AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22759


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22759.t1 MTR_7g117475 95.122 246 9 2 3 245 69 314 3.26e-171 474
MS.gene22759.t1 MTR_8g023760 70.518 251 62 3 3 245 83 329 1.78e-127 364
MS.gene22759.t1 MTR_8g107030 34.444 180 100 7 57 227 87 257 1.20e-14 72.4
MS.gene22759.t1 MTR_8g107030 33.702 181 102 7 57 228 118 289 2.07e-14 72.0
MS.gene22759.t1 MTR_8g107030 33.143 175 99 7 57 222 87 252 4.45e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22759.t1 AT2G46890 64.940 251 76 3 3 245 76 322 7.92e-119 342
MS.gene22759.t1 AT1G18180 33.171 205 114 8 30 223 61 253 1.79e-15 74.3
MS.gene22759.t1 AT1G18180 32.683 205 115 8 30 223 61 253 2.05e-15 74.3
MS.gene22759.t1 AT1G73650 32.258 217 129 9 30 238 61 267 9.36e-13 66.6
MS.gene22759.t1 AT1G73650 32.258 217 129 9 30 238 61 267 9.95e-13 67.0
MS.gene22759.t1 AT1G73650 32.258 217 129 9 30 238 61 267 1.08e-12 66.6
MS.gene22759.t1 AT1G73650 35.099 151 89 5 96 238 36 185 1.73e-12 65.1
MS.gene22759.t1 AT1G73650 35.099 151 89 5 96 238 36 185 2.11e-12 64.7

Find 75 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGCTCACTATGATTTC+TGG 0.120398 7.3:+252060 MS.gene22759:CDS
TGCAGATGATTGACCCTTAT+TGG 0.153787 7.3:+251994 MS.gene22759:CDS
GGGCATGGGTGGACCTTTAT+AGG 0.223333 7.3:+252527 MS.gene22759:CDS
TACTGAGTGGATAACATAAA+AGG 0.250782 7.3:-252288 None:intergenic
TGGTGGGGACTTGTAGTATT+TGG 0.269007 7.3:+252494 MS.gene22759:CDS
CATTCTTTGTTCCACAAGTT+TGG 0.288006 7.3:-252585 None:intergenic
GCTGTTGTTGTGTGTGTATC+TGG 0.295142 7.3:+252341 MS.gene22759:CDS
ACTGATATGATTCAACAATA+TGG 0.297749 7.3:+252191 MS.gene22759:CDS
ACAGGTATCACTGTCCAATA+AGG 0.308159 7.3:-252008 None:intergenic
ATTGCTCAACACAATGTGTT+TGG 0.338794 7.3:+252553 MS.gene22759:CDS
TTGGTTGGAACTTGGGGCAT+GGG 0.350925 7.3:+252513 MS.gene22759:CDS
TAAATCAGCCTTTGAACATC+TGG 0.357916 7.3:+252309 MS.gene22759:CDS
AGCTGCTCACCAAAGTAATT+AGG 0.361585 7.3:-252467 None:intergenic
CCTCACCAGTTATTGCTCAT+TGG 0.368116 7.3:+252254 MS.gene22759:CDS
AAGGGTGGGCTGCATAATAA+TGG 0.377206 7.3:-252040 None:intergenic
TGTAGTATTTGGTTGGAACT+TGG 0.385747 7.3:+252505 MS.gene22759:CDS
TTATTATGCAGCCCACCCTT+TGG 0.386930 7.3:+252043 MS.gene22759:CDS
TTGCTCAACACAATGTGTTT+GGG 0.407007 7.3:+252554 MS.gene22759:CDS
TTTGGTTGGAACTTGGGGCA+TGG 0.421477 7.3:+252512 MS.gene22759:CDS
GTAGTATTTGGTTGGAACTT+GGG 0.421705 7.3:+252506 MS.gene22759:CDS
ACAAAAGAGTAGAAGCATAT+AGG 0.425663 7.3:+252618 MS.gene22759:CDS
TACTCCACACCCAAGTCAAC+AGG 0.431420 7.3:-252100 None:intergenic
GCCTTTGAACATCTGGGACT+TGG 0.435472 7.3:+252316 MS.gene22759:CDS
