AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21627

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21627.t1 MTR_5g009780 95.143 803 39 0 1 803 1 803 0.0 1536
MS.gene21627.t1 MTR_5g009800 91.562 794 46 2 1 794 1 773 0.0 1460
MS.gene21627.t1 MTR_5g009770 88.903 802 85 2 1 800 1 800 0.0 1453
MS.gene21627.t1 MTR_5g009770 87.779 671 78 2 132 800 1 669 0.0 1204
MS.gene21627.t1 MTR_4g078120 60.714 812 297 6 6 800 3 809 0.0 961
MS.gene21627.t1 MTR_4g123583 59.446 794 306 5 6 786 3 793 0.0 931
MS.gene21627.t1 MTR_8g093780 47.222 792 397 12 4 779 5 791 0.0 717
MS.gene21627.t1 MTR_3g096380 45.859 809 420 8 10 803 13 818 0.0 706
MS.gene21627.t1 MTR_2g011520 44.720 767 414 7 18 779 3 764 0.0 674
MS.gene21627.t1 MTR_7g099800 49.938 811 357 16 15 779 8 815 0.0 671
MS.gene21627.t1 MTR_7g099820 47.342 790 382 13 16 780 5 785 0.0 655
MS.gene21627.t1 MTR_7g099750 48.846 823 341 17 15 779 8 808 0.0 650
MS.gene21627.t1 MTR_7g099820 48.924 697 331 9 16 694 5 694 0.0 624
MS.gene21627.t1 MTR_3g031170 34.319 778 475 11 10 778 28 778 3.26e-139 431
MS.gene21627.t1 MTR_2g040100 33.660 817 489 22 22 802 35 834 2.00e-132 414
MS.gene21627.t1 MTR_3g071990 32.184 783 497 13 6 778 7 765 6.10e-128 402
MS.gene21627.t1 MTR_1g043400 32.290 799 486 20 18 782 11 788 2.92e-123 390
MS.gene21627.t1 MTR_1g045870 32.075 795 478 19 18 782 11 773 7.44e-121 383
MS.gene21627.t1 MTR_0002s1180 31.205 830 507 19 8 798 2 806 6.33e-119 378
MS.gene21627.t1 MTR_5g067690 31.778 793 492 16 19 782 23 795 1.93e-118 377
MS.gene21627.t1 MTR_5g074340 30.946 782 491 17 18 778 30 783 1.98e-117 375
MS.gene21627.t1 MTR_5g074380 31.964 779 483 16 19 778 76 826 1.99e-117 375
MS.gene21627.t1 MTR_5g074360 31.443 776 491 15 19 778 31 781 1.17e-115 370
MS.gene21627.t1 MTR_3g071990 32.336 702 441 13 87 778 6 683 3.12e-113 361
MS.gene21627.t1 MTR_3g037270 30.991 797 506 19 9 782 17 792 3.96e-110 355
MS.gene21627.t1 MTR_3g071870 30.000 780 493 16 18 767 32 788 7.87e-110 355
MS.gene21627.t1 MTR_5g074320 32.000 725 456 14 68 778 10 711 1.69e-109 352
MS.gene21627.t1 MTR_3g071880 29.658 789 509 20 18 777 19 790 2.61e-109 353
MS.gene21627.t1 MTR_3g037260 29.639 803 530 16 22 801 20 810 1.02e-104 341
MS.gene21627.t1 MTR_3g031160 28.553 788 519 17 10 781 23 782 1.65e-102 334
MS.gene21627.t1 MTR_6g015940 30.087 801 500 20 4 778 19 785 3.83e-96 317
MS.gene21627.t1 MTR_4g037755 29.542 765 497 20 49 790 35 780 3.45e-95 315
MS.gene21627.t1 MTR_8g085260 28.892 803 515 18 1 778 18 789 5.96e-94 311
MS.gene21627.t1 MTR_4g029520 27.756 771 515 17 38 781 25 780 8.02e-93 308
MS.gene21627.t1 MTR_3g498715 28.518 803 491 20 9 774 32 788 1.93e-92 308
MS.gene21627.t1 MTR_6g053320 29.741 733 458 20 81 785 69 772 3.74e-92 306
MS.gene21627.t1 MTR_5g088470 27.970 808 513 23 4 778 17 788 3.52e-91 304
MS.gene21627.t1 MTR_5g088450 28.215 801 523 18 4 778 17 791 3.10e-89 299
MS.gene21627.t1 MTR_3g110700 27.023 766 527 13 38 781 22 777 5.06e-89 298
MS.gene21627.t1 MTR_3g083850 27.385 807 515 19 4 778 17 784 9.38e-88 295
MS.gene21627.t1 MTR_7g085870 27.708 794 501 20 25 778 34 794 1.99e-86 292
MS.gene21627.t1 MTR_5g088430 27.869 793 512 19 14 778 28 788 3.92e-86 291
MS.gene21627.t1 MTR_3g031030 28.031 767 480 19 49 779 35 765 8.28e-85 286
MS.gene21627.t1 MTR_5g088790 28.250 800 520 19 9 778 39 814 9.29e-80 274
MS.gene21627.t1 MTR_5g088840 27.826 805 518 20 9 778 39 815 9.10e-79 271
MS.gene21627.t1 MTR_7g052680 28.020 803 518 22 9 778 39 814 6.77e-78 268
MS.gene21627.t1 MTR_7g053000 27.409 799 528 18 9 778 39 814 9.16e-78 268
MS.gene21627.t1 MTR_7g052970 27.409 799 528 18 9 778 39 814 7.89e-77 266
MS.gene21627.t1 MTR_3g465680 28.329 413 286 4 20 426 44 452 5.69e-51 193
MS.gene21627.t1 MTR_3g465680 30.412 388 233 12 401 778 555 915 7.48e-40 159
MS.gene21627.t1 MTR_7g052895 23.400 547 391 10 9 534 39 578 3.73e-41 162
MS.gene21627.t1 MTR_5g083560 32.258 341 192 10 469 796 1 315 6.70e-39 148
MS.gene21627.t1 MTR_5g061460 56.250 96 42 0 455 550 15 110 2.80e-31 120
MS.gene21627.t1 MTR_1g082340 56.250 96 42 0 455 550 82 177 5.76e-31 122
MS.gene21627.t1 MTR_3g083860 25.000 236 135 7 14 244 28 226 1.50e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21627.t1 AT5G41610 66.961 793 251 7 1 785 1 790 0.0 1049
MS.gene21627.t1 AT5G41610 66.961 793 251 7 1 785 1 790 0.0 1049
MS.gene21627.t1 AT4G23700 61.200 817 297 7 6 802 3 819 0.0 974
MS.gene21627.t1 AT4G23700 61.200 817 297 7 6 802 3 819 0.0 974
MS.gene21627.t1 AT5G41610 66.943 723 230 6 71 785 1 722 0.0 949
MS.gene21627.t1 AT3G17630 60.618 777 300 3 3 779 2 772 0.0 932
MS.gene21627.t1 AT3G17630 60.618 777 300 3 3 779 2 772 0.0 932
MS.gene21627.t1 AT1G64170 56.258 791 312 14 11 779 14 792 0.0 824
MS.gene21627.t1 AT2G13620 47.744 798 379 10 4 779 8 789 0.0 716
MS.gene21627.t1 AT3G53720 50.616 812 354 14 15 781 8 817 0.0 693
MS.gene21627.t1 AT3G53720 48.993 745 333 14 82 781 2 744 0.0 598
MS.gene21627.t1 AT1G05580 34.585 772 477 12 23 779 41 799 3.94e-137 427
MS.gene21627.t1 AT2G31910 33.420 769 490 10 24 779 28 787 3.35e-125 395
MS.gene21627.t1 AT1G05580 34.857 700 428 12 95 779 2 688 3.13e-124 390
MS.gene21627.t1 AT1G06970 31.194 779 505 15 10 778 27 784 1.11e-113 365
