AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25138


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25138.t1 MTR_8g020610 95.169 207 10 0 1 207 1 207 3.12e-143 397
MS.gene25138.t1 MTR_8g020590 58.373 209 84 2 1 207 1 208 1.07e-80 239
MS.gene25138.t1 MTR_8g020630 56.459 209 87 3 1 207 3 209 2.52e-76 228
MS.gene25138.t1 MTR_8g020670 56.796 206 85 3 4 207 6 209 7.85e-76 227
MS.gene25138.t1 MTR_2g044040 54.545 209 91 3 1 207 3 209 1.07e-72 219
MS.gene25138.t1 MTR_2g041570 51.196 209 98 2 1 207 4 210 1.13e-72 219
MS.gene25138.t1 MTR_8g020650 58.031 193 77 3 17 207 19 209 1.62e-72 219
MS.gene25138.t1 MTR_2g041580 51.196 209 98 2 1 207 4 210 4.58e-72 218
MS.gene25138.t1 MTR_2g041550 51.196 209 98 2 1 207 4 210 1.83e-71 216
MS.gene25138.t1 MTR_2g045100 54.369 206 90 3 4 207 6 209 6.16e-71 214
MS.gene25138.t1 MTR_3g019710 54.067 209 92 3 1 207 3 209 2.39e-69 211
MS.gene25138.t1 MTR_2g036120 52.404 208 95 3 1 206 3 208 1.72e-68 208
MS.gene25138.t1 MTR_8g020770 50.725 207 98 3 4 207 7 212 2.38e-66 203
MS.gene25138.t1 MTR_0284s0010 52.632 209 95 3 1 207 3 209 4.69e-66 202
MS.gene25138.t1 MTR_3g019650 52.913 206 92 4 4 207 6 208 9.64e-66 201
MS.gene25138.t1 MTR_4g010340 52.913 206 90 5 4 207 5 205 4.35e-65 199
MS.gene25138.t1 MTR_5g031460 50.718 209 99 3 1 207 3 209 5.45e-65 199
MS.gene25138.t1 MTR_3g019680 52.427 206 93 4 4 207 6 208 1.33e-64 199
MS.gene25138.t1 MTR_7g011340 53.093 194 87 3 16 207 18 209 3.00e-61 190
MS.gene25138.t1 MTR_2g019780 49.048 210 100 5 2 207 6 212 1.71e-59 186
MS.gene25138.t1 MTR_3g073170 51.337 187 87 4 22 207 27 210 1.80e-59 186
MS.gene25138.t1 MTR_8g020760 49.519 208 101 3 2 207 6 211 5.72e-58 182
MS.gene25138.t1 MTR_8g020750 45.894 207 108 3 4 207 7 212 1.35e-57 181
MS.gene25138.t1 MTR_3g019810 46.305 203 99 4 1 201 109 303 2.82e-52 170
MS.gene25138.t1 MTR_8g020763 46.995 183 94 2 27 207 9 190 1.43e-51 165
MS.gene25138.t1 MTR_1g060120 36.979 192 107 5 20 200 30 218 4.62e-36 126
MS.gene25138.t1 MTR_1g085140 37.838 185 109 4 22 201 30 213 3.31e-34 121
MS.gene25138.t1 MTR_4g027415 49.606 127 60 3 16 140 18 142 1.11e-32 115
MS.gene25138.t1 MTR_1g112200 35.263 190 114 4 20 206 27 210 2.36e-32 116
MS.gene25138.t1 MTR_2g072560 52.252 111 50 2 4 112 6 115 2.38e-32 114
MS.gene25138.t1 MTR_7g111240 37.975 158 96 1 22 177 26 183 1.81e-30 111
MS.gene25138.t1 MTR_6g005360 39.474 190 105 6 22 203 28 215 4.88e-30 110
MS.gene25138.t1 MTR_6g005330 39.474 190 105 6 22 203 28 215 6.65e-30 110
MS.gene25138.t1 MTR_6g005310 39.474 190 105 6 22 203 28 215 8.68e-30 110
MS.gene25138.t1 MTR_7g111240 37.975 158 93 2 22 177 26 180 2.10e-29 108
MS.gene25138.t1 MTR_5g036570 33.333 189 121 3 22 205 33 221 3.20e-29 108
