AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22543

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22543.t1 AT5G12010 58.837 430 151 4 1 417 86 502 0.0 528
MS.gene22543.t1 AT4G29780 59.620 421 164 4 1 417 122 540 5.56e-177 505
MS.gene22543.t1 AT3G55350 30.368 326 188 9 76 374 53 366 1.76e-43 157
MS.gene22543.t1 AT3G63270 29.618 314 188 10 76 363 43 349 8.39e-39 144
MS.gene22543.t1 AT3G19120 27.362 307 191 9 88 376 114 406 3.42e-24 104
MS.gene22543.t1 AT1G72275 26.797 306 162 14 88 374 92 354 5.08e-19 89.0
MS.gene22543.t1 AT1G72275 26.797 306 162 14 88 374 92 354 5.53e-19 89.0
MS.gene22543.t1 AT5G41980 22.222 306 215 6 87 389 38 323 2.43e-11 65.5

Find 96 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTTCAAATTTCCTAATT+TGG 0.108987 2.2:+47589494 None:intergenic
TAGGAAATTTGAAGAATATT+TGG 0.147062 2.2:-47589488 MS.gene22543:CDS
TACATTACACCACCAATATT+TGG 0.169757 2.2:+47589726 None:intergenic
CAAGATTTGCCTGTTGTTCT+TGG 0.174992 2.2:-47589279 MS.gene22543:CDS
AAATTCAGGTTATTCACTTA+TGG 0.234627 2.2:-47589457 MS.gene22543:CDS
ATTAGTGAAATTGAGAAAAT+TGG 0.245237 2.2:-47589369 MS.gene22543:CDS
GCTCATTATTTGTTACATTA+TGG 0.252231 2.2:-47589114 MS.gene22543:CDS
ATTTGGATAGTTGGAAATTC+AGG 0.257969 2.2:-47589471 MS.gene22543:CDS
GTCAGATTCTTATGCTTATA+TGG 0.273058 2.2:+47589420 None:intergenic
TCCTTCATTGTTTCTTCATC+TGG 0.276668 2.2:+47589783 None:intergenic
TTGAAGAATATTTGGATAGT+TGG 0.287680 2.2:-47589480 MS.gene22543:CDS
TCAGCTATTGTATCTGGTTC+TGG 0.289548 2.2:-47590218 MS.gene22543:CDS
AATGCAAGATAATGCAGTTT+TGG 0.294783 2.2:-47589541 MS.gene22543:CDS
TTCTTCCATGTAATGATTCA+TGG 0.295521 2.2:+47590322 None:intergenic
GCTGCTTCAGCTATTGTATC+TGG 0.300058 2.2:-47590224 MS.gene22543:CDS
TTATATGAGAGGGCCAAATT+AGG 0.319401 2.2:-47589507 MS.gene22543:CDS
TTTACCTTAGGTGCAATTAT+AGG 0.323232 2.2:+47589690 None:intergenic
AGTTTCGGTATTCCAAATAT+TGG 0.326727 2.2:-47589738 MS.gene22543:CDS
CAACAAGCACCAAGAACAAC+AGG 0.334974 2.2:+47589270 None:intergenic
AGGCTGGTTTCGAAGAAATT+CGG 0.335907 2.2:-47589891 MS.gene22543:CDS
ACCGCCACACGCTGGCGAAC+CGG 0.336332 2.2:+47589948 None:intergenic
TTGTTCATTGGATTCTTTGA+AGG 0.351985 2.2:-47589151 MS.gene22543:CDS
