Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25968.t1 | XP_003601259.1 | 99.2 | 864 | 6 | 1 | 1 | 863 | 1 | 864 | 0.00E+00 | 1699.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25968.t1 | Q8L5Y9 | 85.7 | 844 | 115 | 5 | 25 | 863 | 29 | 871 | 0.0e+00 | 1441.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25968.t1 | G7J5F4 | 99.2 | 864 | 6 | 1 | 1 | 863 | 1 | 864 | 0.0e+00 | 1699.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049264 | MS.gene25968 | 0.81099 | 8.76E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene25968 | 0.822566 | 2.21E-53 | -1.69E-46 |
| MS.gene052389 | MS.gene25968 | 0.809115 | 2.22E-50 | -1.69E-46 |
| MS.gene053147 | MS.gene25968 | 0.820082 | 8.28E-53 | -1.69E-46 |
| MS.gene054468 | MS.gene25968 | 0.835176 | 1.95E-56 | -1.69E-46 |
| MS.gene054581 | MS.gene25968 | 0.809996 | 1.44E-50 | -1.69E-46 |
| MS.gene054826 | MS.gene25968 | 0.894759 | 1.82E-75 | -1.69E-46 |
| MS.gene05595 | MS.gene25968 | 0.823724 | 1.19E-53 | -1.69E-46 |
| MS.gene057929 | MS.gene25968 | 0.808645 | 2.80E-50 | -1.69E-46 |
| MS.gene058650 | MS.gene25968 | 0.842327 | 2.77E-58 | -1.69E-46 |
| MS.gene058651 | MS.gene25968 | 0.854078 | 1.56E-61 | -1.69E-46 |
| MS.gene05872 | MS.gene25968 | 0.826672 | 2.39E-54 | -1.69E-46 |
| MS.gene060373 | MS.gene25968 | 0.815899 | 7.30E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene25968 | 0.800602 | 1.33E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25968.t1 | MTR_3g077740 | 99.421 | 864 | 4 | 1 | 1 | 863 | 1 | 864 | 0.0 | 1762 |
| MS.gene25968.t1 | MTR_8g064850 | 61.463 | 410 | 148 | 3 | 44 | 452 | 1 | 401 | 1.10e-172 | 506 |
| MS.gene25968.t1 | MTR_8g064850 | 62.751 | 349 | 120 | 3 | 44 | 391 | 1 | 340 | 6.28e-149 | 442 |
| MS.gene25968.t1 | MTR_1g076720 | 35.833 | 360 | 187 | 9 | 518 | 863 | 11 | 340 | 3.56e-59 | 206 |
| MS.gene25968.t1 | MTR_6g060550 | 35.655 | 359 | 188 | 10 | 518 | 863 | 10 | 338 | 1.49e-58 | 204 |
| MS.gene25968.t1 | MTR_1g076730 | 35.792 | 366 | 192 | 9 | 512 | 863 | 158 | 494 | 2.46e-56 | 203 |
| MS.gene25968.t1 | MTR_6g060550 | 33.721 | 344 | 185 | 10 | 518 | 848 | 51 | 364 | 3.35e-47 | 173 |
| MS.gene25968.t1 | MTR_6g060550 | 33.724 | 341 | 183 | 10 | 518 | 845 | 10 | 320 | 4.14e-47 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25968.t1 | AT4G32180 | 85.664 | 844 | 115 | 5 | 25 | 863 | 29 | 871 | 0.0 | 1461 |
| MS.gene25968.t1 | AT4G32180 | 86.093 | 755 | 100 | 4 | 25 | 775 | 29 | 782 | 0.0 | 1333 |
| MS.gene25968.t1 | AT4G32180 | 86.093 | 755 | 100 | 4 | 25 | 775 | 29 | 782 | 0.0 | 1333 |
| MS.gene25968.t1 | AT1G60440 | 62.245 | 392 | 124 | 6 | 65 | 454 | 3 | 372 | 5.73e-147 | 439 |
| MS.gene25968.t1 | AT1G60440 | 70.790 | 291 | 83 | 2 | 165 | 454 | 6 | 295 | 1.28e-128 | 388 |
| MS.gene25968.t1 | AT1G60440 | 55.243 | 391 | 114 | 6 | 65 | 454 | 3 | 333 | 8.27e-120 | 367 |
| MS.gene25968.t1 | AT2G17320 | 37.719 | 342 | 181 | 9 | 524 | 863 | 21 | 332 | 9.38e-63 | 216 |
| MS.gene25968.t1 | AT2G17320 | 37.719 | 342 | 181 | 9 | 524 | 863 | 21 | 332 | 1.35e-62 | 215 |
