AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22541

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22541.t1 AT5G12010 58.837 430 151 4 1 417 86 502 0.0 528
MS.gene22541.t1 AT4G29780 59.620 421 164 4 1 417 122 540 5.56e-177 505
MS.gene22541.t1 AT3G55350 30.368 326 188 9 76 374 53 366 1.76e-43 157
MS.gene22541.t1 AT3G63270 29.618 314 188 10 76 363 43 349 8.39e-39 144
MS.gene22541.t1 AT3G19120 27.362 307 191 9 88 376 114 406 3.42e-24 104
MS.gene22541.t1 AT1G72275 26.797 306 162 14 88 374 92 354 5.08e-19 89.0
MS.gene22541.t1 AT1G72275 26.797 306 162 14 88 374 92 354 5.53e-19 89.0
MS.gene22541.t1 AT5G41980 22.222 306 215 6 87 389 38 323 2.43e-11 65.5

Find 96 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTTCAAATTTCCTAATT+TGG 0.108987 2.4:+49826943 None:intergenic
TAGGAAATTTGAAGAATATT+TGG 0.147062 2.4:-49826937 MS.gene22541:CDS
TACATTACACCACCAATATT+TGG 0.169757 2.4:+49827175 None:intergenic
CAAGATTTGCCTGTTGTTCT+TGG 0.174992 2.4:-49826728 MS.gene22541:CDS
AAATTCAGGTTATTCACTTA+TGG 0.234627 2.4:-49826906 MS.gene22541:CDS
ATTAGTGAAATTGAGAAAAT+TGG 0.245237 2.4:-49826818 MS.gene22541:CDS
GCTCATTATTTGTTACATTA+TGG 0.252231 2.4:-49826563 MS.gene22541:CDS
ATTTGGATAGTTGGAAATTC+AGG 0.257969 2.4:-49826920 MS.gene22541:CDS
GTCAGATTCTTATGCTTATA+TGG 0.273058 2.4:+49826869 None:intergenic
TCCTTCATTGTTTCTTCATC+TGG 0.276668 2.4:+49827232 None:intergenic
TTGAAGAATATTTGGATAGT+TGG 0.287680 2.4:-49826929 MS.gene22541:CDS
TCAGCTATTGTATCTGGTTC+TGG 0.289548 2.4:-49827667 MS.gene22541:CDS
AATGCAAGATAATGCAGTTT+TGG 0.294783 2.4:-49826990 MS.gene22541:CDS
TTCTTCCATGTAATGATTCA+TGG 0.295521 2.4:+49827771 None:intergenic
GCTGCTTCAGCTATTGTATC+TGG 0.300058 2.4:-49827673 MS.gene22541:CDS
TTATATGAGAGGGCCAAATT+AGG 0.319401 2.4:-49826956 MS.gene22541:CDS
TTTACCTTAGGTGCAATTAT+AGG 0.323232 2.4:+49827139 None:intergenic
AGTTTCGGTATTCCAAATAT+TGG 0.326727 2.4:-49827187 MS.gene22541:CDS
CAACAAGCACCAAGAACAAC+AGG 0.334974 2.4:+49826719 None:intergenic
AGGCTGGTTTCGAAGAAATT+CGG 0.335907 2.4:-49827340 MS.gene22541:CDS
ACCGCCACACGCTGGCGAAC+CGG 0.336332 2.4:+49827397 None:intergenic
TTGTTCATTGGATTCTTTGA+AGG 0.351985 2.4:-49826600 MS.gene22541:CDS