GGCTGGTTTCGAAGAAATTC+GGG 0.358679 2.2:-47589890 MS.gene22543:CDS
AGGTTATTCACTTATGGATT+GGG 0.375307 2.2:-47589451 MS.gene22543:CDS
CAGGTTATTCACTTATGGAT+TGG 0.377146 2.2:-47589452 MS.gene22543:CDS
GAAGAGTTTGAGGGCAGTTT+CGG 0.380290 2.2:-47589753 MS.gene22543:CDS
CATTATGGTCGGGCTGGTAC+TGG 0.384233 2.2:-47589099 MS.gene22543:CDS
GACGCTGCGTGATGCTATTC+CGG 0.397950 2.2:-47589967 MS.gene22543:CDS
TGAGGATGGTGCTTGTTCAT+TGG 0.398134 2.2:-47589163 MS.gene22543:CDS
TCGCCACTGGCGAACCGCTT+AGG 0.411688 2.2:-47589911 MS.gene22543:CDS
TTGCAACCGTGAAGATTTCC+CGG 0.413793 2.2:-47590081 MS.gene22543:CDS
CAACTCTCCCACCAGTCTTT+AGG 0.414933 2.2:+47590101 None:intergenic
TGAGATTATGAATGAAGAAA+TGG 0.419917 2.2:-47589229 MS.gene22543:CDS
ACCCACAGCCTACGTTGAAT+AGG 0.424840 2.2:+47590134 None:intergenic
AGCTATTCAGTAACTGTTCA+AGG 0.427841 2.2:-47589618 MS.gene22543:CDS
ATTTCCCGGATGATGAATTC+CGG 0.433406 2.2:-47590067 MS.gene22543:CDS
CACTGGCGAACCGCTTAGGC+TGG 0.434157 2.2:-47589907 MS.gene22543:CDS
TGGTCCAGACCAACGTGGTC+TGG 0.437477 2.2:-47590198 MS.gene22543:CDS
TTGTTGTTGTTTGATGATGT+TGG 0.444958 2.2:+47590273 None:intergenic
GTTTCGAAGAAATTCGGGTT+AGG 0.455239 2.2:-47589885 MS.gene22543:CDS
ATTATTTGTTACATTATGGT+CGG 0.457532 2.2:-47589110 MS.gene22543:CDS
TGGTCCAGACCACGTTGGTC+TGG 0.459547 2.2:+47590194 None:intergenic
AGCCTATTCAACGTAGGCTG+TGG 0.468864 2.2:-47590136 MS.gene22543:CDS
GATGTGTGTATTGGTTGGCC+TGG 0.470677 2.2:-47589567 MS.gene22543:CDS
CATCGCCGGAATTCATCATC+CGG 0.483016 2.2:+47590062 None:intergenic
TTATTTGTTACATTATGGTC+GGG 0.485943 2.2:-47589109 MS.gene22543:CDS
TTACTGATGTGTGTATTGGT+TGG 0.498611 2.2:-47589572 MS.gene22543:CDS
TACAAACCAACATTTACCTT+AGG 0.499068 2.2:+47589678 None:intergenic
TTCGGTATTCCAAATATTGG+TGG 0.501746 2.2:-47589735 MS.gene22543:CDS
TAGTAGGTTGAAAGGTAGGT+GGG 0.506976 2.2:-47589334 MS.gene22543:CDS
CATCATCCGGGAAATCTTCA+CGG 0.507925 2.2:+47590075 None:intergenic
CAAGGTGTGGTAAATAGTAA+AGG 0.508102 2.2:-47589600 MS.gene22543:CDS
TGAAAGACCGGCCTAAAGAC+TGG 0.509719 2.2:-47590112 MS.gene22543:CDS
ATGAAGAAATGGATGATGAA+TGG 0.517962 2.2:-47589218 MS.gene22543:CDS
GTAGGCTGTGGGTGAAAGAC+CGG 0.517962 2.2:-47590124 MS.gene22543:CDS