MS.gene21627.t1 AT2G31910 33.381 698 443 10 95 779 2 690 1.44e-110 354
MS.gene21627.t1 AT2G31910 33.381 698 443 10 95 779 2 690 1.44e-110 354
MS.gene21627.t1 AT2G30240 30.100 804 528 15 10 800 29 811 8.33e-105 342
MS.gene21627.t1 AT5G58460 27.869 793 519 14 21 778 53 827 1.18e-92 310
MS.gene21627.t1 AT5G37060 27.330 794 524 15 21 778 53 829 5.55e-90 302
MS.gene21627.t1 AT5G22900 26.838 816 521 18 14 778 27 817 2.73e-86 291
MS.gene21627.t1 AT5G01690 27.134 785 523 17 10 778 16 767 1.97e-84 285
MS.gene21627.t1 AT3G44900 27.150 814 527 19 14 783 26 817 1.29e-79 273
MS.gene21627.t1 AT3G52080 27.053 828 507 21 3 778 2 784 6.81e-79 271
MS.gene21627.t1 AT2G28180 27.910 799 517 19 12 778 37 808 2.81e-76 264
MS.gene21627.t1 AT1G08150 27.771 785 493 22 24 772 56 802 3.69e-74 258
MS.gene21627.t1 AT5G01680 26.438 817 516 23 1 778 5 775 4.27e-73 254
MS.gene21627.t1 AT1G08140 27.527 752 484 23 57 778 83 803 1.25e-72 254
MS.gene21627.t1 AT1G79400 26.554 772 540 13 24 778 11 772 5.64e-71 249
MS.gene21627.t1 AT2G28170 26.564 783 521 22 19 778 47 798 1.87e-66 236
MS.gene21627.t1 AT5G22910 26.864 778 490 18 31 768 47 785 6.06e-66 235
MS.gene21627.t1 AT1G16380 24.685 794 535 19 33 790 19 785 8.77e-65 231
MS.gene21627.t1 AT3G44930 25.819 763 514 16 31 769 35 769 3.65e-64 229
MS.gene21627.t1 AT3G44910 24.125 800 529 17 1 767 1 755 2.59e-61 221
MS.gene21627.t1 AT3G44920 24.903 771 525 16 31 776 35 776 5.31e-60 218
MS.gene21627.t1 AT1G08135 25.176 711 462 20 95 778 127 794 2.03e-51 193

Find 187 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATCTTCTAAAACCTTTA+AGG 0.099756 5.2:-3464174 MS.gene21627:CDS
TTCCGCATTTCTTGTATATA+TGG 0.132317 5.2:-3463339 MS.gene21627:CDS
GGTGAATGTTCCTCCATTTC+TGG 0.139824 5.2:+3461375 None:intergenic
ACCTCTCACAAAATTCTTTA+AGG 0.140574 5.2:+3462270 None:intergenic
TGATCAGACCTTTGCTATTA+TGG 0.150282 5.2:-3462528 MS.gene21627:CDS
GCTCTTGCTGTTGCCTTATC+AGG 0.189626 5.2:-3463182 MS.gene21627:CDS
GACAAAATGGGTTGCCGTTT+TGG 0.198490 5.2:-3462217 MS.gene21627:CDS
TCCTGTATTAGCCCGTATTT+TGG 0.201280 5.2:-3463291 MS.gene21627:CDS
ATGACTCGAGGTTGCCTTAA+AGG 0.205612 5.2:+3464160 None:intergenic
GTGATCTGTCCTACAATATT+AGG 0.206047 5.2:+3461747 None:intergenic
GTTACATTTACGGCTTGTTT+TGG 0.223359 5.2:-3462768 MS.gene21627:CDS
GCACAAAAGGTAAGACAAAA+TGG 0.243930 5.2:-3462230 MS.gene21627:CDS
ATTTCTGGGACAAAGTTTAT+TGG 0.255303 5.2:+3461390 None:intergenic
CCGGCAAACGCTCCATCTTT+TGG 0.270031 5.2:+3462918 None:intergenic
TTAGCTGCTGTTCTGGCTGC+TGG 0.279693 5.2:-3463005 MS.gene21627:CDS
CCGATGCAGCCTGCGTCTAA+TGG 0.279802 5.2:-3464286 MS.gene21627:CDS
TGGGCCATCCATTTGAAAAT+TGG 0.287723 5.2:+3463077 None:intergenic
TGCTACATTAGCTGCTGTTC+TGG 0.289882 5.2:-3463012 MS.gene21627:CDS
GACCGTGAAGCTCTTGCTTA+TGG 0.299902 5.2:-3461828 MS.gene21627:CDS
CCGCACAACAGCATCTGTTT+TGG 0.302658 5.2:-3461439 MS.gene21627:CDS
CCTAAATCTCTGCGGAAAAC+TGG 0.313847 5.2:-3463467 MS.gene21627:CDS
AGAAGCGAGTGCGCGGAATT+AGG 0.315072 5.2:-3461495 MS.gene21627:CDS
GACCAACATAGCTACCATTC+AGG 0.327011 5.2:-3462820 MS.gene21627:CDS
ATCGGAATTCATGCCATGTT+TGG 0.328149 5.2:-3462966 MS.gene21627:CDS
GTCCTTGCCATTGCTATTGC+CGG 0.332317 5.2:-3463437 MS.gene21627:CDS
TCCGCATTTCTTGTATATAT+GGG 0.333877 5.2:-3463338 MS.gene21627:CDS
CCATTCAGGGGCTGCAGTCA+TGG 0.338451 5.2:-3462806 MS.gene21627:CDS
TTACCCTTTGCACTAGGAAT+TGG 0.338535 5.2:-3463407 MS.gene21627:CDS
TTATTGGAACCTAATATTGT+AGG 0.354215 5.2:-3461756 MS.gene21627:CDS
AACCTTTGTTGCTTATGAAA+TGG 0.354534 5.2:+3462026 None:intergenic
TGGTTCATCGTTTGTTCTAC+AGG 0.363937 5.2:-3463387 MS.gene21627:CDS
TGCGGAAGTATTTACACCTT+TGG 0.366289 5.2:+3463355 None:intergenic
GCTCACCTTCATGGCATTGT+TGG 0.366554 5.2:+3463057 None:intergenic
AAAATAGAGGATCTTGTATC+TGG 0.375448 5.2:-3462882 MS.gene21627:CDS
CTCACCTTCATGGCATTGTT+GGG 0.377633 5.2:+3463058 None:intergenic
ATCTCCATTTGTGTCATTGT+GGG 0.381610 5.2:-3463150 MS.gene21627:CDS
CATCAACCGGCTCACCTTCA+TGG 0.381935 5.2:+3463048 None:intergenic
CATTCAGGGGCTGCAGTCAT+GGG 0.383910 5.2:-3462805 MS.gene21627:CDS
TTCTGCAACTTAATTCTTGT+GGG 0.384157 5.2:-3461555 MS.gene21627:CDS
AACATTTCGAGCTGTGGTAT+TGG 0.387586 5.2:+3464318 None:intergenic
ATTGATGAAATTGCTATCAT+TGG 0.392049 5.2:+3462113 None:intergenic
CCAAAAGATGGAGCGTTTGC+CGG 0.392899 5.2:-3462918 MS.gene21627:CDS
TAATCTTATGCCAGGGTGTT+CGG 0.399773 5.2:+3461794 None:intergenic
AATAAACTTTGTCCCAGAAA+TGG 0.400832 5.2:-3461388 MS.gene21627:CDS
AGGATTCTTGCCTGTTTCCA+TGG 0.404187 5.2:-3462380 MS.gene21627:CDS
GAAAAGCACAACAATGCAAT+AGG 0.404326 5.2:+3461866 None:intergenic
ATCTAATACTGGAAGACTCT+TGG 0.407831 5.2:+3463547 None:intergenic
TCGAAGGCCGGACGGTGAAT+TGG 0.408471 5.2:-3461532 MS.gene21627:CDS
CCATGTTATTGTGGCGTTCG+AGG 0.411384 5.2:-3462168 MS.gene21627:CDS
TTGCCTGTTTCCATGGTTCA+AGG 0.413150 5.2:-3462373 MS.gene21627:CDS
GTGAATGTTCCTCCATTTCT+GGG 0.415037 5.2:+3461376 None:intergenic
TGCAAAGGGTAAGCTTATTC+CGG 0.415247 5.2:+3463418 None:intergenic
CTACTTGGGCCGTCAGCGCT+TGG 0.415516 5.2:-3463605 MS.gene21627:CDS
ATTCCTTGAACCATGGAAAC+AGG 0.420826 5.2:+3462370 None:intergenic
TTTGCTAGAGTATCTAATAC+TGG 0.434840 5.2:+3463536 None:intergenic
GACGCAGGCTGCATCGGCGG+CGG 0.445153 5.2:+3464292 None:intergenic
TCTAATACTGGAAGACTCTT+GGG 0.445615 5.2:+3463548 None:intergenic
