AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22334

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22334.t1 MTR_5g009780 95.143 803 39 0 1 803 1 803 0.0 1535
MS.gene22334.t1 MTR_5g009800 91.688 794 45 2 1 794 1 773 0.0 1462
MS.gene22334.t1 MTR_5g009770 88.903 802 85 2 1 800 1 800 0.0 1453
MS.gene22334.t1 MTR_5g009770 87.779 671 78 2 132 800 1 669 0.0 1204
MS.gene22334.t1 MTR_4g078120 60.837 812 296 6 6 800 3 809 0.0 962
MS.gene22334.t1 MTR_4g123583 58.882 805 315 5 6 797 3 804 0.0 930
MS.gene22334.t1 MTR_8g093780 47.475 792 395 12 4 779 5 791 0.0 721
MS.gene22334.t1 MTR_3g096380 46.477 809 415 10 10 803 13 818 0.0 711
MS.gene22334.t1 MTR_2g011520 45.241 767 410 9 18 779 3 764 0.0 677
MS.gene22334.t1 MTR_7g099800 49.938 811 357 16 15 779 8 815 0.0 671
MS.gene22334.t1 MTR_7g099820 47.342 790 382 13 16 780 5 785 0.0 653
MS.gene22334.t1 MTR_7g099750 48.846 823 341 17 15 779 8 808 0.0 649
MS.gene22334.t1 MTR_7g099820 49.067 697 330 9 16 694 5 694 0.0 625
MS.gene22334.t1 MTR_3g031170 34.447 778 474 11 10 778 28 778 4.53e-139 431
MS.gene22334.t1 MTR_2g040100 33.782 817 488 22 22 802 35 834 8.14e-134 418
MS.gene22334.t1 MTR_3g071990 32.312 783 496 13 6 778 7 765 8.83e-128 402
MS.gene22334.t1 MTR_1g043400 32.416 799 485 20 18 782 11 788 2.63e-124 392
MS.gene22334.t1 MTR_1g045870 32.201 795 477 19 18 782 11 773 5.26e-122 386
MS.gene22334.t1 MTR_0002s1180 31.325 830 506 19 8 798 2 806 4.27e-120 381
MS.gene22334.t1 MTR_5g067690 31.904 793 491 16 19 782 23 795 1.83e-119 380
MS.gene22334.t1 MTR_5g074340 30.818 782 492 17 18 778 30 783 6.36e-117 373
MS.gene22334.t1 MTR_5g074380 31.836 779 484 16 19 778 76 826 1.15e-116 374
MS.gene22334.t1 MTR_5g074360 31.314 776 492 15 19 778 31 781 3.79e-115 369
MS.gene22334.t1 MTR_3g071990 32.336 702 441 13 87 778 6 683 3.15e-113 361
MS.gene22334.t1 MTR_3g037270 31.117 797 505 19 9 782 17 792 3.96e-111 358
MS.gene22334.t1 MTR_3g071870 30.000 780 493 16 18 767 32 788 3.00e-109 353
MS.gene22334.t1 MTR_3g071880 29.658 789 509 20 18 777 19 790 6.09e-109 352
MS.gene22334.t1 MTR_5g074320 31.862 725 457 14 68 778 10 711 6.77e-109 350
MS.gene22334.t1 MTR_3g037260 30.013 783 514 15 22 782 20 790 9.14e-106 343
MS.gene22334.t1 MTR_3g031160 28.553 788 519 16 10 781 23 782 1.85e-102 334
MS.gene22334.t1 MTR_6g015940 30.337 801 498 20 4 778 19 785 2.46e-97 320
MS.gene22334.t1 MTR_8g085260 29.141 803 513 18 1 778 18 789 1.85e-95 315
MS.gene22334.t1 MTR_4g037755 29.815 758 489 21 49 782 35 773 8.41e-95 313
MS.gene22334.t1 MTR_4g029520 28.016 771 513 18 38 781 25 780 1.36e-93 310
MS.gene22334.t1 MTR_5g088470 28.218 808 511 23 4 778 17 788 1.19e-92 308
MS.gene22334.t1 MTR_3g498715 28.269 803 493 20 9 774 32 788 8.34e-92 306
MS.gene22334.t1 MTR_6g053320 29.741 733 458 20 81 785 69 772 8.36e-92 305
MS.gene22334.t1 MTR_5g088450 28.464 801 521 18 4 778 17 791 1.41e-90 303
MS.gene22334.t1 MTR_3g110700 27.285 766 525 14 38 781 22 777 2.76e-90 301
MS.gene22334.t1 MTR_3g083850 27.633 807 513 19 4 778 17 784 3.49e-89 299
MS.gene22334.t1 MTR_5g088430 28.121 793 510 19 14 778 28 788 2.88e-87 294
MS.gene22334.t1 MTR_7g085870 27.708 794 501 20 25 778 34 794 3.97e-87 294
MS.gene22334.t1 MTR_3g031030 27.974 765 483 18 49 779 35 765 2.39e-84 285
MS.gene22334.t1 MTR_5g088790 28.250 800 520 19 9 778 39 814 1.69e-79 273
MS.gene22334.t1 MTR_5g088840 27.826 805 518 20 9 778 39 815 1.55e-78 270
MS.gene22334.t1 MTR_7g052680 28.144 803 517 22 9 778 39 814 6.77e-78 268
MS.gene22334.t1 MTR_7g053000 27.534 799 527 18 9 778 39 814 9.34e-78 268
MS.gene22334.t1 MTR_7g052970 27.534 799 527 18 9 778 39 814 8.29e-77 265
MS.gene22334.t1 MTR_3g465680 28.329 413 286 4 20 426 44 452 4.82e-51 193
MS.gene22334.t1 MTR_3g465680 30.670 388 232 12 401 778 555 915 5.11e-40 159
MS.gene22334.t1 MTR_7g052895 23.400 547 391 10 9 534 39 578 4.24e-41 161
MS.gene22334.t1 MTR_5g083560 32.817 323 179 9 469 778 1 298 6.11e-38 145
MS.gene22334.t1 MTR_5g061460 56.250 96 42 0 455 550 15 110 2.56e-31 120
MS.gene22334.t1 MTR_1g082340 56.250 96 42 0 455 550 82 177 5.23e-31 122
MS.gene22334.t1 MTR_3g083860 25.000 236 135 7 14 244 28 226 1.29e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22334.t1 AT5G41610 67.087 793 250 7 1 785 1 790 0.0 1050
MS.gene22334.t1 AT5G41610 67.087 793 250 7 1 785 1 790 0.0 1050
MS.gene22334.t1 AT4G23700 61.322 817 296 7 6 802 3 819 0.0 976
MS.gene22334.t1 AT4G23700 61.322 817 296 7 6 802 3 819 0.0 976
MS.gene22334.t1 AT5G41610 67.082 723 229 6 71 785 1 722 0.0 951
MS.gene22334.t1 AT3G17630 61.004 777 297 3 3 779 2 772 0.0 937
MS.gene22334.t1 AT3G17630 61.004 777 297 3 3 779 2 772 0.0 937
MS.gene22334.t1 AT1G64170 56.258 791 312 14 11 779 14 792 0.0 822
MS.gene22334.t1 AT2G13620 47.925 795 382 10 4 779 8 789 0.0 719
MS.gene22334.t1 AT3G53720 50.739 812 353 14 15 781 8 817 0.0 693
MS.gene22334.t1 AT3G53720 49.128 745 332 14 82 781 2 744 0.0 598
MS.gene22334.t1 AT1G05580 35.058 773 472 14 23 779 41 799 3.99e-138 430
MS.gene22334.t1 AT2G31910 33.550 769 489 10 24 779 28 787 1.44e-126 399
MS.gene22334.t1 AT1G05580 35.000 700 427 12 95 779 2 688 1.56e-125 394
MS.gene22334.t1 AT1G06970 31.194 779 505 14 10 778 27 784 1.91e-113 364