| MS.gene25968.t1 | AT4G35360 | 38.671 | 331 | 171 | 9 | 535 | 863 | 38 | 338 | 1.63e-61 | 213 |
| MS.gene25968.t1 | AT4G35360 | 38.671 | 331 | 171 | 9 | 535 | 863 | 38 | 338 | 1.63e-61 | 213 |
| MS.gene25968.t1 | AT2G17340 | 37.609 | 343 | 174 | 9 | 523 | 863 | 34 | 338 | 1.94e-61 | 213 |
| MS.gene25968.t1 | AT4G35360 | 41.762 | 261 | 123 | 6 | 604 | 863 | 9 | 241 | 2.32e-55 | 192 |
Find 214 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAACCATCCTTACAATTT+TGG | 0.057026 | 3.3:-65943977 | None:intergenic |
| CAATTTGAACAAACTCTTTC+TGG | 0.085671 | 3.3:-65941577 | None:intergenic |
| TAGCTCTATTATCTCCTTTC+TGG | 0.143633 | 3.3:-65941967 | None:intergenic |
| TATAGCATTGTGACATTCTA+AGG | 0.194825 | 3.3:+65949113 | MS.gene25968:intron |
| TTTGCGACATGAAGGATTTC+TGG | 0.197061 | 3.3:+65942851 | MS.gene25968:CDS |
| TCCACTATTGCTTCAAGTTT+TGG | 0.201193 | 3.3:+65942212 | MS.gene25968:CDS |
| GTGCTATTTGCAGATAATTT+TGG | 0.221515 | 3.3:+65949392 | MS.gene25968:intron |
| GAAGGATTTCTGGGAGCTTT+AGG | 0.237803 | 3.3:+65942861 | MS.gene25968:CDS |
| GATAAGGGAACAAATCATTT+TGG | 0.246674 | 3.3:-65941601 | None:intergenic |
| TCTCATCTCGCTCTTGATAT+TGG | 0.262842 | 3.3:+65939169 | MS.gene25968:CDS |
| TTACTGTTTGTTGATAATTC+AGG | 0.264371 | 3.3:+65947420 | MS.gene25968:CDS |
| TCAGGTGCTGACATTGTTCT+AGG | 0.266086 | 3.3:+65947438 | MS.gene25968:CDS |
| AGGAGAAGCTTTCCTATTCT+TGG | 0.278927 | 3.3:+65940176 | MS.gene25968:CDS |
| TGGTGGTTTGGATTATTCTA+AGG | 0.289539 | 3.3:+65942015 | MS.gene25968:CDS |
| ACTCAAAGTTACCTTAATTA+TGG | 0.294989 | 3.3:-65940477 | None:intergenic |
| CCTCTGCTTTATACTTCTAT+AGG | 0.299214 | 3.3:-65946358 | None:intergenic |
| ATGTTGGTGGTGGTACTTAT+TGG | 0.301273 | 3.3:+65941804 | MS.gene25968:CDS |
| ATGCTTGTTGGGGACATTTA+TGG | 0.301696 | 3.3:+65941995 | MS.gene25968:CDS |
| TGTTGGTGGTGGTACTTATT+GGG | 0.303285 | 3.3:+65941805 | MS.gene25968:CDS |
| GCCAATCAGACGATGATAAA+AGG | 0.305514 | 3.3:+65940111 | MS.gene25968:CDS |
| TAAATACAGTATGGCTGATT+TGG | 0.309991 | 3.3:+65938946 | None:intergenic |
| CCCTTGATGGTTGTTGAGAA+TGG | 0.311987 | 3.3:+65949214 | MS.gene25968:CDS |
| AAAAGCATCACCCCTTCTTT+TGG | 0.318392 | 3.3:-65945490 | None:intergenic |
| GTTAGGCTTGGCCAATCTAT+TGG | 0.318807 | 3.3:+65946293 | MS.gene25968:CDS |
| TGTATCTTGCCACCAGTATA+TGG | 0.319902 | 3.3:-65942963 | None:intergenic |
| TGGGGACATTTATGGTGGTT+TGG | 0.324370 | 3.3:+65942003 | MS.gene25968:CDS |
| TTTCATTTGCTGTTCAATAC+TGG | 0.329419 | 3.3:+65942702 | MS.gene25968:CDS |
| GGGAAGTTTGAGAGGGTTAG+TGG | 0.335778 | 3.3:+65941776 | MS.gene25968:CDS |
| TGATCGAAATGCCATAATTA+AGG | 0.338567 | 3.3:+65940466 | MS.gene25968:CDS |
| CTTCTAGTTAATGTTGGTTC+CGG | 0.339897 | 3.3:+65941623 | MS.gene25968:CDS |
| AGGAGCAAATTTCTTGCTTA+AGG | 0.343687 | 3.3:+65940967 | MS.gene25968:CDS |
| CACTTTGTCAAATTTGAAAC+GGG | 0.349017 | 3.3:+65940209 | MS.gene25968:CDS |
| CTCTTCAAAGAGAGAATGTT+AGG | 0.349289 | 3.3:+65947360 | MS.gene25968:CDS |
