AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26802


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26802.t1 MTR_4g123700 95.667 300 13 0 1 300 1 300 0.0 558
MS.gene26802.t1 MTR_4g105550 35.034 294 162 10 2 279 10 290 1.02e-49 167
MS.gene26802.t1 MTR_4g132450 32.567 261 149 13 34 276 38 289 4.29e-28 111
MS.gene26802.t1 MTR_5g037310 29.592 294 183 10 13 287 8 296 4.33e-28 110
MS.gene26802.t1 MTR_5g037310 29.592 294 183 10 13 287 8 296 5.44e-28 110
MS.gene26802.t1 MTR_8g090055 29.811 265 160 9 15 264 10 263 6.49e-28 110
MS.gene26802.t1 MTR_2g023970 33.202 253 134 13 34 264 24 263 9.23e-28 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26802.t1 AT3G17350 63.115 244 80 3 32 266 29 271 2.10e-112 327
MS.gene26802.t1 AT3G17350 63.750 240 77 3 34 264 31 269 1.71e-111 324
MS.gene26802.t1 AT5G50290 33.582 268 159 8 13 263 1 266 1.28e-45 156
MS.gene26802.t1 AT1G10380 34.940 249 136 9 34 263 29 270 2.76e-32 121
MS.gene26802.t1 AT1G10380 34.940 249 136 9 34 263 29 270 2.97e-32 121
MS.gene26802.t1 AT1G11915 31.489 235 145 6 34 254 31 263 4.60e-30 116

Find 59 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCCTTTGGATTGGGTTTA+TGG 0.099494 8.2:+10457608 MS.gene26802:CDS
GTTTATGAGTATGAATATTT+TGG 0.176981 8.2:+10457535 MS.gene26802:CDS
GTCCTTTGGATTGGGTTTAT+GGG 0.234143 8.2:+10457609 MS.gene26802:CDS
TTATTCTTTCAAACTCCTTC+TGG 0.239774 8.2:+10457182 MS.gene26802:CDS
AAACCGAATGGGTAGTTTAT+AGG 0.261528 8.2:-10457101 None:intergenic
CACACTTGTGATGAGCTTTA+TGG 0.305918 8.2:+10457431 MS.gene26802:CDS
ATTGAGAGGTGTTGGTCCTT+TGG 0.340253 8.2:+10457595 MS.gene26802:CDS
TCCTTCTGATATCATGCTTC+CGG 0.381010 8.2:-10458246 None:intergenic
CAACTTTCACATCCAGTATC+AGG 0.395675 8.2:-10457653 None:intergenic
AAGGAGTGTACAATGCTCTT+TGG 0.408970 8.2:+10458264 MS.gene26802:CDS
CACAATCTGGTGGGATTTGT+GGG 0.413624 8.2:+10457678 MS.gene26802:CDS
TGGCTTGTAGTAGTTGTAGA+TGG 0.416349 8.2:-10457031 None:intergenic
TAAAACAAAGATATTTGTAA+TGG 0.422370 8.2:-10457397 None:intergenic
AGGTGTTGGTCCTTTGGATT+GGG 0.424565 8.2:+10457601 MS.gene26802:CDS
GAGGTGTTGGTCCTTTGGAT+TGG 0.424668 8.2:+10457600 MS.gene26802:CDS
TGTATCTCAGCTGCTGGAAC+CGG 0.428657 8.2:+10458227 MS.gene26802:intron
ATGGAGCAAGTTGACATTGA+TGG 0.431134 8.2:-10457260 None:intergenic
TATAAACTACCCATTCGGTT+TGG 0.432975 8.2:+10457103 MS.gene26802:CDS
TACCCATTCGGTTTGGAAGA+TGG 0.440399 8.2:+10457110 MS.gene26802:CDS