MS.gene22334.t1 AT2G31910 33.524 698 442 10 95 779 2 690 7.75e-112 357
MS.gene22334.t1 AT2G31910 33.524 698 442 10 95 779 2 690 7.75e-112 357
MS.gene22334.t1 AT2G30240 30.224 804 527 15 10 800 29 811 8.25e-105 342
MS.gene22334.t1 AT5G58460 27.778 792 521 14 21 778 53 827 3.08e-93 311
MS.gene22334.t1 AT5G37060 27.238 793 526 15 21 778 53 829 2.35e-90 303
MS.gene22334.t1 AT5G22900 27.083 816 519 18 14 778 27 817 1.34e-87 295
MS.gene22334.t1 AT5G01690 27.134 785 523 17 10 778 16 767 3.57e-84 285
MS.gene22334.t1 AT3G44900 27.396 814 525 19 14 783 26 817 1.09e-80 276
MS.gene22334.t1 AT3G52080 27.206 816 521 20 3 778 2 784 2.13e-78 270
MS.gene22334.t1 AT2G28180 28.160 799 515 19 12 778 37 808 1.39e-77 268
MS.gene22334.t1 AT1G08150 28.025 785 491 22 24 772 56 802 2.49e-75 261
MS.gene22334.t1 AT1G08140 27.623 753 482 23 57 778 83 803 7.80e-74 257
MS.gene22334.t1 AT5G01680 26.438 817 516 23 1 778 5 775 9.14e-73 254
MS.gene22334.t1 AT1G79400 27.073 772 536 15 24 778 11 772 3.27e-72 252
MS.gene22334.t1 AT2G28170 26.972 786 514 23 19 778 47 798 1.18e-67 240
MS.gene22334.t1 AT1G16380 24.841 785 528 18 33 781 19 777 5.14e-66 235
MS.gene22334.t1 AT5G22910 26.864 778 490 18 31 768 47 785 1.32e-65 234
MS.gene22334.t1 AT3G44930 25.819 763 514 16 31 769 35 769 5.30e-64 229
MS.gene22334.t1 AT3G44910 24.125 800 529 17 1 767 1 755 4.56e-61 221
MS.gene22334.t1 AT3G44920 24.903 771 525 16 31 776 35 776 9.79e-60 217
MS.gene22334.t1 AT1G08135 25.388 709 463 19 95 778 127 794 8.16e-53 197

Find 186 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATCTTCTAAAACCTTTA+AGG 0.099756 5.4:+4306357 MS.gene22334:CDS
TTCCGCATTTCTTGTATATA+TGG 0.132317 5.4:+4307192 MS.gene22334:CDS
GGTGAATGTTCCTCCATTTC+TGG 0.139824 5.4:-4309176 None:intergenic
ACCTCTCACAAAATTCTTTA+AGG 0.140574 5.4:-4308281 None:intergenic
TGATCAGACCTTTGCTATTA+TGG 0.150282 5.4:+4308023 MS.gene22334:CDS
GCTCTTGCTGTTGCCTTATC+AGG 0.189626 5.4:+4307349 MS.gene22334:CDS
GACAAAATGGGTTGCCGTTT+TGG 0.198490 5.4:+4308334 MS.gene22334:CDS
TCCTGTATTAGCCCGTATTT+TGG 0.201280 5.4:+4307240 MS.gene22334:CDS
ATGACTCGAGGTTGCCTTAA+AGG 0.205612 5.4:-4306371 None:intergenic
GTGATCTGTCCTACAATATT+AGG 0.206047 5.4:-4308804 None:intergenic
GTTACATTTACGGCTTGTTT+TGG 0.223359 5.4:+4307763 MS.gene22334:CDS
ATTTCTGGGACCAAGTTTAT+TGG 0.243090 5.4:-4309161 None:intergenic
GCACAAAAGGTAAGACAAAA+TGG 0.243930 5.4:+4308321 MS.gene22334:CDS
CCGGCAAACGCTCCATCTTT+TGG 0.270031 5.4:-4307613 None:intergenic
TTAGCTGCTGTTCTGGCTGC+TGG 0.279693 5.4:+4307526 MS.gene22334:CDS
CCGATGCAGCCTGCGTCTAA+TGG 0.279802 5.4:+4306245 MS.gene22334:CDS
TGGGCCATCCATTTGAAAAT+TGG 0.287723 5.4:-4307454 None:intergenic
TGCTACATTAGCTGCTGTTC+TGG 0.289882 5.4:+4307519 MS.gene22334:CDS
CCGCACAACAGCATCTGTTT+TGG 0.302658 5.4:+4309112 MS.gene22334:CDS
CCTAAATCTCTGCGGAAAAC+TGG 0.313847 5.4:+4307064 MS.gene22334:CDS
AGAAGCGAGTGCGCGGAATT+AGG 0.315072 5.4:+4309056 MS.gene22334:CDS
GACCAACATAGCTACCATTC+AGG 0.327011 5.4:+4307711 MS.gene22334:CDS
ATCGGAATTCATGCCATGTT+TGG 0.328149 5.4:+4307565 MS.gene22334:CDS
GACCGTGAAGCTCTTGCTTA+TGG 0.329784 5.4:+4308723 MS.gene22334:CDS
GTCCTTGCCATTGCTATTGC+CGG 0.332317 5.4:+4307094 MS.gene22334:CDS
TCCGCATTTCTTGTATATAT+GGG 0.333877 5.4:+4307193 MS.gene22334:CDS
CCATTCAGGGGCTGCAGTCA+TGG 0.338451 5.4:+4307725 MS.gene22334:CDS
TTACCCTTTGCACTAGGAAT+TGG 0.338535 5.4:+4307124 MS.gene22334:CDS
TTATTGGAACCTAATATTGT+AGG 0.354215 5.4:+4308795 MS.gene22334:CDS
AACCTTTGTTGCTTATGAAA+TGG 0.354534 5.4:-4308525 None:intergenic
TTCTGCAACTTATTTCTTGT+GGG 0.359669 5.4:+4308996 MS.gene22334:CDS
TGGTTCATCGTTTGTTCTAC+AGG 0.363937 5.4:+4307144 MS.gene22334:CDS
TGCGGAAGTATTTACACCTT+TGG 0.366289 5.4:-4307176 None:intergenic
GCTCACCTTCATGGCATTGT+TGG 0.366554 5.4:-4307474 None:intergenic
GTGAATGTTCCTCCATTTCT+GGG 0.373379 5.4:-4309175 None:intergenic
AAAATAGAGGATCTTGTATC+TGG 0.375448 5.4:+4307649 MS.gene22334:CDS
CTCACCTTCATGGCATTGTT+GGG 0.377633 5.4:-4307473 None:intergenic
ATCTCCATTTGTGTCATTGT+GGG 0.381610 5.4:+4307381 MS.gene22334:CDS
CATCAACCGGCTCACCTTCA+TGG 0.381935 5.4:-4307483 None:intergenic
CATTCAGGGGCTGCAGTCAT+GGG 0.383910 5.4:+4307726 MS.gene22334:CDS
AACATTTCGAGCTGTGGTAT+TGG 0.387586 5.4:-4306213 None:intergenic
ATTGATGAAATTGCTATCAT+TGG 0.392049 5.4:-4308438 None:intergenic
CCAAAAGATGGAGCGTTTGC+CGG 0.392899 5.4:+4307613 MS.gene22334:CDS
TCGAAGGCCGGACGCTGAAT+TGG 0.398474 5.4:+4309019 MS.gene22334:CDS
TAATCTTATGCCAGGGTGTT+CGG 0.399773 5.4:-4308757 None:intergenic
AGGATTCTTGCCTGTTTCCA+TGG 0.404187 5.4:+4308171 MS.gene22334:CDS
GAAAAGCACAACAATGCAAT+AGG 0.404326 5.4:-4308685 None:intergenic
ATCTAATACTGGAAGACTCT+TGG 0.407831 5.4:-4306984 None:intergenic
CCATGTTATTGTGGCGTTCG+AGG 0.411384 5.4:+4308383 MS.gene22334:CDS
TTGCCTGTTTCCATGGTTCA+AGG 0.413150 5.4:+4308178 MS.gene22334:CDS
TTTCTGCAACTTATTTCTTG+TGG 0.414228 5.4:+4308995 MS.gene22334:CDS
TGCAAAGGGTAAGCTTATTC+CGG 0.415247 5.4:-4307113 None:intergenic
CTACTTGGGCCGTCAGCGCT+TGG 0.415516 5.4:+4306926 MS.gene22334:CDS
AATAAACTTGGTCCCAGAAA+TGG 0.416719 5.4:+4309163 MS.gene22334:CDS
ATTCCTTGAACCATGGAAAC+AGG 0.420826 5.4:-4308181 None:intergenic
TTTGCTAGAGTATCTAATAC+TGG 0.434840 5.4:-4306995 None:intergenic
CAATGGGGTGCCAATAAACT+TGG 0.442323 5.4:+4309151 MS.gene22334:CDS