GGCTGGTTTCGAAGAAATTC+GGG 0.358679 2.4:-49827339 MS.gene22541:CDS
AGGTTATTCACTTATGGATT+GGG 0.375307 2.4:-49826900 MS.gene22541:CDS
CAGGTTATTCACTTATGGAT+TGG 0.377146 2.4:-49826901 MS.gene22541:CDS
GAAGAGTTTGAGGGCAGTTT+CGG 0.380290 2.4:-49827202 MS.gene22541:CDS
CATTATGGTCGGGCTGGTAC+TGG 0.384233 2.4:-49826548 MS.gene22541:CDS
GACGCTGCGTGATGCTATTC+CGG 0.397950 2.4:-49827416 MS.gene22541:CDS
TGAGGATGGTGCTTGTTCAT+TGG 0.398134 2.4:-49826612 MS.gene22541:CDS
TCGCCACTGGCGAACCGCTT+AGG 0.411688 2.4:-49827360 MS.gene22541:CDS
TTGCAACCGTGAAGATTTCC+CGG 0.413793 2.4:-49827530 MS.gene22541:CDS
CAACTCTCCCACCAGTCTTT+AGG 0.414933 2.4:+49827550 None:intergenic
TGAGATTATGAATGAAGAAA+TGG 0.419917 2.4:-49826678 MS.gene22541:CDS
ACCCACAGCCTACGTTGAAT+AGG 0.424840 2.4:+49827583 None:intergenic
AGCTATTCAGTAACTGTTCA+AGG 0.427841 2.4:-49827067 MS.gene22541:CDS
ATTTCCCGGATGATGAATTC+CGG 0.433406 2.4:-49827516 MS.gene22541:CDS
CACTGGCGAACCGCTTAGGC+TGG 0.434157 2.4:-49827356 MS.gene22541:CDS
TGGTCCAGACCAACGTGGTC+TGG 0.437477 2.4:-49827647 MS.gene22541:CDS
TTGTTGTTGTTTGATGATGT+TGG 0.444958 2.4:+49827722 None:intergenic
GTTTCGAAGAAATTCGGGTT+AGG 0.455239 2.4:-49827334 MS.gene22541:CDS
ATTATTTGTTACATTATGGT+CGG 0.457532 2.4:-49826559 MS.gene22541:CDS
TGGTCCAGACCACGTTGGTC+TGG 0.459547 2.4:+49827643 None:intergenic
AGCCTATTCAACGTAGGCTG+TGG 0.468864 2.4:-49827585 MS.gene22541:CDS
GATGTGTGTATTGGTTGGCC+TGG 0.470677 2.4:-49827016 MS.gene22541:CDS
CATCGCCGGAATTCATCATC+CGG 0.483016 2.4:+49827511 None:intergenic
TTATTTGTTACATTATGGTC+GGG 0.485943 2.4:-49826558 MS.gene22541:CDS
TTACTGATGTGTGTATTGGT+TGG 0.498611 2.4:-49827021 MS.gene22541:CDS
TACAAACCAACATTTACCTT+AGG 0.499068 2.4:+49827127 None:intergenic
TTCGGTATTCCAAATATTGG+TGG 0.501746 2.4:-49827184 MS.gene22541:CDS
TAGTAGGTTGAAAGGTAGGT+GGG 0.506976 2.4:-49826783 MS.gene22541:CDS
CATCATCCGGGAAATCTTCA+CGG 0.507925 2.4:+49827524 None:intergenic
CAAGGTGTGGTAAATAGTAA+AGG 0.508102 2.4:-49827049 MS.gene22541:CDS
TGAAAGACCGGCCTAAAGAC+TGG 0.509719 2.4:-49827561 MS.gene22541:CDS
ATGAAGAAATGGATGATGAA+TGG 0.517962 2.4:-49826667 MS.gene22541:CDS
GTAGGCTGTGGGTGAAAGAC+CGG 0.517962 2.4:-49827573 MS.gene22541:CDS