TATTTGTAATGGTTAAGAAC+AGG 0.444608 8.2:-10457386 None:intergenic
ACACAATCTGGTGGGATTTG+TGG 0.447706 8.2:+10457677 MS.gene26802:CDS
TTCGGTTTGGAAGATGGTTG+TGG 0.449681 8.2:+10457116 MS.gene26802:CDS
GCAGGGTGAGAGTGTGTGAA+TGG 0.463159 8.2:-10457057 None:intergenic
TGCTATTATCCCTCCATCAC+AGG 0.479220 8.2:+10457325 MS.gene26802:CDS
ATTCCTATAAACTACCCATT+CGG 0.492935 8.2:+10457098 MS.gene26802:CDS
ATCATGATGAGGTTGAAGAA+TGG 0.498307 8.2:-10457279 None:intergenic
AACCATCTTCCAAACCGAAT+GGG 0.514516 8.2:-10457112 None:intergenic
TGGAGCAAGTTGACATTGAT+GGG 0.527863 8.2:-10457259 None:intergenic
TGTGAAAGTTGTACACAATC+TGG 0.535884 8.2:+10457665 MS.gene26802:CDS
AGTGATAAATTGAGAGGTGT+TGG 0.537265 8.2:+10457587 MS.gene26802:CDS
ATGAGTTTGAAGATTGTGAA+TGG 0.557897 8.2:-10456996 None:intergenic
CAGGGTGAGAGTGTGTGAAT+GGG 0.561055 8.2:-10457056 None:intergenic
CAAGTTTCACTAATTCAACA+AGG 0.561620 8.2:+10457738 MS.gene26802:CDS
TGAGAGTGTGTGAATGGGAA+TGG 0.572032 8.2:-10457051 None:intergenic
AAAACAGTGTCCTGTGATGG+AGG 0.573412 8.2:-10457335 None:intergenic
ACTAATTCAACAAGGGAATG+TGG 0.573682 8.2:+10457746 MS.gene26802:CDS
TATAGGAATGGTACCACATG+AGG 0.575471 8.2:-10457084 None:intergenic
CAACCATCTTCCAAACCGAA+TGG 0.581667 8.2:-10457113 None:intergenic
CTACAATAAGCAAAACCACT+TGG 0.586476 8.2:-10458295 None:intergenic
ATCCCATAAACCCAATCCAA+AGG 0.604071 8.2:-10457611 None:intergenic
ACCGGAAGCATGATATCAGA+AGG 0.604148 8.2:+10458245 MS.gene26802:CDS
TGTACAATGCTCTTTGGACA+CGG 0.610218 8.2:+10458270 MS.gene26802:CDS
GAATGGGTAGTTTATAGGAA+TGG 0.619390 8.2:-10457096 None:intergenic
TGAACTTTGTAAGAACCAGA+AGG 0.628532 8.2:-10457197 None:intergenic
TCACCCTGCAAAACCTCATG+TGG 0.633355 8.2:+10457071 MS.gene26802:CDS
AAACAGTGTCCTGTGATGGA+GGG 0.633615 8.2:-10457334 None:intergenic
TTTAATAGTGATAAATTGAG+AGG 0.647028 8.2:+10457581 MS.gene26802:CDS
TTGAACATGTTTCTGAACTG+TGG 0.654643 8.2:-10457143 None:intergenic
TTATAGCTCGTGAAACAACA+AGG 0.671730 8.2:-10457503 None:intergenic
TCTTTGGACACGGCACCAAG+TGG 0.673512 8.2:+10458280 MS.gene26802:CDS
TGATGGGTCGTAGAGTACCA+TGG 0.674328 8.2:-10457243 None:intergenic
ACTTGTGATGAGCTTTATGG+TGG 0.679797 8.2:+10457434 MS.gene26802:CDS
GGGTTTGATACTGAAACTGA+AGG 0.680082 8.2:+10457698 MS.gene26802:CDS
AAGTTTCACTAATTCAACAA+GGG 0.686221 8.2:+10457739 MS.gene26802:CDS
GAAAGTTGTACACAATCTGG+TGG 0.691833 8.2:+10457668 MS.gene26802:CDS
GTCATGAGAAAATCATGATG+AGG 0.693658 8.2:-10457290 None:intergenic