| GCAGCCTCAGCTACAATATC+AGG | 0.349938 | 3.3:-65948151 | None:intergenic |
| TTCATCTTCGGTCCCCTTGA+TGG | 0.350155 | 3.3:+65949201 | MS.gene25968:CDS |
| CTTGAACTTGATCTATGTAT+TGG | 0.350994 | 3.3:-65939055 | None:intergenic |
| AAACATTGAAGGAGCGATTA+GGG | 0.351661 | 3.3:+65940138 | MS.gene25968:CDS |
| TAGGGCTTTCTAATGGTAAC+AGG | 0.355069 | 3.3:+65940156 | MS.gene25968:CDS |
| TTCCCTCATTTCCAATAGAT+TGG | 0.358243 | 3.3:-65946304 | None:intergenic |
| ACTCCTGTTGCTGGAACTAC+TGG | 0.372736 | 3.3:+65943833 | MS.gene25968:CDS |
| AGAGAGAGTGATATGTCTTC+AGG | 0.373424 | 3.3:-65942275 | None:intergenic |
| TTCTATAGGCATCAACAAAC+TGG | 0.374707 | 3.3:-65946344 | None:intergenic |
| GTGCCGATGACTCCTGTTGC+TGG | 0.376588 | 3.3:+65943824 | MS.gene25968:CDS |
| AAAACATTGAAGGAGCGATT+AGG | 0.380222 | 3.3:+65940137 | MS.gene25968:CDS |
| CCTTCTGCATACGGCAAGTT+AGG | 0.380263 | 3.3:+65946276 | MS.gene25968:CDS |
| GAGCTATCGACATGCTTGTT+GGG | 0.380873 | 3.3:+65941984 | MS.gene25968:CDS |
| CACCAAGTCTTTCTTTGAAA+AGG | 0.381637 | 3.3:-65940904 | None:intergenic |
| GTTTGTAGAACTTGATAGTA+TGG | 0.382267 | 3.3:+65946558 | MS.gene25968:CDS |
| TGCTGCAAAAGACGCCGATT+TGG | 0.388650 | 3.3:+65949288 | MS.gene25968:CDS |
| GTTTAAGTGTGATGCTTTGA+AGG | 0.388883 | 3.3:+65949455 | MS.gene25968:CDS |
| TGGTGGTACTTATTGGGGAC+TGG | 0.395806 | 3.3:+65941811 | MS.gene25968:CDS |
| ATTTGCTTACTCTAATTCAC+TGG | 0.397587 | 3.3:-65944452 | None:intergenic |
| CCGATGGGTGCTCCATATAC+TGG | 0.398412 | 3.3:+65942951 | MS.gene25968:CDS |
| TTTGGAAGTGTTTCCGTTGT+TGG | 0.401244 | 3.3:+65943949 | MS.gene25968:CDS |
| TGTTCTAGGGATGCTTCCTC+TGG | 0.402559 | 3.3:+65947452 | MS.gene25968:CDS |
| AAGACAGGTCAGCTCGGAAT+TGG | 0.403885 | 3.3:+65949261 | MS.gene25968:CDS |
| CCATGTGTGTGAAAGCTTCA+TGG | 0.404252 | 3.3:-65941550 | None:intergenic |
| TTTATTTGTCATAGGTTGAT+GGG | 0.404451 | 3.3:+65941750 | MS.gene25968:intron |
| CTTCCAAAGTTGGTACTAGA+TGG | 0.406132 | 3.3:-65943934 | None:intergenic |
| TCCAAAACTTGAAGCAATAG+TGG | 0.413680 | 3.3:-65942213 | None:intergenic |
| CCAACATTAACTAGAAGATA+AGG | 0.421746 | 3.3:-65941617 | None:intergenic |
| TTGTCATAGGTTGATGGGGA+TGG | 0.422373 | 3.3:+65941755 | MS.gene25968:intron |
| CATCGCCTGGGCTTCTCCAT+TGG | 0.428278 | 3.3:-65942826 | None:intergenic |
| TTGCGACATGAAGGATTTCT+GGG | 0.430140 | 3.3:+65942852 | MS.gene25968:CDS |
| CCTTATCTTCTAGTTAATGT+TGG | 0.434063 | 3.3:+65941617 | MS.gene25968:CDS |
| ACTACCTTGTCCACAAGATC+TGG | 0.434369 | 3.3:-65944586 | None:intergenic |
| TCTGCAAGATCGATTGTATT+GGG | 0.438407 | 3.3:-65944427 | None:intergenic |
| TGTCATAGGTTGATGGGGAT+GGG | 0.443864 | 3.3:+65941756 | MS.gene25968:intron |
| CTAAGGCGAGCTGCTGAAGC+TGG | 0.448195 | 3.3:+65949130 | MS.gene25968:CDS |
| ACTCACCTGACCGATGTTGT+AGG | 0.448748 | 3.3:-65942312 | None:intergenic |
| AGTCCAGTAGTTCCAGCAAC+AGG | 0.450308 | 3.3:-65943836 | None:intergenic |
| CCTTGATGGTTGTTGAGAAT+GGG | 0.452403 | 3.3:+65949215 | MS.gene25968:CDS |
| TTGCTGGAACTACTGGACTT+GGG | 0.454358 | 3.3:+65943840 | MS.gene25968:CDS |
