTCTCGGCGAGCAA+CGG | 0.533228 | 1.3:-59848926 | MS.gene21015:CDS |
| GGTTCATCTTCTCGGAGTGA+TGG | 0.534108 | 1.3:-59849752 | MS.gene21015:CDS |
| AACTGATAAGCTGTAGTTGG+TGG | 0.540121 | 1.3:+59844908 | None:intergenic |
| ATACCATCCTTAGCAGCTGA+TGG | 0.542897 | 1.3:+59844454 | None:intergenic |
| GGCTACAGCAGTGGGTGAGA+AGG | 0.545729 | 1.3:+59849255 | None:intergenic |
| ATTTGACGATGAGCTTCAGT+TGG | 0.547813 | 1.3:+59845560 | None:intergenic |
| TTCCAACCTCATGGCAAACT+CGG | 0.550264 | 1.3:+59848760 | None:intergenic |
| AGCAGGACTCAAGGACTAGT+AGG | 0.551356 | 1.3:-59845317 | MS.gene21015:CDS |
| CTCCGATCAAAATTCTACCT+TGG | 0.554742 | 1.3:-59843769 | MS.gene21015:CDS |
| CAAGTGAGTGTCACTGTCCA+AGG | 0.558456 | 1.3:+59843752 | None:intergenic |
| CAGCAGATTTCAAGAGGCTG+AGG | 0.559168 | 1.3:-59848992 | MS.gene21015:intron |
| TCAAACTGATAAGCTGTAGT+TGG | 0.559212 | 1.3:+59844905 | None:intergenic |
| GTAGGAAATGGTTCTAAGGA+AGG | 0.565761 | 1.3:-59845299 | MS.gene21015:CDS |
| TTATAATGCAAGCCACAGCT+TGG | 0.565861 | 1.3:-59845276 | MS.gene21015:CDS |
| AGGATACAAAATCTGCAAGC+AGG | 0.568821 | 1.3:-59845334 | MS.gene21015:CDS |
| GCTTAACCTGTTCCAAGCTG+TGG | 0.570744 | 1.3:+59845264 | None:intergenic |
| CATCCTTAGCAGCTGATGGA+AGG | 0.574291 | 1.3:+59844458 | None:intergenic |
| AAATCTGCAAGCAGGACTCA+AGG | 0.576402 | 1.3:-59845326 | MS.gene21015:CDS |
| GCAACGGCTAGAAAAGAACT+TGG | 0.576648 | 1.3:-59848910 | MS.gene21015:CDS |
| CTTCCATCAGCTGCTAAGGA+TGG | 0.577022 | 1.3:-59844457 | MS.gene21015:intron |
| GGCCATTGCCCTATTTGCAT+AGG | 0.577379 | 1.3:+59849234 | None:intergenic |
| CTCGTACAGTGACTTAGCAT+CGG | 0.584635 | 1.3:+59848706 | None:intergenic |
| ATTACGATCACATCAAATCT+CGG | 0.593584 | 1.3:+59844484 | None:intergenic |
| GAGAAATCATAAAACTAAGA+CGG | 0.599841 | 1.3:-59845008 | MS.gene21015:CDS |
| ATGTGTCAGCAGATTTCAAG+AGG | 0.608715 | 1.3:-59848998 | MS.gene21015:intron |
| TTTGCATAGGCTACAGCAGT+GGG | 0.613483 | 1.3:+59849247 | None:intergenic |
| GTTACATAAAAGCATACTCT+CGG | 0.615745 | 1.3:-59848936 | MS.gene21015:CDS |
| AGCCTATGCAAATAGGGCAA+TGG | 0.619385 | 1.3:-59849236 | MS.gene21015:CDS |
| AAAATCTCAGCATACTCCAT+CGG | 0.624335 | 1.3:+59843799 | None:intergenic |
| TGCTGCTTCTGAGAAAGACC+TGG | 0.631182 | 1.3:-59849606 | MS.gene21015:intron |
| GGATTGGGAACTTTCTACCA+AGG | 0.633244 | 1.3:-59850772 | MS.gene21015:CDS |
| TGCTAAGTCACTGTACGAGA+AGG | 0.634474 | 1.3:-59848702 | MS.gene21015:intron |
| TAGTACTATGTGAAACAGAG+TGG | 0.636916 | 1.3:+59845594 | None:intergenic |
| CTTGATGATCTCAACTAGCA+GGG | 0.642984 | 1.3:+59844659 | None:intergenic |
| ATTTGCATAGGCTACAGCAG+TGG | 0.647481 | 1.3:+59849246 | None:intergenic |
| GTGATCTCCTCCATCAAGAG+TGG | 0.650649 | 1.3:+59845362 | None:intergenic |
| TTATATGGATAATTTAACCA+AGG | 0.659529 | 1.3:-59844509 | MS.gene21015:CDS |
| TAAAGAACCACTCTTGATGG+AGG | 0.671168 | 1.3:-59845369 | MS.gene21015:CDS |
| GGCTGAGGATGACTGTACAG+AGG | 0.702052 | 1.3:-59848977 | MS.gene21015:CDS |
| CAGATGCCGAGTTTGCCATG+AGG | 0.707223 | 1.3:-59848766 | MS.gene21015:CDS |
| ATACTTGTCAGGGTAATGAG+CGG | 0.714762 | 1.3:-59845403 | MS.gene21015:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 59843762 | 59850938 | 59843762 | ID=MS.gene21015 |
| chr1.3 | mRNA | 59843762 | 59850938 | 59843762 | ID=MS.gene21015.t1;Parent=MS.gene21015 |
| chr1.3 | exon | 59850936 | 59850938 | 59850936 | ID=MS.gene21015.t1.exon1;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59850936 | 59850938 | 59850936 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59850737 | 59850823 | 59850737 | ID=MS.gene21015.t1.exon2;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59850737 | 59850823 | 59850737 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59849607 | 59849786 | 59849607 | ID=MS.gene21015.t1.exon3;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59849607 | 59849786 | 59849607 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59849217 | 59849341 | 59849217 | ID=MS.gene21015.t1.exon4;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59849217 | 59849341 | 59849217 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59848887 | 59849008 | 59848887 | ID=MS.gene21015.t1.exon5;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59848887 | 59849008 | 59848887 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59848703 | 59848785 | 59848703 | ID=MS.gene21015.t1.exon6;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59848703 | 59848785 | 59848703 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59845556 | 59845705 | 59845556 | ID=MS.gene21015.t1.exon7;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59845556 | 59845705 | 59845556 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59845271 | 59845414 | 59845271 | ID=MS.gene21015.t1.exon8;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59845271 | 59845414 | 59845271 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59844850 | 59845029 | 59844850 | ID=MS.gene21015.t1.exon9;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59844850 | 59845029 | 59844850 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59844639 | 59844733 | 59844639 | ID=MS.gene21015.t1.exon10;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59844639 | 59844733 | 59844639 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59844458 | 59844555 | 59844458 | ID=MS.gene21015.t1.exon11;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59844458 | 59844555 | 59844458 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| chr1.3 | exon | 59843762 | 59843865 | 59843762 | ID=MS.gene21015.t1.exon12;Parent=MS.gene21015.t1 |
| chr1.3 | CDS | 59843762 | 59843865 | 59843762 | ID=cds.MS.gene21015.t1;Parent=MS.gene21015.t1 |
| Gene Sequence |
| Protein sequence |