CCGCATTTCTTGTATATATG+GGG 0.447740 5.2:-3463337 MS.gene21627:CDS
GCCAAAATACGGGCTAATAC+AGG 0.448964 5.2:+3463290 None:intergenic
ATCGATACTAATGGCACAAA+AGG 0.453823 5.2:-3462243 MS.gene21627:CDS
GCGTCTAATGGTGTATTTCA+AGG 0.458325 5.2:-3464274 MS.gene21627:CDS
AATTCTTGTGGGTCGAAGGC+CGG 0.459743 5.2:-3461544 MS.gene21627:CDS
ACACCCTGGCATAAGATTAG+TGG 0.462991 5.2:-3461790 MS.gene21627:CDS
TAGCTGCTGTTCTGGCTGCT+GGG 0.463429 5.2:-3463004 MS.gene21627:CDS
CCCCTGAATGGTAGCTATGT+TGG 0.463640 5.2:+3462818 None:intergenic
TTGTGTTAGCTGCATATAAG+AGG 0.463707 5.2:-3462466 MS.gene21627:CDS
TTTCTGCAACTTAATTCTTG+TGG 0.464281 5.2:-3461556 MS.gene21627:CDS
ACGCAGGCTGCATCGGCGGC+GGG 0.465545 5.2:+3464293 None:intergenic
ATCTGATACTGAATTACTGC+TGG 0.466334 5.2:+3461701 None:intergenic
TTTGATGAATAGTAAAGGCT+TGG 0.466519 5.2:-3462673 MS.gene21627:CDS
TCATTGTGGGTCTTCTTAAG+TGG 0.468755 5.2:-3463137 MS.gene21627:CDS
ATCGATGATAAACTTGATTG+AGG 0.470338 5.2:-3462342 MS.gene21627:CDS
TTGAACGAGTCTTTAGTGCT+TGG 0.470752 5.2:-3462699 MS.gene21627:CDS
TCTTGCAGTCACAAGGGGAC+TGG 0.473223 5.2:-3464197 MS.gene21627:CDS
TTTGTTGATCGCGGGCTTGG+TGG 0.473988 5.2:-3461918 MS.gene21627:CDS
AAGACCCACAATGACACAAA+TGG 0.479162 5.2:+3463146 None:intergenic
TGACAGGGAGGAATCCTACT+TGG 0.484231 5.2:-3463620 MS.gene21627:intron
AATGATAGCAATTTCATCAA+TGG 0.488394 5.2:-3462111 MS.gene21627:CDS
AGGACCTGTTGGTGGCTTGT+TGG 0.488446 5.2:-3461475 MS.gene21627:CDS
GTCTTGTTCTTGCAGTCACA+AGG 0.489013 5.2:-3464204 MS.gene21627:CDS
GAAGATTCCGTGTCTCCATC+AGG 0.489657 5.2:+3461354 None:intergenic
CTCCATAAGCAAGAGCTTCA+CGG 0.491122 5.2:+3461826 None:intergenic
CATATGTATAACTCATCAAC+CGG 0.492318 5.2:+3463035 None:intergenic
AGATTGGCTATTGCCTGATA+AGG 0.492432 5.2:+3463169 None:intergenic
CAACATCGGTAAAGATAGAA+AGG 0.492937 5.2:-3462634 MS.gene21627:intron
CCATGACTGCAGCCCCTGAA+TGG 0.495906 5.2:+3462806 None:intergenic
CCACTTGACACAAAATAAAG+TGG 0.501014 5.2:+3462849 None:intergenic
AGAGTCTCCAACATCAACTT+TGG 0.503014 5.2:+3461725 None:intergenic
GCAAAGGCCAATTCACCGTC+CGG 0.504951 5.2:+3461525 None:intergenic
GATGCCAACAAGCCACCAAC+AGG 0.505963 5.2:+3461471 None:intergenic
AATCTCCATTTGTGTCATTG+TGG 0.506174 5.2:-3463151 MS.gene21627:CDS
TGGGTCTTCTTAAGTGGGTG+TGG 0.508408 5.2:-3463131 MS.gene21627:CDS
GAACCAATTCCTAGTGCAAA+GGG 0.508620 5.2:+3463404 None:intergenic
TTCTATCTTTACCGATGTTG+AGG 0.509100 5.2:+3462637 None:intergenic
TCAATCAAGTTTATCATCGA+TGG 0.509658 5.2:+3462344 None:intergenic
CATAAGCAACAAAGGTTAGA+CGG 0.512409 5.2:-3462020 MS.gene21627:CDS
TCTTGATTATGCACTTCCAT+TGG 0.513464 5.2:-3464242 MS.gene21627:CDS
AAAACCACTAATCTTATGCC+AGG 0.514861 5.2:+3461786 None:intergenic
CATTGTGGGTCTTCTTAAGT+GGG 0.515396 5.2:-3463136 MS.gene21627:CDS
AGCAAGAGCAAGAAGAATCC+AGG 0.517879 5.2:+3463196 None:intergenic
CACAAAAGGTAAGACAAAAT+GGG 0.518007 5.2:-3462229 MS.gene21627:CDS
TTATTCCGTCCAAGCGCTGA+CGG 0.519020 5.2:+3463596 None:intergenic
CTCGCTTCTTTCAAGAGCAA+AGG 0.521580 5.2:+3461509 None:intergenic
ACAATGACACAAATGGAGAT+TGG 0.521930 5.2:+3463153 None:intergenic
CCATTAGACGCAGGCTGCAT+CGG 0.522930 5.2:+3464286 None:intergenic
TCTTGCTTATGGAGCTCGTA+TGG 0.526592 5.2:-3461817 MS.gene21627:CDS
TTCCATTTCATAAGCAACAA+AGG 0.530602 5.2:-3462028 MS.gene21627:CDS
ACTTAATTCTTGTGGGTCGA+AGG 0.531650 5.2:-3461548 MS.gene21627:CDS
TTGGGCCGTCAGCGCTTGGA+CGG 0.534153 5.2:-3463601 MS.gene21627:CDS
GGGTTTGTCACGGATGCTAT+CGG 0.536342 5.2:-3462984 MS.gene21627:CDS
ATAAGCTTACCCTTTGCACT+AGG 0.536559 5.2:-3463413 MS.gene21627:CDS
ATAGATAATTGAGTCATCAC+TGG 0.537461 5.2:+3461635 None:intergenic
CGCATTTCTTGTATATATGG+GGG 0.537612 5.2:-3463336 MS.gene21627:CDS
TCTGGCTGCTGGGTTTGTCA+CGG 0.539409 5.2:-3462994 MS.gene21627:CDS
ACCAACATAGCTACCATTCA+GGG 0.542167 5.2:-3462819 MS.gene21627:CDS
CAGATCACCAAAGTTGATGT+TGG 0.543622 5.2:-3461732 MS.gene21627:CDS
ATGGAATATTCCTTGAACCA+TGG 0.546940 5.2:+3462363 None:intergenic
TGATGCAACAGTATCACAAT+GGG 0.550837 5.2:-3461416 MS.gene21627:CDS
TGGTGACCTTTATCCCAAAA+CGG 0.553372 5.2:+3462203 None:intergenic
TGCTCTTGTGGAGAAAATAG+AGG 0.555546 5.2:-3462895 MS.gene21627:CDS
GAGCATGCACCATGCTCAGT+TGG 0.559655 5.2:-3461945 MS.gene21627:CDS
CAAGCGCTGACGGCCCAAGT+AGG 0.564029 5.2:+3463606 None:intergenic
CAGTTAATGATGTAGCAGCC+TGG 0.564394 5.2:-3463214 MS.gene21627:CDS
ATGGAGGAACATTCACCTGA+TGG 0.565713 5.2:-3461369 MS.gene21627:CDS
TGAAAATTGGAAGGACAATG+AGG 0.565770 5.2:+3463090 None:intergenic
AACCACTAGTGTCGGCAGAA+TGG 0.566373 5.2:-3463252 MS.gene21627:CDS
CATATCTGTAAAATAGCCAA+TGG 0.568781 5.2:+3464226 None:intergenic
CACAAGAGGAGTGGTCATGA+AGG 0.569719 5.2:+3462484 None:intergenic
CCATCCATTTGAAAATTGGA+AGG 0.571739 5.2:+3463081 None:intergenic
CCTCGAACGCCACAATAACA+TGG 0.571844 5.2:+3462168 None:intergenic
AGCACTAAAGACTCGTTCAA+AGG 0.572987 5.2:+3462702 None:intergenic
GCGGAATTAGGACCTGTTGG+TGG 0.575797 5.2:-3461483 MS.gene21627:CDS
CTTGTTCTTGCAGTCACAAG+GGG 0.579341 5.2:-3464202 MS.gene21627:CDS
TGCGCGGAATTAGGACCTGT+TGG 0.580143 5.2:-3461486 MS.gene21627:CDS
GCTCGTATGGCCGAACACCC+TGG 0.581362 5.2:-3461804 MS.gene21627:CDS
GGTTCATCGTTTGTTCTACA+GGG 0.584720 5.2:-3463386 MS.gene21627:CDS
ATTCAGGGGCTGCAGTCATG+GGG 0.586038 5.2:-3462804 MS.gene21627:CDS
CGGGCAAACATTTCGAGCTG+TGG 0.586544 5.2:+3464312 None:intergenic