AATGGGGTGCAAGGGAAGAT+TGG 0.438963 7.3:+252162 MS.gene22759:CDS
TTTGTGAATAACAATGGTAA+TGG 0.445517 7.3:+252404 MS.gene22759:CDS
ACAATCCAATGAGCAATAAC+TGG 0.447606 7.3:-252259 None:intergenic
TAGTATTTGGTTGGAACTTG+GGG 0.450578 7.3:+252507 MS.gene22759:CDS
GTTGGAACTTGGGGCATGGG+TGG 0.451857 7.3:+252516 MS.gene22759:CDS
AGGTAGGTAGATGGAGAAGA+AGG 0.452132 7.3:-252234 None:intergenic
GTTGAGCAATGCTCCTATAA+AGG 0.453733 7.3:-252540 None:intergenic
ATTACTTTGGTGAGCAGCTA+TGG 0.459668 7.3:+252471 MS.gene22759:CDS
GGGGACTTGTAGTATTTGGT+TGG 0.460375 7.3:+252498 MS.gene22759:CDS
ACTCGGCATCCTAATTACTT+TGG 0.476296 7.3:+252458 MS.gene22759:CDS
CAGACGAGAGAAGTGGCAAT+GGG 0.479915 7.3:+252145 MS.gene22759:CDS
ACTATGATTTCTGGAGATCA+AGG 0.489125 7.3:+252069 MS.gene22759:CDS
TGGTGAGCAGCTATGGTGGT+GGG 0.489748 7.3:+252478 MS.gene22759:CDS
TAACTGGTGAGGTAGGTAGA+TGG 0.495738 7.3:-252243 None:intergenic
ATCAGAACACAACTTCTGTA+TGG 0.498222 7.3:+252645 MS.gene22759:CDS
ACTGAGTGGATAACATAAAA+GGG 0.503941 7.3:-252287 None:intergenic
TTGGTGAGCAGCTATGGTGG+TGG 0.505228 7.3:+252477 MS.gene22759:CDS
CAGGTATCACTGTCCAATAA+GGG 0.506881 7.3:-252007 None:intergenic
AGATGAGTTGAACTTGAAGC+AGG 0.514693 7.3:-252672 None:intergenic
AGAAGTGGCAATGGGGTGCA+AGG 0.516645 7.3:+252153 MS.gene22759:CDS
AACAATATGGAAAACATTGG+TGG 0.523523 7.3:+252204 MS.gene22759:CDS
TGATCCTTGAGAGTGGTTTG+TGG 0.530278 7.3:+252429 MS.gene22759:CDS
AATATGGAAAACATTGGTGG+TGG 0.539715 7.3:+252207 MS.gene22759:CDS
TAATAATGGACAAGCATCAC+AGG 0.547440 7.3:-252026 None:intergenic
GGTGAGCAGCTATGGTGGTG+GGG 0.554190 7.3:+252479 MS.gene22759:CDS
TGATCCTGTTGACTTGGGTG+TGG 0.558189 7.3:+252096 MS.gene22759:CDS
GGGTTATGTGACCAAACTTG+TGG 0.558732 7.3:+252574 MS.gene22759:CDS
GGATAGTGATCCTGTTGACT+TGG 0.567382 7.3:+252090 MS.gene22759:CDS
CAGAAATCATAGTGAGCCAA+AGG 0.576012 7.3:-252059 None:intergenic
TCTCTCGTCTGAAGTAGTTG+TGG 0.584105 7.3:-252133 None:intergenic
AAATCAGCCTTTGAACATCT+GGG 0.586715 7.3:+252310 MS.gene22759:CDS
ACCAAGTCCCAGATGTTCAA+AGG 0.590896 7.3:-252317 None:intergenic
GATGAGTTGAACTTGAAGCA+GGG 0.598199 7.3:-252671 None:intergenic
TGAGCAATAACTGGTGAGGT+AGG 0.598678 7.3:-252250 None:intergenic
AGAAATCATAGTGAGCCAAA+GGG 0.603318 7.3:-252058 None:intergenic
GAAGTGGCAATGGGGTGCAA+GGG 0.603591 7.3:+252154 MS.gene22759:CDS
ATCATAGTGAGCCAAAGGGT+GGG 0.605285 7.3:-252054 None:intergenic
TTCAACAATATGGAAAACAT+TGG 0.608096 7.3:+252201 MS.gene22759:CDS
TCAGACGAGAGAAGTGGCAA+TGG 0.611058 7.3:+252144 MS.gene22759:CDS
ATGAGTTGAACTTGAAGCAG+GGG 0.615221 7.3:-252670 None:intergenic