GACGCAGGCTGCATCGGCGG+CGG 0.445153 5.4:-4306239 None:intergenic
TCTAATACTGGAAGACTCTT+GGG 0.445615 5.4:-4306983 None:intergenic
CCGCATTTCTTGTATATATG+GGG 0.447740 5.4:+4307194 MS.gene22334:CDS
GCCAAAATACGGGCTAATAC+AGG 0.448964 5.4:-4307241 None:intergenic
ATTTCTTGTGGGTCGAAGGC+CGG 0.449582 5.4:+4309007 MS.gene22334:CDS
ATCGATACTAATGGCACAAA+AGG 0.453823 5.4:+4308308 MS.gene22334:CDS
GCGTCTAATGGTGTATTTCA+AGG 0.458325 5.4:+4306257 MS.gene22334:CDS
ACACCCTGGCATAAGATTAG+TGG 0.462991 5.4:+4308761 MS.gene22334:CDS
TAGCTGCTGTTCTGGCTGCT+GGG 0.463429 5.4:+4307527 MS.gene22334:CDS
CCCCTGAATGGTAGCTATGT+TGG 0.463640 5.4:-4307713 None:intergenic
TTGTGTTAGCTGCATATAAG+AGG 0.463707 5.4:+4308085 MS.gene22334:CDS
ACGCAGGCTGCATCGGCGGC+GGG 0.465545 5.4:-4306238 None:intergenic
TTTGATGAATAGTAAAGGCT+TGG 0.466519 5.4:+4307858 MS.gene22334:CDS
TCATTGTGGGTCTTCTTAAG+TGG 0.468755 5.4:+4307394 MS.gene22334:CDS
ATCGATGATAAACTTGATTG+AGG 0.470338 5.4:+4308209 MS.gene22334:CDS
TTGAACGAGTCTTTAGTGCT+TGG 0.470752 5.4:+4307832 MS.gene22334:CDS
TCTTGCAGTCACAAGGGGAC+TGG 0.473223 5.4:+4306334 MS.gene22334:CDS
TTTGTTGATCGCGGGCTTGG+TGG 0.473988 5.4:+4308633 MS.gene22334:CDS
AAGACCCACAATGACACAAA+TGG 0.479162 5.4:-4307385 None:intergenic
GCAAAGGCCAATTCAGCGTC+CGG 0.480318 5.4:-4309026 None:intergenic
TGACAGGGAGGAATCCTACT+TGG 0.484231 5.4:+4306911 MS.gene22334:intron
AATGATAGCAATTTCATCAA+TGG 0.488394 5.4:+4308440 MS.gene22334:CDS
AGGACCTGTTGGTGGCTTGT+TGG 0.488446 5.4:+4309076 MS.gene22334:CDS
GTCTTGTTCTTGCAGTCACA+AGG 0.489013 5.4:+4306327 MS.gene22334:CDS
GAAGATTCCGTGTCTCCATC+AGG 0.489657 5.4:-4309197 None:intergenic
AGCAAGAGCAAGAAGTATCC+AGG 0.491761 5.4:-4307335 None:intergenic
CATATGTATAACTCATCAAC+CGG 0.492318 5.4:-4307496 None:intergenic
AGATTGGCTATTGCCTGATA+AGG 0.492432 5.4:-4307362 None:intergenic
CAACATCGGTAAAGATAGAA+AGG 0.492937 5.4:+4307897 MS.gene22334:CDS
CTCCATAAGCAAGAGCTTCA+CGG 0.493215 5.4:-4308725 None:intergenic
CCATGACTGCAGCCCCTGAA+TGG 0.495906 5.4:-4307725 None:intergenic
CCACTTGACACAAAATAAAG+TGG 0.501014 5.4:-4307682 None:intergenic
AGAGTCTCCAACATCAACTT+TGG 0.501856 5.4:-4308826 None:intergenic
GATGCCAACAAGCCACCAAC+AGG 0.505963 5.4:-4309080 None:intergenic
AATCTCCATTTGTGTCATTG+TGG 0.506174 5.4:+4307380 MS.gene22334:CDS
TGGGTCTTCTTAAGTGGGTG+TGG 0.508408 5.4:+4307400 MS.gene22334:CDS
GAACCAATTCCTAGTGCAAA+GGG 0.508620 5.4:-4307127 None:intergenic
TTCTATCTTTACCGATGTTG+AGG 0.509100 5.4:-4307894 None:intergenic
TCAATCAAGTTTATCATCGA+TGG 0.509658 5.4:-4308207 None:intergenic
ACTTATTTCTTGTGGGTCGA+AGG 0.511823 5.4:+4309003 MS.gene22334:CDS
CATAAGCAACAAAGGTTAGA+CGG 0.512409 5.4:+4308531 MS.gene22334:CDS
TCTTGATTATGCACTTCCAT+TGG 0.513464 5.4:+4306289 MS.gene22334:CDS
AAAACCACTAATCTTATGCC+AGG 0.514861 5.4:-4308765 None:intergenic
CATTGTGGGTCTTCTTAAGT+GGG 0.515396 5.4:+4307395 MS.gene22334:CDS
CACAAAAGGTAAGACAAAAT+GGG 0.518007 5.4:+4308322 MS.gene22334:CDS
TTATTCCGTCCAAGCGCTGA+CGG 0.519020 5.4:-4306935 None:intergenic
CTCGCTTCTTTCAAGAGCAA+AGG 0.521580 5.4:-4309042 None:intergenic
CGCATTTCTTGTATATATGG+GGG 0.521611 5.4:+4307195 MS.gene22334:CDS
ACAATGACACAAATGGAGAT+TGG 0.521930 5.4:-4307378 None:intergenic
CCATTAGACGCAGGCTGCAT+CGG 0.522930 5.4:-4306245 None:intergenic
TCTTGCTTATGGAGCTCGTA+TGG 0.526592 5.4:+4308734 MS.gene22334:CDS
TTCCATTTCATAAGCAACAA+AGG 0.530602 5.4:+4308523 MS.gene22334:CDS
TTGGGCCGTCAGCGCTTGGA+CGG 0.534153 5.4:+4306930 MS.gene22334:CDS
GGGTTTGTCACGGATGCTAT+CGG 0.536342 5.4:+4307547 MS.gene22334:CDS
ATAAGCTTACCCTTTGCACT+AGG 0.536559 5.4:+4307118 MS.gene22334:CDS
ATAGATAATTGAGTCATCAC+TGG 0.537461 5.4:-4308916 None:intergenic
TCTGGCTGCTGGGTTTGTCA+CGG 0.539409 5.4:+4307537 MS.gene22334:CDS
ACCAACATAGCTACCATTCA+GGG 0.542167 5.4:+4307712 MS.gene22334:CDS
CAGATCACCAAAGTTGATGT+TGG 0.543622 5.4:+4308819 MS.gene22334:CDS
ATGGAATATTCCTTGAACCA+TGG 0.546940 5.4:-4308188 None:intergenic
TGATGCAACAGTATCACAAT+GGG 0.550837 5.4:+4309135 MS.gene22334:CDS
TGGTGACCTTTATCCCAAAA+CGG 0.553372 5.4:-4308348 None:intergenic
TGCTCTTGTGGAGAAAATAG+AGG 0.555546 5.4:+4307636 MS.gene22334:CDS
GAGCATGCACCATGCTCAGT+TGG 0.559655 5.4:+4308606 MS.gene22334:CDS
CAAGCGCTGACGGCCCAAGT+AGG 0.564029 5.4:-4306925 None:intergenic
CAGTTAATGATGTAGCAGCC+TGG 0.564394 5.4:+4307317 MS.gene22334:CDS
ATGGAGGAACATTCACCTGA+TGG 0.565713 5.4:+4309182 MS.gene22334:CDS
TGAAAATTGGAAGGACAATG+AGG 0.565770 5.4:-4307441 None:intergenic
AACCACTAGTGTCGGCAGAA+TGG 0.566373 5.4:+4307279 MS.gene22334:CDS
CATATCTGTAAAATAGCCAA+TGG 0.568781 5.4:-4306305 None:intergenic
CACAAGAGGAGTGGTCATGA+AGG 0.569719 5.4:-4308067 None:intergenic
CCATCCATTTGAAAATTGGA+AGG 0.571739 5.4:-4307450 None:intergenic
CCTCGAACGCCACAATAACA+TGG 0.571844 5.4:-4308383 None:intergenic
AGCACTAAAGACTCGTTCAA+AGG 0.572987 5.4:-4307829 None:intergenic
GCGGAATTAGGACCTGTTGG+TGG 0.575797 5.4:+4309068 MS.gene22334:CDS
CTTGTTCTTGCAGTCACAAG+GGG 0.579341 5.4:+4306329 MS.gene22334:CDS
TGCGCGGAATTAGGACCTGT+TGG 0.580143 5.4:+4309065 MS.gene22334:CDS
GCTCGTATGGCCGAACACCC+TGG 0.581362 5.4:+4308747 MS.gene22334:CDS
GGTTCATCGTTTGTTCTACA+GGG 0.584720 5.4:+4307145 MS.gene22334:CDS
ATTCAGGGGCTGCAGTCATG+GGG 0.586038 5.4:+4307727 MS.gene22334:CDS