GCATTATCTTGCATTGAGCC+AGG 0.518982 2.4:+49826998 None:intergenic
CTGCAACGAGCTGGATTCAT+CGG 0.520703 2.4:-49827455 MS.gene22541:CDS
TGTTCCTATAATTGCACCTA+AGG 0.522840 2.4:-49827143 MS.gene22541:CDS
ATTGCACCTAAGGTAAATGT+TGG 0.524057 2.4:-49827133 MS.gene22541:CDS
TGTATATACCGCCTCGCCAC+TGG 0.524677 2.4:-49827373 MS.gene22541:CDS
CAACATGATCTGCAACGAGC+TGG 0.528812 2.4:-49827464 MS.gene22541:CDS
TTGTTACATTATGGTCGGGC+TGG 0.531532 2.4:-49826554 MS.gene22541:CDS
GGAAACGAAAGAAGAGTTTG+AGG 0.534530 2.4:-49827212 MS.gene22541:CDS
TCCGGTTCGCCAGCGTGTGG+CGG 0.537854 2.4:-49827398 MS.gene22541:CDS
TAATGCAGAAGTTTCTTCGT+TGG 0.539378 2.4:-49827255 MS.gene22541:CDS
GGATGATGAATGGAAGTTTG+AGG 0.540969 2.4:-49826657 MS.gene22541:CDS
GCCTATTCAACGTAGGCTGT+GGG 0.545772 2.4:-49827584 MS.gene22541:CDS
GCCTACGTTGAATAGGCTGC+TGG 0.548098 2.4:+49827590 None:intergenic
TAAGCGGTTCGCCAGTGGCG+AGG 0.549583 2.4:+49827362 None:intergenic
GCCAGATGAAGAAACAATGA+AGG 0.551617 2.4:-49827233 MS.gene22541:CDS
GAAACGAAAGAAGAGTTTGA+GGG 0.564474 2.4:-49827211 MS.gene22541:CDS
ATCGCCGGAATTCATCATCC+GGG 0.580423 2.4:+49827512 None:intergenic
CAGCCTAAGCGGTTCGCCAG+TGG 0.580669 2.4:+49827357 None:intergenic
TACTCATCCGAAAACATCGC+CGG 0.582531 2.4:+49827497 None:intergenic
TGATGATGAAATGGTTGTTG+AGG 0.584227 2.4:-49826630 MS.gene22541:CDS
TTCTTCGAAACCAGCCTAAG+CGG 0.591810 2.4:+49827346 None:intergenic
TTAGTAGGTTGAAAGGTAGG+TGG 0.594060 2.4:-49826784 MS.gene22541:CDS
GTAGGTTGAAAGGTAGGTGG+GGG 0.595193 2.4:-49826781 MS.gene22541:CDS
GATGAAATGGTTGTTGAGGA+TGG 0.597572 2.4:-49826626 MS.gene22541:CDS
TTCAGTAACTGTTCAAGGTG+TGG 0.599865 2.4:-49827062 MS.gene22541:CDS
AGTAGGTTGAAAGGTAGGTG+GGG 0.601658 2.4:-49826782 MS.gene22541:CDS
ACAAACCATGAATCATTACA+TGG 0.604340 2.4:-49827776 None:intergenic
GCGGTTCGCCAGTGGCGAGG+CGG 0.606304 2.4:+49827365 None:intergenic
CAACGTGGTCTGGACCAGCC+CGG 0.612903 2.4:-49827637 MS.gene22541:CDS
AGACCGGCCTAAAGACTGGT+GGG 0.625890 2.4:-49827557 MS.gene22541:CDS
TCTCCCACCAGTCTTTAGGC+CGG 0.626133 2.4:+49827554 None:intergenic
GTTGGTCTAACTGAGAGTAA+TGG 0.636217 2.4:+49827740 None:intergenic
AATCTGACGTGGACACAACA+TGG 0.637422 2.4:-49826854 MS.gene22541:CDS
TATTCCGGTTCGCCAGCGTG+TGG 0.653116 2.4:-49827401 MS.gene22541:CDS