AATCATAGTGAGCCAAAGGG+TGG 0.615234 7.3:-252055 None:intergenic
AGTACCACAAACCACTCTCA+AGG 0.625195 7.3:-252433 None:intergenic
ATATGGAAAACATTGGTGGT+GGG 0.626188 7.3:+252208 MS.gene22759:CDS
TCAAAGGCTGATTTACTGAG+TGG 0.626720 7.3:-252301 None:intergenic
GTGGTTTGTGGTACTATACT+CGG 0.626740 7.3:+252441 MS.gene22759:CDS
ACTACTTCAGACGAGAGAAG+TGG 0.633464 7.3:+252138 MS.gene22759:CDS
GATAGTGATCCTGTTGACTT+GGG 0.636502 7.3:+252091 MS.gene22759:CDS
CCAATGAGCAATAACTGGTG+AGG 0.640552 7.3:-252254 None:intergenic
ACTTTGGTGAGCAGCTATGG+TGG 0.658770 7.3:+252474 MS.gene22759:CDS
TATCTGCAAAGTATGCTATG+AGG 0.672642 7.3:-252367 None:intergenic
AATGGTATGATCCTTGAGAG+TGG 0.682971 7.3:+252422 MS.gene22759:CDS
AGACGAGAGAAGTGGCAATG+GGG 0.720125 7.3:+252146 MS.gene22759:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACAATTTTGTGAATAACAA+TGG + chr7.3:252398-252417 MS.gene22759:CDS 20.0%
! ACTGATATGATTCAACAATA+TGG + chr7.3:252191-252210 MS.gene22759:CDS 25.0%
! TTCAACAATATGGAAAACAT+TGG + chr7.3:252201-252220 MS.gene22759:CDS 25.0%
! TTTGTGAATAACAATGGTAA+TGG + chr7.3:252404-252423 MS.gene22759:CDS 25.0%
AACAATATGGAAAACATTGG+TGG + chr7.3:252204-252223 MS.gene22759:CDS 30.0%
ACTGAGTGGATAACATAAAA+GGG - chr7.3:252290-252309 None:intergenic 30.0%
TACTGAGTGGATAACATAAA+AGG - chr7.3:252291-252310 None:intergenic 30.0%
! ACAAAAGAGTAGAAGCATAT+AGG + chr7.3:252618-252637 MS.gene22759:CDS 30.0%
!!! GCATTCTTAGTTTTTTTGAG+TGG - chr7.3:252707-252726 None:intergenic 30.0%
AAATCAGCCTTTGAACATCT+GGG + chr7.3:252310-252329 MS.gene22759:CDS 35.0%
AATATGGAAAACATTGGTGG+TGG + chr7.3:252207-252226 MS.gene22759:CDS 35.0%
ACAATCCAATGAGCAATAAC+TGG - chr7.3:252262-252281 None:intergenic 35.0%
AGAAATCATAGTGAGCCAAA+GGG - chr7.3:252061-252080 None:intergenic 35.0%
ATCAGAACACAACTTCTGTA+TGG + chr7.3:252645-252664 MS.gene22759:CDS 35.0%
ATTGCTCAACACAATGTGTT+TGG + chr7.3:252553-252572 MS.gene22759:CDS 35.0%
CATTCTTTGTTCCACAAGTT+TGG - chr7.3:252588-252607 None:intergenic 35.0%
GTAGTATTTGGTTGGAACTT+GGG + chr7.3:252506-252525 MS.gene22759:CDS 35.0%
TAAATCAGCCTTTGAACATC+TGG + chr7.3:252309-252328 MS.gene22759:CDS 35.0%
TAGTATTTGGTTGGAACTTG+GGG + chr7.3:252507-252526 MS.gene22759:CDS 35.0%
TATCTGCAAAGTATGCTATG+AGG - chr7.3:252370-252389 None:intergenic 35.0%
TGTAGTATTTGGTTGGAACT+TGG + chr7.3:252505-252524 MS.gene22759:CDS 35.0%
TTGCTCAACACAATGTGTTT+GGG + chr7.3:252554-252573 MS.gene22759:CDS 35.0%
! ACTATGATTTCTGGAGATCA+AGG + chr7.3:252069-252088 MS.gene22759:CDS 35.0%
! ATATGGAAAACATTGGTGGT+GGG + chr7.3:252208-252227 MS.gene22759:CDS 35.0%
!! TAATAATGGACAAGCATCAC+AGG - chr7.3:252029-252048 None:intergenic 35.0%
