AlfalfaGEDB | MS.gene25682

M. sativa cultivar XinJiangDaYe / MS.gene25682

KEGG: K11816
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004497 GO:0004499 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0016709 GO:0036094 GO:0043167 GO:0043168 GO:0048037 GO:0050660 GO:0050661 GO:0050662 GO:0055114 GO:0097159 GO:1901265 GO:1901363
PFAM: PF00743.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25682.t1	XP_013450336.1	95.8	168	6	1	1	167	1	168	1.00E-86	329.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25682.t1	Q9LKC0	52.7	167	74	3	7	168	26	192	7.1e-44	178.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25682.t1	A0A072U3T1	95.8	168	6	1	1	167	1	168	7.4e-87	329.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050128	MS.gene25682	0.803407	3.54E-49	-1.69E-46
MS.gene050971	MS.gene25682	0.811263	7.64E-51	-1.69E-46
MS.gene051521	MS.gene25682	0.823301	1.49E-53	-1.69E-46
MS.gene052077	MS.gene25682	0.800449	1.43E-48	-1.69E-46
MS.gene052188	MS.gene25682	0.808928	2.43E-50	-1.69E-46
MS.gene05316	MS.gene25682	0.86721	1.62E-65	-1.69E-46
MS.gene053494	MS.gene25682	0.815913	7.24E-52	-1.69E-46
MS.gene054183	MS.gene25682	0.813321	2.72E-51	-1.69E-46
MS.gene054349	MS.gene25682	0.829032	6.46E-55	-1.69E-46
MS.gene054556	MS.gene25682	0.801567	8.47E-49	-1.69E-46
MS.gene056723	MS.gene25682	0.805497	1.30E-49	-1.69E-46
MS.gene057396	MS.gene25682	0.839241	1.78E-57	-1.69E-46
MS.gene057449	MS.gene25682	0.8006	1.34E-48	-1.69E-46
MS.gene057537	MS.gene25682	0.809208	2.12E-50	-1.69E-46
MS.gene057609	MS.gene25682	0.840291	9.50E-58	-1.69E-46
MS.gene057610	MS.gene25682	0.860234	2.38E-63	-1.69E-46
MS.gene058075	MS.gene25682	0.828457	8.90E-55	-1.69E-46
MS.gene058435	MS.gene25682	0.800946	1.14E-48	-1.69E-46
MS.gene05872	MS.gene25682	0.816562	5.19E-52	-1.69E-46
MS.gene058949	MS.gene25682	0.846467	2.13E-59	-1.69E-46
MS.gene059294	MS.gene25682	0.80374	3.02E-49	-1.69E-46
MS.gene059420	MS.gene25682	0.801006	1.10E-48	-1.69E-46
MS.gene059807	MS.gene25682	0.849286	3.56E-60	-1.69E-46
MS.gene059876	MS.gene25682	0.824625	7.29E-54	-1.69E-46
MS.gene060495	MS.gene25682	0.837441	5.19E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25682.t1	MTR_7g107710	95.833	168	6	1	1	167	1	168	1.11e-115	333
MS.gene25682.t1	MTR_7g107690	67.901	162	52	0	6	167	8	169	1.00e-80	244
MS.gene25682.t1	MTR_1g046230	55.625	160	69	1	7	164	25	184	2.70e-56	182
MS.gene25682.t1	MTR_7g099330	55.280	161	66	2	7	167	25	179	2.86e-55	179
MS.gene25682.t1	MTR_8g432640	51.220	164	80	0	1	164	1	164	3.95e-55	178
MS.gene25682.t1	MTR_1g069275	55.975	159	67	2	7	164	26	182	7.51e-55	178
MS.gene25682.t1	MTR_5g033260	49.390	164	83	0	1	164	1	164	1.12e-52	172
MS.gene25682.t1	MTR_3g109520	50.000	158	79	0	7	164	40	197	3.92e-51	169
MS.gene25682.t1	MTR_4g051642	48.447	161	83	0	3	163	4	164	4.21e-51	168
MS.gene25682.t1	MTR_3g088955	47.205	161	85	0	3	163	4	164	1.06e-50	167
MS.gene25682.t1	MTR_3g088945	47.205	161	85	0	3	163	4	164	2.52e-50	166
MS.gene25682.t1	MTR_3g088925	47.205	161	85	0	3	163	4	164	2.67e-50	166
MS.gene25682.t1	MTR_1g008380	51.266	158	76	1	7	164	43	199	7.69e-50	166
MS.gene25682.t1	MTR_6g086870	49.689	161	78	2	7	164	21	181	1.64e-49	164
MS.gene25682.t1	MTR_1g011630	47.468	158	79	2	7	164	17	170	1.14e-47	159
MS.gene25682.t1	MTR_3g096030	40.854	164	73	2	1	164	1	140	1.26e-38	129
MS.gene25682.t1	MTR_7g099160	48.387	93	42	2	75	167	214	300	4.18e-18	80.9
MS.gene25682.t1	MTR_1g041605	75.000	40	10	0	110	149	29	68	2.40e-15	67.4
MS.gene25682.t1	MTR_2g044140	28.342	187	100	6	8	165	16	197	3.90e-13	66.6
MS.gene25682.t1	MTR_2g450310	27.128	188	109	6	3	163	2	188	8.16e-12	62.8
MS.gene25682.t1	MTR_2g450510	26.064	188	111	5	3	163	2	188	2.77e-11	61.2
MS.gene25682.t1	MTR_2g043850	25.946	185	107	5	8	165	16	197	3.66e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25682.t1	AT4G28720	53.125	160	73	2	7	164	26	185	3.94e-55	179
MS.gene25682.t1	AT4G13260	50.000	158	79	0	7	164	29	186	2.14e-54	174
MS.gene25682.t1	AT5G43890	53.750	160	72	2	7	164	26	185	5.12e-54	176
MS.gene25682.t1	AT1G04180	52.830	159	72	2	7	164	26	182	7.92e-54	176
MS.gene25682.t1	AT1G21430	51.875	160	74	2	6	164	9	166	9.48e-54	175
MS.gene25682.t1	AT1G48910	47.273	165	87	0	3	167	2	166	1.82e-53	174
MS.gene25682.t1	AT1G04610	54.037	161	71	2	7	164	38	198	2.59e-53	175
MS.gene25682.t1	AT4G13260	50.000	158	79	0	7	164	29	186	3.92e-53	174
MS.gene25682.t1	AT2G33230	54.037	161	71	2	7	164	33	193	3.07e-51	169
MS.gene25682.t1	AT5G11320	49.367	158	74	1	7	164	18	169	1.32e-50	166
MS.gene25682.t1	AT5G11320	49.367	158	74	1	7	164	18	169	4.44e-50	166
MS.gene25682.t1	AT5G25620	51.250	160	74	2	7	164	33	190	1.81e-49	164
MS.gene25682.t1	AT5G25620	51.250	160	74	2	7	164	33	190	2.69e-49	164
MS.gene25682.t1	AT4G32540	45.912	159	80	2	7	164	22	175	1.09e-43	149
MS.gene25682.t1	AT4G32540	45.912	159	80	2	7	164	22	175	1.16e-43	149
MS.gene25682.t1	AT5G45180	28.866	194	103	8	1	165	7	194	5.04e-13	66.2
MS.gene25682.t1	AT4G13260	38.158	76	47	0	89	164	8	83	6.89e-13	65.5
MS.gene25682.t1	AT1G65860	25.668	187	109	4	6	164	13	197	1.52e-11	62.0
MS.gene25682.t1	AT5G07800	25.907	193	109	4	6	164	16	208	1.79e-11	61.6