MS.gene25138.t1 MTR_1g079490 37.838 185 104 5 3 177 6 189 3.35e-29 108
MS.gene25138.t1 MTR_6g005340 38.947 190 106 6 22 203 28 215 5.99e-29 107
MS.gene25138.t1 MTR_2g031270 35.638 188 112 4 22 203 29 213 1.86e-28 106
MS.gene25138.t1 MTR_2g030855 34.555 191 116 4 19 203 25 212 5.39e-28 105
MS.gene25138.t1 MTR_1g023770 35.468 203 118 5 8 203 13 209 7.28e-28 104
MS.gene25138.t1 MTR_3g019610 50.980 102 47 2 4 103 6 106 1.10e-27 101
MS.gene25138.t1 MTR_1g085500 32.240 183 120 2 22 200 29 211 1.27e-27 104
MS.gene25138.t1 MTR_2g019250 34.286 210 125 7 1 200 7 213 9.30e-27 102
MS.gene25138.t1 MTR_4g017040 35.829 187 109 6 1 179 7 190 1.29e-26 102
MS.gene25138.t1 MTR_4g017050 33.810 210 126 7 1 200 7 213 2.00e-26 101
MS.gene25138.t1 MTR_5g046410 33.810 210 126 7 1 200 7 213 2.34e-26 101
MS.gene25138.t1 MTR_5g046430 33.810 210 126 7 1 200 7 213 2.34e-26 101
MS.gene25138.t1 MTR_4g017030 33.810 210 126 7 1 200 7 213 2.34e-26 101
MS.gene25138.t1 MTR_8g007300 32.821 195 125 4 2 191 11 204 1.16e-25 99.4
MS.gene25138.t1 MTR_2g030895 35.465 172 104 4 39 203 7 178 4.52e-25 96.7
MS.gene25138.t1 MTR_6g005380 36.571 175 101 6 1 167 6 178 4.58e-24 95.1
MS.gene25138.t1 MTR_2g030865 34.884 172 105 4 39 203 7 178 1.27e-23 92.8
MS.gene25138.t1 MTR_3g096330 36.601 153 78 3 29 167 584 731 6.39e-22 93.6
MS.gene25138.t1 MTR_6g005350 33.333 189 117 6 22 203 28 214 3.16e-21 87.8
MS.gene25138.t1 MTR_2g086620 32.353 170 109 3 38 203 37 204 5.31e-20 84.3
MS.gene25138.t1 MTR_2g086630 31.765 170 110 3 38 203 37 204 1.39e-19 83.2
MS.gene25138.t1 MTR_2g086640 34.074 135 88 1 70 203 69 203 7.35e-19 81.3
MS.gene25138.t1 MTR_4g052780 33.136 169 110 2 38 203 37 205 9.04e-19 80.9
MS.gene25138.t1 MTR_4g052770 33.136 169 110 2 38 203 37 205 1.16e-18 80.9
MS.gene25138.t1 MTR_2g031300 32.500 160 82 4 39 192 7 146 1.70e-16 73.6
MS.gene25138.t1 MTR_2g096110 30.469 128 73 2 41 167 7 119 1.27e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25138.t1 AT1G72610 53.368 193 88 1 17 207 16 208 8.20e-72 217
MS.gene25138.t1 AT5G20630 50.000 208 101 2 1 206 4 210 4.26e-68 207
MS.gene25138.t1 AT1G18980 37.073 205 125 2 3 203 14 218 1.60e-42 142
MS.gene25138.t1 AT1G18970 35.122 205 129 2 1 203 16 218 4.23e-39 134
MS.gene25138.t1 AT3G04200 40.838 191 105 4 22 204 33 223 1.28e-38 133
MS.gene25138.t1 AT3G05930 37.363 182 110 3 2 179 8 189 1.21e-34 122
MS.gene25138.t1 AT5G26700 37.438 203 117 5 4 200 8 206 1.23e-34 122
MS.gene25138.t1 AT1G09560 36.022 186 115 2 22 203 30 215 4.55e-33 118
MS.gene25138.t1 AT5G38960 37.306 193 110 7 22 204 32 223 5.48e-32 115
MS.gene25138.t1 AT1G02335 35.443 158 100 1 22 177 30 187 2.06e-31 114
MS.gene25138.t1 AT3G10080 34.715 193 114 5 20 203 30 219 2.11e-31 114