AATGGTATGATCCTTGAGAG+TGG + chr7.3:252422-252441 MS.gene22759:CDS 40.0%
ACAGGTATCACTGTCCAATA+AGG - chr7.3:252011-252030 None:intergenic 40.0%
ACTCGGCATCCTAATTACTT+TGG + chr7.3:252458-252477 MS.gene22759:CDS 40.0%
AGCTGCTCACCAAAGTAATT+AGG - chr7.3:252470-252489 None:intergenic 40.0%
ATTACTTTGGTGAGCAGCTA+TGG + chr7.3:252471-252490 MS.gene22759:CDS 40.0%
CAGAAATCATAGTGAGCCAA+AGG - chr7.3:252062-252081 None:intergenic 40.0%
CAGGTATCACTGTCCAATAA+GGG - chr7.3:252010-252029 None:intergenic 40.0%
GTTGAGCAATGCTCCTATAA+AGG - chr7.3:252543-252562 None:intergenic 40.0%
TGCAGATGATTGACCCTTAT+TGG + chr7.3:251994-252013 MS.gene22759:CDS 40.0%
! AGATGAGTTGAACTTGAAGC+AGG - chr7.3:252675-252694 None:intergenic 40.0%
! ATGAGTTGAACTTGAAGCAG+GGG - chr7.3:252673-252692 None:intergenic 40.0%
! CTTTGGCTCACTATGATTTC+TGG + chr7.3:252060-252079 MS.gene22759:CDS 40.0%
! GATGAGTTGAACTTGAAGCA+GGG - chr7.3:252674-252693 None:intergenic 40.0%
! GTGGTTTGTGGTACTATACT+CGG + chr7.3:252441-252460 MS.gene22759:CDS 40.0%
! TCAAAGGCTGATTTACTGAG+TGG - chr7.3:252304-252323 None:intergenic 40.0%
!! GATAGTGATCCTGTTGACTT+GGG + chr7.3:252091-252110 MS.gene22759:CDS 40.0%
AATCATAGTGAGCCAAAGGG+TGG - chr7.3:252058-252077 None:intergenic 45.0%
ACCAAGTCCCAGATGTTCAA+AGG - chr7.3:252320-252339 None:intergenic 45.0%
ACTACTTCAGACGAGAGAAG+TGG + chr7.3:252138-252157 MS.gene22759:CDS 45.0%
AGGTAGGTAGATGGAGAAGA+AGG - chr7.3:252237-252256 None:intergenic 45.0%
AGTACCACAAACCACTCTCA+AGG - chr7.3:252436-252455 None:intergenic 45.0%
ATCATAGTGAGCCAAAGGGT+GGG - chr7.3:252057-252076 None:intergenic 45.0%
CCAATGAGCAATAACTGGTG+AGG - chr7.3:252257-252276 None:intergenic 45.0%
CCTCACCAGTTATTGCTCAT+TGG + chr7.3:252254-252273 MS.gene22759:CDS 45.0%
GCTGTTGTTGTGTGTGTATC+TGG + chr7.3:252341-252360 MS.gene22759:CDS 45.0%
GGGGACTTGTAGTATTTGGT+TGG + chr7.3:252498-252517 MS.gene22759:CDS 45.0%
GGGTTATGTGACCAAACTTG+TGG + chr7.3:252574-252593 MS.gene22759:CDS 45.0%
TAACTGGTGAGGTAGGTAGA+TGG - chr7.3:252246-252265 None:intergenic 45.0%
TGAGCAATAACTGGTGAGGT+AGG - chr7.3:252253-252272 None:intergenic 45.0%
TGATCCTTGAGAGTGGTTTG+TGG + chr7.3:252429-252448 MS.gene22759:CDS 45.0%
TGGTGGGGACTTGTAGTATT+TGG + chr7.3:252494-252513 MS.gene22759:CDS 45.0%
TTATTATGCAGCCCACCCTT+TGG + chr7.3:252043-252062 MS.gene22759:CDS 45.0%
! GGATAGTGATCCTGTTGACT+TGG + chr7.3:252090-252109 MS.gene22759:CDS 45.0%
! TCTCTCGTCTGAAGTAGTTG+TGG - chr7.3:252136-252155 None:intergenic 45.0%
!! AAGGGTGGGCTGCATAATAA+TGG - chr7.3:252043-252062 None:intergenic 45.0%