Find 0 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAACAGTTCATAGAATATTT+AGG	-	51694:1194-1213	MS.gene25682:CDS	20.0%
!!!	ATATTCTATGAACTGTTTTT+TGG	+	51694:1192-1211	MS.gene25682:intergenic	20.0%
!!!	TATTCTATGAACTGTTTTTT+GGG	+	51694:1191-1210	MS.gene25682:intergenic	20.0%
!	ATTCAACTAGGTACAAAAAA+TGG	-	51694:1441-1460	MS.gene25682:CDS	25.0%
!!	AAAACTGTTTTGCTTCTTTA+TGG	-	51694:1069-1088	MS.gene25682:CDS	25.0%
	ACTAGGTACAAAAAATGGAA+AGG	-	51694:1446-1465	MS.gene25682:CDS	30.0%
!!	ATGTTGATGATGATGAGAAA+TGG	-	51694:1279-1298	MS.gene25682:CDS	30.0%
	AAAGCTGAGAATAAGAGTTC+TGG	-	51694:1308-1327	MS.gene25682:CDS	35.0%
	CAACAGCTCTGTTGTATATA+GGG	+	51694:1247-1266	MS.gene25682:intergenic	35.0%
	TCAACAGCTCTGTTGTATAT+AGG	+	51694:1248-1267	MS.gene25682:intergenic	35.0%
	TCTCTTTCGAGAATGATGAA+AGG	+	51694:1050-1069	MS.gene25682:intergenic	35.0%
	TTCGAGAATGATGAAAGGTA+TGG	+	51694:1045-1064	MS.gene25682:intergenic	35.0%
!	TGATGATGATGAGAAATGGA+GGG	-	51694:1283-1302	MS.gene25682:CDS	35.0%
!!	TTGATGATGATGAGAAATGG+AGG	-	51694:1282-1301	MS.gene25682:CDS	35.0%
!!!	TGTTTTTTGGGTACATAGTG+AGG	+	51694:1179-1198	MS.gene25682:intergenic	35.0%
	AAGGGTTGGAGAGTTTCAAA+GGG	-	51694:1408-1427	MS.gene25682:CDS	40.0%
	ACATAGTGAGGATAAGAAGG+TGG	+	51694:1167-1186	MS.gene25682:intergenic	40.0%
	AGATTCTTGGTTGTTGCTAG+TGG	-	51694:1353-1372	MS.gene25682:CDS	40.0%
	ATGAAGATGAACACGATCGT+AGG	+	51694:1105-1124	MS.gene25682:intergenic	40.0%
	GGAAAGTGATGCATTCAACT+AGG	-	51694:1429-1448	MS.gene25682:CDS	40.0%
	GGTACATAGTGAGGATAAGA+AGG	+	51694:1170-1189	MS.gene25682:intergenic	40.0%
	TGAGGATAAGAAGGTGGAAA+AGG	+	51694:1161-1180	MS.gene25682:intergenic	40.0%
!	GGAGTATTCTGGAAGATTCT+TGG	-	51694:1340-1359	MS.gene25682:CDS	40.0%
!	TAAGAGTTCTGGTGAAGTTG+AGG	-	51694:1319-1338	MS.gene25682:CDS	40.0%
!!	GAAGCACCGGTGATAATAAT+CGG	-	51694:972-991	MS.gene25682:CDS	40.0%
	ATAATCGGAGCTGGAACATC+CGG	-	51694:987-1006	MS.gene25682:CDS	45.0%
	CCAGCTCCGATTATTATCAC+CGG	+	51694:981-1000	MS.gene25682:intergenic	45.0%
	GAAGGGTTGGAGAGTTTCAA+AGG	-	51694:1407-1426	MS.gene25682:CDS	45.0%
	GAAGGTGGAAAAGGAAAGTG+TGG	+	51694:1152-1171	MS.gene25682:intergenic	45.0%
!	TTTTGTTAAGCAAGCTGCCG+TGG	+	51694:1018-1037	MS.gene25682:intergenic	45.0%
!!	GGTGAAGTTGAGGAGTATTC+TGG	-	51694:1329-1348	MS.gene25682:CDS	45.0%
	CCGGTGATAATAATCGGAGC+TGG	-	51694:978-997	MS.gene25682:CDS	50.0%
	CTCGTGTACCTGAGGTTGAA+GGG	-	51694:1390-1409	MS.gene25682:CDS	50.0%
	TGTACCTGAGGTTGAAGGGT+TGG	-	51694:1394-1413	MS.gene25682:CDS	50.0%
	AGCTGAACCTCGTGTACCTG+AGG	-	51694:1382-1401	MS.gene25682:CDS	55.0%
	CCTCGTGTACCTGAGGTTGA+AGG	-	51694:1389-1408	MS.gene25682:CDS	55.0%
	CCTTCAACCTCAGGTACACG+AGG	+	51694:1392-1411	MS.gene25682:intergenic	55.0%
	CTCTCCAACCCTTCAACCTC+AGG	+	51694:1401-1420	MS.gene25682:intergenic	55.0%
	TTAAGCAAGCTGCCGTGGCT+AGG	+	51694:1013-1032	MS.gene25682:intergenic	55.0%
	TGGAACATCCGGCCTAGCCA+CGG	-	51694:998-1017	MS.gene25682:CDS	60.0%
	GCAAGCTGCCGTGGCTAGGC+CGG	+	51694:1009-1028	MS.gene25682:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
51694	gene	966	1475	966	ID=MS.gene25682
51694	mRNA	966	1475	966	ID=MS.gene25682.t1;Parent=MS.gene25682
51694	exon	966	1475	966	ID=MS.gene25682.t1.exon1;Parent=MS.gene25682.t1
51694	CDS	966	1475	966	ID=cds.MS.gene25682.t1;Parent=MS.gene25682.t1