ACAATGCCATGAAGGTGAGC+CGG 0.586955 5.2:-3463054 MS.gene21627:CDS
AGCGTTTGCCGGTGCTCTTG+TGG 0.587306 5.2:-3462907 MS.gene21627:CDS
CATGAAAACCATAATAGCAA+AGG 0.589901 5.2:+3462520 None:intergenic
GAATTAATTGTCCTCAACAT+CGG 0.591363 5.2:-3462648 MS.gene21627:CDS
GAGGTCCTCATCGATACTAA+TGG 0.592793 5.2:-3462252 MS.gene21627:CDS
TCTTGTTCTTGCAGTCACAA+GGG 0.592967 5.2:-3464203 MS.gene21627:CDS
TTAGACGCAGGCTGCATCGG+CGG 0.594119 5.2:+3464289 None:intergenic
GTGATGCAACAGTATCACAA+TGG 0.594247 5.2:-3461417 MS.gene21627:CDS
AACAGGCAAGAATCCTCAGT+TGG 0.594260 5.2:+3462387 None:intergenic
TTAGATACTCTAGCAAACCT+TGG 0.598078 5.2:-3463530 MS.gene21627:CDS
TGAACCAATTCCTAGTGCAA+AGG 0.603458 5.2:+3463403 None:intergenic
ACAAATACAGAACAATTGAA+AGG 0.605182 5.2:-3462424 MS.gene21627:CDS
TTATATGCAGCTAACACAAG+AGG 0.606611 5.2:+3462470 None:intergenic
TTCCGGCAATAGCAATGGCA+AGG 0.606740 5.2:+3463435 None:intergenic
GCTTATTCCGGCAATAGCAA+TGG 0.606834 5.2:+3463430 None:intergenic
AAACTTTGTCCCAGAAATGG+AGG 0.612453 5.2:-3461385 MS.gene21627:CDS
AACTTGATTGAGGCTTCAAG+AGG 0.612831 5.2:-3462332 MS.gene21627:CDS
GACAGGGAGGAATCCTACTT+GGG 0.614800 5.2:-3463619 MS.gene21627:intron
AATACTGGAAGACTCTTGGG+TGG 0.618474 5.2:+3463551 None:intergenic
AGATTCTGTCCATGTTATTG+TGG 0.620026 5.2:-3462177 MS.gene21627:CDS
AAACTTCTAACCACTAGTGT+CGG 0.620589 5.2:-3463260 MS.gene21627:CDS
ATGGCCCAACAATGCCATGA+AGG 0.621341 5.2:-3463062 MS.gene21627:CDS
CTTGTGGGTCGAAGGCCGGA+CGG 0.622464 5.2:-3461540 MS.gene21627:CDS
TAACATGGACAGAATCTCCA+TGG 0.622510 5.2:+3462183 None:intergenic
GCAGAAATTAATCACGCGGA+TGG 0.623209 5.2:+3461572 None:intergenic
CACTTGACACAAAATAAAGT+GGG 0.625867 5.2:+3462850 None:intergenic
TTGTGCCATTAGTATCGATG+AGG 0.638116 5.2:+3462247 None:intergenic
CCAAAACAGATGCTGTTGTG+CGG 0.640724 5.2:+3461439 None:intergenic
AAAATGCAGACGGCCAACTG+AGG 0.641897 5.2:-3462400 MS.gene21627:CDS
AATGGAAGTGCATAATCAAG+AGG 0.642363 5.2:+3464244 None:intergenic
AGCCATTCTGCCGACACTAG+TGG 0.642910 5.2:+3463250 None:intergenic
AAATCTCTGCGGAAAACTGG+AGG 0.646572 5.2:-3463464 MS.gene21627:CDS
ATACTGGAAGACTCTTGGGT+GGG 0.647325 5.2:+3463552 None:intergenic
AAACCACTAATCTTATGCCA+GGG 0.648115 5.2:+3461787 None:intergenic
TCTTGAAAGAAGCGAGTGCG+CGG 0.650120 5.2:-3461502 MS.gene21627:CDS
ACATTCACCTGATGGAGACA+CGG 0.653891 5.2:-3461361 MS.gene21627:CDS
TGCAGCTAACACAAGAGGAG+TGG 0.658853 5.2:+3462475 None:intergenic
ACAATCTCTGCAATGACTCG+AGG 0.658940 5.2:+3464148 None:intergenic
CTACAGGGAACAATTGCCAA+AGG 0.662191 5.2:-3463371 MS.gene21627:CDS
AGTTGCAGAAATTAATCACG+CGG 0.663635 5.2:+3461568 None:intergenic
ATGGAAGTGCATAATCAAGA+GGG 0.669234 5.2:+3464245 None:intergenic
ATAGAAAGAATATAAGACCA+AGG 0.670462 5.2:+3463513 None:intergenic
TGAAATACACCATTAGACGC+AGG 0.678423 5.2:+3464277 None:intergenic
CCCCATATATACAAGAAATG+CGG 0.682370 5.2:+3463337 None:intergenic
TCATTGGTCGAACACACACG+TGG 0.711445 5.2:+3462129 None:intergenic
AGTTAGATCCTAAATCTCTG+CGG 0.716660 5.2:-3463475 MS.gene21627:CDS
CCAACATAGCTACCATTCAG+GGG 0.726005 5.2:-3462818 MS.gene21627:CDS
AAATGGAAGAATGATGACAG+CGG 0.728068 5.2:+3462043 None:intergenic
GATGCAACAGTATCACAATG+GGG 0.828892 5.2:-3461415 MS.gene21627:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCCTATAATTAATTTAAAA+TGG - chr5.2:3463094-3463113 MS.gene21627:CDS 10.0%
!! AATTAATTATAGGAATAATG+AGG + chr5.2:3463089-3463108 None:intergenic 15.0%
!! ACTGTGAAATTATATAAAAA+TGG + chr5.2:3461706-3461725 None:intergenic 15.0%
!! TTTATATATTAGAAACTAGT+GGG - chr5.2:3461640-3461659 MS.gene21627:CDS 15.0%
!!! CACCATTTTAAATTAATTAT+AGG + chr5.2:3463099-3463118 None:intergenic 15.0%
!!! TTTTATATATTAGAAACTAG+TGG - chr5.2:3461639-3461658 MS.gene21627:CDS 15.0%
!! TTATATATTAGAAACTAGTG+GGG - chr5.2:3461641-3461660 MS.gene21627:CDS 20.0%
!!! ATATTCTTTCTATTTTTAGC+AGG - chr5.2:3462172-3462191 MS.gene21627:CDS 20.0%
! AACTTCATTAAATCCACATT+TGG - chr5.2:3461947-3461966 MS.gene21627:CDS 25.0%
! AATGATAGCAATTTCATCAA+TGG - chr5.2:3463564-3463583 MS.gene21627:CDS 25.0%
! ACAAATACAGAACAATTGAA+AGG - chr5.2:3463251-3463270 MS.gene21627:CDS 25.0%
! ATAGAAAGAATATAAGACCA+AGG + chr5.2:3462165-3462184 None:intergenic 25.0%
! ATATAAAAATGGAACACTCA+AGG + chr5.2:3461695-3461714 None:intergenic 25.0%
! ATTGATGAAATTGCTATCAT+TGG + chr5.2:3463565-3463584 None:intergenic 25.0%
! TTATTGGAACCTAATATTGT+AGG - chr5.2:3463919-3463938 MS.gene21627:intron 25.0%
!! GGATTTTTGATGAATAGTAA+AGG - chr5.2:3462997-3463016 MS.gene21627:CDS 25.0%
!! TATAAGAAACGATTTTCGAT+TGG - chr5.2:3463690-3463709 MS.gene21627:intron 25.0%
!! TTAGAACTTGTTTCATAGTT+TGG + chr5.2:3461756-3461775 None:intergenic 25.0%
!!! ATTTTCGATTGGTTAACAAA+AGG - chr5.2:3463701-3463720 MS.gene21627:intron 25.0%
!!! TCTAGTTTTCGTTACATTTA+CGG - chr5.2:3462897-3462916 MS.gene21627:CDS 25.0%
AAAATAGAGGATCTTGTATC+TGG - chr5.2:3462793-3462812 MS.gene21627:CDS 30.0%
AATAAACTTTGTCCCAGAAA+TGG - chr5.2:3464287-3464306 MS.gene21627:CDS 30.0%
ACCTCTCACAAAATTCTTTA+AGG + chr5.2:3463408-3463427 None:intergenic 30.0%
ATAGATAATTGAGTCATCAC+TGG + chr5.2:3464043-3464062 None:intergenic 30.0%
ATCGATGATAAACTTGATTG+AGG - chr5.2:3463333-3463352 MS.gene21627:CDS 30.0%
ATTGAAAGGAAAAATGCAGA+CGG - chr5.2:3463265-3463284 MS.gene21627:CDS 30.0%