AAAGTTGTACACAATCTGGT+GGG 0.724462 8.2:+10457669 MS.gene26802:CDS
AGTTTGAAAGAATAACTCAG+TGG 0.732725 8.2:-10457174 None:intergenic
TAGCTCGTGAAACAACAAGG+TGG 0.804087 8.2:-10457500 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTTTAGAAATTAAAA+AGG + chr8.2:10457904-10457923 MS.gene26802:intron 10.0%
!! TAAAACAAAGATATTTGTAA+TGG - chr8.2:10457400-10457419 None:intergenic 15.0%
!! TTTAATAGTGATAAATTGAG+AGG + chr8.2:10457581-10457600 MS.gene26802:CDS 20.0%
!!! GTTTATGAGTATGAATATTT+TGG + chr8.2:10457535-10457554 MS.gene26802:CDS 20.0%
!!! TATCTTTGTTTTAACTTTGA+AGG + chr8.2:10457407-10457426 MS.gene26802:CDS 20.0%
!!! TATTTGGATTTTGAATCATT+TGG + chr8.2:10457789-10457808 MS.gene26802:intron 20.0%
! AAATAGAATACGGAAACTTA+CGG - chr8.2:10458207-10458226 None:intergenic 25.0%
! AAGTTTCACTAATTCAACAA+GGG + chr8.2:10457739-10457758 MS.gene26802:CDS 25.0%
! CTGAGATACAAAATAGAATA+CGG - chr8.2:10458217-10458236 None:intergenic 25.0%
! TATTTGTAATGGTTAAGAAC+AGG - chr8.2:10457389-10457408 None:intergenic 25.0%
!!! CTTTTTTAGTTGATAACAGT+AGG + chr8.2:10458071-10458090 MS.gene26802:intron 25.0%
!!! TAAGTTTTATCTGAGTTGTT+AGG + chr8.2:10458099-10458118 MS.gene26802:intron 25.0%
AGTTTGAAAGAATAACTCAG+TGG - chr8.2:10457177-10457196 None:intergenic 30.0%
ATGAGTTTGAAGATTGTGAA+TGG - chr8.2:10456999-10457018 None:intergenic 30.0%
ATTCCTATAAACTACCCATT+CGG + chr8.2:10457098-10457117 MS.gene26802:CDS 30.0%
CAAGTTTCACTAATTCAACA+AGG + chr8.2:10457738-10457757 MS.gene26802:CDS 30.0%
CTACTACTTGGTTTCTTATT+TGG + chr8.2:10457773-10457792 MS.gene26802:intron 30.0%
GGTGCTAGTTAATATGAATA+GGG + chr8.2:10457810-10457829 MS.gene26802:intron 30.0%
TGACTACAACAAAAAAACCA+TGG + chr8.2:10457226-10457245 MS.gene26802:CDS 30.0%
TTATTCTTTCAAACTCCTTC+TGG + chr8.2:10457182-10457201 MS.gene26802:CDS 30.0%
! AGTTTTTCTTCACAAGATGA+GGG + chr8.2:10458316-10458335 MS.gene26802:CDS 30.0%
! TAGTTTTTCTTCACAAGATG+AGG + chr8.2:10458315-10458334 MS.gene26802:CDS 30.0%
! TCACCAACAACAATTTTGAA+AGG - chr8.2:10458039-10458058 None:intergenic 30.0%
! TGGTGCTAGTTAATATGAAT+AGG + chr8.2:10457809-10457828 MS.gene26802:intron 30.0%
!! CACCAACAACAATTTTGAAA+GGG - chr8.2:10458038-10458057 None:intergenic 30.0%
!!! GGTTTTTTTGTTGTAGTCAA+TGG - chr8.2:10457225-10457244 None:intergenic 30.0%
AAACCGAATGGGTAGTTTAT+AGG - chr8.2:10457104-10457123 None:intergenic 35.0%
AAAGTTGTACACAATCTGGT+GGG + chr8.2:10457669-10457688 MS.gene26802:CDS 35.0%