CGGGCAAACATTTCGAGCTG+TGG 0.586544 5.4:-4306219 None:intergenic
ACAATGCCATGAAGGTGAGC+CGG 0.586955 5.4:+4307477 MS.gene22334:CDS
AGCGTTTGCCGGTGCTCTTG+TGG 0.587306 5.4:+4307624 MS.gene22334:CDS
CATGAAAACCATAATAGCAA+AGG 0.589901 5.4:-4308031 None:intergenic
GAATTAATTGTCCTCAACAT+CGG 0.591363 5.4:+4307883 MS.gene22334:CDS
GAGGTCCTCATCGATACTAA+TGG 0.592793 5.4:+4308299 MS.gene22334:CDS
TCTTGTTCTTGCAGTCACAA+GGG 0.592967 5.4:+4306328 MS.gene22334:CDS
TTAGACGCAGGCTGCATCGG+CGG 0.594119 5.4:-4306242 None:intergenic
GTGATGCAACAGTATCACAA+TGG 0.594247 5.4:+4309134 MS.gene22334:CDS
AACAGGCAAGAATCCTCAGT+TGG 0.594260 5.4:-4308164 None:intergenic
TTAGATACTCTAGCAAACCT+TGG 0.598078 5.4:+4307001 MS.gene22334:CDS
TGAACCAATTCCTAGTGCAA+AGG 0.603458 5.4:-4307128 None:intergenic
ACAAATACAGAACAATTGAA+AGG 0.605182 5.4:+4308127 MS.gene22334:CDS
TTATATGCAGCTAACACAAG+AGG 0.606611 5.4:-4308081 None:intergenic
TTCCGGCAATAGCAATGGCA+AGG 0.606740 5.4:-4307096 None:intergenic
GCTTATTCCGGCAATAGCAA+TGG 0.606834 5.4:-4307101 None:intergenic
AACTTGATTGAGGCTTCAAG+AGG 0.612831 5.4:+4308219 MS.gene22334:CDS
AAACTTGGTCCCAGAAATGG+AGG 0.614749 5.4:+4309166 MS.gene22334:CDS
GACAGGGAGGAATCCTACTT+GGG 0.614800 5.4:+4306912 MS.gene22334:intron
AATACTGGAAGACTCTTGGG+TGG 0.618474 5.4:-4306980 None:intergenic
AGATTCTGTCCATGTTATTG+TGG 0.620026 5.4:+4308374 MS.gene22334:CDS
AAACTTCTAACCACTAGTGT+CGG 0.620589 5.4:+4307271 MS.gene22334:CDS
ATGGCCCAACAATGCCATGA+AGG 0.621341 5.4:+4307469 MS.gene22334:CDS
TAACATGGACAGAATCTCCA+TGG 0.622510 5.4:-4308368 None:intergenic
GCAGAAATTAATCACGCGGA+TGG 0.623209 5.4:-4308979 None:intergenic
CACTTGACACAAAATAAAGT+GGG 0.625867 5.4:-4307681 None:intergenic
TTGTGCCATTAGTATCGATG+AGG 0.638116 5.4:-4308304 None:intergenic
CCAAAACAGATGCTGTTGTG+CGG 0.640724 5.4:-4309112 None:intergenic
AAAATGCAGACGGCCAACTG+AGG 0.641897 5.4:+4308151 MS.gene22334:CDS
AATGGAAGTGCATAATCAAG+AGG 0.642363 5.4:-4306287 None:intergenic
AGCCATTCTGCCGACACTAG+TGG 0.642910 5.4:-4307281 None:intergenic
AAATCTCTGCGGAAAACTGG+AGG 0.646572 5.4:+4307067 MS.gene22334:CDS
ATACTGGAAGACTCTTGGGT+GGG 0.647325 5.4:-4306979 None:intergenic
AAACCACTAATCTTATGCCA+GGG 0.648115 5.4:-4308764 None:intergenic
TCTTGAAAGAAGCGAGTGCG+CGG 0.650120 5.4:+4309049 MS.gene22334:CDS
ACATTCACCTGATGGAGACA+CGG 0.653891 5.4:+4309190 MS.gene22334:CDS
CCCCATATATACAAGAAATG+CGG 0.658156 5.4:-4307194 None:intergenic
TGCAGCTAACACAAGAGGAG+TGG 0.658853 5.4:-4308076 None:intergenic
ACAATCTCTGCAATGACTCG+AGG 0.658940 5.4:-4306383 None:intergenic
CTACAGGGAACAATTGCCAA+AGG 0.662191 5.4:+4307160 MS.gene22334:CDS
ATGGAAGTGCATAATCAAGA+GGG 0.669234 5.4:-4306286 None:intergenic
ATAGAAAGAATATAAGACCA+AGG 0.670462 5.4:-4307018 None:intergenic
TGAAATACACCATTAGACGC+AGG 0.678423 5.4:-4306254 None:intergenic
AGTTGCAGAAATTAATCACG+CGG 0.687377 5.4:-4308983 None:intergenic
TCATTGGTCGAACACACACG+TGG 0.711445 5.4:-4308422 None:intergenic
AGTTAGATCCTAAATCTCTG+CGG 0.716660 5.4:+4307056 MS.gene22334:CDS
CCAACATAGCTACCATTCAG+GGG 0.726005 5.4:+4307713 MS.gene22334:CDS
AAATGGAAGAATGATGACAG+CGG 0.728068 5.4:-4308508 None:intergenic
GATGCAACAGTATCACAATG+GGG 0.828892 5.4:+4309136 MS.gene22334:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCCTATAATTAATTTAAAA+TGG + chr5.4:4307950-4307969 MS.gene22334:intron 10.0%
!! AATTAATTATAGGAATAATG+AGG - chr5.4:4307945-4307964 None:intergenic 15.0%
!! ACTGTGAAATTATATAAAAA+TGG - chr5.4:4306562-4306581 None:intergenic 15.0%
!! TTTATATATTAGAAACTAGT+GGG + chr5.4:4306496-4306515 MS.gene22334:intron 15.0%
!!! CACCATTTTAAATTAATTAT+AGG - chr5.4:4307955-4307974 None:intergenic 15.0%
!!! TTTTATATATTAGAAACTAG+TGG + chr5.4:4306495-4306514 MS.gene22334:intron 15.0%
!! TTATATATTAGAAACTAGTG+GGG + chr5.4:4306497-4306516 MS.gene22334:intron 20.0%
!!! ATATTCTTTCTATTTTTAGC+AGG + chr5.4:4307028-4307047 MS.gene22334:CDS 20.0%
! AACTTCATTAAATCCACATT+TGG + chr5.4:4306803-4306822 MS.gene22334:intron 25.0%
! AATGATAGCAATTTCATCAA+TGG + chr5.4:4308440-4308459 MS.gene22334:CDS 25.0%
! ACAAATACAGAACAATTGAA+AGG + chr5.4:4308127-4308146 MS.gene22334:CDS 25.0%
! ATAGAAAGAATATAAGACCA+AGG - chr5.4:4307021-4307040 None:intergenic 25.0%
! ATATAAAAATGGAACACTCA+AGG - chr5.4:4306551-4306570 None:intergenic 25.0%
! ATTGATGAAATTGCTATCAT+TGG - chr5.4:4308441-4308460 None:intergenic 25.0%
! TTATTGGAACCTAATATTGT+AGG + chr5.4:4308795-4308814 MS.gene22334:CDS 25.0%
!! GGATTTTTGATGAATAGTAA+AGG + chr5.4:4307853-4307872 MS.gene22334:CDS 25.0%
!! TATAAGAAACGATTTTCGAT+TGG + chr5.4:4308566-4308585 MS.gene22334:CDS 25.0%
!! TTAGAACTTGTTTCATAGTT+TGG - chr5.4:4306612-4306631 None:intergenic 25.0%
!!! ATTTTCGATTGGTTAACAAA+AGG + chr5.4:4308577-4308596 MS.gene22334:CDS 25.0%
!!! TCTAGTTTTCGTTACATTTA+CGG + chr5.4:4307753-4307772 MS.gene22334:CDS 25.0%
!!! TTTTCGATTGGTTAACAAAA+GGG + chr5.4:4308578-4308597 MS.gene22334:CDS 25.0%
AAAATAGAGGATCTTGTATC+TGG + chr5.4:4307649-4307668 MS.gene22334:CDS 30.0%
ACCTCTCACAAAATTCTTTA+AGG - chr5.4:4308284-4308303 None:intergenic 30.0%
ATAGATAATTGAGTCATCAC+TGG - chr5.4:4308919-4308938 None:intergenic 30.0%
ATCGATGATAAACTTGATTG+AGG + chr5.4:4308209-4308228 MS.gene22334:CDS 30.0%
ATTGAAAGGAAAAATGCAGA+CGG + chr5.4:4308141-4308160 MS.gene22334:CDS 30.0%
CAAATATACATCACGATGAT+CGG - chr5.4:4307979-4307998 None:intergenic 30.0%