GCATTATCTTGCATTGAGCC+AGG 0.518982 2.2:+47589549 None:intergenic
CTGCAACGAGCTGGATTCAT+CGG 0.520703 2.2:-47590006 MS.gene22543:CDS
TGTTCCTATAATTGCACCTA+AGG 0.522840 2.2:-47589694 MS.gene22543:CDS
ATTGCACCTAAGGTAAATGT+TGG 0.524057 2.2:-47589684 MS.gene22543:CDS
TGTATATACCGCCTCGCCAC+TGG 0.524677 2.2:-47589924 MS.gene22543:CDS
CAACATGATCTGCAACGAGC+TGG 0.528812 2.2:-47590015 MS.gene22543:CDS
TTGTTACATTATGGTCGGGC+TGG 0.531532 2.2:-47589105 MS.gene22543:CDS
GGAAACGAAAGAAGAGTTTG+AGG 0.534530 2.2:-47589763 MS.gene22543:CDS
TCCGGTTCGCCAGCGTGTGG+CGG 0.537854 2.2:-47589949 MS.gene22543:CDS
TAATGCAGAAGTTTCTTCGT+TGG 0.539378 2.2:-47589806 MS.gene22543:CDS
GGATGATGAATGGAAGTTTG+AGG 0.540969 2.2:-47589208 MS.gene22543:CDS
GCCTATTCAACGTAGGCTGT+GGG 0.545772 2.2:-47590135 MS.gene22543:CDS
GCCTACGTTGAATAGGCTGC+TGG 0.548098 2.2:+47590141 None:intergenic
TAAGCGGTTCGCCAGTGGCG+AGG 0.549583 2.2:+47589913 None:intergenic
GCCAGATGAAGAAACAATGA+AGG 0.551617 2.2:-47589784 MS.gene22543:CDS
GAAACGAAAGAAGAGTTTGA+GGG 0.564474 2.2:-47589762 MS.gene22543:CDS
ATCGCCGGAATTCATCATCC+GGG 0.580423 2.2:+47590063 None:intergenic
CAGCCTAAGCGGTTCGCCAG+TGG 0.580669 2.2:+47589908 None:intergenic
TACTCATCCGAAAACATCGC+CGG 0.582531 2.2:+47590048 None:intergenic
TGATGATGAAATGGTTGTTG+AGG 0.584227 2.2:-47589181 MS.gene22543:CDS
TTCTTCGAAACCAGCCTAAG+CGG 0.591810 2.2:+47589897 None:intergenic
TTAGTAGGTTGAAAGGTAGG+TGG 0.594060 2.2:-47589335 MS.gene22543:CDS
GTAGGTTGAAAGGTAGGTGG+GGG 0.595193 2.2:-47589332 MS.gene22543:CDS
GATGAAATGGTTGTTGAGGA+TGG 0.597572 2.2:-47589177 MS.gene22543:CDS
TTCAGTAACTGTTCAAGGTG+TGG 0.599865 2.2:-47589613 MS.gene22543:CDS
AGTAGGTTGAAAGGTAGGTG+GGG 0.601658 2.2:-47589333 MS.gene22543:CDS
ACAAACCATGAATCATTACA+TGG 0.604340 2.2:-47590327 None:intergenic
GCGGTTCGCCAGTGGCGAGG+CGG 0.606304 2.2:+47589916 None:intergenic
CAACGTGGTCTGGACCAGCC+CGG 0.612903 2.2:-47590188 MS.gene22543:CDS
AGACCGGCCTAAAGACTGGT+GGG 0.625890 2.2:-47590108 MS.gene22543:CDS
TCTCCCACCAGTCTTTAGGC+CGG 0.626133 2.2:+47590105 None:intergenic
GTTGGTCTAACTGAGAGTAA+TGG 0.636217 2.2:+47590291 None:intergenic
AATCTGACGTGGACACAACA+TGG 0.637422 2.2:-47589405 MS.gene22543:CDS
TATTCCGGTTCGCCAGCGTG+TGG 0.653116 2.2:-47589952 MS.gene22543:CDS