AAGACCGGCCTAAAGACTGG+TGG 0.667437 2.4:-49827558 MS.gene22541:CDS
TAACACAAGCTTATGACACG+TGG 0.669447 2.4:+49827306 None:intergenic
CGGGCTGGTCCAGACCACGT+TGG 0.734063 2.4:+49827638 None:intergenic
GGTTCTGGTCCAGACCAACG+TGG 0.734063 2.4:-49827652 MS.gene22541:CDS
ACCAGCAGCCTATTCAACGT+AGG 0.741133 2.4:-49827591 MS.gene22541:CDS
ATATACATACCGCCACACGC+TGG 0.742881 2.4:+49827389 None:intergenic
ATAAGCATAAGAATCTGACG+TGG 0.747957 2.4:-49826865 MS.gene22541:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGTGAAATTGAGAAAAT+TGG - chr2.4:49827489-49827508 MS.gene22541:CDS 20.0%
!! ATTCTTCAAATTTCCTAATT+TGG + chr2.4:49827367-49827386 None:intergenic 20.0%
!! TAGGAAATTTGAAGAATATT+TGG - chr2.4:49827370-49827389 MS.gene22541:CDS 20.0%
!!! ATTATTTGTTACATTATGGT+CGG - chr2.4:49827748-49827767 MS.gene22541:CDS 20.0%
! TGAGATTATGAATGAAGAAA+TGG - chr2.4:49827629-49827648 MS.gene22541:CDS 25.0%
! TTGAAGAATATTTGGATAGT+TGG - chr2.4:49827378-49827397 MS.gene22541:CDS 25.0%
!! AAATTCAGGTTATTCACTTA+TGG - chr2.4:49827401-49827420 MS.gene22541:CDS 25.0%
!! AATATCTTTTGAATCTGTAC+CGG + chr2.4:49826692-49826711 None:intergenic 25.0%
!! GCTCATTATTTGTTACATTA+TGG - chr2.4:49827744-49827763 MS.gene22541:CDS 25.0%
!! TTATTTGTTACATTATGGTC+GGG - chr2.4:49827749-49827768 MS.gene22541:CDS 25.0%
!!! AAAAAAGTGCTTTATATGAG+AGG - chr2.4:49827340-49827359 MS.gene22541:CDS 25.0%
!!! AAAAAGTGCTTTATATGAGA+GGG - chr2.4:49827341-49827360 MS.gene22541:CDS 25.0%
!!! TTACTGAATAGCTAGTTTTT+TGG + chr2.4:49827233-49827252 None:intergenic 25.0%
AGGTTATTCACTTATGGATT+GGG - chr2.4:49827407-49827426 MS.gene22541:CDS 30.0%
AGTTTCGGTATTCCAAATAT+TGG - chr2.4:49827120-49827139 MS.gene22541:CDS 30.0%
ATGAAGAAATGGATGATGAA+TGG - chr2.4:49827640-49827659 MS.gene22541:CDS 30.0%
ATTTGGATAGTTGGAAATTC+AGG - chr2.4:49827387-49827406 MS.gene22541:CDS 30.0%
GTCAGATTCTTATGCTTATA+TGG + chr2.4:49827441-49827460 None:intergenic 30.0%
TACAAACCAACATTTACCTT+AGG + chr2.4:49827183-49827202 None:intergenic 30.0%
TACATTACACCACCAATATT+TGG + chr2.4:49827135-49827154 None:intergenic 30.0%
TTAACAAAAAACTCACTGAG+AGG - chr2.4:49827205-49827224 MS.gene22541:CDS 30.0%