CACAAAAGGTAAGACAAAAT+GGG + chr5.4:4308322-4308341 MS.gene22334:CDS 30.0%
CACTTGACACAAAATAAAGT+GGG - chr5.4:4307684-4307703 None:intergenic 30.0%
CATATCTGTAAAATAGCCAA+TGG - chr5.4:4306308-4306327 None:intergenic 30.0%
CATATGTATAACTCATCAAC+CGG - chr5.4:4307499-4307518 None:intergenic 30.0%
CATGAAAACCATAATAGCAA+AGG - chr5.4:4308034-4308053 None:intergenic 30.0%
GAATTAATTGTCCTCAACAT+CGG + chr5.4:4307883-4307902 MS.gene22334:CDS 30.0%
TCAATCAAGTTTATCATCGA+TGG - chr5.4:4308210-4308229 None:intergenic 30.0%
TCCGCATTTCTTGTATATAT+GGG + chr5.4:4307193-4307212 MS.gene22334:CDS 30.0%
TTCCATTTCATAAGCAACAA+AGG + chr5.4:4308523-4308542 MS.gene22334:CDS 30.0%
TTCCGCATTTCTTGTATATA+TGG + chr5.4:4307192-4307211 MS.gene22334:CDS 30.0%
TTTGATGAATAGTAAAGGCT+TGG + chr5.4:4307858-4307877 MS.gene22334:CDS 30.0%
! AACCTTTGTTGCTTATGAAA+TGG - chr5.4:4308528-4308547 None:intergenic 30.0%
! ACCTTAAAGAATTTTGTGAG+AGG + chr5.4:4308280-4308299 MS.gene22334:CDS 30.0%
! TATTTGATTTACGTATCTGC+AGG + chr5.4:4307993-4308012 MS.gene22334:intron 30.0%
! TCTTAAACATGTGCACATTT+TGG - chr5.4:4306732-4306751 None:intergenic 30.0%
! TTCTGCAACTTATTTCTTGT+GGG + chr5.4:4308996-4309015 MS.gene22334:CDS 30.0%
! TTGTCCTTCCAATTTTCAAA+TGG + chr5.4:4307446-4307465 MS.gene22334:CDS 30.0%
! TTTCTGCAACTTATTTCTTG+TGG + chr5.4:4308995-4309014 MS.gene22334:CDS 30.0%
! TTTGCTAGAGTATCTAATAC+TGG - chr5.4:4306998-4307017 None:intergenic 30.0%
!! ATTGCTTATTGATGATGACA+GGG + chr5.4:4306896-4306915 MS.gene22334:intron 30.0%
!! CCTATCTTCTAAAACCTTTA+AGG + chr5.4:4306357-4306376 MS.gene22334:CDS 30.0%
!! TTACATTTACGGCTTGTTTT+GGG + chr5.4:4307764-4307783 MS.gene22334:CDS 30.0%
!!! AGTGGTTAGAAGTTTTAACT+CGG - chr5.4:4307266-4307285 None:intergenic 30.0%
!!! CCTTAAAGGTTTTAGAAGAT+AGG - chr5.4:4306360-4306379 None:intergenic 30.0%
!!! CTTTGCTATTATGGTTTTCA+TGG + chr5.4:4308032-4308051 MS.gene22334:CDS 30.0%
AAAACCACTAATCTTATGCC+AGG - chr5.4:4308768-4308787 None:intergenic 35.0%
AAACCACTAATCTTATGCCA+GGG - chr5.4:4308767-4308786 None:intergenic 35.0%
AAACTTCTAACCACTAGTGT+CGG + chr5.4:4307271-4307290 MS.gene22334:CDS 35.0%
AAATGGAAGAATGATGACAG+CGG - chr5.4:4308511-4308530 None:intergenic 35.0%
AATAAACTTGGTCCCAGAAA+TGG + chr5.4:4309163-4309182 MS.gene22334:CDS 35.0%
ACAAAAATTCCAACTGAGCA+TGG - chr5.4:4308618-4308637 None:intergenic 35.0%
ACAATGACACAAATGGAGAT+TGG - chr5.4:4307381-4307400 None:intergenic 35.0%
AGATTCTGTCCATGTTATTG+TGG + chr5.4:4308374-4308393 MS.gene22334:CDS 35.0%
AGTTAGATCCTAAATCTCTG+CGG + chr5.4:4307056-4307075 MS.gene22334:CDS 35.0%
AGTTGCAGAAATTAATCACG+CGG - chr5.4:4308986-4309005 None:intergenic 35.0%
ATCGATACTAATGGCACAAA+AGG + chr5.4:4308308-4308327 MS.gene22334:CDS 35.0%
ATCTAATACTGGAAGACTCT+TGG - chr5.4:4306987-4307006 None:intergenic 35.0%
ATGGAATATTCCTTGAACCA+TGG - chr5.4:4308191-4308210 None:intergenic 35.0%
CAACATCGGTAAAGATAGAA+AGG + chr5.4:4307897-4307916 MS.gene22334:CDS 35.0%
CATAAGCAACAAAGGTTAGA+CGG + chr5.4:4308531-4308550 MS.gene22334:CDS 35.0%
CCACTTGACACAAAATAAAG+TGG - chr5.4:4307685-4307704 None:intergenic 35.0%
CCATCCATTTGAAAATTGGA+AGG - chr5.4:4307453-4307472 None:intergenic 35.0%
CCCCATATATACAAGAAATG+CGG - chr5.4:4307197-4307216 None:intergenic 35.0%
CCGCATTTCTTGTATATATG+GGG + chr5.4:4307194-4307213 MS.gene22334:CDS 35.0%
CGCATTTCTTGTATATATGG+GGG + chr5.4:4307195-4307214 MS.gene22334:CDS 35.0%
GCACAAAAGGTAAGACAAAA+TGG + chr5.4:4308321-4308340 MS.gene22334:CDS 35.0%
GTGATCTGTCCTACAATATT+AGG - chr5.4:4308807-4308826 None:intergenic 35.0%
TCTAATACTGGAAGACTCTT+GGG - chr5.4:4306986-4307005 None:intergenic 35.0%
TCTTGATTATGCACTTCCAT+TGG + chr5.4:4306289-4306308 MS.gene22334:CDS 35.0%
TGAAAATTGGAAGGACAATG+AGG - chr5.4:4307444-4307463 None:intergenic 35.0%
TGATGCAACAGTATCACAAT+GGG + chr5.4:4309135-4309154 MS.gene22334:CDS 35.0%
TTAGAAACTAGTGGGGAAAT+TGG + chr5.4:4306504-4306523 MS.gene22334:intron 35.0%
TTAGATACTCTAGCAAACCT+TGG + chr5.4:4307001-4307020 MS.gene22334:CDS 35.0%
TTAGTAAGACTGACCAAATG+TGG - chr5.4:4306819-4306838 None:intergenic 35.0%
TTATATGCAGCTAACACAAG+AGG - chr5.4:4308084-4308103 None:intergenic 35.0%
TTCTATCTTTACCGATGTTG+AGG - chr5.4:4307897-4307916 None:intergenic 35.0%
TTGTGTTAGCTGCATATAAG+AGG + chr5.4:4308085-4308104 MS.gene22334:CDS 35.0%
! AATCTCCATTTGTGTCATTG+TGG + chr5.4:4307380-4307399 MS.gene22334:CDS 35.0%
! AATGGAAGTGCATAATCAAG+AGG - chr5.4:4306290-4306309 None:intergenic 35.0%
! AGTGGTTTTTCGCTTCTTAT+TGG + chr5.4:4308779-4308798 MS.gene22334:CDS 35.0%
! ATCTCCATTTGTGTCATTGT+GGG + chr5.4:4307381-4307400 MS.gene22334:CDS 35.0%
! ATGGAAGTGCATAATCAAGA+GGG - chr5.4:4306289-4306308 None:intergenic 35.0%
! ATTGTTGTGCTTTTCTTTGG+TGG + chr5.4:4308693-4308712 MS.gene22334:CDS 35.0%
! CCTTCCAATTTTCAAATGGA+TGG + chr5.4:4307450-4307469 MS.gene22334:CDS 35.0%
! GAAAAGCACAACAATGCAAT+AGG - chr5.4:4308688-4308707 None:intergenic 35.0%
! GTTGGAATTTTTGTTGATCG+CGG + chr5.4:4308624-4308643 MS.gene22334:CDS 35.0%
! TGCATTGTTGTGCTTTTCTT+TGG + chr5.4:4308690-4308709 MS.gene22334:CDS 35.0%
! TTGGAATTTTTGTTGATCGC+GGG + chr5.4:4308625-4308644 MS.gene22334:CDS 35.0%
!! AACCTCTGCTCTATTTTAGA+AGG - chr5.4:4306845-4306864 None:intergenic 35.0%