AAGACCGGCCTAAAGACTGG+TGG 0.667437 2.2:-47590109 MS.gene22543:CDS
TAACACAAGCTTATGACACG+TGG 0.669447 2.2:+47589857 None:intergenic
CGGGCTGGTCCAGACCACGT+TGG 0.734063 2.2:+47590189 None:intergenic
GGTTCTGGTCCAGACCAACG+TGG 0.734063 2.2:-47590203 MS.gene22543:CDS
ACCAGCAGCCTATTCAACGT+AGG 0.741133 2.2:-47590142 MS.gene22543:CDS
ATATACATACCGCCACACGC+TGG 0.742881 2.2:+47589940 None:intergenic
ATAAGCATAAGAATCTGACG+TGG 0.747957 2.2:-47589416 MS.gene22543:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGTGAAATTGAGAAAAT+TGG - chr2.2:47590040-47590059 MS.gene22543:CDS 20.0%
!! ATTCTTCAAATTTCCTAATT+TGG + chr2.2:47589918-47589937 None:intergenic 20.0%
!! TAGGAAATTTGAAGAATATT+TGG - chr2.2:47589921-47589940 MS.gene22543:CDS 20.0%
!!! ATTATTTGTTACATTATGGT+CGG - chr2.2:47590299-47590318 MS.gene22543:CDS 20.0%
! TGAGATTATGAATGAAGAAA+TGG - chr2.2:47590180-47590199 MS.gene22543:CDS 25.0%
! TTGAAGAATATTTGGATAGT+TGG - chr2.2:47589929-47589948 MS.gene22543:CDS 25.0%
!! AAATTCAGGTTATTCACTTA+TGG - chr2.2:47589952-47589971 MS.gene22543:CDS 25.0%
!! AATATCTTTTGAATCTGTAC+CGG + chr2.2:47589243-47589262 None:intergenic 25.0%
!! GCTCATTATTTGTTACATTA+TGG - chr2.2:47590295-47590314 MS.gene22543:CDS 25.0%
!! TTATTTGTTACATTATGGTC+GGG - chr2.2:47590300-47590319 MS.gene22543:CDS 25.0%
!!! AAAAAAGTGCTTTATATGAG+AGG - chr2.2:47589891-47589910 MS.gene22543:CDS 25.0%
!!! AAAAAGTGCTTTATATGAGA+GGG - chr2.2:47589892-47589911 MS.gene22543:CDS 25.0%
!!! TTACTGAATAGCTAGTTTTT+TGG + chr2.2:47589784-47589803 None:intergenic 25.0%
AGGTTATTCACTTATGGATT+GGG - chr2.2:47589958-47589977 MS.gene22543:CDS 30.0%
AGTTTCGGTATTCCAAATAT+TGG - chr2.2:47589671-47589690 MS.gene22543:CDS 30.0%
ATGAAGAAATGGATGATGAA+TGG - chr2.2:47590191-47590210 MS.gene22543:CDS 30.0%
ATTTGGATAGTTGGAAATTC+AGG - chr2.2:47589938-47589957 MS.gene22543:CDS 30.0%
GTCAGATTCTTATGCTTATA+TGG + chr2.2:47589992-47590011 None:intergenic 30.0%
TACAAACCAACATTTACCTT+AGG + chr2.2:47589734-47589753 None:intergenic 30.0%
TACATTACACCACCAATATT+TGG + chr2.2:47589686-47589705 None:intergenic 30.0%
TTAACAAAAAACTCACTGAG+AGG - chr2.2:47589756-47589775 MS.gene22543:CDS 30.0%