ACTAATTCAACAAGGGAATG+TGG + chr8.2:10457746-10457765 MS.gene26802:CDS 35.0%
ATCATGATGAGGTTGAAGAA+TGG - chr8.2:10457282-10457301 None:intergenic 35.0%
CACCCTTTCAAAATTGTTGT+TGG + chr8.2:10458033-10458052 MS.gene26802:intron 35.0%
CTACAATAAGCAAAACCACT+TGG - chr8.2:10458298-10458317 None:intergenic 35.0%
GAAAATCATACAGCACTAAC+TGG + chr8.2:10457926-10457945 MS.gene26802:intron 35.0%
GAATGGGTAGTTTATAGGAA+TGG - chr8.2:10457099-10457118 None:intergenic 35.0%
GTCATGAGAAAATCATGATG+AGG - chr8.2:10457293-10457312 None:intergenic 35.0%
TATAAACTACCCATTCGGTT+TGG + chr8.2:10457103-10457122 MS.gene26802:CDS 35.0%
TGAACTTTGTAAGAACCAGA+AGG - chr8.2:10457200-10457219 None:intergenic 35.0%
TGTGAAAGTTGTACACAATC+TGG + chr8.2:10457665-10457684 MS.gene26802:CDS 35.0%
TTATAGCTCGTGAAACAACA+AGG - chr8.2:10457506-10457525 None:intergenic 35.0%
! AGTGATAAATTGAGAGGTGT+TGG + chr8.2:10457587-10457606 MS.gene26802:CDS 35.0%
! GTTTTTCTTCACAAGATGAG+GGG + chr8.2:10458317-10458336 MS.gene26802:CDS 35.0%
! TTGAACATGTTTCTGAACTG+TGG - chr8.2:10457146-10457165 None:intergenic 35.0%
! TTTTTCTTCACAAGATGAGG+GGG + chr8.2:10458318-10458337 MS.gene26802:CDS 35.0%
AACCATCTTCCAAACCGAAT+GGG - chr8.2:10457115-10457134 None:intergenic 40.0%
ACAAAAACAGTGTCCTGTGA+TGG - chr8.2:10457341-10457360 None:intergenic 40.0%
ATCCCATAAACCCAATCCAA+AGG - chr8.2:10457614-10457633 None:intergenic 40.0%
CAACTTTCACATCCAGTATC+AGG - chr8.2:10457656-10457675 None:intergenic 40.0%
CACACTTGTGATGAGCTTTA+TGG + chr8.2:10457431-10457450 MS.gene26802:CDS 40.0%
GAAAGTTGTACACAATCTGG+TGG + chr8.2:10457668-10457687 MS.gene26802:CDS 40.0%
GAATACGGAAACTTACGGTT+AGG - chr8.2:10458202-10458221 None:intergenic 40.0%
GAATGTGGTAAGCTACTACT+TGG + chr8.2:10457761-10457780 MS.gene26802:intron 40.0%
GGGTTTGATACTGAAACTGA+AGG + chr8.2:10457698-10457717 MS.gene26802:CDS 40.0%
TATAGGAATGGTACCACATG+AGG - chr8.2:10457087-10457106 None:intergenic 40.0%
TCCTTCTGATATCATGCTTC+CGG - chr8.2:10458249-10458268 None:intergenic 40.0%
TGTACAATGCTCTTTGGACA+CGG + chr8.2:10458270-10458289 MS.gene26802:CDS 40.0%
TTGCTTCGAGCTTAGAGTTA+AGG - chr8.2:10458159-10458178 None:intergenic 40.0%
! AAGGAGTGTACAATGCTCTT+TGG + chr8.2:10458264-10458283 MS.gene26802:CDS 40.0%
! ACTTGTGATGAGCTTTATGG+TGG + chr8.2:10457434-10457453 MS.gene26802:CDS 40.0%
! ATGGAGCAAGTTGACATTGA+TGG - chr8.2:10457263-10457282 None:intergenic 40.0%