CACAAAAGGTAAGACAAAAT+GGG - chr5.2:3463446-3463465 MS.gene21627:CDS 30.0%
CACTTGACACAAAATAAAGT+GGG + chr5.2:3462828-3462847 None:intergenic 30.0%
CATATCTGTAAAATAGCCAA+TGG + chr5.2:3461452-3461471 None:intergenic 30.0%
CATATGTATAACTCATCAAC+CGG + chr5.2:3462643-3462662 None:intergenic 30.0%
CATGAAAACCATAATAGCAA+AGG + chr5.2:3463158-3463177 None:intergenic 30.0%
GAATTAATTGTCCTCAACAT+CGG - chr5.2:3463027-3463046 MS.gene21627:CDS 30.0%
TCAATCAAGTTTATCATCGA+TGG + chr5.2:3463334-3463353 None:intergenic 30.0%
TCCGCATTTCTTGTATATAT+GGG - chr5.2:3462337-3462356 MS.gene21627:CDS 30.0%
TTCCATTTCATAAGCAACAA+AGG - chr5.2:3463647-3463666 MS.gene21627:intron 30.0%
TTCCGCATTTCTTGTATATA+TGG - chr5.2:3462336-3462355 MS.gene21627:CDS 30.0%
TTCTGCAACTTAATTCTTGT+GGG - chr5.2:3464120-3464139 MS.gene21627:intron 30.0%
TTTCTGCAACTTAATTCTTG+TGG - chr5.2:3464119-3464138 MS.gene21627:intron 30.0%
TTTGATGAATAGTAAAGGCT+TGG - chr5.2:3463002-3463021 MS.gene21627:CDS 30.0%
! AACCTTTGTTGCTTATGAAA+TGG + chr5.2:3463652-3463671 None:intergenic 30.0%
! ACCTTAAAGAATTTTGTGAG+AGG - chr5.2:3463404-3463423 MS.gene21627:CDS 30.0%
! TCTTAAACATGTGCACATTT+TGG + chr5.2:3461876-3461895 None:intergenic 30.0%
! TTGTCCTTCCAATTTTCAAA+TGG - chr5.2:3462590-3462609 MS.gene21627:intron 30.0%
! TTTGCTAGAGTATCTAATAC+TGG + chr5.2:3462142-3462161 None:intergenic 30.0%
!! ATTGCTTATTGATGATGACA+GGG - chr5.2:3462040-3462059 MS.gene21627:CDS 30.0%
!! ATTTCTGGGACAAAGTTTAT+TGG + chr5.2:3464288-3464307 None:intergenic 30.0%
!! CCTATCTTCTAAAACCTTTA+AGG - chr5.2:3461501-3461520 MS.gene21627:CDS 30.0%
!! TTACATTTACGGCTTGTTTT+GGG - chr5.2:3462908-3462927 MS.gene21627:CDS 30.0%
!!! AGTGGTTAGAAGTTTTAACT+CGG + chr5.2:3462410-3462429 None:intergenic 30.0%
!!! CCTTAAAGGTTTTAGAAGAT+AGG + chr5.2:3461504-3461523 None:intergenic 30.0%
!!! CTTTGCTATTATGGTTTTCA+TGG - chr5.2:3463156-3463175 MS.gene21627:CDS 30.0%
AAAACCACTAATCTTATGCC+AGG + chr5.2:3463892-3463911 None:intergenic 35.0%
AAACCACTAATCTTATGCCA+GGG + chr5.2:3463891-3463910 None:intergenic 35.0%
AAACTTCTAACCACTAGTGT+CGG - chr5.2:3462415-3462434 MS.gene21627:CDS 35.0%
AAATGGAAGAATGATGACAG+CGG + chr5.2:3463635-3463654 None:intergenic 35.0%
ACAAAAATTCCAACTGAGCA+TGG + chr5.2:3463742-3463761 None:intergenic 35.0%
ACAATGACACAAATGGAGAT+TGG + chr5.2:3462525-3462544 None:intergenic 35.0%
AGATTCTGTCCATGTTATTG+TGG - chr5.2:3463498-3463517 MS.gene21627:CDS 35.0%
AGTTAGATCCTAAATCTCTG+CGG - chr5.2:3462200-3462219 MS.gene21627:CDS 35.0%
AGTTGCAGAAATTAATCACG+CGG + chr5.2:3464110-3464129 None:intergenic 35.0%
ATCGATACTAATGGCACAAA+AGG - chr5.2:3463432-3463451 MS.gene21627:CDS 35.0%
ATCTAATACTGGAAGACTCT+TGG + chr5.2:3462131-3462150 None:intergenic 35.0%
ATCTGATACTGAATTACTGC+TGG + chr5.2:3463977-3463996 None:intergenic 35.0%
ATGGAATATTCCTTGAACCA+TGG + chr5.2:3463315-3463334 None:intergenic 35.0%
CAACATCGGTAAAGATAGAA+AGG - chr5.2:3463041-3463060 MS.gene21627:CDS 35.0%
CATAAGCAACAAAGGTTAGA+CGG - chr5.2:3463655-3463674 MS.gene21627:intron 35.0%
CCACTTGACACAAAATAAAG+TGG + chr5.2:3462829-3462848 None:intergenic 35.0%
CCATCCATTTGAAAATTGGA+AGG + chr5.2:3462597-3462616 None:intergenic 35.0%
CCCCATATATACAAGAAATG+CGG + chr5.2:3462341-3462360 None:intergenic 35.0%
CCGCATTTCTTGTATATATG+GGG - chr5.2:3462338-3462357 MS.gene21627:CDS 35.0%
CGCATTTCTTGTATATATGG+GGG - chr5.2:3462339-3462358 MS.gene21627:CDS 35.0%
GCACAAAAGGTAAGACAAAA+TGG - chr5.2:3463445-3463464 MS.gene21627:CDS 35.0%
GTGATCTGTCCTACAATATT+AGG + chr5.2:3463931-3463950 None:intergenic 35.0%
TCTAATACTGGAAGACTCTT+GGG + chr5.2:3462130-3462149 None:intergenic 35.0%
TCTTGATTATGCACTTCCAT+TGG - chr5.2:3461433-3461452 MS.gene21627:CDS 35.0%
TGAAAATTGGAAGGACAATG+AGG + chr5.2:3462588-3462607 None:intergenic 35.0%
TGATGCAACAGTATCACAAT+GGG - chr5.2:3464259-3464278 MS.gene21627:CDS 35.0%
TTAGAAACTAGTGGGGAAAT+TGG - chr5.2:3461648-3461667 MS.gene21627:CDS 35.0%
TTAGATACTCTAGCAAACCT+TGG - chr5.2:3462145-3462164 MS.gene21627:CDS 35.0%
TTAGTAAGACTGACCAAATG+TGG + chr5.2:3461963-3461982 None:intergenic 35.0%
TTATATGCAGCTAACACAAG+AGG + chr5.2:3463208-3463227 None:intergenic 35.0%
TTCTATCTTTACCGATGTTG+AGG + chr5.2:3463041-3463060 None:intergenic 35.0%
TTGTGTTAGCTGCATATAAG+AGG - chr5.2:3463209-3463228 MS.gene21627:CDS 35.0%
! AATCTCCATTTGTGTCATTG+TGG - chr5.2:3462524-3462543 MS.gene21627:CDS 35.0%
! AATGGAAGTGCATAATCAAG+AGG + chr5.2:3461434-3461453 None:intergenic 35.0%
! AGTGGTTTTTCGCTTCTTAT+TGG - chr5.2:3463903-3463922 MS.gene21627:intron 35.0%
! ATCTCCATTTGTGTCATTGT+GGG - chr5.2:3462525-3462544 MS.gene21627:CDS 35.0%
! ATGGAAGTGCATAATCAAGA+GGG + chr5.2:3461433-3461452 None:intergenic 35.0%
! ATTGTTGTGCTTTTCTTTGG+TGG - chr5.2:3463817-3463836 MS.gene21627:intron 35.0%
! CCTTCCAATTTTCAAATGGA+TGG - chr5.2:3462594-3462613 MS.gene21627:intron 35.0%
! GAAAAGCACAACAATGCAAT+AGG + chr5.2:3463812-3463831 None:intergenic 35.0%
! GTTGGAATTTTTGTTGATCG+CGG - chr5.2:3463748-3463767 MS.gene21627:intron 35.0%
! TGCATTGTTGTGCTTTTCTT+TGG - chr5.2:3463814-3463833 MS.gene21627:intron 35.0%
! TTGGAATTTTTGTTGATCGC+GGG - chr5.2:3463749-3463768 MS.gene21627:intron 35.0%
!! AACCTCTGCTCTATTTTAGA+AGG + chr5.2:3461989-3462008 None:intergenic 35.0%
!! GATTGCTTATTGATGATGAC+AGG - chr5.2:3462039-3462058 MS.gene21627:CDS 35.0%