TTGTTCATTGGATTCTTTGA+AGG - chr2.4:49827707-49827726 MS.gene22541:CDS 30.0%
TTTACCTTAGGTGCAATTAT+AGG + chr2.4:49827171-49827190 None:intergenic 30.0%
! AATGCAAGATAATGCAGTTT+TGG - chr2.4:49827317-49827336 MS.gene22541:CDS 30.0%
! ATATCTTTTGAATCTGTACC+GGG + chr2.4:49826691-49826710 None:intergenic 30.0%
! GTTTTTACTGATGTGTGTAT+TGG - chr2.4:49827282-49827301 MS.gene22541:CDS 30.0%
! TTGGTAAAGATGCTTTTAGT+AGG - chr2.4:49827508-49827527 MS.gene22541:CDS 30.0%
!! TTGTTGTTGTTTGATGATGT+TGG + chr2.4:49826588-49826607 None:intergenic 30.0%
!!! TGAGGTTTTTGATGATGAAA+TGG - chr2.4:49827668-49827687 MS.gene22541:CDS 30.0%
AGCTATTCAGTAACTGTTCA+AGG - chr2.4:49827240-49827259 MS.gene22541:CDS 35.0%
ATAAGCATAAGAATCTGACG+TGG - chr2.4:49827442-49827461 MS.gene22541:CDS 35.0%
ATTGCACCTAAGGTAAATGT+TGG - chr2.4:49827174-49827193 MS.gene22541:CDS 35.0%
CAAGGTGTGGTAAATAGTAA+AGG - chr2.4:49827258-49827277 MS.gene22541:CDS 35.0%
CAGGTTATTCACTTATGGAT+TGG - chr2.4:49827406-49827425 MS.gene22541:CDS 35.0%
GAAACGAAAGAAGAGTTTGA+GGG - chr2.4:49827096-49827115 MS.gene22541:CDS 35.0%
TAATGCAGAAGTTTCTTCGT+TGG - chr2.4:49827052-49827071 MS.gene22541:CDS 35.0%
TGTTCCTATAATTGCACCTA+AGG - chr2.4:49827164-49827183 MS.gene22541:CDS 35.0%
TTATATGAGAGGGCCAAATT+AGG - chr2.4:49827351-49827370 MS.gene22541:CDS 35.0%
TTCGGTATTCCAAATATTGG+TGG - chr2.4:49827123-49827142 MS.gene22541:CDS 35.0%
! TCCTTCATTGTTTCTTCATC+TGG + chr2.4:49827078-49827097 None:intergenic 35.0%
! TGATGATGAAATGGTTGTTG+AGG - chr2.4:49827677-49827696 MS.gene22541:CDS 35.0%
! TTACTGATGTGTGTATTGGT+TGG - chr2.4:49827286-49827305 MS.gene22541:CDS 35.0%
!! CTTTTAGTAGGTTGAAAGGT+AGG - chr2.4:49827520-49827539 MS.gene22541:CDS 35.0%
!! GATGCTTTTAGTAGGTTGAA+AGG - chr2.4:49827516-49827535 MS.gene22541:CDS 35.0%
ATTTCCCGGATGATGAATTC+CGG - chr2.4:49826791-49826810 MS.gene22541:CDS 40.0%
CAAGATTTGCCTGTTGTTCT+TGG - chr2.4:49827579-49827598 MS.gene22541:CDS 40.0%
GATGAAATGGTTGTTGAGGA+TGG - chr2.4:49827681-49827700 MS.gene22541:CDS 40.0%
GCCAGATGAAGAAACAATGA+AGG - chr2.4:49827074-49827093 MS.gene22541:CDS 40.0%
GGAAACGAAAGAAGAGTTTG+AGG - chr2.4:49827095-49827114 MS.gene22541:CDS 40.0%
GGATGATGAATGGAAGTTTG+AGG - chr2.4:49827650-49827669 MS.gene22541:CDS 40.0%