| TCACTTTGTCAAATTTGAAA+CGG | 0.458203 | 3.3:+65940208 | MS.gene25968:CDS |
| ATCTGCAAGATCGATTGTAT+TGG | 0.462851 | 3.3:-65944428 | None:intergenic |
| AAGCAATAGTGGAAGCTGAT+AGG | 0.466489 | 3.3:-65942202 | None:intergenic |
| GAGCGATTAGGGCTTTCTAA+TGG | 0.468080 | 3.3:+65940149 | MS.gene25968:CDS |
| TTTGAAGTCCCTCTGTCAAA+AGG | 0.469850 | 3.3:+65943866 | MS.gene25968:CDS |
| CCCATTCTCAACAACCATCA+AGG | 0.471436 | 3.3:-65949215 | None:intergenic |
| ATCATCAGTTCCACCTTCAC+TGG | 0.473649 | 3.3:-65945466 | None:intergenic |
| AACAGTAAAGCTCTTCTATG+AGG | 0.474599 | 3.3:-65947405 | None:intergenic |
| CTCAAAAGAAAATTCATCTT+CGG | 0.475886 | 3.3:+65949189 | MS.gene25968:CDS |
| TGAAGCCTTCCACCAAGAAT+AGG | 0.477732 | 3.3:-65940188 | None:intergenic |
| AGACTGCTTACTCTAATTGA+AGG | 0.478438 | 3.3:+65947101 | MS.gene25968:CDS |
| GGTGGTACTTATTGGGGACT+GGG | 0.480437 | 3.3:+65941812 | MS.gene25968:CDS |
| AGATTGATTAGGCAACAAAA+TGG | 0.482598 | 3.3:-65939137 | None:intergenic |
| GTTGCTGGAACTACTGGACT+TGG | 0.485530 | 3.3:+65943839 | MS.gene25968:CDS |
| TAGGGATGCTTCCTCTGGCT+AGG | 0.486511 | 3.3:+65947457 | MS.gene25968:CDS |
| TGCTGGAACTACTGGACTTG+GGG | 0.488456 | 3.3:+65943841 | MS.gene25968:CDS |
| AATGAGGGAAGAGTGCTTGA+GGG | 0.489682 | 3.3:+65946317 | MS.gene25968:CDS |
| AGGTTCCAATGCAGATTTCG+TGG | 0.491662 | 3.3:+65943759 | MS.gene25968:intron |
| GAATGATCCTCCTACCAAAT+CGG | 0.494985 | 3.3:-65949302 | None:intergenic |
| TTGCTTGAGCTGAGCCAGAA+AGG | 0.495131 | 3.3:+65941953 | MS.gene25968:CDS |
| TAAAATGCGCAGACCTTGGC+GGG | 0.497032 | 3.3:+65947220 | MS.gene25968:CDS |
| TGCATACGGCAAGTTAGGCT+TGG | 0.498634 | 3.3:+65946281 | MS.gene25968:CDS |
| AGAGCTATCGACATGCTTGT+TGG | 0.498893 | 3.3:+65941983 | MS.gene25968:CDS |
| AGGGTTAGTGGGACAAATGT+TGG | 0.499572 | 3.3:+65941788 | MS.gene25968:CDS |
| CAACATTAACTAGAAGATAA+GGG | 0.500111 | 3.3:-65941616 | None:intergenic |
| TGTGTTGATCTCTATCATAA+AGG | 0.501529 | 3.3:+65947164 | MS.gene25968:CDS |
| TCTCCATCTAGTACCAACTT+TGG | 0.502815 | 3.3:+65943931 | MS.gene25968:CDS |
| CAATACATAGATCAAGTTCA+AGG | 0.505465 | 3.3:+65939056 | MS.gene25968:CDS |
| AACGGAAACACTTCCAAAGT+TGG | 0.505796 | 3.3:-65943944 | None:intergenic |
| AAAGAGAGAATGTTAGGGAC+CGG | 0.506027 | 3.3:+65947366 | MS.gene25968:CDS |
| ATGGGGATGGGAAGTTTGAG+AGG | 0.506268 | 3.3:+65941768 | MS.gene25968:CDS |
| AGGGATGCTTCCTCTGGCTA+GGG | 0.513859 | 3.3:+65947458 | MS.gene25968:CDS |
| TCCAATGCAGATTTCGTGGA+TGG | 0.514701 | 3.3:+65943763 | MS.gene25968:intron |
| GCAATAAAATGCGCAGACCT+TGG | 0.515821 | 3.3:+65947216 | MS.gene25968:CDS |
| GAACTGATGATGCCAAAAGA+AGG | 0.520146 | 3.3:+65945478 | MS.gene25968:CDS |
| TGGCCAATCTATTGGAAATG+AGG | 0.520415 | 3.3:+65946301 | MS.gene25968:CDS |
| CATCTCGCTCTTGATATTGG+AGG | 0.522421 | 3.3:+65939172 | MS.gene25968:CDS |
| ATGGAGGAGCCTTCTGCATA+CGG | 0.524279 | 3.3:+65946267 | MS.gene25968:CDS |
| GTCATTATTACCTTGACAAG+TGG | 0.524525 | 3.3:-65945530 | None:intergenic |
| TTCCGGTGTTAGTATGATCA+AGG | 0.525075 | 3.3:+65941640 | MS.gene25968:CDS |