TTGTTCATTGGATTCTTTGA+AGG - chr2.2:47590258-47590277 MS.gene22543:CDS 30.0%
TTTACCTTAGGTGCAATTAT+AGG + chr2.2:47589722-47589741 None:intergenic 30.0%
! AATGCAAGATAATGCAGTTT+TGG - chr2.2:47589868-47589887 MS.gene22543:CDS 30.0%
! ATATCTTTTGAATCTGTACC+GGG + chr2.2:47589242-47589261 None:intergenic 30.0%
! GTTTTTACTGATGTGTGTAT+TGG - chr2.2:47589833-47589852 MS.gene22543:CDS 30.0%
! TTGGTAAAGATGCTTTTAGT+AGG - chr2.2:47590059-47590078 MS.gene22543:CDS 30.0%
!! TTGTTGTTGTTTGATGATGT+TGG + chr2.2:47589139-47589158 None:intergenic 30.0%
!!! TGAGGTTTTTGATGATGAAA+TGG - chr2.2:47590219-47590238 MS.gene22543:CDS 30.0%
AGCTATTCAGTAACTGTTCA+AGG - chr2.2:47589791-47589810 MS.gene22543:CDS 35.0%
ATAAGCATAAGAATCTGACG+TGG - chr2.2:47589993-47590012 MS.gene22543:CDS 35.0%
ATTGCACCTAAGGTAAATGT+TGG - chr2.2:47589725-47589744 MS.gene22543:CDS 35.0%
CAAGGTGTGGTAAATAGTAA+AGG - chr2.2:47589809-47589828 MS.gene22543:CDS 35.0%
CAGGTTATTCACTTATGGAT+TGG - chr2.2:47589957-47589976 MS.gene22543:CDS 35.0%
GAAACGAAAGAAGAGTTTGA+GGG - chr2.2:47589647-47589666 MS.gene22543:CDS 35.0%
TAATGCAGAAGTTTCTTCGT+TGG - chr2.2:47589603-47589622 MS.gene22543:CDS 35.0%
TGTTCCTATAATTGCACCTA+AGG - chr2.2:47589715-47589734 MS.gene22543:CDS 35.0%
TTATATGAGAGGGCCAAATT+AGG - chr2.2:47589902-47589921 MS.gene22543:CDS 35.0%
TTCGGTATTCCAAATATTGG+TGG - chr2.2:47589674-47589693 MS.gene22543:CDS 35.0%
! TCCTTCATTGTTTCTTCATC+TGG + chr2.2:47589629-47589648 None:intergenic 35.0%
! TGATGATGAAATGGTTGTTG+AGG - chr2.2:47590228-47590247 MS.gene22543:CDS 35.0%
! TTACTGATGTGTGTATTGGT+TGG - chr2.2:47589837-47589856 MS.gene22543:CDS 35.0%
!! CTTTTAGTAGGTTGAAAGGT+AGG - chr2.2:47590071-47590090 MS.gene22543:CDS 35.0%
!! GATGCTTTTAGTAGGTTGAA+AGG - chr2.2:47590067-47590086 MS.gene22543:CDS 35.0%
ATTTCCCGGATGATGAATTC+CGG - chr2.2:47589342-47589361 MS.gene22543:CDS 40.0%
CAAGATTTGCCTGTTGTTCT+TGG - chr2.2:47590130-47590149 MS.gene22543:CDS 40.0%
GATGAAATGGTTGTTGAGGA+TGG - chr2.2:47590232-47590251 MS.gene22543:CDS 40.0%
GCCAGATGAAGAAACAATGA+AGG - chr2.2:47589625-47589644 MS.gene22543:CDS 40.0%
GGAAACGAAAGAAGAGTTTG+AGG - chr2.2:47589646-47589665 MS.gene22543:CDS 40.0%
GGATGATGAATGGAAGTTTG+AGG - chr2.2:47590201-47590220 MS.gene22543:CDS 40.0%