TAACACAAGCTTATGACACG+TGG + chr2.4:49827004-49827023 None:intergenic 40.0%
TTCAGTAACTGTTCAAGGTG+TGG - chr2.4:49827245-49827264 MS.gene22541:CDS 40.0%
! ATGAATTCCGGCGATGTTTT+CGG - chr2.4:49826803-49826822 MS.gene22541:CDS 40.0%
! GTTGGTCTAACTGAGAGTAA+TGG + chr2.4:49826570-49826589 None:intergenic 40.0%
! GTTTCGAAGAAATTCGGGTT+AGG - chr2.4:49826973-49826992 MS.gene22541:CDS 40.0%
! TAGTAGGTTGAAAGGTAGGT+GGG - chr2.4:49827524-49827543 MS.gene22541:CDS 40.0%
! TCAGCTATTGTATCTGGTTC+TGG - chr2.4:49826640-49826659 MS.gene22541:CDS 40.0%
! TTAGTAGGTTGAAAGGTAGG+TGG - chr2.4:49827523-49827542 MS.gene22541:CDS 40.0%
!! AGGCTGGTTTCGAAGAAATT+CGG - chr2.4:49826967-49826986 MS.gene22541:CDS 40.0%
AATCTGACGTGGACACAACA+TGG - chr2.4:49827453-49827472 MS.gene22541:CDS 45.0%
CAACAAGCACCAAGAACAAC+AGG + chr2.4:49827591-49827610 None:intergenic 45.0%
CATCATCCGGGAAATCTTCA+CGG + chr2.4:49826786-49826805 None:intergenic 45.0%
GAAGAGTTTGAGGGCAGTTT+CGG - chr2.4:49827105-49827124 MS.gene22541:CDS 45.0%
GCATTATCTTGCATTGAGCC+AGG + chr2.4:49827312-49827331 None:intergenic 45.0%
GCTGCTTCAGCTATTGTATC+TGG - chr2.4:49826634-49826653 MS.gene22541:CDS 45.0%
TACTCATCCGAAAACATCGC+CGG + chr2.4:49826813-49826832 None:intergenic 45.0%
TGAGGATGGTGCTTGTTCAT+TGG - chr2.4:49827695-49827714 MS.gene22541:CDS 45.0%
TTCTTCGAAACCAGCCTAAG+CGG + chr2.4:49826964-49826983 None:intergenic 45.0%
TTGCAACCGTGAAGATTTCC+CGG - chr2.4:49826777-49826796 MS.gene22541:CDS 45.0%
TTGTTACATTATGGTCGGGC+TGG - chr2.4:49827753-49827772 MS.gene22541:CDS 45.0%
! AGTAGGTTGAAAGGTAGGTG+GGG - chr2.4:49827525-49827544 MS.gene22541:CDS 45.0%
!! GGCTGGTTTCGAAGAAATTC+GGG - chr2.4:49826968-49826987 MS.gene22541:CDS 45.0%
ACCAGCAGCCTATTCAACGT+AGG - chr2.4:49826716-49826735 MS.gene22541:CDS 50.0%
ACCCACAGCCTACGTTGAAT+AGG + chr2.4:49826727-49826746 None:intergenic 50.0%
AGCCTATTCAACGTAGGCTG+TGG - chr2.4:49826722-49826741 MS.gene22541:CDS 50.0%
ATATACATACCGCCACACGC+TGG + chr2.4:49826921-49826940 None:intergenic 50.0%
ATCGCCGGAATTCATCATCC+GGG + chr2.4:49826798-49826817 None:intergenic 50.0%
CAACATGATCTGCAACGAGC+TGG - chr2.4:49826843-49826862 MS.gene22541:CDS 50.0%