| AACAACAACATGGCTCCTAC+AGG | 0.525280 | 3.3:+65939028 | MS.gene25968:CDS |
| ACATATCACTCTCTCTTCTA+CGG | 0.529289 | 3.3:+65942282 | MS.gene25968:CDS |
| CCATGAAGCTTTCACACACA+TGG | 0.530882 | 3.3:+65941550 | MS.gene25968:CDS |
| AAGTCTTTCTTTGAAAAGGT+CGG | 0.531063 | 3.3:-65940900 | None:intergenic |
| GGCCAATCTATTGGAAATGA+GGG | 0.531273 | 3.3:+65946302 | MS.gene25968:CDS |
| ACATCAAATGAATAGGTTGA+TGG | 0.531786 | 3.3:+65946248 | MS.gene25968:intron |
| GTTAGTTGAAAGGTTTCCGA+TGG | 0.532451 | 3.3:+65942935 | MS.gene25968:CDS |
| ACAGTAAAGCTCTTCTATGA+GGG | 0.532783 | 3.3:-65947404 | None:intergenic |
| TCAAGTTCTACAAACAAGTC+AGG | 0.533102 | 3.3:-65946550 | None:intergenic |
| TAAAAGGAAGAAAACATTGA+AGG | 0.533223 | 3.3:+65940127 | MS.gene25968:CDS |
| AGCAAATGAAAGAGTGTCCA+TGG | 0.533459 | 3.3:-65942690 | None:intergenic |
| AAGATACATGGCCCACCACT+TGG | 0.534164 | 3.3:+65942978 | MS.gene25968:CDS |
| GCAACAGGAGTCATCGGCAC+AGG | 0.535483 | 3.3:-65943821 | None:intergenic |
| GCTTTCCTATTCTTGGTGGA+AGG | 0.535678 | 3.3:+65940183 | MS.gene25968:CDS |
| TATACTGGTGGCAAGATACA+TGG | 0.537423 | 3.3:+65942966 | MS.gene25968:CDS |
| GTGATGAAGGCTACTGGAGG+TGG | 0.539097 | 3.3:+65940863 | MS.gene25968:intron |
| TGTGGATAATAGGGAAAATG+AGG | 0.541454 | 3.3:+65946510 | MS.gene25968:intron |
| TGGTGCATCAGTTAGTTGAA+AGG | 0.545222 | 3.3:+65942925 | MS.gene25968:CDS |
| TATTGTAGCTGAGGCTGCCA+AGG | 0.545712 | 3.3:+65948156 | MS.gene25968:CDS |
| ATAAAATGCGCAGACCTTGG+CGG | 0.549032 | 3.3:+65947219 | MS.gene25968:CDS |
| AAATGAGGGAAGAGTGCTTG+AGG | 0.549228 | 3.3:+65946316 | MS.gene25968:CDS |
| TTATTTGTCATAGGTTGATG+GGG | 0.552120 | 3.3:+65941751 | MS.gene25968:intron |
| AGAAGCTTTCCTATTCTTGG+TGG | 0.558179 | 3.3:+65940179 | MS.gene25968:CDS |
| GGAAGTTTGAGAGGGTTAGT+GGG | 0.558531 | 3.3:+65941777 | MS.gene25968:CDS |
| AAGAGAGAATGTTAGGGACC+GGG | 0.558973 | 3.3:+65947367 | MS.gene25968:CDS |
| GGCAGGATCTCTGATAAAGT+TGG | 0.559378 | 3.3:+65940067 | MS.gene25968:intron |
| TCATTATTACCTTGACAAGT+GGG | 0.560771 | 3.3:-65945529 | None:intergenic |
| TCATTCAAATCGCCAAGTGG+TGG | 0.560907 | 3.3:-65942990 | None:intergenic |
| TAGGTCCAATGGAGAAGCCC+AGG | 0.563858 | 3.3:+65942821 | MS.gene25968:intron |
| CAGGTGCTGACATTGTTCTA+GGG | 0.564205 | 3.3:+65947439 | MS.gene25968:CDS |
| ACCTGTCTTAAGTCTATACA+TGG | 0.565218 | 3.3:-65949247 | None:intergenic |
| GATTTCCTACAACATCGGTC+AGG | 0.567822 | 3.3:+65942307 | MS.gene25968:CDS |
| TCTTGGTATTGACGAATCTG+AGG | 0.572088 | 3.3:+65938997 | MS.gene25968:CDS |
| TGATTAGGCAACAAAATGGT+GGG | 0.574093 | 3.3:-65939133 | None:intergenic |
| GACAAATCATCAGATTGATT+AGG | 0.574243 | 3.3:-65939148 | None:intergenic |
| TTTGCTTACTCTAATTCACT+GGG | 0.574456 | 3.3:-65944451 | None:intergenic |
| CCATTCTCAACAACCATCAA+GGG | 0.574628 | 3.3:-65949214 | None:intergenic |
| CACCTTGATCATACTAACAC+CGG | 0.575151 | 3.3:-65941642 | None:intergenic |
| CAGGGGTCATGCTTATACCA+TGG | 0.575740 | 3.3:+65942673 | MS.gene25968:CDS |
| GCTTCCTGATATTGTAGCTG+AGG | 0.576697 | 3.3:+65948147 | MS.gene25968:CDS |