TAACACAAGCTTATGACACG+TGG + chr2.2:47589555-47589574 None:intergenic 40.0%
TTCAGTAACTGTTCAAGGTG+TGG - chr2.2:47589796-47589815 MS.gene22543:CDS 40.0%
! ATGAATTCCGGCGATGTTTT+CGG - chr2.2:47589354-47589373 MS.gene22543:CDS 40.0%
! GTTGGTCTAACTGAGAGTAA+TGG + chr2.2:47589121-47589140 None:intergenic 40.0%
! GTTTCGAAGAAATTCGGGTT+AGG - chr2.2:47589524-47589543 MS.gene22543:CDS 40.0%
! TAGTAGGTTGAAAGGTAGGT+GGG - chr2.2:47590075-47590094 MS.gene22543:CDS 40.0%
! TCAGCTATTGTATCTGGTTC+TGG - chr2.2:47589191-47589210 MS.gene22543:CDS 40.0%
! TTAGTAGGTTGAAAGGTAGG+TGG - chr2.2:47590074-47590093 MS.gene22543:CDS 40.0%
!! AGGCTGGTTTCGAAGAAATT+CGG - chr2.2:47589518-47589537 MS.gene22543:CDS 40.0%
AATCTGACGTGGACACAACA+TGG - chr2.2:47590004-47590023 MS.gene22543:CDS 45.0%
CAACAAGCACCAAGAACAAC+AGG + chr2.2:47590142-47590161 None:intergenic 45.0%
CATCATCCGGGAAATCTTCA+CGG + chr2.2:47589337-47589356 None:intergenic 45.0%
GAAGAGTTTGAGGGCAGTTT+CGG - chr2.2:47589656-47589675 MS.gene22543:CDS 45.0%
GCATTATCTTGCATTGAGCC+AGG + chr2.2:47589863-47589882 None:intergenic 45.0%
GCTGCTTCAGCTATTGTATC+TGG - chr2.2:47589185-47589204 MS.gene22543:CDS 45.0%
TACTCATCCGAAAACATCGC+CGG + chr2.2:47589364-47589383 None:intergenic 45.0%
TGAGGATGGTGCTTGTTCAT+TGG - chr2.2:47590246-47590265 MS.gene22543:CDS 45.0%
TTCTTCGAAACCAGCCTAAG+CGG + chr2.2:47589515-47589534 None:intergenic 45.0%
TTGCAACCGTGAAGATTTCC+CGG - chr2.2:47589328-47589347 MS.gene22543:CDS 45.0%
TTGTTACATTATGGTCGGGC+TGG - chr2.2:47590304-47590323 MS.gene22543:CDS 45.0%
! AGTAGGTTGAAAGGTAGGTG+GGG - chr2.2:47590076-47590095 MS.gene22543:CDS 45.0%
!! GGCTGGTTTCGAAGAAATTC+GGG - chr2.2:47589519-47589538 MS.gene22543:CDS 45.0%
ACCAGCAGCCTATTCAACGT+AGG - chr2.2:47589267-47589286 MS.gene22543:CDS 50.0%
ACCCACAGCCTACGTTGAAT+AGG + chr2.2:47589278-47589297 None:intergenic 50.0%
AGCCTATTCAACGTAGGCTG+TGG - chr2.2:47589273-47589292 MS.gene22543:CDS 50.0%
ATATACATACCGCCACACGC+TGG + chr2.2:47589472-47589491 None:intergenic 50.0%
ATCGCCGGAATTCATCATCC+GGG + chr2.2:47589349-47589368 None:intergenic 50.0%
CAACATGATCTGCAACGAGC+TGG - chr2.2:47589394-47589413 MS.gene22543:CDS 50.0%