AATGGGGTGCAAGGGAAGAT+TGG + chr7.3:252162-252181 MS.gene22759:CDS 50.0%
ACTTTGGTGAGCAGCTATGG+TGG + chr7.3:252474-252493 MS.gene22759:CDS 50.0%
AGACGAGAGAAGTGGCAATG+GGG + chr7.3:252146-252165 MS.gene22759:CDS 50.0%
CAGACGAGAGAAGTGGCAAT+GGG + chr7.3:252145-252164 MS.gene22759:CDS 50.0%
TACTCCACACCCAAGTCAAC+AGG - chr7.3:252103-252122 None:intergenic 50.0%
TCAGACGAGAGAAGTGGCAA+TGG + chr7.3:252144-252163 MS.gene22759:CDS 50.0%
TTGGTTGGAACTTGGGGCAT+GGG + chr7.3:252513-252532 MS.gene22759:CDS 50.0%
TTTGGTTGGAACTTGGGGCA+TGG + chr7.3:252512-252531 MS.gene22759:CDS 50.0%
! GCCTTTGAACATCTGGGACT+TGG + chr7.3:252316-252335 MS.gene22759:CDS 50.0%
!! TGATCCTGTTGACTTGGGTG+TGG + chr7.3:252096-252115 MS.gene22759:CDS 50.0%
AGAAGTGGCAATGGGGTGCA+AGG + chr7.3:252153-252172 MS.gene22759:CDS 55.0%
GAAGTGGCAATGGGGTGCAA+GGG + chr7.3:252154-252173 MS.gene22759:CDS 55.0%
GGGCATGGGTGGACCTTTAT+AGG + chr7.3:252527-252546 MS.gene22759:CDS 55.0%
TGGTGAGCAGCTATGGTGGT+GGG + chr7.3:252478-252497 MS.gene22759:CDS 55.0%
TTGGTGAGCAGCTATGGTGG+TGG + chr7.3:252477-252496 MS.gene22759:CDS 55.0%
GTTGGAACTTGGGGCATGGG+TGG + chr7.3:252516-252535 MS.gene22759:CDS 60.0%
! GGTGAGCAGCTATGGTGGTG+GGG + chr7.3:252479-252498 MS.gene22759:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 251993 252730 251993 ID=MS.gene22759
chr7.3 mRNA 251993 252730 251993 ID=MS.gene22759.t1;Parent=MS.gene22759
chr7.3 exon 251993 252730 251993 ID=MS.gene22759.t1.exon1;Parent=MS.gene22759.t1
chr7.3 CDS 251993 252730 251993 ID=cds.MS.gene22759.t1;Parent=MS.gene22759.t1
Gene Sequence

>MS.gene22759

ATGCAGATGATTGACCCTTATTGGACAGTGATACCTGTGATGCTTGTCCATTATTATGCAGCCCACCCTTTGGCTCACTATGATTTCTGGAGATCAAGGATAGTGATCCTGTTGACTTGGGTGTGGAGTATTAGACTCATCCACAACTACTTCAGACGAGAGAAGTGGCAATGGGGTGCAAGGGAAGATTGGCGTTTCACTGATATGATTCAACAATATGGAAAACATTGGTGGTGGGCTTCCTTCTTCTCCATCTACCTACCTCACCAGTTATTGCTCATTGGATTGTCACTTCCCTTTTATGTTATCCACTCAGTAAATCAGCCTTTGAACATCTGGGACTTGGTAGCTGTTGTTGTGTGTGTATCTGGCATCCTCATAGCATACTTTGCAGATAATCAGCTTTACAATTTTGTGAATAACAATGGTAATGGTATGATCCTTGAGAGTGGTTTGTGGTACTATACTCGGCATCCTAATTACTTTGGTGAGCAGCTATGGTGGTGGGGACTTGTAGTATTTGGTTGGAACTTGGGGCATGGGTGGACCTTTATAGGAGCATTGCTCAACACAATGTGTTTGGGTTATGTGACCAAACTTGTGGAACAAAGAATGTTGAAACAAGACAAAAGAGTAGAAGCATATAGGAAGTATCAGAACACAACTTCTGTATGGATCCCCTGCTTCAAGTTCAACTCATCTGCTAATTTACCACTCAAAAAAACTAAGAATGCCTGA

Protein sequence

>MS.gene22759.t1

MQMIDPYWTVIPVMLVHYYAAHPLAHYDFWRSRIVILLTWVWSIRLIHNYFRREKWQWGAREDWRFTDMIQQYGKHWWWASFFSIYLPHQLLLIGLSLPFYVIHSVNQPLNIWDLVAVVVCVSGILIAYFADNQLYNFVNNNGNGMILESGLWYYTRHPNYFGEQLWWWGLVVFGWNLGHGWTFIGALLNTMCLGYVTKLVEQRMLKQDKRVEAYRKYQNTTSVWIPCFKFNSSANLPLKKTKNA