! ATGGTGGTTGCAATGCTTTT+AGG + chr8.2:10457450-10457469 MS.gene26802:CDS 40.0%
! CTATTTTGTATCTCAGCTGC+TGG + chr8.2:10458221-10458240 MS.gene26802:intron 40.0%
! GTCCTTTGGATTGGGTTTAT+GGG + chr8.2:10457609-10457628 MS.gene26802:CDS 40.0%
! TCTTTTAGTGTGCCTGATAC+TGG + chr8.2:10457641-10457660 MS.gene26802:CDS 40.0%
! TGGAGCAAGTTGACATTGAT+GGG - chr8.2:10457262-10457281 None:intergenic 40.0%
! TGGCTTGTAGTAGTTGTAGA+TGG - chr8.2:10457034-10457053 None:intergenic 40.0%
! TGGTGGTTGCAATGCTTTTA+GGG + chr8.2:10457451-10457470 MS.gene26802:CDS 40.0%
AAAACAGTGTCCTGTGATGG+AGG - chr8.2:10457338-10457357 None:intergenic 45.0%
ACACAATCTGGTGGGATTTG+TGG + chr8.2:10457677-10457696 MS.gene26802:CDS 45.0%
CAACCATCTTCCAAACCGAA+TGG - chr8.2:10457116-10457135 None:intergenic 45.0%
CACAATCTGGTGGGATTTGT+GGG + chr8.2:10457678-10457697 MS.gene26802:CDS 45.0%
GCAATTGAGCTACCAAAACC+TGG + chr8.2:10458175-10458194 MS.gene26802:intron 45.0%
TAGCTCGTGAAACAACAAGG+TGG - chr8.2:10457503-10457522 None:intergenic 45.0%
TGCTATTATCCCTCCATCAC+AGG + chr8.2:10457325-10457344 MS.gene26802:CDS 45.0%
! AAACAGTGTCCTGTGATGGA+GGG - chr8.2:10457337-10457356 None:intergenic 45.0%
! ACCGGAAGCATGATATCAGA+AGG + chr8.2:10458245-10458264 MS.gene26802:CDS 45.0%
! GGTCCTTTGGATTGGGTTTA+TGG + chr8.2:10457608-10457627 MS.gene26802:CDS 45.0%
! GTACCACATGAGGTTTTGCA+GGG - chr8.2:10457077-10457096 None:intergenic 45.0%
! TGAGAGTGTGTGAATGGGAA+TGG - chr8.2:10457054-10457073 None:intergenic 45.0%
! TTACGGTTAGGACCAGGTTT+TGG - chr8.2:10458190-10458209 None:intergenic 45.0%
!! AGGTGTTGGTCCTTTGGATT+GGG + chr8.2:10457601-10457620 MS.gene26802:CDS 45.0%
!! ATTGAGAGGTGTTGGTCCTT+TGG + chr8.2:10457595-10457614 MS.gene26802:CDS 45.0%
!! TACCCATTCGGTTTGGAAGA+TGG + chr8.2:10457110-10457129 MS.gene26802:CDS 45.0%
!! TTCGGTTTGGAAGATGGTTG+TGG + chr8.2:10457116-10457135 MS.gene26802:CDS 45.0%
GGAAACTTACGGTTAGGACC+AGG - chr8.2:10458196-10458215 None:intergenic 50.0%
TCACCCTGCAAAACCTCATG+TGG + chr8.2:10457071-10457090 MS.gene26802:CDS 50.0%
TGTATCTCAGCTGCTGGAAC+CGG + chr8.2:10458227-10458246 MS.gene26802:intron 50.0%
! CAGGGTGAGAGTGTGTGAAT+GGG - chr8.2:10457059-10457078 None:intergenic 50.0%
! GGTACCACATGAGGTTTTGC+AGG - chr8.2:10457078-10457097 None:intergenic 50.0%
! TGATGGGTCGTAGAGTACCA+TGG - chr8.2:10457246-10457265 None:intergenic 50.0%
!! GAGGTGTTGGTCCTTTGGAT+TGG + chr8.2:10457600-10457619 MS.gene26802:CDS 50.0%