CAACTCTCCCACCAGTCTTT+AGG + chr2.2:47589311-47589330 None:intergenic 50.0%
CATCGCCGGAATTCATCATC+CGG + chr2.2:47589350-47589369 None:intergenic 50.0%
CTGCAACGAGCTGGATTCAT+CGG - chr2.2:47589403-47589422 MS.gene22543:CDS 50.0%
GCCTATTCAACGTAGGCTGT+GGG - chr2.2:47589274-47589293 MS.gene22543:CDS 50.0%
TGAAAGACCGGCCTAAAGAC+TGG - chr2.2:47589297-47589316 MS.gene22543:CDS 50.0%
! CTTTTGAATCTGTACCGGGC+TGG + chr2.2:47589238-47589257 None:intergenic 50.0%
! GTAGGTTGAAAGGTAGGTGG+GGG - chr2.2:47590077-47590096 MS.gene22543:CDS 50.0%
!! GATGTGTGTATTGGTTGGCC+TGG - chr2.2:47589842-47589861 MS.gene22543:CDS 50.0%
!! GGTGGGGGTGTTTGAAGAAA+AGG - chr2.2:47590092-47590111 MS.gene22543:CDS 50.0%
AAGACCGGCCTAAAGACTGG+TGG - chr2.2:47589300-47589319 MS.gene22543:CDS 55.0%
AGACCGGCCTAAAGACTGGT+GGG - chr2.2:47589301-47589320 MS.gene22543:CDS 55.0%
GACGCTGCGTGATGCTATTC+CGG - chr2.2:47589442-47589461 MS.gene22543:CDS 55.0%
GCCTACGTTGAATAGGCTGC+TGG + chr2.2:47589271-47589290 None:intergenic 55.0%
GTAGGCTGTGGGTGAAAGAC+CGG - chr2.2:47589285-47589304 MS.gene22543:CDS 55.0%
TCTCCCACCAGTCTTTAGGC+CGG + chr2.2:47589307-47589326 None:intergenic 55.0%
TGTATATACCGCCTCGCCAC+TGG - chr2.2:47589485-47589504 MS.gene22543:CDS 55.0%
!! CATTATGGTCGGGCTGGTAC+TGG - chr2.2:47590310-47590329 MS.gene22543:CDS 55.0%
TGGTCCAGACCAACGTGGTC+TGG - chr2.2:47589211-47589230 MS.gene22543:CDS 60.0%
TGGTCCAGACCACGTTGGTC+TGG + chr2.2:47589218-47589237 None:intergenic 60.0%
! GGTTCTGGTCCAGACCAACG+TGG - chr2.2:47589206-47589225 MS.gene22543:CDS 60.0%
! TATTCCGGTTCGCCAGCGTG+TGG - chr2.2:47589457-47589476 MS.gene22543:CDS 60.0%
CAACGTGGTCTGGACCAGCC+CGG - chr2.2:47589221-47589240 MS.gene22543:CDS 65.0%
CACTGGCGAACCGCTTAGGC+TGG - chr2.2:47589502-47589521 MS.gene22543:CDS 65.0%
CAGCCTAAGCGGTTCGCCAG+TGG + chr2.2:47589504-47589523 None:intergenic 65.0%
TAAGCGGTTCGCCAGTGGCG+AGG + chr2.2:47589499-47589518 None:intergenic 65.0%
TCGCCACTGGCGAACCGCTT+AGG - chr2.2:47589498-47589517 MS.gene22543:CDS 65.0%
ACCGCCACACGCTGGCGAAC+CGG + chr2.2:47589464-47589483 None:intergenic 70.0%
! TCCGGTTCGCCAGCGTGTGG+CGG - chr2.2:47589460-47589479 MS.gene22543:CDS 70.0%
!! CGGGCTGGTCCAGACCACGT+TGG + chr2.2:47589223-47589242 None:intergenic 70.0%