!! ATTTCTGGGACCAAGTTTAT+TGG - chr5.4:4309164-4309183 None:intergenic 35.0%
!! GATTGCTTATTGATGATGAC+AGG + chr5.4:4306895-4306914 MS.gene22334:intron 35.0%
!! GTTACATTTACGGCTTGTTT+TGG + chr5.4:4307763-4307782 MS.gene22334:CDS 35.0%
!! TGATCAGACCTTTGCTATTA+TGG + chr5.4:4308023-4308042 MS.gene22334:CDS 35.0%
!! TTTTAACTCGGCCAAAATAC+GGG - chr5.4:4307254-4307273 None:intergenic 35.0%
!!! CCACTTTATTTTGTGTCAAG+TGG + chr5.4:4307682-4307701 MS.gene22334:CDS 35.0%
!!! CGCGTTTGTTTTTGGAATTT+TGG + chr5.4:4307588-4307607 MS.gene22334:CDS 35.0%
!!! CTTGTTTTGGGAAGATTGTT+GGG + chr5.4:4307776-4307795 MS.gene22334:CDS 35.0%
!!! GGAATTTTGGTTCCAAAAGA+TGG + chr5.4:4307601-4307620 MS.gene22334:CDS 35.0%
!!! GTTTTAACTCGGCCAAAATA+CGG - chr5.4:4307255-4307274 None:intergenic 35.0%
AAAAACAAACGCGCCAAACA+TGG - chr5.4:4307581-4307600 None:intergenic 40.0%
AACATTTCGAGCTGTGGTAT+TGG - chr5.4:4306216-4306235 None:intergenic 40.0%
AACTTGATTGAGGCTTCAAG+AGG + chr5.4:4308219-4308238 MS.gene22334:CDS 40.0%
AAGACCCACAATGACACAAA+TGG - chr5.4:4307388-4307407 None:intergenic 40.0%
ACCAACATAGCTACCATTCA+GGG + chr5.4:4307712-4307731 MS.gene22334:CDS 40.0%
AGAGTCTCCAACATCAACTT+TGG - chr5.4:4308829-4308848 None:intergenic 40.0%
AGCACTAAAGACTCGTTCAA+AGG - chr5.4:4307832-4307851 None:intergenic 40.0%
ATAAGCTTACCCTTTGCACT+AGG + chr5.4:4307118-4307137 MS.gene22334:CDS 40.0%
ATCGGAATTCATGCCATGTT+TGG + chr5.4:4307565-4307584 MS.gene22334:CDS 40.0%
ATTCCTTGAACCATGGAAAC+AGG - chr5.4:4308184-4308203 None:intergenic 40.0%
CATTGTGGGTCTTCTTAAGT+GGG + chr5.4:4307395-4307414 MS.gene22334:CDS 40.0%
GAACCAATTCCTAGTGCAAA+GGG - chr5.4:4307130-4307149 None:intergenic 40.0%
GATGCAACAGTATCACAATG+GGG + chr5.4:4309136-4309155 MS.gene22334:CDS 40.0%
GCAGAGAGTTTAAACACACT+TGG + chr5.4:4306780-4306799 MS.gene22334:intron 40.0%
GCGTCTAATGGTGTATTTCA+AGG + chr5.4:4306257-4306276 MS.gene22334:CDS 40.0%
GGTTCATCGTTTGTTCTACA+GGG + chr5.4:4307145-4307164 MS.gene22334:CDS 40.0%
GTGAATGTTCCTCCATTTCT+GGG - chr5.4:4309178-4309197 None:intergenic 40.0%
GTGATGCAACAGTATCACAA+TGG + chr5.4:4309134-4309153 MS.gene22334:CDS 40.0%
TAACATGGACAGAATCTCCA+TGG - chr5.4:4308371-4308390 None:intergenic 40.0%
TCATTGTGGGTCTTCTTAAG+TGG + chr5.4:4307394-4307413 MS.gene22334:CDS 40.0%
TCTTGTTCTTGCAGTCACAA+GGG + chr5.4:4306328-4306347 MS.gene22334:CDS 40.0%
TGAAATACACCATTAGACGC+AGG - chr5.4:4306257-4306276 None:intergenic 40.0%
TGAACCAATTCCTAGTGCAA+AGG - chr5.4:4307131-4307150 None:intergenic 40.0%
TGCAAAGGGTAAGCTTATTC+CGG - chr5.4:4307116-4307135 None:intergenic 40.0%
TGCTCTTGTGGAGAAAATAG+AGG + chr5.4:4307636-4307655 MS.gene22334:CDS 40.0%
TGGGCCATCCATTTGAAAAT+TGG - chr5.4:4307457-4307476 None:intergenic 40.0%
TGGTGACCTTTATCCCAAAA+CGG - chr5.4:4308351-4308370 None:intergenic 40.0%
TGGTTCATCGTTTGTTCTAC+AGG + chr5.4:4307144-4307163 MS.gene22334:CDS 40.0%
TTACCCTTTGCACTAGGAAT+TGG + chr5.4:4307124-4307143 MS.gene22334:CDS 40.0%
TTGAACGAGTCTTTAGTGCT+TGG + chr5.4:4307832-4307851 MS.gene22334:CDS 40.0%
! ACTTATTTCTTGTGGGTCGA+AGG + chr5.4:4309003-4309022 MS.gene22334:CDS 40.0%
! AGATTGGCTATTGCCTGATA+AGG - chr5.4:4307365-4307384 None:intergenic 40.0%
! TAATCTTATGCCAGGGTGTT+CGG - chr5.4:4308760-4308779 None:intergenic 40.0%
! TCCTGTATTAGCCCGTATTT+TGG + chr5.4:4307240-4307259 MS.gene22334:CDS 40.0%
!! ATGTTTGGCGCGTTTGTTTT+TGG + chr5.4:4307580-4307599 MS.gene22334:CDS 40.0%
!! CAGATCACCAAAGTTGATGT+TGG + chr5.4:4308819-4308838 MS.gene22334:CDS 40.0%
!! TATTTTCTCCACAAGAGCAC+CGG - chr5.4:4307635-4307654 None:intergenic 40.0%
!! TGCCTTCTAAAATAGAGCAG+AGG + chr5.4:4306840-4306859 MS.gene22334:intron 40.0%
!! TGCGGAAGTATTTACACCTT+TGG - chr5.4:4307179-4307198 None:intergenic 40.0%
!! TTGTGCCATTAGTATCGATG+AGG - chr5.4:4308307-4308326 None:intergenic 40.0%
!!! ACAAAATGGGTTGCCGTTTT+GGG + chr5.4:4308335-4308354 MS.gene22334:CDS 40.0%
!!! GCTTGTTTTGGGAAGATTGT+TGG + chr5.4:4307775-4307794 MS.gene22334:CDS 40.0%
!!! TTTTGGGATAAAGGTCACCA+TGG + chr5.4:4308351-4308370 MS.gene22334:CDS 40.0%
AAACTTGGTCCCAGAAATGG+AGG + chr5.4:4309166-4309185 MS.gene22334:CDS 45.0%
AAATCTCTGCGGAAAACTGG+AGG + chr5.4:4307067-4307086 MS.gene22334:CDS 45.0%
AACAGGCAAGAATCCTCAGT+TGG - chr5.4:4308167-4308186 None:intergenic 45.0%
AATACTGGAAGACTCTTGGG+TGG - chr5.4:4306983-4307002 None:intergenic 45.0%
ACAATCTCTGCAATGACTCG+AGG - chr5.4:4306386-4306405 None:intergenic 45.0%
ACACCCTGGCATAAGATTAG+TGG + chr5.4:4308761-4308780 MS.gene22334:CDS 45.0%
ACATTCACCTGATGGAGACA+CGG + chr5.4:4309190-4309209 MS.gene22334:CDS 45.0%
AGGATTCTTGCCTGTTTCCA+TGG + chr5.4:4308171-4308190 MS.gene22334:CDS 45.0%
ATACTGGAAGACTCTTGGGT+GGG - chr5.4:4306982-4307001 None:intergenic 45.0%
ATGACTCGAGGTTGCCTTAA+AGG - chr5.4:4306374-4306393 None:intergenic 45.0%
ATGGAGGAACATTCACCTGA+TGG + chr5.4:4309182-4309201 MS.gene22334:CDS 45.0%
CAGTTAATGATGTAGCAGCC+TGG + chr5.4:4307317-4307336 MS.gene22334:CDS 45.0%
CCAAAACAGATGCTGTTGTG+CGG - chr5.4:4309115-4309134 None:intergenic 45.0%
CCAACATAGCTACCATTCAG+GGG + chr5.4:4307713-4307732 MS.gene22334:CDS 45.0%
CCTAAATCTCTGCGGAAAAC+TGG + chr5.4:4307064-4307083 MS.gene22334:CDS 45.0%
CTACAGGGAACAATTGCCAA+AGG + chr5.4:4307160-4307179 MS.gene22334:CDS 45.0%
CTCACCTTCATGGCATTGTT+GGG - chr5.4:4307476-4307495 None:intergenic 45.0%