MS.gene25138.t1 AT3G04150 34.112 214 133 4 2 207 12 225 3.51e-31 114
MS.gene25138.t1 AT3G10080 34.715 193 114 5 20 203 101 290 1.18e-30 114
MS.gene25138.t1 AT3G62020 30.918 207 138 3 3 204 10 216 3.75e-30 111
MS.gene25138.t1 AT3G04150 32.159 227 133 4 2 207 12 238 4.34e-30 111
MS.gene25138.t1 AT3G05950 37.895 190 110 4 22 203 32 221 5.17e-30 110
MS.gene25138.t1 AT5G61750 33.523 176 101 3 2 167 5 174 1.22e-29 109
MS.gene25138.t1 AT5G39110 34.579 214 127 5 3 203 7 220 2.19e-29 109
MS.gene25138.t1 AT1G74820 32.558 172 110 1 1 166 15 186 2.83e-29 108
MS.gene25138.t1 AT5G38910 33.971 209 128 5 4 203 12 219 1.46e-28 107
MS.gene25138.t1 AT3G04190 36.649 191 111 6 22 203 32 221 3.81e-28 105
MS.gene25138.t1 AT5G38940 34.737 190 116 4 22 203 32 221 6.30e-28 105
MS.gene25138.t1 AT5G39160 34.896 192 111 5 22 204 30 216 7.16e-28 105
MS.gene25138.t1 AT5G38940 34.737 190 116 4 22 203 56 245 9.59e-28 105
MS.gene25138.t1 AT5G39160 34.896 192 115 5 22 204 30 220 1.60e-27 104
MS.gene25138.t1 AT5G39190 34.896 192 115 5 22 204 30 220 1.76e-27 104
MS.gene25138.t1 AT5G38930 34.737 190 116 5 22 203 32 221 3.10e-27 103
MS.gene25138.t1 AT5G39120 32.243 214 133 6 1 203 7 219 5.04e-27 103
MS.gene25138.t1 AT5G39130 33.488 215 129 6 3 204 7 220 2.48e-26 101
MS.gene25138.t1 AT3G62020 31.609 174 114 3 35 203 13 186 3.19e-26 100
MS.gene25138.t1 AT5G39150 30.986 213 136 5 1 203 8 219 4.49e-26 100
MS.gene25138.t1 AT5G39180 30.986 213 136 5 1 203 8 219 6.58e-26 100
MS.gene25138.t1 AT3G04180 35.204 196 107 7 22 203 32 221 1.78e-25 99.0
MS.gene25138.t1 AT4G14630 34.762 210 125 7 5 203 14 222 2.51e-25 98.6
MS.gene25138.t1 AT3G04170 36.082 194 105 8 22 203 32 218 2.92e-24 95.9
MS.gene25138.t1 AT1G10460 34.568 162 99 4 22 176 25 186 5.31e-24 95.1
MS.gene25138.t1 AT5G39160 35.065 154 94 3 56 204 46 198 2.84e-23 92.8
MS.gene25138.t1 AT5G39190 35.065 154 94 3 56 204 46 198 3.12e-23 92.4

Find 27 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAATTTCCTGCTGTTAA+TGG 0.167755 8.4:-72978130 MS.gene25138:CDS
GTTTACTCCAACTTCAATTC+AGG 0.252771 8.4:-72978202 MS.gene25138:CDS
ATTCTTGATCTTGCTTTGTT+TGG 0.254800 8.4:-72977839 MS.gene25138:CDS
ACTTTCTAAAGGTCAAATTT+TGG 0.284643 8.4:-72977957 MS.gene25138:CDS
TTTCCTGCTGTTAATGGTCT+TGG 0.310444 8.4:-72978124 MS.gene25138:CDS
TCTTCTCCACAAACCCCTTC+AGG 0.334680 8.4:-72978262 MS.gene25138:CDS
TGGTTTATCAGCAGCAAGAT+TGG 0.394978 8.4:-72978104 MS.gene25138:CDS
TAATACAGAAGCATTGGTTA+TGG 0.446790 8.4:+72978306 None:intergenic
GGGTAGCCTGAAGGGGTTTG+TGG 0.451614 8.4:+72978256 None:intergenic
GCATTCACATCGCGGCGCTT+CGG 0.455830 8.4:-72978053 MS.gene25138:CDS
GGAAATTGATTAACAGATGC+AGG 0.477175 8.4:+72978142 None:intergenic
TTTATCAAGACACTTTCTAA+AGG 0.499284 8.4:-72977968 MS.gene25138:CDS