TCTTTGGACACGGCACCAAG+TGG + chr8.2:10458280-10458299 MS.gene26802:CDS 55.0%
! GCAGGGTGAGAGTGTGTGAA+TGG - chr8.2:10457060-10457079 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 10456978 10458349 10456978 ID=MS.gene26802
chr8.2 mRNA 10456978 10458349 10456978 ID=MS.gene26802.t1;Parent=MS.gene26802
chr8.2 exon 10456978 10457767 10456978 ID=MS.gene26802.t1.exon1;Parent=MS.gene26802.t1
chr8.2 CDS 10456978 10457767 10456978 ID=cds.MS.gene26802.t1;Parent=MS.gene26802.t1
chr8.2 exon 10458237 10458349 10458237 ID=MS.gene26802.t1.exon2;Parent=MS.gene26802.t1
chr8.2 CDS 10458237 10458349 10458237 ID=cds.MS.gene26802.t1;Parent=MS.gene26802.t1
Gene Sequence

>MS.gene26802

ATGCATCATCTTCTAACACCATTCACAATCTTCAAACTCATTATACTAACACTCCATCTACAACTACTACAAGCCATTCCCATTCACACACTCTCACCCTGCAAAACCTCATGTGGTACCATTCCTATAAACTACCCATTCGGTTTGGAAGATGGTTGTGGAGCACCACAGTTCAGAAACATGTTCAACTGCACAACCACTGAGTTATTCTTTCAAACTCCTTCTGGTTCTTACAAAGTTCAATCCATTGACTACAACAAAAAAACCATGGTACTCTACGACCCATCAATGTCAACTTGCTCCATTCTTCAACCTCATCATGATTTTCTCATGACAGATATTCAATCTGCTATTATCCCTCCATCACAGGACACTGTTTTTGTTCTGTTAAATTGCTCTATAGACTCTCCTGTTCTTAACCATTACAAATATCTTTGTTTTAACTTTGAAGGACACACTTGTGATGAGCTTTATGGTGGTTGCAATGCTTTTAGGGTGTTTCATTTGATGACAAATAGCTCACCACCTTGTTGTTTCACGAGCTATAATACTGTGAAGTTTATGAGTATGAATATTTTGGATTGTACACATTATACTAGTGTGTTTAATAGTGATAAATTGAGAGGTGTTGGTCCTTTGGATTGGGTTTATGGGATTAAGCTTTCTTTTAGTGTGCCTGATACTGGATGTGAAAGTTGTACACAATCTGGTGGGATTTGTGGGTTTGATACTGAAACTGAAGGTCTTCTTTGTCTCTGCTCAAGTTTCACTAATTCAACAAGGGAATGTGGTAAGCTACTACTTGGTTTCTTATTTGGATTTTGAATCATTTGGTGCTAGTTAATATGAATAGGGTTGTATAGCACACTAATTTGAAAGAAAAAAATAAGAAATTGTTTTTCAAAACTAGTTAATCAATTTTTCTTTTCTTTTTTAGAAATTAAAAAGGAAAATCATACAGCACTAACTGGTTTTAGAGTGAAATGTTAGTATATTATAGATGCAAGAACTAAACTTAGACGCATTTTGTTTTCTTTCAAAAGACAACTTTTTTACACCCTTTCAAAATTGTTGTTGGTGACAATGATACTATCTTTTTTAGTTGATAACAGTAGGAATACTAAGTTTTATCTGAGTTGTTAGGTTAATATTTTCTTCTTTATCAACTCTTTCAACATCCTTAACTCTAAGCTCGAAGCAATTGAGCTACCAAAACCTGGTCCTAACCGTAAGTTTCCGTATTCTATTTTGTATCTCAGCTGCTGGAACCGGAAGCATGATATCAGAAGGAGTGTACAATGCTCTTTGGACACGGCACCAAGTGGTTTTGCTTATTGTAGTTTTTCTTCACAAGATGAGGGGGCTAATTTGA

Protein sequence

>MS.gene26802.t1

MHHLLTPFTIFKLIILTLHLQLLQAIPIHTLSPCKTSCGTIPINYPFGLEDGCGAPQFRNMFNCTTTELFFQTPSGSYKVQSIDYNKKTMVLYDPSMSTCSILQPHHDFLMTDIQSAIIPPSQDTVFVLLNCSIDSPVLNHYKYLCFNFEGHTCDELYGGCNAFRVFHLMTNSSPPCCFTSYNTVKFMSMNILDCTHYTSVFNSDKLRGVGPLDWVYGIKLSFSVPDTGCESCTQSGGICGFDTETEGLLCLCSSFTNSTRECAAGTGSMISEGVYNALWTRHQVVLLIVVFLHKMRGLI