CAACTCTCCCACCAGTCTTT+AGG + chr2.4:49826760-49826779 None:intergenic 50.0%
CATCGCCGGAATTCATCATC+CGG + chr2.4:49826799-49826818 None:intergenic 50.0%
CTGCAACGAGCTGGATTCAT+CGG - chr2.4:49826852-49826871 MS.gene22541:CDS 50.0%
GCCTATTCAACGTAGGCTGT+GGG - chr2.4:49826723-49826742 MS.gene22541:CDS 50.0%
TGAAAGACCGGCCTAAAGAC+TGG - chr2.4:49826746-49826765 MS.gene22541:CDS 50.0%
! CTTTTGAATCTGTACCGGGC+TGG + chr2.4:49826687-49826706 None:intergenic 50.0%
! GTAGGTTGAAAGGTAGGTGG+GGG - chr2.4:49827526-49827545 MS.gene22541:CDS 50.0%
!! GATGTGTGTATTGGTTGGCC+TGG - chr2.4:49827291-49827310 MS.gene22541:CDS 50.0%
!! GGTGGGGGTGTTTGAAGAAA+AGG - chr2.4:49827541-49827560 MS.gene22541:CDS 50.0%
AAGACCGGCCTAAAGACTGG+TGG - chr2.4:49826749-49826768 MS.gene22541:CDS 55.0%
AGACCGGCCTAAAGACTGGT+GGG - chr2.4:49826750-49826769 MS.gene22541:CDS 55.0%
GACGCTGCGTGATGCTATTC+CGG - chr2.4:49826891-49826910 MS.gene22541:CDS 55.0%
GCCTACGTTGAATAGGCTGC+TGG + chr2.4:49826720-49826739 None:intergenic 55.0%
GTAGGCTGTGGGTGAAAGAC+CGG - chr2.4:49826734-49826753 MS.gene22541:CDS 55.0%
TCTCCCACCAGTCTTTAGGC+CGG + chr2.4:49826756-49826775 None:intergenic 55.0%
TGTATATACCGCCTCGCCAC+TGG - chr2.4:49826934-49826953 MS.gene22541:CDS 55.0%
!! CATTATGGTCGGGCTGGTAC+TGG - chr2.4:49827759-49827778 MS.gene22541:CDS 55.0%
TGGTCCAGACCAACGTGGTC+TGG - chr2.4:49826660-49826679 MS.gene22541:CDS 60.0%
TGGTCCAGACCACGTTGGTC+TGG + chr2.4:49826667-49826686 None:intergenic 60.0%
! GGTTCTGGTCCAGACCAACG+TGG - chr2.4:49826655-49826674 MS.gene22541:CDS 60.0%
! TATTCCGGTTCGCCAGCGTG+TGG - chr2.4:49826906-49826925 MS.gene22541:CDS 60.0%
CAACGTGGTCTGGACCAGCC+CGG - chr2.4:49826670-49826689 MS.gene22541:CDS 65.0%
CACTGGCGAACCGCTTAGGC+TGG - chr2.4:49826951-49826970 MS.gene22541:CDS 65.0%
CAGCCTAAGCGGTTCGCCAG+TGG + chr2.4:49826953-49826972 None:intergenic 65.0%
TAAGCGGTTCGCCAGTGGCG+AGG + chr2.4:49826948-49826967 None:intergenic 65.0%
TCGCCACTGGCGAACCGCTT+AGG - chr2.4:49826947-49826966 MS.gene22541:CDS 65.0%
ACCGCCACACGCTGGCGAAC+CGG + chr2.4:49826913-49826932 None:intergenic 70.0%
! TCCGGTTCGCCAGCGTGTGG+CGG - chr2.4:49826909-49826928 MS.gene22541:CDS 70.0%
!! CGGGCTGGTCCAGACCACGT+TGG + chr2.4:49826672-49826691 None:intergenic 70.0%