CGCTTCGGAGTTAATGATTG+TGG 0.511937 8.4:-72978038 MS.gene25138:CDS
GAAGTCTAATACAGAAGCAT+TGG 0.518908 8.4:+72978300 None:intergenic
AGTGTTTCCTGAATTGAAGT+TGG 0.520987 8.4:+72978195 None:intergenic
GCAAGATTGGACTTCGACGT+TGG 0.522667 8.4:-72978091 MS.gene25138:CDS
GTACAAGGTCGTATAACTGC+TGG 0.537303 8.4:-72978016 MS.gene25138:CDS
AGATCAAGAATTTGTGAACC+AGG 0.540464 8.4:+72977851 None:intergenic
TTTGCATGGGTAGCCTGAAG+GGG 0.553451 8.4:+72978249 None:intergenic
AGATTGGACTTCGACGTTGG+AGG 0.578726 8.4:-72978088 MS.gene25138:CDS
GCGCCGCGATGTGAATGCAT+TGG 0.582043 8.4:+72978058 None:intergenic
AAACCAAGACCATTAACAGC+AGG 0.584052 8.4:+72978121 None:intergenic
GAGTTAATGATTGTGGTACA+AGG 0.597384 8.4:-72978031 MS.gene25138:CDS
GATCAAGAATTTGTGAACCA+GGG 0.620980 8.4:+72977852 None:intergenic
AATGTGAGAGAAGAGTTAAG+AGG 0.621704 8.4:+72978329 None:intergenic
TGAAAATGCAACAAACCTTG+TGG 0.665243 8.4:+72977929 None:intergenic
ATACCAATGCATTCACATCG+CGG 0.682883 8.4:-72978061 MS.gene25138:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTATCAAGACACTTTCTAA+AGG - chr8.4:72978123-72978142 MS.gene25138:CDS 25.0%
!! ATTTTCAATATAATGCTGGA+AGG - chr8.4:72978178-72978197 MS.gene25138:CDS 25.0%
!! TTGCATTTTCAATATAATGC+TGG - chr8.4:72978174-72978193 MS.gene25138:CDS 25.0%
!!! AAAAAGCTTAAGGCTATTTT+TGG - chr8.4:72978333-72978352 MS.gene25138:CDS 25.0%
!!! ACTTTCTAAAGGTCAAATTT+TGG - chr8.4:72978134-72978153 MS.gene25138:CDS 25.0%
!!! ATTGTTACTGATTTTTTGCA+TGG + chr8.4:72977859-72977878 None:intergenic 25.0%
!!! TTGTTACTGATTTTTTGCAT+GGG + chr8.4:72977858-72977877 None:intergenic 25.0%
AATCAATTTCCTGCTGTTAA+TGG - chr8.4:72977961-72977980 MS.gene25138:CDS 30.0%
TGCTCAAGTTAAAAAGCTTA+AGG - chr8.4:72978323-72978342 MS.gene25138:CDS 30.0%
! ATTCTTGATCTTGCTTTGTT+TGG - chr8.4:72978252-72978271 MS.gene25138:CDS 30.0%
!! TAATACAGAAGCATTGGTTA+TGG + chr8.4:72977788-72977807 None:intergenic 30.0%
!!! TTAAGCTTTTTAACTTGAGC+AGG + chr8.4:72978324-72978343 None:intergenic 30.0%
AATGTGAGAGAAGAGTTAAG+AGG + chr8.4:72977765-72977784 None:intergenic 35.0%
AGATCAAGAATTTGTGAACC+AGG + chr8.4:72978243-72978262 None:intergenic 35.0%
AGTGTTTCCTGAATTGAAGT+TGG + chr8.4:72977899-72977918 None:intergenic 35.0%
GAGTTAATGATTGTGGTACA+AGG - chr8.4:72978060-72978079 MS.gene25138:CDS 35.0%
GATCAAGAATTTGTGAACCA+GGG + chr8.4:72978242-72978261 None:intergenic 35.0%
GGAAATTGATTAACAGATGC+AGG + chr8.4:72977952-72977971 None:intergenic 35.0%
GTTTACTCCAACTTCAATTC+AGG - chr8.4:72977889-72977908 MS.gene25138:CDS 35.0%
TGAAAATGCAACAAACCTTG+TGG + chr8.4:72978165-72978184 None:intergenic 35.0%
! GAAGTCTAATACAGAAGCAT+TGG + chr8.4:72977794-72977813 None:intergenic 35.0%
!!! AAGCTTAAGGCTATTTTTGG+TGG - chr8.4:72978336-72978355 MS.gene25138:CDS 35.0%