| GCAGTCACATCATTCAAAGC+AGG | 0.576932 | 3.3:-65948118 | None:intergenic |
| TTGATTAGGCAACAAAATGG+TGG | 0.577087 | 3.3:-65939134 | None:intergenic |
| AGGCGAGCTGCTGAAGCTGG+AGG | 0.578900 | 3.3:+65949133 | MS.gene25968:CDS |
| CAGGCTGTTGCCAGTGAAGG+TGG | 0.580036 | 3.3:+65945456 | MS.gene25968:intron |
| CCTAACTTGCCGTATGCAGA+AGG | 0.581095 | 3.3:-65946276 | None:intergenic |
| AGAGCACTTACCAGATCTTG+TGG | 0.584881 | 3.3:+65944576 | MS.gene25968:CDS |
| CATTCAAATCGCCAAGTGGT+GGG | 0.589143 | 3.3:-65942989 | None:intergenic |
| ACTTGGCGATTTGAATGAGA+AGG | 0.589246 | 3.3:+65942995 | MS.gene25968:CDS |
| GACTGTTAACAAAGTGCAAG+AGG | 0.589787 | 3.3:+65941837 | MS.gene25968:CDS |
| AGAACTTGATAGTATGGATG+AGG | 0.590772 | 3.3:+65946564 | MS.gene25968:CDS |
| TTAATTCCACCTACCCGCCA+AGG | 0.591741 | 3.3:-65947233 | None:intergenic |
| GTTCCAGCAACAGGAGTCAT+CGG | 0.593671 | 3.3:-65943827 | None:intergenic |
| CTTGTTGGGGACATTTATGG+TGG | 0.594785 | 3.3:+65941998 | MS.gene25968:CDS |
| TCTTCAAAGAGAGAATGTTA+GGG | 0.595349 | 3.3:+65947361 | MS.gene25968:CDS |
| CTTACCAGATCTTGTGGACA+AGG | 0.596291 | 3.3:+65944582 | MS.gene25968:CDS |
| ATAGAGATCAACACATGCAC+GGG | 0.596357 | 3.3:-65947156 | None:intergenic |
| GCTAGGGAGCTCCTCCGTCG+TGG | 0.596909 | 3.3:+65947474 | MS.gene25968:CDS |
| GAAATGAATTGTCTTGTGGC+AGG | 0.597674 | 3.3:+65940947 | MS.gene25968:CDS |
| CGGATGATTTCCTACAACAT+CGG | 0.602117 | 3.3:+65942302 | MS.gene25968:CDS |
| GTTGGTGGTGGTACTTATTG+GGG | 0.602827 | 3.3:+65941806 | MS.gene25968:CDS |
| TCCATGTATAGACTTAAGAC+AGG | 0.602929 | 3.3:+65949246 | MS.gene25968:CDS |
| GCATGGTTTAGATGACCTCA+TGG | 0.603918 | 3.3:+65942905 | MS.gene25968:CDS |
| TGGGGATGGGAAGTTTGAGA+GGG | 0.604314 | 3.3:+65941769 | MS.gene25968:CDS |
| AGTGGGACAAATGTTGGTGG+TGG | 0.605265 | 3.3:+65941794 | MS.gene25968:CDS |
| AGACTTAAGACAGGTCAGCT+CGG | 0.605465 | 3.3:+65949255 | MS.gene25968:CDS |
| TGAAATTCAATCTAATGTTG+AGG | 0.610246 | 3.3:+65939102 | MS.gene25968:CDS |
| ATGGTTGTTGAGAATGGGTG+CGG | 0.610452 | 3.3:+65949220 | MS.gene25968:CDS |
| AGCTATCGACATGCTTGTTG+GGG | 0.611783 | 3.3:+65941985 | MS.gene25968:CDS |
| GTTAGTGGGACAAATGTTGG+TGG | 0.614321 | 3.3:+65941791 | MS.gene25968:CDS |
| CACACTTAAACTGAGCATAG+AGG | 0.616056 | 3.3:-65949443 | None:intergenic |
| CTATGTATTGGATTGCCTGT+AGG | 0.616133 | 3.3:-65939043 | None:intergenic |
| GCTGGAACTACTGGACTTGG+GGG | 0.616473 | 3.3:+65943842 | MS.gene25968:CDS |
| GATAGAGATCAACACATGCA+CGG | 0.617200 | 3.3:-65947157 | None:intergenic |
| CTTGGTATTGACGAATCTGA+GGG | 0.620204 | 3.3:+65938998 | MS.gene25968:CDS |
| TTAGTTGAAAGGTTTCCGAT+GGG | 0.620541 | 3.3:+65942936 | MS.gene25968:CDS |
| TTTGAATGATGTGACTGCAA+TGG | 0.622671 | 3.3:+65948123 | MS.gene25968:CDS |
| GATGCGAGTGCTCTAAATGT+TGG | 0.622823 | 3.3:+65943905 | MS.gene25968:CDS |
| AACTGATGATGCCAAAAGAA+GGG | 0.624687 | 3.3:+65945479 | MS.gene25968:CDS |
| AGAGAGAATGTTAGGGACCG+GGG | 0.628373 | 3.3:+65947368 | MS.gene25968:CDS |
| ATGGGTGCTCCATATACTGG+TGG | 0.639105 | 3.3:+65942954 | MS.gene25968:CDS |