Gene Sequence

>MS.gene25682

ATGCATGAAGCACCGGTGATAATAATCGGAGCTGGAACATCCGGCCTAGCCACGGCAGCTTGCTTAACAAAACAATCCATACCTTTCATCATTCTCGAAAGAGAAAACTGTTTTGCTTCTTTATGGCAAAACTACACCTACGATCGTGTTCATCTTCATCTAAGAAAACAACTTTGTGAGTTACCACACTTTCCTTTTCCACCTTCTTATCCTCACTATGTACCCAAAAAACAGTTCATAGAATATTTAGGTAACTATGTCAACAACTTCAACATCAACCCTATATACAACAGAGCTGTTGAGTTAGCAGAGTATGTTGATGATGATGAGAAATGGAGGGTTAAAGCTGAGAATAAGAGTTCTGGTGAAGTTGAGGAGTATTCTGGAAGATTCTTGGTTGTTGCTAGTGGTGAAACAGCTGAACCTCGTGTACCTGAGGTTGAAGGGTTGGAGAGTTTCAAAGGGAAAGTGATGCATTCAACTAGGTACAAAAAATGGAAAGGAGTTTAA

Protein sequence

>MS.gene25682.t1

MHEAPVIIIGAGTSGLATAACLTKQSIPFIILERENCFASLWQNYTYDRVHLHLRKQLCELPHFPFPPSYPHYVPKKQFIEYLGNYVNNFNINPIYNRAVELAEYVDDDEKWRVKAENKSSGEVEEYSGRFLVVASGETAEPRVPEVEGLESFKGKVMHSTRYKKWKGV