CTCCATAAGCAAGAGCTTCA+CGG - chr5.4:4308728-4308747 None:intergenic 45.0%
CTCGCTTCTTTCAAGAGCAA+AGG - chr5.4:4309045-4309064 None:intergenic 45.0%
CTTGTTCTTGCAGTCACAAG+GGG + chr5.4:4306329-4306348 MS.gene22334:CDS 45.0%
GACCAACATAGCTACCATTC+AGG + chr5.4:4307711-4307730 MS.gene22334:CDS 45.0%
GAGGTCCTCATCGATACTAA+TGG + chr5.4:4308299-4308318 MS.gene22334:CDS 45.0%
GCAGAAATTAATCACGCGGA+TGG - chr5.4:4308982-4309001 None:intergenic 45.0%
GCTTATTCCGGCAATAGCAA+TGG - chr5.4:4307104-4307123 None:intergenic 45.0%
GGTGAATGTTCCTCCATTTC+TGG - chr5.4:4309179-4309198 None:intergenic 45.0%
TCTTGCTTATGGAGCTCGTA+TGG + chr5.4:4308734-4308753 MS.gene22334:CDS 45.0%
TGCTACATTAGCTGCTGTTC+TGG + chr5.4:4307519-4307538 MS.gene22334:CDS 45.0%
TTGCCTGTTTCCATGGTTCA+AGG + chr5.4:4308178-4308197 MS.gene22334:CDS 45.0%
! ATTTTTGTTGATCGCGGGCT+TGG + chr5.4:4308630-4308649 MS.gene22334:CDS 45.0%
! CAATGGGGTGCCAATAAACT+TGG + chr5.4:4309151-4309170 MS.gene22334:CDS 45.0%
! CCAGTTTTCCGCAGAGATTT+AGG - chr5.4:4307067-4307086 None:intergenic 45.0%
! GCCAAAATACGGGCTAATAC+AGG - chr5.4:4307244-4307263 None:intergenic 45.0%
! GTCTTGTTCTTGCAGTCACA+AGG + chr5.4:4306327-4306346 MS.gene22334:CDS 45.0%
! GTTGTGCTTTTCTTTGGTGG+TGG + chr5.4:4308696-4308715 MS.gene22334:CDS 45.0%
!! AGCAAGAGCAAGAAGTATCC+AGG - chr5.4:4307338-4307357 None:intergenic 45.0%
!! CACACACGTGGCTTAGTTTT+TGG - chr5.4:4308413-4308432 None:intergenic 45.0%
!! GCTTATTGATGATGACAGGG+AGG + chr5.4:4306899-4306918 MS.gene22334:intron 45.0%
!!! GACAAAATGGGTTGCCGTTT+TGG + chr5.4:4308334-4308353 MS.gene22334:CDS 45.0%
AAAATGCAGACGGCCAACTG+AGG + chr5.4:4308151-4308170 MS.gene22334:CDS 50.0%
ACAATGCCATGAAGGTGAGC+CGG + chr5.4:4307477-4307496 MS.gene22334:CDS 50.0%
ATGGCCCAACAATGCCATGA+AGG + chr5.4:4307469-4307488 MS.gene22334:CDS 50.0%
ATTTCTTGTGGGTCGAAGGC+CGG + chr5.4:4309007-4309026 MS.gene22334:CDS 50.0%
CACAAGAGGAGTGGTCATGA+AGG - chr5.4:4308070-4308089 None:intergenic 50.0%
CCATGTTATTGTGGCGTTCG+AGG + chr5.4:4308383-4308402 MS.gene22334:CDS 50.0%
CCCCTGAATGGTAGCTATGT+TGG - chr5.4:4307716-4307735 None:intergenic 50.0%
CCTCGAACGCCACAATAACA+TGG - chr5.4:4308386-4308405 None:intergenic 50.0%
GAAGATTCCGTGTCTCCATC+AGG - chr5.4:4309200-4309219 None:intergenic 50.0%
GACAGGGAGGAATCCTACTT+GGG + chr5.4:4306912-4306931 MS.gene22334:intron 50.0%
GACCGTGAAGCTCTTGCTTA+TGG + chr5.4:4308723-4308742 MS.gene22334:CDS 50.0%
GCTCACCTTCATGGCATTGT+TGG - chr5.4:4307477-4307496 None:intergenic 50.0%
GGGTTTGTCACGGATGCTAT+CGG + chr5.4:4307547-4307566 MS.gene22334:CDS 50.0%
TCATTGGTCGAACACACACG+TGG - chr5.4:4308425-4308444 None:intergenic 50.0%
TCTTGAAAGAAGCGAGTGCG+CGG + chr5.4:4309049-4309068 MS.gene22334:CDS 50.0%
TGACAGGGAGGAATCCTACT+TGG + chr5.4:4306911-4306930 MS.gene22334:intron 50.0%
TGCAGCTAACACAAGAGGAG+TGG - chr5.4:4308079-4308098 None:intergenic 50.0%
TGGGTCTTCTTAAGTGGGTG+TGG + chr5.4:4307400-4307419 MS.gene22334:CDS 50.0%
TTCCGGCAATAGCAATGGCA+AGG - chr5.4:4307099-4307118 None:intergenic 50.0%
! CCAAAAGATGGAGCGTTTGC+CGG + chr5.4:4307613-4307632 MS.gene22334:CDS 50.0%
! CCGCACAACAGCATCTGTTT+TGG + chr5.4:4309112-4309131 MS.gene22334:CDS 50.0%
! GTCCTTGCCATTGCTATTGC+CGG + chr5.4:4307094-4307113 MS.gene22334:CDS 50.0%
!! AACCACTAGTGTCGGCAGAA+TGG + chr5.4:4307279-4307298 MS.gene22334:CDS 50.0%
!! GCTCTTGCTGTTGCCTTATC+AGG + chr5.4:4307349-4307368 MS.gene22334:CDS 50.0%
!! TTATTCCGTCCAAGCGCTGA+CGG - chr5.4:4306938-4306957 None:intergenic 50.0%
!!! GGGTTGCCGTTTTGGGATAA+AGG + chr5.4:4308342-4308361 MS.gene22334:CDS 50.0%
AGAAGCGAGTGCGCGGAATT+AGG + chr5.4:4309056-4309075 MS.gene22334:CDS 55.0%
AGCCATTCTGCCGACACTAG+TGG - chr5.4:4307284-4307303 None:intergenic 55.0%
ATTCAGGGGCTGCAGTCATG+GGG + chr5.4:4307727-4307746 MS.gene22334:CDS 55.0%
CATTCAGGGGCTGCAGTCAT+GGG + chr5.4:4307726-4307745 MS.gene22334:CDS 55.0%
CCATTAGACGCAGGCTGCAT+CGG - chr5.4:4306248-4306267 None:intergenic 55.0%
CGGGCAAACATTTCGAGCTG+TGG - chr5.4:4306222-4306241 None:intergenic 55.0%
GAGCATGCACCATGCTCAGT+TGG + chr5.4:4308606-4308625 MS.gene22334:CDS 55.0%
GATGCCAACAAGCCACCAAC+AGG - chr5.4:4309083-4309102 None:intergenic 55.0%
GCAAAGGCCAATTCAGCGTC+CGG - chr5.4:4309029-4309048 None:intergenic 55.0%
GCGGAATTAGGACCTGTTGG+TGG + chr5.4:4309068-4309087 MS.gene22334:CDS 55.0%
TCTGGCTGCTGGGTTTGTCA+CGG + chr5.4:4307537-4307556 MS.gene22334:CDS 55.0%
TCTTGCAGTCACAAGGGGAC+TGG + chr5.4:4306334-4306353 MS.gene22334:CDS 55.0%
TGCGCGGAATTAGGACCTGT+TGG + chr5.4:4309065-4309084 MS.gene22334:CDS 55.0%
TTTGTTGATCGCGGGCTTGG+TGG + chr5.4:4308633-4308652 MS.gene22334:CDS 55.0%
! CATCAACCGGCTCACCTTCA+TGG - chr5.4:4307486-4307505 None:intergenic 55.0%
! CCGGCAAACGCTCCATCTTT+TGG - chr5.4:4307616-4307635 None:intergenic 55.0%
! TAGCTGCTGTTCTGGCTGCT+GGG + chr5.4:4307527-4307546 MS.gene22334:CDS 55.0%
! TTAGCTGCTGTTCTGGCTGC+TGG + chr5.4:4307526-4307545 MS.gene22334:CDS 55.0%
!! AGGACCTGTTGGTGGCTTGT+TGG + chr5.4:4309076-4309095 MS.gene22334:CDS 55.0%
AGCGTTTGCCGGTGCTCTTG+TGG + chr5.4:4307624-4307643 MS.gene22334:CDS 60.0%
CCATGACTGCAGCCCCTGAA+TGG - chr5.4:4307728-4307747 None:intergenic 60.0%
CCATTCAGGGGCTGCAGTCA+TGG + chr5.4:4307725-4307744 MS.gene22334:CDS 60.0%
CCGATGCAGCCTGCGTCTAA+TGG + chr5.4:4306245-4306264 MS.gene22334:CDS 60.0%