| CCAGTATATGGAGCACCCAT+CGG | 0.641614 | 3.3:-65942951 | None:intergenic |
| TTTATGAGTTACGAAAAGCA+TGG | 0.645096 | 3.3:+65942888 | MS.gene25968:CDS |
| GCTTTGCTAACATCAAGTTG+AGG | 0.645930 | 3.3:-65939079 | None:intergenic |
| TCAAATGAATAGGTTGATGG+AGG | 0.647737 | 3.3:+65946251 | MS.gene25968:intron |
| GAAGCTTTCACACACATGGA+GGG | 0.648007 | 3.3:+65941554 | MS.gene25968:CDS |
| CCTATAGAAGTATAAAGCAG+AGG | 0.649593 | 3.3:+65946358 | MS.gene25968:CDS |
| CAGTAAAGCTCTTCTATGAG+GGG | 0.649740 | 3.3:-65947403 | None:intergenic |
| ATGCAGGCTGTTGCCAGTGA+AGG | 0.652213 | 3.3:+65945453 | MS.gene25968:intron |
| AGATGAAATGAATTGTCTTG+TGG | 0.660802 | 3.3:+65940943 | MS.gene25968:CDS |
| CGGAGGAGCTCCCTAGCCAG+AGG | 0.661956 | 3.3:-65947468 | None:intergenic |
| AGTAAAGCTCTTCTATGAGG+GGG | 0.666765 | 3.3:-65947402 | None:intergenic |
| TGAAGCTTTCACACACATGG+AGG | 0.674064 | 3.3:+65941553 | MS.gene25968:CDS |
| TCACTTCAGTTCCACGACGG+AGG | 0.674137 | 3.3:-65947485 | None:intergenic |
| TTCTCATTCAAATCGCCAAG+TGG | 0.675820 | 3.3:-65942993 | None:intergenic |
| CATTCTCAACAACCATCAAG+GGG | 0.678004 | 3.3:-65949213 | None:intergenic |
| ACTGATGATGCCAAAAGAAG+GGG | 0.688906 | 3.3:+65945480 | MS.gene25968:CDS |
| GTCACGTTACTCAGATACTG+AGG | 0.699781 | 3.3:+65940442 | MS.gene25968:CDS |
| AACTCACTTCAGTTCCACGA+CGG | 0.704303 | 3.3:-65947488 | None:intergenic |
| CAACTAACTGATGCACCATG+AGG | 0.704343 | 3.3:-65942920 | None:intergenic |
| TTTGTGATGAAGGCTACTGG+AGG | 0.733686 | 3.3:+65940860 | MS.gene25968:intron |
| CATTCAAAGCAGCTTCACCG+TGG | 0.733695 | 3.3:+65940260 | MS.gene25968:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 65938956 | 65949476 | 65938956 | ID=MS.gene25968 |
| chr3.3 | mRNA | 65938956 | 65949476 | 65938956 | ID=MS.gene25968.t1;Parent=MS.gene25968 |
| chr3.3 | exon | 65938956 | 65939193 | 65938956 | ID=MS.gene25968.t1.exon1;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65938956 | 65939193 | 65938956 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65940072 | 65940281 | 65940072 | ID=MS.gene25968.t1.exon2;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65940072 | 65940281 | 65940072 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65940435 | 65940487 | 65940435 | ID=MS.gene25968.t1.exon3;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65940435 | 65940487 | 65940435 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65940872 | 65940988 | 65940872 | ID=MS.gene25968.t1.exon4;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65940872 | 65940988 | 65940872 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65941542 | 65941661 | 65941542 | ID=MS.gene25968.t1.exon5;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65941542 | 65941661 | 65941542 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65941764 | 65941858 | 65941764 | ID=MS.gene25968.t1.exon6;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65941764 | 65941858 | 65941764 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65941943 | 65942036 | 65941943 | ID=MS.gene25968.t1.exon7;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65941943 | 65942036 | 65941943 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65942197 | 65942328 | 65942197 | ID=MS.gene25968.t1.exon8;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65942197 | 65942328 | 65942197 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65942611 | 65942723 | 65942611 | ID=MS.gene25968.t1.exon9;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65942611 | 65942723 | 65942611 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65942824 | 65943016 | 65942824 | ID=MS.gene25968.t1.exon10;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65942824 | 65943016 | 65942824 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65943773 | 65943984 | 65943773 | ID=MS.gene25968.t1.exon11;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65943773 | 65943984 | 65943773 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65944420 | 65944464 | 65944420 | ID=MS.gene25968.t1.exon12;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65944420 | 65944464 | 65944420 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65944555 | 65944603 | 65944555 | ID=MS.gene25968.t1.exon13;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65944555 | 65944603 | 65944555 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65945459 | 65945541 | 65945459 | ID=MS.gene25968.t1.exon14;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65945459 | 65945541 | 65945459 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65946263 | 65946379 | 65946263 | ID=MS.gene25968.t1.exon15;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65946263 | 65946379 | 65946263 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65946522 | 65946585 | 65946522 | ID=MS.gene25968.t1.exon16;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65946522 | 65946585 | 65946522 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65947095 | 65947241 | 65947095 | ID=MS.gene25968.t1.exon17;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65947095 | 65947241 | 65947095 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65947345 | 65947503 | 65947345 | ID=MS.gene25968.t1.exon18;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65947345 | 65947503 | 65947345 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65948094 | 65948177 | 65948094 | ID=MS.gene25968.t1.exon19;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65948094 | 65948177 | 65948094 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65949118 | 65949309 | 65949118 | ID=MS.gene25968.t1.exon20;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65949118 | 65949309 | 65949118 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| chr3.3 | exon | 65949405 | 65949476 | 65949405 | ID=MS.gene25968.t1.exon21;Parent=MS.gene25968.t1 |
| chr3.3 | CDS | 65949405 | 65949476 | 65949405 | ID=cds.MS.gene25968.t1;Parent=MS.gene25968.t1 |
| Gene Sequence |
| Protein sequence |