!! GTTACATTTACGGCTTGTTT+TGG - chr5.2:3462907-3462926 MS.gene21627:CDS 35.0%
!! TGATCAGACCTTTGCTATTA+TGG - chr5.2:3463147-3463166 MS.gene21627:CDS 35.0%
!! TTTTAACTCGGCCAAAATAC+GGG + chr5.2:3462398-3462417 None:intergenic 35.0%
!!! CCACTTTATTTTGTGTCAAG+TGG - chr5.2:3462826-3462845 MS.gene21627:CDS 35.0%
!!! CGCGTTTGTTTTTGGAATTT+TGG - chr5.2:3462732-3462751 MS.gene21627:CDS 35.0%
!!! CTTGTTTTGGGAAGATTGTT+GGG - chr5.2:3462920-3462939 MS.gene21627:CDS 35.0%
!!! GGAATTTTGGTTCCAAAAGA+TGG - chr5.2:3462745-3462764 MS.gene21627:CDS 35.0%
!!! GTTTTAACTCGGCCAAAATA+CGG + chr5.2:3462399-3462418 None:intergenic 35.0%
AAAAACAAACGCGCCAAACA+TGG + chr5.2:3462725-3462744 None:intergenic 40.0%
AAACCTGCAGATACGATGAT+CGG + chr5.2:3463123-3463142 None:intergenic 40.0%
AAACTTTGTCCCAGAAATGG+AGG - chr5.2:3464290-3464309 MS.gene21627:CDS 40.0%
AACATTTCGAGCTGTGGTAT+TGG + chr5.2:3461360-3461379 None:intergenic 40.0%
AACTTGATTGAGGCTTCAAG+AGG - chr5.2:3463343-3463362 MS.gene21627:CDS 40.0%
AAGACCCACAATGACACAAA+TGG + chr5.2:3462532-3462551 None:intergenic 40.0%
ACCAACATAGCTACCATTCA+GGG - chr5.2:3462856-3462875 MS.gene21627:CDS 40.0%
ACTTAATTCTTGTGGGTCGA+AGG - chr5.2:3464127-3464146 MS.gene21627:intron 40.0%
AGAGTCTCCAACATCAACTT+TGG + chr5.2:3463953-3463972 None:intergenic 40.0%
AGCACTAAAGACTCGTTCAA+AGG + chr5.2:3462976-3462995 None:intergenic 40.0%
ATAAGCTTACCCTTTGCACT+AGG - chr5.2:3462262-3462281 MS.gene21627:CDS 40.0%
ATCGGAATTCATGCCATGTT+TGG - chr5.2:3462709-3462728 MS.gene21627:CDS 40.0%
ATTCCTTGAACCATGGAAAC+AGG + chr5.2:3463308-3463327 None:intergenic 40.0%
CATTGTGGGTCTTCTTAAGT+GGG - chr5.2:3462539-3462558 MS.gene21627:CDS 40.0%
GAACCAATTCCTAGTGCAAA+GGG + chr5.2:3462274-3462293 None:intergenic 40.0%
GATGCAACAGTATCACAATG+GGG - chr5.2:3464260-3464279 MS.gene21627:CDS 40.0%
GCAGAGAGTTTAAACACACT+TGG - chr5.2:3461924-3461943 MS.gene21627:CDS 40.0%
GCGTCTAATGGTGTATTTCA+AGG - chr5.2:3461401-3461420 MS.gene21627:CDS 40.0%
GGTTCATCGTTTGTTCTACA+GGG - chr5.2:3462289-3462308 MS.gene21627:CDS 40.0%
GTGAATGTTCCTCCATTTCT+GGG + chr5.2:3464302-3464321 None:intergenic 40.0%
GTGATGCAACAGTATCACAA+TGG - chr5.2:3464258-3464277 MS.gene21627:CDS 40.0%
TAACATGGACAGAATCTCCA+TGG + chr5.2:3463495-3463514 None:intergenic 40.0%
TCATTGTGGGTCTTCTTAAG+TGG - chr5.2:3462538-3462557 MS.gene21627:CDS 40.0%
TCTTGTTCTTGCAGTCACAA+GGG - chr5.2:3461472-3461491 MS.gene21627:CDS 40.0%
TGAAATACACCATTAGACGC+AGG + chr5.2:3461401-3461420 None:intergenic 40.0%
TGAACCAATTCCTAGTGCAA+AGG + chr5.2:3462275-3462294 None:intergenic 40.0%
TGCAAAGGGTAAGCTTATTC+CGG + chr5.2:3462260-3462279 None:intergenic 40.0%
TGCTCTTGTGGAGAAAATAG+AGG - chr5.2:3462780-3462799 MS.gene21627:CDS 40.0%
TGGGCCATCCATTTGAAAAT+TGG + chr5.2:3462601-3462620 None:intergenic 40.0%
TGGTGACCTTTATCCCAAAA+CGG + chr5.2:3463475-3463494 None:intergenic 40.0%
TGGTTCATCGTTTGTTCTAC+AGG - chr5.2:3462288-3462307 MS.gene21627:CDS 40.0%
TTACCCTTTGCACTAGGAAT+TGG - chr5.2:3462268-3462287 MS.gene21627:CDS 40.0%
TTGAACGAGTCTTTAGTGCT+TGG - chr5.2:3462976-3462995 MS.gene21627:CDS 40.0%
! AGATTGGCTATTGCCTGATA+AGG + chr5.2:3462509-3462528 None:intergenic 40.0%
! TAATCTTATGCCAGGGTGTT+CGG + chr5.2:3463884-3463903 None:intergenic 40.0%
! TCCTGTATTAGCCCGTATTT+TGG - chr5.2:3462384-3462403 MS.gene21627:CDS 40.0%
!! ATGTTTGGCGCGTTTGTTTT+TGG - chr5.2:3462724-3462743 MS.gene21627:CDS 40.0%
!! CAGATCACCAAAGTTGATGT+TGG - chr5.2:3463943-3463962 MS.gene21627:intron 40.0%
!! TATTTTCTCCACAAGAGCAC+CGG + chr5.2:3462779-3462798 None:intergenic 40.0%
!! TGCCTTCTAAAATAGAGCAG+AGG - chr5.2:3461984-3462003 MS.gene21627:CDS 40.0%
!! TGCGGAAGTATTTACACCTT+TGG + chr5.2:3462323-3462342 None:intergenic 40.0%
!! TTGTGCCATTAGTATCGATG+AGG + chr5.2:3463431-3463450 None:intergenic 40.0%
!!! ACAAAATGGGTTGCCGTTTT+GGG - chr5.2:3463459-3463478 MS.gene21627:CDS 40.0%
!!! GCTTGTTTTGGGAAGATTGT+TGG - chr5.2:3462919-3462938 MS.gene21627:CDS 40.0%
!!! TTTTGGGATAAAGGTCACCA+TGG - chr5.2:3463475-3463494 MS.gene21627:CDS 40.0%
AAATCTCTGCGGAAAACTGG+AGG - chr5.2:3462211-3462230 MS.gene21627:CDS 45.0%
AACAGGCAAGAATCCTCAGT+TGG + chr5.2:3463291-3463310 None:intergenic 45.0%
AATACTGGAAGACTCTTGGG+TGG + chr5.2:3462127-3462146 None:intergenic 45.0%
ACAATCTCTGCAATGACTCG+AGG + chr5.2:3461530-3461549 None:intergenic 45.0%
ACACCCTGGCATAAGATTAG+TGG - chr5.2:3463885-3463904 MS.gene21627:intron 45.0%
ACATTCACCTGATGGAGACA+CGG - chr5.2:3464314-3464333 MS.gene21627:CDS 45.0%
AGCAAGAGCAAGAAGAATCC+AGG + chr5.2:3462482-3462501 None:intergenic 45.0%
AGGATTCTTGCCTGTTTCCA+TGG - chr5.2:3463295-3463314 MS.gene21627:CDS 45.0%
ATACTGGAAGACTCTTGGGT+GGG + chr5.2:3462126-3462145 None:intergenic 45.0%
ATGACTCGAGGTTGCCTTAA+AGG + chr5.2:3461518-3461537 None:intergenic 45.0%
ATGGAGGAACATTCACCTGA+TGG - chr5.2:3464306-3464325 MS.gene21627:CDS 45.0%
CAGTTAATGATGTAGCAGCC+TGG - chr5.2:3462461-3462480 MS.gene21627:CDS 45.0%
CCAAAACAGATGCTGTTGTG+CGG + chr5.2:3464239-3464258 None:intergenic 45.0%
CCAACATAGCTACCATTCAG+GGG - chr5.2:3462857-3462876 MS.gene21627:CDS 45.0%
CCTAAATCTCTGCGGAAAAC+TGG - chr5.2:3462208-3462227 MS.gene21627:CDS 45.0%
CTACAGGGAACAATTGCCAA+AGG - chr5.2:3462304-3462323 MS.gene21627:CDS 45.0%
CTCACCTTCATGGCATTGTT+GGG + chr5.2:3462620-3462639 None:intergenic 45.0%