GCGGTTCGCCAGTGGCGAGG+CGG + chr2.2:47589496-47589515 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 47589089 47590342 47589089 ID=MS.gene22543
chr2.2 mRNA 47589089 47590342 47589089 ID=MS.gene22543.t1;Parent=MS.gene22543
chr2.2 exon 47589089 47590342 47589089 ID=MS.gene22543.t1.exon1;Parent=MS.gene22543.t1
chr2.2 CDS 47589089 47590342 47589089 ID=cds.MS.gene22543.t1;Parent=MS.gene22543.t1
Gene Sequence

>MS.gene22543

ATGAATCATTACATGGAAGAACTTCAAAACCATTACTCTCAGTTAGACCAACATCATCAAACAACAACAAAAAAAGCTCGTATGTCTGCTATTACAGCTGCTTCAGCTATTGTATCTGGTTCTGGTCCAGACCAACGTGGTCTGGACCAGCCCGGTACAGATTCAAAAGATATTATCAACCAGCAGCCTATTCAACGTAGGCTGTGGGTGAAAGACCGGCCTAAAGACTGGTGGGAGAGTTGCAACCGTGAAGATTTCCCGGATGATGAATTCCGGCGATGTTTTCGGATGAGTAAAAAAACATTCAACATGATCTGCAACGAGCTGGATTCATCGGTAACGAAGAAAAACACGACGCTGCGTGATGCTATTCCGGTTCGCCAGCGTGTGGCGGTATGTATATACCGCCTCGCCACTGGCGAACCGCTTAGGCTGGTTTCGAAGAAATTCGGGTTAGGAATATCCACGTGTCATAAGCTTGTGTTAGAAGTTTGTGCAGCTATTAAAACAGTTTTAATGCAGAAGTTTCTTCGTTGGCCAGATGAAGAAACAATGAAGGAAACGAAAGAAGAGTTTGAGGGCAGTTTCGGTATTCCAAATATTGGTGGTGTAATGTATACTACTCATGTTCCTATAATTGCACCTAAGGTAAATGTTGGTTTGTATTTTAACAAAAAACTCACTGAGAGGAACCAAAAAACTAGCTATTCAGTAACTGTTCAAGGTGTGGTAAATAGTAAAGGTGTTTTTACTGATGTGTGTATTGGTTGGCCTGGCTCAATGCAAGATAATGCAGTTTTGGAAAAAAGTGCTTTATATGAGAGGGCCAAATTAGGAAATTTGAAGAATATTTGGATAGTTGGAAATTCAGGTTATTCACTTATGGATTGGGTTTTAGTGCCATATAAGCATAAGAATCTGACGTGGACACAACATGGTTTTAATGAGAGAATTAGTGAAATTGAGAAAATTGGTAAAGATGCTTTTAGTAGGTTGAAAGGTAGGTGGGGGTGTTTGAAGAAAAGGACTGAAATTAAGTTGCAAGATTTGCCTGTTGTTCTTGGTGCTTGTTGTGTGCTTCATAATATTTGTGAGATTATGAATGAAGAAATGGATGATGAATGGAAGTTTGAGGTTTTTGATGATGAAATGGTTGTTGAGGATGGTGCTTGTTCATTGGATTCTTTGAAGGCTAGAGATCAAATTGCTCATTATTTGTTACATTATGGTCGGGCTGGTACTGGATTTTTATAG
Protein sequence

>MS.gene22543.t1

MNHYMEELQNHYSQLDQHHQTTTKKARMSAITAASAIVSGSGPDQRGLDQPGTDSKDIINQQPIQRRLWVKDRPKDWWESCNREDFPDDEFRRCFRMSKKTFNMICNELDSSVTKKNTTLRDAIPVRQRVAVCIYRLATGEPLRLVSKKFGLGISTCHKLVLEVCAAIKTVLMQKFLRWPDEETMKETKEEFEGSFGIPNIGGVMYTTHVPIIAPKVNVGLYFNKKLTERNQKTSYSVTVQGVVNSKGVFTDVCIGWPGSMQDNAVLEKSALYERAKLGNLKNIWIVGNSGYSLMDWVLVPYKHKNLTWTQHGFNERISEIEKIGKDAFSRLKGRWGCLKKRTEIKLQDLPVVLGACCVLHNICEIMNEEMDDEWKFEVFDDEMVVEDGACSLDSLKARDQIAHYLLHYGRAGTGFL