TCGAAGGCCGGACGCTGAAT+TGG + chr5.4:4309019-4309038 MS.gene22334:CDS 60.0%
TTAGACGCAGGCTGCATCGG+CGG - chr5.4:4306245-4306264 None:intergenic 60.0%
CTACTTGGGCCGTCAGCGCT+TGG + chr5.4:4306926-4306945 MS.gene22334:CDS 65.0%
GCTCGTATGGCCGAACACCC+TGG + chr5.4:4308747-4308766 MS.gene22334:CDS 65.0%
TTGGGCCGTCAGCGCTTGGA+CGG + chr5.4:4306930-4306949 MS.gene22334:CDS 65.0%
!! CAAGCGCTGACGGCCCAAGT+AGG - chr5.4:4306928-4306947 None:intergenic 65.0%
! ACGCAGGCTGCATCGGCGGC+GGG - chr5.4:4306241-4306260 None:intergenic 75.0%
! GACGCAGGCTGCATCGGCGG+CGG - chr5.4:4306242-4306261 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 4306206 4309223 4306206 ID=MS.gene22334
chr5.4 mRNA 4306206 4309223 4306206 ID=MS.gene22334.t1;Parent=MS.gene22334
chr5.4 exon 4306206 4306406 4306206 ID=MS.gene22334.t1.exon1;Parent=MS.gene22334.t1
chr5.4 CDS 4306206 4306406 4306206 ID=cds.MS.gene22334.t1;Parent=MS.gene22334.t1
chr5.4 exon 4306917 4307918 4306917 ID=MS.gene22334.t1.exon2;Parent=MS.gene22334.t1
chr5.4 CDS 4306917 4307918 4306917 ID=cds.MS.gene22334.t1;Parent=MS.gene22334.t1
chr5.4 exon 4308015 4309223 4308015 ID=MS.gene22334.t1.exon3;Parent=MS.gene22334.t1
chr5.4 CDS 4308015 4309223 4308015 ID=cds.MS.gene22334.t1;Parent=MS.gene22334.t1
Gene Sequence

>MS.gene22334

ATGACTTCCAATACCACAGCTCGAAATGTTTGCCCGCCGCCGATGCAGCCTGCGTCTAATGGTGTATTTCAAGGAGATCACCCTCTTGATTATGCACTTCCATTGGCTATTTTACAGATATGTCTTGTTCTTGCAGTCACAAGGGGACTGGCCTATCTTCTAAAACCTTTAAGGCAACCTCGAGTCATTGCAGAGATTGTTGTAAGTTCTTTTATTTTTGTTTAAGTATTTACTTAATTTAATGATTTTTTTTTGTTAACTGATATTAATAATTGATATTAGTAAAGAATTTTATATATTAGAAACTAGTGGGGAAATTGGCAAACAATGCAACAATAAGAGCCTTGAGTGTTCCATTTTTATATAATTTCACAGTTTTGAGTTTCAACTACGTTCTACTTGTCCAAACTATGAAACAAGTTCTAATATTTAGTCAATTTTTTATGTAGAGATTAGAATGCAATATGACTATAAAAAATTCACTGTTCTTAAATCAACAATAGATGTTAGAGAAATAGACATGCCAAAATGTGCACATGTTTAAGAGAAATTAAAATTGAATCATATTAAAATCGCAGAGAGTTTAAACACACTTGGAACTTCATTAAATCCACATTTGGTCAGTCTTACTAATTGCCTTCTAAAATAGAGCAGAGGTTTTTTCAACTTCTATTTTTGTGTGTTAATTAGATTGCTTATTGATGATGACAGGGAGGAATCCTACTTGGGCCGTCAGCGCTTGGACGGAATAAAAGCTATCTACATGCAGTTTTCCCACCCAAGAGTCTTCCAGTATTAGATACTCTAGCAAACCTTGGTCTTATATTCTTTCTATTTTTAGCAGGCATAGAGTTAGATCCTAAATCTCTGCGGAAAACTGGAGGTAGAGTCCTTGCCATTGCTATTGCCGGAATAAGCTTACCCTTTGCACTAGGAATTGGTTCATCGTTTGTTCTACAGGGAACAATTGCCAAAGGTGTAAATACTTCCGCATTTCTTGTATATATGGGGGTTGCTCTGTCTATAACTGCTTTTCCTGTATTAGCCCGTATTTTGGCCGAGTTAAAACTTCTAACCACTAGTGTCGGCAGAATGGCTATGTCAGCTGCAGCAGTTAATGATGTAGCAGCCTGGATACTTCTTGCTCTTGCTGTTGCCTTATCAGGCAATAGCCAATCTCCATTTGTGTCATTGTGGGTCTTCTTAAGTGGGTGTGGTTTTGTTGTATGTTCAATCCTCATTGTCCTTCCAATTTTCAAATGGATGGCCCAACAATGCCATGAAGGTGAGCCGGTTGATGAGTTATACATATGTGCTACATTAGCTGCTGTTCTGGCTGCTGGGTTTGTCACGGATGCTATCGGAATTCATGCCATGTTTGGCGCGTTTGTTTTTGGAATTTTGGTTCCAAAAGATGGAGCGTTTGCCGGTGCTCTTGTGGAGAAAATAGAGGATCTTGTATCTGGTCTATTACTCCCACTTTATTTTGTGTCAAGTGGATTGAAGACCAACATAGCTACCATTCAGGGGCTGCAGTCATGGGGTCTTCTAGTTTTCGTTACATTTACGGCTTGTTTTGGGAAGATTGTTGGGACTATTGTAGTGTCTCTTCTATGTAAAGTACCTTTGAACGAGTCTTTAGTGCTTGGATTTTTGATGAATAGTAAAGGCTTGGTTGAATTAATTGTCCTCAACATCGGTAAAGATAGAAAGGTATTCGAATTTATTTTCTTGCTCCTCATTATTCCTATAATTAATTTAAAATGGTGACCGATCATCGTGATGTATATTTGATTTACGTATCTGCAGGTTTTGAATGATCAGACCTTTGCTATTATGGTTTTCATGGCTCTCATTACTACCTTCATGACCACTCCTCTTGTGTTAGCTGCATATAAGAGGAAAGAAAGAAAATCTAATTACAAATACAGAACAATTGAAAGGAAAAATGCAGACGGCCAACTGAGGATTCTTGCCTGTTTCCATGGTTCAAGGAATATTCCATCGATGATAAACTTGATTGAGGCTTCAAGAGGAATCAAGAAGCATGACACACTTTGTGTCTATGCAATGCACCTTAAAGAATTTTGTGAGAGGTCCTCATCGATACTAATGGCACAAAAGGTAAGACAAAATGGGTTGCCGTTTTGGGATAAAGGTCACCATGGAGATTCTGTCCATGTTATTGTGGCGTTCGAGGCTTACCAAAAACTAAGCCACGTGTGTGTTCGACCAATGATAGCAATTTCATCAATGGCGAATATTCATGAAGACATTTGTGCAACTGCTGATAGAAAGAGAGCCGCTGTCATCATTCTTCCATTTCATAAGCAACAAAGGTTAGACGGTTCACTAGACATTATAAGAAACGATTTTCGATTGGTTAACAAAAGGGTACTCGAGCATGCACCATGCTCAGTTGGAATTTTTGTTGATCGCGGGCTTGGTGGTTCATGTCATGTGTCTGCAAGTAATGTTTCCTATTGCATTGTTGTGCTTTTCTTTGGTGGTGGTGATGACCGTGAAGCTCTTGCTTATGGAGCTCGTATGGCCGAACACCCTGGCATAAGATTAGTGGTTTTTCGCTTCTTATTGGAACCTAATATTGTAGGACAGATCACCAAAGTTGATGTTGGAGACTCTTCTAGCAGTAATTCAGTATCAGATTCTGAAGATGACGAGTTTCTTGCTGAATTTAAGCTAAAAACAGCCAGTGATGACTCAATTATCTATGAAGAAAAAATAGTAAAAGATGCTGCAGAAACAGTTGCTACCATCCGCGTGATTAATTTCTGCAACTTATTTCTTGTGGGTCGAAGGCCGGACGCTGAATTGGCCTTTGCTCTTGAAAGAAGCGAGTGCGCGGAATTAGGACCTGTTGGTGGCTTGTTGGCATCACAAGATTTCCGCACAACAGCATCTGTTTTGGTGATGCAACAGTATCACAATGGGGTGCCAATAAACTTGGTCCCAGAAATGGAGGAACATTCACCTGATGGAGACACGGAATCTTCTTAA
Protein sequence

>MS.gene22334.t1

MTSNTTARNVCPPPMQPASNGVFQGDHPLDYALPLAILQICLVLAVTRGLAYLLKPLRQPRVIAEIVGGILLGPSALGRNKSYLHAVFPPKSLPVLDTLANLGLIFFLFLAGIELDPKSLRKTGGRVLAIAIAGISLPFALGIGSSFVLQGTIAKGVNTSAFLVYMGVALSITAFPVLARILAELKLLTTSVGRMAMSAAAVNDVAAWILLALAVALSGNSQSPFVSLWVFLSGCGFVVCSILIVLPIFKWMAQQCHEGEPVDELYICATLAAVLAAGFVTDAIGIHAMFGAFVFGILVPKDGAFAGALVEKIEDLVSGLLLPLYFVSSGLKTNIATIQGLQSWGLLVFVTFTACFGKIVGTIVVSLLCKVPLNESLVLGFLMNSKGLVELIVLNIGKDRKVLNDQTFAIMVFMALITTFMTTPLVLAAYKRKERKSNYKYRTIERKNADGQLRILACFHGSRNIPSMINLIEASRGIKKHDTLCVYAMHLKEFCERSSSILMAQKVRQNGLPFWDKGHHGDSVHVIVAFEAYQKLSHVCVRPMIAISSMANIHEDICATADRKRAAVIILPFHKQQRLDGSLDIIRNDFRLVNKRVLEHAPCSVGIFVDRGLGGSCHVSASNVSYCIVVLFFGGGDDREALAYGARMAEHPGIRLVVFRFLLEPNIVGQITKVDVGDSSSSNSVSDSEDDEFLAEFKLKTASDDSIIYEEKIVKDAAETVATIRVINFCNLFLVGRRPDAELAFALERSECAELGPVGGLLASQDFRTTASVLVMQQYHNGVPINLVPEMEEHSPDGDTESS