!!! AGCTTAAGGCTATTTTTGGT+GGG - chr8.4:72978337-72978356 MS.gene25138:CDS 35.0%
AAACCAAGACCATTAACAGC+AGG + chr8.4:72977973-72977992 None:intergenic 40.0%
ATACCAATGCATTCACATCG+CGG - chr8.4:72978030-72978049 MS.gene25138:CDS 40.0%
TGGTTTATCAGCAGCAAGAT+TGG - chr8.4:72977987-72978006 MS.gene25138:CDS 40.0%
TTTCCTGCTGTTAATGGTCT+TGG - chr8.4:72977967-72977986 MS.gene25138:CDS 40.0%
! ACTTTTAGTAGTGAGAACCC+TGG - chr8.4:72978222-72978241 MS.gene25138:CDS 40.0%
!!! CAAATTTTGGCTCTACCACA+AGG - chr8.4:72978147-72978166 MS.gene25138:CDS 40.0%
CGCTTCGGAGTTAATGATTG+TGG - chr8.4:72978053-72978072 MS.gene25138:CDS 45.0%
GTACAAGGTCGTATAACTGC+TGG - chr8.4:72978075-72978094 MS.gene25138:CDS 45.0%
! TTTTGCATGGGTAGCCTGAA+GGG + chr8.4:72977846-72977865 None:intergenic 45.0%
! TTTTTGCATGGGTAGCCTGA+AGG + chr8.4:72977847-72977866 None:intergenic 45.0%
!!! AAGGCTATTTTTGGTGGGAG+TGG - chr8.4:72978342-72978361 MS.gene25138:CDS 45.0%
AGATTGGACTTCGACGTTGG+AGG - chr8.4:72978003-72978022 MS.gene25138:CDS 50.0%
GCAAGATTGGACTTCGACGT+TGG - chr8.4:72978000-72978019 MS.gene25138:CDS 50.0%
TCTTCTCCACAAACCCCTTC+AGG - chr8.4:72977829-72977848 MS.gene25138:CDS 50.0%
TTTGCATGGGTAGCCTGAAG+GGG + chr8.4:72977845-72977864 None:intergenic 50.0%
GCATTCACATCGCGGCGCTT+CGG - chr8.4:72978038-72978057 MS.gene25138:CDS 60.0%
GCGCCGCGATGTGAATGCAT+TGG + chr8.4:72978036-72978055 None:intergenic 60.0%
GGGTAGCCTGAAGGGGTTTG+TGG + chr8.4:72977838-72977857 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 72977745 72978368 72977745 ID=MS.gene25138
chr8.4 mRNA 72977745 72978368 72977745 ID=MS.gene25138.t1;Parent=MS.gene25138
chr8.4 exon 72977745 72978368 72977745 ID=MS.gene25138.t1.exon1;Parent=MS.gene25138.t1
chr8.4 CDS 72977745 72978368 72977745 ID=cds.MS.gene25138.t1;Parent=MS.gene25138.t1
Gene Sequence

>MS.gene25138

ATGATTTTCATTCTTTTCCTCTTAACTCTTCTCTCACATTCCATAACCAATGCTTCTGTATTAGACTTCTGTGTAGCAGATTTATCTTCTCCACAAACCCCTTCAGGCTACCCATGCAAAAAATCAGTAACAATCAATGACTTTGTTTACTCCAACTTCAATTCAGGAAACACTTCAGCAAATTTTTTCAAATTTTCAATAACTCCTGCATCTGTTAATCAATTTCCTGCTGTTAATGGTCTTGGTTTATCAGCAGCAAGATTGGACTTCGACGTTGGAGGTATAATACCAATGCATTCACATCGCGGCGCTTCGGAGTTAATGATTGTGGTACAAGGTCGTATAACTGCTGGTTTTATTTCTTCTGATAATACTGTTTTTATCAAGACACTTTCTAAAGGTCAAATTTTGGCTCTACCACAAGGTTTGTTGCATTTTCAATATAATGCTGGAAGGAATAAAGCTTCTGCTTATCTTACTTTTAGTAGTGAGAACCCTGGTTCACAAATTCTTGATCTTGCTTTGTTTGGAAATAATTTGAGTTCTGCTAATGTGATAAAGAGTACTTTGCTTGATCCTGCTCAAGTTAAAAAGCTTAAGGCTATTTTTGGTGGGAGTGGCTAG

Protein sequence

>MS.gene25138.t1

MIFILFLLTLLSHSITNASVLDFCVADLSSPQTPSGYPCKKSVTINDFVYSNFNSGNTSANFFKFSITPASVNQFPAVNGLGLSAARLDFDVGGIIPMHSHRGASELMIVVQGRITAGFISSDNTVFIKTLSKGQILALPQGLLHFQYNAGRNKASAYLTFSSENPGSQILDLALFGNNLSSANVIKSTLLDPAQVKKLKAIFGGSG