CTCCATAAGCAAGAGCTTCA+CGG + chr5.2:3463852-3463871 None:intergenic 45.0%
CTCGCTTCTTTCAAGAGCAA+AGG + chr5.2:3464169-3464188 None:intergenic 45.0%
CTTGTTCTTGCAGTCACAAG+GGG - chr5.2:3461473-3461492 MS.gene21627:CDS 45.0%
GACCAACATAGCTACCATTC+AGG - chr5.2:3462855-3462874 MS.gene21627:CDS 45.0%
GAGGTCCTCATCGATACTAA+TGG - chr5.2:3463423-3463442 MS.gene21627:CDS 45.0%
GCAGAAATTAATCACGCGGA+TGG + chr5.2:3464106-3464125 None:intergenic 45.0%
GCTTATTCCGGCAATAGCAA+TGG + chr5.2:3462248-3462267 None:intergenic 45.0%
GGTGAATGTTCCTCCATTTC+TGG + chr5.2:3464303-3464322 None:intergenic 45.0%
TCTTGCTTATGGAGCTCGTA+TGG - chr5.2:3463858-3463877 MS.gene21627:intron 45.0%
TGCTACATTAGCTGCTGTTC+TGG - chr5.2:3462663-3462682 MS.gene21627:CDS 45.0%
TTGCCTGTTTCCATGGTTCA+AGG - chr5.2:3463302-3463321 MS.gene21627:CDS 45.0%
! ATTTTTGTTGATCGCGGGCT+TGG - chr5.2:3463754-3463773 MS.gene21627:intron 45.0%
! CCAGTTTTCCGCAGAGATTT+AGG + chr5.2:3462211-3462230 None:intergenic 45.0%
! GCCAAAATACGGGCTAATAC+AGG + chr5.2:3462388-3462407 None:intergenic 45.0%
! GTCTTGTTCTTGCAGTCACA+AGG - chr5.2:3461471-3461490 MS.gene21627:CDS 45.0%
! GTTGTGCTTTTCTTTGGTGG+TGG - chr5.2:3463820-3463839 MS.gene21627:intron 45.0%
!! CACACACGTGGCTTAGTTTT+TGG + chr5.2:3463537-3463556 None:intergenic 45.0%
!! GCTTATTGATGATGACAGGG+AGG - chr5.2:3462043-3462062 MS.gene21627:CDS 45.0%
!!! GACAAAATGGGTTGCCGTTT+TGG - chr5.2:3463458-3463477 MS.gene21627:CDS 45.0%
AAAATGCAGACGGCCAACTG+AGG - chr5.2:3463275-3463294 MS.gene21627:CDS 50.0%
AATTCTTGTGGGTCGAAGGC+CGG - chr5.2:3464131-3464150 MS.gene21627:intron 50.0%
ACAATGCCATGAAGGTGAGC+CGG - chr5.2:3462621-3462640 MS.gene21627:intron 50.0%
ATGGCCCAACAATGCCATGA+AGG - chr5.2:3462613-3462632 MS.gene21627:intron 50.0%
CACAAGAGGAGTGGTCATGA+AGG + chr5.2:3463194-3463213 None:intergenic 50.0%
CCATGTTATTGTGGCGTTCG+AGG - chr5.2:3463507-3463526 MS.gene21627:CDS 50.0%
CCCCTGAATGGTAGCTATGT+TGG + chr5.2:3462860-3462879 None:intergenic 50.0%
CCTCGAACGCCACAATAACA+TGG + chr5.2:3463510-3463529 None:intergenic 50.0%
GAAGATTCCGTGTCTCCATC+AGG + chr5.2:3464324-3464343 None:intergenic 50.0%
GACAGGGAGGAATCCTACTT+GGG - chr5.2:3462056-3462075 MS.gene21627:CDS 50.0%
GACCGTGAAGCTCTTGCTTA+TGG - chr5.2:3463847-3463866 MS.gene21627:intron 50.0%
GCTCACCTTCATGGCATTGT+TGG + chr5.2:3462621-3462640 None:intergenic 50.0%
GGGTTTGTCACGGATGCTAT+CGG - chr5.2:3462691-3462710 MS.gene21627:CDS 50.0%
TCATTGGTCGAACACACACG+TGG + chr5.2:3463549-3463568 None:intergenic 50.0%
TCTTGAAAGAAGCGAGTGCG+CGG - chr5.2:3464173-3464192 MS.gene21627:CDS 50.0%
TGACAGGGAGGAATCCTACT+TGG - chr5.2:3462055-3462074 MS.gene21627:CDS 50.0%
TGACCGATCATCGTATCTGC+AGG - chr5.2:3463117-3463136 MS.gene21627:CDS 50.0%
TGCAGCTAACACAAGAGGAG+TGG + chr5.2:3463203-3463222 None:intergenic 50.0%
TGGGTCTTCTTAAGTGGGTG+TGG - chr5.2:3462544-3462563 MS.gene21627:intron 50.0%
TTCCGGCAATAGCAATGGCA+AGG + chr5.2:3462243-3462262 None:intergenic 50.0%
! CCAAAAGATGGAGCGTTTGC+CGG - chr5.2:3462757-3462776 MS.gene21627:CDS 50.0%
! CCGCACAACAGCATCTGTTT+TGG - chr5.2:3464236-3464255 MS.gene21627:CDS 50.0%
! GTCCTTGCCATTGCTATTGC+CGG - chr5.2:3462238-3462257 MS.gene21627:CDS 50.0%
!! AACCACTAGTGTCGGCAGAA+TGG - chr5.2:3462423-3462442 MS.gene21627:CDS 50.0%
!! GCTCTTGCTGTTGCCTTATC+AGG - chr5.2:3462493-3462512 MS.gene21627:CDS 50.0%
!! TTATTCCGTCCAAGCGCTGA+CGG + chr5.2:3462082-3462101 None:intergenic 50.0%
!!! GGGTTGCCGTTTTGGGATAA+AGG - chr5.2:3463466-3463485 MS.gene21627:CDS 50.0%
AGAAGCGAGTGCGCGGAATT+AGG - chr5.2:3464180-3464199 MS.gene21627:CDS 55.0%
AGCCATTCTGCCGACACTAG+TGG + chr5.2:3462428-3462447 None:intergenic 55.0%
ATTCAGGGGCTGCAGTCATG+GGG - chr5.2:3462871-3462890 MS.gene21627:CDS 55.0%
CATTCAGGGGCTGCAGTCAT+GGG - chr5.2:3462870-3462889 MS.gene21627:CDS 55.0%
CCATTAGACGCAGGCTGCAT+CGG + chr5.2:3461392-3461411 None:intergenic 55.0%
CGGGCAAACATTTCGAGCTG+TGG + chr5.2:3461366-3461385 None:intergenic 55.0%
GAGCATGCACCATGCTCAGT+TGG - chr5.2:3463730-3463749 MS.gene21627:intron 55.0%
GATGCCAACAAGCCACCAAC+AGG + chr5.2:3464207-3464226 None:intergenic 55.0%
GCAAAGGCCAATTCACCGTC+CGG + chr5.2:3464153-3464172 None:intergenic 55.0%
GCGGAATTAGGACCTGTTGG+TGG - chr5.2:3464192-3464211 MS.gene21627:CDS 55.0%
TCTGGCTGCTGGGTTTGTCA+CGG - chr5.2:3462681-3462700 MS.gene21627:CDS 55.0%
TCTTGCAGTCACAAGGGGAC+TGG - chr5.2:3461478-3461497 MS.gene21627:CDS 55.0%
TGCGCGGAATTAGGACCTGT+TGG - chr5.2:3464189-3464208 MS.gene21627:CDS 55.0%
TTTGTTGATCGCGGGCTTGG+TGG - chr5.2:3463757-3463776 MS.gene21627:intron 55.0%
! CATCAACCGGCTCACCTTCA+TGG + chr5.2:3462630-3462649 None:intergenic 55.0%
! CCGGCAAACGCTCCATCTTT+TGG + chr5.2:3462760-3462779 None:intergenic 55.0%
! TAGCTGCTGTTCTGGCTGCT+GGG - chr5.2:3462671-3462690 MS.gene21627:CDS 55.0%
! TTAGCTGCTGTTCTGGCTGC+TGG - chr5.2:3462670-3462689 MS.gene21627:CDS 55.0%
!! AGGACCTGTTGGTGGCTTGT+TGG - chr5.2:3464200-3464219 MS.gene21627:CDS 55.0%
AGCGTTTGCCGGTGCTCTTG+TGG - chr5.2:3462768-3462787 MS.gene21627:CDS 60.0%
CCATGACTGCAGCCCCTGAA+TGG + chr5.2:3462872-3462891 None:intergenic 60.0%
CCATTCAGGGGCTGCAGTCA+TGG - chr5.2:3462869-3462888 MS.gene21627:CDS 60.0%
CCGATGCAGCCTGCGTCTAA+TGG - chr5.2:3461389-3461408 MS.gene21627:CDS 60.0%