GCGGTTCGCCAGTGGCGAGG+CGG + chr2.4:49826945-49826964 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 49826538 49827791 49826538 ID=MS.gene22541
chr2.4 mRNA 49826538 49827791 49826538 ID=MS.gene22541.t1;Parent=MS.gene22541
chr2.4 exon 49826538 49827791 49826538 ID=MS.gene22541.t1.exon1;Parent=MS.gene22541.t1
chr2.4 CDS 49826538 49827791 49826538 ID=cds.MS.gene22541.t1;Parent=MS.gene22541.t1
Gene Sequence

>MS.gene22541

ATGAATCATTACATGGAAGAACTTCAAAACCATTACTCTCAGTTAGACCAACATCATCAAACAACAACAAAAAAAGCTCGTATGTCTGCTATTACAGCTGCTTCAGCTATTGTATCTGGTTCTGGTCCAGACCAACGTGGTCTGGACCAGCCCGGTACAGATTCAAAAGATATTATCAACCAGCAGCCTATTCAACGTAGGCTGTGGGTGAAAGACCGGCCTAAAGACTGGTGGGAGAGTTGCAACCGTGAAGATTTCCCGGATGATGAATTCCGGCGATGTTTTCGGATGAGTAAAAAAACATTCAACATGATCTGCAACGAGCTGGATTCATCGGTAACGAAGAAAAACACGACGCTGCGTGATGCTATTCCGGTTCGCCAGCGTGTGGCGGTATGTATATACCGCCTCGCCACTGGCGAACCGCTTAGGCTGGTTTCGAAGAAATTCGGGTTAGGAATATCCACGTGTCATAAGCTTGTGTTAGAAGTTTGTGCAGCTATTAAAACAGTTTTAATGCAGAAGTTTCTTCGTTGGCCAGATGAAGAAACAATGAAGGAAACGAAAGAAGAGTTTGAGGGCAGTTTCGGTATTCCAAATATTGGTGGTGTAATGTATACTACTCATGTTCCTATAATTGCACCTAAGGTAAATGTTGGTTTGTATTTTAACAAAAAACTCACTGAGAGGAACCAAAAAACTAGCTATTCAGTAACTGTTCAAGGTGTGGTAAATAGTAAAGGTGTTTTTACTGATGTGTGTATTGGTTGGCCTGGCTCAATGCAAGATAATGCAGTTTTGGAAAAAAGTGCTTTATATGAGAGGGCCAAATTAGGAAATTTGAAGAATATTTGGATAGTTGGAAATTCAGGTTATTCACTTATGGATTGGGTTTTAGTGCCATATAAGCATAAGAATCTGACGTGGACACAACATGGTTTTAATGAGAGAATTAGTGAAATTGAGAAAATTGGTAAAGATGCTTTTAGTAGGTTGAAAGGTAGGTGGGGGTGTTTGAAGAAAAGGACTGAAATTAAGTTGCAAGATTTGCCTGTTGTTCTTGGTGCTTGTTGTGTGCTTCATAATATTTGTGAGATTATGAATGAAGAAATGGATGATGAATGGAAGTTTGAGGTTTTTGATGATGAAATGGTTGTTGAGGATGGTGCTTGTTCATTGGATTCTTTGAAGGCTAGAGATCAAATTGCTCATTATTTGTTACATTATGGTCGGGCTGGTACTGGATTTTTATAG
Protein sequence

>MS.gene22541.t1

MNHYMEELQNHYSQLDQHHQTTTKKARMSAITAASAIVSGSGPDQRGLDQPGTDSKDIINQQPIQRRLWVKDRPKDWWESCNREDFPDDEFRRCFRMSKKTFNMICNELDSSVTKKNTTLRDAIPVRQRVAVCIYRLATGEPLRLVSKKFGLGISTCHKLVLEVCAAIKTVLMQKFLRWPDEETMKETKEEFEGSFGIPNIGGVMYTTHVPIIAPKVNVGLYFNKKLTERNQKTSYSVTVQGVVNSKGVFTDVCIGWPGSMQDNAVLEKSALYERAKLGNLKNIWIVGNSGYSLMDWVLVPYKHKNLTWTQHGFNERISEIEKIGKDAFSRLKGRWGCLKKRTEIKLQDLPVVLGACCVLHNICEIMNEEMDDEWKFEVFDDEMVVEDGACSLDSLKARDQIAHYLLHYGRAGTGFL