TCGAAGGCCGGACGGTGAAT+TGG - chr5.2:3464143-3464162 MS.gene21627:intron 60.0%
TTAGACGCAGGCTGCATCGG+CGG + chr5.2:3461389-3461408 None:intergenic 60.0%
CTACTTGGGCCGTCAGCGCT+TGG - chr5.2:3462070-3462089 MS.gene21627:CDS 65.0%
CTTGTGGGTCGAAGGCCGGA+CGG - chr5.2:3464135-3464154 MS.gene21627:intron 65.0%
GCTCGTATGGCCGAACACCC+TGG - chr5.2:3463871-3463890 MS.gene21627:intron 65.0%
TTGGGCCGTCAGCGCTTGGA+CGG - chr5.2:3462074-3462093 MS.gene21627:CDS 65.0%
!! CAAGCGCTGACGGCCCAAGT+AGG + chr5.2:3462072-3462091 None:intergenic 65.0%
! ACGCAGGCTGCATCGGCGGC+GGG + chr5.2:3461385-3461404 None:intergenic 75.0%
! GACGCAGGCTGCATCGGCGG+CGG + chr5.2:3461386-3461405 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 3461350 3464347 3461350 ID=MS.gene21627
chr5.2 mRNA 3461350 3464347 3461350 ID=MS.gene21627.t1;Parent=MS.gene21627
chr5.2 exon 3464147 3464347 3464147 ID=MS.gene21627.t1.exon1;Parent=MS.gene21627.t1
chr5.2 CDS 3464147 3464347 3464147 ID=cds.MS.gene21627.t1;Parent=MS.gene21627.t1
chr5.2 exon 3462635 3463636 3462635 ID=MS.gene21627.t1.exon2;Parent=MS.gene21627.t1
chr5.2 CDS 3462635 3463636 3462635 ID=cds.MS.gene21627.t1;Parent=MS.gene21627.t1
chr5.2 exon 3461350 3462558 3461350 ID=MS.gene21627.t1.exon3;Parent=MS.gene21627.t1
chr5.2 CDS 3461350 3462558 3461350 ID=cds.MS.gene21627.t1;Parent=MS.gene21627.t1
Gene Sequence

>MS.gene21627

ATGACTTCCAATACCACAGCTCGAAATGTTTGCCCGCCGCCGATGCAGCCTGCGTCTAATGGTGTATTTCAAGGAGATCACCCTCTTGATTATGCACTTCCATTGGCTATTTTACAGATATGTCTTGTTCTTGCAGTCACAAGGGGACTGGCCTATCTTCTAAAACCTTTAAGGCAACCTCGAGTCATTGCAGAGATTGTTGTAAGTTCTTTTATTTTTGTTTAAGTATTTACTTAATTTAATGATTTTTTTTTGTTAACTGATATTAATAATTGATATTAGTAAAGAATTTTATATATTAGAAACTAGTGGGGAAATTGGCAAACAATGCAACAATAAGAGCCTTGAGTGTTCCATTTTTATATAATTTCACAGTTTTGAGTTTTAACTACGTTCTACTTGTCCAAACTATGAAACAAGTTCTAATATTTAGTCAATTTTTTATGTAGAGATTAGAATGCAATATGACTATAAAAAATTCACTGTTCTTAAATCAACAATAGATGTTAGAGAAATAGACATGCCAAAATGTGCACATGTTTAAGAGAAATTAAAATTGAATCATATTAAAATCGCAGAGAGTTTAAACACACTTGGAACTTCATTAAATCCACATTTGGTCAGTCTTACTAATTGCCTTCTAAAATAGAGCAGAGGTTTTTTCAACTTCTATTTTTGTGTGTTAATTAGATTGCTTATTGATGATGACAGGGAGGAATCCTACTTGGGCCGTCAGCGCTTGGACGGAATAAAAGCTATCTACATGCAGTTTTCCCACCCAAGAGTCTTCCAGTATTAGATACTCTAGCAAACCTTGGTCTTATATTCTTTCTATTTTTAGCAGGCATAGAGTTAGATCCTAAATCTCTGCGGAAAACTGGAGGTAGAGTCCTTGCCATTGCTATTGCCGGAATAAGCTTACCCTTTGCACTAGGAATTGGTTCATCGTTTGTTCTACAGGGAACAATTGCCAAAGGTGTAAATACTTCCGCATTTCTTGTATATATGGGGGTCGCTCTGTCTATAACTGCTTTTCCTGTATTAGCCCGTATTTTGGCCGAGTTAAAACTTCTAACCACTAGTGTCGGCAGAATGGCTATGTCAGCTGCAGCAGTTAATGATGTAGCAGCCTGGATTCTTCTTGCTCTTGCTGTTGCCTTATCAGGCAATAGCCAATCTCCATTTGTGTCATTGTGGGTCTTCTTAAGTGGGTGTGGTTTTGTTGTATGTTCAATCCTCATTGTCCTTCCAATTTTCAAATGGATGGCCCAACAATGCCATGAAGGTGAGCCGGTTGATGAGTTATACATATGTGCTACATTAGCTGCTGTTCTGGCTGCTGGGTTTGTCACGGATGCTATCGGAATTCATGCCATGTTTGGCGCGTTTGTTTTTGGAATTTTGGTTCCAAAAGATGGAGCGTTTGCCGGTGCTCTTGTGGAGAAAATAGAGGATCTTGTATCTGGTCTATTACTCCCACTTTATTTTGTGTCAAGTGGATTGAAGACCAACATAGCTACCATTCAGGGGCTGCAGTCATGGGGTCTTCTAGTTTTCGTTACATTTACGGCTTGTTTTGGGAAGATTGTTGGGACTATTGTAGTGTCTCTTCTATGTAAAGTACCTTTGAACGAGTCTTTAGTGCTTGGATTTTTGATGAATAGTAAAGGCTTGGTTGAATTAATTGTCCTCAACATCGGTAAAGATAGAAAGGTATTCGAATTTATTTTCTTGCTCCTCATTATTCCTATAATTAATTTAAAATGGTGACCGATCATCGTATCTGCAGGTTTTGAATGATCAGACCTTTGCTATTATGGTTTTCATGGCTCTCATTACTACCTTCATGACCACTCCTCTTGTGTTAGCTGCATATAAGAGGAAAGAAAGAAAATCTAATTACAAATACAGAACAATTGAAAGGAAAAATGCAGACGGCCAACTGAGGATTCTTGCCTGTTTCCATGGTTCAAGGAATATTCCATCGATGATAAACTTGATTGAGGCTTCAAGAGGAATCAAGAAGCATGACACACTTTGTGTCTATGCAATGCACCTTAAAGAATTTTGTGAGAGGTCCTCATCGATACTAATGGCACAAAAGGTAAGACAAAATGGGTTGCCGTTTTGGGATAAAGGTCACCATGGAGATTCTGTCCATGTTATTGTGGCGTTCGAGGCTTACCAAAAACTAAGCCACGTGTGTGTTCGACCAATGATAGCAATTTCATCAATGGCGAATATTCATGAAGACATTTGTGCAACTGCTGATAGAAAGAGAGCCGCTGTCATCATTCTTCCATTTCATAAGCAACAAAGGTTAGACGGTTCACTAGACATTATAAGAAACGATTTTCGATTGGTTAACAAAAGGATACTCGAGCATGCACCATGCTCAGTTGGAATTTTTGTTGATCGCGGGCTTGGTGGTTCATGTCATGTGTCTGCAAGTAATGTTTCCTATTGCATTGTTGTGCTTTTCTTTGGTGGTGGTGACGACCGTGAAGCTCTTGCTTATGGAGCTCGTATGGCCGAACACCCTGGCATAAGATTAGTGGTTTTTCGCTTCTTATTGGAACCTAATATTGTAGGACAGATCACCAAAGTTGATGTTGGAGACTCTTCCAGCAGTAATTCAGTATCAGATTCTGAAGATGACGAGTTTCTTGCTGAATTTAAGCTAAAAACAGCCAGTGATGACTCAATTATCTATGAAGAAAAAATAGTAAAAGATGCTGCAGAAACAGTTGCTACCATCCGCGTGATTAATTTCTGCAACTTAATTCTTGTGGGTCGAAGGCCGGACGGTGAATTGGCCTTTGCTCTTGAAAGAAGCGAGTGCGCGGAATTAGGACCTGTTGGTGGCTTGTTGGCATCACAAGATTTCCGCACAACAGCATCTGTTTTGGTGATGCAACAGTATCACAATGGGGTGCCAATAAACTTTGTCCCAGAAATGGAGGAACATTCACCTGATGGAGACACGGAATCTTCTTAA
Protein sequence

>MS.gene21627.t1

MTSNTTARNVCPPPMQPASNGVFQGDHPLDYALPLAILQICLVLAVTRGLAYLLKPLRQPRVIAEIVGGILLGPSALGRNKSYLHAVFPPKSLPVLDTLANLGLIFFLFLAGIELDPKSLRKTGGRVLAIAIAGISLPFALGIGSSFVLQGTIAKGVNTSAFLVYMGVALSITAFPVLARILAELKLLTTSVGRMAMSAAAVNDVAAWILLALAVALSGNSQSPFVSLWVFLSGCGFVVCSILIVLPIFKWMAQQCHEGEPVDELYICATLAAVLAAGFVTDAIGIHAMFGAFVFGILVPKDGAFAGALVEKIEDLVSGLLLPLYFVSSGLKTNIATIQGLQSWGLLVFVTFTACFGKIVGTIVVSLLCKVPLNESLVLGFLMNSKGLVELIVLNIGKDRKVLNDQTFAIMVFMALITTFMTTPLVLAAYKRKERKSNYKYRTIERKNADGQLRILACFHGSRNIPSMINLIEASRGIKKHDTLCVYAMHLKEFCERSSSILMAQKVRQNGLPFWDKGHHGDSVHVIVAFEAYQKLSHVCVRPMIAISSMANIHEDICATADRKRAAVIILPFHKQQRLDGSLDIIRNDFRLVNKRILEHAPCSVGIFVDRGLGGSCHVSASNVSYCIVVLFFGGGDDREALAYGARMAEHPGIRLVVFRFLLEPNIVGQITKVDVGDSSSSNSVSDSEDDEFLAEFKLKTASDDSIIYEEKIVKDAAETVATIRVINFCNLILVGRRPDGELAFALERSECAELGPVGGLLASQDFRTTASVLVMQQYHNGVPINFVPEMEEHSPDGDTESS